Animal food microbiological detection sterilization device and detection method thereof

Abstract

The invention discloses an animal food microorganism detection sterilization device and a detection method thereof.The animal food microorganism detection sterilization device comprises a main machine body, the front side of the main machine body is fixedly connected with a sterilization box, a Y-axis conveying device is arranged in the main machine body and the sterilization box, and the upper portion of a conveying plate of the Y-axis conveying device is fixedly connected with an X-axis output device; a culture tray is arranged at the upper part of a conveying plate of the X-axis output device. According to the present invention, the main machine body, the sterilization box body, the Y-axis conveying device, the X-axis output device and the culture disc are arranged, and the roller stirring device, the liquid dropping joint, the detection head, the upper box body, the reagent storage box, the first quantitative tube, the culture solution storage box, the second quantitative tube, the crushing device and other components are arranged; the integrated operation from sample treatment to detection result output is realized, the microorganism detection process is simplified, the manual operation is reduced, the detection efficiency is improved, and finally, the effect of efficiently, quickly and automatically detecting microorganisms in animal food is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial detection, and in particular relates to an animal food microbial detection and sterilization device and a detection method thereof. Background Art

[0002] Microbial contamination is one of the important issues in food safety, especially in animal foods. Traditional microbial detection methods usually include sample processing, culture medium preparation, inoculation, cultivation, observation and identification. The whole process is time-consuming, cumbersome, and easily affected by environmental factors. In recent years, with the development of automation technology and biosensor technology, a variety of automated microbial detection equipment have emerged. These devices can realize rapid processing and detection of samples, improving detection efficiency and accuracy. However, the existing automated microbial detection systems still have certain limitations. For example, the cell structure may not be effectively broken during sample processing, thus affecting the subsequent microbial detection results; in addition, the risk of cross-contamination during sample processing and cultivation still exists, especially when dealing with different types of animal foods. How to ensure a sterile environment between each test becomes a major challenge.

[0003] Although the microbial detection equipment currently on the market can achieve a certain degree of automation, manual operation or semi-automatic equipment is often used in the sample pretreatment stage, which is not only inefficient but also prone to human errors. Especially when dealing with animal food samples, since such foods may contain a large amount of fat, protein and other complex organic components, traditional detection methods are often difficult to completely separate and enrich the target microorganisms, resulting in reduced reliability of the test results. In addition, most of the microbial detection devices in the prior art lack effective sterilization functions, especially the failure to completely remove residual microorganisms between multiple uses, which undoubtedly increases the risk of cross-contamination and reduces the credibility of the test results. Therefore, how to design a device that can efficiently and accurately complete animal food microbial detection and effectively avoid cross-contamination has become an urgent problem to be solved. Therefore, we propose an animal food microbial detection sterilization device and a detection method thereof. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide an animal food microorganism detection and sterilization device and a detection method thereof. By setting a main body, a sterilization box, a Y-axis conveying device, an X-axis output device and a culture plate, and equipped with a roller stirring device, a drip joint, a detection head, an upper box, a reagent storage box, a first quantitative tube, a culture fluid storage box, a second quantitative tube and a crushing device and other components, an integrated operation from sample processing to test result output is realized, which simplifies the microorganism detection process, reduces manual operation and improves detection efficiency.

[0005] The present invention is implemented as follows: an animal food microorganism detection and sterilization device and a detection method thereof, comprising a main body, the front side of the main body is fixedly connected to a sterilization box, the main body and the sterilization box are provided with a Y-axis conveying device inside, the upper part of the conveying plate of the Y-axis conveying device is fixedly connected to an X-axis output device, and the upper part of the conveying plate of the X-axis output device is provided with a culture plate; the main body is fixedly connected to the inside, the upper part of the partition is provided with a roller stirring device, the lower part of the roller stirring device is connected to a plurality of drip joints, and the lower part of the partition is provided with a detection head ; An upper box body is fixedly connected to the top of the main body, and a plurality of reagent storage boxes are arranged inside the upper box body, and a first quantitative tube is connected to the lower part of the reagent storage box, and the first quantitative tube is connected to the drum stirring device through a bent pipe; a plurality of culture fluid storage boxes are arranged inside the upper box body, and a second quantitative tube is connected to the lower part of the culture fluid storage box, and a dropper is connected to the lower part of the second quantitative tube; a crushing device is arranged inside the upper box body, and the lower part of the crushing device is connected to the drum stirring device through a connecting pipe, and a third solenoid valve is arranged on the upper part of the connecting pipe.

