Preparation method of fish oil-loaded calcium alginate micro-capsule with nanoscale particle size

Abstract

The invention relates to the technical field of microcapsule preparation, in particular to a nano-scale particle size fish oil-loaded calcium alginate microcapsule preparation method, which comprises: S1, carrying out primary cleaning on collected fish oil, removing floating dirt by using a skimmer, carrying out step-by-step filtration treatment by using filter screens with different mesh numbers, firstly carrying out primary filtration by using a 90-mesh filter screen, and then carrying out secondary filtration by using a 90-mesh filter screen; filtering the fish oil again by using a 150-mesh filter screen and a 250-mesh filter screen in sequence; s2, removing excessive moisture from the filtered fish oil by adopting a vacuum drying mode, and enabling the viscosity to be 10 <-4 >-10 <-7 > Pa.s when the temperature is 23-28 DEG C; s3, adding 0.02%-0.05% of an antioxidant into the fish oil in the S2, and stirring the fish oil with a magnetic stirrer until the fish oil is completely dissolved to obtain antioxidant fish oil; the complexity of converting the emulsion into different equipment in different steps is reduced, the production cost and the material loss are reduced, the equipment is easy to operate and control, and the working efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of microcapsule preparation, in particular to a method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules. Background Art

[0002] In the field of food processing and nutritional supplements, fish oil, as a high-quality raw material rich in polyunsaturated fatty acids (PUFA), is widely used in various health products and health foods. With the development of nanotechnology, nanoscale microcapsules have gradually become a research hotspot due to their small particle size, large specific surface area, and easy absorption by the human body.

[0003] At present, in the existing preparation methods, such as the patent with authorization announcement number CN105533691B, the invention relates to a fish oil / algae oil microencapsulated product rich in polyunsaturated fatty acids (PUFA) necessary for the human body and its production process. The microcapsules are prepared by a rapid membrane emulsification process using biodegradable sodium alginate and chitosan as wall materials through a complex coacervation method.

[0004] However, during the use of this process, it was found that different equipment was needed to prepare the microcapsules in different steps, which increased the complexity of equipment use and reduced work efficiency. In addition, the preparation of nano-scale microcapsules through SPG membranes increased production costs and increased the complexity of maintenance. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a method for preparing nano-sized fish oil-loaded calcium alginate microcapsules, which reduces the complexity of converting emulsions into different equipment in different steps, reduces production costs and material losses, makes the equipment easy to operate and control, and improves work efficiency.

[0006] The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules of the present invention comprises the following steps: S1, preliminarily washing the collected fish oil and removing floating dirt with a skimmer, and then filtering the fish oil step by step with filter screens of different mesh sizes, first filtering the fish oil once with a 90-mesh filter screen, and then filtering the fish oil again with filter screens of 150 mesh and 250 mesh in sequence;

[0007] S2, removing excess water from the filtered fish oil by vacuum drying, so that the viscosity of the fish oil is 10-4 to 10-7 Pa·s at a temperature of 23-28°C;

[0008] S3, adding 0.02%-0.05% antioxidant to the fish oil in S2, stirring with a magnetic stirrer until completely dissolved, to obtain antioxidant fish oil;

[0009] S4, dissolving sodium alginate in deionized water and heating to 55-60° C. to make the concentration of sodium alginate 1.0%-2.0%, slowly adding emulsifier and stabilizer during heating, and stirring well to obtain sodium alginate aqueous solution;

[0010] S5, first put the sodium alginate aqueous solution into the homogenizing device, then slowly add the antioxidant fish oil into the homogenizing device to stir and mix to form a stable emulsion, and the temperature in the homogenizing device is controlled at 23-28°C;

[0011] S6. In S5, the emulsion is sheared and ultrasonically vibrated by a homogenizing device, and the emulsion is made to reach the nanometer level by utilizing the cavitation effect and shear mechanical effect of the ultrasound;

[0012] S7, slowly adding the calcium chloride solution to the nanoemulsion in S6, stirring while adding, so that the sodium alginate and the calcium chloride fully react to form a calcium alginate shell layer and embed the fish oil, to obtain a nanoscale fish oil-loaded calcium alginate microcapsule suspension;

[0013] S8, centrifuging the nanoscale fish oil-loaded calcium alginate microcapsule suspension to remove excess unreacted wall material, and then drying it;

[0014] S9. The dried microcapsules are sieved to obtain nano-sized calcium alginate microcapsules loaded with fish oil that meet the requirements; the sodium alginate aqueous solution and the antioxidant fish oil are stirred, mixed and sheared and emulsified by using a homogenizing device to improve the convenience of preparing nano-sized calcium alginate microcapsules loaded with fish oil, reduce the complexity of converting the emulsion to different equipment in different steps, reduce production costs and material losses, and use homogenizing equipment to complete different processing steps, which is easy to operate and control and improves work efficiency.

