Biosensor detection systems, methods and related products

Abstract

The present application provides a biosensor detection system, method and related products. The biosensor detection system includes: a light emitter, a first light transmission section, an optical fiber connector, a detection sensing section, a second light transmission section and a signal processing device. The detection sensing section includes a biodetection sensor. The biodetection sensor includes a light incident part, a first sensing part, a transition transmission part, a second sensing part and a light emitting part. By setting the shape of the biodetection sensor to a "U" shape, the incident light of the light emitter can pass through the first sensing part and the second sensing part in sequence, and can detect the temperature of the target detection solution while also detecting the concentration of the target molecule in the target detection solution, which is beneficial to improving the detection efficiency. At the same time, it can also eliminate the interference of the background liquid concentration, which is beneficial to improving the accuracy of the detection.

Description

Technical Field

[0001] The present application relates to the field of biological detection technology, and in particular to a biological sensing detection system, method and related products. Background Art

[0002] SPR (Surface plasmon resonance) detection technology is a new biochemical analysis method that is extremely sensitive to tiny changes in refractive index. Any biochemical reaction will inevitably cause tiny changes in the refractive index of the detection environment. Therefore, this technology is used in the fields of biochemistry, biophysics and biomedicine.

[0003] However, some biochemical reactions occur at specific temperatures. If the reaction status of the biochemical reaction needs to be detected, the temperature of the detection solution needs to be known. However, currently, a single SPR sensor cannot simultaneously detect the temperature and the concentration of the molecule to be detected. Summary of the invention

[0004] In view of this, the present application provides a biosensor detection system, method and related products, which are realized by setting the shape of the biosensor to a "U" shape so that the incident light of the light emitter can pass through the first sensing part and the second sensing part in sequence, and can detect the temperature of the target detection solution while also detecting the concentration of the target molecules in the target detection solution, which is beneficial to improving the detection efficiency. At the same time, it can also eliminate the interference of the background liquid concentration, which is beneficial to improving the accuracy of the detection.

[0005] In a first aspect, the present application provides a biosensor detection system, the biosensor detection system comprising:

[0006] A light transmitter; a first light transmission segment; a fiber optic connector, wherein the first light transmission segment is connected to a first port of the fiber optic connector; a second light transmission segment, wherein one end of the second light transmission segment is connected to a second port of the fiber optic connector;

[0007] A detection sensing section, wherein the detection sensing section comprises a biological detection sensor, and the biological detection sensor comprises a light incident part, a first sensing part, a transition transmission part, a second sensing part and a light emitting part; two ends of the light incident part are respectively connected to the third port of the optical fiber connector and one end of the first sensing part, two ends of the light emitting part are respectively connected to the fourth port of the optical fiber connector and one end of the second sensing part, and two ends of the transition transmission part are respectively connected to the other end of the first sensing part and the other end of the second sensing part; the first light transmission section is connected to the light incident part through the first port and the third port, and the second light transmission section is connected to the light emitting part through the second port and the fourth port; the first metal film of the first sensing part is modified with an adsorption functional layer, and the second metal film of the second sensing part is modified with a temperature sensitive layer;

[0008] A signal processing device, the signal processing device is connected to the other end of the second light transmission segment; the signal processing device is used to collect a reference spectrum signal of the first output light from the biological detection sensor, collect a standard spectrum signal of the second output light from the biological detection sensor, determine a first resonance peak wavelength of the reference spectrum signal and a second resonance peak wavelength of the standard spectrum signal, determine a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine the temperature of a target detection solution according to a set of mapping relationships between the first target wavelength value and the first wavelength value, the reference spectrum signal is a detection signal of the biological detection sensor in the reference detection solution, the standard spectrum signal is a detection signal of the biological detection sensor in the target detection solution and under the steady state and other conditions when the adsorption functional layer adsorbs the target molecules in the target detection solution, the first target wavelength value is used to characterize the change in the absorption capacity of the biological detection sensor for the incident light of the light emitter under the temperature constraint of the target detection solution.

[0009] In one possible example, the signal processing device is used to determine the third resonance peak wavelength of the reference spectral signal, determine the fourth resonance peak wavelength of the standard spectral signal, determine a second target wavelength value based on the third resonance peak wavelength and the fourth resonance peak wavelength, and determine the concentration of the target molecule in the target detection solution based on a set of mapping relationships between the second target wavelength value and the second wavelength value, wherein the second target wavelength value is used to characterize the change in the absorption capacity of the biological detection sensor to the incident light under steady-state constraints when the adsorption functional layer adsorbs the target molecule.

[0010] In a possible example, the first resonance peak wavelength is greater than the third resonance peak wavelength, and the second resonance peak wavelength is greater than the fourth resonance peak wavelength; or,

[0011] The first resonance peak wavelength is shorter than the third resonance peak wavelength, and the second resonance peak wavelength is shorter than the fourth resonance peak wavelength.

[0012] In a possible example, the temperature sensitive layer includes a temperature sensitive material, and the temperature sensitive material includes at least one of the following: polydimethylsiloxane, indium tin oxide, silver, and liquid crystal; the temperature sensitive layer is used for isolating the detection solution of the second sensing part;

[0013] The adsorption functional layer includes a sealing agent and a target targeting object of the target molecule, and the sealing agent is used to seal the area on the first metal film where the target targeting object is not modified.

[0014] In a possible example, the first target wavelength value is a relative difference value or a division result value between the first resonance peak wavelength and the second resonance peak wavelength;

[0015] The second target wavelength value is a relative difference between the third resonance peak wavelength and the fourth resonance peak wavelength or a division result value;

[0016] The first wavelength value mapping relationship set includes a corresponding relationship between the first wavelength value and the temperature of the target detection solution, and the absolute value of the first wavelength value is positively correlated with the temperature of the target detection solution;

[0017] The second wavelength value mapping relationship set includes a corresponding relationship between the second wavelength value and the concentration of the target molecule in the target detection solution, and the second wavelength value is positively correlated with the concentration of the target molecule.

[0018] In a possible example, the transition transmission portion is bent, the light incident portion and the light emitting portion are symmetrically arranged, and the first sensing portion and the second sensing portion are symmetrically arranged.

[0019] In a possible example, the incident light has the same first transmission direction in the light incident portion and the transition transmission portion, and the incident light has the same second transmission direction in the light exit portion and the transition transmission portion, and the first transmission direction and the second transmission direction are opposite.

