A device for efficiently separating and purifying uterine decidual NK cells

Abstract

The present invention relates to the technical field of NK cells, and proposes a device for efficiently separating and purifying uterine decidual NK cells, comprising a fixed mesh frame, a centrifuge tube and a support plate, wherein a fixed side plate is welded and fixed on the top surface of the fixed mesh frame, a first limiting groove is provided on the fixed side plate, a second limiting groove is provided through the fixed side plate, a connecting plate is welded and fixed on the fixed side plate, the connecting plate is welded and fixed to one end of a first spring, the other end of the first spring is welded and fixed to one end of a baffle rod, the baffle rod is slidably connected to the connecting plate, a multifunctional protective component is installed on the fixed mesh frame, an efficient positioning component is installed on the top of the fixed mesh frame, and an integrated processing component is installed on the top of the fixed mesh frame. Through the above technical scheme, the problem that the NK cell separation and purification device in the prior art cannot conveniently and stably assist the staff to perform multiple dripping, centrifugation and aspiration processes is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of NK cells, and in particular to a device for efficiently separating and purifying uterine decidual NK cells. Background Art

[0002] Decidual NK cells refer to a specific type of natural killer cells present in the decidua during pregnancy. They play a key role in supporting embryo implantation, promoting placental development and regulating maternal immunity. In order to study the function, characteristics and role of decidual NK cells in pregnancy, it is necessary to isolate and purify decidual NK cells to facilitate subsequent research and experiments.

[0003] The NK cell separation and purification process is cumbersome and requires multiple dripping, centrifugation and aspiration processes. However, the existing NK cell separation and purification devices cannot conveniently and stably assist the staff in performing multiple dripping, centrifugation and aspiration processes during actual work, and are of poor practicality, resulting in low work efficiency. Moreover, the existing NK cell separation and purification devices cannot conveniently and stably adsorb and fix the entire device on the work surface during work. Therefore, during operation, the staff may easily accidentally touch the device, causing the entire device to shake, which may lead to operational errors. After the use of the device, it cannot perform convenient and stable sealing and protection treatment on itself, thereby failing to ensure the convenience and stability of subsequent transportation work, and has poor safety. Moreover, after the work is completed, the existing NK cell separation and purification devices cannot conveniently and stably disassemble and install the used parts, thereby failing to ensure the convenience of subsequent cleaning of the device. Therefore, it is necessary to provide a device for efficiently separating and purifying uterine decidual NK cells to meet the needs of users. Summary of the invention

[0004] The present invention proposes a device for efficiently separating and purifying uterine decidual NK cells, which solves the problems that the NK cell separation and purification devices in the related art cannot conveniently and stably assist the staff to perform multiple dripping, centrifugation and aspiration processes, cannot conveniently and stably adsorb and fix the entire device on the work surface, cannot conveniently and stably perform sealing and protection treatment on themselves, and cannot conveniently and stably disassemble and install the used parts.

[0005] The technical solution of the present invention is as follows:

[0006] A device for efficiently separating and purifying uterine decidual NK cells comprises a fixed mesh frame, a centrifuge tube and a support plate, wherein a fixed side plate is welded and fixed on the top surface of the fixed mesh frame, a first limiting groove is provided on the fixed side plate, a second limiting groove is penetrated through the fixed side plate, a connecting plate is welded and fixed to one end of a first spring, the other end of the first spring is welded and fixed to one end of a baffle rod, the baffle rod is penetrated and slidably connected to the connecting plate, a multifunctional protective component is installed on the fixed mesh frame, an efficient positioning component is installed on the top of the fixed mesh frame, a partition is fixedly connected in the centrifuge tube, an integrated processing component is installed on the top of the fixed mesh frame, a magnetic immune sorter is installed and fixed on the top surface of the support plate, threaded holes are penetrated through the tops of the support plate and the fixed mesh frame, and a third threaded rod is threadedly connected in the threaded hole.

[0007] As a preferred solution of the present invention, wherein: the fixed side panels are symmetrically distributed on both sides of the top of the fixed net frame, the first limiting grooves are symmetrically distributed on both sides of the fixed side panels, the first limiting grooves correspond one-to-one with the second limiting grooves, the width of the second limiting grooves is equal to the width of the first limiting grooves, the connecting plates are symmetrically distributed on the fixed side panels, and the connecting plates correspond one-to-one with the blocking rods through the first springs.

[0008] As a preferred scheme of the present invention, wherein: the multifunctional protective component includes a protective top plate, a fixed handle is welded and fixed on the top surface of the protective top plate, a protective side plate is welded and fixed on the bottom surface of the protective top plate, the protective side plates are symmetrically distributed on both sides of the bottom of the protective top plate, a push plate is welded and fixed on the side surface of the protective side plate, the top surface of the push plate is fitted with the other end of the baffle rod, the protective side plate is slidably connected in the first limiting groove, the push plates are symmetrically distributed on both sides of the protective side plate, the push plate is slidably connected in the second limiting groove, the fixed handle is fixed to the top middle part of the protective top plate, and a first plug tube is welded and fixed to the fixed mesh frame.

[0009] As a preferred solution of the present invention, the inner top end surface of the first plug tube is welded and fixed to the top end of the second spring, the bottom end of the second spring is fixedly connected to the first rubber piston, the first rubber piston is slidably connected in the first plug tube, the top end surface of the first rubber piston is fixedly connected to the first plug rod, the first plug rod penetrates and is slidably connected in the first plug tube, the bottom end of the first plug tube is connected to a connecting tube, the connecting tube is welded and fixed in the fixed net frame, the bottom of the connecting tube is connected to a rubber suction cup, the top end surface of the first plug rod is fitted with the bottom end surface of the push plate, the push plate corresponds to the first plug tube one by one through the first plug rod, the second spring is sleeved on the first plug rod, the connecting tube is rectangular as a whole, and the rubber suction cups are equidistantly distributed at the bottom of the connecting tube.

[0010] As a preferred solution of the present invention, the high-efficiency positioning component includes a first servo motor, the first servo motor is welded and fixed in the fixed screen frame, a rotating plate is welded and fixed on the output shaft of the first servo motor, a rubber plate and a second plug tube are fixedly connected on the top surface of the rotating plate, a positioning plate is welded and fixed on the top of the second plug tube, a through slot is penetrated through the positioning plate, a first filter plate is welded and fixed on the top of the second plug tube, a first threaded rod is rotatably connected to the first filter plate, a threaded sleeve rod is threadedly connected to the first threaded rod, a second rubber piston is fixedly connected to the bottom end of the threaded sleeve rod, and the second rubber piston is slidably connected in the second plug tube, the output shaft of the first servo motor is fixed at the bottom center of the rotating plate, the rubber plate is fixed at the top center of the rotating plate, the second plug tube is symmetrically distributed on both sides of the rotating plate, the top surface of the second plug tube, the top surface of the first filter plate and the top surface of the positioning plate are flush, and the vertical center line of the positioning plate, the vertical center line of the through slot and the vertical center line of the rotating plate are located on the same vertical line.

