Immunity enhancing feed containing natural plant extract for pigs and preparation method of immunity enhancing feed

Abstract

The invention relates to a pig immunity-enhancing feed containing natural plant extracts, and belongs to the technical field of feeds. On the basis of basic components, a plantain herb and selfheal composite extract, a purslane and sweet potato vine and leaf composite extract and a folium cortex eucommiae extract are also added into the feed, so that the immunity-enhancing feed is prepared. The plantain herb and selfheal composite extract is obtained by sequentially fermenting a plantain herb whole plant and a selfheal whole plant through lactobacillus cellobiosus and pediococcus acidilactici and then sequentially carrying out step-by-step enzymolysis through trypsin and nattokinase; the herba portulacae and sweet potato vine compound extract is obtained by sequentially fermenting a whole plant of herba portulacae and leaves of sweet potato vines with lactobacillus cellobiosus and bacteroides ruminalis and then sequentially carrying out step-by-step enzymolysis with snail protease and trypsin; the folium cortex eucommiae extract is obtained by sequentially carrying out step-by-step enzymolysis on folium cortex eucommiae through hemicellulase, bromelain and nattokinase. The extract prepared by the invention contains immunological enhancement effective components, and can enhance the immunity of pigs, effectively reduce diarrhea and reduce the risk of illness when being matched with basal feed for use.

Description

Technical Field

[0001] The invention belongs to the technical field of feed, and in particular relates to an immune enhancing feed for pigs containing natural plant extracts and a preparation method thereof. Background Art

[0002] As one of the world's major meat products, pork quality and safety are directly related to people's health. The strength of pig immunity is a key factor affecting pork quality and breeding efficiency. In the past, traditional pig feeds mainly focused on meeting the growth needs of pigs, providing sufficient energy and nutrients to promote pig weight gain. However, this single goal often ignores the development and maintenance of the pig's immune system.

[0003] In recent years, with the changes in the breeding environment and the frequent occurrence of various epidemics, the limitations of traditional pig feed have become increasingly prominent. On the one hand, the high-density breeding model makes pigs more vulnerable to pathogens. In a small space, pigs frequently come into contact with each other. Once an epidemic occurs, it is easy to spread quickly, causing huge economic losses to farmers. On the other hand, unreasonable feed formulas may lead to nutritional imbalances in pigs, thereby affecting the normal function of their immune systems. For example, the lack of certain key vitamins, minerals or amino acids may reduce the immunity of pigs and increase the risk of disease.

[0004] The pig's immune system is a complex network composed of multiple cells and molecules that work together to resist the invasion of pathogens. The nutrients in feed can directly or indirectly affect the pig's immune system. For example, certain vitamins and minerals such as vitamin C, vitamin E, and zinc have antioxidant effects and can protect immune cells from damage by free radicals; proteins and amino acids are important components of immune molecules such as immunoglobulins; probiotics and prebiotics can regulate intestinal flora and enhance intestinal immune function.

[0005] In today's livestock farming industry, the development of pig feed plays a vital role in pig farming. As people pay more and more attention to food safety and animal health, the research and development and application of pig feed that enhances immunity has gradually become the focus. For example, genetic engineering technology and biofermentation technology are used to prepare feed ingredients rich in specific nutrients to enhance the immunity of pigs.

[0006] Faced with an increasingly deteriorating environment and evolving viruses, the effectiveness of existing pig feed in enhancing immunity is mixed. Generally, the effect of improving immunity is poor and the morbidity rate remains high. Therefore, it is necessary to continuously develop and improve technology, improve feed efficacy, reduce livestock breeding risks, improve breeding efficiency, ensure the quality and safety of pork, and contribute to promoting the sustainable development of animal husbandry. Summary of the invention

[0007] The existing pig feeds have different effects in enhancing immunity, and generally have poor effects in improving immunity. The present invention provides an immune-enhancing feed for pigs containing natural plant extracts and a preparation method thereof. On the basis of the basic feed, a composite extract of plantain and selfheal, a composite extract of purslane and sweet potato vine leaves, and an extract of eucommia leaves prepared by a special method are added. The various extracts are used in combination with the basic feed to well enhance the immunity of pigs, effectively reduce diarrhea, and reduce the risk of disease. The specific technical scheme is as follows:

[0008] The invention discloses an immune-enhancing feed for pigs containing natural plant extracts, the feed comprising the following raw materials in parts by mass: 0.5 to 1.5 parts of a composite extract of plantain and selfheal, 0.5 to 1.5 parts of a composite extract of purslane and sweet potato vine leaves, 0.1 to 0.3 parts of an extract of eucommia leaves, 60 to 65 parts of corn flour, 15 to 25 parts of soybean meal powder, 3 to 17 parts of wheat bran powder, 0 to 5 parts of fish meal, 0 to 1 part of calcium hydrogen phosphate, and 0 to 5 parts of whey powder.

[0009] The plantain and selfheal composite extract is a product obtained by fermenting the whole plant of plantain and the whole plant of selfheal in sequence with Lactobacillus cellobiosaccharide and Pediococcus acidilactici, and then enzymolysis by trypsin and nattokinase in sequence; the purslane and sweet potato vine composite extract is a product obtained by fermenting the whole plant of purslane and sweet potato vine leaves with Lactobacillus cellobiosaccharide and Bacteroides rumenoides in sequence, and then enzymolysis by snail protease and trypsin in sequence; the eucommia leaf extract is a product obtained by enzymolysis of eucommia leaves with hemicellulase, bromelain and nattokinase in sequence.

[0010] In the above feed, the feed also includes 0 to 4 parts of premix, and the premix includes vitamins, minerals, antioxidants, mildew inhibitors, and flavoring agents.

[0011] In the above feed, the preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0012] According to the mass ratio, plantain: selfheal = (5-10): (3-5), the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 10-15 times the mass of water of the base material is added to the base material, 3%-5% of glucose by mass of the base material, 0.3%-0.8% of peptone by mass of the base material, and 0.03%-0.05% of dipotassium hydrogen phosphate by mass of the base material are added, the pH is adjusted to 6.0-6.4, 1%-2.5% of Lactobacillus cellobiose by mass of the base material and 0.5%-1% of Pediococcus acidilactici by mass of the base material are added, and spray fermentation is carried out at 30°C-35°C for 25h-30h, the pH is adjusted to 8-8.5, 0.5%-1% of trypsin by mass of the base material is added, and enzymolysis is carried out at 35°C-37°C for 40min n~60min, then add 0.5%~1% of nattokinase by weight of the base material, carry out enzymolysis at 45℃~55℃ for 60min~90min, adjust the pH to 6.5~7.5, sieve, take the sieve, centrifuge, take the solid, dry, and obtain the composite extract of plantain and selfheal.