[0015] Preferably, in S5, the sodium alginate aqueous solution is placed in a homogenizing device and preheated to 23-28° C., and the antioxidant fish oil is kept at 23-28° C. and slowly added to the homogenizing device; thereby increasing the temperature consistency between the sodium alginate aqueous solution and the antioxidant fish oil and improving the stability of the emulsion formation.

[0016] Preferably, the homogenizing equipment comprises a supporting device, a circulating device, a driving device, a tank body, a first three-way pipe, two groups of first valves, an electric heater, an ultrasonic transducer, a frame, multiple groups of first blades and multiple groups of second blades, the first three-way pipe is connected and arranged on the outer wall of the tank body, the two groups of first valves are connected and arranged on two groups of input ends of the first three-way pipe respectively, the electric heater and the ultrasonic transducer are respectively installed on the inner wall of the tank body, the frame is rotatably installed on the inner wall of the tank body, the multiple groups of first blades are all installed on the outer wall of the frame, the multiple groups of second blades are all installed on the supporting device, and the support The device is used to drive the multiple groups of second blades to vibrate up and down, and the supporting device is used to transport the calcium chloride solution into the tank body, the multiple groups of second blades and the multiple groups of first blades are arranged in a staggered manner, the circulation device is arranged on the tank body, the circulation device is used to circulate the material in the tank body, the driving device is arranged on the tank body, and the driving device is used to drive the frame to rotate; the sodium alginate aqueous solution is put into the tank body through the first group of input ends of the first three-way pipe, and then the sodium alginate aqueous solution in the tank body is heated by the electric heater, and then the antioxidant fish oil is transported into the tank body through the second group of input ends of the first three-way pipe. The plurality of first blades are sent to the inside of the tank body, and the frame body is driven to rotate by the driving device. After the frame body rotates, the plurality of first blades are driven to move circumferentially. At the same time, the frame body drives the supporting device to rotate, so that the supporting device drives the plurality of second blades to move circumferentially, so that the plurality of first blades and the plurality of second blades mix and stir the materials in the tank body into an emulsion. At the same time, the circulation device extracts the emulsion at the bottom of the tank body and refluxes it upward into the tank body, thereby improving the uniform preparation effect of the circulation of the emulsion. After the emulsion is stirred, the plurality of second blades are driven to vibrate up and down by the supporting device, so that the plurality of second blades cooperate with the first blades on both sides to shear the emulsion during the up and down movement of the plurality of second blades. At the same time, the ultrasonic transducer is turned on to improve the crushing effect of the emulsion. After the emulsion is prepared, the calcium chloride solution is added into the tank body through the supporting device, and the materials are continuously stirred by the first blade and the second blade, so that the sodium alginate and the calcium chloride are fully reacted to form a calcium alginate shell layer and embed the fish oil, so as to obtain a nano-scale fish oil-carrying calcium alginate microcapsule suspension, thereby improving the multi-use effect of the equipment, reducing the complexity of equipment replacement during the preparation process, and improving the preparation efficiency and convenience.

[0017] Preferably, the supporting device comprises a conveying device, a spline sleeve, a hollow shaft, a top plate, multiple groups of guide columns, multiple groups of springs and a vibration motor. The spline sleeve is mounted on the outer wall of the frame body, the lower part of the hollow shaft is slidably mounted on the spline sleeve by splines, the upper part of the hollow shaft is rotatably mounted on the top plate, the top end of the hollow shaft is connected with the conveying device, the conveying device is used to transport the calcium chloride solution to the inside of the hollow shaft, the outer wall of the hollow shaft is provided with a discharge port, the discharge port is communicated with the tank body, multiple groups of guide columns are all mounted on the top of the tank body, the top plate is slidably mounted on the multiple groups of guide columns, multiple groups of springs are respectively mounted on the outer walls of the multiple groups of guide columns, the vibration motor is mounted on the outer wall of the top plate, and multiple groups of second knives are mounted on the outer wall of the top plate. The blades are all installed on the outer wall of the hollow shaft; the frame rotates and drives the spline sleeve to rotate, and the spline sleeve rotates and drives the hollow shaft to rotate, so that the hollow shaft drives the second blade to move circumferentially, and then the multiple groups of second blades and the multiple groups of first blades move circumferentially synchronously, and the vibration motor is turned on to drive the top plate to vibrate up and down through the multiple groups of guide columns and the multiple groups of springs, so that the top plate drives the hollow shaft to vibrate up and down, and the hollow shaft drives the multiple groups of second blades to vibrate up and down to shear and crush the emulsion in the tank body, and the calcium chloride solution is transported to the inside of the hollow shaft through the conveying device, so that the hollow shaft adds the calcium chloride solution into the tank body through the discharge port to mix with the emulsion, thereby improving the convenience of microcapsule preparation.

