Cold resistance test method and centrifuge for black wolfberry branches

Abstract

The present invention belongs to the technical field of agricultural planting, and in particular to a cold resistance test method for black wolfberry branches and a centrifuge thereof. The present invention provides a cold resistance test method for black wolfberry branches, comprising: S1: sampling, taking branches with a diameter of about 0.5-0.6 cm and uniform thickness, pruning and sealing; S2: pre-treating the sample, cleaning the stains outside the sample and refrigerating for standby use; S3: low-temperature treatment of the sample, placing the sample in a low-temperature program-controlled container, processing the temperature gradient setting, and maintaining the low temperature for a certain period of time, and then heating the sample; S4: obtaining a supernatant, adding an extract to the sample quantitatively, grinding it into a homogenate, placing the homogenate in a centrifuge for centrifugal separation, and taking a supernatant; S5: determining the MDA content of the supernatant.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and specifically relates to a device or method for testing rhizomes, and in particular to a cold resistance testing method for black wolfberry branches and a centrifuge thereof. Background Art

[0002] In China, black wolfberry is mainly distributed in the high-altitude, arid and semi-arid areas of northwest China. The purple-black fruit is rich in natural anthocyanins and other effective active substances. Due to its strong stress resistance and high nutritional value, it has become an emerging excellent desert shrub species with economic and ecological value in recent years. However, due to the single planting variety of black wolfberry, the yield quality of wild growth cannot be guaranteed, and there are still many difficulties in artificial cultivation.

[0003] At present, most of the planting methods are wild seed seedling raising and softwood cuttings. These planting methods have low germination rates, low seedling survival rates and low numbers of strong seedlings, and the fruit setting rate and fruit quality of mature plants cannot be guaranteed. Therefore, it is urgent to conduct extensive and in-depth research on seed source screening and propagation of excellent varieties to obtain excellent cultivated varieties and expand their suitable range.

[0004] Research has shown that low temperature is the most significant abiotic stress factor that causes freezing of plant intercellular spaces in cold climates, ultimately leading to plant death. Therefore, breeding cold-resistant varieties is one of the effective ways to reduce plant frost damage and expand its growth range.

[0005] In the related technologies, when plants are subjected to low temperature stress, cell membrane lipids undergo peroxidation reactions, rapidly accumulating malondialdehyde, destroying the membrane structure and increasing cell membrane permeability, causing plant metabolic disorders and preventing the life activities of other tissues and organs in the plant, ultimately leading to plant death. Existing research technologies are mostly focused on seed germination cold resistance research, while there are few studies on cold resistance of branches that have grown for a certain number of years.

[0006] In the prior art: fruit tree dormant branch cold resistance comparison method, Publication (Announcement) No.: CN114062436B, the cold resistance is tested by a resistance value curve, but the resistance value curve of the above test is not enough to fully test the cold resistance of the branches. Therefore, in view of the technical problems existing in the technical solutions involved in the above patents, a more comprehensive and effective testing method needs to be developed.

[0007] It should be noted that the above information disclosed in this background technology section is only used to understand the background technology of the present application concept, and therefore, the above description is not considered to constitute information of the prior art. Summary of the invention

[0008] The disclosed embodiments at least provide a method for testing cold resistance of wolfberry branches and a centrifuge thereof.

[0009] In a first aspect, the present disclosure provides a method for testing cold resistance of wolfberry branches, comprising the following steps:

[0010] Step S1: sampling, taking branches with a diameter of about 0.5-0.6 cm and uniform thickness, pruning and sealing;

[0011] Step S2: pre-treating the sample, cleaning the stains outside the sample and refrigerating it for later use;

[0012] Step S3: low temperature treatment of the sample, placing the sample in a low temperature program controlled container, setting the processing temperature gradient, and keeping the low temperature for a certain period of time, and then heating the sample;

[0013] Step S4: obtaining a supernatant, adding a quantitative amount of the extract to the sample and grinding it into a homogenate, placing the homogenate in a centrifuge for centrifugal separation, and obtaining a supernatant;

[0014] Step S5: Determine the MDA content in the supernatant.

