Method for rapidly quantifying physiological function difference of plant leaves

Abstract

The invention discloses a method for rapidly quantifying physiological function differences of plant leaves, and belongs to the technical fields of plant health management, germplasm resource evaluation, intelligent agriculture, plant ecological restoration and the like. According to the method, physiological capacitance, physiological resistance and physiological impedance under different clamping forces are calculated, and physiological capacitive reactance and physiological inductive reactance of plant leaves are calculated; respectively constructing models of physiological capacitance, physiological resistance, physiological impedance, physiological capacitive reactance and physiological inductive reactance of the leaf changing along with the clamping force; calculating inherent physiological resistance, inherent physiological impedance, inherent physiological capacitive reactance, inherent physiological inductive reactance, specific effective thickness, intracellular moisture metabolism, intracellular nutrition metabolism, operation of various intracellular dielectric substances, energy metabolism of various intracellular dielectric substances and metabolic activity of plant leaves by using parameters of the models, and forming a plant physical examination report according to the data. The physiological functions of the to-be-tested leaves of the living plant are evaluated, the difference of the physiological functions of the to-be-tested leaves of the plant is given, and the method has significance and effects on plant growth and development and yield formation.

Description

Technical Field

[0001] The present invention belongs to the fields of plant biotechnology, plant health management, germplasm resource evaluation, plant breeding technology, smart agriculture, smart forestry, and plant ecological restoration technology, and specifically relates to a method for rapidly quantifying differences in physiological functions of plant leaves. Background Art

[0002] Leaves are the most important functional organs of plants. They are energy converters that convert solar energy into chemical energy and producers of organic matter. As the timing of plant growth and development varies, the physiological functions of different leaves of the same plant or the same leaf at different growth and development stages are also significantly different. The differentiation of the physiological functions of plant leaves determines the structural formation and functional realization of plant tissues and organs.

[0003] Different leaves have different divisions of labor for the growth and development of plants. The flag leaf of wheat plays a decisive role in the yield of wheat grains because of the large number of chloroplasts in the cells and the large number of grana thylakoids in the chloroplasts; the flag leaf of rice also plays a pivotal role in the yield and quality of rice; and their second leaf, third leaf and fourth leaf play an important role in their yield and quality. The leaves of the flag / flag leaf group (flag / flag leaf and second leaf, third leaf and fourth leaf), stem leaf group and near-root leaf group play a leading role in the formation of grains, the development of stems and the growth of roots, respectively. Therefore, the rapid quantification of the differences in the physiological functions of different leaves of different plants, different leaves of the same plant (different environments or the same environment) or the same leaf at different growth and development stages according to the time series will provide basic data for many fields such as plant health management, germplasm resource evaluation, plant breeding, smart agriculture, smart forestry and plant ecological restoration technology.

[0004] Before the leaves of plants unfold, they need to obtain nutrients from other leaves for their growth. Therefore, young leaves can only play a functional role when they unfold and become net producers. In other words, the unfolded leaves are the functional leaves of plants. The unfolded leaves of plants are all mature leaves. Their cells all have central vacuoles. In mesophyll cells, vacuoles and cytoplasm occupy most of the space in the cells. Their water absorption method is mainly osmotic absorption. Whether it is cells or organelles, they are all covered with cell membranes on the outside. The cell membrane is mainly composed of lipids (mainly phospholipids) (accounting for about 50% of the total cell membrane), proteins (accounting for about 40% of the total cell membrane) and carbohydrates (accounting for about 2%-10% of the total cell membrane). Among them, proteins and lipids are the main ones. The phospholipid bilayer is the basic support of the cell membrane. Under the electron microscope, it can be divided into three layers, that is, there is an electron-dense band (hydrophilic part) about 2.5nm thick on both sides of the membrane, and a transparent band (hydrophobic part) with a thickness of 2.5nm in the middle. Therefore, the cell (organelle) can be regarded as a concentric spherical capacitor, except that this capacitor becomes a complex capacitor with both inductor and inductor functions due to the peripheral proteins and intrinsic proteins on the membrane. Therefore, the electrophysiological characteristics of plant leaf cells are closely related to the material metabolism and energy metabolism of plant leaves, and the electrical behavior of leaf cells can comprehensively and scientifically characterize the physiological functions of plants. Summary of the invention

[0005] The purpose of the present invention is to provide a method for rapidly quantifying the differences in physiological functions of plant leaves, which overcomes the defect that traditional plant sensors are difficult to measure plant electrical dynamics information, and uses a plant electrical behavior measurement system to measure cell dielectric material dynamics indicators, and use these indicators to characterize the intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and energy metabolism and metabolic activity of various dielectric substances in the cell, and form a physical examination report; based on the plant physical examination report, the physiological functions of plant leaves are evaluated, and the significance and role of specialized physiological function leaves in plant growth and development and yield formation are diagnosed, providing technical support for plant physiological breeding, smart agriculture, etc. In order to solve the above technical problems, the specific technical solutions adopted by the present invention are as follows:

[0006] A method for rapidly quantifying differences in plant leaf physiological functions, comprising the following steps:

[0007] Step 1: quickly measure the physiological capacitance, physiological resistance and physiological impedance of each leaf of the plant to be tested under different clamping forces and calculate the physiological capacitance and physiological inductance of the plant leaves; respectively construct models of the physiological capacitance, physiological resistance, physiological impedance, physiological capacitance and physiological inductance of each leaf of the plant to be tested changing with the clamping force; and obtain the parameters of each model;

[0008] Step 2: Calculate the intrinsic physiological resistance, intrinsic physiological impedance, intrinsic physiological capacitance, intrinsic physiological inductance and specific effective thickness of the plant leaves, as well as the intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and intracellular energy metabolism of various dielectric substances, using the parameters of each model of each leaf to be tested in step 1, and then obtain the metabolic vitality of the plant, wherein the intracellular operation of various dielectric substances includes the dielectric substance transfer number Kn in response to the physiological resistance. R , Dielectric material transfer number Kn in response to physiological capacitive reactance XC , Dielectric material transfer number Kn in response to physiological reactance XL and the dielectric mass transfer number Kn in response to physiological impedance Z , resistive material conductivity ICF R , resistive material conduction resistance ICR R , Capacitive Material Conductivity ICF C , Capacitive material conduction resistance ICR C , Inductive Material Conductivity ICF L , Inductive material conduction resistance ICR L , total dielectric conductivity ICF Z and total dielectric conductivity resistance ICR Z ;

[0009] Step 3, forming a physical examination report with the data calculated in step 2;

[0010] Step 4: Give the biological and physiological significance of each indicator in the physical examination report;

[0011] Step five, calibrate the physiological function values ​​of the leaves on each leaf to be tested, rank the functions and functional rankings of each leaf, and diagnose the significance and role of the specialized physiological function leaves on plant growth and development and yield formation.

[0012] Furthermore, in the step 1, the model of the physiological resistance of the plant leaf changing with the clamping force, Where R is the physiological resistance, E is the electromotive force, and E 0 is the standard electromotive force, R0 is the ideal gas constant, T is the temperature, C i is the concentration of dielectric substances in the cell membrane that responds to physiological resistance, C o is the dielectric substance concentration outside the cell membrane that responds to the physiological resistance, and f0 is the dielectric substance concentration inside the cell membrane that responds to the physiological resistance C i The proportional coefficient between the conversion and physiological resistance, the total amount of dielectric material C inside and outside the membrane that responds to physiological resistance T =C i +C o , F0 is Faraday's constant, n Ris the number of dielectric substances transferred in response to physiological resistance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological resistance of plant leaves changing with the clamping force can be transformed into Wherein y1, k1 and b1 are parameters of the model, and the calculation method of the intrinsic physiological resistance IR of the plant leaf is: IR=y1+k1.

[0013] Furthermore, in the step 1, the model of the physiological impedance of the plant leaf changing with the clamping force, Where Z is physiological impedance, E is electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, T is the temperature, Q i is the concentration of dielectric substances in the cell membrane that responds to physiological impedance, Q o is the dielectric substance concentration outside the cell membrane that responds to the physiological impedance, and J0 is the dielectric substance concentration Q inside the cell membrane that responds to the physiological impedance i The proportional coefficient between the conversion and impedance, the total amount of dielectric material inside and outside the membrane that responds to physiological impedance Q = Q i +Q o , F0 is Faraday's constant, n Z is the number of dielectric substances transferred in response to physiological impedance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological impedance of plant leaves changing with the clamping force can be transformed into Wherein y2, k2 and b2 are parameters of the model, and the calculation method of the inherent physiological impedance IZ of the plant leaf is: IZ=y2+k2.

[0014] Furthermore, in the step 1, the model of the physiological capacitive reactance of the plant leaf changing with the clamping force, Where Xc is the physiological capacitive reactance, E is the electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, T is the temperature, X i is the concentration of dielectric substances in the cell membrane that responds to physiological capacitance, X o is the dielectric substance concentration outside the cell membrane that responds to the physiological capacitance, and L0 is the dielectric substance concentration inside the cell membrane that responds to the physiological capacitance. iThe proportional coefficient between the conversion and physiological capacitance, the total amount of dielectric material inside and outside the membrane that responds to physiological capacitance X = X i +X o , F0 is Faraday's constant, n XC is the number of dielectric material transfers in response to physiological capacitive reactance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of the plant cell, P is the pressure on the plant cell, and the pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological capacitive reactance of plant leaves changing with the clamping force can be transformed into Wherein y3, k3 and b3 are parameters of the model, and the calculation method of the inherent physiological capacitive reactance IXc of the plant leaf is: IXc=y3+k3.