[0013] In the above feed, the preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0014] According to the mass ratio, purslane: sweet potato vine leaves = (1-3): (5-15), the whole purslane plant and the sweet potato vine leaves are mixed and crushed to obtain a base material, 10 to 15 times the mass of water of the base material is added to the base material, 3% to 5% of glucose by mass of the base material, 0.3% to 0.8% of peptone by mass of the base material, and 0.03% to 0.05% of dipotassium hydrogen phosphate by mass of the base material are added, the pH value is adjusted to 6.0 to 6.4, 1% to 2.5% of Lactobacillus cellobiose by mass of the base material is added, and the mixture is sprayed and fermented at 30°C to 35°C for 20h to 24h, the pH value is adjusted to 6.0 to 7.0, and 0.5% to 1% of the mass of the base material Bacteroides rumensis is fermented anaerobically at 39°C to 41°C for 15h to 20h, the pH is adjusted to 5.5 to 6.5, 0.5% to 1.5% of the mass of the base material snail protease is added, enzymolysis is carried out at 35°C to 40°C for 50min to 80min, then the pH is adjusted to 8 to 8.5, 0.5% to 1% of the mass of the base material trypsin is added, enzymolysis is carried out at 35°C to 37°C for 40min to 60min, the pH is adjusted to 6.5 to 7.5, sieving, taking the sieve, centrifuging, taking the solid, and drying to obtain a composite extract of purslane and sweet potato vine.

[0015] In the above feed, the preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0016] Take eucommia leaves and grind them, add water 8 to 10 times the mass of the eucommia leaves, adjust the pH to 4.5 to 5.5, add hemicellulase 0.5 to 2% by mass of the eucommia leaves, perform enzymolysis at 50 to 55° C. for 3 hours to 4 hours, adjust the pH to 6.5 to 8.0, add bromelain 0.5 to 2% by mass of the eucommia leaves, perform enzymolysis at 50 to 55° C. for 50 to 80 minutes, inactivate the enzyme at 80 to 85° C. for 10 to 15 minutes, cool to room temperature, adjust the pH to 8 to 8.5, add nattokinase 0.5 to 1% by mass of the base material, perform enzymolysis at 45 to 55° C. for 60 to 90 minutes, adjust the pH to 6.5 to 7.5, sieve, take the sieve, centrifuge, take the solid, and dry to obtain the eucommia leaf extract.

[0017] In the preparation method of the above-mentioned plantain and selfheal composite extract, the bacterial activity of the cellobiose Lactobacillus is 1 billion CFU / g to 5 billion CFU / g; the bacterial activity of the acidilactic Pediococcus is 1 billion CFU / g to 5 billion CFU / g; the enzyme activity of the trypsin is 100,000 U / g to 500,000 U / g; and the enzyme activity of the nattokinase is 10,000 U / g to 100,000 U / g.

[0018] In the preparation method of the above-mentioned purslane and sweet potato vine composite extract, the bacterial activity of the cellobiose Lactobacillus is 1 billion CFU / g to 3 billion CFU / g; the bacterial activity of the rumen Bacteroides is 1 billion CFU / g to 5 billion CFU / g; the enzymatic activity of the snail protease is 5,000 U / g to 10,000 U / g; and the enzymatic activity of the trypsin is 100,000 U / g to 300,000 U / g.

[0019] In the preparation method of the above-mentioned eucommia leaf extract, the enzymatic activity of the hemicellulase is 50,000 U / g to 100,000 U / g; the enzymatic activity of the bromelain is 100,000 U / g to 500,000 U / g; and the enzymatic activity of the nattokinase is 20,000 U / g to 100,000 U / g.

[0020] In the above technical solution, the mesh size of the sieving sieve is 100-200 mesh; the rotation speed of the centrifugal separation is 3000r / min-6000r / min, the time of the centrifugal separation is 20min-40min; and the drying temperature is 65°C-75°C.

[0021] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0022] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0023] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0024] S3: Mix the mixture A and the mixture B to obtain feed.

[0025] Compared with the prior art, the immune-enhancing feed for pigs containing natural plant extracts and the preparation method thereof of the present invention have the following beneficial effects:

[0026] 1. The feed of the present invention, on the basis of basic feed, also contains composite extracts of plantain and selfheal, composite extracts of purslane and sweet potato vine leaves, and extracts of eucommia leaves. The various extracts are used in combination with the basic feed to well enhance the immunity of pigs.

[0027] 2. The composite extract of plantain and selfheal is: the whole plant of plantain and selfheal is fermented by cellobiose lactobacillus and lactic acid pediococcus in turn, and then enzymatically hydrolyzed by trypsin and nattokinase in turn, to obtain the product; the product contains a variety of antioxidant ingredients, such as flavonoids, phenols and other substances, as well as fermentation products and specific small molecule peptides after enzymatic hydrolysis in turn, which can neutralize excessive free radicals in pigs and reduce the damage of oxidative stress to cells. Free radicals are produced during normal metabolism, but too many free radicals can damage cell structure and function and affect the normal functioning of the immune system. By improving antioxidant capacity, protecting immune cells from oxidative damage and maintaining their normal physiological functions, the immunity of pigs is enhanced. The product can also stimulate the proliferation of immune cells in pigs, such as lymphocytes and macrophages. Lymphocytes are an important part of the immune system and play a key role in cellular immunity and humoral immunity. Increasing the number of immune cells can improve the immune defense ability of pigs. The product can also regulate the intestinal flora of pigs, promote the growth of beneficial bacteria, and inhibit the reproduction of harmful bacteria. Healthy intestinal flora is essential for maintaining the integrity of the intestinal mucosal barrier and is also involved in immune regulation. Some ingredients in the product have anti-inflammatory properties, which can inhibit the over-activation of inflammatory responses and help maintain the stability and normal function of the immune system.

