A convenient stem cell resuscitation device

Abstract

The present invention relates to the technical field of stem cell resuscitation, and discloses a conveniently operated stem cell resuscitation device, including a cell resuscitation box, wherein a mobile transport mechanism is arranged inside the cell resuscitation box for automatically driving a cryopreservation tube containing cells for resuscitation, a bath pot resuscitation mechanism is arranged above the mobile transport mechanism for clamping the cryopreservation tube and shaking it in hot water, a disinfection wiping mechanism is arranged on one side of the mobile transport mechanism for disinfecting and drying the cell resuscitation box, a placement clamp at one end is pushed out of an opening of the cell resuscitation box by a U-shaped slide, and the placement clamp is moved while the door is opened, so that space inside the cell resuscitation box can be saved, so that the staff can directly place the cryopreservation tube while opening the door, thereby reducing steps and improving the convenience of operation, and the staff only needs to place the cryopreservation tube, and the equipment can automatically resuscitate after closing the box door, and the sample can be protected from external pollution.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell resuscitation, and in particular to a conveniently controlled stem cell resuscitation device. Background Art

[0002] Simply put, stem cells are a type of cell with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated daughter cells. The definition of stem cells has been revised over the years and defined at different levels. Most biologists and medical scientists believe that stem cells are a type of cell from embryos, fetuses or adults that has unlimited self-renewal and proliferation and differentiation capabilities under certain conditions, and can produce daughter cells with the same phenotype and genotype as itself, as well as specialized cells that make up the body's tissues and organs, and can also differentiate into progenitor cells.

[0003] The operator wears sterile protective clothing and sterile gloves, takes the frozen stem cells out of the storage container, puts them in a 37°C water bath or preheated buffered saline solution, thaws them quickly, takes the thawed cells out of the packaging solution, gently blows with a pipette or pipette to remove the residual packaging solution, centrifuges the cell suspension in a centrifuge at an appropriate speed for several minutes, removes the supernatant, resuspends the cells in an appropriate sterile culture medium, and adjusts the cell concentration to the required density.

[0004] When the above-mentioned stem cell resuscitation device is in use, the staff uses tweezers to hold the lid of the cryotube and places it in a water bath for thawing. Since the stem cells in the cryotube need to be placed in 37°C hot water to slowly thaw, the staff needs to use tweezers to hold the cryotube. Maintaining the same posture for a long time will cause fatigue of the wrist, forearm and shoulder muscles, affecting subsequent experimental operations. After thawing, the staff needs to wipe the surface of the cryotube with an alcohol cloth. If the hands are not wiped clean, bacteria, viruses or other contaminants are easily transferred to the sample in the cryotube. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides an easy-to-operate stem cell resuscitation device with the advantages of convenient operation, etc., which solves the problems of slow thawing efficiency of manually clamping cryopreservation tubes and easy cell damage caused by manual wiping.

[0006] In order to achieve the above purpose of not only solving the slow thawing efficiency of manually clamping cryopreservation tubes, but also ensuring the improvement of stem cell sliding efficiency, the present invention provides the following technical solutions: a conveniently operated stem cell resuscitation device, comprising a cell resuscitation box, a mobile transport mechanism is arranged in the cell resuscitation box, which is used to automatically drive the cryopreservation tubes containing cells for resuscitation, a bath pot resuscitation mechanism is arranged above the mobile transport mechanism, which is used to clamp the cryopreservation tubes and shake them in hot water, a disinfection wiping mechanism is arranged on one side of the mobile transport mechanism, which is used to disinfect and dry the cell resuscitation box, the mobile transport mechanism comprises a box door rotatably connected to the cell resuscitation box, a transmission rod is rotatably connected to one side of the box door, a slidable sliding bar is rotatably connected to one end of the transmission rod away from the box door, a limited position sliding groove is provided on the cell resuscitation box, a U-shaped slide is connected to the upper surface of the sliding bar, a placement clamp is arranged at one end of the U-shaped slide, a cryopreservation tube is clamped on the placement clamp, a slidable sliding door is connected to one side of the U-shaped slide, and an extraction groove is provided on one side of the cell resuscitation box.