[0018] Preferably, the driving device includes a gear ring, a transfer case, a driving motor, a gear, a clutch, a disc, a connecting rod, a cylinder and a one-way valve, the gear ring is mounted on the outer wall of the frame, the transfer case is mounted on the inner wall of the tank, the driving motor is mounted on the outer wall of the transfer case, the output end of the driving motor is connected to the transfer case, the gear is mounted on the first output end of the transfer case, the gear is meshed with the gear ring, the clutch is connected to the second output end of the transfer case, the disc is mounted on the output end of the clutch, one end of the connecting rod is rotatably connected to the eccentric position of the outer wall of the disc, and the other end of the connecting rod is rotatably connected to the piston, The piston is slidingly arranged inside the cylinder, and the cylinder is installed on the outer side wall of the tank body. Two groups of one-way valves are respectively connected and arranged on the outer side wall of the cylinder, and one group of one-way valves is connected with the conveying device. The transfer case is powered by a driving motor, so that the transfer case drives the gear and the clutch respectively. After the gear rotates, it drives the frame to rotate by meshing with the gear ring, and the clutch drives the disc to rotate. The disc drives the piston to reciprocate in the cylinder through the connecting rod, so that the cylinder is pressurized in the conveying device through the cooperation of the two groups of one-way valves, thereby improving the convenience of conveying the calcium chloride solution by pressure.

[0019] Preferably, the conveying device includes a connecting device, an air bag, a storage tank, a second valve and a third valve, the air bag is connected to one group of one-way valves, a feed valve is arranged on the outer wall of the storage tank, the storage tank is connected to the air bag through the second valve, the third valve is arranged on the storage tank, the bottom end of the third valve extends into the bottom of the storage tank through a pipeline, and the output end of the third valve is connected to the hollow shaft through the connecting device; when the materials in the tank body are mixed and prepared, the air is conveyed to the air bag through the one-way valve for compressed storage, and the calcium chloride solution is placed in the storage tank for storage, and when the calcium chloride solution needs to be added to the emulsion in the tank body, the third valve and the second valve are opened, so that the compressed air in the air bag enters the storage tank, and the air compresses the calcium chloride solution in the storage tank and conveys it to the hollow shaft, thereby improving the convenience of adding the calcium chloride solution into the tank body.

[0020] Preferably, the circulation device includes a transmission device, a pump body, a second three-way pipe, two groups of fourth valves and a delivery pipe, the pump body is installed on the outer wall of the tank body, the power input end of the pump body is connected to the gear ring through the transmission device, the second three-way pipe is connected and arranged on the output end of the pump body, one group of output ends of the second three-way pipe is connected with the tank body, the delivery pipe is connected and arranged on the input end of the pump body, the input end of the delivery pipe is connected and arranged at the lower part of the tank body, and the two groups of fourth valves are connected and arranged on the two groups of output ends of the second three-way pipe respectively; after the gear ring rotates, the pump body is driven by the transmission device, so that the pump body extracts the material at the bottom of the tank body through the delivery pipe, and the extracted material flows back into the tank body through the first output end of the second three-way pipe, thereby improving the effect of the up and down circulation of the material in the tank body, improving the uniformity of material preparation, and when the material in the tank body needs to be discharged, the material is discharged through the second output end of the second three-way pipe, thereby improving the convenience of equipment use.

[0021] Preferably, the connecting device includes a hose and a delivery box, the hose input end is connected to the third valve output end, the hose output end is connected to the delivery box, the delivery box is installed on the top of the top plate, and the upper part of the hollow shaft rotates to extend into the interior of the delivery box, the upper part of the hollow shaft is provided with an inlet, and the inlet is connected to the delivery box; the third valve transports the calcium chloride solution in the storage tank to the delivery box through the hose, and the calcium chloride solution is transported to the hollow shaft through the delivery box, thereby improving the convenience of the hollow shaft adding the calcium chloride solution into the tank body.

[0022] Preferably, the transmission device includes two sets of pulleys and belts, the first set of pulleys is installed on the outer wall of the gear ring, the second set of pulleys is installed on the power input end of the pump body, and the belt is sleeved on the outer walls of the two sets of pulleys; after the gear ring rotates, the pump body is driven by the two sets of pulleys and belts, thereby improving the convenience of the pump body in circulating or discharging the material in the tank body.

[0023] Preferably, it also includes a plurality of groups of nozzles, which are all connected and arranged at the discharge outlet of the hollow shaft; the calcium chloride solution discharged from the hollow shaft is transported through the plurality of groups of nozzles to improve the uniformity of the discharge of the calcium chloride solution.

