Anti-wrinkle composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and discloses an anti-wrinkle composition as well as a preparation method and application thereof. The composition is prepared from the following components: melonic acid lactone, hydroxytyrosol, guaiazulene sodium sulfonate and betaine. The excellent oxidation resistance of hydroxytyrosol is utilized, and the hydroxytyrosol is combined with the melonic acid lactone capable of promoting cholesterol synthesis and the guaiazulene sodium sulfonate with an excellent anti-inflammatory effect, so that control is performed from multiple aspects of scavenging free radicals, synthesizing cholesterol to maintain skin elasticity and inhibiting release of inflammatory factors and inflammatory mediators; meanwhile, the betaine which is easy to permeate and has a calming effect is cooperated, and the four components are compounded, so that a synergistic effect can be achieved, and excellent anti-inflammatory, wrinkle-removing and anti-aging effects are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle composition and a preparation method and application thereof. Background Art

[0002] The skin is affected by many factors such as the external environment or its own metabolism, which will form free radicals. When free radicals destroy the collagen and active substances in normal cell membrane tissues, wrinkles are formed. As we age, metabolism slows down, water and subcutaneous fat decrease, elastic fibers gradually become thicker, and the skin becomes more wrinkled. Skin inflammation activates some enzymes present in the dermis, such as matrix metalloproteinases. These enzymes will break down the collagen fibers, hyaluronic acid, mucopolysaccharides, etc. in the dermis, resulting in enlarged pores, wrinkles, and sagging skin, which is usually called "inflammatory aging." Inflammation can also damage the skin's supporting tissues, elastin, and skin barrier function, thereby accelerating skin aging.

[0003] Inflammation is closely related to skin aging and is considered an important factor in skin aging. Currently, most of the anti-aging products on the market only work from the perspective of removing free radicals or supplementing or promoting collagen growth, but there are few studies on anti-aging from multiple aspects such as anti-inflammation. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides an anti-wrinkle composition and its preparation method and application, which can restore skin elasticity, promote fibroblast proliferation, reduce or eliminate free radicals in cells, inhibit the production of inflammatory cells and certain cytokines, reduce capillary permeability, and has significant anti-inflammatory, skin elasticity restoration, and skin firming effects.

[0005] The first aspect of the present invention provides a composition comprising the following components: mevalonolactone, hydroxytyrosol, sodium guaiacyl sulfonate and betaine.

[0006] The following is an introduction to the components and related mechanisms in the composition of the present invention:

[0007] Mevalonolactone is an ingredient discovered during the study of sake. It is extracted from the filtrate of the yeast Sacchromycopsis Fibuligera through biological culture. It is commonly found in many animals, plants and microorganisms in nature and participates in many biosynthetic processes. Mevalonolactone is extracted from "Saccharomyces cerevisiae" and is an optically 100% natural anti-aging ingredient. Mevalonolactone is hydrolyzed into mevalonic acid (mevalonic acid) in the skin. Mevalonic acid participates in cell metabolism pathways and promotes cholesterol synthesis. Cholesterol is important for maintaining the normal function of the skin. The synthesis amount decreases with age, resulting in insufficient cholesterol synthesis in the skin. Cholesterol is also one of the important components of skin cells, which helps maintain the elasticity and moisture content of the skin. Maintaining a moderate cholesterol level is also very important for maintaining the health and youthfulness of the skin.

[0008] Hydroxytyrosol is a natural polyphenol compound with strong antioxidant activity. It can also promote wound healing and eliminate free radicals. Hydroxytyrosol can promote the metabolism of skin cells, enhance skin elasticity and moisture, remove wrinkles and anti-aging. It also has anti-inflammatory effects and can reduce skin inflammation and redness.

[0009] Sodium guaiacyl sulfonate is an active ingredient extracted from Asteraceae plants (chamomile and chamomile). It has extremely excellent anti-inflammatory, anti-allergic and tissue repair effects. It is a substance with dual effects of targeted anti-inflammatory and tissue regeneration in the field of mucosal anti-inflammatory repair. It mainly plays the role of anti-allergy, anti-inflammatory, mucosal repair, and ulcer prevention. It should be noted that the use and dosage of sodium guaiacyl sulfonate should be determined according to the specific situation and cannot be used at will.

[0010] Betaine is an alkaloid with the chemical name N,N,N-trimethylglycine. It is mainly used as a moisturizer in cosmetics. This ingredient easily interacts with water to produce moisturizing ability. Betaine has excellent moisturizing ability and does not feel sticky when used. It can make the skin smoother after use, and it can also be used in combination with other ingredients to reduce skin allergies. It is an ideal calming moisturizer. In addition, it can balance the osmotic pressure of cells, and help cells restore balance and moisturizing state when the skin encounters a harsh environment, the osmotic pressure of cells is unbalanced, and the cells lose water.

