Medicinal and edible traditional Chinese medicine extract composition for promoting pancreatic beta cell proliferation

Abstract

The invention relates to the field of medicines, in particular to a medicinal and edible traditional Chinese medicine extract composition for promoting pancreatic beta cell proliferation. Natural and non-toxic medicinal and edible medicines are selected, and meanwhile, the proliferation effect of the medicinal and edible composition on pancreatic beta cells is evaluated, so that the efficient medicinal and edible composition for promoting pancreatic beta cell proliferation is obtained. Results show that after the seven medicinal and edible medicines including the lotus leaves, the sophora flowers, the sea-buckthorn, the lalang grass rhizome, the codonopsis pilosula, the raw astragalus membranaceus and the fried endothelium corneum gigeriae galli are jointly applied, pancreas islet beta cell proliferation can be synergistically promoted, pancreas islet beta cell functions can be improved, and diabetes mellitus can be prevented, improved and / or treated.

Description

Technical Field

[0001] The invention relates to the field of medicines, and in particular to a Chinese medicinal extract composition having both medicinal and edible properties and capable of promoting the proliferation of pancreatic beta cells. Background Art

[0002] Diabetes is a group of metabolic diseases characterized by high blood sugar caused by the combined effects of genetic and environmental factors. It is mainly caused by insulin resistance and insulin secretion defects, which lead to metabolic disorders of sugar, fat, protein and other substances. According to statistics from the International Diabetes Federation (IDF), the total number of diabetes patients in the global population aged 20-79 in 2021 is about 537 million, and the number of patients will reach 784 million by 2045.

[0003] Diabetes includes type 1 diabetes (absolute lack of pancreatic β cells that secrete insulin, T1DM) and type 2 diabetes (T2DM) with a large loss of pancreatic β cells. More and more studies have shown that both type 1 and type 2 diabetes ultimately have a pathological mechanism of loss of pancreatic β cells that secrete insulin.

[0004] Diabetes has a slow course and is not cured for a long time. Long-term use of certain non-medicinal and edible drugs may cause nephrotoxicity. In addition, few studies have been conducted on Chinese herbal medicine compositions for the treatment of diabetes that target the repair of pancreatic β cells.

[0005] Traditional Chinese medicine, with its multi-component and multi-target characteristics, has more advantages than Western medicine in the treatment of diabetes. On the one hand, traditional Chinese medicine can comprehensively regulate the body's functions and improve the patient's overall health, rather than just treating symptoms. On the other hand, traditional Chinese medicine can regulate insulin secretion, promote sugar metabolism and other ways to lower blood sugar levels, and its effects are stable. Compared with Western medicine, traditional Chinese medicine has less effect on the liver and kidney function of diabetic patients and has relatively fewer side effects. In addition, traditional Chinese medicine can prevent the occurrence of diabetic complications by improving microcirculation and protecting the kidneys. It can also be personalized according to individual differences such as the patient's constitution and condition to improve the treatment effect.

[0006] The concept of medicine and food being of the same origin is an important part of the concept of traditional Chinese medicine, which emphasizes that food itself has medicinal value and can be used to prevent and treat diseases. Applying traditional Chinese medicine that is of the same origin as medicine to treat diabetes is natural and safe, has a mild effect, and is a comprehensive conditioning method combined with diet, making it more accessible.

[0007] Therefore, it is of great practical significance to provide a Chinese herbal medicine extract composition that is both medicinal and edible and can promote the proliferation of pancreatic β cells. Summary of the invention

[0008] In view of this, the present invention aims to provide a Chinese medicine composition that is both a medicine and a food and can promote the proliferation of pancreatic β cells. The composition has the effect of synergistically promoting the proliferation of pancreatic β cells, can improve the function of pancreatic β cells, and is particularly suitable for diabetic patients.

[0009] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0010] In a first aspect, the present invention provides a Chinese medicine composition comprising the following raw materials: lotus leaf, Sophora japonica flower, Hippophae rhamnoides, Imperata cylindrica root, Codonopsis pilosula, raw Astragalus membranaceus, and stir-fried chicken gizzard lining.

[0011] In some specific embodiments of the present invention, the traditional Chinese medicine composition comprises a Soxhlet alcohol extract of the drug substance.