[0015] In an optional embodiment, in the step S2, after treating the excess stains on the outer wall of the sample, the sample needs to be rinsed with deionized water multiple times to dry the moisture on the outer wall of the sample, and the end of the sample is sealed with wax and refrigerated.

[0016] In an optional embodiment, in S2, the treatment temperature gradient is set to -20°C, -25°C, -30°C, -35°C, starting from 0°C, the temperature decreases by 1°C every 10 minutes, and is maintained for 12 hours after reaching the set temperature;

[0017] Then raise the temperature by 1°C every 10 minutes to 0°C, take out and store in the refrigerator.

[0018] In an optional embodiment, the specific steps of determining the MDA content of the supernatant in S5 are as follows:

[0019] Preheat the spectrophotometer for more than 10 minutes, adjust the wavelength to 532nm or 600nm, and zero it with distilled water;

[0020] Pipette 0.6 ml of reagent into a 1.5 ml centrifuge tube, add 0.2 ml of sample, mix well, keep warm in a 95°C water bath for 30 min, and cool in an ice bath;

[0021] Centrifuge at 25°C, 10,000 rpm for 10 min;

[0022] The supernatant was aspirated into a 1 ml cuvette and the absorbance at wavelengths of 532 nm and 600 nm was measured, recorded as A532 and A600, ΔA = A532 - A600.

[0023] MDA 含量(nmol / mg) = [ΔA × V 反总 ÷ (ε × d) × 10 9 ] ÷ (W × V 样 ÷V 样总 )V 反总 :Total volume of reaction system, 8x10 -4 l

[0024] ε: molar extinction coefficient of malondialdehyde, 155x10 ³l / mol / cm;

[0025] d: cuvette light path, 1 cm;

[0026] V 样 : Added sample volume, 0.2ml;

[0027] V 样总 ; Volume of extract added, 1 mg;

[0028] W: sample mass, g.

[0029] In a second aspect, the present disclosure also provides a centrifuge, comprising:

[0030] Centrifuge body;

[0031] A fixed angle rotor, which is rotatably disposed in the centrifuge body and is conical in shape;

[0032] The fixed-angle rotor is provided with a plurality of placement holes at equal intervals, and the reagent tubes are suitable for being inserted into the placement holes;

[0033] A sensor is arranged in the centrifuge body near the fixed-angle rotor to monitor whether a reagent tube is placed in the placement hole;

[0034] An adjusting and limiting device, which is suitable for abutting against the side wall of the centrifuge body, and a plurality of the adjusting and limiting devices are arranged circumferentially around the fixed-angle rotor;

[0035] Wherein, when the fixed angle rotor rotates, the sensor is suitable for monitoring the placement hole to detect whether the reagent tube placed in the fixed angle rotor is balanced;

[0036] Adjust the limit device to draw suction or send air into the placement hole to give a warning or facilitate the removal of the reagent tube.

[0037] In an optional embodiment, a plurality of limit plates are arranged on the inner wall of the centrifuge body along the circumferential direction, and the limit plates face the fixed-angle rotor;

[0038] One limiting piece corresponds to one adjusting limiting device, and the limiting piece is suitable for limiting the adjusting limiting device to move upward.

[0039] In an optional embodiment, the adjustment and limiting device includes: a limiting member, which is in an arc shape and is lifted and arranged below the limiting piece;

[0040] The inner wall of the stopper close to the fixed-angle rotor is parallel to the fixed-angle rotor;

[0041] A connecting air pipe is arranged at the upper end of the limiting member and is connected to the air pump;

[0042] An exhaust groove is provided on the inner wall of the limiting member, and the exhaust groove is communicated with the connecting air pipe;

[0043] When the air pipe is connected to deliver compressed air to the exhaust groove, the limiting member is pushed by the compressed air to move in the direction of the limiting piece.