[0028] 3. The composite extract of purslane and sweet potato vine is: the whole plant of purslane and sweet potato vine leaves are fermented by cellobiose lactobacillus and rumen bacteroides in turn, and then hydrolyzed by snail protease and trypsin in turn. The obtained product contains fermentation products and specific small molecule peptides after hydrolysis. Purslane and sweet potato vines themselves contain a variety of nutrients (protein, vitamins, minerals, etc.). After fermentation and hydrolysis, these nutrients are easier to be absorbed and utilized by pigs. Adequate nutrition helps the growth and development of pigs, making the pigs healthier and stronger, and providing a material basis for the normal operation of the immune system. Some components in the extract can act as prebiotics, selectively promoting the growth and reproduction of beneficial bacteria in the intestine (such as bifidobacteria, lactobacilli, etc.). Beneficial bacteria can produce beneficial metabolites such as short-chain fatty acids, lower the intestinal pH, inhibit the growth of harmful bacteria, reduce the colonization of harmful bacteria (such as Escherichia coli, Salmonella, etc.) in the intestine, prevent harmful bacteria from producing toxins that damage the intestinal mucosa, and maintain a good balance of intestinal flora, which helps protect the intestinal mucosal barrier, reduce intestinal inflammatory reactions, and improve intestinal immunity. Purslane and sweet potato vine extracts contain antioxidants such as flavonoids and phenolic acids. These antioxidant ingredients can remove free radicals in pigs, reduce the damage of oxidative stress to cells, and protect immune cells such as lymphocytes and macrophages from free radical attacks by enhancing antioxidant capacity, thereby maintaining their normal immune function.

[0029] The extract contains some small molecule peptides and bioactive ingredients that can stimulate immune cells in pigs, such as T lymphocytes, B lymphocytes and macrophages. Activated immune cells can more effectively identify and eliminate pathogens, enhancing the immune defense of pigs. Immune cells are induced to secrete cytokines (such as interferon, interleukin, etc.), which play an important role in immune regulation, coordinate the interaction between immune cells, and enhance immune response.

[0030] 4. Eucommia leaf extract is: the product obtained by enzymatic hydrolysis of Eucommia leaves with hemicellulase, bromelain and nattokinase in sequence; the enzymatic extract of Eucommia leaves contains a variety of antioxidant ingredients, such as chlorogenic acid, flavonoids, etc., and also contains small molecule peptides with specific graded enzymatic hydrolysis. These ingredients can remove free radicals in pigs and reduce the damage of oxidative stress to cells. Through antioxidant action, immune cells are protected from oxidative damage and their normal physiological functions are maintained, thereby improving the immunity of pigs. The extract promotes the proliferation of T lymphocytes and B lymphocytes. T lymphocytes play a key role in cellular immunity and can recognize and attack cells infected by pathogens; B lymphocytes are responsible for producing antibodies and participate in humoral immunity. The proliferation of lymphocytes can increase the number of immune cells and improve the immune defense ability of pigs. The enzymatic extract of Eucommia leaves can regulate the secretion of cytokines in pigs. Cytokines are a class of small molecule proteins secreted by immune cells and play an important role in immune regulation. For example, it promotes the secretion of cytokines such as interferon and interleukin, which can enhance the activity of immune cells, coordinate immune responses, and improve the immunity of pigs. DETAILED DESCRIPTION

[0031] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0032] Example 1

[0033] A pig immunity enhancing feed containing natural plant extracts, the feed comprising the following raw materials in parts by mass: 1 part of plantain and selfheal compound extract, 1 part of purslane and sweet potato vine leaf compound extract, 0.2 part of eucommia leaf extract, 62 parts of corn flour, 20 parts of soybean meal powder, 10 parts of wheat bran powder, 2.5 parts of fish meal, 0.5 parts of calcium hydrogen phosphate, and 2 parts of whey powder.

[0034] The preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0035] According to the mass ratio of plantain to selfheal = 7.5:4, the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 12 times the mass of water of the base material is added to the base material, 4% of the mass of glucose, 0.5% of the mass of peptone, and 0.04% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.2, 1.5% of the mass of Lactobacillus cellobiose with a bacterial activity of 3 billion CFU / g and 0.8% of the mass of lactic acid tablets with a bacterial activity of 3 billion CFU / g are added to the base material. bacteria, spray fermentation at 32°C for 28h, adjust pH8.2, add 0.8% of the base material weight of trypsin with an enzyme activity of 300,000 U / g, enzymolysis at 36°C for 50min, then add 0.6% of the base material weight of nattokinase with an enzyme activity of 50,000 U / g, enzymolysis at 50°C for 75min, adjust pH7.0, sieve through 150 mesh, take the sieve, centrifuge at 4000r / min for 30min, take the solid, and dry at 70°C to obtain a composite extract of plantain and selfheal.

[0036] The preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0037] According to the mass ratio, purslane: sweet potato vine leaves = 1.5:12, the whole plant of purslane and the sweet potato vine leaves are mixed and crushed to obtain a base material, 12 times the mass of water of the base material is added to the base material, 4% of the mass of glucose, 0.5% of the mass of peptone, and 0.04% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.2, 1.5% of the mass of Lactobacillus cellobiose with a bacterial activity of 2 billion CFU / g is added to the base material, and the fermentation is sprayed at 32°C for 22h, the pH is adjusted to 6.5, and 0.8% of the mass of the base material with a bacterial activity of 3 billion CFU / g is added. FU / g of rumen Bacteroides, anaerobically fermented at 40°C for 18h, adjusted to pH6.0, added snail protease with an enzyme activity of 10,000 U / g at 1% of the base mass, enzymatically hydrolyzed at 37°C for 65min, then adjusted to pH8.2, added trypsin with an enzyme activity of 200,000 U / g at 0.8% of the base mass, enzymatically hydrolyzed at 36°C for 50min, adjusted to pH7.0, sieved through 150 mesh, took the sieve, centrifuged at 4000r / min for 30min, took the solid, and dried at 70°C to obtain a composite extract of purslane and sweet potato vine.

[0038] The preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0039] The eucommia leaves are crushed, water of 9 times the mass of the eucommia leaves is added, the pH is adjusted to 5.0, hemicellulase with an enzyme activity of 100,000 U / g in an amount of 1.2% by mass of the eucommia leaves is added, enzymolysis is performed at 52° C. for 3.5 hours, the pH is adjusted to 7.2, bromelain with an enzyme activity of 300,000 U / g in an amount of 1.2% by mass of the eucommia leaves is added, enzymolysis is performed at 52° C. for 65 minutes, the enzyme is inactivated at 82° C. for 15 minutes, the pH is adjusted to 8.2 after cooling to room temperature, nattokinase with an enzyme activity of 100,000 U / g in an amount of 0.8% by mass of the base material is added, enzymolysis is performed at 50° C. for 80 minutes, the pH is adjusted to 7.0, 150 meshes are sieved, the sieve is taken, centrifuged at 4000 r / min for 30 minutes, the solid is taken, and dried at 70° C. to obtain an eucommia leaf extract.