[0007] As a preferred solution of the convenient-to-operate stem cell resuscitation device described in the present invention, one end of the U-shaped slide is connected to an interrupted rack, an interrupted groove is provided on the interrupted rack, the upper surface of the interrupted rack is connected to a fixed frame, and a reciprocating screw rod is rotatably connected to the fixed frame.

[0008] As a preferred solution of the conveniently operated stem cell resuscitation device of the present invention, a through movable slide groove is provided on the fixed frame, a sliding column is slidably connected to the fixed frame, and an L sliding frame is connected to the lower surface of the sliding column.

[0009] As a preferred solution of the convenient-to-operate stem cell resuscitation device of the present invention, an adjusting slider is slidably connected to the L sliding frame, a placement clamp is connected to one side of the adjusting slider, and an adjusting slot is provided on one side of the L sliding frame.

[0010] As a preferred solution of the conveniently controlled stem cell resuscitation device of the present invention, one end of the reciprocating screw rod is connected to a circular cover, ratchets are connected inside the circular cover, and a rotation hole is opened on one side of the circular cover.

[0011] As a preferred solution of the conveniently operated stem cell resuscitation device of the present invention, a T-shaped round block is rotatably connected in the annular cover, a rotating disk is connected to one side of the T-shaped round block, and rotating sliders are connected to torsion springs at both ends of the rotating disk.

[0012] As a preferred solution of the easy-to-operate stem cell resuscitation device described in the present invention, a driving gear is connected to the side of the rotating disk away from the T-shaped round block, a rack plate is meshed on the lower surface of the driving gear, and the rack plate is connected to one side of a cell resuscitation box, and a water bath is connected to the cell resuscitation box.

[0013] As a preferred solution of the convenient-to-operate stem cell resuscitation device described in the present invention, a temperature display for real-time temperature measurement is installed on one side of the water bath, a beveled slider is connected inside the water bath, a water inlet pipe is connected to the other side of the water bath, and a drain pipe is connected to the lower surface of the water bath.

[0014] As a preferred solution of the easy-to-operate stem cell resuscitation device described in the present invention, a V-shaped limit plate is connected to the water bath, one side of the cell resuscitation box is rotatably connected to a driven gear 1, the other side of the cell resuscitation box is rotatably connected to a driven gear 2, the lower surface of the driven gear 2 is connected to a cleaning roller, the lower surface of the cleaning roller is connected to an extrusion disk, and the lower surface of the extrusion disk is connected to an arc-shaped pressure block.

[0015] As a preferred solution of the easy-to-operate stem cell resuscitation device described in the present invention, the lower surface of the arc-shaped pressure block is slidably connected to a storage box, a suction tube is connected to the storage box, one end of the suction tube is connected to an air bag, the end of the air bag away from the suction tube is connected to a delivery tube, the end of the delivery tube away from the air bag is connected to an injection stand, and the injection stand is connected to an atomizing nozzle.

[0016] Compared with the known prior art, the technical solution provided by the present invention has the following beneficial effects:

[0017] 1. Hold the handle on the door and drive the door to rotate and open along the shaft on one side of the cell recovery box. When the transmission rod moves, it will drive the sliding bar to slide along the limit slide groove in the cell recovery box through the other end. The U-shaped slide will push the placement clip at one end to extend out from the opening of the cell recovery box. Move the placement clip while opening the door, which can save space inside the cell recovery box, so that the staff can directly place the cryopreserved tube while opening the door, reducing the steps and improving the convenience of operation. The staff only needs to place the cryopreserved tube, and the equipment can automatically recover after closing the door, which can protect the sample from external contamination.

[0018] 2. When the cryopreservation tube is driven to slide into the water bath by the placement clamp, the cryopreservation tube clamped by the placement clamp will slide down along the adjustment slide groove into the water bath under the action of its own weight. When the cryopreservation tube slides along the water bath to the beveled slider at the other end, the cryopreservation tube will gradually drive the placement clamp to move upward along the adjustment slide groove, so that the cryopreservation tube is removed from the hot water in the water bath to complete thawing. The staff can be freed from tedious manual operations and focus on other important tasks, which improves job satisfaction and reduces the workload of operators. Especially for situations where a large number of samples need to be processed, the risk of sample damage or contamination caused by inaccurate manual operations is reduced, making the sample processing process more efficient;