[0024] Compared with the prior art, the present invention has the following beneficial effects: by adopting a homogenizing device to stir, mix and shear emulsify the sodium alginate aqueous solution and the antioxidant fish oil, the convenience of preparing nano-sized calcium alginate microcapsules loaded with fish oil is improved, the complexity of converting the emulsions to different equipment in different steps is reduced, the production cost and material loss are reduced, and by using the homogenizing device to complete different processing steps, it is easy to operate and control, thereby improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the axonometric structure of the present invention;

[0026] Figure 2 is an axonometric partial structural diagram of the connection between the first three-way pipe and the first valve, etc.;

[0027] Figure 3 It is a schematic diagram of the partial axonometric structure of the connection between the frame body and the first blade, etc.;

[0028] Figure 4 It is a schematic diagram of the partial structure of the axonometric view of the connection between the clutch and the disc, etc.;

[0029] Figure 5 It is an axonometric structural diagram of the connection between the air bag and the second valve, etc.;

[0030] Figure 6 It is a schematic diagram of the axonometric partial structure of the connection between the pump body and the delivery pipe, etc.;

[0031] Figure 7 It is a schematic diagram of the axonometric structure of the connection between the cylinder and the one-way valve, etc.;

[0032] Figure 8 It is a schematic diagram of the axonometric structure of the connection between the top plate and the vibration motor, etc.;

[0033] Fig. 9 It is a schematic diagram of the axonometric local structure of the connection between the tank body and the electric heater, etc.;

[0034] Fig.10 It is a schematic diagram of the axonometric partial structure of the connection between the hollow shaft and the conveying box, etc.;

[0035] Fig.11 It is a schematic diagram of the partial isometric structure of the connection between the third valve and the hose, etc.;

[0036] Fig.12 It is an axonometric diagram of the partial structure of the connection between the pulley and the gear ring.

[0037] The reference numerals in the accompanying drawings are: 101, tank body; 102, first three-way pipe; 103, first valve; 104, electric heater; 105, ultrasonic transducer; 106, frame; 107, first blade; 108, second blade; 201, spline sleeve; 202, hollow shaft; 203, top plate; 204, guide column; 205, spring; 206, vibration motor; 301, gear ring; 302, transfer case; 303, drive motor; 304, gear ring; 305, gear ring; 306, gear ring; 307, gear ring; 308, gear ring; 309, gear ring; 310, gear ring; 311, gear ring; 312, gear ring; 313, gear ring; 314, gear ring; 315, gear ring; 316, gear ring; 317, gear ring; 318, gear ring; 319, gear ring; 320, gear ring; 321, gear ring; 322, gear ring; 323, gear ring; 324, gear ring; 325, gear ring; 326, gear ring; 327, gear ring; 328, gear ring; 329, gear ring; 330, gear ring; 331, gear ring; 332, gear ring; 333, gear ring; 334, gear ring; 335, gear ring; 336, gear ring; 337, gear ring; 338, gear ring; 339, gear ring; 340, gear ring; 341, gear ring; 342, gear ring; 343, gear ring; 344, gear ring; 345, gear ring; 346, gear ring; 347, gear ring; 348, gear ring 04, gear; 305, clutch; 306, disc; 307, connecting rod; 308, cylinder; 309, one-way valve; 401, air bag; 402, storage tank; 403, second valve; 404, third valve; 501, pump body; 502, second three-way pipe; 503, fourth valve; 504, delivery pipe; 601, hose; 602, delivery box; 701, pulley; 702, belt; 801, nozzle. DETAILED DESCRIPTION

[0038] In order to facilitate understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0039] Example 1

[0040] The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules of the present invention comprises the following steps: S1, preliminarily washing the collected fish oil and removing floating dirt with a skimmer, and then filtering the fish oil step by step with filter screens of different mesh sizes, first filtering the fish oil once with a 90-mesh filter screen, and then filtering the fish oil again with filter screens of 150 mesh and 250 mesh in sequence;

[0041] S2, removing excess water from the filtered fish oil by vacuum drying, so that the viscosity of the fish oil is 10-4 to 10-7 Pa·s at a temperature of 23-28°C;

[0042] S3, adding 0.02%-0.05% antioxidant to the fish oil in S2, stirring with a magnetic stirrer until completely dissolved, to obtain antioxidant fish oil;

[0043] S4, dissolving sodium alginate in deionized water and heating to 55-60° C. to make the concentration of sodium alginate 1.0%-2.0%, slowly adding emulsifier and stabilizer during heating, and stirring well to obtain sodium alginate aqueous solution;

[0044] S5, first put the sodium alginate aqueous solution into the homogenizing device, then slowly add the antioxidant fish oil into the homogenizing device to stir and mix to form a stable emulsion, and the temperature in the homogenizing device is controlled at 23-28°C;

[0045] S6. In S5, the emulsion is sheared and ultrasonically vibrated by a homogenizing device, and the emulsion is made to reach the nanometer level by utilizing the cavitation effect and shear mechanical effect of the ultrasound;

[0046] S7, slowly adding the calcium chloride solution to the nanoemulsion in S6, stirring while adding, so that the sodium alginate and the calcium chloride fully react to form a calcium alginate shell layer and embed the fish oil, to obtain a nanoscale fish oil-loaded calcium alginate microcapsule suspension;

[0047] S8, centrifuging the nanoscale fish oil-loaded calcium alginate microcapsule suspension to remove excess unreacted wall material, and then drying it;

[0048] S9, screening the dried microcapsules to obtain fish oil-loaded calcium alginate microcapsule products with nano-scale particle size that meet the requirements;

[0049] In S5, the sodium alginate aqueous solution is placed in a homogenizing device and preheated to 23-28° C., and the antioxidant fish oil is maintained at a temperature of 23-28° C. and slowly added to the homogenizing device;

[0050] In this embodiment, a homogenizing device is used to stir, mix and shear emulsify the sodium alginate aqueous solution and the antioxidant fish oil, thereby improving the convenience of preparing nano-sized calcium alginate microcapsules loaded with fish oil, reducing the complexity of converting the emulsions to different equipment in different steps, reducing production costs and material losses, and using a homogenizing device to complete different processing steps makes it easy to operate and control, thereby improving work efficiency.