[0040] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0041] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0042] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0043] S3: Mix the mixture A and the mixture B to obtain feed.

[0044] Example 2

[0045] A pig immunity enhancing feed containing natural plant extracts, the feed comprising the following raw materials in parts by mass: 0.5 parts of plantain and selfheal compound extract, 0.5 parts of purslane and sweet potato vine leaf compound extract, 0.1 parts of eucommia leaf extract, 60 parts of corn flour, 15 parts of soybean meal powder, 3 parts of wheat bran powder, 0.2 parts of calcium hydrogen phosphate, and 0.5 parts of whey powder.

[0046] The preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0047] According to the mass ratio of plantain to selfheal = 10:5, the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 15 times the mass of water of the base material is added to the base material, 5% of the mass of glucose, 0.8% of the mass of peptone, and 0.05% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.4, 2.5% of the mass of Lactobacillus cellobiose with a bacterial activity of 5 billion CFU / g and 1% of the mass of lactic acid tablets with a bacterial activity of 5 billion CFU / g are added to the base material. bacteria, spray fermentation at 35°C for 30h, adjust pH8.5, add 1% of the base material mass of trypsin with an enzyme activity of 500,000 U / g, enzymolysis at 37°C for 60min, then add 1% of the base material mass of nattokinase with an enzyme activity of 100,000 U / g, enzymolysis at 55°C for 90min, adjust pH7.5, sieve through 200 mesh, take the sieve, centrifuge at 6000r / min for 40min, take the solid, and dry it at 75°C to obtain a composite extract of plantain and selfheal.

[0048] The preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0049] According to the mass ratio, purslane: sweet potato vine leaves = 3:15, the whole plant of purslane and the sweet potato vine leaves are mixed and crushed to obtain a base material, 15 times the mass of water of the base material is added to the base material, 5% of the mass of glucose, 0.8% of the mass of peptone, and 0.05% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.4, 2.5% of the mass of Lactobacillus cellobiose with a bacterial activity of 3 billion CFU / g is added to the base material, and the fermentation is sprayed at 35°C for 24 hours, the pH is adjusted to 7.0, and 1% of the mass of the base material with a bacterial activity of 5 billion CFU / g is added. / g of rumen Bacteroides, anaerobically fermented at 41°C for 20h, adjusted to pH6.5, added snail protease with an enzyme activity of 10,000 U / g at 1.5% of the base material mass, enzymolysis at 40°C for 80min, then adjusted to pH8.5, added trypsin with an enzyme activity of 300,000 U / g at 1% of the base material mass, enzymolysis at 37°C for 60min, adjusted to pH7.5, sieved with 200 mesh, took the sieve, centrifuged at 6000r / min for 40min, took the solid, and dried at 75°C to obtain a composite extract of purslane and sweet potato vine.

[0050] The preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0051] The eucommia leaves are crushed, water with an activity of 10 times the mass of the eucommia leaves is added, the pH is adjusted to 5.5, 2% of the mass of the eucommia leaves with an enzyme activity of 100,000 U / g hemicellulase is added, enzymolysis is performed at 55° C. for 4 hours, the pH is adjusted to 8.0, 2% of the mass of the eucommia leaves with an enzyme activity of 500,000 U / g bromelain is added, enzymolysis is performed at 55° C. for 80 minutes, the enzyme is inactivated at 85° C. for 15 minutes, the pH is adjusted to 8.5 after cooling to room temperature, 1% of the mass of the base material with an enzyme activity of 100,000 U / g nattokinase is added, enzymolysis is performed at 55° C. for 90 minutes, the pH is adjusted to 7.5, 200 meshes are sieved, the sieve is taken, centrifuged at 6000 r / min for 40 minutes, the solid is taken, and it is dried at 75° C. to obtain the eucommia leaf extract.

[0052] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0053] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0054] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0055] S3: Mix the mixture A and the mixture B to obtain feed.

[0056] Example 3

[0057] An immune-enhancing feed for pigs containing natural plant extracts, the feed includes the following raw materials by weight: 1.5 parts of plantain and selfheal compound extract, 1.5 parts of purslane and sweet potato vine leaf compound extract, 0.3 parts of eucommia leaf extract, 65 parts of corn flour, 25 parts of soybean meal, 17 parts of wheat bran powder, 5 parts of fish meal. The feed also includes 2 parts of premixes, which include 0.4 parts of vitamins, 0.5 parts of minerals (stone powder), 0.05 parts of antioxidants (tea polyphenols), 0.05 parts of mildew inhibitors (potassium sorbate), and 1 part of flavoring (salt).

[0058] The preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0059] According to the mass ratio of plantain to selfheal = 5:3, the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 10 times the mass of water of the base material is added to the base material, 3% of the mass of glucose, 0.3% of the mass of peptone, and 0.03% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.0, 1% of the mass of Lactobacillus cellobiose with a bacterial activity of 1 billion CFU / g and 0.5% of the mass of Pediococcus acidilactici with a bacterial activity of 1 billion CFU / g are added to the base material , spray fermentation at 30°C for 25h, adjust pH8, add 0.5% of the base material mass of trypsin with an enzyme activity of 100,000 U / g, enzymolysis at 35°C for 40min, then add 0.5% of the base material mass of nattokinase with an enzyme activity of 10,000 U / g, enzymolysis at 45°C for 60min, adjust pH6.5, sieve through 100 mesh, take the sieve, centrifuge at 3000r / min for 20min, take the solid, and dry at 65°C to obtain a composite extract of plantain and selfheal.

