Preparation method of cordyceps sinensis mycelium powder rich in cubilose peptide and elastin peptide

Abstract

The invention relates to the field of health supplements, and discloses a preparation method of cordyceps sinensis hypha powder rich in cubilose peptide and elastin peptide, which comprises the following steps: carrying out superfine grinding on the cordyceps sinensis hypha powder to obtain powder with the particle size of 10-50 microns; mixing the obtained cordyceps sinensis mycelium powder with cubilose peptide powder, elastin peptide, small molecule peptide of gadus and the like and cubilose acid to form mixed powder; carrying out wet granulation on the mixed powder, and adding 1%-3% of a binding agent to form the mixed powder; and carrying out fluidized bed drying on the granulated particles at the temperature of 60-80 DEG C to obtain dry particles with the water content of less than or equal to 7.0%. The stability of the nutrition powder is remarkably enhanced by adopting vacuum packaging and the aluminum foil composite bag, and external oxygen and moisture are effectively isolated. The packaging protects active ingredients such as cordycepin, protein peptide and the like in the nutrition powder, and prevents oxidation or wet decomposition, so that the shelf life is prolonged, and the efficacy of the product is maintained.

Description

Technical Field

[0001] The invention relates to the field of health supplements, in particular to a method for preparing cordyceps mycelium powder rich in bird's nest peptides and elastin peptides. Background Art

[0002] Cordyceps sinensis, scientifically known as Cordyceps sinensis, is a precious Chinese herbal medicine that has long been used to boost immunity and enhance human health. With the advancement of modern technology, the active ingredients of Cordyceps sinensis, such as cordycepin and polysaccharides, can be converted into nutritional powders through steps such as crushing, extraction and drying for wider application and consumption. These nutritional powders are widely used in health products and nutritional supplements, providing the health benefits of traditional medicine in a convenient form.

[0003] At present, the existing methods for preparing Cordyceps sinensis mycelium powder mainly include mechanical crushing and low-temperature drying technology to maintain the integrity of its biologically active ingredients. These methods can effectively release beneficial ingredients from the raw materials, but auxiliary substances such as stabilizers and preservatives are often added during the process to extend the shelf life of the product. Although these traditional technologies can meet market demand to some extent, the processing steps usually involved may lead to partial loss of nutrients or reduced activity, especially during long-term storage and transportation.

[0004] Although the existing technology can provide basic Cordyceps sinensis mycelium powder, there are still several significant deficiencies. First, the traditional preparation method has limited effect in maintaining the stability of ingredients, and it is easy to cause the degradation of active ingredients due to factors such as oxidation or hygrolysis, thereby affecting the long-term use effect and safety of the product. Secondly, the existing products perform poorly in terms of solubility, and consumers may encounter problems with rapid dissolution when using them, which affects the convenience of use and immediate effect of the product. Finally, there is a lack of an effective mechanism to promote the absorption of ingredients, so that although rich nutrients are provided, the proportion actually absorbed and utilized by the human body is not ideal. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides. The present invention significantly improves the stability of Cordyceps sinensis mycelium powder by using advanced vacuum packaging and aluminum foil composite bag technology, reducing oxidation and hygrolysis problems. The innovative addition of antioxidants and prebiotic fibers optimizes solubility and bioavailability, ensuring rapid absorption and significant health benefits.

[0006] To achieve the above objectives, the present invention is implemented by the following technical scheme: A method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides comprises the following steps:

[0007] Ultrafine grinding of Cordyceps sinensis mycelium powder to obtain powder with a particle size of 10 microns to 50 microns;

[0008] The obtained cordyceps mycelium powder is mixed with bird's nest peptide powder, elastin peptide, first-class cod fish small molecule peptide and bird's nest acid to form a mixed powder;

[0009] Wet granulate the mixed powder and add 1% to 3% of a binder to form it;

[0010] The granulated particles are subjected to fluidized bed drying at a temperature of 60° C. to 80° C. to obtain dry particles having a moisture content of ≤7.0%;

[0011] The dried particles are tableted and then packaged to obtain Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides.

[0012] Preferably, before ultrafine grinding, the Cordyceps sinensis mycelium powder is subjected to enzyme treatment, and the enzyme treatment comprises:

[0013] Treating Cordyceps sinensis mycelium powder with a mixed solution of cellulase and protease;

[0014] The enzyme addition amount is 0.1% to 0.5% of the total weight of the raw materials, the reaction temperature is 45° C. to 55° C., the pH is 4.0 to 6.0, and the reaction time is 1 to 4 hours.

[0015] Preferably, the formula of the mixed powder comprises:

[0016] Cordyceps sinensis mycelium powder 0.5% to 2%;

[0017] First-class cod small molecule peptide 85% to 95%;

[0018] Bird's nest peptide 1%~3%;

[0019] Elastin peptide 3% to 7%;

[0020] Bird's nest acid 2%~5%.

