Preparation method of semen cassiae zymolyte as well as product and application of semen cassiae zymolyte

Abstract

The invention discloses a preparation method of semen cassiae zymolyte and a product and application thereof, and the preparation method comprises the following steps: mixing semen cassiae, an enzyme preparation and drinking water, carrying out heat preservation reaction, and then carrying out enzyme deactivation, solid-liquid separation and drying to obtain zymolyte; wherein the enzyme preparation comprises hydrolase and / or transferase, the proportion of the cassia seed zymolyte and the aurantio-obtusin in the total anthraquinone is larger than or equal to 50%, and the transfer rate of the total anthraquinone is larger than or equal to 80%. According to the method, any one or more of cyclodextrin glucoside transferase, amylase, lipase or cellulase is used, the enzyme is used for solving the skyrmion, and the prepared zymolyte, namely the aurantio-obtusin accounts for more than or equal to 50%; the total anthraquinone transfer rate is greater than or equal to 80%. According to the invention, hydrolase and / or transferase and enzyme are used for solving the skyrmion, zymolyte is obtained, and the method can also be applied to soft sweets, solid preparations, soft capsules, hard capsules and lipidosome.

Description

Technical Field

[0001] The invention belongs to the technical field of enzymolysis product extraction, and specifically relates to a preparation method of cassia seed enzymolysis product and its product and application. Background Art

[0002] The Chinese Pharmacopoeia stipulates the anthraquinone components of Cassia seed. The raw product contains chrysophanol (C 15 H 10 O4) shall not be less than 0.20%, containing cassia bark (C 17 H 14 O7) shall not be less than 0.080%; after processing, it contains chrysophanol (C 15 H 10 O4) shall not be less than 0.12%, containing cassia bark (C 17 H 14 O7) shall not be less than 0.080%; the proportion of chrysophanol is less than 50%. Zhang Yi et al. determined the Cassia seeds from different origins by HPLC. The samples from each origin showed that the five anthraquinone components were mainly chrysophanol, and chrysophanol accounted for 14.9% to 32.21%. The traditional process uses organic solvents such as ethyl acetate, chloroform and other directional extraction and column separation to obtain a high proportion of chrysophanol.

[0003] The total sugar content in Cassia seed is 30.6%, and starch is the main component. The water extract is difficult to filter and dry. The crude protein content in Cassia seed is 19.09%, and the oil content is 4.97%, which is mainly unsaturated fatty acids, accounting for 74%. The extract is difficult to dry with organic solvents, and excipients need to be added to assist drying, but the corresponding content of the finished product will be reduced. The industry also uses activated carbon, diatomaceous earth and other adsorption aids, but there are losses, with a loss rate of about 20-50%;

[0004] Anthraquinones mainly include chrysophanol, rhamnolide, rhamnolide methyl ether, aloe-rhamnolide, etc. Most of them contain phenolic hydroxyl groups, which will be freed and precipitated after acidification; free anthraquinone components are generally soluble in organic solvents such as ethanol, ether, benzene, chloroform, etc., and are basically insoluble in water. The industry can improve clarity through membrane filtration and other methods, but the anthraquinone components will lose 20-30%, or even higher. And it is difficult to apply to aqueous solution beverage systems after acid-heat treatment.

[0005] CN101967090A discloses a process for extracting cassia bark extract, which comprises crushing selected cassia seeds into coarse powder, extracting in solvent gasoline, discarding the extract, adding an alcohol solvent to the residue for ultrasonic extraction, concentrating the extract, and extracting with ethyl acetate by Soxhlet reflux, concentrating the ethyl acetate extract and applying it to a polyamide chromatography column, eluting with petroleum ether-acetone and acetone-methanol, recovering the solvent from the eluate under low temperature and reduced pressure to obtain a crude product, and recrystallizing the crude product with N-dimethylformamide / acetone to obtain cassia bark extract. The invention aims to obtain high-purity cassia bark extract with a purity between 98.27% and 98.62%, but it does not pay attention to the proportion of cassia bark extract in the extract, and the invention uses an organic solvent, has a low yield, and takes a long extraction time, and is not suitable for industrial production. Summary of the invention

[0006] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0007] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0008] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing Cassia seed hydrolysate.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions: a method for preparing Cassia seed hydrolysate, comprising: mixing Cassia seed, enzyme preparation and drinking water, heat-preserving and reacting, inactivating the enzyme, separating the solid from the liquid, and drying to obtain the hydrolysate;

[0010] Wherein, the enzyme preparation comprises hydrolase and / or transferase;

[0011] In the cassia seed hydrolysate, the proportion of cassia truncatin in the total anthraquinones is ≥50%, and the total anthraquinone transfer rate is ≥80%.

