Breeding experiment pool for monitoring water quality by using zebra fish and monitoring method

Abstract

The invention relates to the technical field of biological monitoring, in particular to a culture experiment pool for monitoring water quality by using zebra fish and a monitoring method. Comprising a pool body, a water inlet pipe, a water outlet pipe, a fixing plate, a timer, a camera, a memory iron wire and an adjusting assembly, the adjusting assembly comprises a transverse moving module, a connecting frame, a driving piece, a movable frame, a circular shaft, a connecting rod and two telescopic rods, the transverse moving module is arranged on the outer side wall of the pool body, the connecting frame is connected with the transverse moving module, and the driving piece is fixedly connected with the connecting frame; one end of the connecting rod is connected with the driving piece, the circular shaft is connected with the other end of the connecting rod, the movable frame is movably connected with the circular shaft, the circular shaft is sleeved with the movable frame, the movable frame is further fixedly connected with the fixing plate, and the two ends of each telescopic rod are fixedly connected with the connecting frame and the movable frame respectively. The position and height of the camera can be adjusted according to monitoring requirements, the monitoring range of the camera is greatly enlarged, and stable water quality monitoring is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of biological monitoring, and in particular to a breeding experimental pond and a monitoring method for monitoring water quality using zebrafish. Background Art

[0002] Biological monitoring is often used to monitor industrial wastewater discharge and drinking water source water quality. The biological early warning system is a system that uses organisms as sensors to continuously monitor and warn of water pollution. This project uses zebrafish, which are sensitive to the toxicity of pollutants, as test fish. By observing the respiratory physiological changes of zebrafish, early warning of water source water quality can be achieved, thereby preliminarily evaluating the water quality of the water body. According to the above, because the biological water quality breeding experimental pools on the market are generally simple in structure and single in function, it is not only impossible to regularly test the water quality, but also inconvenient to effectively track and record the organisms, which ultimately affects the effect of water quality monitoring.

[0003] The prior art patent CN206586201U discloses a breeding experimental pool for water quality monitoring using zebrafish, including a pool body, an inlet pipe, a first solenoid valve, an outlet pipe, a second solenoid valve, a connecting plate, a fixing plate, a timer, a fixing seat, a memory wire, and a camera. The water body flows into the detection pool, and then the staff can put the zebrafish into the detection pool. At this time, the water body is detected by the zebrafish that is sensitive to the toxicity of pollutants, that is, the water quality of the water body is preliminarily evaluated. At the same time, the water in the pool body is discharged to the outside through the outlet pipe by opening the second solenoid valve. Finally, through the above method, the water body required for detection from the outside can be regularly allowed to enter the pool body, thereby realizing the timed detection of the water body. In addition, the staff can also open the camera and then pull the camera as needed, so that the camera drives the memory wire to move. Because the memory wire has a good shaping effect, it can drive the camera to move to the position required by the staff and fix it. Then, the zebrafish can be photographed by the camera. Finally, through the above method, the tracking and recording of the behavior of the zebrafish can be realized, which is convenient for the subsequent research and analysis of the staff.

[0004] However, in the above-mentioned existing method, the camera can only be adjusted in a short distance by the memory wire, and the monitoring range of the camera is relatively low, which is not conducive to the stable monitoring of water quality. Summary of the invention

[0005] The purpose of the present invention is to provide a breeding experimental pond and a monitoring method for water quality monitoring using zebrafish, aiming to solve the technical problem in the prior art that the camera can only be adjusted over a short distance by using a memory wire, the monitoring range of the camera is low, and it is not conducive to the stable monitoring of water quality.

[0006] To achieve the above-mentioned purpose, the present invention adopts a breeding experimental pool for monitoring water quality using zebrafish, comprising a pool body, a water inlet pipe, a water outlet pipe, a fixing plate, a timer, a camera, a memory iron wire and an adjustment component, wherein the water inlet pipe and the water outlet pipe are respectively connected to the pool body, the adjustment component is arranged on the outer side wall of the pool body, the fixing plate is connected to the adjustment component, the timer is arranged on the outer side wall of the fixing plate, and the two ends of the memory iron wire are respectively connected to the camera and the fixing plate;

[0007] The adjustment assembly includes a transverse movement module, a connecting frame, a driving member, a movable frame, a circular shaft, a connecting rod and two telescopic rods. The transverse movement module is arranged on the outer side wall of the pool body, the connecting frame is connected to the transverse movement module and is located above the transverse movement module, the driving member is fixedly connected to the connecting frame and is located above the connecting frame, one end of the connecting rod is connected to the driving member, the circular shaft is connected to the other end of the connecting rod, the movable frame is movably connected to the circular shaft and is sleeved on the outside of the circular shaft, the movable frame is also fixedly connected to the fixed plate and is located below the fixed plate, and the two ends of each of the telescopic rods are respectively fixedly connected to the connecting frame and the movable frame.