[0051] Example 2

[0052] On the basis of Example 1, a method for preparing nano-particle size fish oil-loaded calcium alginate microcapsules of the present invention is provided, wherein the homogenizing device comprises a supporting device, a circulating device, a driving device, a tank body 101, a first three-way pipe 102, two groups of first valves 103, an electric heater 104, an ultrasonic transducer 105, a frame 106, a plurality of groups of first blades 107 and a plurality of groups of second blades 108, the first three-way pipe 102 is connected and arranged on the outer wall of the tank body 101, the two groups of first valves 103 are respectively connected and arranged on the two groups of input ends of the first three-way pipe 102, the electric heater 104 and the ultrasonic transducer 105 are respectively installed on the tank body. 101 inner wall, the frame 106 is rotatably mounted on the inner wall of the tank body 101, multiple groups of first blades 107 are mounted on the outer wall of the frame 106, multiple groups of second blades 108 are mounted on the supporting device, the supporting device is used to drive the multiple groups of second blades 108 to vibrate up and down, and the supporting device is used to transport the calcium chloride solution into the tank body 101, the multiple groups of second blades 108 and the multiple groups of first blades 107 are staggered, the circulation device is arranged on the tank body 101, the circulation device is used to circulate the material in the tank body 101, and the driving device is arranged on the tank body 101, and the driving device is used to drive the frame 106 to rotate;

[0053] The supporting device includes a conveying device, a spline sleeve 201, a hollow shaft 202, a top plate 203, multiple groups of guide columns 204, multiple groups of springs 205 and a vibration motor 206. The spline sleeve 201 is installed on the outer wall of the frame 106, the lower part of the hollow shaft 202 is slidably installed on the spline sleeve 201 through a spline, the upper part of the hollow shaft 202 is rotatably installed on the top plate 203, the top of the hollow shaft 202 is connected with the conveying device, the conveying device is used to transport the calcium chloride solution to the inside of the hollow shaft 202, the outer wall of the hollow shaft 202 is provided with a discharge port, and the discharge port is communicated with the tank body 101, the multiple groups of guide columns 204 are all installed on the top of the tank body 101, the top plate 203 is slidably installed on the multiple groups of guide columns 204, the multiple groups of springs 205 are respectively sleeved on the outer walls of the multiple groups of guide columns 204, the vibration motor 206 is installed on the outer wall of the top plate 203, and the multiple groups of second blades 108 are all installed on the outer wall of the hollow shaft 202;

[0054] The driving device includes a gear ring 301, a transfer case 302, a driving motor 303, a gear 304, a clutch 305, a disc 306, a connecting rod 307, a cylinder 308 and a one-way valve 309. The gear ring 301 is mounted on the outer wall of the frame 106, the transfer case 302 is mounted on the inner wall of the tank 101, the driving motor 303 is mounted on the outer wall of the transfer case 302, the output end of the driving motor 303 is connected to the transfer case 302, the gear 304 is mounted on the first output end of the transfer case 302, and the gear 304 is mounted on the first output end of the transfer case 302. 4 is meshed with the gear ring 301, the clutch 305 is connected to the second output end of the transfer case 302, the disc 306 is installed on the output end of the clutch 305, one end of the connecting rod 307 is rotatably connected to the eccentric position of the outer wall of the disc 306, and the other end of the connecting rod 307 is rotatably connected to the piston, and the piston is slidably arranged inside the cylinder 308, and the cylinder 308 is installed on the outer wall of the tank body 101. Two sets of one-way valves 309 are respectively connected and arranged on the outer wall of the cylinder 308, and one set of one-way valves 309 is connected to the conveying device;

[0055] The conveying device includes a connecting device, an air bag 401, a storage tank 402, a second valve 403 and a third valve 404. The air bag 401 is connected to one of the one-way valves 309. A feed valve is arranged on the outer wall of the storage tank 402. The storage tank 402 is connected to the air bag 401 through the second valve 403. The third valve 404 is arranged on the storage tank 402. The bottom end of the third valve 404 extends into the bottom of the storage tank 402 through a pipeline. The output end of the third valve 404 is connected to the hollow shaft 202 through the connecting device.