[0021] Preferably, the mixed powder further comprises microencapsulation treatment of the mixed powder, and the microencapsulation treatment is:

[0022] Use gum arabic as a wrapping material;

[0023] Using spray drying technology, the air inlet temperature is 180℃~220℃, and the air outlet temperature is 80℃~100℃;

[0024] The liquid flow rate is 1-3 mL / min, and a microencapsulated mixed powder is obtained.

[0025] Preferably, the fluidized bed drying includes the following operating parameters and conditions:

[0026] The air inlet temperature of the dryer was set at 70°C to ensure that the protein peptide components would not be denatured by high temperature;

[0027] The drying time was adjusted to 45 minutes to optimize the drying process and ensure uniform removal of moisture from the pellets.

[0028] During the drying process, the air flow velocity in the bed is maintained at 2.0m / s to 3.5m / s to promote uniform drying of the particles and prevent particle aggregation;

[0029] After drying, the final moisture content of the particles was confirmed to be controlled between 3% and 5% by an online humidity detector.

[0030] Preferably, the tablets in the tableting process are subjected to a surface coating treatment, and the surface coating treatment comprises:

[0031] Use natural polymer materials such as gelatin or chitosan;

[0032] The coating thickness is 0.5mm to 1.5mm, ensuring that the tablets gradually release the active ingredients in the body.

[0033] Preferably, the specific operation of tableting includes:

[0034] The pressure is controlled at 5 to 15 tons;

[0035] The tableting time is 1 to 5 seconds.

[0036] Preferably, the fluidized bed drying further comprises:

[0037] The dried granules are sieved to screen out granules with a size greater than 500 microns and less than 150 microns, and granules with a size of 150 microns to 500 microns are retained for tableting and packaging.

[0038] Preferably, the packaging adopts vacuum packaging technology, uses aluminum foil composite bags, and the storage conditions of the packaged products are:

[0039] The temperature is controlled at 15℃~25℃;

[0040] Relative humidity shall not exceed 50%.

[0041] Preferably, the wet granulation comprises the following additives:

[0042] Add 0.2% to 1% antioxidants and 0.5% to 2% prebiotic fiber to improve the antioxidant capacity and intestinal health benefits of the product;

[0043] The pellets were granulated by a granulator with an extrusion speed set at 70 rpm, and a vibrating screen was used to ensure that the diameter of the obtained pellets was uniformly between 100 and 300 microns to ensure uniform distribution of the pellets and a suitable taste;

[0044] After granulation is completed, let it stand for 10 to 20 minutes to allow the particles to further combine and stabilize under natural conditions.

[0045] The present invention provides a method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides. It has the following beneficial effects:

[0046] 1. The present invention significantly improves the stability of the nutritional powder by using a vacuum packaging method combined with an aluminum foil composite bag, effectively isolates the intrusion of external oxygen and moisture, and protects sensitive ingredients from environmental factors. This ensures the stability of active ingredients such as cordycepin and protein peptides during long-term storage, avoids the reduction of activity due to oxidation or hygrolysis, prolongs the shelf life of the product and maintains its efficacy.

[0047] 2. The present invention not only improves the antioxidant capacity of the product by innovatively adding antioxidants and prebiotic fibers to the nutritional powder, but also enhances its anti-inflammatory properties and promotes intestinal health. This design enables the product to exert more comprehensive health benefits in the consumer's body. In addition, the synergistic effect of these additives helps to improve the absorption efficiency of nutrients, so that consumers can more effectively utilize the nutrients in the product.

[0048] 3. The present invention improves the solubility of the nutritional powder by optimizing the material processing flow and the additive ratio. This improvement ensures that the product can dissolve quickly and completely after adding water without residual particles, thereby providing consumers with a smoother taste and a more convenient use experience. The fast dissolution property allows consumers to easily take in the required nutrition in a short time, while also optimizing the release and absorption speed of the nutrients, enhancing the immediate efficacy of the product. DETAILED DESCRIPTION

[0049] The following will be combined with the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] The embodiment of the present invention provides a method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides, comprising the following steps:

[0051] The Cordyceps sinensis mycelium powder is ultrafinely ground to obtain a powder with a particle size of 10 to 50 microns. Ultrafine grinding is a physical method that uses mechanical force to break the Cordyceps sinensis mycelium powder into micron-level particles. This technology uses high-speed rotating blades or ball mills to crush large particles into finer particles. The reduction in particle size can significantly increase the surface area of ​​the powder, thereby increasing the contact area with other substances, improving mixing uniformity and bioavailability, and thus helping to increase the concentration and biological activity of the active ingredients in the final product;

[0052] The obtained cordyceps mycelium powder is mixed with bird's nest peptide powder, elastin peptide, first-class cod small molecule peptide and bird's nest acid to form a mixed powder. The preparation of the mixed powder is to form a complex with consistent properties by evenly mixing different ingredients. In this process, the various powder ingredients are evenly mixed by mechanical stirring to achieve uniform distribution of the ingredients, thereby ensuring the uniformity of each active ingredient in the final product, helping to ensure the quality and functionality of the product, so that the nutrients can have a better effect when consumed;

[0053] Wet granulation is performed on the mixed powder, and 1% to 3% of a binder is added to form it. Wet granulation is to convert powdered materials into granules by adding an appropriate amount of a binder to facilitate subsequent processing and application. The binder acts as an adhesive in this process, helping to form a strong bond between powder particles. Granulation can significantly improve the fluidity and compressibility of the powder, facilitate subsequent drying and tableting, and reduce dust generation, thereby improving the safety of the production process.