[0015] Furthermore, in the step 1, the model of the physiological inductive reactance of the plant leaves changing with the clamping force, Where Xl is the physiological inductive reactance, E is the electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, T is the temperature, M i is the concentration of dielectric substances in the cell membrane that respond to physiological reactance, M o is the dielectric substance concentration outside the cell membrane that responds to physiological reactance, and P0 is the dielectric substance concentration M inside the cell membrane that responds to physiological reactance i The proportional coefficient between the conversion and physiological inductance, the total amount of dielectric material M inside and outside the membrane that responds to physiological inductance T =M i +M o , F0 is Faraday's constant, n XL

[0016] is the number of dielectric substances transferred in response to physiological inductive reactance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Find out, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf, let The model of the physiological inductive reactance of plant leaves changing with the clamping force can be transformed into Wherein y4, k4 and b4 are parameters of the model, and the calculation method of the inherent physiological resistance IXl of the plant leaves is: IXl=y4+k4.

[0017] Furthermore, in step 2, the dielectric material transfer number Kn of the response physiological resistance R The calculation method of Kn is: R= lnk1-lny1; Dielectric material transfer number Kn in response to physiological capacitive reactance XC The calculation method of Kn is: XC = lnk3-lny3; Dielectric material transfer number Kn in response to physiological reactance XL The calculation method of Kn is: XL = lnk4-lny4; Dielectric material transfer number Kn in response to physiological impedance Z The calculation method of Kn is: Z =lnk2-lny2; resistive material conductivity ICF R The calculation method is: Resistive material conduction resistance ICR R The calculation method is: ICR R =-b1k1, capacitive material conduction force ICF C The calculation method is: Capacitive material conduction resistance ICR C The calculation method is: ICR C =-b3k3, ICF of inductive material conduction L The calculation method is: Inductive material conduction resistance ICR L The calculation method is: ICR L =-b4k4; total dielectric conductivity ICF Z The calculation method is: Total dielectric conductivity resistance ICR Z The calculation method is: ICR Z =-b2k2.

[0018] Furthermore, in step 2, the energy metabolism of various dielectric substances in the cell includes a unit metabolic energy ΔG based on resistance. R-U and the metabolic energy ΔG based on resistance R , Unit metabolic energy ΔG based on impedance Z-U and impedance-based metabolic energy ΔG Z , Unit metabolic energy ΔG based on capacitive reactance Xc-U and metabolic energy ΔG based on capacitive reactance Xc , Unit metabolic energy ΔG based on inductive reactance Xl-U and the metabolic energy ΔG based on inductive resistance Xl ; Unit metabolic energy ΔG based on resistance R-U The calculation method is: Metabolic energy ΔG based on resistance R The calculation method is: ΔG R =ΔG R-U d; Unit metabolic energy ΔG based on impedance Z-U The calculation method is: Impedance-based metabolic energy ΔG Z The calculation method is: ΔG Z =ΔG Z-U d; Unit metabolic energy ΔG based on capacitive reactance Xc-U The calculation method is: Metabolizable energy ΔG based on capacitive reactance Xc The calculation method is: ΔG Xc =ΔG Xc-U d; Unit metabolic energy ΔG based on inductive reactance Xl-U The calculation method is: Metabolizable energy ΔG based on inductive reactance Xl The calculation method is: ΔG Xl =ΔG Xl-U d.

[0019] Furthermore, in step 2, the calculation method of the plant leaf specific effective thickness d is: Wherein U is the test voltage, and h is the parameter of the model of the physiological capacitance of the plant leaf changing with the clamping force.

[0020] Furthermore, in the step 2, the intracellular nutrient metabolism of the plant includes the intracellular nutrient transfer rate INTR, the intracellular nutrient utilization efficiency NUE, the low nutrient tolerance capacity RLN, the active transport unit flow UAF, the intracellular nutrient active transport capacity NAC, and the intracellular nutrient transport capacity NTC; the intracellular nutrient transfer rate INTR and the intracellular water transfer rate IWTR are similar in concept and have the same value; the calculation formula of the intracellular nutrient utilization efficiency NUE is: The calculation formula for low nutrition tolerance RLN is: The calculation formula of active transport unit flow UAF is: The calculation formula of intracellular nutrient active transport capacity NAC is: NAC = UAF × INTR; the calculation formula of intracellular nutrient transport capacity NTC is: NTC = UNF × INTR, where UNF is the nutrient unit flow, which can be expressed as:

[0021] Furthermore, the step five specifically includes:

[0022] S501, calibrating the values ​​of leaf intracellular water metabolism on each leaf to be tested, subdividing and ranking the intracellular water metabolism functions of each leaf, and giving the differences in the intracellular water metabolism functions of each leaf;

[0023] S502, calibrating the values ​​of leaf intracellular nutrient metabolism on each leaf to be tested, subdividing and ranking the intracellular nutrient metabolism functions of each leaf, and giving the differences in the intracellular nutrient metabolism functions of each leaf;

[0024] S503, calibrating the values ​​of the operation of various dielectric substances in the leaf cells on each leaf to be tested, subdividing and ranking the operation functions of various dielectric substances in the cells of each leaf, and giving the differences in the operation functions of various dielectric substances in the cells of each leaf;

[0025] S504, calibrating the values ​​of energy metabolism of various dielectric substances in leaf cells on each leaf to be tested, subdividing and ranking the energy metabolism functions of various dielectric substances in cells of each leaf, and giving the differences in energy metabolism functions of various dielectric substances in cells of each leaf;

[0026] S505, calibrating the value of the leaf metabolic activity on each leaf to be tested, subdividing and ranking the metabolic activity of each leaf, and providing the difference of the overall metabolic function of each leaf;

[0027] S506, based on the differences among leaves in intracellular water metabolism function, intracellular nutrient metabolism function, intracellular various dielectric substance operation function, intracellular various dielectric substance energy metabolism function and overall plant metabolism function.

[0028] The present invention has beneficial effects: the present invention obtains the electrical parameters such as inductance, capacitance, impedance, etc. of living leaves under the action of high-frequency low-voltage electric field by online pressure coupling, constructs a physical model between electrical parameters and pressure based on the Gibbs free energy equation and the Nernst equation, constructs a leaf cell dielectric material kinetic model based on the physical model and electrical principle between electrical parameters and pressure, and derives the intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and energy metabolism and metabolic activity of various dielectric substances in the plant based on the leaf cell dielectric material kinetic model and biophysics and plant physiology principles. The present invention will be able to quickly obtain the differences in physiological functions of different leaves, and provide essential first-hand data for plant production, plant health management, plant germplasm resource evaluation, plant physiological breeding, smart agriculture and forestry, and plant ecological restoration.

[0029] This application uses a plant electrical behavior measurement system to measure the cellular dielectric material dynamics index, overcoming the defect that traditional plant sensors are difficult to measure plant electrical dynamics information. It proposes to use electrical parameters such as inductive reactance, capacitance, and impedance of living leaves to quantitatively obtain various dielectric material operation indicators and various dielectric material energy metabolism indicators in cells, including the dielectric material transfer number Kn that responds to physiological resistance. R , Dielectric material transfer number Kn in response to physiological capacitive reactance XC , Dielectric material transfer number Kn in response to physiological reactance XL and the dielectric mass transfer number Kn in response to physiological impedance Z , resistive material conductivity ICF R , resistive material conduction resistance ICR R , Capacitive Material Conductivity ICFC , Capacitive material conduction resistance ICR C , Inductive Material Conductivity ICF L , Inductive material conduction resistance ICR L , total dielectric conductivity ICF Z and total dielectric conductivity resistance ICR Z , Unit metabolic energy ΔG based on resistance R-U and the metabolic energy ΔG based on resistance R , Unit metabolic energy ΔG based on impedance Z-U and impedance-based metabolic energy ΔG Z , Unit metabolic energy ΔG based on capacitive reactance Xc-U and metabolic energy ΔG based on capacitive reactance Xc , Unit metabolic energy ΔG based on inductive reactance Xl-U and the metabolic energy ΔG based on inductive resistance Xl It objectively reflects the ability of leaves to flow (distribute) matter and energy, and plays an indispensable and key role in the evaluation of plant physiological functions.

[0030] The present invention can quickly and completely form a physical examination report of the plant with biophysical indicators without the need for separation from the body, and the physical examination report truly reflects the immediate physiological status and physiological function of the plant.

[0031] The present invention can quickly and completely quantify the intracellular water metabolism, intracellular nutrient metabolism, intracellular various dielectric substance operations, intracellular various dielectric substance energy metabolism and metabolic activity of different leaves online. It is simple, fast, short in time, small in workload, wide in applicability, and requires simple instrument operation.