[0011] As a preferred solution of the present invention, wherein: the bottom side end of the second plug tube is connected to the first air guide tube, the top end of the first air guide tube is connected to the second air guide tube, the second air guide tube is connected to the third plug tube, the third plug tube and the second air guide tube are both fixedly connected in the positioning plate, the third plug tube is slidably connected with a third rubber piston, the third rubber piston is fixedly connected with a second plug rod, the second plug rod penetrates and is slidably connected in the third plug tube, a clamping plate is welded and fixed to the end of the second plug rod, and a rubber pad is fixedly connected to the clamping plate.

[0012] As a preferred solution of the present invention, the second air guide tube is in an overall annular shape, the overall annular inner diameter of the second air guide tube is larger than the diameter of the through groove, four third plug tubes are provided, and the four third plug tubes are distributed at equal angles on the annular inner wall of the second air guide tube, the third plug tubes correspond one-to-one with the third rubber piston, and the third rubber piston corresponds one-to-one with the clamping plate through the second plug rod, the second plug rod is fixed to the middle part of the side end of the clamping plate, the cross-section of the clamping plate is in an arc shape, and the second plug tube and the third plug tube are both rectangular tubes.

[0013] As a preferred scheme of the present invention, wherein: the integrated processing component includes a hydraulic rod and a mounting plate, the bottom end of the hydraulic rod is installed and fixed on the fixed net frame, the top end of the hydraulic rod is fixedly connected with a connecting plate, a second servo motor is welded and fixed on the top surface of the connecting plate, a turntable and a first limit plate are welded and fixed on the output shaft of the second servo motor, a shift rod is welded and fixed on the turntable, a transmission shaft is rotatably connected on the bottom end surface of the connecting plate, a second limit plate is welded and fixed on the transmission shaft, a guide groove is opened through the second limit plate, the hydraulic rods are symmetrically distributed on both sides of the connecting plate, the side end surface of the first limit plate is in contact with the side end surface of the second limit plate, four guide grooves are provided, and the four guide grooves are equiangularly distributed on the second limit plate.

[0014] As a preferred scheme of the present invention, wherein: a baffle is welded and fixed to the bottom end of the transmission shaft, a positioning rod is welded and fixed to the bottom end surface of the baffle, the positioning rod is welded and fixed to one end of the third spring, and the other end of the third spring is welded and fixed to a block, the block is limited and slidably connected to the positioning rod, a positioning groove and a mounting groove are penetrated through the mounting plate, the positioning rod is penetrated and slidably connected in the positioning groove, the length of the positioning rod is greater than the thickness of the mounting plate, the third spring is symmetrically distributed on both sides of the positioning rod, the third spring corresponds to the block one by one, the cross-section of the block is a right-angle trapezoid, four mounting grooves are provided, and the four mounting grooves are equiangularly distributed on the mounting plate, and the first guide tube, the second guide tube, the third guide tube and the fourth guide tube are respectively slidably connected in the four mounting grooves, and the mounting plate is in a "cross" shape as a whole, and the positioning groove is opened at the center of the mounting plate.

[0015] As a preferred solution of the present invention, the side ends of the first flow guide tube, the second flow guide tube, the third flow guide tube and the fourth flow guide tube are all provided with a card slot, the mounting plate is welded and fixed to one end of a fourth spring, a card rod is welded and fixed to the other end of the fourth spring, the card rod is slidably connected in the mounting plate, the end of the card rod is snap-connected in the card slot, the card slot corresponds to the card rod one-to-one, a second filter plate is welded and fixed to the top of the third flow guide tube, a second threaded rod is threadedly connected to the second filter plate, a fourth rubber piston is rotatably connected to the bottom end of the second threaded rod, and the fourth rubber piston is slidably connected in the third flow guide tube.

[0016] The working principle and beneficial effects of the present invention are:

[0017] 1. The present invention is provided with a baffle rod and a multifunctional protective assembly. By utilizing the cooperation of the first spring and the baffle rod, the snap-fit ​​installation and disassembly separation of the protective side panels on the front and rear sides can be conveniently completed through the push plate. By utilizing the snap-fit ​​installation of the protective side panels on both sides, in cooperation with the protective top plate and the fixed side panels, the entire device can be conveniently and stably sealed and protected, while ensuring the convenience and stability of subsequent transportation of the device. When the device starts working, the protective side panels can be removed. At this time, under the elastic action of the second spring, the first rubber piston can be automatically driven to suck the air inside the connecting pipe. In cooperation with the internal negative pressure of each rubber suction cup, the entire device can be automatically and stably adsorbed and fixed on the workbench, thereby effectively preventing the staff from shaking the entire device during subsequent experimental operations, resulting in operational errors, which affects the stability and accuracy of NK cell separation and purification work; the practical stability and safety of the uterine decidual NK cell separation and purification device are increased.

[0018] 2. The present invention is provided with an efficient positioning component, which utilizes the threaded drive of the first threaded rod and the threaded sleeve rod to push the gas into each third plug tube through the movement of the second rubber piston, and utilizes the air pressure balance to drive the second plug rods on each third rubber piston to move toward the middle at the same time, and cooperates with the corresponding clamping plate to conveniently and stably clamp and fix the centrifuge tube, and can clamp and fix the centrifuge tube in the center, thereby ensuring the accuracy and stability of subsequent dripping operation and centrifugation of the centrifuge tube, and avoiding dripping errors that affect the subsequent separation effect; effectively improving the work efficiency of centrifuge tube positioning and fixation, and at the same time improving the accuracy and convenience of subsequent NK cell separation and purification work.

[0019] 3. The present invention is provided with an integrated processing assembly. The lever on the turntable and the guide grooves on the second limit plate cooperate to drive the mounting plate to rotate intermittently, and each rotation is 90°, thereby driving the first guide tube, the second guide tube, the third guide tube and the fourth guide tube to switch positions automatically and stably, thereby gradually assisting in the dripping and taking of the sample liquid, thereby conveniently completing the separation of NK cells; after the NK cell separation work is completed, the positioning rod and the stopper cooperate to conveniently complete the disassembly and replacement of the mounting plate, and by replacing the new mounting plate The plate and the new first guide tube, second guide tube, third guide tube and fourth guide tube can quickly carry out the subsequent dripping and liquid collection work of NK cells, so as to quickly carry out the purification treatment after separation, effectively improving the efficiency and convenience of uterine decidual NK cell separation and purification, and further improving the work efficiency of uterine decidual NK cell separation and purification; and by utilizing the cooperation of the card slot and the card rod, the first guide tube, the second guide tube, the third guide tube and the fourth guide tube can be conveniently and stably disassembled and installed after use, thereby ensuring the convenience of subsequent cleaning and reuse. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the connection structure between the fixed side plate and the first limiting groove of the present invention;

