A plant salt-tolerant growth-promoting bacterium JP2-21 and its application

Abstract

The present application relates to the field of microbial technology, and specifically discloses a plant salt-tolerant growth-promoting bacterium JP2-21 and its application; the plant salt-tolerant growth-promoting bacterium JP2-21 was deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration on October 24, 2023, with a deposit number of CGMCC No.28731, and is classified and named Bacillus atrophaeus ( Bacillus atrophaeus ); The growth-promoting bacteria JP2‑21 of the present application can efficiently produce auxin, and its IAA production capacity is 16.19 mg / L. It has the ability to solubilize phosphorus, fix nitrogen, and chelate iron, and can effectively promote the growth of tomatoes, as well as improve the salt tolerance of tomatoes. The growth-promoting bacteria JP2‑21 of the present application also has disease resistance, and can significantly inhibit various pathogens such as pear gray mold, pear ring pattern, apple rot, poplar rot, pepper phytophthora, and wolfberry root rot, reduce the spread of diseases, and has broad application potential.

Description

Technical Field

[0001] The present application relates to the field of microbial technology, and more specifically, to a plant salt-tolerant growth-promoting bacterium JP2-21 and its application. Background Art

[0002] As a valuable natural resource, soil provides the material basis for human survival and is also the earliest means of production used by humans. However, with the extensive development of resources by humans, soil salinization, as one of the main types of land degradation, is common in most arid and semi-arid areas in the world, becoming one of the important factors affecting sustainable agricultural development, environmental safety and economic development. The accumulated soluble salts in the soil solution will lead to a reduction in grain production, or even a complete failure in severe cases, thus posing a threat to food security. There are a large number of undeveloped salinized soils in China and even around the world. Calculated at the current population growth rate and food supply level, the global food supply needs to increase by more than 57% by 2050. Therefore, alleviating soil salinization to ensure food security has become a hot issue of concern to countries around the world today.

[0003] Bacteria that grow in the rhizosphere of plants and are beneficial to plant growth are called plant growth promoting rhizobacteria (PGPR). PGPR can prevent and control pathogens in the soil, reduce the harm of pathogens to plants, improve the absorption and utilization efficiency of mineral nutrients by plants, produce metabolites that are beneficial to plant growth, thereby promoting plant growth and increasing crop yields, and can also improve the ability of plants to resist biological and abiotic stresses. Using plant rhizosphere promoting bacteria and organic fertilizers to replace or supplement chemical fertilizers is an important direction for the development of modern agriculture to reduce soil degradation and salinization. After inoculation with salt-tolerant growth-promoting bacteria, plants promote plant growth, improve plant tolerance to salt stress, and thus enhance the ability of plants to survive in adversity. Xu Fangfang et al. found that under 100mM NaCl stress, wheat inoculated with multiple strains of Enterobacteriaceae isolated from the rhizosphere of desert plants can significantly promote wheat growth. Hungria et al. showed that Azospirillum can significantly increase the yield of soybeans and beans under salt stress. The Achromobacterpiechaudii strain isolated by Mayak et al. can significantly increase the fresh weight and dry weight of tomato seedlings under 172 mM NaCl stress. Bacilio et al.'s study showed that Azospirillumlipoferum can colonize wheat roots and promote wheat growth under salt stress.

[0004] Suaeda salsa belongs to an annual herbaceous fleshy halophyte with high salt tolerance. It can grow well between 100mM and 300mM NaCl concentrations, and the highest salt tolerance concentration can reach 600mM NaCl, or even 800mM NaCl. The plant is widely distributed in Asia and also distributed in Europe. Suaeda salsa is widely distributed in China, and is distributed in Northeast China, Inner Mongolia Autonomous Region, Hebei Province, Shanxi Province, Shaanxi Province, Ningxia Hui Autonomous Region, Gansu Province, Qinghai Province, Xinjiang Uygur Autonomous Region, Shandong Province, Jiangsu Province and Zhejiang Province. It mainly grows in saline-alkali soils, beaches and lakeside areas, and often forms a single species community. Therefore, using Suaeda salsa as a model plant to study Suaeda salsa rhizosphere microorganisms will be of great significance for promoting crop growth and improving the soil conditions of crops. Based on the above statement, the present application provides a plant salt-tolerant growth-promoting bacterium JP2-21 and its application. Summary of the invention

[0005] In order to solve the above technical problems, the present application provides a plant salt-tolerant growth-promoting bacterium JP2-21 and its application.