[0032] The present invention can not only quickly obtain the differences in the physiological functions of different leaves, but also quickly evaluate and diagnose the specialized physiological functions of plant leaves. DETAILED DESCRIPTION

[0033] The basic principle of the present invention is:

[0034] Cells are complex capacitors that function as both inductors and reactances. The electrophysiological characteristics of plant leaf cells are closely related to the material metabolism and energy metabolism of plant leaves, and the electrical behavior of leaf cells can comprehensively and scientifically characterize plant physiological functions. The present invention obtains electrical parameters such as leaf inductance, capacitance, and impedance online under the action of a pressure-coupled high-frequency low-voltage electric field, constructs a physical model between electrical parameters and pressure based on the Gibbs free energy equation and the Nernst equation, constructs a leaf cell dielectric material kinetic model based on the physical model between electrical parameters and pressure and electrical principles, and derives the plant's intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and energy metabolism and metabolic activity of various dielectric substances in the cell based on the leaf cell dielectric material kinetic model and biophysics and plant physiology principles.

[0035] A method for rapidly quantifying differences in plant leaf physiological functions, comprising the following steps:

[0036] Step 1: clamp the leaves between the parallel plate capacitance sensors of the measuring device, obtain the physiological capacitance, physiological resistance and physiological impedance under different clamping forces in the parallel mode under the action of the pressure-coupled high-frequency low-voltage electric field, and calculate the physiological capacitive reactance and physiological inductive reactance of the plant leaves; respectively construct models of the changes of physiological capacitance, physiological resistance, physiological impedance, physiological capacitive reactance and physiological inductive reactance of each leaf to be tested of the plant with the clamping force; and obtain the parameters of each model.

[0037] Step 2, using the parameters of each model of each leaf to be tested in step 1, calculate the inherent physiological resistance, inherent physiological impedance, inherent physiological capacitance, inherent physiological inductance and specific effective thickness of the plant leaves, as well as the intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and intracellular energy metabolism of various dielectric substances, thereby obtaining the metabolic vitality of the plant.

[0038] Among them, plant intracellular water metabolism includes: intracellular water holding capacity IWHC, intracellular water use efficiency IWUE, intracellular water holding time IWHT and intracellular water transfer rate IWTR;

[0039] Plant intracellular nutrient metabolism includes: intracellular nutrient transfer rate INTR, intracellular nutrient utilization efficiency NUE, low nutrient tolerance RLN, active transport unit flow UAF, intracellular nutrient active transport capacity NAC, intracellular nutrient transport capacity NTC; it should be noted that the intracellular nutrient transfer rate INTR and the intracellular water transfer rate IWTR are similar in concept and have the same value;

[0040] The operation of various dielectric substances in cells includes: the dielectric substance transfer number Kn in response to physiological resistance R , Dielectric material transfer number Kn in response to physiological capacitive reactance XC , Dielectric material transfer number Kn in response to physiological reactance XLand the dielectric mass transfer number Kn in response to physiological impedance Z , resistive material conductivity ICF R , resistive material conduction resistance ICR R , Capacitive Material Conductivity ICF C , Capacitive material conduction resistance ICR C , Inductive Material Conductivity ICF L , Inductive material conduction resistance ICR L , total dielectric conductivity ICF Z and total dielectric conductivity resistance ICR Z ;

[0041] Energy metabolism of various dielectric substances in cells includes: Unit metabolic energy ΔG based on resistance R-U and the metabolic energy ΔG based on resistance R , Unit metabolic energy ΔG based on impedance Z-U and impedance-based metabolic energy ΔG Z , Unit metabolic energy ΔG based on capacitive reactance Xc-U and metabolic energy ΔG based on capacitive reactance Xc , Unit metabolic energy ΔG based on inductive reactance Xl-U and the metabolic energy ΔG based on inductive resistance Xl ;

[0042] Metabolic activity includes: leaf metabolic flux MF, leaf metabolic rate MR and relative metabolic activity MA.

[0043] Step three, forming a physical examination report with the data calculated in step two.

[0044] Step 4: Based on the physical examination report formed by the physiological function index items calculated in step 2, the biological significance and physiological function significance of each index in the physical examination report are given, as shown in Table 1.

[0045] Table 1. Biological and physiological significance of leaf cell dielectric dynamics indices derived from the leaf cell dielectric dynamics model and biophysics and plant physiology principles.

[0046]

[0047]

[0048]

[0049]

[0050] The flow (distribution) of matter and energy is an important process of life activities. The movement of various dielectric substances in cells can reflect the movement process of different substances and the source-sink relationship. The energy metabolism of various dielectric substances in cells can reflect the ability to use and distribute energy. These two functions are essential and critical to plant physiological activities.

[0051] The operation indexes of various dielectric substances in cells, the energy metabolism indexes of various dielectric substances in cells, the inherent physiological resistance, inherent physiological impedance, inherent physiological capacitance, inherent physiological inductance and specific effective thickness of plant leaves, as well as the intracellular water metabolism, intracellular nutrient metabolism and metabolic activity of leaves are organized together into a physical examination report to rank the functions and functional rankings of leaves, which will help to systematically, comprehensively and quickly evaluate and diagnose the specialized physiological functions of plant leaves.