[0023] Figure 3 It is a schematic diagram of the connection structure of the protective top plate and the protective side plate of the present invention;

[0024] Figure 4 It is a schematic diagram of the connection structure between the baffle and the positioning rod of the present invention;

[0025] Figure 5 It is a schematic diagram of the overall main cross-sectional structure of the present invention;

[0026] Figure 6 The present invention Figure 5 The enlarged structural diagram at A in the middle;

[0027] Figure 7 The present invention Figure 5 The enlarged structural diagram at B in the middle;

[0028] Figure 8 It is a schematic diagram of the overall side view structure of the present invention;

[0029] Fig. 9 It is a schematic diagram of the top-sectional structure of the fixed side plate of the present invention;

[0030] Fig.10 This is a schematic diagram of the top-sectional structure of the connecting pipe of the present invention;

[0031] Fig.11 This is a schematic diagram of the main cross-sectional structure of the first plug tube of the present invention;

[0032] Fig.12 This is a schematic diagram of the main cross-sectional structure of the efficient positioning component of the present invention;

[0033] Fig.13 It is a schematic diagram of the main cross-sectional structure of the second air guide tube and the third plug tube of the present invention;

[0034] Fig.14 It is a schematic diagram of the top-sectional structure of the efficient positioning component of the present invention;

[0035] Fig.15 It is a bottom view structural diagram of the turntable, the first limiting plate and the second limiting plate of the present invention;

[0036] Fig.16 It is a schematic diagram of the top view of the mounting plate of the present invention;

[0037] Fig.17 It is a schematic diagram of the side view structure of the mounting plate of the present invention;

[0038] Fig.18 It is a schematic diagram of the side sectional structure of the third flow guide tube of the present invention.

[0039] In the figure: 1. Fixed screen frame; 2. Fixed side plate; 3. First limit slot; 4. Second limit slot; 5. Connecting plate; 6. First spring; 7. Stopper; 8. Multifunctional protection assembly; 801. Protective top plate; 802. Fixed handle; 803. Protective side plate; 804. Push plate; 805. First plug tube; 806. Second spring; 807. First rubber piston; 808. First plug rod; 809. Connecting tube; 810. Rubber suction cup; 9. Efficient positioning Components; 901, first servo motor; 902, rotating plate; 903, rubber plate; 904, second plug tube; 905, positioning plate; 906, through groove; 907, first filter plate; 908, first threaded rod; 909, threaded sleeve rod; 910, second rubber piston; 911, first air guide tube; 912, second air guide tube; 913, third plug tube; 914, third rubber piston; 915, second plug rod; 916, clamping plate; 917, rubber Rubber pad; 10, centrifuge tube; 11, partition; 12, integrated processing assembly; 1201, hydraulic rod; 1202, connecting plate; 1203, second servo motor; 1204, turntable; 1205, first limit plate; 1206, lever; 1207, transmission shaft; 1208, second limit plate; 1209, guide groove; 1210, baffle; 1211, positioning rod; 1212, third spring; 1213, stopper; 1214, mounting plate ; 1215, positioning groove; 1216, mounting groove; 1217, first guide tube; 1218, second guide tube; 1219, third guide tube; 1220, fourth guide tube; 1221, clamping groove; 1222, fourth spring; 1223, clamping rod; 1224, second filter plate; 1225, second threaded rod; 1226, fourth rubber piston; 13, support plate; 14, magnetic immune sorter; 15, threaded hole; 16, third threaded rod. DETAILED DESCRIPTION

[0040] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] like Figures 1 to 18As shown, this embodiment proposes a device for efficiently separating and purifying uterine decidual NK cells, including a fixed net frame 1, a centrifuge tube 10 and a support plate 13. A fixed side plate 2 is welded and fixed on the top surface of the fixed net frame 1, a first limiting groove 3 is opened on the fixed side plate 2, a second limiting groove 4 is penetrated and opened on the fixed side plate 2, a connecting plate 5 is welded and fixed on the fixed side plate 2, the connecting plate 5 is welded and fixed to one end of a first spring 6, the other end of the first spring 6 is welded and fixed to one end of a blocking rod 7, the blocking rod 7 is penetrated and slidably connected to the connecting plate 5, a multifunctional protective component 8 is installed on the fixed net frame 1, an efficient positioning component 9 is installed on the top of the fixed net frame 1, a partition 11 is fixedly connected in the centrifuge tube 10, an integrated processing component 12 is installed on the top of the fixed net frame 1, a magnetic immune sorter 14 is installed and fixed on the top surface of the support plate 13, a threaded hole 15 is penetrated and opened on the top of the support plate 13 and the fixed net frame 1, a third threaded rod 16 is threadedly connected to the threaded hole 15, and the cooperation of the blocking rod 7 and the multifunctional protective component 8 can be used to adjust the device as a whole. Convenient and stable sealing protection can ensure the convenience and stability of subsequent transportation of the device; and the device can automatically and stably adsorb and fix the entire device in the working state on the workbench, thereby effectively preventing the staff from shaking the entire device during subsequent experimental operations, resulting in operational errors, affecting the stability and accuracy of NK cell separation and purification; and in conjunction with the efficient positioning component 9, the centrifuge tube 10 can be clamped and fixed in the center to ensure the accuracy and stability of subsequent dripping and centrifugal operations of the centrifuge tube 10, avoiding dripping errors that affect subsequent separation effects; effectively improves the working efficiency of the positioning and fixation of the centrifuge tube 10, while improving the accuracy and convenience of subsequent separation and purification of NK cells, and the integrated processing component 12 can gradually assist in the dripping and collection of the sample liquid, thereby conveniently completing the separation and purification of NK cells, effectively improving the efficiency and convenience of the separation and purification of uterine decidual NK cells, and further improving the efficiency of the separation and purification of uterine decidual NK cells.

[0043] Example 2

[0044] like Figures 1 to 18 As shown, based on the same concept as the above-mentioned Example 1, this example also proposes a device for efficiently separating and purifying uterine decidual NK cells.

[0045] In this embodiment, the fixed side panels 2 are symmetrically distributed on both sides of the top of the fixed net frame 1, the first limiting grooves 3 are symmetrically distributed on both sides of the fixed side panels 2, the first limiting grooves 3 correspond one-to-one to the second limiting grooves 4, the width of the second limiting grooves 4 is equal to the width of the first limiting grooves 3, the connecting plates 5 are symmetrically distributed on the fixed side panels 2, the connecting plates 5 correspond one-to-one to the blocking rods 7 through the first springs 6, and by utilizing the cooperation of the first springs 6 and the blocking rods 7, the multifunctional protective assembly 8 can be conveniently engaged, installed, disassembled and separated through the push plate 804.