[0044] In an optional embodiment, an air hole is radially formed on the outer wall of the fixed-angle rotor, and the air hole faces the exhaust slot;

[0045] The fixed-angle rotor is provided with an annular groove near the bottom of the placement hole, and the annular groove is communicated with the air hole;

[0046] A communicating hole is formed on the bottom side wall of each placement hole, and the communicating hole is connected to the annular groove;

[0047] When the exhaust groove delivers compressed air into the annular groove, the compressed air is suitable for pushing the reagent tube in the placement hole to facilitate taking out the reagent tube.

[0048] In an optional embodiment, the number of the placement holes is 6, and the fixed-angle rotor needs to be balanced before it can rotate;

[0049] Among them, each placement hole is equipped with a reagent tube;

[0050] Or reagent tubes are placed in half of the placement holes, and several of the reagent tubes are placed at equal intervals.

[0051] In an optional implementation, the balancing formula for the fixed-angle rotor is:

[0052] S = ∑ 6 i=1 a i b i； a i=1， Indicates that there is a workpiece in the inspected station;

[0053] a i=0， Indicates that there is no workpiece in the inspected station;

[0054] b i=1， Indicates that there is no workpiece in the previous inspection station;

[0055] b i=-1， Indicates that there is a workpiece in the previous inspection station;

[0056] When s=3 or -6, the vertex rotor can rotate.

[0057] In an optional embodiment, the sensor is suitable for monitoring the volume of the slurry mixture in the reagent tube. If the volume is greater than 5% of the total volume of the reagent tube, the tracheal negative pressure is connected to suck the air in the placement hole to slow down the speed at which the reagent tube is taken out.

[0058] The beneficial effects of the present invention are that the cold resistance test method of black wolfberry branches and the centrifuge thereof of the present invention help to improve the accuracy and stability of the determination of the MDA content of the supernatant by pre-treating and low-temperature treating the sample branches, and at the same time, the method for determining the MDA content of the supernatant is simple and easy to operate. The centrifuge of the present invention, by adjusting the setting of the limit device, blows air to the outer wall of the fixed-angle rotor to press the fixed-angle rotor when the fixed-angle rotor rotates, thereby avoiding the situation that the fixed-angle rotor bottom nut is loosened and detached outward.

[0059] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention are realized and obtained by the structures particularly pointed out in the description, claims and drawings.

[0060] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, this article specifically cites preferred embodiments and provides detailed descriptions as follows in conjunction with the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0061] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0062] Figure 1 A schematic diagram of a method for testing cold resistance of wolfberry branches provided in an embodiment of the present disclosure;

[0063] Figure 2 A perspective view of a centrifuge provided in accordance with an embodiment of the present disclosure;

[0064] Figure 3 A three-dimensional view of the interior of a centrifuge body provided in an embodiment of the present disclosure;

[0065] Figure 4 A longitudinal sectional perspective view of a centrifuge provided in an embodiment of the present disclosure;

[0066] Figure 5 A cutaway perspective view of a fixed angle rotor provided in an embodiment of the present disclosure;

[0067] Figure 6 A schematic diagram of the states of a fixed-angle rotor and a stopper provided in an embodiment of the present disclosure;

[0068] Figure 7 A three-dimensional diagram of the adjustment and limiting device provided in an embodiment of the present disclosure.

[0069] In the figure:

[0070] 1. Centrifuge body; 10. Limiting piece; 2. Fixed-angle rotor; 20. Air hole; 21. Ring groove; 23. Connecting hole; 3. Placement hole; 4. Sensor; 5. Adjusting limiting device; 51. Limiting piece; 52. Connecting air pipe; 53. Exhaust groove. DETAILED DESCRIPTION

[0071] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0072] In this article, when it is mentioned that the first component is located on the second component, this may mean that the first component may be directly formed on the second component, or the third component may be interposed between the first component and the second component. In addition, in the drawings, in order to effectively describe the technical content, the thickness of the components may be exaggerated or reduced.