[0006] Optionally, the drum stirring device includes an outer cylinder and an inner cylinder, and a first motor is fixedly connected to the side wall of the main body, and the first motor is used to drive the inner cylinder to rotate.

[0007] Optionally, the crushing device includes a shell, a second motor is fixedly connected to the side wall of the upper box body, a blade is provided inside the shell, and the second motor drives the blade to rotate via a rotating shaft.

[0008] Optionally, a cover is provided on the upper portion of the crushing device, a hook is fixedly connected to the side of the cover, and a groove for connecting with the hook is provided on the inner wall of the crushing device.

[0009] Optionally, a mounting plate is fixedly connected to the lower portion of the cover, a mounting groove is provided at the lower portion of the mounting plate, a rebound plate is detachably connected to the interior of the mounting groove, and an inclined plate is fixedly connected to the lower portion of the rebound plate.

[0010] Optionally, a transparent protective plate is fixedly connected to the front side of the main body, a bottom plate is fixedly connected to the lower part of the main body, a cabinet door is connected to the front side of the sterilization box, and a plurality of switches are provided on the transparent protective plate.

[0011] Optionally, the upper parts of the reagent storage box and the culture fluid storage box are rotatably connected with antibacterial covers respectively.

[0012] Optionally, a first solenoid valve is installed at the upper part and the lower part of the first quantitative tube, respectively, and a second solenoid valve is installed at the upper part and the lower part of the second quantitative tube, respectively.

[0013] Optionally, the upper and lower parts of the drum stirring device are respectively fixedly connected with shock-absorbing blocks, the inner top of the sterilization box is fixedly connected with an ultraviolet lamp tube, the upper part of the drum stirring device is fixedly connected with a water pump, and the water pump is connected with a drain pipe.

[0014] The present invention also discloses a method for detecting animal food microorganisms, including the animal food microorganism detection and sterilization device, comprising the following steps:

[0015] S1. Add the sample to be tested and distilled water into the crushing device, turn on the switch of the second motor, and the output shaft of the second motor drives the blade to rotate to crush the sample for - minutes to prepare a sample solution;

[0016] S2, opening the third solenoid valve to allow the sample solution to enter the drum stirring device through the connecting tube, and the first motor drives the inner drum to rotate. During this process, the reagent in the reagent storage box is quantitatively passed through the first quantitative tube and then flows into the drum stirring device, and is mixed with the sample solution to obtain a sample for dripping;

[0017] S3, the sample for dripping is dripped into the interior of the culture plate through the dripping joint, during which the X-axis output device and the Y-axis conveying device can drive the culture plate to move, so that each culture tank has the same volume of the sample for dripping;

[0018] S4, the X-axis output device and the Y-axis conveying device can drive the culture plate to move to the inside of the sterilization box, and the ultraviolet lamp can remove the bacteria;

[0019] S5, turn off the ultraviolet lamp, and the culture solution in the culture solution storage box is quantitatively measured by the second quantitative tube and then dripped into each culture tank of the culture plate, culture-H;

[0020] S6. Detection is performed through the detection head. During this process, the X-axis output device and the Y-axis conveying device can drive the culture plate to move, so that each culture tank moves to the bottom of the detection head in turn.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. By setting up a main body, a sterilization box, a Y-axis conveying device, an X-axis output device and a culture plate, and equipping with a roller stirring device, a drip joint, a detection head, an upper box, a reagent storage box, a first quantitative tube, a culture solution storage box, a second quantitative tube and a crushing device and other components, an integrated operation from sample processing to test result output is realized. The function and use are to simplify the microbial detection process, reduce manual operations, and improve detection efficiency, and finally achieve the effect of efficient, rapid and automated detection of microorganisms in animal food, while also reducing the risk of cross contamination.