[0046] The multifunctional protective assembly 8 in this embodiment includes a protective top plate 801, a fixed handle 802 is welded and fixed on the top surface of the protective top plate 801, a protective side plate 803 is welded and fixed on the bottom surface of the protective top plate 801, the protective side plates 803 are symmetrically distributed on both sides of the bottom of the protective top plate 801, and push plates 804 are welded and fixed on the side end surfaces of the protective side plates 803, the top surface of the push plate 804 is in contact with the other end of the blocking rod 7, the protective side plates 803 are slidably connected in the first limiting groove 3, the push plates 804 are symmetrically distributed on both sides of the protective side plates 803, the push plates 804 are slidably connected in the second limiting groove 4, the fixed handle 802 is fixed in the middle part of the top of the protective top plate 801, and the first plug tube 805 is welded and fixed on the fixed net frame 1. By utilizing the snap-fit ​​installation of the protective side plates 803 on both sides, in cooperation with the protective top plate 801 and the fixed side plate 2, the device as a whole can be conveniently and stably sealed and protected, and at the same time, the convenience and stability of the subsequent transportation of the device can be ensured.

[0047] In this embodiment, the inner top surface of the first plug tube 805 is welded and fixed to the top of the second spring 806, the bottom end of the second spring 806 is fixedly connected to the first rubber piston 807, the first rubber piston 807 is slidably connected in the first plug tube 805, the top surface of the first rubber piston 807 is fixedly connected to the first plug rod 808, the first plug rod 808 penetrates and is slidably connected in the first plug tube 805, the bottom end of the first plug tube 805 is connected to the connecting tube 809, the connecting tube 809 is welded and fixed in the fixed net frame 1, the bottom of the connecting tube 809 is connected to the rubber suction cup 810, the top surface of the first plug rod 808 is in contact with the bottom surface of the push plate 804, and the push plate 804 is pushed through the first plug rod 808 corresponds to the first plug tube 805 one by one, the second spring 806 is sleeved on the first plug rod 808, the connecting tube 809 is rectangular as a whole, and the rubber suction cups 810 are equidistantly distributed at the bottom of the connecting tube 809. By removing the protective side plate 803, the first rubber piston 807 can be automatically driven to suck the air inside the connecting tube 809 under the elastic action of the second spring 806. The internal negative pressure of each rubber suction cup 810 can automatically and stably adsorb and fix the entire device on the workbench, thereby effectively preventing the staff from shaking the entire device during subsequent experimental operations, resulting in operational errors, affecting the stability and accuracy of NK cell separation and purification.

[0048] In this embodiment, the high-efficiency positioning component 9 includes a first servo motor 901, which is welded and fixed in the fixed net frame 1. A rotating plate 902 is welded and fixed on the output shaft of the first servo motor 901. A rubber plate 903 and a second plug tube 904 are fixedly connected to the top surface of the rotating plate 902. A positioning plate 905 is welded and fixed to the top of the second plug tube 904. A through groove 906 is penetrated through the positioning plate 905. A first filter plate 907 is welded and fixed to the top of the second plug tube 904. A first threaded rod 908 is rotatably connected to the first filter plate 907. A threaded sleeve rod 909 is threadedly connected to the first threaded rod 908. A second rubber piston 910 is fixedly connected to the bottom end of the threaded sleeve rod 909. The second rubber piston 910 slides The first servo motor 901 is connected to the second plug tube 904, the output shaft of the first servo motor 901 is fixed to the bottom center of the rotating plate 902, the rubber plate 903 is fixed to the top center of the rotating plate 902, the second plug tube 904 is symmetrically distributed on both sides of the rotating plate 902, the top surface of the second plug tube 904, the top surface of the first filter plate 907 and the top surface of the positioning plate 905 are flush, the vertical center line of the positioning plate 905, the vertical center line of the through groove 906 and the vertical center line of the rotating plate 902 are located on the same vertical line, the bottom side end of the second plug tube 904 is connected to the first air guide tube 911, the top end of the first air guide tube 911 is connected to the second air guide tube 912, the second air guide tube 912 is connected to the third plug tube 913, the third plug tube 913 and the second The air guide tubes 912 are all fixedly connected in the positioning plate 905, and a third rubber piston 914 is slidably connected in the third plug tube 913. A second plug rod 915 is fixedly connected to the third rubber piston 914. The second plug rod 915 penetrates and is slidably connected in the third plug tube 913. A clamping plate 916 is welded and fixed to the end of the second plug rod 915, and a rubber pad 917 is fixedly connected to the clamping plate 916. The second air guide tube 912 is annular as a whole, and the overall annular inner diameter of the second air guide tube 912 is greater than the diameter of the through groove 906. Four third plug tubes 913 are provided, and the four third plug tubes 913 are equiangularly distributed on the annular inner wall of the second air guide tube 912. The third plug tubes 913 correspond to the third rubber pistons 914 one by one, and the third rubber pistons 914 pass through the second The plug rod 915 corresponds to the clamping plate 916 one by one, and the second plug rod 915 is fixed at the middle part of the side end of the clamping plate 916. The cross section of the clamping plate 916 is arc-shaped. The second plug tube 904 and the third plug tube 913 are both rectangular tubes. The first threaded rod 908 and the threaded sleeve rod 909 are driven by threads, and the gas can be pushed into each third plug tube 913 through the movement of the second rubber piston 910. By using the air pressure balance, the second plug rods 915 on each third rubber piston 914 can be driven to move toward the middle at the same time. With the corresponding clamping plate 916, the centrifuge tube 10 can be conveniently and stably clamped and fixed. At the same time, the centrifuge tube 10 can be clamped and fixed at the center, ensuring the accuracy and stability of the subsequent dripping operation and centrifugal work of the centrifuge tube 10.Avoid dripping errors that affect subsequent separation results.

[0049] In this embodiment, the integrated processing component 12 includes a hydraulic rod 1201 and a mounting plate 1214. The bottom end of the hydraulic rod 1201 is fixedly mounted on the fixed net frame 1. The top end of the hydraulic rod 1201 is fixedly connected with a connecting plate 1202. A second servo motor 1203 is welded and fixed on the top surface of the connecting plate 1202. A turntable 1204 and a first limit plate 1205 are welded and fixed on the output shaft of the second servo motor 1203. A lever 1206 is welded and fixed on the turntable 1204. A transmission shaft 1207 is rotatably connected to the bottom end surface of the connecting plate 1202. A second limit plate 1208 is welded and fixed on the transmission shaft 1207. A guide groove 1209 is opened through the second limit plate 1208. The hydraulic rods 1201 are symmetrically distributed on On both sides of the connecting plate 1202, the side end surface of the first limiting plate 1205 is fitted with the side end surface of the second limiting plate 1208, and four guide grooves 1209 are arranged, and the four guide grooves 1209 are distributed at equal angles on the second limiting plate 1208. By utilizing the cooperation of the lever 1206 on the turntable 1204 and the guide grooves 1209 on the second limiting plate 1208, the mounting plate 1214 can be driven to rotate intermittently, and each rotation is 90°, thereby driving the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 to automatically and stably switch positions, thereby gradually assisting in the dripping and liquid collection of the sample liquid, and thus conveniently completing the separation of NK cells.