[0020] In a possible example, the light incident portion includes a first optical fiber, the first optical fiber includes a first cladding and a first fiber core, and one end of the first fiber core is connected to the third port of the optical fiber connector;

[0021] The first sensing part includes a second optical fiber and the first metal film wrapped on the surface of the second optical fiber, and one end of the second optical fiber is connected to the other end of the first fiber core;

[0022] The transition transmission part includes a third optical fiber, the third optical fiber includes a second cladding and a second core, and one end of the second core is connected to the other end of the second optical fiber;

[0023] The second sensing part includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, and one end of the fourth optical fiber is connected to the other end of the second fiber core;

[0024] The light emitting portion includes a fifth optical fiber, the fifth optical fiber includes a third cladding and a third core, one end of the third core is connected to the other end of the fourth optical fiber, and the other end of the third core is connected to the fourth port of the optical fiber connector.

[0025] In a second aspect, the present application further provides a biosensor detection method, which is applied to a signal processing device in a biosensor detection system, wherein the biosensor detection system includes a light emitter, a first light transmission segment, an optical fiber connector, a second light transmission segment, a detection sensing segment and the signal processing device, wherein the detection sensing segment includes a biodetection sensor, and the biodetection sensor includes a first sensing part and a second sensing part, wherein the first light transmission segment is connected to a first port of the optical fiber connector, one end of the second light transmission segment is connected to a second port of the optical fiber connector, and the two ends of the biodetection sensor are respectively connected to a third port and a fourth port of the optical fiber connector, the first metal film of the first sensing part is modified with an adsorption functional layer, the second metal film of the second sensing part is modified with a temperature sensitive layer, and the signal processing device is connected to the second light transmission segment; the method includes:

[0026] Acquire a reference spectral signal of the first outgoing light from the biological detection sensor, and acquire a standard spectral signal of the second outgoing light from the biological detection sensor, wherein the reference spectral signal is a detection signal of the biological detection sensor in a reference detection solution, and the standard spectral signal is a detection signal of the biological detection sensor in the target detection solution and when the adsorption functional layer adsorbs target molecules in the target detection solution in a steady state and under other conditions;

[0027] Determine a first resonance peak wavelength and a third resonance peak wavelength according to the reference spectrum signal;

[0028] Determine the second resonance peak wavelength and the fourth resonance peak wavelength according to the standard spectrum signal;

[0029] Determine a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine a second target wavelength value according to the third resonance peak wavelength and the fourth resonance peak wavelength, wherein the first target wavelength value is used to characterize the change in the absorption capacity of the biosensor to the incident light of the light emitter under the temperature constraint of the target detection solution, and the second target wavelength value is used to characterize the change in the absorption capacity of the biosensor to the incident light under the condition of steady-state constraint when the adsorption functional layer adsorbs the target molecule;

[0030] The temperature of the target detection solution is determined according to the first target wavelength value and the first wavelength value mapping relationship set, and the concentration of the target molecule in the target detection solution is determined according to the second target wavelength value and the second wavelength value mapping relationship set.

[0031] In one possible example, the interaction instrument is connected to the signal processing device, and the interaction instrument is used to obtain the first target characterization value and the second target characterization value from the signal processing device, and analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule based on the first target characterization value and the second target characterization value.

[0032] In a third aspect, the present application also provides a portable fiber optic biological detection device, which includes a shell, a display screen and the biological sensor detection system. The biological sensor detection system is accommodated in the shell, and the display screen is arranged outside the shell and is used to display detection information.

[0033] The biosensor detection system provided in this embodiment is achieved by modifying the first metal film of the first sensing part with an adsorption functional layer for adsorbing target molecules, and modifying the second metal film of the second sensing part with a temperature sensitive layer, and setting the shape of the biodetection sensor to a "U" shape, so that the incident light of the light emitter can pass through the first sensing part and the second sensing part in sequence, and can detect the temperature of the target detection solution while also detecting the concentration of the target molecules in the target detection solution, which is beneficial to improving the detection efficiency and accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1is a structural schematic diagram of a biosensor detection system provided in an embodiment of the present application;

[0036] Figure 2 is a schematic structural diagram of a biological detection sensor provided in an embodiment of the present application;

[0037] Figure 3 is a schematic diagram of a light transmission path provided in an embodiment of the present application;

[0038] Figure 4 is a schematic diagram of another biological detection sensor provided in an embodiment of the present application;

[0039] Figure 5A is a schematic diagram of a reference spectrum provided in an embodiment of the present application;

[0040] Figure 5B is a schematic diagram of another reference spectrum provided in an embodiment of the present application;

[0041] Fig. 6A is a schematic diagram of a standard spectrum provided in an embodiment of the present application;

[0042] Figure 6B is a schematic diagram of another standard spectrum diagram provided in an embodiment of the present application;

[0043] Figure 7 is a schematic structural diagram of another biosensor detection system provided in an embodiment of the present application;

[0044] Figure 8 is a structural schematic diagram of another biosensor detection system provided in an embodiment of the present application;

[0045] Fig. 9 It is a flow chart of a biosensor detection method provided in an embodiment of the present application.

[0046] Description of reference numerals:

[0047] Biosensing detection system 1, light emitter 10, first light transmission section 20, optical fiber adapter 30, detection sensing section 40, second light transmission section 50, signal processing device 60, biosensing sensor 41, light incident part 411, first sensing part 412, transition transmission part 413, second sensing part 414, light emitting part 415, first port 31, second port 32, third port 33, fourth port 34, first optical fiber 4110, first cladding 4111, first core 4112, second optical fiber 4113, Fiber 4120, first metal film 4121, third optical fiber 4130, second cladding 4131, second core 4132, fourth optical fiber 4140, second metal film 4141, fifth optical fiber 4150, third cladding 4151, third core 4152, adsorption functional layer 4213, temperature sensitive layer 4142, biological detection channel 70, transmission switch 80, interaction instrument 90, first connection area 4113, second connection area 4133, third connection area 4134, fourth connection area 4153. DETAILED DESCRIPTION

[0048] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0049] The terms "first", "second", etc. in the specification and claims of this application and the above-mentioned drawings are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0050] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0051] In the embodiments of the present application, "and / or" describes the association relationship of the associated objects, indicating that three relationships may exist. For example, A and / or B can represent the following three situations: A exists alone; A and B exist at the same time; B exists alone. Among them, A and B can be singular or plural.