[0054] The granulated particles are dried in a fluidized bed at a temperature of 60°C to 80°C to obtain dry particles with a moisture content of ≤7.0%. Fluidized bed drying is an efficient drying technology that uses hot air to quickly evaporate the moisture in wet particles to obtain dry particles. In the fluidized bed, hot air passes through the particle layer from the bottom to the top, so that the particles are suspended in the air flow, similar to a boiling state. This can greatly increase the contact area between the particles and the hot air and improve the drying efficiency. This drying method can quickly and evenly remove moisture, reduce thermal degradation of heat-sensitive substances, and ensure the quality and stability of the particles;

[0055] The dried particles are tableted and packaged to obtain Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides. Tableting is the process of pressing the dried particles into tablets by pressure, which can facilitate the precise control and use of dosage. Packaging is to protect the stability and safety of the product during storage and transportation. The tableting and packaging steps ensure the long-term stability of the product and its easy-to-carry form, making the product more competitive and acceptable to consumers in the market.

[0056] In a preferred embodiment of the present invention, the Cordyceps sinensis mycelium powder is subjected to enzyme treatment before ultrafine grinding, and the enzyme treatment comprises:

[0057] The Cordyceps sinensis mycelium powder is treated with a mixed solution of cellulase and protease. Cellulase and protease are two commonly used biocatalysts that can specifically decompose the cellulose and protein structures in Cordyceps sinensis. Cellulase mainly acts on plant cellulose to help release the active ingredients in the cells; while protease can decompose protein chains and release small molecule peptides or amino acids;

[0058] The enzyme addition amount is 0.1% to 0.5% of the total weight of the raw materials, the reaction temperature is 45°C to 55°C, the pH is 4.0 to 6.0, and the reaction time is 1 to 4 hours. The cordyceps mycelium powder and the mixed enzyme solution are mixed in a reaction container, and the reaction temperature is set at 45°C to 55°C, which is the optimal activity temperature range of most cellulases and proteases. The pH value is adjusted to 4.0 to 6.0, which is suitable for the activity of most enzymes. The reaction time is controlled to 1 to 4 hours and is adjusted according to the actual observed reaction progress and expected effects.

[0059] After the enzyme reaction is completed, the enzyme can be inactivated by heating to 80°C for 10 minutes to prevent the enzyme from continuing to work. In addition, the pH is appropriately adjusted to neutral to protect the product from acid and alkali damage. Through the above steps, enzyme treatment can effectively destroy the complex structure in Cordyceps mycelium and increase the release of active ingredients, thereby improving the biological activity and nutritional value of the product. In addition, enzyme treatment also helps to improve the solubility and taste of the final product, making the nutrients easier to be absorbed and utilized by the human body. This step is a key link in improving the quality and effect of Cordyceps mycelium powder, and helps to produce high-efficiency nutritional health products.

[0060] In a preferred embodiment of the present invention, the formula of the mixed powder comprises:

[0061] Cordyceps sinensis mycelium powder 0.5% to 2%. This ratio of cordyceps sinensis mycelium powder can ensure that the main active ingredients of the product are sufficient and stable, and can ensure that the product contains enough bioactive substances, which is of great help in enhancing human immunity and improving physical performance;

[0062] Bird's nest peptide powder 1% to 3%. Bird's nest peptide is a small molecule peptide obtained by hydrolysis of bird's nest. It is easily absorbed by the human body and has the effects of nourishing yin and moistening the lungs and improving immunity. The addition of bird's nest peptide not only improves the nutritional value of nutrient powder, but also may help improve consumers' skin hydration and anti-aging ability. It is a high-end ingredient in modern functional foods;

[0063] Elastin peptide 3% to 7%. Elastin peptide is a highly effective skin elasticity promoter that can promote the production of human collagen and is beneficial to skin health and joint function. The addition of this ingredient makes the nutritional powder helpful in improving skin elasticity and smoothness, and is suitable for consumers who are concerned about beauty and anti-aging.

[0064] 85% to 95% of first-class cod small molecule peptides. First-class cod small molecule peptides have high bioavailability and safety, and can promote the body's own collagen synthesis. The addition of this proportion of first-class cod small molecule peptides can help improve skin texture and elasticity, while supporting joint and bone health, which is very suitable for middle-aged and elderly consumers and beauty enthusiasts;

[0065] 2% to 5% of bird's nest acid can regulate the immune system and help the body resist the invasion of bacteria. It can bind to the sialic acid on the surface of pathogens to prevent them from attaching to host cells, thereby reducing the possibility of infection. Bird's nest acid also has an antioxidant effect, which can neutralize free radicals, reduce cell damage, and delay skin aging. At the same time, it can also promote the repair and regeneration of skin cells, helping to maintain the elasticity and luster of the skin.