[0050] In this embodiment, a baffle 1210 is welded and fixed to the bottom end of the transmission shaft 1207, and a positioning rod 1211 is welded and fixed to the bottom end surface of the baffle 1210. The positioning rod 1211 is welded and fixed to one end of the third spring 1212, and a stopper 1213 is welded and fixed to the other end of the third spring 1212. The stopper 1213 is slidingly connected in the positioning rod 1211 with limited position. A positioning groove 1215 and a mounting groove 1216 are penetrated on the mounting plate 1214. The positioning rod 1211 is slidably connected in the positioning groove 1215. The length of the positioning rod 1211 is greater than the thickness of the mounting plate 1214. The third spring 1212 is symmetrically distributed on both sides of the positioning rod 1211. The third spring 1212 corresponds to the stopper 1213 one by one. The cross-section of the stopper 1213 is a right-angle trapezoid. Four mounting grooves 1216 are provided, and the four mounting grooves 1216 are equiangularly distributed on the mounting plate 1214. On the top, the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 are slidably connected in the four installation grooves 1216 respectively. The installation plate 1214 is in a "cross" shape as a whole. The positioning groove 1215 is opened in the central part of the installation plate 1214. After the NK cell separation work is completed, the cooperation of the positioning rod 1211 and the block 1213 can be used to conveniently complete the disassembly and replacement of the installation plate 1214. By replacing the new installation plate 1214 and the new first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220, the subsequent dripping and liquid collection of NK cells can be quickly carried out, so as to quickly carry out the purification treatment after separation, effectively improving the efficiency and convenience of the separation and purification of uterine decidual NK cells, and further improving the work efficiency of the separation and purification of uterine decidual NK cells.

[0051] In this embodiment, the side ends of the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 are all provided with a slot 1221, the mounting plate 1214 is welded and fixed to one end of the fourth spring 1222, and the other end of the fourth spring 1222 is welded and fixed to a clamping rod 1223, the clamping rod 1223 is slidably connected in the mounting plate 1214, the end of the clamping rod 1223 is clamped and connected in the slot 1221, the slot 1221 corresponds to the clamping rod 1223 one by one, and the top of the third guide tube 1219 is welded A second filter plate 1224 is fixed, and a second threaded rod 1225 is threadedly connected to the second filter plate 1224. The bottom end of the second threaded rod 1225 is rotatably connected to a fourth rubber piston 1226. The fourth rubber piston 1226 is slidably connected in the third guide tube 1219. By utilizing the cooperation of the card slot 1221 and the card rod 1223, the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 can be conveniently and stably disassembled and installed after use, thereby ensuring the convenience of subsequent cleaning and reuse.

[0052] It should be noted that the present invention is a device for efficiently separating and purifying uterine decidual NK cells. First, the staff can place the entire device on the work surface. At this time, each rubber suction cup 810 can fit tightly against the work surface. Then, the staff can use the thumb and index finger of both hands to pull the two baffle bars 7 on the fixed side panels 2 on the left and right sides and move them toward the middle at the same time. Under the movement of the two baffle bars 7, the push plates 804 on both sides of the protective side panels 803 can be moved away. At this time, the obstruction of the push plates 804 disappears, and under the elastic action of the second spring 806, the push plates 804 can be moved away. The first rubber piston 807 is driven to move upward, thereby driving the first plug rod 808 on the first rubber piston 807 to move upward. At this time, the first plug rod 808 can push the push plate 804 to move upward, completing the removal of the protective side plate 803. Subsequently, the staff can use the fixed handle 802 to pull the protective top plate 801 upward, thereby driving the protective side plate 803 to move out of the first limiting groove 3 on the fixed side plate 2, completing the separation of the protective side plate 803. Subsequently, the staff can conveniently place the removed and separated protective side plate 803 at other locations;

[0053] At the same time, under the upward movement of the first rubber piston 807, the air inside the connecting tube 809 can be sucked through the first plug tube 805, and then the air inside each rubber suction cup 810 can be sucked. At this time, negative pressure is formed inside each rubber suction cup 810. By utilizing the internal negative pressure of each rubber suction cup 810, the entire device can be automatically and stably adsorbed and fixed on the work surface, ensuring the stability and safety of the subsequent working state of the entire device;

[0054] Then the staff can insert the centrifuge tube 10 to be used into the through slot 906 on the positioning plate 905. The centrifuge tube 10 is SepTube TMDensity gradient centrifuge tube, at this time, the rotating plate 902 flexibly protects the bottom of the centrifuge tube 10 through the rubber plate 903, and then the staff can rotate the first threaded rod 908 on the first filter plate 907 on the top of the second plug tube 904 on both sides. Under the rotation of the first threaded rod 908, the threaded sleeve rod 909 connected by thread can push the second rubber piston 910 to move downward stably inside the rectangular second plug tube 904. Under the movement of the second rubber piston 910, the gas inside the second plug tube 904 can be pushed into the second gas guide tube 912 through the first air guide tube 911, and then the gas can be pushed to each third plug tube 913 At this time, under the action of air pressure, the gas in the third plug tube 913 can push the third rubber piston 914 to move outward automatically. Under the action of air pressure balance, the third rubber pistons 914 inside each third plug tube 913 move toward the center of the through groove 906 at the same time. Therefore, each third rubber piston 914 can drive the corresponding clamping plate 916 to move toward the middle at the same time through the second plug rod 915. Under the synchronous movement of each clamping plate 916, the rubber pad 917 can be used to clamp and fix the centrifuge tube 10 conveniently and stably, and at the same time, the centrifuge tube 10 can be clamped and fixed at the center, ensuring the accuracy and stability of the subsequent dripping operation and centrifugal work of the centrifuge tube 10.

[0055] Firstly, under the driving action of the second servo motor 1203, the turntable 1204 and the first limit plate 1205 on the output shaft can be driven to rotate simultaneously, and during the rotation of the turntable 1204, the lever 1206 can be driven to perform a stable circular motion, and under the movement action of the lever 1206, it can stably move into the guide groove 1209 on the second limit plate 1208, and can move the second limit plate 1208 to move, and the lever 1206 can move the second limit plate 1208 to automatically and stably rotate intermittently by moving the four guide grooves 1209 one by one, and each time it rotates 90°, and when the lever 1206 drives the second limit plate 1208 to rotate through the guide groove 1209, the depression on the first limit plate 1205 and the second limit plate 1208 are aligned with each other. When the second limiting plate 1208 stops rotating, the side end surface of the first limiting plate 1205 will fit with the side end surface of the second limiting plate 1208, thereby limiting the second limiting plate 1208, avoiding shaking when the second limiting plate 1208 stops rotating, and ensuring the stability of the intermittent rotation of the second limiting plate 1208. Under the 90° intermittent rotation of the second limiting plate 1208, the positioning rod 1211 on the transmission shaft 1207 can drive the mounting plate 1214 to rotate synchronously and intermittently, thereby driving the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 to switch positions conveniently and stably.