[0012] As a preferred embodiment of the preparation method of the present invention, the hydrolase is selected from one or more of 4-α-glycosyltransferase, α-amylase, β-amylase, glucoamylase, α-galactosidase, β-galactosidase, cellulase, pectinase and lipase.

[0013] As a preferred embodiment of the preparation method of the present invention, wherein:

[0014] The transferase is selected from one or more of fructosyltransferase, cyclodextrin glucosyltransferase, transglucosidase and galactosyltransferase.

[0015] As a preferred embodiment of the preparation method of the present invention, the Cassia seed further comprises a pre-treatment of crushing, and / or grinding, and / or water swelling.

[0016] As a preferred embodiment of the preparation method of the present invention, the mass ratio of Cassia seed to complex enzyme preparation is 100:0.1-10.

[0017] As a preferred embodiment of the preparation method of the present invention, the hydrolase is selected from 4-α-glycosyltransferase, lipase and cellulase;

[0018] The transferase is selected from cyclodextrin glucosyltransferase.

[0019] As a preferred embodiment of the preparation method of the present invention, the mass ratio of the enzyme preparation, wherein the mass ratio of cyclodextrin glucoside transferase, 4-α-glycosyl transferase, lipase and cellulase is 0.01-1:0.1-1:0.1-1:0.1-1.

[0020] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing a Cassia seed hydrolysate, wherein in the Cassia seed hydrolysate, nerine cassiae accounts for ≥50% of the total anthraquinones and the total anthraquinone transfer rate is ≥80%.

[0021] Another object of the present invention is to overcome the deficiencies in the prior art and provide an application of Cassia seed hydrolysate in the preparation of beverages, gel candies, soft capsules, hard capsules, solid preparations, and liposomes.

[0022] Beneficial effects of the present invention:

[0023] (1) The present invention uses hydrolase and / or transferase to enzymatically hydrolyze Cassia seed, and the enzymatic hydrolyzate Cassiae sibiricum accounts for ≥50% of the total anthraquinones, and the total anthraquinone transfer rate is ≥80%, which can increase the total anthraquinone content of Cassia seed by at least 10%.

[0024] (2) The present invention uses hydrolases and / or transferases to enzymatically hydrolyze the seeds to obtain enzymatic hydrolysates, which are applied to liquid preparations and are easy to disperse and have good acid and heat stability.

[0025] (3) The present invention uses hydrolases and / or transferases to enzymatically hydrolyze the seeds to obtain enzymolyzates, which can also be applied to soft candies, solid preparations, soft capsules, hard capsules, and liposomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0027] Figure 1 It is the liquid chromatogram of commercially available Cassia seed powder 1 in Comparative Example 1 of the present invention.

[0028] Figure 2 It is the liquid chromatogram of commercially available Cassia seed powder 2 in Comparative Example 1 of the present invention.

[0029] Figure 3 It is the liquid chromatogram of the enzymatic hydrolysate prepared in Example 1 of the present invention.

[0030] Figure 4 It is the liquid chromatogram of the enzymatic hydrolysate prepared in Example 5 of the present invention.

[0031] Figure 5 This is a comparison chart of the clarification of the enzymatic hydrolysate in Comparative Example 2 of the present invention and the commercially available material. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0033] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0035] The total anthraquinone detection method of the present invention is:

[0036] Reference substance:

[0037] Cassia aurantium: CAS number: 67979-25-3;

[0038] Aloe-emodin: CAS No.: 481-72-1;

[0039] Rhein: CAS No.: 478-43-3;

[0040] Rhein: CAS No.: 518-82-1;

[0041] Chrysophanol: CAS No.: 481-74-3;

[0042] Physcion: CAS No.: 521-61-9;

[0043] Anthraquinone standard stock solution: Accurately weigh about 10 mg of each of auranthus cassiae, aloe-emodin, rhein, rhein, chrysophanol, and physophanol methyl ether into a 100 mL volumetric flask, add an appropriate amount of methanol and ultrasonically dissolve, cool, dilute to the scale, shake well, and make the concentration about 100 μg / mL

[0044] Preparation of anthraquinone standard series solutions: Accurately pipette 0.10mL, 0.50mL, 1.00mL, 2.00mL, and 5.00mL of anthraquinone standard solution into a 10mL brown volumetric flask, dilute to the mark with methanol, mix thoroughly, and prepare a standard curve. The concentrations of this standard series working solution are 1.00μg / mL, 5.00μg / mL, 10.00μg / mL, 20.00μg / mL, and 50.00μg / mL, respectively. Use the peak area of ​​the standard solution as the ordinate and the concentration as the abscissa to draw a standard curve.