[0052] In the embodiment of the present application, the symbol " / " can indicate that the objects associated with each other are in an "or" relationship. In addition, the symbol " / " can also indicate a division sign, that is, performing a division operation. For example, A / B can indicate A divided by B.

[0053] In the embodiments of the present application, "at least one item" or similar expressions refer to any combination of these items, including any combination of single items or plural items, and refer to one or more, and multiple refers to two or more. For example, at least one item of a, b, or c can represent the following seven situations: a, b, c, a and b, a and c, b and c, a, b, and c. Among them, each of a, b, and c can be an element or a set containing one or more elements.

[0054] In the embodiments of the present application, "equal to" can be used in conjunction with greater than, and is applicable to the technical solution adopted when greater than, and can also be used in conjunction with less than, and is applicable to the technical solution adopted when less than. When equal to is used in conjunction with greater than, it is not used in conjunction with less than; when equal to is used in conjunction with less than, it is not used in conjunction with greater than.

[0055] In order to better understand the solutions of the embodiments of the present application, the electronic devices, related concepts and backgrounds that may be involved in the embodiments of the present application are first introduced below.

[0056] The electronic devices involved in the embodiments of the present application may include various handheld devices with wireless communication functions, vehicle-mounted devices, wearable devices, computing devices or other processing devices connected to a wireless modem, as well as various forms of user equipment (UE), mobile stations (MS), electronic devices (terminal devices), etc. For the convenience of description, the above-mentioned devices are collectively referred to as electronic devices. The electronic device can also be a spectral detector in an optical fiber biological detection system.

[0057] See also Figure 1 and Figure 2 , Figure 1 is a schematic diagram of the structure of a biosensor detection system provided in an embodiment of the present application, Figure 21 is a schematic diagram of the structure of a biological detection sensor provided in an embodiment of the present application. The biological sensing detection system 1 of this embodiment includes a light emitter 10, a first light transmission section 20, an optical fiber adapter 30, a detection sensing section 40, a second light transmission section 50 and a signal processing device 60, the detection sensing section 40 includes a biological detection sensor 41, and the biological detection sensor 41 includes a light incident part 411, a first sensing part 412, a transition transmission part 413, a second sensing part 414 and a light emitting part 415.

[0058] The optical fiber adapter 30 includes a first port 31, a second port 32, a third port 33 and a fourth port 34. The first port 31 is connected to the third port 33, and the second port 32 is connected to the fourth port 34. The first port 31 and the second port 32 are spaced apart.

[0059] Among them, the light emitted by the light emitter 10 is broadband white light, one end of the first light transmission segment 20 is connected to the first port 31, and the light emitter 10 is located at the other end of the first light transmission segment 20; one end of the second light transmission segment 50 is connected to the second port 32 of the optical fiber connector 30, the first light transmission segment 20 is connected to the first port 31 of the optical fiber connector 30, and the signal processing device 60 is connected to the other end of the second light transmission segment 50.

[0060] Among them, broadband white light can be understood as a composite light mixed from multiple monochromatic lights, and its spectral range covers most or all of the visible light bands.

[0061] Among them, the two ends of the light incident part 411 are respectively connected to the third port 33 of the optical fiber connector 30 and one end of the first sensing part 412, the two ends of the light emitting part 415 are respectively connected to the fourth port 34 of the optical fiber connector 30 and one end of the second sensing part 414, and the two ends of the transition transmission part 413 are respectively connected to the other end of the first sensing part 412 and the other end of the second sensing part 414; the first light transmission segment 20 is connected to the light incident part 411 through the first port 31 and the third port 33, and the second light transmission segment 50 is connected to the light emitting part 415 through the second port 32 and the fourth port 34; the first metal film 4121 of the first sensing part 412 is modified with an adsorption functional layer 4213, and the second metal film 4141 of the second sensing part 414 is modified with a temperature sensitive layer 4142.

[0062] The target molecule may be an antigen, and the target targeting substance may be an antibody, which is not limited here. The target targeting substance may react with the target molecule to achieve stable adsorption of the target molecule by the first metal film 4121 of the first sensing part 412 .

[0063] The temperature sensitive layer 4142 includes a temperature sensitive material, and the temperature sensitive material includes at least one of the following: polydimethylsiloxane, indium tin oxide, silver, and liquid crystal; the temperature sensitive layer 4142 is used to isolate the detection solution of the second sensing part 414, so that the refractive index of the surface of the second metal film 4141 is only affected by temperature. The temperature sensitive layer 4142 will expand and contract with heat, resulting in a change in the refractive index of the surface of the second metal film 4141 of the second sensing part 414. The adsorption functional layer 4213 includes a sealing agent and a target target of a target molecule, and the sealing agent is used to seal the area of ​​the unmodified target target on the first metal film 4121. The adsorption of target molecules by the adsorption functional layer 4213 will cause the refractive index of the surface of the first metal film 4121 of the first sensing part 412 to change.

[0064] The components of the blocking agent include but are not limited to bovine serum albumin.

[0065] Among them, the signal processing device 60 is used to collect a reference spectral signal of the first outgoing light from the biological detection sensor 41, collect a standard spectral signal of the second outgoing light from the biological detection sensor 41, determine the first resonance peak wavelength of the reference spectral signal and the second resonance peak wavelength of the standard spectral signal, determine a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine the temperature of the target detection solution according to a set of mapping relationships between the first target wavelength value and the first wavelength value. The reference spectral signal is a detection signal of the biological detection sensor 41 in the reference detection solution, and the standard spectral signal is a detection signal of the biological detection sensor 41 in the target detection solution and when the adsorption functional layer 4213 adsorbs the target molecules in the target detection solution in a steady state and under other conditions. The first target wavelength value is used to characterize the change in the absorption capacity of the biological detection sensor 41 for the incident light of the light emitter 10 under the temperature constraint of the target detection solution.

[0066] Among them, other conditions include temperature conditions and background solution concentration conditions. The reference detection solution is a blank solution, such as a buffer solution, and the concentration of the blank solution is the same as that of the target detection solution, and the temperature is the same or different.