[0019] 3. The driving gear rotates in the opposite direction along the meshing rack plate below. The rotating disk on one side of the driving gear will drive the rotating sliders at both ends to rotate in the annular cover and contact the vertical surface of the ratchet teeth. The reciprocating screw will drive the sliding column to slide back and forth along the moving slide groove on the fixed frame, which will drive the cryopreservation tube placed on the clamp to shake in the hot water in the water bath. By shaking the cryopreservation tube, the hot water can evenly contact every part of the cryopreservation tube, thereby ensuring that the inside and outside of the sample are effectively thawed at the same time, preventing local overheating or overcooling, reducing cell or tissue damage caused by uneven temperature, and helping to quickly increase the sample temperature and accelerate the thawing speed.

[0020] 4. The interrupted rack will simultaneously drive the meshing driven gears 1 and 2 on both sides to rotate, so that the cleaning rollers on the lower surfaces of the driven gears 1 and 2 will rotate, and the cleaning rollers on both sides can wrap and clean the cryotubes. At the same time, the rotation of the extrusion plate will drive the arc-shaped pressing block to squeeze the air bag on the upper surface of the storage box. The alcohol is intermittently sprayed onto the rotating cleaning roller through the atomizing nozzle, which can disinfect and wipe the surface of the cryotube. The use of the alcohol cleaning roller for wiping can utilize the cooling effect of alcohol during evaporation, and reduce the large temperature difference when the cryotube is just taken out of hot water. Rapid and appropriate disinfection can protect the sample from harmful substances in the environment. By controlling the spraying frequency, the amount of alcohol used can be saved, thereby reducing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0023] Figure 2 This is a schematic diagram of the structure of the cell recovery box of the present invention;

[0024] Figure 3 It is a structural schematic diagram of the mobile transport mechanism of the present invention;

[0025] Figure 4 This is a schematic diagram of the structure of the interrupted rack of the present invention;

[0026] Figure 5 It is a structural schematic diagram of the hot spring resuscitation mechanism of the present invention;

[0027] Figure 6 It is a structural schematic diagram of the rotating disk of the present invention;

[0028] Figure 7 It is a structural schematic diagram of an enlarged portion of the bath pot resuscitation mechanism A of the present invention;

[0029] Figure 8 It is a structural schematic diagram of the disinfection wiping mechanism of the present invention;

[0030] Fig. 9 It is a schematic diagram of the structure of the extrusion disk of the present invention.

[0031] In the figure: 100, cell recovery box; 200, mobile transport mechanism; 201, box door; 202, transmission rod; 203, sliding bar; 204, limiting slide; 205, U-shaped slide; 206, placement clamp; 207, cryopreservation tube; 208, sliding door; 209, extraction tank; 300, bath recovery mechanism; 301, interrupted rack; 302, interrupted slot; 303, fixed frame; 304, reciprocating screw rod; 305, mobile slide; 306, sliding column; 307, L slide frame; 308, adjustment slider; 309, adjustment slide; 310, annular cover; 311, ratchet; 312 , rotating hole; 313, T-shaped round block; 314, rotating disk; 315, rotating slider; 316, driving gear; 317, rack plate; 318, water bath; 319, temperature display; 320, bevel slider; 321, water inlet pipe; 322, drain pipe; 400, disinfection wiping mechanism; 401, V-shaped limit plate; 402, driven gear one; 403, driven gear two; 404, cleaning roller; 405, extrusion disk; 406, arc-shaped pressure block; 407, storage box; 408, suction tube; 409, air bag; 410, delivery tube; 411, injection stand; 412, atomizing nozzle. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The present invention will be further described below in conjunction with the embodiments.