[0045] Sample determination: Weigh the mixed sample (accurate to 0.0001g) in a 25mL volumetric flask, add about 10mL of methanol, ultrasonically extract for 30min, cool to room temperature, and dilute to the mark with methanol. Filter, accurately pipette 5mL of supernatant into the sample tube, and evaporate the solvent in a 60℃ water bath with nitrogen. Add 10mL of hydrochloric acid solution and hydrolyze at 100℃ for 1h. Transfer the hydrolyzate to a 150mL separatory funnel, rinse the sample tube with a small amount of water, add the rinse solution to the separatory funnel, add chloroform to extract until the lower solution is colorless, 20mL each time, combine the lower organic phase, concentrate to dryness, add methanol to dissolve and dilute to 20mL. Filter through a 0.45μm filter membrane into a liquid phase injection bottle for testing.

[0046] Chromatographic column: C18 reverse phase column, mobile phase: acetonitrile: phosphoric acid solution, injection volume: 5 μL, detection wavelength: 430 nm;

[0047] Calculation method: X = (ρ*V*F) / (M*1000)*100%

[0048] X- content of certain anthraquinone, %

[0049] ρ-Concentration of each anthraquinone obtained according to the standard curve, mg / L

[0050] M-sampling amount of the sample, mg

[0051] V- constant volume of sample solution, mL

[0052] F- dilution factor of sample solution.

[0053] Total anthraquinone content, % = X (auranthus cassiae + aloe-emodin + rhein + rhein + chrysophanol + physophanol methyl ether)

[0054] Auricularia cassiae ratio, % = (Auricularia cassiae content, %) / (Anthraquinone content, %) * 100

[0055] Transfer rate % = (total anthraquinone mass of Cassia seed hydrolysate, g) / (total anthraquinone mass of Cassia seed raw material, g) × 100%.

[0056] The enzymes involved in the embodiments of the present invention are:

[0057] Cyclodextrin glucoside transferase (derived from Bacillus licheniformis, with an enzyme activity of 200 U / mL), 4-α-glycosyltransferase (derived from Bacillus pallidum, with an enzyme activity of 100 U / mL), lipase (derived from Aspergillus niger, with an enzyme activity of 9000 PLU / g), and cellulase (derived from Aspergillus niger, with an enzyme activity of 700 EGU / mL) were purchased from Novozymes.

[0058] Example 1

[0059] 40 kg of Cassia seed, 160 g of cyclodextrin glucosyltransferase, 80 g of 4-α-glycosyltransferase, 80 g of lipase, 160 g of cellulase, 800 kg of purified water, keep warm at 55 ° C for 3 h, heat to 85 ° C, and inactivate the enzyme for 30 min;

[0060] 2000r / min, centrifuge to remove residue, and concentrate the centrifuge to a sugar content of 30°Bx;

[0061] Spraying is performed under the conditions of an inlet air temperature of 170° C. and an outlet air temperature of 70° C. to obtain the Cassia seed hydrolysate of the present invention;

[0062] After testing and calculation, it was found that auranthus cassiae accounted for 79.92% of the total anthraquinones; the total anthraquinone transfer rate was 88.5%.

[0063] Example 2

[0064] 900g of Cassia seed, add 0.5g of cyclodextrin glucosyltransferase, 0.1g of 4-α-glycosyltransferase, 0.1g of lipase, 0.2g of cellulase, add 1.8L of tap water, stir evenly, keep warm at 40℃ for 2h, and heat to 95℃ for 10min to inactivate the enzyme;

[0065] The residue is removed by centrifugal filtration, the filtrate is collected, concentrated to a sugar content of 50° Bx, and vacuum dried at low temperature to obtain the Cassia seed hydrolysate of the present invention;

[0066] After testing and calculation, it was found that auranthus cassiae accounted for 53.8% of the total anthraquinones; the total anthraquinone transfer rate was 92.3%.