[0012] In some specific embodiments of the present invention, the preparation method of the Soxhlet alcohol extract of the raw material drug is: crushing the raw material drug into powder, wrapping it with filter paper, mixing it with 15 times the volume of 90% ethanol, performing Soxhlet extraction, collecting the extract and performing vacuum low-temperature freeze-drying to obtain the Soxhlet alcohol extract of the raw material drug.

[0013] In some specific embodiments of the present invention, the heating level of the extraction stage of the Soxhlet extraction is 16, the extraction temperature is 70°C to 90°C, and the extraction time is 3h;

[0014] The heating level of the drying stage of the Soxhlet extraction is 10, the drying temperature is 60° C. to 80° C., and the drying time is 20 minutes.

[0015] In some specific embodiments of the present invention, the conditions for vacuum low-temperature freeze-drying are: vacuum degree 10Pa, temperature -60°C, and time 24h.

[0016] In some specific embodiments of the present invention, the Chinese medicine composition comprises, by weight, the following Soxhlet alcohol extracts of the raw materials:

[0017]

[0018] In a second aspect, the present invention also provides the use of the traditional Chinese medicine composition in the preparation of a drug for promoting pancreatic β cell proliferation and / or improving pancreatic β cell function.

[0019] In a third aspect, the present invention also provides the use of the traditional Chinese medicine composition in the preparation of a drug for preventing, improving and / or treating diabetes.

[0020] In a fourth aspect, the present invention also provides medicines, including the Chinese medicine composition.

[0021] In a fifth aspect, the present invention also provides a pharmaceutical preparation comprising the drug.

[0022] The present invention selects natural and non-toxic edible medicines, and simultaneously evaluates the effect of the edible-medicine combination on the proliferation of pancreatic β cells, so as to obtain an efficient edible-medicine combination that promotes the proliferation of pancreatic β cells. The results show that the combined use of 7 edible-medicine medicines, including lotus leaf, Sophora japonica flower, Hippophae rhamnoides, Imperata cylindrica root, Codonopsis pilosula, Raw Astragalus membranaceus, and stir-fried chicken gizzard lining, can synergistically promote the proliferation of pancreatic β cells and improve the function of pancreatic β cells, and can prevent, improve and / or treat diabetes. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0024] Figure 1 The survival rate results of Examples 1 to 3 are shown; wherein, Example vs. Control Group, *P<0.05, ***P<0.001;

[0025] Figure 2 The survival rate results of the examples and comparative examples are shown; among them, examples / comparative examples vs control group, *P<0.05, ***P<0.001; comparative examples vs example 1, ##P<0.01, ### P<0.001. DETAILED DESCRIPTION

[0026] The present invention discloses a Chinese herbal medicine extract composition that promotes the proliferation of pancreatic beta cells. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The methods and applications of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0027] The present invention aims to provide a Chinese medicinal and edible medicinal composition for promoting the proliferation of pancreatic β cells. The composition has the effect of synergistically promoting the proliferation of pancreatic β cells, can improve the function of pancreatic β cells, and is particularly suitable for diabetic patients.

[0028] A Chinese medicine composition for promoting pancreatic β cell proliferation specifically comprises:

[0029] 1) The traditional Chinese medicine composition comprises 7 kinds of edible and medicinal materials, namely, lotus leaf sorbxl extract, sophora japonica flower sorbxl extract, seabuckthorn sorbxl extract, Imperata cylindrica root sorbxl extract, Codonopsis pilosula sorbxl extract, raw astragalus radix sorbxl extract and stir-fried chicken gizzard lining sorbxl extract.

[0030] 2) The proportions of the Soxhlet alcohol extracts of the various Chinese medicines are as follows: 7-30 parts of lotus leaf Soxhlet alcohol extract, 2-9 parts of Soxhlet alcohol extract of Sophora japonica, 3-15 parts of Hippophae rhamnoides, 2-9 parts of Imperata cylindrica root Soxhlet alcohol extract, 4-20 parts of Codonopsis pilosula Soxhlet alcohol extract, 7-30 parts of raw Astragalus membranaceus Soxhlet alcohol extract, and 3-15 parts of stir-fried chicken gizzard lining. Among them, Astragalus membranaceus and Codonopsis pilosula are the main drugs, lotus leaf, stir-fried chicken gizzard lining, and Hippophae rhamnoides are the ministerial drugs, Imperata cylindrica root is the adjuvant drug, and Sophora japonica is the guiding drug.