[0073] Herein, example embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. As used herein, expressions such as "at least one of..." modify the entire list of elements when following a list of elements, rather than modifying individual elements in the list. For example, the expression "at least one of a, b, and c" should be understood to include only a, only b, only c, both a and b, both a and c, both b and c, or all of a, b, and c.

[0074] The terms used herein are only used to describe specific exemplary configurations and are not intended to be limited. As used herein, the singular articles "one", "an" and "the" may also be intended to include plural forms, unless it is clearly indicated above that this is not the case. The terms "comprise", "include" and "have" are inclusive, and therefore specify the presence of features, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, elements, components and / or combinations thereof. The method steps, processes and operations described herein should not be interpreted as necessarily requiring them to be performed in the specific order discussed or shown, unless specifically identified as an execution order. Additional or alternative steps may be adopted.

[0075] As used herein, the phrases "in one embodiment," "according to one embodiment," "in some embodiments," and the like generally refer to the fact that the particular feature, structure, or characteristic following the phrase may be included in at least one embodiment of the present disclosure. Therefore, a particular feature, structure, or characteristic may be included in more than one embodiment of the present disclosure, so that these phrases do not necessarily refer to the same embodiment. As used herein, the terms "example," "exemplary," and the like are used to "serve as an example, instance, or illustration." Any implementation, aspect, or design described herein as "example" or "exemplary" is not necessarily to be construed as preferred or superior to other implementations, aspects, or designs. On the contrary, the use of the terms "example," "exemplary," and the like is intended to present concepts in a concrete manner.

[0076] The research found that the shortcomings of the existing technology are: in the related technology, when plants are subjected to low temperature stress, cell membrane lipids undergo peroxidation reactions, rapidly accumulate malondialdehyde, destroy the membrane structure and increase the permeability of the cell membrane, causing plant metabolic disorders and preventing the life activities of other tissues and organs in the plant, and ultimately leading to the death of the plant. The existing research technology is mostly focused on the cold resistance of seed germination, while there is little research on the cold resistance of branches that have grown for a certain number of years.

[0077] In the prior art: fruit tree dormant branch cold resistance comparison method, Publication (Announcement) No.: CN114062436B, the cold resistance is tested by a resistance value curve, but the resistance value curve of the above test is not enough to fully test the cold resistance of the branches. Therefore, it is necessary to develop a more comprehensive and effective testing method.

[0078] The defects existing in the above solutions are the results obtained by the inventor after practice and careful research. Therefore, the discovery process of the above problems and the solutions proposed by the present invention in this article for the above problems should be the contributions made by the inventor to the present invention during the disclosure process.

[0079] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0080] Some embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0081] like Figure 1 As shown, some embodiments provide a cold resistance test method for black wolfberry branches, including: S1: sampling, taking branches with a diameter of about 0.5-0.6cm and uniform thickness, pruning and sealing; when sampling, select branches with good growth, preferably, select 1-year-old lignified branches, mark and seal. S2: Pre-treat the sample, clean the stains outside the sample and refrigerate for standby use; S3: Low-temperature treatment of the sample, place the sample in a low-temperature program-controlled container, set the treatment temperature gradient, and keep the low temperature for a certain period of time, and then heat it up; S4: Obtain the supernatant, add the extract to the sample quantitatively, grind it into a homogenate, put the homogenate into a centrifuge for centrifugal separation, and take the supernatant; S5: Determine the MDA content of the supernatant. Pretreatment and low-temperature treatment of the sample branches can help improve the accuracy and stability of the determination of the MDA content in the supernatant. At the same time, the method for determining the MDA content in the supernatant is simple and easy to operate.