[0034] Example 1

[0035] Reference Figure 1-3, which is the first embodiment of the present invention, provides a conveniently operated stem cell resuscitation device, including a cell resuscitation box 100, wherein a mobile transport mechanism 200 is provided in the cell resuscitation box 100, for automatically driving a cryopreservation tube 207 containing cells for resuscitation, a bath pot resuscitation mechanism 300 is provided above the mobile transport mechanism 200, for clamping the cryopreservation tube 207 and shaking it in hot water, and a disinfection wiping mechanism 400 is provided on one side of the mobile transport mechanism 200, for disinfecting and drying the cell resuscitation box 100;

[0036] The mobile transport mechanism 200 includes a box door 201 rotatably connected to the cell recovery box 100, a transmission rod 202 is rotatably connected to one side of the box door 201, and a slidable sliding bar 203 is rotatably connected to the end of the transmission rod 202 away from the box door 201. A limited position slide groove 204 is provided on the cell recovery box 100, and a U-shaped slide 205 is connected to the upper surface of the sliding bar 203. A placement clamp 206 is provided at one end of the U-shaped slide 205, and a cryopreservation tube 207 is clamped on the placement clamp 206. A slidable A sliding door 208, an extraction tank 209 is provided on one side of the cell recovery box 100, and a transmission rod 202 is rotatably connected to the end of the box door 201 away from the rotating shaft, similar to a crank disc. The shape of the sliding bar 203 matches the limiting sliding groove 204 provided in the cell recovery box 100, and a U-shaped slide 205 is fixedly connected to the upper surface of the sliding bar 203. The shape of the U-shaped slide 205 is an inverted U-shape, which can avoid collision with other mechanisms during sliding. The U-shaped slide 205 is fixedly connected to the sliding door 208 on one side of the extraction tank 209 corresponding to the U-shaped slide 205.

[0037] During use, the staff member holds the handle on the box door 201 to drive the box door 201 to rotate and open along the shaft on one side of the cell recovery box 100. During the rotation of the box door 201, the transmission rod 202 on the other side is driven to move. While the transmission rod 202 moves, it drives the sliding bar 203 to slide along the limiting slide groove 204 in the cell recovery box 100 through the other end. The U-shaped slide 205 pushes the placement clip 206 at one end to extend out from the opening of the cell recovery box 100. At the same time, the U-shaped slide 205 The sliding door 208 on the side of the cell recovery box 100 will slide along the inner wall of the cell recovery box 100 to cover and close the extraction tank 209. The staff will take out the cryopreservation tube 207 from the dry ice box and place it on the placement clamp 206 for clamping and fixing. The water temperature can be checked through the observation window on the side of the cell recovery box 100. The staff will drive the box door 201 to rotate in the opposite direction and close it by using the handle. At the same time, the placement clamp 206 extending out of the cell recovery box 100 will drive the cryopreservation tube 207 to move into the cell recovery box 100 for thawing.

[0038] Example 2

[0039] Reference Figure 1-7, which is the second embodiment of the present invention, provides a stem cell resuscitation device that is easy to operate, one end of the U-shaped slide 205 is connected to an interrupted rack 301, the interrupted rack 301 is provided with an interrupted groove 302, the upper surface of the interrupted rack 301 is connected to a fixed frame 303, the fixed frame 303 is rotatably connected to a reciprocating screw rod 304, one end of the U-shaped slide 205 is fixedly connected to the interrupted rack 301, the upper surface of the interrupted rack 301 is fixedly connected to the fixed frame 303, the same number of teeth are arranged on both sides of the interrupted rack 301, and the interrupted groove 302 is provided in the middle of two teeth.

[0040] A through movable groove 305 is provided on the fixed frame 303, and a sliding column 306 is slidably connected to the fixed frame 303. The lower surface of the sliding column 306 is connected to an L sliding frame 307. The interruption groove 302 provided on the interruption rack 301 is aligned with the movable groove 305 provided on the upper fixed frame 303. The sliding column 306 drives the L sliding frame 307 below to slide left and right on the fixed frame 303 along the movable groove 305 and the interruption groove 302. A limiting ring is fixedly connected to the sliding column 306 to prevent the sliding column 306 from falling off the fixed frame 303.

[0041] An adjusting slider 308 is slidably connected to the L sliding frame 307, one side of the adjusting slider 308 is connected to the placement clamp 206, one side of the L sliding frame 307 is provided with an adjusting slot 309, the adjusting slot 309 provided on the L sliding frame 307 is slidably matched with the shape of the adjusting slider 308, one side of the adjusting slider 308 is fixedly connected to the placement clamp 206, and the placement clamp 206 can slide up and down on one side of the L sliding frame 307 along the adjusting slot 309 through the adjusting slider 308.