[0067] The signal processing device 60 may be a spectral detector or other spectral signal processing device, which is not limited here.

[0068] Among them, the signal processing device 60 is also used to determine the third resonance peak wavelength of the reference spectrum signal, determine the fourth resonance peak wavelength of the standard spectrum signal, determine the second target wavelength value according to the third resonance peak wavelength and the fourth resonance peak wavelength, and determine the concentration of the target molecules in the target detection solution according to the second target wavelength value and the second wavelength value mapping relationship set. The second target wavelength value is used to characterize the change in the absorption capacity of the first sensing unit 412 to incident light when the adsorption functional layer 4213 adsorbs the target molecules under steady-state constraint conditions.

[0069] Among them, the incident light emitted by the light emitter 10 has the same first transmission direction in the light incident portion 411 and the transition transmission portion 413, and the incident light has the same second transmission direction in the light exit portion 415 and the transition transmission portion 413, and the first transmission direction is opposite to the second transmission direction.

[0070] Specifically, see Figure 3 , Figure 3 It is a schematic diagram of a light transmission path provided in an embodiment of the present application. The transmission path of the incident light emitted by the light emitter 10 is as follows: the incident light enters the first light transmission segment 20, passes through the first light transmission segment 20 and then enters the first port 31 of the optical fiber connector 30, comes out from the third port 33 and enters the light incident part 411 in the detection sensor segment 40, passes through the light incident part 411 and enters the first sensor part 412, passes through the first sensor part 412 and enters the transition transmission part 413, passes through the transition transmission part 413 and enters the second sensor part 414, passes through the second sensor part 414 and enters the light exit part 415, passes through the light exit part 415 and enters the second light transmission segment 50, and finally enters the signal processing device 60 through the second light transmission segment 50.

[0071] It can be seen that the first light transmission section 20 and the light incident portion 411, as well as the light exit portion 415 and the second light transmission section 50 are connected through the optical fiber connector, so as to avoid light spilling out, which is beneficial to improving the accuracy of detection.

[0072] See also Figure 4 , Figure 44 is a schematic diagram of another biological detection sensor provided in an embodiment of the present application, wherein the light incident portion 411 includes a first optical fiber 4110, wherein the first optical fiber 4110 includes a first cladding 4111 and a first core 4112, wherein the first sensing portion 412 includes a second optical fiber 4120 and a first metal film 4121 wrapped around the surface of the second optical fiber 4120, wherein the second optical fiber 4120 is connected to the first core 4112, wherein the incident light enters the second optical fiber 4120 from the first core 4112 and generates a plasma resonance reaction on the first metal film 4121; wherein the transition transmission portion 413 includes a third optical fiber 4130, wherein the third optical fiber 4130 includes a second cladding 4131 and a second core 4132, wherein the second core 4132 is connected to an end of the second optical fiber 4120 away from the first core 4112, wherein the incident light passes through the second optical fiber 4120 and the incident light passes through the second optical fiber 4120. 120 enters the second fiber core 4132; the second sensing part 414 includes a fourth optical fiber 4140 and a second metal film 4141 wrapped on the surface of the fourth optical fiber 4140, the fourth optical fiber 4140 is connected to the end of the second fiber core 4132 away from the second optical fiber 4120, the incident light enters the fourth optical fiber 4140 from the second fiber core 4132, and generates a plasma resonance reaction on the second metal film 4141; the light emitting part 415 includes a fifth optical fiber 4150, the fifth optical fiber 4150 includes a third cladding 4151 and a third fiber core 4152, the third fiber core 4152 is connected to the end of the fourth optical fiber 4140 away from the second fiber core 4132, the incident light enters the third fiber core 4152 through the fourth optical fiber 4140, and is emitted through the third fiber core 4152 to enter the signal processing device 60.

[0073] Optionally, the first metal film 4121 and the second metal film 4141 are composed of one or more of a single metal, a mixed alloy, a multi-layer metal composite layer or a semiconductor layer, and have abundant free electrons. The preparation method of the metal film includes but is not limited to depositing it on the surface of the optical fiber by thermal evaporation vacuum coating.

[0074] The first light transmission section 20 includes a sixth optical fiber connected to the first port 31 of the optical fiber connector 30 , and the second light transmission section 50 includes a seventh optical fiber connected to the second port 32 of the optical fiber connector 30 .

[0075] Among them, the first optical fiber 4110, the third optical fiber 4130, the fifth optical fiber 4150, the sixth optical fiber and the seventh optical fiber are multimode optical fibers or single-mode optical fibers; the second optical fiber 4120 and the fourth optical fiber 4140 are multimode optical fibers or single-mode optical fibers or coreless optical fibers.

[0076] Optionally, the light incident portion 411 includes a first connection area 4113, the first connection area 4113 is arranged on a side of the first cladding 4111 adjacent to the first sensing portion 412, and two ends of the first connection area 4113 are respectively connected to the second optical fiber 4120 and the first cladding 4111; the transition transmission portion 413 includes a second connection area 4133 and a third connection area 4134, the second connection area 4133 is arranged on a side of the second cladding 4131 adjacent to the first sensing portion 412, and two ends of the second connection area 4133 are respectively connected to the second optical fiber 4120 and the first cladding 4111. The second optical fiber 4120 and the second cladding 4131, the third connecting area 4134 is arranged on the side of the second cladding 4131 adjacent to the second sensing part 414, and the two ends of the third connecting area 4134 are respectively connected to the fourth optical fiber 4140 and the second cladding 4131; the light emitting part 415 includes a fourth connecting area 4153, the fourth connecting area 4153 is arranged on the side of the third cladding 4151 adjacent to the second sensing part 414, and the two ends of the fourth connecting area 4153 are respectively connected to the fourth optical fiber 4140 and the third cladding 4151.

[0077] It can be seen that by transitionally connecting the sensor part and other components through the connection area, light spillage can be avoided, which is beneficial to improving the accuracy of detection.