[0067] Example 3

[0068] 500 kg of Cassia seed, 1.22 kg of cyclodextrin glucosyltransferase, 24.39 kg of 4-α-glycosyltransferase, 24.39 kg of cellulase, 10,000 kg of tap water, stir evenly, keep warm at 40°C for 2 hours, heat to 95°C for 10 minutes to inactivate the enzymes;

[0069] Centrifugal filtration to remove filter residue;

[0070] The filtrate was collected, concentrated to a sugar content of 50°Bx, and vacuum dried to obtain Cassia seed hydrolysate;

[0071] After testing and calculation, cassia bark extract accounts for 92.0% of the total anthraquinones; the total anthraquinone transfer rate is 99.3%.

[0072] Example 4

[0073] 200g of Cassia seed, 5g of cyclodextrin glucosyltransferase, 5g of lipase, 2L of tap water, stir evenly, keep stirring at 50℃ for 2h, and heat to 95℃ for 10min to inactivate the enzyme;

[0074] Centrifugal filtration to remove filter residue;

[0075] The filtrate is collected, concentrated and dried in vacuo to obtain the Cassia seed hydrolysate of the present invention;

[0076] After testing and calculation, it was found that auranthus cassiae accounted for 53.1% of the total anthraquinones; the total anthraquinone transfer rate was 95.2%.

[0077] Example 5

[0078] 200g of Cassia seed, 0.12g of cyclodextrin glucosyltransferase, 0.12g of cellulase, 2L of tap water, stir evenly, keep stirring at 55℃ for 5h, and heat to 95℃ for 10min to inactivate the enzyme;

[0079] Centrifugal filtration to remove filter residue;

[0080] The filtrate is collected, concentrated and dried in vacuo to obtain the Cassia seed hydrolysate of the present invention;

[0081] After testing and calculation, it was found that auranthus cassiae accounted for 62.1% of the total anthraquinones; the total anthraquinone transfer rate was 88.3%.

[0082] Example 6

[0083] 200g of Cassia seed, 12g of cyclodextrin glucosyltransferase, 8g of 4-α-glycosyltransferase, 2L of tap water, stir evenly, keep stirring at 60℃ for 2h, and heat to 95℃ for 10min to inactivate the enzyme;

[0084] Centrifugal filtration to remove filter residue;

[0085] The filtrate is collected, concentrated and dried in vacuo to obtain the Cassia seed hydrolysate of the present invention;

[0086] After testing and calculation, it was found that auranthus cassiae accounted for 71.8% of the total anthraquinones; the total anthraquinone transfer rate was 89.9%.

[0087] Example 7

[0088] 200g of Cassia seed, add 6g of cyclodextrin glucosyltransferase, add 2L of tap water, stir evenly, keep stirring at 65℃ for 5h, and heat to 95℃ for 10min to inactivate the enzyme;

[0089] Centrifugal filtration to remove filter residue;

[0090] The filtrate is collected, concentrated and dried in vacuo to obtain the Cassia seed hydrolysate of the present invention;

[0091] After testing and calculation, it was found that auranthus cassiae accounted for 53.8% of the total anthraquinones; the total anthraquinone transfer rate was 92.3%.

[0092] Comparative Example 1

[0093] Commercially available Cassia seed powder 1, its preparation process is as follows:

[0094] Take the Cassia seed aqueous solution and fill the resin column with raw material: D101 macroporous resin (m:m) = 0.5:1, according to the loading concentration of 15g / L and the loading flow rate of 2BV * / h, after the loading is completed, the resin is eluted with 3BV of water at a flow rate of 5BV / h, and the washing liquid is discarded; 3BV of 30% ethanol, 70% ethanol, and 95% ethanol aqueous solutions are used for gradient elution respectively, and the 70% and 95% ethanol eluate components are collected, and the ethanol is recovered by double-effect concentration. The concentrate is spray-dried at an inlet air temperature of 180-190°C and an outlet air temperature below 90°C to obtain the obtained product.

[0095] *Note 1: BV – column volume.

[0096] Comparative Example 2

[0097] Commercially available Cassia seed powder 2, the preparation process of which is as follows:

[0098] Take the Cassia seed aqueous solution, pass it through a 500-mesh filter bag centrifuge at a centrifugal speed of 2600 rpm / min, collect the centrifuge liquid, pass it through a 0.2um organic membrane made of polyvinylidene fluoride (PVDF), and after the filtration is completed, wash it with water for 2-3 membrane volumes, and collect the permeate and the top wash liquid;

[0099] The concentrate is prepared by spray drying at 55-65°C, -0.085Mpa and a double-effect concentration of 35-40% sugar content, with an inlet air temperature of 175°C and an outlet air temperature below 90°C.