[0008] Wherein, the adjustment component also includes a support spring, and two ends of the support spring are fixedly connected to the connecting frame and the movable frame respectively.

[0009] Wherein, the driving member includes a support seat and a first motor, the support seat is fixedly connected to the connecting frame and is located above the connecting frame, the first motor is fixedly connected to the support seat and is located inside the support seat, and one end of the connecting rod is fixedly connected to the output end of the first motor.

[0010] Wherein, the telescopic rod includes an inner rod and an outer tube, the outer tube is fixedly connected to the connecting frame and is located above the connecting frame, one end of the inner rod is slidably connected to the outer tube, and the other end of the inner rod is fixedly connected to the movable frame.

[0011] Among them, the transverse movement module includes a mounting frame, a screw rod, a screw rod nut and a second motor. The mounting frame is fixedly connected to the pool body and is located on the outer side wall of the pool body. Both ends of the screw rod are rotatably connected to the mounting frame. The screw rod nut is sleeved on the outside of the screw rod and cooperates with the screw rod thread. The screw rod nut is also movably connected to the mounting frame and is located in the mounting frame. The second motor is arranged on the outer side wall of the mounting frame. The output end of the second motor is connected to the screw rod. The connecting frame is fixedly connected to the screw rod nut and is located above the screw rod nut.

[0012] Wherein, the breeding experimental pond for monitoring water quality using zebrafish also includes a handle, which is fixedly connected to the camera and is located on the outer side wall of the camera.

[0013] The present invention also provides a method for monitoring water quality using zebrafish, comprising the following steps:

[0014] conveying the monitored water body in the tank body;

[0015] The water inlet pipe and the water outlet pipe are used in conjunction with a solenoid valve to simulate the flow and changes of natural water bodies;

[0016] Zebrafish were placed in the tank;

[0017] The camera is installed above the pool body, and cooperates with a timer to regularly record the behavior changes of zebrafish;

[0018] Record water quality monitoring data and zebrafish behavior observations and generate detailed data reports.

[0019] The present invention provides a breeding experimental pond and a monitoring method for monitoring water quality using zebrafish. The water to be monitored flows into the pond, and then a staff member can put the zebrafish into the pond. Since zebrafish are sensitive to low-oxygen environments, they can quickly reflect changes in the oxygen content of the water quality. The camera is used to observe the health and behavioral changes of the zebrafish to obtain water quality monitoring data, thereby realizing the use of zebrafish to detect the water body. When adjusting the monitoring position of the camera, the lateral movement module is first used to drive the camera to lateral move to adjust the position, and then the driving member above the connecting frame is started, and the driving member controls the connecting rod to rotate, and the connecting rod will The circular shaft is driven to move in the movable frame, and the movable frame will be pulled downward or pushed upward by the circular shaft, so that the camera is raised or lowered, wherein the two telescopic rods will be extended and retracted according to the movement of the movable frame. After the camera height is adjusted appropriately, the driving member is stopped, and then the camera is used to observe the zebrafish in the pool. In addition, the camera can be pulled so that the camera drives the memory wire to move, so that the position of the camera can be fine-tuned. Through the above method, the position and height of the camera can be adjusted according to monitoring needs, which greatly increases the monitoring range of the camera and is conducive to the stable monitoring of water quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a structural schematic diagram of a breeding experimental pond for monitoring water quality using zebrafish according to the present invention.

[0022] Figure 2 It is a structural schematic diagram of another angle of the breeding experimental pond for monitoring water quality using zebrafish according to the present invention.

[0023] Figure 3 It is a structural front view of a breeding experimental pond for monitoring water quality using zebrafish according to the present invention.

[0024] Figure 4 The present invention is a structural side view of a breeding experimental pond for monitoring water quality using zebrafish.

[0025] Figure 5 The present invention is a top view of the structure of a breeding experimental pond for monitoring water quality using zebrafish.