[0056] Therefore, in the initial state of the mounting plate 1214, the first flow guide tube 1217 is located directly above the centrifuge tube 10. The staff can drive the connecting plate 1202 to move downward by driving the hydraulic rod 1201. At this time, under the movement of the connecting plate 1202, the mounting plate 1214 on the transmission shaft 1207 can drive the first flow guide tube 1217 to be stably inserted into the centrifuge tube 10. Then the staff can drop the lymphocyte separation liquid into the first flow guide tube 1217. Under the guidance of the first flow guide tube 1217, the lymphocyte separation liquid is injected into the centrifuge tube 10 through the hole in the center of the partition 11.

[0057] Then the staff can drive the hydraulic rod 1201 to drive the connecting plate 1202 to move upward and reset, drive the first guide tube 1217 to move away from the centrifuge tube 10, and then drive the mounting plate 1214 to rotate intermittently by 90 degrees. At this time, the second guide tube 1218 is located directly above the centrifuge tube 10, and the hydraulic rod 1201 is used to drive the connecting plate 1202 to move downward, and drive the second guide tube 1218 to move downward into the centrifuge tube 10. At this time, the staff can drop the diluted sample into the second guide tube 1218. Under the guidance of the second guide tube 1218, the sample can be added to the centrifuge tube along the tube wall. In the centrifugal tube 10, the sample will be mixed with the lymphocyte separation solution above the partition 11, but it will not affect the separation effect. Similarly, after the sample is added, the hydraulic rod 1201 is driven again to drive the connecting plate 1202 to move upward and reset, and drive the second guide tube 1218 to move away from the centrifugal tube 10. At this time, the staff can drive the first servo motor 901. Under the driving action of the first servo motor 901, the second plug tube 904 on the rotating plate 902 can drive the rotating plate 902 and the positioning plate 905 to rotate simultaneously, thereby driving the clamped and fixed centrifugal tube 10 to rotate synchronously for centrifugal treatment;

[0058] After the centrifugation is completed, an upper layer of dilution liquid containing MNCs is generated in the upper layer of the centrifuge tube 10, and the first servo motor 901 can be turned off. At this time, the mounting plate 1214 is rotated intermittently by 90 degrees again. At this time, the third guide tube 1219 is located directly above the centrifuge tube 10. The hydraulic rod 1201 is used to drive the connecting plate 1202 to move downward, and drive the third guide tube 1219 to move downward into the centrifuge tube 10. At this time, the staff can rotate the second threaded rod 1225 on the second filter plate 1224 at the top of the third guide tube 1219, and then the second threaded rod 1225 on the second filter plate 1224 can be rotated to move the third guide tube 1219 downward. The threaded rod 1225 can drive the fourth rubber piston 1226 at the bottom to move upward stably inside the third guide tube 1219 under the action of the threaded rotation. Under the action of the movement of the fourth rubber piston 1226, the upper dilution liquid containing MNCs inside the centrifuge tube 10 can be sucked through the third guide tube 1219. After the upper dilution liquid containing MNCs is sucked into the third guide tube 1219, the hydraulic rod 1201 drives the connecting plate 1202 to move upward and reset, thereby driving the third guide tube 1219 to move away from the centrifuge tube 10.

[0059] Then the staff can reversely rotate the first threaded rod 908. Under the reverse rotation of the first threaded rod 908, the second rubber piston 910 can be driven to move upward and reset through the threaded sleeve rod 909, and then the third plug tube 913 can be evacuated through the first air guide tube 911 and the second air guide tube 912. Under the evacuation of the third plug tube 913, the second plug rod 915 on the third rubber piston 914 can drive the clamping plate 916 to move away from the centrifuge tube 10. At this time, the staff can take out the used centrifuge tube 10 and insert the new centrifuge tube 10 into the through groove 906 on the positioning plate 905. Then the staff can forwardly rotate the first threaded rod 908 again, and the new centrifuge tube 10 can be conveniently and stably clamped and fixed by each clamping plate 916.

[0060] Then the staff can reversely rotate the second threaded rod 1225. Under the reverse threaded rotation of the second threaded rod 1225, the fourth rubber piston 1226 at the bottom can be driven to move downward and reset inside the third guide tube 1219, so that the upper layer of diluent sucked inside the third guide tube 1219 can be stably pushed into the centrifuge tube 10. At this time, the mounting plate 1214 is intermittently rotated by 90° again. At this time, the fourth guide tube 1220 is located directly above the centrifuge tube 10. At this time, under the diversion effect of the fourth guide tube 1220, the staff can drip D-PBS (2μM EDTA) and 2% FBS into the fourth guide tube 1220, and guide them into the centrifuge tube 10, and drive the new centrifuge tube 10 to centrifuge through the drive of the first servo motor 901, wash the cells to remove the platelets in the separated MNCs, so as to obtain the best cell sorting effect and complete the cell separation work;

[0061] After the cell separation work is completed, the staff can push the stopper 1213 to move into the positioning rod 1211. Without the obstruction of the stopper 1213, the staff can move the mounting plate 1214 downward and detach it from the positioning rod 1211. Then the staff can bring a new mounting plate 1214, which is equipped with a new first flow guide tube 1217, a second flow guide tube 1218, a third flow guide tube 1219 and a fourth flow guide tube 1220. The staff only needs to insert the positioning groove 1215 on the mounting plate 1214 into the positioning rod 1211. During the insertion of the mounting plate 1214, The stopper 1213 can be in contact with the inclined surface of the end of the stopper 1213. During the insertion of the mounting plate 1214, the stopper 1213 can be pushed to automatically move to the inside of the positioning rod 1211 until the mounting plate 1214 passes over the stopper 1213. At this time, the stopper 1213 can automatically move outward and reset under the elastic action of the third spring 1212. The stopper 1213 can be easily and conveniently installed with the baffle 1210 and the positioning rod 1211 to complete the clamping installation of the new mounting plate 1214 and ensure that the new mounting plate 1214 is in the initial state. For the disassembled mounting plate 1214, the staff only needs to pull the clamping rod 1211 on the mounting plate 1214 outward. 223, until the clamping rod 1223 moves out of the clamping slot 1221, the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 can be easily disassembled, thereby ensuring the convenience of subsequent cleaning of the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220. Similarly, when the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 need to be clamped and installed, it is only necessary to pull the clamping rod 1223 outwards in advance, and then the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 can be easily disassembled. The guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 are inserted into the installation groove 1216 on the installation plate 1214. Then, the staff only needs to loosen the clamping rod 1223. At this time, the clamping rod 1223 can automatically engage with the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220 under the elastic action of the fourth spring 1222, so as to conveniently complete the clamping installation of the first guide tube 1217, the second guide tube 1218, the third guide tube 1219 and the fourth guide tube 1220;