[0078] Among them, the temperature sensitive material is affected by the temperature and undergoes thermal expansion and contraction or other structural changes due to the temperature change, resulting in a change in the refractive index of the surface of the second metal film 4141. In one case, when the temperature rises, the refractive index of the surface of the second metal film 4141 increases, and the temperature sensitive material is affected by the temperature and undergoes thermal expansion and contraction or other structural changes due to the temperature change, resulting in a greater degree of change in the refractive index of the surface of the second metal film 4141 than the refractive index of the surface of the first metal film 4121 caused by the adsorption of target molecules. Therefore, the second wavelength segment in which the second metal film 4141 absorbs photon energy is greater than the first wavelength segment in which the first metal film 4121 absorbs photon energy, that is, any wavelength value in the second wavelength segment is greater than the wavelength value in the first wavelength segment. For the reference spectrum at this time, please refer to Figure 5A , Figure 5A is a schematic diagram of a reference spectrum provided in an embodiment of the present application, Figure 5AThe reference spectrum curve corresponding to the reference spectrum signal is shown in , the ordinate represents the intensity of the emitted light, the unit is cd candela, the abscissa represents the wavelength of light, the unit is nm, the reference spectrum curve includes two trough areas, namely the first trough area and the second trough area, the trough area is the bending transition area of ​​the target spectrum curve, the trough area is open along the increasing trend direction of the ordinate value; the wavelength corresponding to the peak of the first trough area is the third resonance peak wavelength, the wavelength corresponding to the peak of the second trough area is the first resonance peak wavelength, at this time the first resonance peak wavelength is greater than the third resonance peak wavelength. In another case, when the temperature rises, the refractive index of the surface of the second metal film 4141 will decrease, the second wavelength segment of the second metal film 4141 surface absorbing photon energy is smaller than the first wavelength segment of the first metal film 4121 surface absorbing photon energy, that is, any wavelength value in the second wavelength segment is smaller than the wavelength value in the first wavelength segment. For the reference spectrum diagram at this time, please refer to Figure 5B , Figure 5B is a schematic diagram of another reference spectrum provided in an embodiment of the present application, Figure 5B , the reference spectrum curve corresponding to the reference spectrum signal is shown in the figure, the ordinate represents the intensity of the emitted light, the unit is cd candela, the abscissa represents the wavelength of light, the unit is nm, the reference spectrum curve includes two trough areas, namely the first trough area and the second trough area, at this time, the wavelength corresponding to the peak of the first trough area is the first resonance peak wavelength, the wavelength corresponding to the peak of the second trough area is the third resonance peak wavelength, and the first resonance peak wavelength is smaller than the third resonance peak wavelength.

[0079] Similarly, the standard spectrum also corresponds to two cases. For the first case, please refer to Fig. 6A , Fig. 6A is a schematic diagram of a standard spectrum provided in an embodiment of the present application, Fig. 6A, the target spectrum curve corresponding to the target spectrum signal is shown in FIG. 1 , the ordinate represents the intensity of the emitted light, the unit is cd candela, the abscissa represents the wavelength of light, the unit is nm, the target spectrum curve includes two trough regions, namely the third trough region and the fourth trough region; the first metal film 4121 adsorbs the target molecules, resulting in a change in the surface refractive index of the first metal film 4121, when the incident light passes through the first sensing portion 412, a plasma resonance is generated on the surface of the first metal film 4121 of the first sensing portion 412, the energy of the photons in the first wavelength range of the incident light is absorbed, and the third trough region of the emitted light spectrum is formed, due to the second sensing portion 41 The temperature sensitive material on the surface of the second metal film 4141 of 4 expands and contracts or undergoes other structural changes due to the influence of temperature, resulting in a larger refractive index on the surface of the second metal film 4141. When the incident light passes through the second sensing portion 414, a plasma resonance is generated on the surface of the second metal film 4141, which absorbs the energy of the photons in the second wavelength range of the incident light, forming a fourth trough region of the spectrum of the emitted light. At this time, the wavelength value corresponding to the peak of the third trough region is the fourth resonance peak wavelength, the wavelength value corresponding to the peak of the fourth trough region is the second resonance peak wavelength, and the second resonance peak wavelength is greater than the fourth resonance peak wavelength; and the peak of the third trough region is greater than the fourth resonance peak wavelength. Figure 5A The peak of the first trough area in the graph moves to the right, and the peak of the fourth trough area is Figure 5A The peak of the second valley region in also moves to the right. For the second case, see Figure 6B , Figure 6B is a schematic diagram of another standard spectrum diagram provided in an embodiment of the present application, Figure 6B , the target spectrum curve corresponding to the target spectrum signal is shown in the figure, the ordinate represents the intensity of the emitted light, the unit is cd candela, the abscissa represents the wavelength of light, the unit is nm, and the target spectrum curve includes two trough areas, namely the third trough area and the fourth trough area; because the temperature-sensitive material on the surface of the second metal film 4141 of the second sensing portion 414 undergoes thermal expansion and contraction or other structural changes due to the influence of temperature, the refractive index of the surface of the second metal film 4141 becomes smaller, at this time, the wavelength value corresponding to the peak of the third trough area is the second resonance peak wavelength, the wavelength value corresponding to the peak of the fourth trough area is the fourth resonance peak wavelength, and the second resonance peak wavelength is smaller than the fourth resonance peak wavelength; and the peak of the fourth trough area is smaller than the Figure 5B The peak of the second trough area in the graph moves to the right, and the peak of the third trough area is Figure 5B The peak of the first trough area in the graph moves to the left.

[0080] Among them, the second target wavelength value is determined by the third resonance peak wavelength and the fourth resonance peak wavelength, which eliminates the interference of the background liquid concentration and is beneficial to improving the accuracy of detection. The first target wavelength value is determined by the first resonance peak wavelength and the second resonance peak wavelength, which can realize the detection of the temperature of the target detection solution while detecting the target molecule concentration, which is beneficial to improving the detection efficiency.

[0081] The first resonance peak wavelength is greater than the third resonance peak wavelength, and the second resonance peak wavelength is greater than the fourth resonance peak wavelength; or the first resonance peak wavelength is less than the third resonance peak wavelength, and the second resonance peak wavelength is less than the fourth resonance peak wavelength.

[0082] It can be seen that in this example, by passing the incident light of the light emitter 10 through the first sensing part 412 and the second sensing part 414 in sequence, the temperature of the target detection solution can be detected while also detecting the concentration of the target molecules in the target detection solution, which is beneficial to improving the detection efficiency. At the same time, it can also eliminate the interference of the background liquid concentration, which is beneficial to improving the accuracy of the detection.

[0083] In a possible example, the biosensing detection system 1 is a single-channel system, that is, the biosensing detection system 1 is only provided with a single biosensing sensor 41 .