[0066] In a preferred embodiment of the present invention, the microencapsulation process is:

[0067] Using gum arabic as a wrapping material. Gum arabic is a natural gum made from the resin of the Arabica tree. It has excellent emulsifying and film-forming properties. As a wrapping material, it can effectively seal the active ingredients and form a protective layer to prevent oxidation, moisture or heat damage. Using gum arabic as a wrapping material not only protects the sensitive ingredients in the nutritional powder from the external environment, but also improves the solubility and taste of the final product;

[0068] Using spray drying technology, the air inlet temperature is 180℃~220℃, and the air outlet temperature is 80℃~100℃;

[0069] The liquid flow rate is 1-3 mL / min to obtain a microencapsulated mixed powder. Spray drying is a process of converting a liquid mixture into powder through rapid drying technology. In this process, the mixed liquid is sprayed into fine droplets through a high-pressure or air-assisted nozzle, and the water is rapidly evaporated when exposed to a high-temperature airflow to form dry particles. The inlet air temperature can ensure rapid evaporation of the water in the droplets, and the outlet air temperature can prevent the nutrients from being degraded due to high temperature. The rough liquid flow rate can ensure uniform droplet formation and efficient drying process, thereby rapidly producing microencapsulated powder, maintaining the integrity of the active ingredients, and greatly improving the fluidity and stability of the product, extending the shelf life.

[0070] In a preferred embodiment of the present invention, fluidized bed drying includes the following operating parameters and conditions:

[0071] The air inlet temperature of the dryer is set at 70°C to ensure that the protein peptide components are not denatured due to high temperature. This air inlet temperature is a relatively low temperature setting, which is sufficient to quickly evaporate water without causing thermal damage to the protein peptide components. This temperature can be appropriately adjusted according to the properties and sensitivity of the actual material, thereby effectively protecting the structure and function of the protein peptide components and ensuring the high quality of the final product;

[0072] The drying time was adjusted to 45 minutes to optimize the drying process and ensure uniform removal of moisture from the granules. This time length was sufficient to allow most of the moisture to evaporate from the granules while avoiding material loss or quality reduction due to over-drying. This not only ensured drying efficiency, but also helped maintain the integrity and uniformity of the granules, thereby ensuring consistent product quality and effect.

[0073] During the drying process, the air flow velocity in the bed is maintained at 2.0m / s to 3.5m / s to promote uniform drying of the particles and prevent particle aggregation. This velocity range is sufficient to maintain a good fluidization state of the particles and avoid dead zones or particle aggregation in the bed, thereby significantly improving the drying uniformity and efficiency, while preventing adhesion and aggregation between particles, ensuring the quality and stability of the product;

[0074] After drying, the final moisture content of the granules is confirmed to be controlled between 3% and 5% by an online humidity detector. After drying, the moisture content of the granules is monitored in real time by an online humidity detector to ensure that it is controlled between 3% and 5%. This level of moisture content is sufficient to maintain the stability of the product without causing microbial growth, thereby improving the storage stability and shelf life of the product, while maintaining the activity and effectiveness of the nutrients, suitable for long-term storage and transportation.

[0075] In a preferred embodiment of the present invention, the tablets in the tableting process are subjected to a surface coating treatment, and the surface coating treatment comprises:

[0076] Use natural polymer materials, such as gelatin or chitosan. Gelatin and chitosan are two commonly used natural polymer materials. They both have good biocompatibility and biodegradability and can be gradually decomposed in the body.

[0077] The coating thickness is 0.5mm to 1.5mm, ensuring that the tablets gradually release the active ingredients in the body. First, the coating material is dissolved in appropriate water or alcohol to prepare a coating liquid, and then the coating liquid is evenly coated on the compressed tablets using spraying or dipping technology, and the thickness is controlled at 0.5mm to 1.5mm. This range is sufficient to provide an effective controlled-release effect without affecting the overall disintegration time of the tablets, thereby ensuring the gradual release of the tablets in the digestive tract and avoiding the rapid release and loss of active ingredients.

[0078] The drying and curing process after coating is a necessary step to ensure that the coating is stably attached to the surface of the tablet. The coated tablets are placed in a drying room or dried with hot air, and the temperature is set at 40℃~60℃ and dried for 6 hours to completely cure the coating and ensure its stability during packaging and storage. By surface coating the tablets, the physical and chemical stability of the tablets can be significantly improved, and their release time in the consumer's body can be extended, thereby improving the therapeutic effect and patient compliance. In addition, the coating can protect the active ingredients from damage in the initial environment of the digestive tract, ensure that the ingredients are released in the best position, and enhance the overall effectiveness of the product.