[0026] Figure 6 It is a flow chart of the steps of the monitoring method for water quality monitoring using zebrafish of the present invention.

[0027] 101- pool body, 102- water inlet pipe, 103- water outlet pipe, 104- fixed plate, 105- timer, 106- camera, 107- memory wire, 108- connecting frame, 109- movable frame, 110- round shaft, 111- connecting rod, 112- supporting spring, 113- supporting seat, 114- first motor, 115- inner rod, 116- outer cylinder, 117- mounting frame, 118- lead screw, 119- lead screw nut, 120- second motor, 121- handle, 122- mounting frame, 123- sliding rod, 124- movable plate, 125- cleaning cotton, 126- observation window. DETAILED DESCRIPTION

[0028] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0029] See also Figure 1 to Figure 6The present invention provides a breeding experimental pond for water quality monitoring using zebrafish, comprising a pond body 101, a water inlet pipe 102, a water outlet pipe 103, a fixing plate 104, a timer 105, a camera 106, a memory wire 107 and an adjustment component, wherein the water inlet pipe 102 and the water outlet pipe 103 are respectively connected to the pond body 101, the adjustment component is arranged on the outer side wall of the pond body 101, the fixing plate 104 is connected to the adjustment component, the timer 105 is arranged on the outer side wall of the fixing plate 104, and the two ends of the memory wire 107 are respectively connected to the camera 106 and the fixing plate 104;

[0030] The adjustment assembly includes a transverse movement module, a connecting frame 108, a driving member, a movable frame 109, a circular shaft 110, a connecting rod 111 and two telescopic rods. The transverse movement module is arranged on the outer wall of the pool body 101, the connecting frame 108 is connected to the transverse movement module and is located above the transverse movement module, the driving member is fixedly connected to the connecting frame 108 and is located above the connecting frame 108, one end of the connecting rod 111 is connected to the driving member, the circular shaft 110 is connected to the other end of the connecting rod 111, the movable frame 109 is movably connected to the circular shaft 110 and is sleeved on the outside of the circular shaft 110, the movable frame 109 is also fixedly connected to the fixed plate 104 and is located below the fixed plate 104, and the two ends of each of the telescopic rods are respectively fixedly connected to the connecting frame 108 and the movable frame 109.

[0031] In this embodiment, the water to be monitored flows into the pool body 101, and then the staff can put the zebrafish into the pool body 101. Since zebrafish are sensitive to low-oxygen environments, they can quickly reflect changes in the oxygen content of the water quality. The camera 106 is used to observe the health and behavioral changes of the zebrafish to obtain water quality monitoring data, thereby realizing the use of zebrafish to detect the water body; when adjusting the monitoring position of the camera 106, the transverse movement module is first used to drive the camera 106 to move transversely to adjust the position, and then the driving member above the connecting frame 108 is started, and the driving member controls the rotation of the connecting rod 111, and the connecting rod 111 drives the circular shaft 110 to move in the movable frame 109 , the movable frame 109 will be pulled downward or pushed upward by the circular shaft 110, so that the camera 106 is raised or lowered, wherein the two telescopic rods will be extended and retracted according to the movement of the movable frame 109. After the height of the camera 106 is adjusted appropriately, the driving member is stopped, and then the zebrafish in the pool body 101 are observed using the camera 106. In addition, by pulling the camera 106, the camera 106 drives the memory wire 107 to move, so that the position of the camera 106 can be fine-tuned. Through the above method, the position and height of the camera 106 can be adjusted according to monitoring needs, which greatly increases the monitoring range of the camera 106 and is conducive to the stable monitoring of water quality.

[0032] Furthermore, the adjustment assembly further includes a support spring 112 , and two ends of the support spring 112 are fixedly connected to the connecting frame 108 and the movable frame 109 , respectively.

[0033] In this embodiment, the support spring 112 is provided, and the support spring 112 is located between the movable frame 109 and the connecting frame 108. The support spring 112 pulls the movable frame 109, thereby increasing the stability of the movable frame 109 when it is raised or lowered, and also preventing the movable frame 109 from falling rapidly due to equipment failure.

[0034] Furthermore, the driving member includes a support seat 113 and a first motor 114, the support seat 113 is fixedly connected to the connecting frame 108 and is located above the connecting frame 108, the first motor 114 is fixedly connected to the support seat 113 and is located inside the support seat 113, and one end of the connecting rod 111 is fixedly connected to the output end of the first motor 114.