[0084] In a possible example, the biosensor detection system 1 is a multi-channel system, that is, the biosensor detection system 1 is provided with a plurality of biosensor sensors 41 for simultaneous detection.

[0085] See also Figure 7 , Figure 7 1 is a schematic diagram of another biosensor detection system provided in an embodiment of the present application. The biosensor detection system 1 comprises a light emitter 10 , a biodetection channel 70 , a transmission switch 80 and a signal processing device 60 .

[0086] Optionally, there may be multiple biological detection channels 70, which is not limited here.

[0087] The transmission switch 80 may be an optical path switch.

[0088] Among them, a single biological detection channel 70 includes a first light transmission segment 20, a fiber optic connector 30, a detection sensor segment 40 and a second light transmission segment 50. The second light transmission segment 50 in each biological detection channel 70 is connected to a transmission switch 80, and the transmission switch 80 is connected to a signal processing device 60. The first light transmission segment 20 is connected to the detection sensor segment 40 through the fiber optic connector 30, and the second light transmission segment 50 is connected to the detection sensor segment 40 through the fiber optic connector 30.

[0089] The transmission switch 80 is used to switch the biological detection channel 70 in time-sharing mode, so as to ensure that the detection signal of only one biological detection channel 70 is transmitted to the signal processing device 60 at the same time.

[0090] It can be understood that in other embodiments of the present application, the optical fiber connector 30 is integrated into an optical fiber connector instrument.

[0091] Optionally, a single or multiple light emitters 10 are provided in the biosensor detection system 1, and a single light emitter 10 can transmit light to one or more first light transmission segments 20, which is not limited in the present application.

[0092] It can be seen that the multi-channel system can detect multiple solutions to be detected at the same time, which is beneficial to improving the detection efficiency.

[0093] See also Figure 8 , Figure 8 It is a structural diagram of another biosensor detection system provided in an embodiment of the present application. The biosensor detection system 1 further includes an interaction instrument 90 , and the interaction instrument 90 is connected to the signal processing device 60 .

[0094] Among them, the interaction instrument 90 is used to obtain the first target characterization value and the second target characterization value from the signal processing device 60, and analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule based on the first target characterization value and the second target characterization value.

[0095] See also Fig. 9 , Fig. 9 This is a flow chart of a biosensor detection method provided by an embodiment of the present application, which is applied to a signal processing device in a biosensor detection system, wherein the biosensor detection system includes a light emitter, a first light transmission segment, an optical fiber adapter, a second light transmission segment, a detection sensing segment and the signal processing device, wherein the detection sensing segment includes a biodetection sensor, and the biodetection sensor includes a first sensing part and a second sensing part, wherein the first light transmission segment is connected to a first port of the optical fiber adapter, one end of the second light transmission segment is connected to a second port of the optical fiber adapter, and the two ends of the biodetection sensor are respectively connected to a third port and a fourth port of the optical fiber adapter, wherein a first metal film of the first sensing part is modified with an adsorption functional layer, and a second metal film of the second sensing part is modified with a temperature sensitive layer, and the signal processing device is connected to the second light transmission segment; the method includes:

[0096] S901, obtaining a reference spectral signal of the first outgoing light from the biological detection sensor, and obtaining a standard spectral signal of the second outgoing light from the biological detection sensor, wherein the reference spectral signal is a detection signal of the biological detection sensor in a reference detection solution, and the standard spectral signal is a detection signal of the biological detection sensor in the target detection solution and when the adsorption functional layer adsorbs the target molecules in the target detection solution in a steady state and under other conditions.

[0097] S902, determining a first resonance peak wavelength and a third resonance peak wavelength according to the reference spectrum signal;

[0098] S903, determining a second resonance peak wavelength and a fourth resonance peak wavelength according to the standard spectrum signal;

[0099] S904, determining a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determining a second target wavelength value according to the third resonance peak wavelength and the fourth resonance peak wavelength, wherein the first target wavelength value is used to characterize a change in the absorption capacity of the biodetection sensor to the incident light of the light emitter under the temperature constraint of the target detection solution, and the second target wavelength value is used to characterize a change in the absorption capacity of the biodetection sensor to the incident light under the condition of steady-state constraint when the adsorption functional layer adsorbs the target molecule;

[0100] S905, determining the temperature of the target detection solution according to the first target wavelength value and the first wavelength value mapping relationship set, and determining the concentration of the target molecule in the target detection solution according to the second target wavelength value and the second wavelength value mapping relationship set.

[0101] It can be seen that in this example, the signal processing device can detect the temperature of the target detection solution while also detecting the concentration of the target molecules in the target detection solution, which is beneficial to improving the detection efficiency. It can also eliminate the interference of the background liquid concentration, which is beneficial to improving the detection accuracy.

[0102] In one possible example, the interaction instrument is connected to the signal processing device, and the interaction instrument is used to obtain the first target characterization value and the second target characterization value from the signal processing device, and analyze the strength of the interaction affinity between the target molecule and the target targeting object of the target molecule based on the first target characterization value and the second target characterization value.

[0103] In one possible example, the signal processing device is used to determine the third resonance peak wavelength of the reference spectral signal, determine the fourth resonance peak wavelength of the standard spectral signal, determine a second target wavelength value based on the third resonance peak wavelength and the fourth resonance peak wavelength, and determine the concentration of the target molecule in the target detection solution based on a set of mapping relationships between the second target wavelength value and the second wavelength value, wherein the second target wavelength value is used to characterize the change in the absorption capacity of the biological detection sensor to the incident light under steady-state constraints when the adsorption functional layer adsorbs the target molecule.

[0104] In a possible example, the first resonance peak wavelength is greater than the third resonance peak wavelength, the second resonance peak wavelength is greater than the fourth resonance peak wavelength, or,

[0105] The first resonance peak wavelength is shorter than the third resonance peak wavelength, and the second resonance peak wavelength is shorter than the fourth resonance peak wavelength.

[0106] In one possible example, the temperature sensitive layer includes a temperature sensitive material, and the temperature sensitive material includes at least one of the following: polydimethylsiloxane, indium tin oxide, silver, and liquid crystal; the temperature sensitive layer is used to isolate the detection solution of the second sensing part; the adsorption functional layer includes a sealing agent and a target target of the target molecule, and the sealing agent is used to seal the area on the first metal film where the target target is not modified.