[0060] The preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0061] According to the mass ratio of purslane to sweet potato vine leaves = 1:5, the whole plant of purslane and the sweet potato vine leaves are mixed and crushed to obtain a base material, 10 times the mass of water of the base material is added to the base material, 3% of glucose by mass of the base material, 0.3% of peptone by mass of the base material, and 0.03% of dipotassium hydrogen phosphate by mass of the base material are added, the pH value is adjusted to 6.0, 1% of Lactobacillus cellobiose with a bacterial activity of 1 billion CFU / g by mass of the base material is added, and the mixture is sprayed and fermented at 30°C for 20 hours, the pH value is adjusted to 6.0, and the base material is added. 0.5% by weight of rumen Bacteroides with an activity of 1 billion CFU / g was anaerobically fermented at 39°C for 15 hours, the pH was adjusted to 5.5, 0.5% by weight of the base material of snail protease with an enzyme activity of 5,000 U / g was added, and enzymolysis was carried out at 35°C for 50 minutes, then the pH was adjusted to 8, 0.5% by weight of the base material of trypsin with an enzyme activity of 100,000 U / g was added, and enzymolysis was carried out at 35°C for 40 minutes, the pH was adjusted to 6.5, 100 mesh sieve was passed, the sieve was taken, and the solid was centrifuged at 3000 r / min for 20 minutes. The solid was taken and dried at 65°C to obtain a composite extract of purslane and sweet potato vine.

[0062] The preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0063] The eucommia leaves are crushed, water of 8 times the mass of the eucommia leaves is added, the pH is adjusted to 4.5, hemicellulase with an enzyme activity of 50,000 U / g in an amount of 0.5% by mass of the eucommia leaves is added, enzymolysis is performed at 50°C for 3 hours, the pH is adjusted to 6.5, bromelain with an enzyme activity of 100,000 U / g in an amount of 0.5% by mass of the eucommia leaves is added, enzymolysis is performed at 50°C for 50 minutes, the enzyme is inactivated at 80°C for 10 minutes, the pH is adjusted to 8 after cooling to room temperature, nattokinase with an enzyme activity of 20,000 U / g in an amount of 0.5% by mass of the base material is added, enzymolysis is performed at 45°C for 60 minutes, the pH is adjusted to 6.5, 100 meshes are sieved, the sieve is taken, centrifuged at 3000 r / min for 20 minutes, the solid is taken, and dried at 65°C to obtain an eucommia leaf extract.

[0064] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0065] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0066] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0067] S3: Mix the mixture A and the mixture B to obtain feed.

[0068] Example 4

[0069] A pig immunity enhancing feed containing natural plant extracts, the feed comprising the following raw materials in parts by mass: 0.5 parts of plantain and selfheal compound extract, 1.5 parts of purslane and sweet potato vine leaf compound extract, 0.1 parts of eucommia leaf extract, 65 parts of corn flour, 15 parts of soybean meal powder, 17 parts of wheat bran powder, 1 part of calcium hydrogen phosphate, and 5 parts of whey powder.

[0070] The preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0071] According to the mass ratio of plantain to selfheal = 6.5:3.5, the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 10 times the mass of water of the base material is added to the base material, 5% of the mass of glucose, 0.3% of the mass of peptone, and 0.05% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.0, and 2.5% of the mass of Lactobacillus cellobiose with a bacterial activity of 1 billion CFU / g and 1% of the mass of Pediococcus acidilactici with a bacterial activity of 1 billion CFU / g are added to the base material. , spray fermentation at 35°C for 25h, adjust pH8.5, add 0.5% of the base material mass of trypsin with an enzyme activity of 500,000 U / g, enzymolysis at 35°C for 60min, then add 0.5% of the base material mass of nattokinase with an enzyme activity of 100,000 U / g, enzymolysis at 45°C for 90min, adjust pH6.5, sieve through 200 mesh, take the sieve, centrifuge at 3000r / min for 40min, take the solid, and dry at 65°C to obtain a composite extract of plantain and selfheal.

[0072] The preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0073] According to the mass ratio, purslane: sweet potato vine leaves = 3:5, the whole plant of purslane and the sweet potato vine leaves are mixed and crushed to obtain a base material, 15 times the mass of water of the base material is added to the base material, 3% of the mass of glucose, 0.8% of the mass of peptone, and 0.03% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.4, 1% of the mass of Lactobacillus cellobiose with a bacterial activity of 3 billion CFU / g is added to the base material, and the fermentation is sprayed at 30°C for 24 hours, the pH is adjusted to 6.0, and 1% of the mass of the base material with a bacterial activity of 1 billion CFU / g is added. g of rumen Bacteroides, anaerobically fermented at 41°C for 15h, adjusted to pH6.5, added snail protease with an enzyme activity of 10,000 U / g at 0.5% of the base material mass, enzymatically hydrolyzed at 35°C for 80min, then adjusted to pH8, added trypsin with an enzyme activity of 100,000 U / g at 1% of the base material mass, enzymatically hydrolyzed at 37°C for 40min, adjusted to pH7.5, sieved through 100 mesh, took the sieve, centrifuged at 6000r / min for 20min, took the solid, and dried at 75°C to obtain a composite extract of purslane and sweet potato vine.

[0074] The preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0075] The eucommia leaves are crushed, water of 8 times the mass of the eucommia leaves is added, the pH is adjusted to 5.5, hemicellulase with an enzyme activity of 100,000 U / g in an amount of 0.5% by mass of the eucommia leaves is added, enzymolysis is performed at 50° C. for 4 hours, the pH is adjusted to 6.5, bromelain with an enzyme activity of 100,000 U / g in an amount of 2% by mass of the eucommia leaves is added, enzymolysis is performed at 55° C. for 50 minutes, the enzyme is inactivated at 85° C. for 15 minutes, the pH is adjusted to 8.5 after cooling to room temperature, nattokinase with an enzyme activity of 100,000 U / g in an amount of 0.5% by mass of the base material is added, enzymolysis is performed at 45° C. for 90 minutes, the pH is adjusted to 6.5, sieved with 200 meshes, the sieve is taken, centrifuged at 3000 r / min for 40 minutes, the solid is taken, and dried at 65° C. to obtain an eucommia leaf extract.

[0076] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0077] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0078] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0079] S3: Mix the mixture A and the mixture B to obtain feed.

[0080] Example 5

[0081] An immune-enhancing feed for pigs containing natural plant extracts, the feed includes the following raw materials by weight: 1.5 parts of plantain and selfheal compound extract, 0.5 parts of purslane and sweet potato vine leaf compound extract, 0.3 parts of eucommia leaf extract, 60 parts of corn flour, 25 parts of soybean meal powder, and 3 parts of wheat bran powder. The feed also includes 4 parts of premixes, which include 0.8 parts of vitamins, 1 part of minerals (stone powder), 0.15 parts of antioxidants (tea polyphenols), 0.05 parts of antifungal agents (potassium sorbate), and 2 parts of flavoring agents (salt).