[0100] Commercially available Cassia seed powder 1 (resin purification process), commercially available Cassia seed powder 2 (membrane treatment process), Cassia seed hydrolysate 1 (finished product of the present invention, Example 1), Cassia seed hydrolysate 2 (finished product of the present invention, Example 2) were acidified to pH 3 by adding citric acid at a concentration of 1%, boiled at 100°C for 1h, cooled to room temperature, and centrifuged at 8000r / min for 20min, as shown in Table 1 below.

[0101] Table 1 Comparison of enzymatic hydrolysates and commercially available materials

[0102] Compare Projects Commercially available 1 (resin process) Commercially available 2 (membrane treatment) Enzyme hydrolysate 1 Enzyme hydrolysate 2 Percentage of aurantium cassiae, % 1.82 39.5 79.92 53.8 Anthraquinone retention rate in supernatant, % 2.78 56.5 96.8 91.6 Clarifying pictures Figure 5 a Figure 5 b Figure 5 c Figure 5 d

[0103] Figure 1 Corresponding to the commercially available 1 in Table 1, resin process, auranthus cassiae in anthraquinone components (auranthus cassiae + aloe-emodin + rhein + emodin + chrysophanol + physophanol methyl ether), accounting for 1.82%;

[0104] Figure 2 Corresponding to the commercial product 2 in Table 1, the membrane filtration process, calculated by the liquid phase method, auranthus cassiae in the total anthraquinones (auranthus cassiae + aloe-emodin + rhein + emodin + chrysophanol + physophanol methyl ether), accounted for 39.5%;

[0105] Figure 3 The enzymolysis product 1 in the corresponding table 1 is the enzymolysis product of the present patent. According to the calculation by the liquid phase method, the content of auranthus cassiae in the total anthraquinones (auranthus cassiae + aloe-emodin + rhein + emodin + chrysophanol + physophanol methyl ether) is 79.92%;

[0106] Figure 4 The hydrolysate 2 in the corresponding Table 1 is the hydrolysate of the present patent. According to the calculation by the liquid phase method, auranthus cassiae accounts for 53.8% of the total anthraquinones (auranthus cassiae + aloe-emodin + rhein + emodin + chrysophanol + physophanol methyl ether).

[0107] Comparative Example 3

[0108] Under the conditions described in Example 1, different enzyme preparation combinations were designed, as shown in Table 2 below.

[0109] Table 2 Effects of different enzyme preparation combinations

[0110] Enzyme type Condition 1 Condition 2 Condition 3 Transglutaminase (TG) 0g 0g 160g Cyclodextrin glucosyltransferase 480g 0g 0g 4-α-Glycosyltransferase 0g 120g 80g Lipase 0g 120g 80g Cellulase 0g 240g 160g Percentage of aurantium cassiae, % 86.3％ 36.2％ 35.2％ Total anthraquinone transfer rate, % 52.7％ 8.74％ 8.7％

[0111] It can be seen that 4-α-glycosyltransferase, cellulase or lipase can release the anthraquinone components bound and restrained by dietary fiber or oil in Cassia seed through wall breaking and hydrolysis, and also hydrolyze polysaccharides such as macromolecular starch into small molecular oligosaccharides and monosaccharides such as glucose and dextrin.

[0112] Cyclodextrin glucoside transferase can utilize the dextrin components produced by enzymatic hydrolysis to promote the binding of anthraquinone components, especially cassia bark, with sugar groups, thereby increasing the hydrophilicity of anthraquinone components. There is synergy between cyclodextrin glucoside transferase and the combination of 4-α-glycosyltransferase, cellulase, and lipase. This method does not require the addition of additional dextrin excipients and can utilize the plant's own costs to achieve a cassia bark proportion of ≥50% and a total anthraquinone transfer rate of ≥80%, which is more green and economical.

[0113] Comparative Example 4

[0114] Recipe A:

[0115] Ingredient amount ( / 30mL): 200 mg of Cassia seed hydrolysate (prepared by the process of Example 1), 2500 mg of concentrated apple juice, 110 mg of citric acid, 20 mg of xanthan gum, 115 mg of food flavoring, and drinking water.

[0116] Recipe B:

[0117] Ingredient quantity ( / 30mL): concentrated prune juice 200mg, concentrated apple juice 2500mg, citric acid 110mg, xanthan gum 20mg, food flavoring 115mg, drinking water.