[0056] The circulation device includes a transmission device, a pump body 501, a second three-way pipe 502, two groups of fourth valves 503 and a delivery pipe 504. The pump body 501 is installed on the outer wall of the tank body 101. The power input end of the pump body 501 is connected to the gear ring 301 through the transmission device. The second three-way pipe 502 is connected to the output end of the pump body 501. One group of output ends of the second three-way pipe 502 is connected to the tank body 101. The delivery pipe 504 is connected to the input end of the pump body 501. The input end of the delivery pipe 504 is connected to the lower part of the tank body 101. The two groups of fourth valves 503 are respectively connected to the two groups of output ends of the second three-way pipe 502.

[0057] The connecting device includes a hose 601 and a delivery box 602. The input end of the hose 601 is connected to the output end of the third valve 404, and the output end of the hose 601 is connected to the delivery box 602. The delivery box 602 is installed on the top of the top plate 203, and the upper part of the hollow shaft 202 rotates and extends into the interior of the delivery box 602. The upper part of the hollow shaft 202 is provided with an inlet, which is connected to the inside of the delivery box 602.

[0058] The transmission device includes two sets of pulleys 701 and belts 702. The first set of pulleys 701 is installed on the outer wall of the gear ring 301, and the second set of pulleys 701 is installed on the power input end of the pump body 501. The belts 702 are sleeved on the outer walls of the two sets of pulleys 701.

[0059] It also includes a plurality of nozzle groups 801, and the plurality of nozzle groups 801 are connected and arranged at the discharge outlet of the hollow shaft 202;

[0060] In this embodiment, the temperature consistency effect of the sodium alginate aqueous solution and the antioxidant fish oil is improved, and the stability of the emulsion formation is improved. The sodium alginate aqueous solution is placed into the tank body 101 through the first group of input ends of the first three-way pipe 102, and then the sodium alginate aqueous solution in the tank body 101 is heated by the electric heater 104. Then, the antioxidant fish oil is transported to the tank body 101 through the second group of input ends of the first three-way pipe 102, and the frame 106 is driven to rotate by the driving device. After the frame 106 rotates, it drives the multiple groups of first blades 107 to move circumferentially. At the same time, the frame 106 drives the supporting device to rotate, so that the supporting device is rotated. The supporting device drives the multiple sets of second blades 108 to move circumferentially, so that the multiple sets of first blades 107 and the multiple sets of second blades 108 mix and stir the materials in the tank body 101 into an emulsion. At the same time, the circulation device extracts the emulsion at the bottom of the tank body 101 and refluxes it upward into the tank body 101, thereby improving the uniform preparation effect of the circulation of the emulsion. When the emulsion is stirred, the multiple sets of second blades 108 are driven by the supporting device to vibrate up and down, so that the multiple sets of second blades 108 cooperate with the first blades 107 on both sides to shear the emulsion during the up and down movement. At the same time, the ultrasonic transducer 105 is turned on to improve the crushing of the emulsion. Effect: when the emulsion is prepared, the calcium chloride solution is added into the tank body 101 through the supporting device, and the materials are continuously stirred by the first blade 107 and the second blade 108, so that the sodium alginate and the calcium chloride react fully to form a calcium alginate shell layer and embed the fish oil, and obtain a nano-scale fish oil-carrying calcium alginate microcapsule suspension, thereby improving the multi-purpose effect of the equipment, reducing the complexity of equipment replacement during the preparation process, and improving the preparation efficiency and convenience; after the frame body 106 rotates, the spline sleeve 201 is driven to rotate, and after the spline sleeve 201 rotates, the hollow shaft 202 is driven to rotate, so that the hollow shaft 202 drives the second blade 108 to move circumferentially, Then, the multiple groups of second blades 108 and the multiple groups of first blades 107 are synchronously moved circumferentially, and the vibration motor 206 is turned on, so that the vibration motor 206 drives the top plate 203 to vibrate up and down through the multiple groups of guide columns 204 and the multiple groups of springs 205, so that the top plate 203 drives the hollow shaft 202 to vibrate up and down, and the hollow shaft 202 drives the multiple groups of second blades 108 to vibrate up and down to shear and crush the emulsion in the tank body 101, and the calcium chloride solution is transported to the inside of the hollow shaft 202 through the conveying device, so that the hollow shaft 202 adds the calcium chloride solution into the tank body 101 through the discharge port to mix with the emulsion, thereby improving the convenience of microcapsule preparation.