[0035] In this embodiment, the support seat 113 fixedly supports the first motor 114 above the connecting frame 108, and the output end of the first motor 114 drives the connecting rod 111 to rotate, wherein the support seat 113 maintains a higher position above the connecting frame 108, enabling the connecting rod 111 to rotate in a circle.

[0036] Furthermore, the telescopic rod includes an inner rod 115 and an outer tube 116, the outer tube 116 is fixedly connected to the connecting frame 108 and is located above the connecting frame 108, one end of the inner rod 115 is slidably connected to the outer tube 116, and the other end of the inner rod 115 is fixedly connected to the movable frame 109.

[0037] In this embodiment, when the movable frame 109 moves up and down, the movable frame 109 drives the inner rod 115 to slide in the outer tube 116. The arrangement of the inner rod 115 and the outer tube 116 limits the moving trajectory of the movable frame 109 and its stability during movement.

[0038] Furthermore, the transverse movement module includes a mounting frame 117, a screw rod 118, a screw rod nut 119 and a second motor 120. The mounting frame 117 is fixedly connected to the pool body 101 and is located on the outer wall of the pool body 101. Both ends of the screw rod 118 are rotatably connected to the mounting frame 117. The screw rod nut 119 is sleeved on the outside of the screw rod 118 and threadedly cooperates with the screw rod 118. The screw rod nut 119 is also movably connected to the mounting frame 117 and is located in the mounting frame 117. The second motor 120 is arranged on the outer wall of the mounting frame 117. The output end of the second motor 120 is connected to the screw rod 118. The connecting frame 108 is fixedly connected to the screw rod nut 119 and is located above the screw rod nut 119.

[0039] In this embodiment, when the camera 106 is adjusted to move laterally, the second motor 120 is started, and the output end of the second motor 120 drives the screw rod 118 to rotate in the mounting frame 122. Since the screw nut 119 is threadedly matched with the screw rod 118, the screw nut 119 will move laterally in the mounting frame 122. Then, the screw nut 119 drives the camera 106 to move through the connecting frame 108. After the camera 106 is adjusted appropriately, the second motor 120 is stopped.

[0040] Furthermore, the aquaculture experimental pond for monitoring water quality using zebrafish also includes a handle 121 , which is fixedly connected to the camera 106 and is located on the outer side wall of the camera 106 .

[0041] In this embodiment, by providing the handle 121 on the camera 106 , the handle 121 can be used to facilitate fine adjustment of the position of the camera 106 .

[0042] Furthermore, the breeding experimental pond for water quality monitoring using zebrafish also includes an observation component, which includes an observation window 126, a mounting frame 122, a slide bar 123, a movable plate 124 and cleaning cotton 125. The observation window 126 is fixedly connected to the pool body 101 and is located on the pool body 101. The mounting frame 122 is fixedly connected to the pool body 101 and is located on the outer wall of the pool body 101. Both ends of the slide bar 123 are fixedly connected to the mounting frame 122. The movable plate 124 is slidably connected to the slide bar 123. The cleaning cotton 125 is fixedly connected to the movable plate 124 and is located on the inner wall of the movable plate 124.

[0043] In this embodiment, by setting the observation window 126 on the pool body 101, the staff can use the observation window 126 to visually understand the activities of the zebrafish in the pool body 101. When there is dust or dirt on the surface of the observation window 126, the movable plate 124 is pulled to slide back and forth on the slide rod 123 by applying force. At the same time, the movable plate 124 drives the cleaning cotton 125 to wipe the surface of the observation window 126, so that the observation window 126 is cleaned, which is convenient for visually observing the activities of the zebrafish in the pool body 101.

[0044] The present invention also provides a method for monitoring water quality using zebrafish, comprising the following steps:

[0045] S1: transporting the monitored water in the tank 101;

[0046] S2: Utilize the water inlet pipe 102 and the water outlet pipe 103 in conjunction with a solenoid valve to simulate the flow and changes of natural water bodies;

[0047] S3: placing zebrafish in the pool 101;

[0048] S4: Install the camera 106 above the pool 101 and cooperate with the timer 105 to regularly record the behavior changes of zebrafish;

[0049] S5: Record water quality monitoring data and zebrafish behavior observations and generate detailed data reports.