[0006] In the first aspect, the present application provides a plant salt-tolerant growth-promoting bacterium JP2-21, which adopts the following technical solution:

[0007] A plant salt-tolerant growth-promoting bacterium JP2-21, which was deposited on October 24, 2023 at the General Microbiology Center of the China Culture Collection Administration, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 28731 and a classification name of Bacillus atrophaeus.

[0008] Preferably, the 16S rRNA nucleotide sequence of the plant salt-tolerant growth-promoting bacteria JP2-21 is as shown in SEQ ID NO.1.

[0009] In the second aspect, the present application provides a method for isolating and culturing plant salt-tolerant growth-promoting bacteria JP2-21, using the following technical solution:

[0010] A method for isolating and culturing plant salt-tolerant growth-promoting bacteria JP2-21 comprises the following steps:

[0011] S1. Soil samples were collected from the rhizosphere of Suaeda salsa in Inner Mongolia Autonomous Region, and the bacteria in the soil samples were isolated. Then, single colonies with obvious differences in colony morphology were selected for purification and culture on LB medium. The isolated bacteria were screened using Montgina organophosphate medium, Ashby nitrogen fixation medium, and CAS iron-degrading medium to obtain strain JP2-21.

[0012] S2. Pick a single colony of strain JP2-21 and inoculate it into LB liquid culture medium, shake and culture it on a shaker at a temperature of 28-32°C and a rotation speed of 160-200r / min for 32-48h to obtain seed liquid, inoculate the seed liquid into LB liquid culture medium with an inoculum amount of 1-3% by volume, place it on a shaker at a temperature of 28-32°C and a rotation speed of 160-200r / min for 32-48h to obtain fermentation liquid.

[0013] Preferably, the culture temperature is 30°C, the culture speed is 180r / min, and the culture time is 32h.

[0014] Preferably, the seed solution inoculation amount is 2% of the LB liquid culture medium by volume.

[0015] In the third aspect, the present application provides an application of plant salt-tolerant growth-promoting bacteria JP2-21, using the following technical solution:

[0016] The use of the plant salt-tolerant growth-promoting bacteria JP2-21 described in the first aspect in the preparation of a growth-promoting bacterial agent.

[0017] The use of the plant salt-tolerant growth-promoting bacteria JP2-21 described in the first aspect above in promoting tomato growth and salt-tolerant products.

[0018] In a fourth aspect, the present application provides a growth-promoting bacterial agent, which adopts the following technical solution:

[0019] A growth-promoting bacterial agent comprises the plant salt-tolerant growth-promoting bacteria JP2-21 described in the first aspect.

[0020] Preferably, the active ingredient of the growth-promoting bacteria is live bacteria, fermentation liquid or bacterial suspension of plant salt-tolerant growth-promoting bacteria JP2-21.

[0021] Preferably, the growth-promoting bacteria agent is in the form of a wettable powder, a water dispersible agent, a water suspension or a dispersible oil suspension.

[0022] In a fifth aspect, the present application provides the use of the above-mentioned plant salt-tolerant growth-promoting bacteria JP2-21 in the preparation of a product for inhibiting plant pathogens, using the following technical solution:

[0023] An application of the plant salt-tolerant growth-promoting bacteria JP2-21 in the preparation of a product for inhibiting plant pathogens.

[0024] Preferably, the plant pathogenic bacteria are Botryosphaeria cinerea, Botryosphaeria berengerianade, Cytospora chrysosperma YSFL4-2, Cytospora mali QH2, Phytophthora capsici SP90, and Fusarium sambucinum GQGF1-3.

[0025] In summary, this application has the following beneficial effects:

[0026] The plant salt-tolerant growth-promoting bacteria JP2-21 disclosed in the present application can efficiently produce auxin, and its ability to produce auxin (IAA) is 16.19 mg / L. It has the ability to solubilize phosphorus, fix nitrogen, and chelate iron. It can effectively promote the growth of tomatoes and improve the salt tolerance of tomatoes. It has a wide range of applications and has certain application potential.

[0027] The growth-promoting bacteria JP2-21 of the present application also has disease resistance effects, and can significantly inhibit various pathogens such as pear gray mold, pear ring pattern, apple rot, poplar rot, pepper phytophthora, and wolfberry root rot, reduce the spread of diseases, and has broad application potential.