[0023] 2. By adopting an outer cylinder and an inner cylinder structure, and driving the inner cylinder to rotate by the first motor, the uniformity and sufficiency of mixing of the sample and the reagent are achieved, ensuring that the microorganisms in the sample can be fully extracted and dispersed, and ultimately achieving the beneficial effect of improving the accuracy and reliability of the test results.

[0024] 3. By setting up a crushing device with a blade, and driving the blade to rotate through a second motor, combined with the rebound plate and inclined plate design, the sample can be effectively crushed and ground, which is convenient for subsequent microbial extraction and detection, and ultimately achieves the effect of improving sample processing efficiency and quality. In addition, the hook on the cover and the groove design on the crushing device ensure the stability and safety of the device.

[0025] Further features and advantages of the present invention will become apparent from the following detailed description of exemplary embodiments of the present invention with reference to the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] FIG1 is a schematic diagram of a first viewing angle structure provided by the present invention;

[0027] FIG2 is a schematic diagram of a second viewing angle provided by the present invention;

[0028] FIG3 is a schematic diagram of a drum stirring device provided by the present invention;

[0029] FIG4 is a schematic diagram of a second quantitative tube provided by the present invention;

[0030] FIG5 is a schematic diagram of the interior of a drum stirring device provided by the present invention;

[0031] FIG6 is a schematic diagram of a drip joint provided by the present invention;

[0032] Figure 7 is a schematic diagram of a blade provided by the present invention;

[0033] Figure 8 is a schematic diagram of the installation slot provided by the present invention;

[0034] Fig. 9 The present invention provides Figure 8 The enlarged schematic diagram at A in the middle;

[0035] Fig.10 It is a schematic diagram of the drainage pipe provided by the present invention.

[0036] In the figure: 1. main body; 2. upper box; 3. crushing device; 4. cover; 5. transparent protective plate; 6. cabinet door; 7. sterilization box; 8. culture solution storage box; 9. second motor; 10. first motor; 11. partition; 12. drum stirring device; 13. shock-absorbing block; 14. first quantitative tube; 15. first solenoid valve; 16. dropper; 17. ultraviolet lamp; 18. bottom plate; 19. second solenoid valve; 20. second quantitative tube; 21. reagent storage box; 22. culture plate; 23. X-axis output device; 24. Y-axis conveying device; 25. blade; 26. mounting plate; 27. mounting groove; 28. rebound plate; 29. ​​connecting pipe; 30. drain pipe; 31. hook; 32. antibacterial cover; 33. drip connector; 34. detection head. DETAILED DESCRIPTION

[0037] In order to further understand the content, features and effects of the present invention, the following embodiments are given as examples and described in detail with reference to the accompanying drawings.

[0038] As shown in Figure 1 to Fig.10 As shown, an embodiment of the present invention provides an animal food microorganism detection and sterilization device and a detection method thereof.

[0039] The main body 1 comprises a sterilization box 7 fixedly connected to the front side of the main body 1, a Y-axis conveying device 24 is arranged inside the main body 1 and the sterilization box 7, an X-axis output device 23 is fixedly connected to the upper part of the conveying plate of the Y-axis conveying device 24, and a culture plate 22 is arranged on the upper part of the conveying plate of the X-axis output device 23; a partition 11 is fixedly connected inside the main body 1, a drum stirring device 12 is arranged on the upper part of the partition 11, a plurality of dripping joints 33 are connected to the lower part of the drum stirring device 12, and a detection head 34 is arranged on the lower part of the partition 11; an upper portion of the main body 1 is fixedly connected to the top The box body 2 includes a plurality of reagent storage boxes 21 disposed inside the upper box body 2, the lower part of the reagent storage box 21 is connected to a first quantitative tube 14, and the first quantitative tube 14 is connected to the drum stirring device 12 through a bent pipe; the upper box body 2 includes a plurality of culture fluid storage boxes 8, the lower part of the culture fluid storage box 8 is connected to a second quantitative tube 20, and the lower part of the second quantitative tube 20 is connected to a dropper 16; the upper box body 2 includes a crushing device 3, the lower part of the crushing device 3 is connected to the drum stirring device 12 through a connecting pipe 29, and the upper part of the connecting pipe 29 is provided with a third solenoid valve.