[0082] The preparation method of the composite extract of plantain and selfheal comprises the following steps:

[0083] According to the mass ratio of plantain to selfheal = 10:3, the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 15 times the mass of water of the base material is added to the base material, 3% of the mass of glucose, 0.8% of the mass of peptone, and 0.03% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.4, 1% of the mass of the base material is added with Lactobacillus cellobiose with a bacterial activity of 1 billion CFU / g, and 0.5% of the mass of the base material is added with a lactic acid tablet with a bacterial activity of 1 billion CFU / g cocci, spray fermentation at 35°C for 25h, adjust pH8, add trypsin with an enzyme activity of 500,000 U / g, which is 1% of the mass of the base material, and perform enzymolysis at 37°C for 40min, then add nattokinase with an enzyme activity of 10,000 U / g, which is 1% of the mass of the base material, and perform enzymolysis at 55°C for 90min, adjust pH7.5, sieve through 100 mesh, take the sieve, centrifuge at 5000r / min for 40min, take the solid, and dry it at 75°C to obtain a composite extract of plantain and selfhealing.

[0084] The preparation method of the composite extract of purslane and sweet potato vine comprises the following steps:

[0085] According to the mass ratio of purslane: sweet potato vine leaves=1:15, the whole plant of purslane and the sweet potato vine leaves are mixed and crushed to obtain a base material, 10 times the mass of water of the base material is added to the base material, 5% of the mass of glucose, 0.8% of the mass of peptone, and 0.05% of the mass of potassium hydrogen phosphate are added to the base material, the pH is adjusted to 6.0, 1% of the mass of the base material Lactobacillus cellobiose with a bacterial activity of 3 billion CFU / g is added, and the base material is sprayed and fermented at 30°C for 20 hours, the pH is adjusted to 6.4, and the base is added. 0.5% of the base material mass of rumen Bacteroides with an activity of 5 billion CFU / g was anaerobically fermented at 40°C for 15h, the pH was adjusted to 6.2, 0.5% of the base material mass of snail protease with an enzyme activity of 5,000 U / g was added, and enzymolysis was carried out at 35°C for 50min, then the pH was adjusted to 8.4, 1% of the base material mass of trypsin with an enzyme activity of 100,000 U / g was added, and enzymolysis was carried out at 37°C for 60min, the pH was adjusted to 7.2, sieved with 200 mesh, the sieve was taken, and the material was centrifuged at 5000r / min for 40min. The solid was taken and dried at 72°C to obtain a composite extract of purslane and sweet potato vine.

[0086] The preparation method of the Eucommia ulmoides leaf extract comprises the following steps:

[0087] The eucommia leaves are crushed, water with an activity of 10 times the mass of the eucommia leaves is added, the pH is adjusted to 4.8, hemicellulase with an enzyme activity of 50,000 U / g in an amount of 0.8% by mass of the eucommia leaves is added, enzymolysis is performed at 52° C. for 4 hours, the pH is adjusted to 7.2, bromelain with an enzyme activity of 500,000 U / g in an amount of 0.8% by mass of the eucommia leaves is added, enzymolysis is performed at 50° C. for 70 minutes, the enzyme is inactivated at 82° C. for 10 minutes, the pH is adjusted to 8.1 after cooling to room temperature, nattokinase with an enzyme activity of 50,000 U / g in an amount of 1% by mass of the base material is added, enzymolysis is performed at 52° C. for 70 minutes, the pH is adjusted to 7.2, 200 meshes are sieved, the sieve is taken, centrifuged at 5000 r / min for 30 minutes, the solid is taken, and dried at 72° C. to obtain an eucommia leaf extract.

[0088] The method for preparing the above-mentioned immune-enhancing feed for pigs containing natural plant extracts comprises the following steps:

[0089] S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A;

[0090] S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B;

[0091] S3: Mix the mixture A and the mixture B to obtain feed.

[0092] Comparative Example 1

[0093] The plantain and selfheal composite extract is not added to the feed, and the content of the plantain and selfheal composite extract is replaced by corn flour; other methods and parameters are the same as in Example 1.

[0094] Comparative Example 2

[0095] The composite extract of purslane and sweet potato vine leaves is not added to the feed, and the content of the composite extract of purslane and sweet potato vine leaves is replaced by corn flour; other methods and parameters are the same as in Example 1.

[0096] Comparative Example 3

[0097] No eucommia leaf extract was added to the feed, and the content of the eucommia leaf extract was replaced by corn flour; other methods and parameters were the same as in Example 1.

[0098] Comparative Example 4

[0099] No plantain and selfheal composite extract or eucommia leaf extract is added to the feed; the plantain and selfheal composite extract and eucommia leaf extract are replaced by corn flour; other methods and parameters are the same as in Example 1.

[0100] Comparative Example 5

[0101] In the preparation method of the composite extract of plantain and selfheal, Lactobacillus cellobiose is not added during fermentation, and nattokinase is not used for enzymatic hydrolysis; other methods and parameters are the same as those in Example 1.

[0102] Comparative Example 6

[0103] In the preparation method of the composite extract of purslane and sweet potato vine, rumen Bacteroides fermentation is not used, and snail protease is not used for enzymatic hydrolysis; other methods and parameters are the same as those in Example 1.

[0104] Comparative Example 7

[0105] In the preparation method of the eucommia leaf extract, nattokinase is not used for enzymolysis; other methods and parameters are the same as those in Example 1.

[0106] Experiment 1: Mouse immunity experiment was carried out on the composite extracts of plantain and selfheal, composite extracts of purslane and sweet potato vine leaves, and extracts of eucommia leaves prepared in the above embodiments and comparative examples 5, 6, and 7.

[0107] Test samples: compositions of plantain and selfheal composite extract, purslane and sweet potato vine leaf composite extract, and eucommia leaf extract prepared in each embodiment and comparative example 5, comparative example 6, and comparative example 7, with a mass ratio of plantain and selfheal composite extract: purslane and sweet potato vine leaf composite extract: eucommia leaf extract = 1:1:0.2.

[0108] Preparation of sample solution: Weigh each of the above test samples and prepare a sample solution containing the test sample at a concentration of 700 mg / mL using distilled water.