[0082] Reference Figure 1 , in the step S2, after the excess stains on the outer wall of the sample are processed, the sample needs to be rinsed with deionized water several times, the moisture on the outer wall of the sample is sucked dry, and the end of the sample is sealed with wax and refrigerated. During pretreatment, it is necessary to remove the excess yellow leaves and cut the branches into a length of 10 cm. Rinse with tap water, and use a soft brush to remove attached dirt, insect eggs, dust and other stains, then rinse with deionized water for no less than 3 times, and after drying the surface moisture, divide each numbered material into 5 equal parts, seal the end with wax, wrap it with wet gauze, and store it in a refrigerator at 4°C.

[0083] Reference Figure 1 In the step S2, the treatment temperature gradient is set to -20°C, -25°C, -30°C, -35°C, starting from 0°C, the temperature decreases by 1°C every 10 minutes, and is maintained for 12 hours after reaching the set temperature;

[0084] Then raise the temperature by 1°C every 10 minutes to 0°C, take out and refrigerate at 4°C. Before the experiment begins, avoid buds and cut the treated branches into thin slices of about 0.1 cm and mix them evenly.

[0085] Reference Figure 1 , the specific steps of S5 are as follows:

[0086] Weigh 0.1g of the treated branch slices, put them into a pre-cooled mortar, add a small amount of 5% TCA and quartz sand to grind into a homogenate, draw 0.6ml of reagent 6% TBA (thiobarbituric acid) solution into a 1.5ml centrifuge tube, add 0.2ml of the homogenate sample, mix well, keep warm in a 95℃ water bath for 30min, and cool in an ice bath; centrifuge the homogenate at 4℃, 8000r / min for 10min, and take the supernatant for testing. Preheat the spectrophotometer for more than 10min, adjust the wavelength to 532nm or 600nm, and adjust the zero with distilled water; centrifuge at 10000r / min for 10min; draw the supernatant into a 1ml colorimetric dish, and measure the absorbance at 532nm and 600nm wavelengths, recorded as A532 and A600, ΔA=A532-A600.

[0087] MDA 含量 = [ΔA × V 反总 ÷ (ε × d) × 109] ÷ (W × V 样 ÷V 样总 ) = 25.8 × ΔA ÷ W

[0088] V 反总 : Total volume of reaction system, 8x10-4l;

[0089] ε: molar extinction coefficient of malondialdehyde, 155x10 ³l / mol / cm;

[0090] d: cuvette light path, 1 cm;

[0091] V 样 : Added sample volume, 0.2ml;

[0092] V 样总 ; Volume of extract added, 1 mg;

[0093] W: sample mass, g;

[0094] Reference Figures 1 to 7Some embodiments provide a centrifuge, which is the cold resistance test method for black wolfberry branches in the above embodiment, that is, in step S4, the homogenate is placed in the centrifuge for centrifugal separation to obtain a supernatant; wherein the centrifuge comprises: a centrifuge body 1; the centrifuge body 1 is the centrifuge used in the cold resistance test method for black wolfberry branches, a fixed angle rotor 2, which is rotatably arranged in the centrifuge body 1 and is conical; a plurality of placement holes 3 are evenly spaced on the fixed angle rotor 2, and the reagent tube is suitable for inserting into the placement holes 3; a driving motor is arranged in the centrifuge body 1, and the fixed angle rotor 2 is bolted to the end of the rotating shaft of the driving motor, and the fixed angle rotor 2 is a replaceable part. Different fixed angle rotors 2 are replaced according to the different outer diameters of the reagent tubes required or the different numbers of reagent tubes that need to be centrifuged at the same time. The outer diameters of the fixed angle rotors 2 are the same, and the difference is the number and inner diameter of the placement holes 3. When replacing a different fixed-angle rotor 2, since the nut needs to be tightened manually, the nut of the fixed-angle rotor 2 may not be completely tightened. If the fixed-angle rotor 2 that is not completely tightened rotates at high speed, the fixed-angle rotor 2 may be separated from the centrifuge body 1. The sensor 4 is arranged in the centrifuge body 1 near the fixed-angle rotor 2 to monitor whether a reagent tube is placed in the placement hole 3; the adjustment limit device 5 is suitable for abutting against the side wall of the centrifuge body 1, and several of the adjustment limit devices 5 are arranged circumferentially around the fixed-angle rotor 2; wherein, when the fixed-angle rotor 2 rotates, the sensor 4 is suitable for monitoring the placement hole 3 to detect whether the reagent tube placed in the fixed-angle rotor 2 is balanced; the adjustment limit device 5 sucks or sends air into the placement hole 3 to warn or facilitate the removal of the reagent tube. When the fixed-angle rotor 2 rotates in the circumferential direction to centrifuge the liquid in the reagent tube, the adjusting limit device 5 delivers compressed air to the conical surface of the outer wall of the fixed-angle rotor 2. The compressed air is suitable for exerting a downward force on the fixed-angle rotor 2, thereby improving the stability of the fixed-angle rotor 2 in the centrifuge body 1. By adjusting the setting of the limit device 5, when the fixed-angle rotor 2 rotates, the outer wall of the fixed-angle rotor 2 is blown to press the fixed-angle rotor 2, thereby preventing the fixed-angle rotor 2 from detaching outward when the nut at the bottom of the fixed-angle rotor 2 is loosened.