[0028] This application isolates rhizosphere microorganisms from the rhizosphere of Suaeda salsa, screens their growth-promoting effects, and combines the screened strains with dominant horticultural crops such as tomatoes for potted plant experiments to further verify the growth-promoting and salt-tolerant effects of the strains. Screen out strains with excellent performance, enrich the market strain resources, and provide data support for the construction of efficient and widely applicable growth-promoting agents. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a diagram showing the results of the in-dish screening of the strain JP2-21 in Example 1 of the present application for decomposing organic phosphorus, fixing nitrogen, chelating iron and producing auxin;

[0030] Figure 2 This is a graph showing the effect of strain JP2-21 in Example 2 of the present application on the salt tolerance of potted tomatoes;

[0031] Figure 3 This is a diagram showing the promoting effect of the treatment of strain JP2-21 in Example 2 of the present application on the salt tolerance of potted tomatoes;

[0032] Figure 4 This is a graph showing the in-dish antagonistic activity of strain JP2-21 against six diseases in Example 3 of the present application;

[0033] Figure 5 This is a graph showing the in vivo activity of strain JP2-21 against four diseases in Example 3 of the present application;

[0034] Figure 6 The plate culture characteristics and scanning electron microscope morphology of strain JP2-21 in Example 4 of the present application;

[0035] Figure 7 A phylogenetic tree was constructed for the 16S rDNA sequence of strain JP2-21 in Example 4 of the present application. DETAILED DESCRIPTION

[0036] The present invention is further described in detail below in conjunction with the accompanying drawings and specific examples of the specification. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0037] The pathogens involved in the examples of this application are as follows:

[0038] Pear gray mold (Botryosphaeria cinerea), pear ring rot (Botryosphaeria berengerianade), poplar rot (Cytospora chrysosperma YSFL4-2), apple rot (Cytospora mali QH2), wolfberry root rot (Fusarium sambucinum GQGF1-3), pepper phytophthora (Phytophthora capsici SP90).

[0039] The culture medium and reagents involved in the examples of this application are as follows:

[0040] (1) LB liquid medium: 10.0 g of peptone, 5.0 g of yeast powder, 2.0 g of sodium chloride, 1000 mL of distilled water, pH 7.0 (solid medium is based on the liquid medium plus 18.0 g of agar powder).

[0041] (2) Montana organophosphate medium (1 L): glucose 10.0 g, NaCl 0.3 g, KCl 0.3 g, FeSO4·7H2O0.03 g, (NH4)2SO4 0.5 g, MnSO4·7H2O 0.03 g, MgSO4·7H2O 0.3 g, lecithin 0.2 g, CaCO3 5 g, yeast extract 0.4 g, distilled water 1000 ml, pH 7.0-7.5.

[0042] (3) Ashby nitrogen-fixing medium (1 L): KH2PO4 0.2 g, NaCl 0.2 g, MgSO4·7H2O0.2 g, K2SO4·2H2O 0.2 g, CaCO3 5.0 g, glucose 5.0 g, mannitol 5.0 g, pH 7.0.

[0043] (4) CAS iron-dissolving medium (mg / L): 68.5% chrome azurol S (CAS), 72.9% hexadecyltrimethylammonium bromide (HDTMA), 2.645% ferric chloride hexahydrate, 295.25% sodium dihydrogen phosphate dihydrate, 1213.5% disodium hydrogen phosphate dodecahydrate, 125% ammonium chloride, 37.5% potassium dihydrogen phosphate, 62.5% sodium chloride, 9000% agar, pH 6.8±0.1. When preparing, weigh 10.87 g, heat to boiling and completely dissolve it in 1L distilled water, sterilize at 120°C for 30 min and set aside.

[0044] (5) Salkowski colorimetric solution for IAA production determination: 15 mL 0.5 mol / L FeCl3, 300 mL concentrated sulfuric acid, 500 mL distilled water, mix before use and store in the dark. When using, add 4 mL of Salkowski colorimetric solution to 1 mL of sample.

[0045] Example 1

[0046] Isolation of strain JP2-21 and determination of its growth-promoting ability

[0047] Soil samples were collected from the rhizosphere of Suaeda salsa in Inner Mongolia Autonomous Region. When collecting soil samples, 5 cm of the ground surface was removed and soil samples within 25 cm near the roots were taken. The collected soil was mixed evenly and sealed in a kraft paper bag in a refrigerator at 4°C. The bacteria in the soil samples were separated by the stepwise dilution method, and then single colonies with obvious differences in colony morphology were selected for purification and culture on LB liquid medium.