[0011] Immunosenescence, cell senescence, and light exposure are the inducing factors of inflammatory aging. During the process of immunosenescence, immune cells such as T cells and macrophages will change, and the changes in macrophages are a target that skin care products can improve. Specifically, macrophages are usually divided into two polarized types: pro-inflammatory M1 and anti-inflammatory M2. The secretions of M1 macrophages will lead to an increase in the proportion of fibroblast aging, inhibit collagen synthesis, promote collagen decomposition, and significantly reduce collagen fibers. The number of fibroblasts in the human body gradually decreases, and the collagen and elastin secreted by them gradually decrease and break due to oxidation, and the supporting effect on the epidermis will disappear, causing uneven collapse, the dermis begins to thin, the skin elasticity deteriorates, and wrinkles begin to gradually appear. Therefore, inflammation plays an important role in skin aging. After many studies and experiments, the present invention selects four components, namely, mevalonolactone, hydroxytyrosol, sodium guaiacyl sulfonate and betaine, from numerous raw material components in the field. After compounding, the four components have a synergistic effect, play a role in multiple aspects such as scavenging free radicals, synthesizing cholesterol to maintain skin elasticity, and inhibiting the release of inflammatory factors and inflammatory mediators, and achieves excellent anti-inflammatory, wrinkle-removing and anti-aging effects.

[0012] In some embodiments of the present invention, the composition comprises the following components in parts by weight: 0.01-0.5 parts of mevalonolactone, 0.001-0.05 parts of hydroxytyrosol, 0.001-0.05 parts of sodium guaiacyl sulfonate and 0.1-5 parts of betaine.

[0013] In some embodiments of the present invention, the composition comprises the following components in parts by weight: 0.01-0.1 parts of mevalonolactone, 0.001-0.01 parts of hydroxytyrosol, 0.001-0.01 parts of sodium guaiacyl sulfonate and 0.1-1 parts of betaine.

[0014] In some embodiments of the present invention, the composition comprises the following components in parts by weight: 0.1-0.5 parts of mevalonolactone, 0.01-0.05 parts of hydroxytyrosol, 0.01-0.05 parts of sodium guaiacyl sulfonate and 1-5 parts of betaine.

[0015] The second aspect of the present invention provides a method for preparing the composition according to the first aspect of the present invention, comprising the following steps:

[0016] The components are mixed to obtain a composition.

[0017] The third aspect of the present invention provides an external skin preparation, wherein the external skin preparation comprises the composition according to the first aspect of the present invention.

[0018] In some embodiments of the present invention, the content of the composition in the external skin preparation is 0.1-6wt%, for example, it can be any point value among 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or a range value between two arbitrary point values.

[0019] A fourth aspect of the present invention provides a cosmetic comprising the composition according to the first aspect of the present invention.

[0020] In some embodiments of the present invention, the content of the composition in the cosmetic is 0.1-6wt%, for example, it can be any point value among 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or a range value between two arbitrary point values.

[0021] In some embodiments of the present invention, the cosmetic further comprises cosmetically acceptable excipients.

[0022] In some embodiments of the present invention, the auxiliary material includes at least one of a humectant, a thickener, a chelating agent, an emulsifier, an emollient, a pH adjuster, a preservative and a fragrance.

[0023] In some embodiments of the present invention, the cosmetic comprises any one of essence, lotion, cream or mask.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] The present invention utilizes the superior antioxidant property of hydroxytyrosol, combines mevalonolactone which can promote cholesterol synthesis, and sodium guaiacyl sulfonate which has excellent anti-inflammatory effect, controls multiple aspects from scavenging free radicals, synthesizing cholesterol to maintain skin elasticity, and inhibiting the release of inflammatory factors and inflammatory mediators, and simultaneously cooperates with betaine which is easily permeable and has a calming effect. By compounding the four components, a synergistic effect can be exerted, thereby achieving excellent anti-inflammatory, wrinkle-removing and anti-aging effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a comparison of wrinkles around the eyes before and after using the eye cream to which the composition of Example 10 is added. DETAILED DESCRIPTION

[0027] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following embodiments are listed for illustration. It should be pointed out that the following embodiments do not limit the protection scope of the present invention.

[0028] Unless otherwise specified, the raw materials, reagents or devices used in the present invention can be obtained from conventional commercial channels or by existing known methods.