[0079] In a preferred embodiment of the present invention, the specific operation of tableting includes:

[0080] The pressure is controlled at 5 to 15 tons, using an automatic or semi-automatic tablet press. This setting range is suitable for most nutritional supplements and pharmaceutical tablets, and can provide adequate compaction for tablets of different sizes and ingredients. Before starting large-scale production, test compression is performed to find the best pressure setting to ensure product quality.

[0081] The tableting time is 1 to 5 seconds, which refers to the duration of the material forming a tablet under pressure. Appropriate tableting time can affect the overall quality of the tablet, including its hardness and release performance. The tableting time set in this way is sufficient to fully compact the material in the mold to form a solid tablet, but it is not too compacted to cause damage to the ingredients or difficulty in disintegration, thereby producing consistent and high-quality tablets, ensuring their stability and reliability in the hands of consumers, and ensuring that consumers get the expected health benefits. In addition, optimized tableting parameters can reduce the waste of raw materials in the production process and improve production efficiency, making it more economically feasible.

[0082] In a preferred embodiment of the present invention, fluidized bed drying further comprises:

[0083] The dried particles are screened, and particles with a size greater than 500 microns and less than 150 microns are screened out, and particles between 150 microns and 500 microns are retained for tableting and packaging. After fluidized bed drying, mechanical screening equipment is used for particle size classification. The aperture selection of the screen is set according to the predetermined particle size range: a 150-micron screen is used to filter out particles smaller than this size, and a 500-micron screen is used to filter out particles larger than this size. The separated unqualified particles can be recycled or treated as waste as needed. Qualified particles enter the next step of tableting or direct packaging process. Through screening, it is ensured that all particles used for tableting are of the same size, which directly affects the compression quality and dosage accuracy of the tablets. Particles of uniform size help to obtain a more uniform pressure distribution during the tableting process, thereby improving the structural stability of the tablets. At the same time, particles of appropriate size can better control the disintegration time and drug release of the tablets, ensuring the effectiveness and consistency of the product.

[0084] In a preferred embodiment of the present invention, the packaging adopts vacuum packaging technology, uses aluminum foil composite bags, and the storage conditions of the packaged products are:

[0085] Vacuum packaging creates an air-scarce environment by extracting air from the package, thereby reducing the presence of oxygen and moisture, while aluminum foil composite bags are made of multiple layers of materials, including aluminum foil and plastic layers. This structure provides excellent light, oxygen and moisture barrier properties;

[0086] The temperature is controlled at 15℃~25℃;

[0087] Relative humidity shall not exceed 50%.

[0088] High temperature can accelerate chemical reactions and the growth of microorganisms, while high humidity promotes moisture absorption and the reproduction of microorganisms. These conditions can slow down the rate of chemical reactions and reduce the risk of microbial contamination. By adopting vacuum packaging technology and using aluminum foil composite bags, the shelf life of the product can be significantly extended to prevent the degradation and contamination of nutrients. In addition, it not only protects the product from environmental influences, but also helps maintain the best consumer quality and efficacy of the product. These packaging and storage measures together ensure the overall quality and safety of the product from production to the hands of consumers, and enhance consumer trust and satisfaction.

[0089] In a preferred embodiment of the present invention, the wet granulation comprises the following additives:

[0090] Add 0.2% to 1% antioxidants and 0.5% to 2% prebiotic fibers to improve the product's antioxidant capacity and intestinal health benefits. Antioxidants help prevent the active ingredients in the product from being degraded due to oxidation and extend the product's shelf life. Prebiotic fibers support intestinal health and improve digestion and absorption by promoting the growth of beneficial bacteria. Adding these ingredients not only enhances the product's health function, but also helps maintain the stability of the active ingredients, thereby improving the product's competitiveness in the market and consumer acceptance;

[0091] The granulation is performed by a granulator with an extrusion speed set at 70 rpm, and a vibrating screen is used to ensure that the diameter of the obtained granules is uniformly between 100 and 300 microns to ensure uniform distribution of the granules and a suitable taste. By controlling the extrusion speed of the granulator and using a vibrating screen to screen the granules, granules with consistent specifications can be obtained, which helps to optimize the tablet compression process, improve the disintegration time and drug release of the final product, and thus enhance the consumer experience;

[0092] After granulation is completed, let it stand for 10 to 20 minutes to allow the particles to further combine and stabilize under natural conditions. After granulation is completed, spread the particles on a drying tray and let it stand for 10 to 20 minutes. Ensure that the temperature and humidity of the standing environment are appropriate to avoid moisture or excessive drying of the particles. The standing process helps the natural solidification of the particles, reduces dust generation and particle breakage in subsequent processing, ensures that the tablets are of high quality and stable, and also improves the storage and handling performance of the particles, reducing losses during the production process.

[0093] In order to better understand the present invention, the above contents are described in detail below in conjunction with specific embodiments.

[0094] Example 1: Standard preparation process

[0095] The Cordyceps sinensis mycelium powder is ultrafinely ground to obtain a powder with a particle size of 30 microns.