[0048] The isolated strains were screened using Montgena organic phosphorus medium, Ashby nitrogen fixation medium, and CAS iron-degrading medium;

[0049] The ability of the strain to demineralize elements was evaluated by measuring the transparent zone (D), colony diameter (d), and calculating D / d;

[0050] At the same time, the strain's ability to produce tryptophan (IAA) was determined: the isolated strain was inoculated in LB liquid culture medium containing 100 mg / L L-tryptophan, and cultured at 28°C and 180 r / min for 2 days. Then, 200 μL of the bacterial suspension was transferred to a 48-well plate, and 800 μL of Salkowski colorimetric solution was added. 200 μL of uninoculated LB liquid culture medium and 800 μL of Salkowski colorimetric solution were used as controls. After being placed at room temperature and away from light for 20 minutes, the color turned pink for positive, indicating that it can secrete IAA. The darker the color, the greater the secretion intensity.

[0051] Quantitative determination of the IAA production capacity of the strain: the strain was inoculated in LB liquid medium containing 500 mg / L L-tryptophan, and its OD was measured by L-tryptophan. 600 nm OD value, take 5mL in a 10mL centrifuge tube, centrifuge at 10000r / min for 10min, take 1mL of the supernatant, add 4mL of Salkowski colorimetric solution, place in the dark at room temperature for 20min, and measure the OD value. 530 The OD value at nm was used to find the corresponding IAA content on the standard curve.

[0052] Table 1 The results of in-dish screening of phosphate solubilization, nitrogen fixation and iron complexation of strain JP2-21

[0053]

[0054] Combination Figure 1 The results in Table 1 show that the strain JP2-21 with the ability to solubilize phosphorus, fix nitrogen, complex iron and produce galactin was isolated from the rhizosphere of Suaeda salsa, and its IAA production capacity was 16.19 mg / L. Figure 1 A) Nitrogen fixation (such as Figure 1 B), complex iron (such as Figure 1 C) and IAA production (such as Figure 1 D).

[0055] Example 2

[0056] Detection of the salt-tolerant growth-promoting ability of strain JP2-21 on tomato

[0057] (1) Preparation of bacterial suspension: JP2-21 was inoculated into LB liquid medium and cultured on a shaker at 28°C and 180 r / min for 48 h. After the strain was fully grown, the OD of the suspension of each strain was measured using a spectrophotometer. 600 and adjust OD with sterile water 600 =1.0.

[0058] (2) Potted treatment: Tomato varieties with excellent performance were sown in seedling pots mixed with fine sand and vermiculite in a ratio of 1:2. After the seeds broke through the soil and germinated, 50 mL of bacterial solution was added to each pot. The control group (CK) was irrigated with an equal amount of LB liquid culture medium, which were set as follows: CK without NaCl (referred to as CK 0%), CK + 0.5% NaCl (referred to as CK 0.5%), CK + 1% NaCl (referred to as CK 1%), CK + 1.5% NaCl (referred to as CK 1.5%), and JP2-21 without NaCl (referred to as JP2-21 0%), JP2-21 + 0.5% NaCl (referred to as JP2-21 0.5%), JP2-21 + 1% NaCl (referred to as JP2-21 1%), JP2-21 + 1.5% NaCl (referred to as JP2-21 1.5%), the second inoculation was carried out 10 days after the first inoculation, and the tomato plants were harvested after they grew for 30 days.

[0059] (3) Measurement indicators: After the plants are harvested, their height and stem thickness are measured, and they are washed and rinsed before being measured using a root scanner to measure the root growth (root length, root area, root diameter, root volume, number of root tips and number of forks).

[0060] Combination Figure 2 and Figure 3 The results showed that strain JP2-21 could significantly improve the growth of tomatoes. Tomatoes in the control group showed symptoms such as lodging and dwarfing, but tomatoes treated with JP2-21 grew well and basically did not lodging. Compared with the control group, strain JP2-21 significantly improved tomato plant height, stem thickness, and root growth, and could effectively improve the salt tolerance of tomatoes.