[0040] The drum stirring device 12 includes an outer drum and an inner drum. A first motor 10 is fixedly connected to the side wall of the main body 1. The first motor 10 is used to drive the inner drum to rotate.

[0041] The crushing device 3 comprises a shell, a second motor 9 is fixedly connected to the side wall of the upper box body 2, a blade 25 is arranged inside the shell, and the second motor 9 drives the blade 25 to rotate via a rotating shaft.

[0042] A cover 4 is provided on the upper part of the crushing device 3 , a hook 31 is fixedly connected to the side of the cover 4 , and a groove for connecting with the hook 31 is provided on the inner wall of the crushing device 3 .

[0043] A mounting plate 26 is fixedly connected to the lower part of the cover 4. A mounting groove 27 is provided at the lower part of the mounting plate 26. A rebound plate 28 is detachably connected inside the mounting groove 27. An inclined plate is fixedly connected to the lower part of the rebound plate 28.

[0044] A transparent protective plate 5 is fixedly connected to the front side of the main body 1 , a bottom plate 18 is fixedly connected to the lower part of the main body 1 , a cabinet door 6 is connected to the front side of the sterilization box 7 , and a plurality of switches are arranged on the transparent protective plate 5 .

[0045] The upper parts of the reagent storage box 21 and the culture solution storage box 8 are rotatably connected with antibacterial covers 32 respectively.

[0046] The first electromagnetic valve 15 is installed at the upper part and the lower part of the first quantitative tube 14 , respectively. The second electromagnetic valve 19 is installed at the upper part and the lower part of the second quantitative tube 20 , respectively.

[0047] The upper and lower parts of the drum stirring device 12 are respectively fixedly connected with shock absorbing blocks 13, the inner top of the sterilization box 7 is fixedly connected with an ultraviolet lamp tube 17, the upper part of the drum stirring device 12 is fixedly connected with a water pump, and the water pump is connected with a drainage pipe 30.

[0048] The present invention also discloses a method for detecting animal food microorganisms, including an animal food microorganism detection and sterilization device, comprising the following steps:

[0049] S1. Add the sample to be tested and distilled water into the crushing device 3, turn on the switch of the second motor 9, and the output shaft of the second motor 9 drives the blade 25 to rotate, crushing the sample for 2-10 minutes to prepare a sample solution;

[0050] S2, open the third solenoid valve to allow the sample solution to enter the drum stirring device 12 through the connecting tube 29, and the first motor 10 drives the inner drum to rotate. During this process, the reagent in the reagent storage box 21 is quantitatively passed through the first quantitative tube 14 and flows into the drum stirring device 12, and is mixed with the sample solution to obtain a sample for dripping;

[0051] S3, the sample for dripping is dripped into the interior of the culture plate 22 through the dripping joint 33. During this process, the X-axis output device 23 and the Y-axis conveying device 24 can drive the culture plate 22 to move, so that each culture tank has the same volume of the sample for dripping;

[0052] S4, the X-axis output device 23 and the Y-axis conveying device 24 can drive the culture plate 22 to move to the inside of the sterilization box 7, and the ultraviolet lamp 17 can remove the bacteria;

[0053] S5, turn off the ultraviolet lamp 17, and the culture solution in the culture solution storage box 8 is quantitatively measured by the second quantitative tube 20 and then dripped into each culture tank of the culture plate 22 for 1-12 hours;

[0054] S6. Detection is performed through the detection head 34. During this process, the X-axis output device 23 and the Y-axis conveying device 24 can drive the culture plate 22 to move, so that each culture tank moves to the bottom of the detection head 34 in turn.