[0096] Mix the ultra-finely crushed Cordyceps sinensis mycelium powder with the other ingredients in the following proportions:

[0097] Cordyceps sinensis mycelium powder: 1%

[0098] First-class cod small molecule peptide: 90%

[0099] Bird's nest peptide powder: 2%

[0100] Elastin peptides: 5%

[0101] Bird's Nest Acid: 2%

[0102] The mixed powder was wet granulated and 2% gelatin was added as a binder to give a shape.

[0103] The granulated particles were fluidized bed dried at 70° C. for 45 minutes to obtain dry particles with a moisture content of 5%.

[0104] The dried particles were tableted and surface coated with chitosan with a thickness of 0.5 mm.

[0105] The tableted product is packaged in a vacuum package using an aluminum foil composite bag at a storage temperature of 20° C. and a humidity of 45%.

[0106] Example 2: Enhanced Antioxidant and Gut Health Benefits

[0107] Before ultrafine grinding, the Cordyceps sinensis mycelium powder was treated with enzyme, the enzyme addition amount was 0.3%, the reaction temperature was 50°C, the pH value was 5.0, and the reaction time was 2 hours. Then ultrafine grinding was performed to obtain a powder with a particle size of 20 microns.

[0108] The obtained Cordyceps sinensis mycelium powder is mixed with other ingredients in the following proportions:

[0109] Cordyceps sinensis mycelium powder: 1.5%

[0110] First-class cod small molecule peptide: 88%

[0111] Bird's nest peptide powder: 1.5%

[0112] Elastin peptides: 6%

[0113] Bird's Nest Acid: 3%

[0114] The mixed powder was wet granulated, 1% antioxidant and 1% prebiotic fiber were added as additives, and the binder was 3% gum arabic.

[0115] The pellets were granulated using a granulator at 70 rpm and passed through a vibrating screen to ensure that the particle diameter was 200 μm. The pellets were then allowed to stand for 15 minutes.

[0116] The granulated particles were dried in a fluidized bed at 75°C, and the final moisture content was controlled at 4%.

[0117] The tablets were pressed using a pressure of 12 tons, a tableting time of 4 seconds, and a 1 mm thick gelatin coating.

[0118] Use vacuum packaging and store at 15°C and 50% humidity.

[0119] Example 3: Microencapsulated Cordyceps Mycelium for Enhanced Skin Elasticity and Immune Support

[0120] Directly carry out ultrafine grinding to obtain Cordyceps sinensis mycelium powder with a particle size of 40 microns.

[0121] Mix the ultra-finely crushed Cordyceps sinensis mycelium powder with the other ingredients in the following proportions:

[0122] First-class cod small molecule peptide: 89%

[0123] Bird's nest peptide powder: 1.5%

[0124] Elastin peptides: 5%

[0125] Bird's Nest Acid: 3%

[0126] Cordyceps sinensis mycelium powder: 1%

[0127] The mixed powder was microencapsulated, and gum arabic was used as the encapsulation material. Spray drying technology was used, and the air inlet temperature was set at 200°C, the air outlet temperature was set at 90°C, and the liquid flow rate was 2mL / min to obtain the microencapsulated mixed powder.

[0128] The microencapsulated powder was wet granulated, 2% of a binder was added, and fluidized bed drying was performed at 70° C. for 50 minutes. The final moisture content was controlled at 3%.

[0129] The tableting pressure was controlled at 8 tons, the tableting time was 2 seconds, and a chitosan coating with a thickness of 0.8 mm was performed.

[0130] Use aluminum foil composite bags for vacuum packaging, and the storage conditions are temperature 22°C and humidity 48%.

[0131] Comparative experiment 1: Antioxidant capacity test

[0132] Experimental purpose: To verify the antioxidant effect of the product prepared from the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention, and to compare it with the products currently on the market.

[0133] Experimental setup

[0134] Experimental and control group settings:

[0135] Experimental group: a product prepared using the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention.

[0136] Control group 1: Use a health product on the market that focuses on antioxidant function.

[0137] Control group 2: Use another common antioxidant health product.

[0138] Control group 3: No antioxidant products were used, and only physiological saline was used for treatment.

[0139] Experimental materials and equipment:

[0140] DPPH free radical solution

[0141] Ethanol solution

[0142] Micropipette

[0143] 96-well plate

[0144] Spectrophotometer

[0145] Experimental steps:

[0146] Prepare DPPH solution: prepare 0.1 mM DPPH ethanol solution and place it in a dark environment until use.

[0147] Sample preparation:

[0148] Experimental group: The product of the present invention was dissolved in ethanol at concentrations of 100, 200, 400 and 800 μg / mL.

[0149] Control groups 1 and 2: Take market products A and B respectively and prepare solutions at the same concentration.

[0150] Blank control group: pure ethanol was used.

[0151] Reaction mixing: Add 100 μL of DPPH solution and 100 μL of sample solution of each concentration into a 96-well plate. Set up three replicates for each group of samples.

[0152] Incubation: Incubate in the dark for 30 minutes to ensure completion of the free radical reaction.

[0153] Measure absorbance: Measure the absorbance of the sample at a wavelength of 517 nm on a spectrophotometer.