[0061] Example 3

[0062] Determination of anti-disease activity of strain JP2-21

[0063] (1) Antagonistic activity assay of strain JP2-21 in vitro

[0064] The antagonistic effect of strain JP2-21 against pear gray mold (Botryosphaeria cinerea), pear ring rot (Botryosphaeria berengerianade), poplar rot pathogen (Cytosporachrysosperma YSFL4-2), apple rot pathogen (Cytospora mali QH2), wolfberry root rot pathogen (Fusarium sambucinum GQGF1-3) and pepper phytophthora capsici SP90 was determined by using the plate confrontation method in a dish and the live inoculation method.

[0065] The inoculation needle picked up JP2-21 with a diameter of 6 mm and placed it on both sides of the PDA plate. After culturing at 28°C for 2 days, the six pathogenic bacteria cakes to be tested were inoculated in the center of the plate respectively, and the treatment of inoculating pathogenic bacteria cakes only in the center of the plate was used as the control. Each treatment was repeated three times and cultured at a constant temperature of 28°C. When the control colony filled the plate, the diameter of the inhibition zone was observed and recorded, and the inhibition rate was calculated.

[0066] (2) The live control effect of strain JP2-21 on pear gray mold and pear filamentous wilt

[0067] Soak healthy and fresh Huangguan pears in 2% sodium hypochlorite for 3 minutes, rinse with tap water and dry. Use a sterile punch to make a 5mm (diameter) × 3mm (depth) wound at the equator of the pear fruit, spray the antagonistic bacteria solution on the wound, and stick the two pathogen cakes on the wound and fix them with tape after 0.5 hours. Use the Huangguan pear sprayed with sterile water and inoculated with pathogens only as the control. Each treatment is repeated 6 times, and the treated fruits are sealed with plastic wrap. Place in an artificial climate box, 25℃, 16h light / 8h dark, moisturizing and culture for 7 days, and measure the diameter of the lesion.

[0068] (3) Antagonistic activity of strain JP2-21 against poplar rot and apple rot

[0069] Two-year-old healthy poplar and apple branches were selected, cut into 20-30cm, soaked in 2% sodium hypochlorite for 7 minutes, rinsed three times with sterile water and dried, sealed at both ends with paraffin, and a 6mm wound was made in the middle of the branch with a sterile puncher. The antagonistic bacteria solution was evenly sprayed on the branches. After 30 minutes, poplar rot pathogens and apple rot pathogens were inoculated respectively, wrapped with sterile absorbent cotton (soaked with sterile water), and sealed with plastic wrap. The branches sprayed with sterile water and inoculated with rot pathogen cakes after wounding were used as positive controls, and the branches not inoculated with rot pathogen cakes were used as negative controls. All branches were cultured at 25°C, 16h light / 8h dark moisture preservation. The cotton was removed 5d after inoculation, and the diameter of the lesions was measured 14d later to calculate the inhibition rate.

[0070] The antagonistic activity of strain JP2-21 against pear gray mold, pear ring rot, poplar rot, apple rot, wolfberry root rot and pepper phytophthora was calculated using control effect (%) = (1-treated lesion diameter / control lesion diameter) × 100%.

[0071] Table 2 Results of the test on the protective effect of strain JP2-21 against 6 pathogens

[0072]

[0073]

[0074] Combination Figure 4 , Figure 5The results in Table 2 show that strain JP2-21 has a significant inhibitory effect on the mycelial growth of the six tested pathogens, with an in vitro inhibition rate of more than 47% and an in vivo inhibition rate of more than 30%. The in vivo inhibition activity against poplar rot and apple rot reached more than 76%.

[0075] Figure 4 Among them, A is Botryosphaeria cinerea, B is Botryosphaeria berengerianade, C is Cytospora chrysosperma YSFL4-2, D is Cytospora mali QH2, E is Phytophthora capsici SP90, and F is Fusarium sambucinum GQGF1-3.

[0076] Figure 5 In the figure, A is Botryosphaeria cinerea, of which the upper part is the control and the lower part is the treatment with JP2-21; B is Botryosphaeria berengerianade, of which the upper part is the control and the lower part is the treatment with JP2-21; C and D are Cytospora chrysosperma YSFL4-2, of which C is the control and D is the treatment with JP2-21; E and F are Cytospora mali QH2, of which E is the control and F is the treatment with JP2-21.