[0095] Reference Figure 4 The inner wall of the centrifuge body 1 is provided with a plurality of limit plates 10 along the circumferential direction, and the limit plates 10 face the fixed-angle rotor 2; a gap is provided between the limit plates 10 and the conical surface of the outer wall of the fixed-angle rotor 2, so as to facilitate the replacement of the fixed-angle rotor 2. One limit plate 10 corresponds to one adjustment limit device 5, and the limit plate 10 is suitable for limiting the upward movement of the adjustment limit device 5. When the adjustment limit device 5 sprays compressed air to the conical surface of the top angle rotor, the limit member 51 moves upward along the inner wall and bottom wall of the centrifuge under the reverse force of the compressed air until it is abutted by the limit plate 10, and the limit plate 10 is suitable for preventing the limit member 51 from continuing to move upward.

[0096] Reference Figure 4 The regulating limit device 5 comprises: a limit member 51, which is in an arc shape and is arranged below the limit plate 10; the limit member 51 is arranged between the fixed angle rotor 2 and the inner wall of the centrifuge, and when the fixed angle rotor 2 does not rotate, the inner wall of the limit member 51 abuts against the conical surface of the fixed angle rotor 2. The limit member 51 is close to the inner wall of the fixed angle rotor 2 and is parallel to the fixed angle rotor 2; a connecting air pipe 52 is arranged at the upper end of the limit member 51 and is connected to the air pump; when the air pump delivers compressed air to the connecting air pipe 52, the compressed air is sprayed toward the fixed angle rotor 2 through the exhaust groove 53, and the limit member 51 moves toward the direction of the limit plate 10 under the reverse force of the compressed air. An exhaust groove 53 is provided on the inner wall of the limiting member 51, and the exhaust groove 53 is connected to the connecting air pipe 52; the air outlet of the exhaust groove 53 faces the fixed-angle rotor 2; wherein, when the connecting air pipe 52 delivers compressed air to the exhaust groove 53, the limiting member 51 is pushed by the compressed air to move in the direction of the limiting plate 10.