[0029] The raw material information used in the embodiments of the present invention is as follows:

[0030] Mevalonolactone was obtained from Aidico Fine Chemicals (Zhejiang) Co., Ltd.;

[0031] Hydroxytyrosol was obtained from Fengyang Biological Research and Development (Nanjing) Co., Ltd.;

[0032] Sodium guaiacyl sulfonate was obtained from Shuobang (Huzhou) Biopharmaceutical Technology Co., Ltd.

[0033] Betaine is sourced from Nanjing Lizhihong Biotechnology Co., Ltd.

[0034] Examples 1-10

[0035] The compositions in Examples 1-10 use the components and their proportions in Table 1.

[0036] Table 1 Compositions and weight ratios of Examples 1-10

[0037]

[0038] The components were mixed according to the ratios in Table 1 to prepare the compositions in Examples 1-10, respectively.

[0039] Comparative Example 1

[0040] The composition in this example is different from that in Example 10 only in that this example does not contain mevalonolactone, and the remaining components and amounts are the same as those in Example 10.

[0041] Comparative Example 2

[0042] The composition in this example is different from that in Example 10 only in that hydroxytyrosol is not contained in this example, and the other components and amounts are the same as those in Example 10.

[0043] Comparative Example 3

[0044] The composition in this example is different from that in Example 10 only in that this example does not contain sodium guaiacyl sulfonate, and the remaining components and amounts are the same as those in Example 10.

[0045] Comparative Example 4

[0046] The composition in this example is different from that in Example 10 only in that betaine is not contained in this example, and the other components and amounts are the same as those in Example 10.

[0047] Comparative Example 5

[0048] The composition in this example is different from that in Example 10 only in that it does not contain mevalonolactone and hydroxytyrosol, and the remaining components and amounts are the same as those in Example 10.

[0049] Comparative Example 6

[0050] The composition in this example is different from that in Example 10 only in that this example does not contain mevalonolactone and sodium guaiacyl sulfonate, and the remaining components and amounts are the same as those in Example 10.

[0051] Comparative Example 7

[0052] The composition in this example is different from that in Example 10 only in that it does not contain mevalonolactone and betaine, and the remaining components and amounts are the same as those in Example 10.

[0053] Comparative Example 8

[0054] The composition in this example is different from that in Example 10 only in that hydroxytyrosol and sodium guaiacyl sulfonate are not contained in this example, and the remaining components and amounts are the same as those in Example 10.

[0055] Comparative Example 9

[0056] The composition in this example is different from that in Example 10 only in that hydroxytyrosol and betaine are not contained in this example, and the other components and amounts are the same as those in Example 10.

[0057] Comparative Example 10

[0058] The composition in this example is different from that in Example 10 only in that sodium guaiacyl sulfonate and betaine are not contained in this example, and the other components and amounts are the same as those in Example 10.

[0059] Comparative Example 11

[0060] The composition in this example is different from that in Example 10 only in that in this example, an equal amount of mevalonolactone is replaced with a filtrate of a bifid yeast fermentation product with similar efficacy, and the remaining components and amounts are the same as those in Example 10.

[0061] Comparative Example 12

[0062] The composition in this example is different from that in Example 10 only in that an equal amount of hydroxytyrosol is replaced with an olive fruit extract with similar efficacy, and the remaining components and amounts are the same as those in Example 10.

[0063] Comparative Example 13

[0064] The composition in this example is different from that in Example 10 only in that in this example, an equal amount of sodium guaiacyl sulfonate is replaced with a chamomile extract with similar efficacy, and the remaining components and amounts are the same as those in Example 10.

[0065] Comparative Example 14

[0066] The difference between the composition in this example and Example 10 is that in this example, an equal amount of betaine is replaced with trehalose with similar efficacy, and the other components and amounts are the same as those in Example 10.

[0067] Application Example 1

[0068] The compositions of Examples 1-10 and Comparative Examples 1-14 were added to the base formula of the essence in Table 2 to prepare essences No. 1-24, wherein the compositions of Examples 1-10 were used in essences No. 1-10; and the compositions of Comparative Examples 1-14 were used in essences No. 11-24. The addition amounts of the compositions of Examples 1-10 and Comparative Examples 1-14 are recorded in Table 3, based on the total mass percentage of the essence as 100%.

[0069] Application Example 2

[0070] The compositions of Examples 1-10 and Comparative Examples 1-14 were added to the eye cream base formula in Table 2 to prepare eye creams No. 1-24, wherein eye creams No. 1-10 respectively used the compositions of Examples 1-10; and eye creams No. 11-24 respectively used the compositions of Comparative Examples 1-14. Taking the total mass percentage of the eye cream as 100%, the addition amounts of the compositions of Examples 1-10 and Comparative Examples 1-14 are recorded in Table 3.