[0077] Example 4

[0078] Identification of strain JP2-21

[0079] (1) Morphological identification

[0080] The purified strain JP2-21 was inoculated into LB solid medium and LB solid medium containing 7%, 10% and 12% NaCl, and the morphological characteristics of the colonies in the dish were observed. The colonies were picked and the bacterial morphology was observed under a scanning electron microscope. The strain JP2-21 appeared as milky white opaque colonies on the LB solid medium, with a flat and dry surface, smooth edges and fast growth. The bacteria were short rods with a length of 1.26-1.71μm and a width of 0.46-0.76μm under microscope. The strain can still grow vigorously on a 12% concentration of NaCl medium and has a strong salt tolerance. The results are as follows Figure 6 As shown; Among them, the back colony characteristics of strain JP2-21 on LB solid medium (such as Figure 6A), positive colony characteristics of strain JP2-21 on LB solid medium (such as Figure 6 B); SEM observation of bacterial morphology of strain JP2-21 (such as Figure 6 C); positive colony characteristics of strain JP2-21 on LB solid medium containing 7% NaCl (such as Figure 6 D); positive colony characteristics of strain JP2-21 on LB solid medium containing 10% NaCl (such as Figure 6 E); positive colony characteristics of strain JP2-21 on LB solid medium containing 12% NaCl (such as Figure 6 F).

[0081] (2) Molecular identification

[0082] The genomic DNA of strain JP2-21 was extracted and 16S rRNA was amplified by PCR. The sequencing results of the amplified products were submitted to NCBI, and similarity analysis was performed with the known 16S rRNA gene sequences in Gen Bank by BLAST to find similar species; the corresponding model strain was downloaded from the GGDC website (http: / / ggdc.dsmz.de / .), and the evolutionary tree was constructed using the neighbor method of MEGA11.

[0083] The 16S rRNA nucleotide sequence of the plant salt-tolerant growth-promoting bacteria JP2-21 is shown in SEQ ID NO.1:

[0084]

[0085] Combination Figure 7 The results showed that the homology between strain JP2-21 and Bacillus atrophaeus reached 99.79%. Strain JP2-21 and multiple strains of Bacillus atrophaeus were clustered on one branch. The morphological, physiological and biochemical characteristics combined with the molecular sequence confirmed that it was Bacillus atrophaeus.

[0086] This application will obtain the plant salt-tolerant growth-promoting bacteria JP2-21 deposited on October 24, 2023 at the General Microbiology Center of the China Culture Collection Administration, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 28731 and the classification name Bacillus atrophaeus.

[0087] The plant salt-tolerant growth-promoting bacteria JP2-21 disclosed in the present application can efficiently produce auxin, and its ability to produce auxin (IAA) is 16.19 mg / L. It has the ability to solubilize phosphorus, fix nitrogen, and chelate iron, and can effectively promote the growth of tomatoes and improve the salt tolerance of tomatoes. The growth-promoting bacteria JP2-21 of the present application also has disease resistance, and can significantly inhibit various pathogens such as pear gray mold, pear ring pattern, apple rot, poplar rot, pepper phytophthora, wolfberry root rot, etc., reduce the spread of diseases, and is widely used and has certain application potential.

[0088] This specific embodiment is only an explanation of the present application, and it is not a limitation of the present application. After reading this specification, those skilled in the art can make non-creative modifications to the present embodiment as needed, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A plant salt-tolerant growth-promoting bacterium JP2-21, characterized in that: The plant salt-tolerant growth-promoting bacteria JP2-21 was deposited on October 24, 2023 at the General Microbiology Center of the China Culture Collection Administration, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC No. 28731 and the classification name Bacillus atrophaeus.

2. Use of the plant salt-tolerant growth-promoting bacteria JP2-21 described in claim 1 in the preparation of a bacterial agent for promoting tomato growth.

3. A growth-promoting agent, characterized in that: Including the plant salt-tolerant growth-promoting bacteria JP2-21 described in claim 1.

4. The growth-promoting bacterial agent according to claim 3, characterized in that The active ingredient of the growth-promoting bacteria agent is live bacteria, fermentation liquid or bacterial suspension of plant salt-tolerant growth-promoting bacteria JP2-21.

5. The growth-promoting bacterial agent according to claim 3, characterized in that The dosage form of the growth-promoting bacteria agent is a water dispersant, a water suspension or a dispersible oil suspension.

6. Use of the plant salt-tolerant growth-promoting bacteria JP2-21 according to claim 1 in the preparation of a product for inhibiting plant pathogens; The plant pathogenic bacteria is Botryosphaeria cinerea, Botryosphaeria berengerianade, Cytospora chrysosperma, Cytospora mali, Phytophthora capsici or Fusariumsambucinum.

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