[0097] Reference Figure 5 , the outer wall of the fixed angle rotor 2 is provided with an air hole 20 in the radial direction, and the air hole 20 faces the exhaust groove 53; when the connecting air pipe 52 delivers compressed air into the exhaust groove 53, the compressed air is suitable to flow into the annular groove 21 along the air hole 20. The fixed angle rotor 2 is provided with an annular groove 21 near the bottom of the placement hole 3, and the annular groove 21 is connected with the air hole 20; each of the bottom side walls of the placement hole 3 is provided with a connecting hole 23, and the connecting hole 23 is connected with the annular groove 21; wherein, when the exhaust groove 53 delivers compressed air into the annular groove 21, the compressed air is suitable to push the reagent tube in the placement hole 3, so as to facilitate the removal of the reagent tube. When the fixed angle rotor 2 completes the centrifugal separation of the liquid in the reagent tube, the fixed angle rotor 2 stops rotating, at which time, the connecting air pipe 52 stops continuing to deliver compressed air into the exhaust groove 53, and the limiter 51 moves downward under the action of gravity until the inner wall of the limiter 51 abuts against the conical surface of the fixed angle rotor 2. When the connecting air pipe 52 continues to transport compressed air into the exhaust groove 53, the compressed air is suitable to flow into the connecting hole 23 through the air hole 20; and when the connecting air pipe 52 negatively sucks the air in the exhaust groove 53, the air in the annular groove 21 is negatively sucked by the air hole 20, so that the placement hole 3 is negatively sucked. At this time, when the reagent tube in the placement hole 3 is taken out, the resistance will increase, and the increase in resistance will alert the operator to be careful to hold the reagent tube.

[0098] Further, the sensor 4 is suitable for monitoring the volume of the slurry mixture in the reagent tube. If the volume is greater than 5% of the total volume of the reagent tube, that is, the volume of the slurry mixture contained in the solution in the reagent tube is relatively large, the reagent tube needs to be handled with care when it is taken out to avoid the slurry mixture being disturbed again and suspended upward in the solution. When the sensor 4 detects that the volume of the slurry mixture in the reagent tube to be taken out is relatively large, the air pump negatively draws the air pipe 52, and the air pipe 52 negatively draws the air in the placement hole 3 to slow down the speed at which the reagent tube is taken out.

[0099] Preferably, the number of the placement holes 3 is 6, and the fixed-angle rotor 2 needs to be balanced before it can rotate;

[0100] Among them, each placement hole 3 is placed with a reagent tube;

[0101] Or reagent tubes are placed in half of the placement holes 3, and several of the reagent tubes are placed at equal intervals.

[0102] The balancing formula of the fixed angle rotor 2 is:

[0103] S = ∑ 6 i=1 a i b i

[0104] ai=1, indicating that there is a workpiece in the inspected station;

[0105] ai=0, indicating that there is no workpiece in the inspected station;

[0106] bi=1, indicating that there is no workpiece in the previous inspection station;

[0107] bi=-1, indicating that there is a workpiece in the previous inspection station;

[0108] When s=3 or -6, the vertex rotor can rotate.

[0109] If a reagent tube is placed in each placement hole 3, that is, a1=1, and b1=-1, a2=1, and b2=-1, a3=1, and b3=-1, a4=1, and b4=-1, a5=1, and b5=-1, a6=1, and b6=-1, so s=-6;

[0110] If half of the placement holes 3 are filled with reagent tubes, and some of the reagent tubes are placed at equal intervals, that is, a1=1, and b1=1, a2=0, and b2=-1, a3=1, and b3=1, a4=0, and b4=-1, a5=1, and b5=1, a6=0, and b6=-1, so s=3;

[0111] Only when all six placement holes 3 are filled with reagent tubes, or three reagent tubes are placed in the placement holes 3 and the three reagent tubes are placed in the corresponding placement holes 3 at equal intervals, can the fixed-angle rotor 2 rotate normally.

[0112] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0113] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, terms such as "first", "second" and other numerical terms do not imply an order or sequence when used in this document unless explicitly indicated above. Therefore, without departing from the teachings of the example embodiments, the first element, component, region, layer or section discussed above may be referred to as a second element, component, region, layer or section.