[0107] In a possible example, the first target wavelength value is the relative difference between the first resonance peak wavelength and the second resonance peak wavelength or the numerical value of the division operation result; the second target wavelength value is the relative difference between the third resonance peak wavelength and the fourth resonance peak wavelength or the numerical value of the division operation result; the first wavelength value mapping relationship set includes the corresponding relationship between the first wavelength value and the temperature of the target detection solution, and the absolute value of the first wavelength value is positively correlated with the temperature of the target detection solution; the second wavelength value mapping relationship set includes the corresponding relationship between the second wavelength value and the concentration of the target molecule in the target detection solution, and the second wavelength value is positively correlated with the concentration of the target molecule.

[0108] In a possible example, the transition transmission portion is bent, the light incident portion and the light emitting portion are symmetrically arranged, and the first sensing portion and the second sensing portion are symmetrically arranged.

[0109] In a possible example, the incident light has the same first transmission direction in the light incident portion and the transition transmission portion, and the incident light has the same second transmission direction in the light exit portion and the transition transmission portion, and the first transmission direction and the second transmission direction are opposite.

[0110] In one possible example, the light incident portion includes a first optical fiber, which includes a first cladding and a first core, and one end of the first core is connected to the third port of the optical fiber connector; the first sensing portion includes a second optical fiber and the first metal film wrapped on the surface of the second optical fiber, and one end of the second optical fiber is connected to the other end of the first core; the transition transmission portion includes a third optical fiber, which includes a second cladding and a second core, and one end of the second core is connected to the other end of the second optical fiber; the second sensing portion includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, and one end of the fourth optical fiber is connected to the other end of the second core; the light emitting portion includes a fifth optical fiber, which includes a third cladding and a third core, one end of the third core is connected to the other end of the fourth optical fiber, and the other end of the third core is connected to the fourth port of the optical fiber connector.

[0111] The spectral detector provided in this embodiment is used to execute the above-mentioned biosensing detection method, and thus can achieve the same effect as the above-mentioned implementation method.

[0112] In the case of an integrated unit, the spectrum detector may include a processing module, a storage module and a communication module. The processing module may be used to control and manage the actions of the spectrum detector, for example, it may be used to support the spectrum detector to execute the steps performed by the above functional unit. The storage module may be used to support the spectrum detector to execute stored program codes and data, etc. The communication module may be used to support the communication between the spectrum detector and other devices.

[0113] Among them, the processing module can be a processor or a controller. It can implement or execute various exemplary logic boxes, modules and circuits described in conjunction with the disclosure of this application. The processor can also be a combination that implements computing functions, such as a combination of one or more microprocessors, a combination of digital signal processing (DSP) and a microprocessor, etc. The storage module can be a memory. The communication module can specifically be a device that interacts with other electronic devices, such as a radio frequency circuit, a Bluetooth chip, a Wi-Fi chip, etc.

[0114] An embodiment of the present application also provides a computer storage medium, wherein the computer storage medium stores a computer program for electronic data exchange, wherein the computer program enables a computer to execute part or all of the steps of any method recorded in the above method embodiments, and the above computer includes a spectral detector.

[0115] The present application also provides a computer program product, which includes a non-transitory computer-readable storage medium storing a computer program, and the computer program is operable to cause a computer to execute some or all of the steps of any method described in the method embodiment. The computer program product may be a software installation package, and the computer includes a control platform.

[0116] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present application is not limited by the described order of actions, because according to the present application, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present application.

[0117] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0118] In the several embodiments provided in the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are only schematic, such as the division of the above-mentioned units, which is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical or other forms.

[0119] The units described above as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0120] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.

[0121] If the above-mentioned integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable memory. Based on this understanding, the technical solution of the present application, or the part that contributes to the prior art, or all or part of the technical solution can be embodied in the form of a software product. The computer software product is stored in a memory, including a number of instructions to enable a computer device (which can be a personal computer, server or network device, etc.) to execute all or part of the steps of the above-mentioned methods in each embodiment of the present application. The aforementioned memory includes: U disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), mobile hard disk, disk or optical disk and other media that can store program codes.

[0122] A person of ordinary skill in the art can understand that all or part of the steps in the various methods of the above embodiments can be completed by instructing related hardware through a program, and the program can be stored in a computer-readable memory, which can include: a flash drive, a read-only memory, a random access memory, a magnetic disk or an optical disk, etc.