[0109] A number of healthy SPS grade mice were selected; after one week of adaptive feeding, the mice were randomly divided into 13 groups, with 10 mice in each group. Among them, 12 groups of mice were gavaged with each sample solution, 2mL / kg of mouse body weight each time; the other group was a blank group, and the mice were gavaged with sterile water, 2mL / kg of mouse body weight each time. Each group was gavaged three times a day for 30 consecutive days. 12 hours after the last administration of the mice, the spleen of the mice was placed in a plate containing sterile Han k's solution, and the spleen of the mice was ground to make a single cell suspension; each spleen cell suspension was added to two wells of a 24-well culture plate, 1mL per well, one well was added with 75μL ConA solution, and the other well was used as a control, and 5% CO was placed. 2, cultured in a 37°C incubator for 72 hours. 4 hours before the end of the culture, 0.7 mL of supernatant was gently aspirated from each well, and 0.7 mL of RPMI 1640 culture medium without calf serum was added, and 50 μL / well of MTT (5 mg / mL) was added, and the culture was continued for 4 hours. After the end of the culture, 1 mL of acidic isopropanol was added to each well, and the mixture was blown and mixed to completely dissolve the purple crystals, and then dispensed into a 96-well culture plate, and 3 parallel wells were made for each well. The optical density (OD) was measured at 570 nm using an ELISA reader. The results are shown in Table 1 below.

[0110] Table 1 Lymphocyte proliferation ability (average OD value)

[0111] Group OD value Group OD value Blank group 0.124 Comparative Example 5 0.142 Example 1 0.163 Comparative Example 6 0.150 Example 2 0.159 Comparative Example 7 0.153 Example 3 0.168 Example 4 0.161 Example 5 0.166

[0112] From the above results, it can be seen that the compositions of Examples 1 to 5 have a good effect of promoting lymphocyte proliferation and help improve immunity. From the results of Comparative Examples 5 to 7, it can be seen that by changing the preparation methods of the composite extract of plantain and Prunella vulgaris, the composite extract of purslane and sweet potato vine leaves, and the extract of Eucommia ulmoides leaves, the components are different, and the effect on lymphocyte proliferation is significantly reduced, affecting the immune effect.

[0113] Experiment 2: Experiment on the effect of feeds prepared in the above embodiments and comparative examples on the immune function of piglets

[0114] Basic feed: 64.2 parts corn meal, 20 parts soybean meal, 10 parts wheat bran meal, 2.5 parts fish meal, 0.5 parts calcium hydrogen phosphate, and 2 parts whey powder.

[0115] Experimental subjects: 65 pregnant sows and their piglets. The pregnant sows were divided into 13 groups, with 5 sows in each group.

[0116] One month before the expected date of delivery, the sows in each group were fed with the basic feed until delivery. After delivery, one group continued to feed the sows with the basic feed as a blank control group; the remaining 12 groups were fed with the feeds of each embodiment and each comparative ratio as experimental groups. During the experiment, the temperature, ventilation, light and other conditions of each group were kept consistent, and food and water were freely available.

[0117] Test index detection:

[0118] On the 14th and 21st day after the piglets were born, 5 piglets were selected for fasting anterior vena cava blood collection of 5 mL, and the blood was centrifuged at 4°C, 3000r / min for 5 min. The upper serum was taken and the levels of serum antibodies (serum immunoglobulins) IgG, IgA, and IgM were detected using ELISA kits. The test results are shown in Table 2 below.

[0119] Table 2 Test results of serum immunoglobulin content in piglets (average g / L)

[0120]

[0121] From the above results, it can be seen that the feeds of Examples 1 to 5 can effectively increase the content of serum immunoglobulins and promote the improvement of immunity. From the results of Comparative Examples 1 to 4, it can be seen that the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, and the extract of Eucommia ulmoides leaves all have a good effect of promoting the production of serum immunoglobulins. The composite extract of plantain and selfheal and the extract of Eucommia ulmoides leaves can greatly promote the production of immunoglobulins. From the results of Comparative Examples 5 to 7, it can be seen that changing the preparation methods of the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine, and the extract of Eucommia ulmoides leaves, respectively, will change the product composition and have almost no effect on promoting the production of serum immunoglobulins.

[0122] Experiment 3: During Experiment 2, the diarrhea rates of sows and piglets were observed:

[0123] Diarrhea rate = total diarrhea times / total fecal excretion times × 100%; the test results are shown in Table 3 below.

[0124] Table 3 Statistical results of diarrhea rate

[0125] Group Diarrhea rate% Group Diarrhea rate% Example 1 1.36 Comparative Example 1 3.04 Example 2 1.67 Comparative Example 2 3.07 Example 3 1.21 Comparative Example 3 3.12 Example 4 1.54 Comparative Example 4 2.88 Example 5 1.30 Comparative Example 5 3.06 Blank control group 3.89 Comparative Example 6 3.08 Comparative Example 7 3.17

[0126] From the above results, it can be seen that the feeds of Examples 1 to 5 can enhance immunity, improve intestinal health, and reduce diarrhea. From the results of Comparative Examples 1 to 4, it can be seen that the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, and the extract of Eucommia ulmoides leaves all have good effects of enhancing immunity, improving intestinal health, and reducing diarrhea. From the results of Comparative Examples 5 to 7, it can be seen that changing the preparation methods of the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine, and the extract of Eucommia ulmoides leaves, respectively, will change the product composition, reduce the ability to enhance immunity, be easily affected by harmful bacteria, and have poor anti-diarrhea ability.

Claims

1. An immune enhancing feed for pigs containing natural plant extracts, characterized in that: The feed includes the following raw materials in parts by weight: 0.5 to 1.5 parts of plantain and selfheal compound extract, 0.5 to 1.5 parts of purslane and sweet potato vine leaf compound extract, 0.1 to 0.3 parts of eucommia leaf extract, 60 to 65 parts of corn flour, 15 to 25 parts of soybean meal, 3 to 17 parts of wheat bran powder, 0 to 5 parts of fish meal, 0 to 1 part of calcium hydrogen phosphate, and 0 to 5 parts of whey powder; The plantain and selfheal composite extract is a product obtained by fermenting the whole plant of plantain and the whole plant of selfheal in sequence with Lactobacillus cellobiose and Pediococcus acidilactici, and then enzymolyzing them step by step with trypsin and nattokinase in sequence; The purslane and sweet potato vine composite extract is a product obtained by fermenting the whole purslane plant and sweet potato vine leaves with cellobiose lactobacillus and rumen bacteroides in sequence, and then enzymolyzing them with snail protease and trypsin in sequence; The eucommia leaf extract is a product obtained by sequentially enzymolyzing the eucommia leaves with hemicellulase, bromelain and nattokinase.