[0031] 3) The preparation method of the Soxhlet alcohol extract is as follows: crush the medicinal material to form powder, weigh 10g of the powder and put it on filter paper to wrap it into a medicine bag, put the medicine bag into the bottom of the cylindrical glass sample tube of the Soxhlet extractor, then pour 15 times the volume of 90% ethanol into the solvent cup and fix it at the lower end interface of the Soxhlet extractor, set the heating level of the extraction stage to 16 and the extraction time to 3h; set the heating level of the drying stage to 10 and the drying time to 20min, and perform Soxhlet extraction. After the extraction is completed, the extract is vacuum-freeze-dried to finally obtain the Soxhlet alcohol extract.

[0032] 4) The Chinese medicine composition has a stronger effect on promoting the proliferation of pancreatic β cells than the pharmaceutical compositions lacking the Soxhlet ethanol extract of Codonopsis pilosula, lacking the Soxhlet ethanol extract of Hippophae rhamnoides, lacking the Soxhlet ethanol extract of Sophora japonica, and lacking the Soxhlet ethanol extract of Imperata cylindrica.

[0033] 5) The present invention uses the Chinese herbal medicine extract composition as both medicine and food mainly for improving pancreatic β cells in patients with diabetes.

[0034] The raw materials and reagents used in the edible Chinese medicinal extract composition for promoting the proliferation of pancreatic beta cells provided by the present invention can all be purchased from the market.

[0035] The present invention will be further described below in conjunction with embodiments:

[0036] Preparation of Chinese medicine Soxhlet alcohol extract:

[0037] The specific process is:

[0038] 1. Preparation of lotus leaf Soxhlet alcohol extract: crush lotus leaf medicinal material to form lotus leaf powder, weigh 10g lotus leaf powder and put it on filter paper to wrap it into a medicine bag, put the medicine bag into the bottom of the cylindrical glass sample tube of the Soxhlet extractor, then pour 15 times the volume of 90% ethanol into the solvent cup and fix it to the interface at the lower end of the Soxhlet extractor, extract according to the standard Soxhlet extraction method, set the heating level of the extraction stage to 16, the extraction temperature to 70℃~90℃, and the extraction time to 3h; set the heating level of the drying stage to 10, the drying temperature to 60℃~80℃, and the drying time to 20min, and perform Soxhlet extraction. After the extraction is completed, the extract is vacuum-freeze-dried (the conditions of vacuum-freeze-drying are: vacuum degree 10Pa, temperature -60℃, time 24h), and finally obtain the lotus leaf Soxhlet alcohol extract.

[0039] 2. The preparation methods of the Sophora japonica flower Soxhlet extract, Hippophae rhamnoides flower Soxhlet extract, Imperata cylindrica root Soxhlet extract, Codonopsis pilosula Soxhlet extract, Raw Astragalus membranaceus flower Soxhlet extract and Stir-fried Gallus gallus tuber are the same as those of the lotus leaf Soxhlet extract.

[0040] Embodiment 1:

[0041] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0042] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides root Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 15 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0043] Embodiment 2:

[0044] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0045] Take 7 parts of lotus leaf Soxhlet ethanol extract, 2 parts of Sophora japonica flower Soxhlet ethanol extract, 3 parts of Hippophae rhamnoides root Soxhlet ethanol extract, 2 parts of Imperata cylindrica root Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 15 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0046] Embodiment 3:

[0047] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0048] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides root Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 4 parts of Codonopsis pilosula Soxhlet ethanol extract, 7 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 3 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0049] Comparative Example 1 (lack of Codonopsis pilosula):

[0050] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0051] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus and 15 parts of stir-fried Gizzard nut of Gallus gallus domesticus, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0052] Comparative Example 2 (lacking seabuckthorn):

[0053] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0054] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 15 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0055] Comparative Example 3 (lack of Sophora japonica):

[0056] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0057] Take 30 parts of lotus leaf Soxhlet ethanol extract, 15 parts of seabuckthorn Soxhlet ethanol extract, 9 parts of Imperata root Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 15 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0058] Comparative Example 4 (lack of Imperata rhizome):

[0059] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0060] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract, 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 15 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0061] Comparative Example 5 (lack of fried chicken gizzard lining):

[0062] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0063] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 20 parts of Codonopsis pilosula Soxhlet ethanol extract and 30 parts of raw Astragalus membranaceus Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0064] Comparative Example 6 (replacing the Codonopsis pilosula in Example 1):

[0065] The following Soxhlet alcohol extracts were all prepared according to the methods described in the preparation examples.