[0042] One end of the reciprocating screw rod 304 is connected to a circular cover 310, and a ratchet 311 is connected inside the circular cover 310. A rotating hole 312 is opened on one side of the circular cover 310. The shape of the circular cover 310 is basin-shaped, and a circle of ratchet teeth 311 are fixedly connected to the inner wall of the circular cover 310 at intervals. The shape of the ratchet teeth 311 is arc-shaped on one side and a vertical plane on the other side.

[0043] A T-shaped round block 313 is rotatably connected inside the annular cover 310, and a rotating disk 314 is connected to one side of the T-shaped round block 313. Rotating sliders 315 are connected to torsion springs at both ends of the rotating disk 314. The T-shaped round block 313 is rotatably connected in the rotating hole 312. The shapes of the T-shaped round block 313 and the rotating hole 312 match. The rotating disk 314 drives the rotating sliders 315 at both ends in the annular cover 310. The shape of the rotating slider 315 is a convex arc on one side and a concave arc on the other side. When the rotating slider 315 is in the initial position of the torsion spring, it can rotate toward the concave side, and cannot continue to rotate toward the convex side, and will be blocked by the fixed column.

[0044] A driving gear 316 is connected to the side of the rotating disk 314 away from the T-shaped round block 313, and a rack plate 317 is meshed on the lower surface of the driving gear 316. The rack plate 317 is connected to one side of the cell recovery box 100. A water bath 318 is connected to the cell recovery box 100. One side of the driving gear 316 is fixedly connected to the rotating disk 314, and the water bath 318 is filled with hot water heated to 37°C by a heater.

[0045] A temperature display meter 319 capable of real-time temperature measurement is installed on one side of the water bath 318, a beveled slider 320 is connected inside the water bath 318, a water inlet pipe 321 is connected to the other side of the water bath 318, a drain pipe 322 is connected to the lower surface of the water bath 318, a beveled slider 320 is fixedly connected to the side of the water bath 318 away from the door 201, and the beveled slider 320 is in the shape of an acute triangle, and water control valves are installed on the water inlet pipe 321 and the drain pipe 322 connected to the water bath 318.

[0046] During use, when the box door 201 is rotated and opened along one side of the cell recovery box 100, the U-shaped slide 205 will push the interrupted rack 301 at one end to move toward the opening of the cell recovery box 100, and the interrupted rack 301 will drive the fixed frame 303 with the reciprocating screw 304 on the upper surface to move together, and the driving gear 316 set at one end of the reciprocating screw 304 will mesh with the rack plate 317 along one side of the cell recovery box 100 to rotate. When the driving gear 316 rotates, it will drive the rotating disk 314 on one side to rotate in the annular cover 310, and the rotation of the torsion springs at both ends of the rotating disk 314 The slider 315 will slide along the arc surface of the ratchet 311 and cannot drive the reciprocating screw 304 on the other side of the annular cover 310 to rotate on the fixed frame 303. When the box door 201 is rotated in the opposite direction to close, the U-shaped slide 205 will pull the interrupted rack 301 at one end to slide into the cell recovery box 100. At this time, the driving gear 316 will rotate in the opposite direction along the meshing rack plate 317 below. The rotating disk 314 on one side of the driving gear 316 will drive the rotating sliders 315 at both ends to rotate in the annular cover 310 and contact and resist with the vertical surface of the ratchet 311, so that the annular cover 310 drives The reciprocating screw rod 304 on one side rotates on the fixed frame 303, and a sliding column 306 is slidably connected in the slide rail on the reciprocating screw rod 304. When the reciprocating screw rod 304 rotates, it will drive the sliding column 306 to slide back and forth along the movable slide groove 305 on the fixed frame 303. When the placement clamp 206 drives the cryopreservation tube 207 to slide into the water bath 318, the cryopreservation tube 207 clamped by the placement clamp 206 will slide down along the adjustment slide groove 309 into the water bath 318 under the action of its own weight. When the sliding column 306 slides, it will drive the cryopreservation tube 207 on the placement clamp 206 to slide in the water bath 318. 8, when the cryopreservation tube 207 slides along the water bath 318 to the beveled slider 320 at the other end, the cryopreservation tube 207 will gradually drive the placement clamp 206 to move upward along the adjustment slide groove 309, so that the cryopreservation tube 207 is removed from the hot water in the water bath 318 to complete thawing. The real-time temperature of the hot water in the water bath 318 can be accurately observed through the temperature display meter 319. When the hot water in the water bath 318 needs to be replaced, the water in the water bath 318 can be drained through the drain pipe 322, and then water can be re-injected into the water bath 318 through the water inlet pipe 321 for heating.