[0118] Recipe C:

[0119] Ingredient quantity ( / 30mL): concentrated prune juice 100mg, cassia seed hydrolysate 100mg, concentrated apple juice 2500mg, citric acid 110mg, xanthan gum 20mg, food flavoring 115mg, drinking water.

[0120] According to the above formula ratio, add the materials into a 100L dissolution tank respectively, add 80±5℃ hot water to keep warm and dissolve, pass through a 200-mesh filter, adjust the filtrate to about pH = 4 with citric acid, pass through a 400-mesh sieve, sterilize the filtrate at 121℃ for 15s at a flow rate of 20-30L / h, cool to about 90℃, and package in a sterile filling room at 30mL / bottle, and cool to below 40℃ with cold air.

[0121] Constipation is a symptom (group of symptoms) characterized by difficulty in defecation and / or decreased bowel movement frequency and hard and dry stool. Difficult defecation includes straining during defecation, difficulty in defecation, feeling of incomplete defecation, feeling of anorectal obstruction, time-consuming defecation and the need for assisted defecation. Decreased bowel movement frequency means less than 3 bowel movements per week. Chronic constipation lasts for at least 6 months.

[0122] The population was recruited according to the following scheme, and the ovulation-stimulating efficacy of the above-mentioned formula was tested.

[0123] Experimental model: With the approval of the ethics committee, a questionnaire survey was conducted to screen subjects with defecation needs or constipation.

[0124] Test method: The same group of subjects, blank period of 3 days, test period of 3 days;

[0125] Test format: Take 1 bottle (1 bottle 30mL) on an empty stomach from 8:00 to 8:30 a.m. every day, eat breakfast and clock in half an hour later + fill out a questionnaire (including taking photos of bowel movements);

[0126] Evaluation indicators: effective rate of defecation promotion (the number of people with increased total number of bowel movements during the test period compared with the blank period), stool morphology score, and number of bowel movements.

[0127] Effectiveness of defecation promotion % = the number of people with increased defecation frequency during the test period compared with the blank period / total number of people * 100;

[0128] Defecation frequency increase % = (average number of daily defecations during the 3-day test period - average number of daily defecations during the 3-day blank period) / average number of daily defecations during the 3-day blank period * 100 Table 3 Comparison of defecation-promoting effects of different formulas (n = 21)

[0129]

[0130]

[0131] It can be seen that the combination of Cassia seed hydrolysate and concentrated prune juice has a synergistic effect on promoting ovulation.

[0132] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the present invention.

Claims

1. A method for preparing a Cassia seed hydrolysate, characterized in that: include, The cassia seed, enzyme preparation and drinking water are mixed, and after heat preservation and reaction, the enzyme is inactivated, the solid-liquid is separated and dried to obtain the enzymolysis product; Wherein, the enzyme preparation comprises hydrolase and / or transferase, In the cassia seed hydrolysate, the proportion of cassia truncatin in the total anthraquinones is ≥50%, and the total anthraquinone transfer rate is ≥80%.

2. The preparation method according to claim 1, characterized in that: The hydrolase is selected from one or more of 4-α-glycosyltransferase, α-amylase, β-amylase, glucoamylase, α-galactosidase, β-galactosidase, cellulase, pectinase and lipase.

3. The preparation method according to claim 1 or 2, characterized in that: The transferase is selected from one or more of fructosyltransferase, cyclodextrin glucosyltransferase, transglucosidase and galactosyltransferase.

4. The preparation method according to claim 3, characterized in that: The pretreatment of the Cassia seed comprises the steps of crushing and / or grinding, and / or water swelling.

5. The preparation method according to any one of claims 1, 2 or 4, characterized in that: The mass ratio of cassia seed to enzyme preparation is 100:0.1-10.

6. The preparation method as claimed in claim 5, characterized in that: The hydrolase is selected from 4-α-glycosyltransferase, lipase and cellulase; the transferase is selected from cyclodextrin glucosyltransferase; The mass ratio of the cyclodextrin glucosyltransferase, 4-α-glycosyltransferase, lipase and cellulase is 0.01-1:0.1-1:0.1-1:0.1-1.

7. The Cassia seed hydrolysate obtained by the preparation method according to any one of claims 1 to 6, characterized in that: In the cassia seed hydrolysate, the proportion of cassia truncatin in the total anthraquinones is ≥50%, and the total anthraquinone transfer rate is ≥80%.

8. Use of the Cassia seed hydrolysate according to claim 7 in the preparation of beverages, gel candies, soft capsules, hard capsules, solid preparations, and liposomes.

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