[0066] Take 30 parts of lotus leaf Soxhlet ethanol extract, 9 parts of Sophora japonica flower Soxhlet ethanol extract, 15 parts of Hippophae rhamnoides root Soxhlet ethanol extract, 9 parts of Imperata cylindrica root Soxhlet ethanol extract, 20 parts of Polygonum multiflorum Soxhlet ethanol extract, 7 parts of raw Astragalus membranaceus Soxhlet ethanol extract and 3 parts of stir-fried Gallus gallus endometrium Soxhlet ethanol extract, dilute with DMSO to form a 50 mg / mL sample, and then dilute with complete culture medium to form a 50 μg / mL sample.

[0067] Effect example

[0068] The Cell Counting Kit-8 reagent was used to evaluate the effects of each sample in the embodiment and the comparative example on the proliferation of rat β insulinoma cells (RIN-m5F cells). The specific process is as follows:

[0069] RIN-m5F cells were cultured in 1640 medium containing 10% FBS, 100 U / mL penicillin, and 100 μg / mL streptomycin at 37°C in an incubator containing 5% CO2. When the cells grew to the logarithmic growth phase, that is, when the cell density was about 80%, the cells were passaged. RIN-m5F cells in the logarithmic growth phase were taken and 1×10 5 The density of cells / mL was inoculated in a 96-well plate, and 100 μL was inoculated in each well. After 24 hours of culture, the following experimental groups were used for operation: a control group (complete medium containing 0.2% DMSO), 10 drug administration groups (containing Examples 1 to 3 and Comparative Examples 1 to 6, respectively), 48 hours later, the liquid was discarded, 100 μL of CCK8 solution was added to each well, and the absorbance was measured at 450 nm with an ELISA reader after 2 hours. The absorbance value of 100 μL of CCK8 solution in the well plate was used as a blank group, and the formula: survival rate (%) = [(OD value of drug administration group-OD value of blank group)-(OD value of control group-OD value of blank group)] / (OD value of control group-OD value of blank group)*100% was used to calculate the cell survival rate, and SPSS was used for experimental statistics.

[0070] Effects of different proportions of the examples on the proliferation of RIN-m5F cells:

[0071] 1. Using the above formula, the effects of Examples 1 to 3 on the proliferation of RIN-m5F cells at a concentration of 50 μg / mL were calculated respectively.

[0072] Based on the drug combination, we designed three embodiments with different ratios. The results of cell survival rates after the three embodiments acted on RIN-m5F cells are shown in Table 1.

[0073] from Figure 1 It can be seen that Examples 1 to 3 significantly promoted the proliferation of RIN-m5F cells compared with the control group (*P<0.05, ***P<0.001), indicating that the different proportions of the combination promoted the proliferation of RIN-m5F cells. In addition, Example 1 had the strongest effect on promoting the proliferation of RIN-m5F cells, and the cell survival rate reached 148.45%.

[0074] Table 1 Survival rate results of Examples 1 to 3

[0075]

[0076] 2. The above formula was used to calculate the effects of Comparative Examples 1 to 6 on the proliferation of RIN-m5F cells at a concentration of 50 μg / mL. The results are shown in Tables 2 and Figure 2 shown.

[0077] Based on the proportions of Example 1, we designed drug-deficient groups or drug-replacement groups as comparison examples, including comparison example 1 (lacking Codonopsis pilosula), comparison example 2 (lacking Hippophae rhamnoides), comparison example 3 (lacking Sophora japonica), comparison example 4 (lacking Imperata cylindrica), comparison example 5 (lacking fried chicken gizzard lining), and a drug-replacement group, comparison example 6 (replacing Codonopsis pilosula with Polygonum multiflorum). The results of the cell survival rates of the comparison examples after acting on RIN-m5F cells are shown in Table 2.