[0047] The remaining structure is the same as that of Example 1.

[0048] Example 3

[0049] Reference Figure 1-9, which is the third embodiment of the present invention, provides a stem cell resuscitation device that is easy to operate, a V-shaped limit plate 401 is connected to the water bath 318, one side of the cell resuscitation box 100 is rotatably connected to a driven gear 1 402, and the other side of the cell resuscitation box 100 is rotatably connected to a driven gear 2 403, the lower surface of the driven gear 2 403 is connected to a cleaning roller 404, the lower surface of the cleaning roller 404 is connected to a squeezing disk 405, and the lower surface of the squeezing disk 405 is connected to an arc-shaped pressing block 406, the driven gear 1 402 and the driven gear 2 403 are respectively rotatably connected to the top surface of the cell resuscitation box 100, and the driven gear 1 402 and the driven gear 2 403 are both installed with cleaning rollers 404 and squeezing disks 405, the cleaning rollers 404 are made of fluffy cleaning cloth, and the cleaning rollers 404 on both sides can evenly wrap the cryopreservation tubes 207 passing through.

[0050] The lower surface of the arc-shaped pressing block 406 is slidably connected to a storage box 407, a suction tube 408 is connected inside the storage box 407, one end of the suction tube 408 is connected to an air bag 409, the end of the air bag 409 away from the suction tube 408 is connected to a delivery tube 410, the end of the delivery tube 410 away from the air bag 409 is connected to an injection stand 411, and the injection stand 411 is connected to an atomizing nozzle 412. The rotation of the extrusion disk 405 drives the arc-shaped pressing block 406 on the lower surface to be squeezed along the surface of the air bag 409. The arc-shaped pressing block 406 and the air bag 409 are both arc-shaped, and a one-way valve is arranged in the suction tube 408 to prevent alcohol from flowing back into the storage box 407.

[0051] During use, the placement clamp 206 will drive the cryopreservation tube 207 to slide to the middle along the V-shaped limit plates 401 on both sides. When the interrupted rack 301 passes between the driven gear 1 402 and the driven gear 2 403 along the top surface of the cell recovery box 100, the interrupted rack 301 will simultaneously drive the meshed driven gear 1 402 and the driven gear 2 403 on both sides to rotate, so that the cleaning rollers 404 on the lower surfaces of the driven gear 1 402 and the driven gear 2 403 rotate, and the placement clamp 206 will drive the cryopreservation tube 207 to pass between the cleaning rollers 404 on both sides. The cleaning rollers 404 on both sides can wrap and clean the cryopreservation tube 207, and the cleaning rollers 404 will drive the lower end extrusion disk 405 to rotate while rotating. The rotation of 405 will drive the arc-shaped pressing block 406 to squeeze the airbag 409 on the upper surface of the storage box 407. A one-way valve is arranged in the suction tube 408. The alcohol stored in the airbag 409 will be transported to the injection stand 411 along the delivery tube 410, and sprayed onto the rotating cleaning roller 404 through the atomizing nozzle 412, so as to disinfect and wipe the surface of the cryopreservation tube 207. When the arc-shaped pressing block 406 passes through the airbag 409, the compressed airbag 409 will extract the alcohol into the airbag 409 through the suction tube 408 under the action of the pressure difference for storage. When the box door 201 is completely closed, the sliding door 208 will slide along the inner wall and the extraction groove 209 will be opened. The placement clamp 206 will drive the cryopreservation tube 207 to appear at the extraction groove 209.

[0052] The remaining structure is the same as that of Example 1.