2. The immune enhancing feed for pigs containing natural plant extracts according to claim 1, characterized in that: The feed also includes 0 to 4 parts of premix, and the premix includes vitamins, minerals, antioxidants, mildew inhibitors, and flavoring agents.

3. The immune enhancing feed for pigs containing natural plant extracts according to claim 1, characterized in that: The preparation method of the plantain and selfheal composite extract comprises the following steps: According to the mass ratio, plantain: selfheal = (5-10): (3-5), the whole plant of plantain and the whole plant of selfheal are mixed and crushed to obtain a base material, 10-15 times the mass of water of the base material is added to the base material, 3%-5% of glucose by mass of the base material, 0.3%-0.8% of peptone by mass of the base material, and 0.03%-0.05% of dipotassium hydrogen phosphate by mass of the base material are added, the pH is adjusted to 6.0-6.4, 1%-2.5% of Lactobacillus cellobiose by mass of the base material and 0.5%-1% of Pediococcus acidilactici by mass of the base material are added, and spray fermentation is carried out at 30° C.-35° C. for 25 h-30 h, the pH is adjusted to 8-8.5, and 0.5%-1% of trypsin by mass of the base material is added, and enzymolysis is carried out at 35° C.-37° C. for 40 m in~60min, then add 0.5%~1% of nattokinase by weight of the base material, carry out enzymolysis at 45℃~55℃ for 60min~90min, adjust the pH to 6.5~7.5, sieve, take the sieve, centrifuge, take the solid, dry, and obtain the plantain and selfheal composite extract.

4. The immune enhancing feed for pigs containing natural plant extracts according to claim 1, characterized in that: The preparation method of the purslane and sweet potato vine composite extract comprises the following steps: According to the mass ratio, purslane: sweet potato vine leaves = (1-3): (5-15), the whole purslane plant and the sweet potato vine leaves are mixed and crushed to obtain a base material, 10 to 15 times the mass of water of the base material is added to the base material, 3% to 5% of glucose by mass of the base material, 0.3% to 0.8% of peptone by mass of the base material, and 0.03% to 0.05% of dipotassium hydrogen phosphate by mass of the base material are added, the pH value is adjusted to 6.0 to 6.4, 1% to 2.5% of Lactobacillus cellobiose by mass of the base material is added, and the mixture is sprayed and fermented at 30°C to 35°C for 20h to 24h, the pH value is adjusted to 6.0 to 7.0, and 0.5% to 1% of the mass of the base material Bacteroides rumensis is fermented anaerobically at 39°C to 41°C for 15h to 20h, the pH is adjusted to 5.5 to 6.5, 0.5% to 1.5% of the mass of the base material snail protease is added, enzymolysis is carried out at 35°C to 40°C for 50min to 80min, then the pH is adjusted to 8 to 8.5, 0.5% to 1% of the mass of the base material trypsin is added, enzymolysis is carried out at 35°C to 37°C for 40min to 60min, the pH is adjusted to 6.5 to 7.5, sieving, taking the sieve, centrifuging, taking the solid, and drying to obtain a composite extract of purslane and sweet potato vine.

5. The immune enhancing feed for pigs containing natural plant extracts according to claim 1, characterized in that: The preparation method of the Eucommia ulmoides leaf extract comprises the following steps: Take eucommia leaves and grind them, add water 8 to 10 times the mass of the eucommia leaves, adjust the pH to 4.5 to 5.5, add hemicellulase 0.5 to 2% by mass of the eucommia leaves, perform enzymolysis at 50 to 55° C. for 3 hours to 4 hours, adjust the pH to 6.5 to 8.0, add bromelain 0.5 to 2% by mass of the eucommia leaves, perform enzymolysis at 50 to 55° C. for 50 to 80 minutes, inactivate the enzyme at 80 to 85° C. for 10 to 15 minutes, cool to room temperature, adjust the pH to 8 to 8.5, add nattokinase 0.5 to 1% by mass of the base material, perform enzymolysis at 45 to 55° C. for 60 to 90 minutes, adjust the pH to 6.5 to 7.5, sieve, take the sieve, centrifuge, take the solid, and dry to obtain the eucommia leaf extract.

6. The immune enhancing feed for pigs containing natural plant extracts according to claim 3, characterized in that: The bacterial activity of the cellobiose Lactobacillus is 1 billion CFU / g to 5 billion CFU / g; the bacterial activity of the acidilactic Pediococcus is 1 billion CFU / g to 5 billion CFU / g; the enzyme activity of the trypsin is 100,000 U / g to 500,000 U / g; and the enzyme activity of the nattokinase is 10,000 U / g to 100,000 U / g.

7. The immune enhancing feed for pigs containing natural plant extracts according to claim 4, characterized in that: The bacterial activity of the cellobiose Lactobacillus is 1 billion CFU / g to 3 billion CFU / g; the bacterial activity of the rumen Bacteroides is 1 billion CFU / g to 5 billion CFU / g; the enzymatic activity of the snail protease is 5,000 U / g to 10,000 U / g; and the enzymatic activity of the trypsin is 100,000 U / g to 300,000 U / g.

8. The immune enhancing feed for pigs containing natural plant extracts according to claim 5, characterized in that: The enzymatic activity of the hemicellulase is 50,000 U / g to 100,000 U / g; the enzymatic activity of the bromelain is 100,000 U / g to 500,000 U / g; and the enzymatic activity of the nattokinase is 20,000 U / g to 100,000 U / g.

9. The immune enhancing feed for pigs containing natural plant extracts according to claim 3, 4 or 5, characterized in that: The mesh number of the sieving sieve is 100-200 mesh; the rotation speed of the centrifugal separation is 3000r / min-6000r / min, and the time of the centrifugal separation is 20min-40min; the drying temperature is 65°C-75°C.

10. The method for preparing the immune enhancing feed for pigs containing natural plant extracts according to claim 1, characterized in that: The method for preparing the feed comprises the following steps: S1: premixing the composite extract of plantain and selfheal, the composite extract of purslane and sweet potato vine leaves, the extract of eucommia leaves and calcium hydrogen phosphate according to weight proportions to obtain a mixture A; S2: premix corn flour, soybean meal, wheat bran powder, fish meal and whey powder according to weight proportions to obtain a mixture B; S3: Mix the mixture A and the mixture B to obtain feed.

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