[0071] Application Example 3

[0072] The compositions of Examples 1-10 and Comparative Examples 1-14 were added to the basic formula of the facial mask liquid in Table 2 to prepare facial mask liquids No. 1-24, wherein the facial mask liquids No. 1-10 respectively used the compositions of Examples 1-10; and the facial mask liquids No. 11-24 respectively used the compositions of Comparative Examples 1-14. Taking the overall mass percentage of the facial mask liquid as 100%, the addition amounts of the compositions of Examples 1-10 and Comparative Examples 1-14 are recorded in Table 3.

[0073] Table 2 Basic formula of essence, eye cream and mask liquid

[0074]

[0075]

[0076] Table 3 Addition amount of the composition in Examples 1-10 and Comparative Examples 1-14

[0077]

[0078]

[0079] Product performance testing

[0080] (1) Anti-inflammatory evaluation test

[0081] 90 volunteers with sensitive skin aged 20-50 were selected and randomly divided into 9 groups, 10 in each group, and after washing their faces in the morning and evening every day, the essences added with the compositions of Examples 7-10 and Comparative Examples 3, 6, 8, 10, and 13 (the basic formula of the essences is shown in Table 2) were used for 4 consecutive weeks, once in the morning and once in the evening. The usage was statistically analyzed, and the results are shown in Table 4.

[0082] Anti-inflammatory effect efficacy criteria:

[0083] Cured: Skin redness, itching and pain symptoms completely disappeared;

[0084] Effective: Skin redness, itching and pain symptoms basically disappeared;

[0085] Ineffective: Skin redness, itching, and pain symptoms are the same as before or aggravated.

[0086] Table 4 Evaluation results of anti-inflammatory repair effect (number of people)

[0087]

[0088]

[0089] As can be seen from the results of Table 4, the essence containing the composition of Examples 7-10 of the present invention has good anti-inflammatory and repair effects, among which a rule can be found: within a certain concentration range, the higher the content of sodium guaiacyl sulfonate, the better the anti-inflammatory and repair effects. Through comparative experiments, it was found that the effect was significantly reduced by removing sodium guaiacyl sulfonate from the composition or replacing sodium guaiacyl sulfonate with other components; removing sodium guaiacyl sulfonate and any one of the components in the composition, the effect almost disappeared. Therefore, it can be inferred that sodium guaiacyl sulfonate is compounded with hydroxytyrosol, betaine, and mevalonolactone components, and the best anti-inflammatory effect is achieved through synergistic action.

[0090] (2) Skin wrinkle area test

[0091] 24 volunteers were selected, and the eye creams with the compositions of Examples 1-10 and Comparative Examples 1-14 were used on the left face, and the eye cream of the blank control group was used on the right face (the eye cream of the blank control group was prepared according to the basic formula of the eye cream in Table 2, without adding the compositions of the examples or comparative examples). The subjects used it once a day for 4 weeks according to the product use method; the use effect of the product was analyzed, and it was expressed by the skin wrinkle area reduction rate. The larger the skin wrinkle area reduction rate value, the better the effect.

[0092] Skin wrinkle area reduction rate = (total number of wrinkles in the designated area before testing - total number of wrinkles in the designated area after testing) / total number of wrinkles in the designated area before testing × 100%. The test results calculated according to this formula are shown in Table 5.

[0093] The comparison of wrinkles around the eyes before and after using the eye cream containing the composition of Example 10 for 28 days is shown in the attached figure. Figure 1 .

[0094] Table 5 Skin wrinkle area reduction rate

[0095]

[0096]

[0097] It can be seen from Table 5 above that after 4 weeks of using the eye cream added with the composition in the embodiment, the reduction rate of wrinkle area around the eyes was 7.8-18.2%, which is significantly higher than the reduction rate of wrinkle area around the eyes in the blank control group and the eye cream added with the composition in the comparative example (1.2-6.5%) after 4 weeks. By comparing Examples 1-3, 3-5, 5-7, and 7-9, it can be found that within the range of addition used by the present invention, the content of a certain component of the composition is reduced, and the wrinkle removal effect will also be reduced; it can be found from Comparative Examples 1-14 that the anti-wrinkle effect will be significantly reduced by removing any one or more components in the composition, or replacing the components in the composition with other components; the blank control group without the composition has almost no change. From the attached Figure 1 It can be found that after using the eye cream containing the composition of Example 10 for 28 days, the number of wrinkles around the eyes was significantly reduced using the VISIA facial analyzer.