[0154] Calculate the free radical scavenging rate.

[0155] The comparative experimental data are shown in Table 1:

[0156] Table 1 Product stability test results

[0157] Sample concentration (μg / mL) Experimental Group Control group 1 Control group 2 Control group 3 100 25.3％ 20.5％ 19.7％ 5.2％ 200 42.7％ 35.1％ 34.8％ 7.1％ 400 62.4％ 52.3％ 49.5％ 8.2％ 800 85.2％ 68.4％ 64.9％ 10.3％

[0158] From the data in Table 1, we can get:

[0159] The free radical scavenging ability of this experimental group at different concentrations is better than that of existing products on the market. This shows that the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention has a stronger antioxidant effect and significantly improves the free radical scavenging rate.

[0160] Comparative experiment 2: Skin elasticity improvement effect test

[0161] Experimental purpose: This experiment aims to verify the effect of the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides in improving skin elasticity, and compare it with existing health care products on the market. By measuring skin elasticity indicators, evaluate whether the present invention has significant advantages in improving skin elasticity.

[0162] Experimental setup

[0163] Experimental and control group settings:

[0164] Experimental group: Orally taking the product prepared from the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention daily.

[0165] Control group 1: Use a health care product on the market that focuses on improving skin elasticity.

[0166] Control group 2: Use another health care product whose main function is to improve skin elasticity.

[0167] Control group 3: No health products were used, only placebo was taken.

[0168] Subjects:

[0169] A total of 80 healthy volunteers, aged between 30 and 50 years old, were randomly divided into 4 groups, with 20 people in each group.

[0170] Experimental materials and equipment:

[0171] Cutometer Skin Elasticity Tester

[0172] Recording tables and statistical software

[0173] Experimental steps:

[0174] Baseline measurements: Before the experiment, the skin elasticity of all participants was measured using a Cutometer, and baseline data including skin resilience and overall elasticity were recorded.

[0175] Product Use:

[0176] Experimental group: took the product of the present invention once a day for 12 consecutive weeks.

[0177] Control group 1: took market product C once a day for 12 consecutive weeks.

[0178] Control group 2: took market product D once a day for 12 consecutive weeks.

[0179] Control group 3: took placebo once daily for 12 weeks.

[0180] Regular measurements:

[0181] Cutometer was used to measure the skin elasticity of volunteers in each group every 4 weeks, and the data were recorded at the 4th, 8th and 12th weeks.

[0182] Data collection and analysis: Record and count the skin elasticity data at each time point, and analyze the changing trends of each group at different time points.

[0183] The comparative experimental data are shown in Table 2:

[0184] Table 2 Comparative experimental data of skin elasticity improvement effect

[0185]

[0186]

[0187] From the data in Table 2, we can get:

[0188] The experimental results show that the product prepared from the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention is significantly superior to the two existing health care products and placebo on the market in improving skin elasticity.

[0189] Comparative experiment 3: immune function enhancement test

[0190] Purpose of the experiment: This experiment aims to verify the effect of the product prepared from the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention in improving immune function, and compare it with the existing health care products on the market.

[0191] Experimental setup

[0192] Experimental and control group settings:

[0193] Experimental group: The mouse model orally administered the product prepared from the Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides of the present invention.

[0194] Control group 1: Use a health product on the market that focuses on improving immune function.

[0195] Control group 2: Use another common immune support health product.

[0196] Control group 3: No health care products were used, only physiological saline was used.

[0197] Subjects:

[0198] Eighty healthy mice were randomly divided into 4 groups, with 20 mice in each group.

[0199] Experimental materials and equipment:

[0200] Serum collection tube

[0201] Immunoglobulin ELISA Kit

[0202] Flow cytometry

[0203] Cell culture equipment

[0204] Experimental steps:

[0205] Baseline measurements: Before the start of the experiment, 5 mice were randomly selected from each group, and blood samples were collected and baseline immunoglobulin levels and lymphocyte activity were measured.

[0206] Product Use:

[0207] Experimental group: Orally take the product of the present invention once a day for 4 consecutive weeks.

[0208] Control group 1: Orally take market product E once a day for 4 consecutive weeks.

[0209] Control group 2: Orally take market product F once a day for 4 consecutive weeks.

[0210] Control group 3: Oral administration of normal saline once a day for 4 consecutive weeks.

[0211] Periodic measurement:

[0212] Five mice were randomly selected from each group every week, and blood samples were collected to measure immunoglobulin levels and lymphocyte activity.

[0213] The changes in immune function of mice in each group were recorded and analyzed.

[0214] End point measurement: At the end of the fourth week, all mice were subjected to endpoint measurement to analyze the changes in overall immune function of each group.