[0062] After the cell separation work is completed, the new centrifuge tube 10 can be disassembled and taken out. The staff uses the separated cell solution to prepare a single cell suspension and transfers the prepared single cell suspension to the flow tube. Then the staff can insert the flow tube into the through groove 906 on the positioning plate 905 and clamp the flow tube through each clamping plate 916. Then, through the same operation as above, the sorting reagent is added to the flow tube through the first guide tube 1217, and centrifugal mixing is performed. Then, through the same operation as above, ImunoSep is added to the flow tube through the second guide tube 1218. TM Buffer, wash the cells, and then continue centrifugation; then, through the same operation as above, the supernatant inside the flow tube is aspirated through the third flow tube 1219, and ImunoSep is added into the flow tube through the second flow tube 1218 TM Buffer resuspends the cells to their initial volume, then adds the sorting reagent through the fourth flow conduit 1220, and centrifuges to mix;

[0063] Then the staff can disassemble and take out the flow tube, and put it into the magnetic immunoseparator 14 on the support plate 13, and let it stand at room temperature for 5 minutes. At the same time, put the new centrifuge tube 10 into the through groove 906 on the positioning plate 905, and then the staff can rotate the third threaded rod 16 on the support plate 13 until it moves out of the threaded hole 15 on the fixed net frame 1, and then the magnetic immunoseparator 14 can be disassembled. At this time, the staff can pick up the magnetic immunoseparator 14 and the flow tube together, and quickly pour the supernatant in the flow tube into the new centrifuge tube 10. The flow tube should not be inverted for more than 2 seconds, and then it will be restored to an upright position to complete the purification of uterine decidual NK cells. After the use of the magnetic immunoseparator 14 is finished, the third threaded rod 16 on the support plate 13 can be rotated in the opposite direction until it rotates into the threaded hole 15 on the fixed net frame 1 to complete the installation and fixation of the magnetic immunoseparator 14, so as to ensure the storage stability and safety of the magnetic immunoseparator 14 during the subsequent transportation of the device state;

[0064] After the separation and purification of uterine decidual NK cells is completed, the staff can insert the protective side panel 803 into the first limiting groove 3 on the fixed side panel 2, and before insertion, the staff needs to pull the baffle 7 to the middle in advance. After the protective side panel 803 is inserted into the first limiting groove 3 on the fixed side panel 2, the staff can release the baffle 7. At this time, the baffle 7 can block the push plate 804 on the protective side panel 803 to prevent the protective side panel 803 on the push plate 804 from moving upward and separating from the fixed side panel 2, thereby ensuring the stability of the snap-fit ​​installation of the protective side panel 803. By using the snap-fit ​​installation of the protective side panels 803 on both sides, in conjunction with the protective top panel 801 and the fixed side panel 2, The device as a whole can be conveniently and stably sealed and protected, and the convenience and stability of the subsequent transportation of the device can be ensured; and during the insertion and installation process of the protective side plate 803, the first plug rod 808 can be pushed downward by the push plate 804, and then the first rubber piston 807 can be pushed downward. At this time, under the movement of the first rubber piston 807, the air inside the first plug tube 805 can be pushed into the rubber suction cups 810 on the connecting tube 809. At this time, the negative pressure inside the rubber suction cups 810 disappears, and the device as a whole can automatically release the adsorption and fixation after the work is completed and the protection work is completed, thereby ensuring the convenience of the subsequent transportation of the device as a whole.