[0052] Step 5, calibrating the physiological function values ​​of the leaves on each leaf to be tested, sorting the functions and functions of each leaf, and diagnosing the significance and role of the specialized physiological function leaves on plant growth and yield formation, specifically including: Step 5 specifically includes:

[0053] S501, calibrating the values ​​of leaf intracellular water metabolism on each leaf to be tested, subdividing and ranking the intracellular water metabolism functions of each leaf, and giving the differences in the intracellular water metabolism functions of each leaf;

[0054] S502, calibrating the values ​​of leaf intracellular nutrient metabolism on each leaf to be tested, subdividing and ranking the intracellular nutrient metabolism functions of each leaf, and giving the differences in the intracellular nutrient metabolism functions of each leaf;

[0055] S503, calibrating the values ​​of the operation of various dielectric substances in the leaf cells on each leaf to be tested, subdividing and ranking the operation functions of various dielectric substances in the cells of each leaf, and giving the differences in the operation functions of various dielectric substances in the cells of each leaf;

[0056] S504, calibrating the values ​​of energy metabolism of various dielectric substances in leaf cells on each leaf to be tested, subdividing and ranking the energy metabolism functions of various dielectric substances in cells of each leaf, and giving the differences in energy metabolism functions of various dielectric substances in cells of each leaf;

[0057] S505, calibrating the value of the leaf metabolic activity on each leaf to be tested, subdividing and ranking the metabolic activity of each leaf, and providing the difference of the overall metabolic function of each leaf;

[0058] S506, based on the differences among leaves in intracellular water metabolism function, intracellular nutrient metabolism function, intracellular various dielectric substance operation function, intracellular various dielectric substance energy metabolism function and overall plant metabolism function.

[0059] The method for rapid quantification of differences in plant leaf physiological functions is described in detail as follows:

[0060] The calculation formula of plant leaf physiological capacitance is: Where Xc is the physiological capacitive reactance of plant leaves, Cp is the physiological capacitance of plant leaves, f is the test frequency, and π is the ratio of pi, which is equal to 3.1416.

[0061] Since the physiological resistance, physiological impedance and physiological capacitance of plant leaves are measured in parallel mode, the calculation formula of the physiological reactance of plant leaves is: Where Xl is the physiological inductive reactance of plant leaves, Xc is the physiological capacitive reactance of plant leaves, Z is the physiological impedance of plant leaves, and R is the physiological resistance of plant leaves.

[0062] The Gibbs free energy equation is expressed as ΔG = ΔH + PV, and the energy formula of the capacitor is expressed as W is the energy of the capacitor, which is equal to the work converted by Gibbs free energy ΔG, that is, W = ΔG; ΔH is the internal energy of the system (the plant leaf system composed of cells), P is the pressure on the plant cells, V is the volume of the plant cells, U is the test voltage, and Cp is the physiological capacitance of the plant leaves;

[0063] The pressure P on plant cells can be calculated using the pressure formula: Where F is the clamping force, S is the effective area under the action of the plate;

[0064] Model of the change of physiological capacitance Cp of plant leaves with clamping force F:

[0065]

[0066] Assuming that d represents the specific effective thickness of plant leaves, Formula (1) can be transformed into:

[0067]

[0068] make Formula (2) can be transformed into:

[0069] Cp=x0+hF (3)

[0070] Formula (3) is a linear model, where x0 and h are model parameters.

[0071] because therefore

[0072] Since resistive current is caused by dielectric substances, it is determined by factors such as the permeability of the membrane to various dielectric substances and whether the dielectric substances exist in large quantities. External stimuli change the permeability of dielectric substances, affecting the concentration of internal and external dielectric substances. The concentration difference of internal and external dielectric substances obeys the Nernst equation, and the physiological resistance is inversely proportional to the conductivity, while the conductivity is proportional to the concentration of dielectric substances in the cell. From this, it can be deduced that the relationship between the physiological resistance of the cell and external stimuli.

[0073] The amount of water in plant cells is related to the elasticity of plant leaf cells. Under different clamping forces, the permeability of different plant cell membranes changes differently, so their physiological resistance is different.

[0074] The expression of the Nernst equation is as follows:

[0075]

[0076] Where, E is the electromotive force; E 0 is the standard electromotive force; R0 is the ideal gas constant, which is equal to 8.314570 JK -1 .mol -1 , T is temperature, unit K; C i is the concentration of dielectric substances in the cell membrane that responds to physiological resistance, C o is the concentration of dielectric substance outside the cell membrane that responds to physiological resistance, and the total amount of dielectric substance inside and outside the membrane that responds to physiological resistance C T =C i +C o , F0 is Faraday's constant, equal to 96485C.mol -1 ;n R is the number of dielectric substances transferred in response to physiological resistance, in mol.