[0215] The comparative experimental data are shown in Table 3 and Table 4:

[0216] Table 3 Comparison of experimental data on IgG levels in mouse serum at different time points

[0217] Time Experimental Group Control group 1 Control group 2 Control group 3 Baseline 230±15 225±18 228±16 229±17 Week 1 255±14 240±17 238±15 230±18 Week 2 280±16 255±19 248±18 233±17 Week 3 315±18 270±20 265±17 235±16 Week 4 340±20 285±19 275±18 237±18

[0218] From the data in Table 3, we can get:

[0219] Throughout the experiment, the IgG level of the experimental group was significantly higher than that of all control groups. The experimental group reached 340 μg / mL at the end of the fourth week, an increase of 48% over the baseline value, while the IgG levels of market products E and F increased to 285 μg / mL and 275 μg / mL, respectively, with relatively small increases. The IgG level of the blank control group showed almost no significant change and remained near the baseline level. This shows that the present invention exhibits a stronger immune enhancement ability.

[0220] Table 4 Comparative experimental data of mouse lymphocyte activity at different time points

[0221]

[0222]

[0223] From the data in Table 4, we can get:

[0224] The lymphocyte activity of the mice in the experimental group reached 65% at the 4th week, which was 20 percentage points higher than the baseline value and significantly higher than the market products E and F. The lymphocyte activity of the blank control group did not change much and remained at the baseline level, indicating that the product of the present invention is more effective in enhancing immune response and improving the body's defense ability.

[0225] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides, characterized in that: The following steps are involved: Ultrafine grinding of Cordyceps sinensis mycelium powder to obtain powder with a particle size of 10 microns to 50 microns; The obtained cordyceps mycelium powder is mixed with bird's nest peptide powder, elastin peptide, first-class cod fish small molecule peptide and bird's nest acid to form a mixed powder; Wet granulate the mixed powder and add 1% to 3% of a binder to form it; The granulated particles are subjected to fluidized bed drying at a temperature of 60° C. to 80° C. to obtain dry particles having a moisture content of ≤7.0%; The dried particles are tableted and then packaged to obtain Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides.

2. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: Before ultrafine grinding, the Cordyceps sinensis mycelium powder is subjected to enzyme treatment, and the enzyme treatment comprises: Treating Cordyceps sinensis mycelium powder with a mixed solution of cellulase and protease; The enzyme addition amount is 0.1% to 0.5% of the total weight of the raw materials, the reaction temperature is 45° C. to 55° C., the pH is 4.0 to 6.0, and the reaction time is 1 to 4 hours.

3. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: The formula of the mixed powder includes: Cordyceps sinensis mycelium powder 0.5% to 2%; First-class cod small molecule peptide 85% to 95%; Bird's nest peptide powder 1%~3%; Elastin peptide 3% to 7%; Bird's nest acid 2%~5%.

4. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 3, characterized in that: The mixed powder further comprises microencapsulation treatment of the mixed powder, wherein the microencapsulation treatment is: Use gum arabic as a wrapping material; Using spray drying technology, the air inlet temperature is 180℃~220℃, and the air outlet temperature is 80℃~100℃; The liquid flow rate is 1-3 mL / min, and a microencapsulated mixed powder is obtained.

5. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: The fluidized bed drying includes the following operating parameters and conditions: The air inlet temperature of the dryer was set at 70°C to ensure that the protein peptide components would not be denatured by high temperature; The drying time was adjusted to 45 minutes to optimize the drying process and ensure uniform removal of moisture from the pellets. During the drying process, the air flow velocity in the bed is maintained at 2.0m / s to 3.5m / s to promote uniform drying of the particles and prevent particle aggregation; After drying, the final moisture content of the particles was confirmed to be controlled between 3% and 5% by an online humidity detector.

6. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: The tablets in the tableting process are subjected to surface coating treatment, and the surface coating treatment comprises: Use natural polymer materials such as gelatin or chitosan; The coating thickness is 0.5mm to 1.5mm, ensuring that the tablets gradually release the active ingredients in the body.

7. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 6, characterized in that: The specific operation of tableting includes: The pressure is controlled at 5 to 15 tons; The tableting time is 1 to 5 seconds.

8. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 5, characterized in that: The fluidized bed drying further comprises: The dried granules are sieved to screen out granules with a size greater than 500 microns and less than 150 microns, and granules with a size of 150 microns to 500 microns are retained for tableting and packaging.

9. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: The packaging adopts vacuum packaging technology, uses aluminum foil composite bags, and the storage conditions of the packaged products are: The temperature is controlled at 15℃~25℃; Relative humidity shall not exceed 50%.

10. The method for preparing Cordyceps sinensis mycelium powder rich in bird's nest peptides and elastin peptides according to claim 1, characterized in that: The wet granulation process includes the following additives: Add 0.2% to 1% antioxidants and 0.5% to 2% prebiotic fiber to improve the antioxidant capacity and intestinal health benefits of the product; The pellets were granulated by a granulator with an extrusion speed set at 70 rpm, and a vibrating screen was used to ensure that the diameter of the obtained pellets was uniformly between 100 and 300 microns to ensure uniform distribution of the pellets and a suitable taste; After granulation is completed, let it stand for 10 to 20 minutes to allow the particles to further combine and stabilize under natural conditions.

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