[0078] from Figure 2 It can be seen that compared with the control group, only Example 1 and Comparative Examples 3, 4 and 6 significantly promoted the proliferation of RIN-m5F cells (*P<0.05, ***P<0.001). However, compared with Example 1, the survival rates of Comparative Examples 1 to 6 on RIN-m5F cells were significantly weaker than those of Example 1 (##P<0.01, ### P<0.001), indicating that Example 1 significantly promoted the proliferation of RIN-m5F cells better than Comparative Example 1 (lack of Codonopsis pilosula), Comparative Example 2 (lack of Hippophae rhamnoides), Comparative Example 3 (lack of Sophora japonica), Comparative Example 4 (lack of Imperata cylindrica), Comparative Example 5 (lack of stir-fried chicken gizzard lining), and the dressing change group, Comparative Example 6 (replacing Codonopsis pilosula with Rhizoma Polygoni Multiflori).

[0079] Table 2 Survival rate results of comparative examples 1 to 6

[0080]

[0081]

[0082] 3. The above results confirm that the combination of the embodiments (comprising lotus leaf Soxhlet ethanol extract, Soxhlet ethanol extract of Sophora japonica flower, Soxhlet ethanol extract of Hippophae rhamnoides, Soxhlet ethanol extract of Imperata cylindrica root, Soxhlet ethanol extract of Codonopsis pilosula, Soxhlet ethanol extract of raw Astragalus membranaceus, and Soxhlet ethanol extract of stir-fried chicken's gizzard lining) at different proportions all has the effect of promoting RIN-m5F cell proliferation, and Embodiment 1 is significantly better than the various drug-deficient comparison groups and drug-replacement comparison groups at the same proportion and the same concentration, including Comparison Example 1 lacking Codonopsis pilosula, Comparison Example 2 lacking Hippophae rhamnoides, Comparison Example 3 lacking Sophora japonica flower, Comparison Example 4 lacking Imperata cylindrica root, Comparison Example 5 lacking stir-fried chicken's gizzard lining, and Comparison Example 6 in which Codonopsis pilosula is replaced with Polygonum multiflorum, indicating that the whole prescription combination has a stronger effect on promoting RIN-m5F cell proliferation than the drug-deficient group, and is more suitable for improving the condition of pancreatic islet cells in diabetic patients.

[0083] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Chinese medicine composition, It is characterized in that The raw materials include: lotus leaves, Sophora japonica flowers, sea buckthorn, Imperata cylindrica roots, Codonopsis pilosula, raw Astragalus membranaceus, and fried chicken gizzard lining.

2. The Chinese medicine composition according to claim 1, It is characterized in that The invention comprises a Soxhlet alcohol extract of the raw material drug.

3. The Chinese medicine composition as claimed in claim 2, It is characterized in that The preparation method of the Soxhlet alcohol extract of the raw material drug is as follows: the raw material drug is crushed into powder, wrapped with filter paper, mixed with 15 times the volume of 90% ethanol, subjected to Soxhlet extraction, and the extract is collected for vacuum low-temperature freeze-drying to obtain the Soxhlet alcohol extract of the raw material drug.

4. The Chinese medicine composition according to claim 2 or 3, It is characterized in that The heating level of the extraction stage of the Soxhlet extraction is 16, the extraction temperature is 70°C to 90°C, and the extraction time is 3h; The heating level of the drying stage of the Soxhlet extraction is 10, the drying temperature is 60° C. to 80° C., and the drying time is 20 minutes.

5. The Chinese medicine composition according to any one of claims 2 to 4, It is characterized in that The vacuum low-temperature freeze-drying conditions are: vacuum degree 10Pa, temperature -60°C, and time 24h.

6. The Chinese medicine composition according to any one of claims 2 to 5, It is characterized in that In parts by weight, the Soxhlet alcohol extract of the following raw materials is included:

7. Use of the Chinese medicine composition according to any one of claims 1 to 6 in the preparation of a medicament for promoting pancreatic β cell proliferation and / or improving pancreatic β cell function.

8. Use of the Chinese medicine composition according to any one of claims 1 to 6 in the preparation of a medicament for preventing, improving and / or treating diabetes.

9. Medication, It is characterized in that Comprising the Chinese medicine composition as described in any one of claims 1 to 6.

10. Pharmaceutical preparations, It is characterized in that Including the drug as claimed in claim 9.

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