[0098] (3) Skin elasticity test

[0099] 75 volunteers were selected, each in a group of three, and the facial mask liquids added with Examples 1-10 and Comparative Examples 1-14 were used in combination with ordinary fiber membrane cloth as facial masks, and were tried 3 times a week, using a mask for 15-20 minutes each time; the facial mask liquid in the blank control group was prepared according to the basic formula of the facial mask liquid in Table 2, without adding the composition in the examples or comparative examples. The skin of the cheekbones and corners of the eyes was taken as the test site. According to the group standard T / ZHCA 005-2019 "Test Method for the Effect of Cosmetics on Skin Elasticity", the skin elasticity characterization value R was measured before and 28 days after the trial. The greater the R value change rate (ie V), the better the improvement in skin elasticity. The calculation formula of the characterization value R is: R=U1 / U2. In the formula, U1 is the rebound displacement (mm) of the skin after eliminating the negative pressure, and U2 is the maximum tensile displacement (mm) of the skin when the negative pressure is loaded. The change rate V = (R2-R1) / R1×100%; wherein R1 is the skin elasticity characterization value before the trial, and R2 is the skin elasticity characterization value after the trial. The specific test average values ​​of the skin elasticity change rate of each group are shown in Table 6.

[0100] Table 6 Skin elasticity change rate

[0101]

[0102] As shown in Table 6 above, after 28 days of using the mask liquid added with the composition of the embodiment of the present invention, the skin elasticity change rate is 12.52-18.55%, which is significantly higher than the skin elasticity change rate (2.15-9.20%) of the blank control group and the mask liquid added with the composition in the comparative example after 28 days, further indicating that the components of the composition of the present invention have a synergistic effect and can significantly increase skin elasticity. In addition, by comparing the mask liquids added with Examples 1-3, 3-5, 5-7, and 7-9, it can be seen that within the range of addition used by the present invention, increasing the content of a certain component of the composition can further increase the skin elasticity change rate after using the mask liquid.

[0103] In summary, the mevalonolactone in the composition of the present invention helps to maintain the elasticity and moisture content of the skin; hydroxytyrosol is a natural polyphenol compound with strong antioxidant activity, can also promote wound healing, eliminate free radicals, remove wrinkles and anti-aging, and also has anti-inflammatory effects, which can reduce skin inflammation and redness and swelling; sodium guaiazulene sulfonate has extremely excellent anti-inflammatory, anti-allergic and tissue repair effects; betaine has excellent moisturizing ability, can make the face smoother after use, and it can also be used to reduce skin allergies in combination with other components, and is an ideal sedative moisturizer. The present invention unexpectedly found through compatibility research that the compounding of mevalonolactone, hydroxytyrosol, sodium guaiazulene sulfonate and betaine has a synergistic effect, thereby achieving excellent anti-inflammatory, wrinkle-removing and anti-aging effects.

[0104] The preferred embodiments of the present invention are specifically described above, but the invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the invention. These equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A composition, characterized in that The composition comprises the following components: mevalonolactone, hydroxytyrosol, sodium guaiacyl sulfonate and betaine.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 0.01-0.5 parts of mevalonolactone, 0.001-0.05 parts of hydroxytyrosol, 0.001-0.05 parts of sodium guaiacyl sulfonate and 0.1-5 parts of betaine.

3. The composition according to claim 2, characterized in that The composition comprises the following components in parts by weight: 0.01-0.1 parts of mevalonolactone, 0.001-0.01 parts of hydroxytyrosol, 0.001-0.01 parts of sodium guaiacyl sulfonate and 0.1-1 parts of betaine.

4. The composition according to claim 2, characterized in that The composition comprises the following components in parts by weight: 0.1-0.5 parts of mevalonolactone, 0.01-0.05 parts of hydroxytyrosol, 0.01-0.05 parts of sodium guaiacyl sulfonate and 1-5 parts of betaine.

5. The method for preparing the composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: The components are mixed to obtain a composition.

6. A skin preparation for external use, characterized in that The external skin preparation comprises the composition according to any one of claims 1 to 4.

7. The external skin preparation according to claim 6, characterized in that The content of the composition is 0.1-6 wt %.

8. A cosmetic, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 4.

9. The cosmetic according to claim 8, characterized in that: The content of the composition is 0.1-6 wt %.

10. The cosmetic according to claim 8, characterized in that: The cosmetics include any one of essence, lotion, cream or facial mask.

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