[0077] The internal energy of the electromotive force E can be converted into pressure work, which is proportional to PV. PV = a E, that is:

[0078]

[0079] Where: P is the pressure on the plant cell, a is the electromotive force conversion energy coefficient, and V is the volume of the plant cell;

[0080] The pressure P on plant cells can be calculated using the pressure formula: Where F is the clamping force, S is the effective area under the action of the plate;

[0081] In mesophyll cells, vacuoles and cytoplasm occupy most of the intracellular space. o With C i The sum is certain, equal to the total amount of dielectric material C inside and outside the membrane that responds to physiological resistanceT , C i It is proportional to the conductivity, and the conductivity is the reciprocal of the resistance R, so, Can be expressed as Where R is the resistance, f0 is the concentration of dielectric substance C in the cell membrane that responds to physiological resistance i The proportional coefficient between the conversion of resistance and the resistance, therefore, equation (5) can be transformed into:

[0082]

[0083] Formula (6) is transformed into

[0084]

[0085] Formula (7) is transformed into

[0086]

[0087] Taking the exponents on both sides of formula (8) can be transformed into:

[0088]

[0089] Further deformation can be obtained:

[0090]

[0091] In formula (10), R is the physiological resistance. Due to the specific effective thickness of plant leaves Formula (10) can be transformed into:

[0092]

[0093] For the same blade under test in the same environment, d, a, E in formula (11) 0 , R0, T, n R , F0, C T , f0 are all constants; let Therefore, formula (11) can be transformed into:

[0094]

[0095] In formula (12), y1, k1 and b1 are the parameters of the model. When F = 0 is substituted into formula (12), the intrinsic physiological resistance IR of plant leaves is obtained: IR = y1 + k1. and Perform deformation operations. Here, E in 0 , R0, T and F0 are fixed values, Only the number n is transferred with the dielectric material that responds to the physiological resistance R Proportional, set Kn R =n R , then: The dielectric material transfer number Kn in response to physiological resistance R The calculation method of Kn is: R =lnk1-lny1; similarly, and Perform deformation calculation to obtain unit metabolic energy based on resistance Metabolic energy ΔG based on resistance R =ΔG R-U d.

[0096] According to Ohm's law, for the physiological current passing through the physiological resistor, the physiological current I R =U / R, where U is the measured voltage; I R is the physiological current passing through the physiological resistor; therefore In the formula, I R To measure the physiological current passing through the physiological resistor with voltage U. Derivative of the above formula, we get: Resistive Material Conductivity ICF R It is defined as I when F = 0 R ′, which is calculated as: According to the derivative formula of the relationship model between physiological resistance and clamping force: Resistive material conduction resistance ICR R It is defined as R' when F = 0, and its calculation method is: ICR R =-b1k1.

[0097] In the impedance measurement of the same object under the same environment, the impedance mainly depends on the concentration of dielectric substances that respond to physiological impedance inside and outside the membrane. Therefore, the permeability of the membrane to various dielectric substances that respond to physiological impedance and the water content determine the cell impedance. For leaves, the impedance depends even more on the concentration of dielectric substances that respond to physiological impedance inside and outside the membrane. External excitation changes the membrane permeability of the dielectric substance, affecting the concentration of dielectric substances that respond to physiological impedance inside and outside the membrane. The concentration difference of dielectric substances that respond to physiological impedance inside and outside the membrane also obeys the Nernst equation. When the concentration of dielectric substances that respond to physiological impedance outside the membrane is constant, the physiological impedance is inversely proportional to the concentration of dielectric substances that respond to physiological impedance inside the cell. It can be deduced that the physiological impedance of the cell is also related to external excitation.

[0098] The amount of water in plant cells is related to the elasticity of plant leaf cells. Under different clamping forces, the permeability of the dielectric substance in response to physiological impedance of different plant cell membranes changes differently, so their physiological impedance is different.

[0099] The expression of the Nernst equation is as follows:

[0100]

[0101] Where E is the electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, equal to 8.314570 JK -1 .mol -1 ; T is temperature, unit K; Q i is the concentration of dielectric substances in the cell membrane that responds to physiological impedance, Q o is the concentration of dielectric substances outside the cell membrane that respond to physiological impedance, and the total amount of dielectric substances inside and outside the membrane that respond to physiological impedance is Q = Q i +Q o , F0 is Faraday's constant, equal to 96485C.mol -1 ;n Z is the number of dielectric substances transferred in response to physiological impedance, in mol.