[0055] Working principle: In the traditional microbiological detection process, the sample handling usually requires manual operation, which is not only time-consuming but also easy to introduce errors. The present invention realizes the precise movement of the sample in three-dimensional space by combining the Y-axis conveying device 24 and the X-axis output device 23. This design overcomes the problem of inaccurate positioning caused by manual handling in the traditional method, improves the accuracy of sample positioning, and thus enhances the reliability of the detection results.

[0056] The drum stirring device 12 includes an outer cylinder and an inner cylinder, and the inner cylinder is driven to rotate by the first motor 10. Compared with the common single-layer stirring method, this double-layer structure design can effectively prevent the sample from adhering to the container wall while ensuring uniform mixing of the sample, thereby avoiding sample loss. In addition, by controlling the rotation speed of the inner cylinder through the motor, the stirring intensity can be adjusted according to the needs of different samples, thereby enhancing the adaptability and flexibility of the device.

[0057] The crushing device 3 uses the second motor 9 to drive the blade 25 to crush the sample. Compared with the traditional manual or simple mechanical crushing, the present invention adopts a design with a rebound plate 28 and an inclined plate, which not only increases the chance of contact between the sample and the blade 25 and improves the crushing efficiency, but also the design of the rebound plate 28 can prevent large particles from blocking the outlet, ensuring smooth operation under continuous working conditions. In addition, the hook 31 on the cover 4 is used in conjunction with the groove on the crushing device 3, which simplifies the assembly process of the device and facilitates daily maintenance and cleaning.

[0058] The ultraviolet lamp 17 in the sterilization box 7 is used to disinfect the culture environment to prevent external microbial contamination from affecting the test results. The water pump and drain pipe 30 fixed on the upper part of the drum stirring device 12 constitute a complete drainage system, which can automatically remove residues after the test is completed, reducing manual intervention, ensuring the sanitary conditions of the equipment, and reducing the risk of cross infection.

[0059] In summary, the design of the above key steps innovatively solves the deficiencies in the prior art, such as inaccurate sample handling, uneven mixing, low crushing efficiency, and inconvenient sanitary maintenance, thereby significantly improving the accuracy and efficiency of microbial detection. These improvements work together to make the present invention an advanced animal food microbial detection and sterilization device that integrates high efficiency, accuracy, and safety.

[0060] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An animal food microorganism detection and sterilization device, comprising a main body (1), characterized in that: The front side of the main body (1) is fixedly connected to a sterilization box (7); a Y-axis conveying device (24) is provided inside the main body (1) and the sterilization box (7); an X-axis output device (23) is fixedly connected to the upper part of the conveying plate of the Y-axis conveying device (24); and a culture tray (22) is provided on the upper part of the conveying plate of the X-axis output device (23); A partition (11) is fixedly connected to the interior of the main body (1), a drum stirring device (12) is provided on the upper part of the partition (11), a plurality of dripping joints (33) are connected to the lower part of the drum stirring device (12), and a detection head (34) is provided on the lower part of the partition (11); The top of the main body (1) is fixedly connected to an upper box (2), a plurality of reagent storage boxes (21) are arranged inside the upper box (2), a first quantitative tube (14) is connected to the bottom of the reagent storage box (21), and the first quantitative tube (14) is connected to the drum stirring device (12) through a curved tube; A plurality of culture solution storage boxes (8) are provided inside the upper box body (2); a second quantitative tube (20) is connected to the lower part of the culture solution storage box (8); and a dropper (16) is connected to the lower part of the second quantitative tube (20); A crushing device (3) is provided inside the upper box body (2); the lower part of the crushing device (3) is connected to the drum stirring device (12) via a connecting pipe (29); and a third solenoid valve is provided on the upper part of the connecting pipe (29).

2. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: The drum stirring device (12) comprises an outer drum and an inner drum, and a first motor (10) is fixedly connected to the side wall of the main body (1), and the first motor (10) is used to drive the inner drum to rotate.

3. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: The crushing device (3) comprises a shell, a second motor (9) is fixedly connected to the side wall of the upper box body (2), a blade (25) is provided inside the shell, and the second motor (9) drives the blade (25) to rotate via a rotating shaft.

4. The device for detecting and sterilizing microorganisms of animal food according to claim 3, characterized in that: The upper part of the crushing device (3) is provided with a cover (4), the side of the cover (4) is fixedly connected with a hook (31), and the inner wall of the crushing device (3) is provided with a groove for connecting with the hook (31).

5. The device for detecting and sterilizing microorganisms of animal food according to claim 4, characterized in that: A mounting plate (26) is fixedly connected to the lower part of the cover (4), a mounting groove (27) is provided at the lower part of the mounting plate (26), a rebound plate (28) is detachably connected to the inside of the mounting groove (27), and a slanted plate is fixedly connected to the lower part of the rebound plate (28).

6. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: A transparent protective plate (5) is fixedly connected to the front side of the main body (1), a bottom plate (18) is fixedly connected to the lower part of the main body (1), a cabinet door (6) is connected to the front side of the sterilization box (7), and a plurality of switches are provided on the transparent protective plate (5).

7. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: The upper parts of the reagent storage box (21) and the culture fluid storage box (8) are respectively rotatably connected with antibacterial covers (32).

8. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: The upper and lower parts of the first quantitative tube (14) are respectively installed with a first solenoid valve (15), and the upper and lower parts of the second quantitative tube (20) are respectively installed with a second solenoid valve (19).

9. The device for detecting and sterilizing microorganisms of animal food according to claim 1, characterized in that: The upper and lower parts of the drum stirring device (12) are respectively fixedly connected to shock absorbing blocks (13); the inner top of the sterilization box (7) is fixedly connected to an ultraviolet lamp tube (17); the upper part of the drum stirring device (12) is fixedly connected to a water pump; and the water pump is connected to a drainage pipe (30).

10. A method for detecting microorganisms in animal food, comprising the device for detecting and sterilizing microorganisms in animal food according to any one of claims 1 to 9, characterized in that: It includes the following steps: S1. Add the sample to be tested and distilled water into the crushing device (3), turn on the switch of the second motor (9), and the output shaft of the second motor (9) drives the blade (25) to rotate, crushing the sample for 2-10 minutes to prepare a sample solution; S2, opening the third solenoid valve to allow the sample solution to enter the drum stirring device (12) through the connecting tube (29), and the first motor (10) drives the inner drum to rotate. During this process, the reagent in the reagent storage box (21) is quantitatively measured through the first quantitative tube (14) and then flows into the drum stirring device (12), where it is mixed with the sample solution to obtain a sample for dripping; S3, the sample for dripping is dripped into the interior of the culture plate (22) through the dripping joint (33), and during this process, the X-axis output device (23) and the Y-axis conveying device (24) can drive the culture plate (22) to move, so that each culture tank has the same volume of the sample for dripping; S4, the X-axis output device (23) and the Y-axis conveying device (24) can drive the culture plate (22) to move into the sterilization box (7), and the ultraviolet lamp tube (17) can remove miscellaneous bacteria; S5, turning off the ultraviolet lamp (17), and the culture solution in the culture solution storage box (8) is quantitatively measured by the second quantitative tube (20) and then dripped into each culture tank of the culture plate (22), and cultured for 1-12 hours; S6. Perform 3 detection through the detection head (34). During this process, the X-axis output device (23) and the Y-axis conveying device (24) can drive the culture plate (22) to move, so that each culture tank moves to the bottom of the detection head (34) in turn.

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