[0050] In this embodiment, zebrafish are sensitive to low oxygen environments and can quickly reflect changes in water quality oxygen content. First, the monitoring water is transported in the pool 101, and then the water inlet pipe 102 and the water outlet pipe 103 are used in conjunction with an electromagnetic valve to simulate the flow and changes of natural water. Zebrafish are then placed in the pool 101, and the camera 106 is installed above the pool 101. In conjunction with the timer 105, the behavior changes of zebrafish are recorded regularly, and water quality monitoring data and zebrafish behavior observation results are recorded in real time to generate a detailed data report. In the above-mentioned breeding monitoring system, by raising a zebrafish p65 gene knockout homozygous mutant strain, it is possible to monitor whether there is hypoxia in the water body, and the use of biological monitoring methods reduces chemical interference and pollution to the water body.

[0051] What is disclosed above is only a preferred embodiment of the present invention, and it certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.

Claims

1. A breeding experimental pond for water quality monitoring using zebrafish, characterized in that: It includes a pool body, a water inlet pipe, a water outlet pipe, a fixing plate, a timer, a camera, a memory iron wire and an adjustment component, wherein the water inlet pipe and the water outlet pipe are respectively connected to the pool body, the adjustment component is arranged on the outer side wall of the pool body, the fixing plate is connected to the adjustment component, the timer is arranged on the outer side wall of the fixing plate, and the two ends of the memory iron wire are respectively connected to the camera and the fixing plate; The adjustment assembly includes a transverse movement module, a connecting frame, a driving member, a movable frame, a circular shaft, a connecting rod and two telescopic rods. The transverse movement module is arranged on the outer side wall of the pool body, the connecting frame is connected to the transverse movement module and is located above the transverse movement module, the driving member is fixedly connected to the connecting frame and is located above the connecting frame, one end of the connecting rod is connected to the driving member, the circular shaft is connected to the other end of the connecting rod, the movable frame is movably connected to the circular shaft and is sleeved on the outside of the circular shaft, the movable frame is also fixedly connected to the fixed plate and is located below the fixed plate, and the two ends of each of the telescopic rods are respectively fixedly connected to the connecting frame and the movable frame.

2. The aquaculture experimental pond for water quality monitoring using zebrafish as claimed in claim 1, characterized in that: The adjustment component also includes a support spring, and two ends of the support spring are fixedly connected to the connecting frame and the movable frame respectively.

3. The aquaculture experimental pond for water quality monitoring using zebrafish as claimed in claim 2, characterized in that: The driving member includes a support seat and a first motor, the support seat is fixedly connected to the connecting frame and is located above the connecting frame, the first motor is fixedly connected to the support seat and is located inside the support seat, and one end of the connecting rod is fixedly connected to the output end of the first motor.

4. The aquaculture experimental pond for water quality monitoring using zebrafish as claimed in claim 3, characterized in that: The telescopic rod comprises an inner rod and an outer tube, the outer tube is fixedly connected to the connecting frame and is located above the connecting frame, one end of the inner rod is slidably connected to the outer tube, and the other end of the inner rod is fixedly connected to the movable frame.

5. The aquaculture experimental pond for water quality monitoring using zebrafish as claimed in claim 4, characterized in that: The transverse movement module includes a mounting frame, a screw rod, a screw rod nut and a second motor. The mounting frame is fixedly connected to the pool body and is located on the outer side wall of the pool body. Both ends of the screw rod are rotatably connected to the mounting frame. The screw rod nut is sleeved on the outside of the screw rod and cooperates with the screw rod thread. The screw rod nut is also movably connected to the mounting frame and is located in the mounting frame. The second motor is arranged on the outer side wall of the mounting frame. The output end of the second motor is connected to the screw rod. The connecting frame is fixedly connected to the screw rod nut and is located above the screw rod nut.

6. The aquaculture experimental pond for water quality monitoring using zebrafish as claimed in claim 1, characterized in that: The aquaculture experimental pond for monitoring water quality using zebrafish also includes a handle, which is fixedly connected to the camera and is located on the outer side wall of the camera.

7. A method for monitoring water quality using zebrafish, applied to the aquaculture experimental pond for monitoring water quality using zebrafish as claimed in claim 5, characterized in that: The steps include: conveying the monitored water body in the tank body; The water inlet pipe and the water outlet pipe are used in conjunction with a solenoid valve to simulate the flow and changes of natural water bodies; Zebrafish were placed in the tank; The camera is installed above the pool body, and cooperates with a timer to regularly record the behavior changes of zebrafish; Record water quality monitoring data and zebrafish behavior observations and generate detailed data reports.

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