[0061] like Figures 1 to 12As shown, a method for preparing nano-sized fish oil-carrying calcium alginate microcapsules of the present invention comprises the following steps: during operation, a sodium alginate aqueous solution is placed into a tank body 101 through a first group of input ends of a first three-way pipe 102, and then the sodium alginate aqueous solution in the tank body 101 is heated by an electric heater 104, and then the antioxidant fish oil is transported into the tank body 101 through a second group of input ends of the first three-way pipe 102, and at the same time, a frame 106 is driven to rotate by a driving device, and after the frame 106 rotates, multiple groups of first blades 107 are driven to move circumferentially, and at the same time, the frame 106 drives a supporting device to rotate, so that the supporting device drives multiple groups of second blades 108 to move circumferentially, so that multiple groups of first blades 107 and multiple groups of second blades 108 move the inside of the tank body 101. The materials are mixed and stirred into an emulsion, and at the same time, the circulation device extracts the emulsion at the bottom of the tank body 101 and refluxes it upward into the tank body 101. When the emulsion is stirred, the supporting device drives the multiple sets of second blades 108 to vibrate up and down, so that the multiple sets of second blades 108 cooperate with the first blades 107 on both sides to shear the emulsion during the up and down movement. At the same time, the ultrasonic transducer 105 is turned on to improve the crushing effect of the emulsion. When the emulsion is prepared, the calcium chloride solution is added into the tank body 101 through the supporting device, and the materials are continuously stirred by the first blade 107 and the second blade 108, so that the sodium alginate and the calcium chloride are fully reacted to form a calcium alginate shell layer and embed the fish oil, so as to obtain a nano-scale fish oil-carrying calcium alginate microcapsule suspension.

[0062] The main functions achieved by the present invention are: reducing the complexity of converting emulsions to different equipment in different steps, reducing production costs and material losses, making the equipment easy to operate and control, and improving work efficiency. By adopting a homogenizing device to stir, mix and shear emulsify the sodium alginate aqueous solution with the antioxidant fish oil, the convenience of preparing nano-sized calcium alginate microcapsules loaded with fish oil is improved, and the equipment maintenance cost is reduced.

[0063] The electric heater 104, ultrasonic transducer 105, vibration motor 206, transfer case 302, drive motor 303, clutch 305 and pump body 501 of the method for preparing nano-particle size fish oil calcium alginate microcapsules of the present invention are purchased on the market. Technicians in the industry only need to install and operate them according to the accompanying instruction manual, without the need for creative labor by technicians in this field.

[0064] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules, characterized in that: S1, the fish oil collected is preliminarily cleaned and a skimmer is used to remove floating dirt, and then the fish oil is filtered step by step using filters of different mesh sizes, first using a 90-mesh filter to filter once, and then using 150-mesh and 250-mesh filters to filter the fish oil again in sequence; S2, removing excess water from the filtered fish oil by vacuum drying, so that the viscosity of the fish oil is 10-4 to 10-7 Pa·s at a temperature of 23-28°C; S3, adding 0.02%-0.05% antioxidant to the fish oil in S2, stirring with a magnetic stirrer until completely dissolved, to obtain antioxidant fish oil; S4, dissolving sodium alginate in deionized water and heating to 55-60° C. to make the concentration of sodium alginate 1.0%-2.0%, slowly adding emulsifier and stabilizer during heating, and stirring well to obtain sodium alginate aqueous solution; S5, first put the sodium alginate aqueous solution into the homogenizing device, then slowly add the antioxidant fish oil into the homogenizing device to stir and mix to form a stable emulsion, and the temperature in the homogenizing device is controlled at 23-28°C; S6. In S5, the emulsion is sheared and ultrasonically vibrated by a homogenizing device, and the emulsion is made to reach the nanometer level by utilizing the cavitation effect and shear mechanical effect of the ultrasonic wave; S7, slowly adding the calcium chloride solution to the nanoemulsion in S6, stirring while adding, so that the sodium alginate and the calcium chloride fully react to form a calcium alginate shell layer and embed the fish oil, to obtain a nanoscale fish oil-loaded calcium alginate microcapsule suspension; S8, centrifuging the nanoscale fish oil-loaded calcium alginate microcapsule suspension to remove excess unreacted wall material, and then drying it; S9. Screening the dried microcapsules to obtain fish oil-loaded calcium alginate microcapsule products with nano-scale particle sizes that meet the requirements.

2. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 1, characterized in that: In S5, the sodium alginate aqueous solution is placed in a homogenizing device and preheated to 23-28° C., and the antioxidant fish oil is maintained at a temperature of 23-28° C. and slowly added to the homogenizing device.

3. The method for preparing nano-sized fish oil-loaded calcium alginate microcapsules according to claim 1, characterized in that: The homogenizing device comprises a supporting device, a circulating device, a driving device, a tank body (101), a first three-way pipe (102), two groups of first valves (103), an electric heater (104), an ultrasonic transducer (105), a frame (106), a plurality of groups of first blades (107) and a plurality of groups of second blades (108); the first three-way pipe (102) is arranged on the outer wall of the tank body (101) for communication; the two groups of first valves (103) are arranged on two groups of input ends of the first three-way pipe (102) for communication, the electric heater (104) and the ultrasonic transducer (105) are respectively installed on the inner wall of the tank body (101); the frame (106) is rotatable The plurality of first blades (107) are mounted on the inner wall of the tank body (101), the plurality of first blades (107) are mounted on the outer wall of the frame body (106), the plurality of second blades (108) are mounted on the supporting device, the supporting device is used to drive the plurality of second blades (108) to vibrate up and down, and the supporting device is used to transport the calcium chloride solution into the tank body (101), the plurality of second blades (108) and the plurality of first blades (107) are arranged in an alternating manner, the circulation device is arranged on the tank body (101), the circulation device is used to circulate the material in the tank body (101), and the driving device is arranged on the tank body (101), the driving device is used to drive the frame body (106) to rotate.

4. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 3, characterized in that: The supporting device comprises a conveying device, a spline sleeve (201), a hollow shaft (202), a top plate (203), multiple groups of guide posts (204), multiple groups of springs (205) and a vibration motor (206); the spline sleeve (201) is mounted on the outer wall of the frame (106); the lower part of the hollow shaft (202) is mounted on the spline sleeve (201) by sliding up and down through a spline; the upper part of the hollow shaft (202) is rotatably mounted on the top plate (203); the top end of the hollow shaft (202) is connected to the conveying device, and the conveying device is used to transfer the calcium chloride solution The liquid is transported to the inside of the hollow shaft (202); an outer wall of the hollow shaft (202) is provided with an outlet, which is communicated with the inside of the tank body (101); multiple groups of guide columns (204) are installed on the top of the tank body (101); a top plate (203) is installed on the multiple groups of guide columns (204) to slide up and down; multiple groups of springs (205) are respectively mounted on the outer walls of the multiple groups of guide columns (204); a vibration motor (206) is installed on the outer wall of the top plate (203); and multiple groups of second blades (108) are installed on the outer wall of the hollow shaft (202).

5. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 3, characterized in that: The driving device comprises a gear ring (301), a transfer case (302), a driving motor (303), a gear (304), a clutch (305), a disc (306), a connecting rod (307), a cylinder (308) and a one-way valve (309); the gear ring (301) is mounted on the outer wall of the frame (106); the transfer case (302) is mounted on the inner wall of the tank (101); the driving motor (303) is mounted on the outer wall of the transfer case (302); the output end of the driving motor (303) is connected to the transfer case (302); the gear (304) is mounted on the first output end of the transfer case (302); The gear (304) is meshed with the gear ring (301), the clutch (305) is connected to the second output end of the transfer case (302), the disc (306) is mounted on the output end of the clutch (305), one end of the connecting rod (307) is rotationally connected to the outer wall of the disc (306) at an eccentric position, the other end of the connecting rod (307) is rotationally connected to the piston, the piston is slidably arranged inside the cylinder (308), the cylinder (308) is mounted on the outer wall of the tank (101), two groups of one-way valves (309) are respectively connected and arranged on the outer wall of the cylinder (308), and one group of the one-way valves (309) is connected to the conveying device.

6. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 4, characterized in that: The conveying device comprises a connecting device, an air bag (401), a storage tank (402), a second valve (403) and a third valve (404); the air bag (401) is connected to one of the one-way valves (309); a feed valve is arranged on the outer wall of the storage tank (402); the storage tank (402) is connected to the air bag (401) through the second valve (403); the third valve (404) is arranged on the storage tank (402); the bottom end of the third valve (404) extends into the bottom of the storage tank (402) through a pipeline; and the output end of the third valve (404) is connected to the hollow shaft (202) through the connecting device.

7. The method for preparing nano-sized fish oil-loaded calcium alginate microcapsules according to claim 3, characterized in that: The circulation device comprises a transmission device, a pump body (501), a second three-way pipe (502), two groups of fourth valves (503) and a delivery pipe (504); the pump body (501) is mounted on the outer wall of the tank body (101); the power input end of the pump body (501) is connected to the gear ring (301) through the transmission device; the second three-way pipe (502) is arranged in communication with the output end of the pump body (501); one group of output ends of the second three-way pipe (502) is in communication with the tank body (101); the delivery pipe (504) is arranged in communication with the input end of the pump body (501); the input end of the delivery pipe (504) is arranged in communication with the lower part of the tank body (101); and the two groups of fourth valves (503) are respectively arranged in communication with the two groups of output ends of the second three-way pipe (502).

8. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 6, characterized in that: The connecting device comprises a hose (601) and a conveying box (602); the input end of the hose (601) is connected to the output end of the third valve (404); the output end of the hose (601) is connected to the conveying box (602); the conveying box (602) is mounted on the top of the top plate (203); the upper portion of the hollow shaft (202) is rotated to extend into the interior of the conveying box (602); the upper portion of the hollow shaft (202) is provided with an inlet, which is connected to the interior of the conveying box (602).

9. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 7, characterized in that: The transmission device comprises two groups of pulleys (701) and belts (702), wherein the first group of pulleys (701) is mounted on the outer wall of the gear ring (301), and the second group of pulleys (701) is mounted on the power input end of the pump body (501), and the belts (702) are sleeved on the outer walls of the two groups of pulleys (701).

10. The method for preparing nanometer-sized fish oil-loaded calcium alginate microcapsules according to claim 4, characterized in that: It also includes a plurality of groups of nozzles (801), and the plurality of groups of nozzles (801) are all connected and arranged at the discharge outlet of the hollow shaft (202).

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