[0114] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A centrifuge, which is applied to a method for testing cold resistance of wolfberry branches, characterized in that: The steps include: Step S1, sampling, taking branches with a diameter of about 0.5-0.6 cm and uniform thickness, pruning and sealing; Step S2, pre-treating the sample, cleaning the stains outside the sample and refrigerating it for later use; Step S3, low temperature treatment of the sample, placing the sample in a low temperature program controlled container, setting the treatment temperature gradient, and keeping the low temperature for a certain period of time, and then heating the sample; Step S4, obtaining a supernatant, adding a quantitative amount of the extract to the sample and grinding it into a homogenate, placing the homogenate in a centrifuge for centrifugal separation, and obtaining a supernatant; Step S5, measuring the MDA content of the supernatant; In step S4, the homogenate is placed in a centrifuge for centrifugal separation to obtain a supernatant; wherein the centrifuge comprises: Centrifuge body (1); A fixed-angle rotor (2) which is rotatably disposed in the centrifuge body (1) and is conical in shape; The fixed-angle rotor (2) is provided with a plurality of placement holes (3) at equal intervals, and the reagent tubes are suitable for being inserted into the placement holes (3); A sensor (4) is arranged in the centrifuge body (1) near the fixed-angle rotor (2) to monitor whether a reagent tube is placed in the placement hole (3); An adjusting and limiting device (5) adapted to abut against a side wall of the centrifuge body (1), wherein a plurality of the adjusting and limiting devices (5) are arranged circumferentially around the fixed-angle rotor (2); When the fixed-angle rotor (2) rotates, the sensor (4) is suitable for monitoring the placement hole (3) to detect whether the reagent tube placed in the fixed-angle rotor (2) is balanced; The limiting device (5) is adjusted to draw air or send gas into the placement hole (3) to give a warning or facilitate the removal of the reagent tube; The inner wall of the centrifuge body (1) is provided with a plurality of limit plates (10) along the circumferential direction, and the limit plates (10) face the fixed-angle rotor (2); A limiting piece (10) corresponds to an adjusting limiting device (5), and the limiting piece (10) is suitable for limiting the upward movement of the adjusting limiting device (5); The regulating limit device (5) comprises: a limit member (51) which is in an arc shape and is arranged to be lifted below the limit plate (10); The inner wall of the limiting member (51) close to the fixed-angle rotor (2) is parallel to the fixed-angle rotor (2); A connecting air pipe (52) is arranged at the upper end of the limiting member (51) and is connected to the air pump; An exhaust groove (53) is provided on the inner wall of the limiting member (51), and the exhaust groove (53) is in communication with the connecting air pipe (52); When the connecting air pipe (52) delivers compressed air to the exhaust groove (53), the limiting member (51) is pushed by the compressed air to move in the direction of the limiting plate (10).

2. The centrifuge according to claim 1, characterized in that An air hole (20) is radially formed on the outer wall of the fixed-angle rotor (2), and the air hole (20) faces the exhaust groove (53); The fixed-angle rotor (2) is provided with an annular groove (21) near the bottom of the placement hole (3), and the annular groove (21) is communicated with the air hole (20); A communicating hole (23) is formed on the bottom side wall of each placement hole (3), and the communicating hole (23) is connected to the annular groove (21); When the exhaust groove (53) delivers compressed air into the annular groove (21), the compressed air is suitable for pushing the reagent tube in the placement hole (3) to facilitate the removal of the reagent tube.

3. The centrifuge according to claim 1, characterized in that There are six placement holes (3), and the fixed-angle rotor (2) needs to be balanced before it can rotate; Wherein, each placement hole (3) is provided with a reagent tube; Or reagent tubes are placed in half of the placement holes (3), and a plurality of the reagent tubes are placed at equal intervals.

4. The centrifuge according to claim 3, characterized in that The balancing formula of the fixed angle rotor (2) is: S = ∑ 6 i=1 a i b i ; a i =1, indicating that there is a workpiece in the inspected station; a i =0, indicating that there is no workpiece in the inspected station; b i =1, indicating that there is no workpiece in the previous inspection station; b i =-1, indicating that there is a workpiece in the previous inspection station; When s=3 or -6, the vertex rotor can rotate.

5. The centrifuge according to claim 2, characterized in that The sensor (4) is suitable for monitoring the volume of the slurry mixture in the reagent tube. If the volume is greater than 5% of the total volume of the reagent tube, the air pipe (52) is connected to negatively pressure the air in the placement hole (3) to slow down the speed at which the reagent tube is taken out.

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