[0065] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A device for efficiently separating and purifying uterine decidual NK cells, characterized in that: The invention comprises a fixed net frame (1), a centrifuge tube (10) and a support plate (13), wherein a fixed side plate (2) is welded and fixed on the top surface of the fixed net frame (1), a first limiting groove (3) is provided on the fixed side plate (2), a second limiting groove (4) is provided through the fixed side plate (2), a connecting plate (5) is welded and fixed on the fixed side plate (2), the connecting plate (5) is welded and fixed to one end of a first spring (6), the other end of the first spring (6) is welded and fixed to one end of a blocking rod (7), the blocking rod (7) is slidably connected to the connecting plate (5), a multifunctional protective component (8) is installed on the fixed net frame (1), a high-efficiency positioning component (9) is installed on the top of the fixed net frame (1), and the centrifuge tube (10) is provided on the top of the fixed net frame (1). (10) is fixedly connected with a partition (11), an integrated processing component (12) is installed on the top of the fixed net frame (1), a magnetic immune sorter (14) is installed and fixed on the top surface of the support plate (13), threaded holes (15) are opened through the top of the support plate (13) and the fixed net frame (1), a third threaded rod (16) is threadedly connected to the threaded hole (15), and the multifunctional protective component (8) includes a protective top plate (801), a fixed handle (802) is welded and fixed on the top surface of the protective top plate (801), and a protective side plate (803) is welded and fixed on the bottom surface of the protective top plate (801), and the protective side plates (803) are symmetrically distributed on the bottom of the protective top plate (801). On both sides of the protective side plate (803), a push plate (804) is welded and fixed on the side end surface, and the top end surface of the push plate (804) is in contact with the other end of the blocking rod (7). The protective side plate (803) is slidably connected in the first limiting groove (3). The push plates (804) are symmetrically distributed on both sides of the protective side plate (803). The push plates (804) are slidably connected in the second limiting groove (4). The fixed handle (802) is fixed to the top middle part of the protective top plate (801). A first plug tube (805) is welded and fixed to the fixed net frame (1). The high-efficiency positioning component (9) includes a first servo motor (901), and the first servo motor (901) is welded and fixed to the fixed In the screen frame (1), a rotating plate (902) is welded and fixed on the output shaft of the first servo motor (901), a rubber plate (903) and a second plug tube (904) are fixedly connected on the top surface of the rotating plate (902), a positioning plate (905) is welded and fixed on the top of the second plug tube (904), a through slot (906) is penetrated through the positioning plate (905), a first filter plate (907) is welded and fixed on the top of the second plug tube (904), a first threaded rod (908) is rotatably connected to the first filter plate (907), a threaded sleeve rod (909) is threadedly connected to the first threaded rod (908), and a second rubber piston (910) is fixedly connected to the bottom end of the threaded sleeve rod (909),The second rubber piston (910) is slidably connected in the second plug tube (904); the output shaft of the first servo motor (901) is fixed at the bottom center of the rotating plate (902); the rubber plate (903) is fixed at the top center of the rotating plate (902); the second plug tube (904) is symmetrically distributed on both sides of the rotating plate (902); the top surface of the second plug tube (904), the top surface of the first filter plate (907) and the top surface of the positioning plate (905) are flush; the vertical center line of the positioning plate (905), the vertical center line of the through groove (906) and the vertical center line of the rotating plate (902) are located on the same vertical line; the bottom side end of the second plug tube (904) is connected to the bottom side end of the second plug tube (904); A first air guide tube (911) is provided, the top end of the first air guide tube (911) is connected to a second air guide tube (912), the second air guide tube (912) is connected to a third plug tube (913), the third plug tube (913) and the second air guide tube (912) are both fixedly connected in the positioning plate (905), a third rubber piston (914) is slidably connected in the third plug tube (913), a second plug rod (915) is fixedly connected to the third rubber piston (914), the second plug rod (915) penetrates and is slidably connected in the third plug tube (913), a clamping plate (916) is welded and fixed at the end of the second plug rod (915), and a rubber pad (916) is fixedly connected to the clamping plate (916). 7), the integrated processing assembly (12) comprises a hydraulic rod (1201) and a mounting plate (1214), the bottom end of the hydraulic rod (1201) is mounted and fixed on the fixed net frame (1), the top end of the hydraulic rod (1201) is fixedly connected to a connecting plate (1202), a second servo motor (1203) is welded and fixed on the top surface of the connecting plate (1202), a rotating disk (1204) and a first limiting plate (1205) are welded and fixed on the output shaft of the second servo motor (1203), a shifting rod (1206) is welded and fixed on the rotating disk (1204), a transmission shaft (1207) is rotatably connected to the bottom end surface of the connecting plate (1202), and a first stop plate (1205) is welded and fixed on the transmission shaft (1207). A second limiting plate (1208) is provided, and a guide groove (1209) is provided through the second limiting plate (1208). The hydraulic rods (1201) are symmetrically distributed on both sides of the connecting plate (1202). The side end surface of the first limiting plate (1205) is in contact with the side end surface of the second limiting plate (1208). Four guide grooves (1209) are provided, and the four guide grooves (1209) are distributed at equal angles on the second limiting plate (1208). A baffle plate (1210) is welded and fixed to the bottom end of the transmission shaft (1207), and a positioning rod (1211) is welded and fixed to the bottom end surface of the baffle plate (1210). The positioning rod (1211) is welded and fixed to one end of the third spring (1212).A stopper (1213) is welded and fixed to the other end of the third spring (1212), and the stopper (1213) is slidably connected to the positioning rod (1211) in a limited position. A positioning groove (1215) and a mounting groove (1216) are provided on the mounting plate (1214), and the positioning rod (1211) is slidably connected to the positioning groove (1215). The length of the positioning rod (1211) is greater than the thickness of the mounting plate (1214), and the third springs (1212) are symmetrically distributed on both sides of the positioning rod (1211). 2) corresponding to the stopper (1213) one by one, the cross section of the stopper (1213) is a right-angle trapezoid, four mounting grooves (1216) are provided, and the four mounting grooves (1216) are distributed at equal angles on the mounting plate (1214), and the first guide tube (1217), the second guide tube (1218), the third guide tube (1219) and the fourth guide tube (1220) are slidably connected in the four mounting grooves (1216), respectively, and the mounting plate (1214) is in a "cross" shape as a whole, and the positioning groove (1215) is provided at the center of the mounting plate (1214).

2. The device for efficiently separating and purifying uterine decidual NK cells according to claim 1, characterized in that: The fixed side plates (2) are symmetrically distributed on both sides of the top of the fixed net frame (1); the first limiting grooves (3) are symmetrically distributed on both sides of the fixed side plates (2); the first limiting grooves (3) correspond one to one with the second limiting grooves (4); the width of the second limiting grooves (4) is equal to the width of the first limiting grooves (3); the connecting plates (5) are symmetrically distributed on the fixed side plates (2); the connecting plates (5) correspond one to one with the blocking rods (7) through the first springs (6).

3. The device for efficiently separating and purifying uterine decidual NK cells according to claim 1, characterized in that: The inner top surface of the first plug tube (805) is welded and fixed to the top of the second spring (806); the bottom end of the second spring (806) is fixedly connected to a first rubber piston (807); the first rubber piston (807) is slidably connected in the first plug tube (805); the top surface of the first rubber piston (807) is fixedly connected to a first plug rod (808); the first plug rod (808) penetrates and is slidably connected in the first plug tube (805); the bottom end of the first plug tube (805) is connected to a connecting tube (809); the connecting tube (809) is welded and fixed in the fixed net frame (1), the bottom of the connecting tube (809) is connected with a rubber suction cup (810), the top end surface of the first plug rod (808) is in contact with the bottom end surface of the push plate (804), the push plate (804) corresponds to the first plug tube (805) one by one through the first plug rod (808), the second spring (806) is sleeved on the first plug rod (808), the connecting tube (809) is rectangular as a whole, and the rubber suction cups (810) are equidistantly distributed at the bottom of the connecting tube (809).

4. The device for efficiently separating and purifying uterine decidual NK cells according to claim 1, characterized in that: The second air guide tube (912) is in an annular shape as a whole. The overall annular inner diameter of the second air guide tube (912) is larger than the diameter of the through groove (906). Four third plug tubes (913) are provided. The four third plug tubes (913) are distributed at equal angles on the annular inner wall of the second air guide tube (912). The third plug tubes (913) correspond to the third rubber pistons (914) one by one. The third rubber pistons (914) correspond to the clamping plates (916) one by one through the second plug rod (915). The second plug rod (915) is fixed to the middle part of the side end of the clamping plate (916). The cross section of the clamping plate (916) is in an arc shape. The second plug tube (904) and the third plug tube (913) are both rectangular tubes.

5. The device for efficiently separating and purifying uterine decidual NK cells according to claim 1, characterized in that: The side ends of the first flow guide tube (1217), the second flow guide tube (1218), the third flow guide tube (1219) and the fourth flow guide tube (1220) are all provided with a clamping groove (1221); the mounting plate (1214) is welded and fixed to one end of a fourth spring (1222); the other end of the fourth spring (1222) is welded and fixed to a clamping rod (1223); the clamping rod (1223) is slidably connected to the mounting plate (1214); the end of the clamping rod (1223) The third flow guide tube (1219) is connected in a card-engaged manner in the card slot (1221), and the card slot (1221) corresponds to the card rod (1223) in a one-to-one manner. A second filter plate (1224) is welded and fixed to the top of the third flow guide tube (1219), and a second threaded rod (1225) is threadedly connected to the second filter plate (1224). A fourth rubber piston (1226) is rotatably connected to the bottom end of the second threaded rod (1225), and the fourth rubber piston (1226) is slidably connected in the third flow guide tube (1219).

Images