[0053] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A conveniently operated stem cell resuscitation device, comprising a cell resuscitation box (100), characterized in that: The cell resuscitation box (100) is provided with a mobile transport mechanism (200) for automatically driving a cryopreservation tube (207) containing cells for resuscitation; a bath resuscitation mechanism (300) is provided above the mobile transport mechanism (200) for clamping the cryopreservation tube (207) and shaking it in hot water; and a disinfection wiping mechanism (400) is provided on one side of the mobile transport mechanism (200) for disinfecting and drying the cell resuscitation box (100); The mobile transport mechanism (200) comprises a box door (201) rotatably connected to the cell recovery box (100); a transmission rod (202) is rotatably connected to one side of the box door (201); an end of the transmission rod (202) away from the box door (201) is rotatably connected to a slidable sliding bar (203); a limited position sliding groove (204) is provided on the cell recovery box (100); a U-shaped sliding frame (205) is connected to the upper surface of the sliding bar (203); a placement clamp (206) is provided at one end of the U-shaped sliding frame (205); a cryotube (207) is clamped on the placement clamp (206); a slidable sliding door (208) is connected to one side of the U-shaped sliding frame (205); an extraction tank (209) is provided on one side of the cell recovery box (100); and a water bath (318) is connected inside the cell recovery box (100); The water bath (318) is connected to a V-shaped limit plate (401); one side of the cell recovery box (100) is rotatably connected to a driven gear 1 (402); the other side of the cell recovery box (100) is rotatably connected to a driven gear 2 (403); a cleaning roller (404) is connected to the lower surface of the driven gear 2 (403); a pressing plate (405) is connected to the lower surface of the cleaning roller (404); and a curved pressing block (406) is connected to the lower surface of the pressing plate (405). ), the lower surface of the arc-shaped pressing block (406) is slidably connected to a storage box (407), a suction tube (408) is connected inside the storage box (407), one end of the suction tube (408) is connected to an air bag (409), an end of the air bag (409) away from the suction tube (408) is connected to a delivery tube (410), an end of the delivery tube (410) away from the air bag (409) is connected to an injection stand (411), and an atomizing nozzle (412) is connected to the injection stand (411); One end of the U-shaped slide (205) is connected to an interrupted rack (301), an interrupted groove (302) is provided on the interrupted rack (301), an upper surface of the interrupted rack (301) is connected to a fixed frame (303), and a reciprocating screw rod (304) is rotatably connected to the fixed frame (303).

2. The conveniently controlled stem cell resuscitation device according to claim 1, characterized in that: The fixed frame (303) is provided with a through movable sliding groove (305), the fixed frame (303) is slidably connected with a sliding column (306), and the lower surface of the sliding column (306) is connected with an L sliding frame (307).

3. The conveniently controlled stem cell resuscitation device according to claim 2, characterized in that: An adjusting slider (308) is slidably connected to the L sliding frame (307), one side of the adjusting slider (308) is connected to a placement clamp (206), and one side of the L sliding frame (307) is provided with an adjusting sliding groove (309).

4. The conveniently controlled stem cell resuscitation device according to claim 3, characterized in that: One end of the reciprocating screw rod (304) is connected to a circular ring cover (310), a ratchet tooth (311) is connected inside the circular ring cover (310), and a rotation hole (312) is opened on one side of the circular ring cover (310).

5. The conveniently controlled stem cell resuscitation device according to claim 4, characterized in that: A T-shaped round block (313) is rotatably connected inside the annular cover (310), one side of the T-shaped round block (313) is connected to a rotating disk (314), and torsion springs at both ends of the rotating disk (314) are connected to rotating sliders (315).

6. The conveniently controlled stem cell resuscitation device according to claim 5, characterized in that: A driving gear (316) is connected to the side of the rotating disk (314) away from the T-shaped circular block (313); a rack plate (317) is meshed on the lower surface of the driving gear (316); and the rack plate (317) is connected to one side of the cell recovery box (100).

7. The conveniently controlled stem cell resuscitation device according to claim 6, characterized in that: A temperature display (319) capable of real-time temperature measurement is installed on one side of the water bath (318); a beveled slider (320) is connected inside the water bath (318); a water inlet pipe (321) is connected to the other side of the water bath (318); and a drain pipe (322) is connected to the lower surface of the water bath (318).

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