[0123] The embodiments of the present application are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. At the same time, for general technical personnel in this field, according to the idea of ​​the present application, there will be changes in the specific implementation method and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A biosensor detection system, characterized in that: The biosensor detection system comprises: A light transmitter; a first light transmission segment; a fiber optic connector, wherein the first light transmission segment is connected to a first port of the fiber optic connector; a second light transmission segment, wherein one end of the second light transmission segment is connected to a second port of the fiber optic connector; A detection sensing section, wherein the detection sensing section comprises a biological detection sensor, and the biological detection sensor comprises a light incident part, a first sensing part, a transition transmission part, a second sensing part and a light emitting part; two ends of the light incident part are respectively connected to the third port of the optical fiber connector and one end of the first sensing part, two ends of the light emitting part are respectively connected to the fourth port of the optical fiber connector and one end of the second sensing part, and two ends of the transition transmission part are respectively connected to the other end of the first sensing part and the other end of the second sensing part; the first light transmission section is connected to the light incident part through the first port and the third port, and the second light transmission section is connected to the light emitting part through the second port and the fourth port; the first metal film of the first sensing part is modified with an adsorption functional layer, and the second metal film of the second sensing part is modified with a temperature sensitive layer; a signal processing device, the signal processing device being connected to the other end of the second light transmission section; the signal processing device being used to collect a reference spectrum signal of the first outgoing light from the biological detection sensor, collect a standard spectrum signal of the second outgoing light from the biological detection sensor, determine a first resonance peak wavelength of the reference spectrum signal and a second resonance peak wavelength of the standard spectrum signal, determine a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine a temperature of a target detection solution according to a set of mapping relationships between the first target wavelength value and the first wavelength value, the reference spectrum signal being a detection signal of the biological detection sensor in the reference detection solution, the standard spectrum signal being a detection signal of the biological detection sensor in the target detection solution and under the constraints of a steady state and temperature conditions and background liquid concentration conditions when the adsorption functional layer adsorbs target molecules in the target detection solution, the first target wavelength value being used to characterize a change in the absorption capacity of the biological detection sensor for the incident light of the light emitter under the constraints of the temperature of the target detection solution; The signal processing device is also used to determine the third resonance peak wavelength of the reference spectrum signal, determine the fourth resonance peak wavelength of the standard spectrum signal, determine the second target wavelength value according to the third resonance peak wavelength and the fourth resonance peak wavelength, and determine the concentration of the target molecule in the target detection solution according to the second target wavelength value and the second wavelength value mapping relationship set, the second target wavelength value is used to characterize the change in the absorption capacity of the biosensor to the incident light under steady-state constraint conditions when the adsorption functional layer adsorbs the target molecule; The adsorption functional layer includes a sealing agent and a target target of the target molecule, and the sealing agent is used to seal the area on the first metal film that is not modified with the target target; Wherein, the reference spectrum curve includes two trough areas, namely, a first trough area and a second trough area, the wavelength corresponding to the peak of the first trough area is the third resonance peak wavelength, the wavelength corresponding to the peak of the second trough area is the first resonance peak wavelength, and the first resonance peak wavelength is greater than the third resonance peak wavelength, the standard spectrum curve includes two trough areas, namely, a third trough area and a fourth trough area, the wavelength value corresponding to the peak of the third trough area is the fourth resonance peak wavelength, the wavelength value corresponding to the peak of the fourth trough area is the second resonance peak wavelength, and the second resonance peak wavelength is greater than the fourth resonance peak wavelength, or, The wavelength corresponding to the peak of the first wave valley region is the first resonance peak wavelength, the wavelength corresponding to the peak of the second wave valley region is the third resonance peak wavelength, the first resonance peak wavelength is smaller than the third resonance peak wavelength, the wavelength value corresponding to the peak of the third wave valley region is the second resonance peak wavelength, the wavelength value corresponding to the peak of the fourth wave valley region is the fourth resonance peak wavelength, and the second resonance peak wavelength is smaller than the fourth resonance peak wavelength.

2. The system according to claim 1, characterized in that The first metal film and the second metal film are composed of one or more of a single metal, a mixed alloy, a multi-layer metal composite layer or a semiconductor layer.

3. The system according to claim 1, characterized in that The temperature sensitive layer includes a temperature sensitive material, and the temperature sensitive material includes at least one of the following: polydimethylsiloxane, indium tin oxide, silver, and liquid crystal; the temperature sensitive layer is used for the second sensing part to isolate the detection solution.

4. The system according to claim 1, characterized in that The first target wavelength value is a relative difference value or a division result value between the first resonance peak wavelength and the second resonance peak wavelength; The second target wavelength value is a relative difference between the third resonance peak wavelength and the fourth resonance peak wavelength or a division result value; The first wavelength value mapping relationship set includes a corresponding relationship between the first wavelength value and the temperature of the target detection solution, and the absolute value of the first wavelength value is positively correlated with the temperature of the target detection solution; The second wavelength value mapping relationship set includes a corresponding relationship between the second wavelength value and the concentration of the target molecule in the target detection solution, and the second wavelength value is positively correlated with the concentration of the target molecule.

5. The system according to claim 1, characterized in that The transition transmission portion is bent, the light incident portion and the light emitting portion are symmetrically arranged, and the first sensing portion and the second sensing portion are symmetrically arranged.

6. The system according to claim 5, characterized in that The incident light has the same first transmission direction in the light incident portion and the transition transmission portion, and the incident light has the same second transmission direction in the light exit portion and the transition transmission portion, and the first transmission direction is opposite to the second transmission direction.

7. The system according to claim 5, characterized in that The light incident portion includes a first optical fiber, the first optical fiber includes a first cladding and a first core, and one end of the first core is connected to the third port of the optical fiber connector; The first sensing part includes a second optical fiber and the first metal film wrapped on the surface of the second optical fiber, and one end of the second optical fiber is connected to the other end of the first fiber core; The transition transmission part includes a third optical fiber, the third optical fiber includes a second cladding and a second core, and one end of the second core is connected to the other end of the second optical fiber; The second sensing part includes a fourth optical fiber and the second metal film wrapped on the surface of the fourth optical fiber, and one end of the fourth optical fiber is connected to the other end of the second fiber core; The light emitting portion includes a fifth optical fiber, the fifth optical fiber includes a third cladding and a third core, one end of the third core is connected to the other end of the fourth optical fiber, and the other end of the third core is connected to the fourth port of the optical fiber connector.

8. A biosensor detection method, characterized in that: Applied to the biosensor detection system of claim 1, the method comprising: Acquire a reference spectral signal of the first outgoing light from the biodetection sensor, and acquire a standard spectral signal of the second outgoing light from the biodetection sensor, wherein the reference spectral signal is a detection signal of the biodetection sensor in a reference detection solution, and the standard spectral signal is a detection signal of the biodetection sensor in the target detection solution and when the adsorption functional layer adsorbs the target molecule in the target detection solution in a steady state and under the constraints of temperature conditions and background liquid concentration conditions; Determine a first resonance peak wavelength and a third resonance peak wavelength according to the reference spectrum signal; Determine the second resonance peak wavelength and the fourth resonance peak wavelength according to the standard spectrum signal; Determine a first target wavelength value according to the first resonance peak wavelength and the second resonance peak wavelength, and determine a second target wavelength value according to the third resonance peak wavelength and the fourth resonance peak wavelength, wherein the first target wavelength value is used to characterize the change in the absorption capacity of the biosensor to the incident light of the light emitter under the temperature constraint of the target detection solution, and the second target wavelength value is used to characterize the change in the absorption capacity of the biosensor to the incident light under the condition of steady-state constraint when the adsorption functional layer adsorbs the target molecule; The temperature of the target detection solution is determined according to the first target wavelength value and the first wavelength value mapping relationship set, and the concentration of the target molecule in the target detection solution is determined according to the second target wavelength value and the second wavelength value mapping relationship set.

9. An interaction instrument, characterized in that: The interaction instrument comprises the biosensor detection system according to any one of claims 1 to 7, and the interaction instrument is used to analyze the strength of the interaction affinity between a target molecule and a target target of the target molecule.

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