[0102] The internal energy of the electromotive force E can be converted into pressure work, which is proportional to PV. PV = a E, that is:

[0103]

[0104] Among them: P is also the pressure on the plant cell, a is also the electromotive force conversion energy coefficient, and V is also the volume of the plant cell;

[0105] The pressure P on plant cells can be calculated using the pressure formula: Where F is also the clamping force, and S is the effective area under the action of the plate;

[0106] In mesophyll cells, vacuoles and cytoplasm occupy most of the space in the cell. o With Q i The sum is certain, equal to the total amount of dielectric material Q, Q inside and outside the membrane that responds to physiological impedance i It is proportional to the conductivity of the dielectric material that responds to the physiological impedance, and the conductivity of the dielectric material that responds to the physiological impedance is the inverse of the impedance Z. Therefore, Can be expressed as Z is impedance, J0 is the dielectric concentration Q in the cell membrane that responds to physiological impedance i The proportional coefficient between the impedance and the impedance is thus converted into (14):

[0107]

[0108] Formula (15) is transformed into

[0109]

[0110] Formula (16) can be transformed into:

[0111]

[0112] Taking the exponents on both sides of formula (17), it can be transformed into:

[0113]

[0114] Further deformation can be obtained:

[0115]

[0116] In formula (19), Z is the physiological impedance. Formula (19) can be transformed into:

[0117]

[0118] For the same blade under test in the same environment, d, a, E in formula (20) 0 , R0, T, n Z , F0, Q, J0 are all fixed values, let Therefore, formula (20) can be transformed into:

[0119]

[0120] Where y2, k2 and b2 are the parameters of the model. When F = 0 is substituted into equation (21), the intrinsic physiological impedance IZ of plant leaves is obtained: IZ = y2 + k2; and Perform deformation operations. Here, E in 0 , R0, T and F0 are fixed values, Only with dielectric materials that respond to physiological impedance transfer number n Z Proportional, set Kn Z =n Z , then: The dielectric material transfer number Kn in response to physiological impedance Z The calculation method of Kn is: Z =lnk2-lny2; similarly, and Perform deformation calculation to obtain unit metabolic energy based on impedance Impedance-based metabolic energy ΔG Z =ΔG Z-U d.

[0121] According to Ohm's law, for the physiological current passing through the physiological impedance, the physiological current I Z=U / Z, where U is the measured voltage; I Z is the physiological current through the physiological impedance; therefore In the formula, I Z To measure the physiological current passing through the physiological impedance with voltage U. Derivative of the above formula, we get: Total dielectric conductivity ICF Z It is defined as I when F = 0 Z ′, which is calculated as: According to the derivative formula of the relationship model between physiological impedance and clamping force: Total dielectric conductivity resistance ICR Z It is defined as Z' when F = 0, and its calculation method is: ICR Z =-b2k2.

[0122] In the capacitance measurement of the same object under the same environment, the capacitance mainly depends on the concentration of dielectric substances that respond to physiological capacitance inside and outside the membrane. Therefore, the permeability of the membrane to various dielectric substances that respond to physiological capacitance determines the cell capacitance. For leaves, the capacitance depends on the concentration of dielectric substances that respond to physiological capacitance inside and outside the membrane. External excitation changes the membrane permeability of the dielectric substance, affecting the concentration of dielectric substances that respond to physiological capacitance inside and outside the membrane. The concentration difference of dielectric substances that respond to physiological capacitance inside and outside the membrane also obeys the Nernst equation. When the concentration of dielectric substances that respond to physiological capacitance outside the membrane is constant, the physiological capacitance is inversely proportional to the concentration of dielectric substances that respond to physiological capacitance inside the cell. It can be deduced that the physiological capacitance of the cell is also related to external excitation.

[0123] The amount of water in plant cells is related to the elasticity of plant leaf cells. Under different clamping forces, the permeability of dielectric substances in response to physiological capacitance of different plant cell membranes changes differently, so their physiological capacitance is different.

[0124] The expression of the Nernst equation is as follows:

[0125]

[0126] Where E is the electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, equal to 8.314570 JK -1 .mol -1 ; T is temperature, unit K; X i is the concentration of dielectric substances in the cell membrane that responds to physiological capacitance, X o is the concentration of dielectric substance outside the cell membrane that responds to physiological capacitance, and the total amount of dielectric substance inside and outside the membrane that responds to physiological capacitance is X = X i +X o, F0 is Faraday's constant, equal to 96485C.mol -1 ;n XC It is the number of dielectric substances transferred in response to physiological capacitive reactance, in mol.

[0127] The internal energy of the electromotive force E can be converted into pressure work, which is proportional to PV. PV = a E, that is:

[0128]

[0129] Among them: P is also the pressure on the plant cell, a is also the electromotive force conversion energy coefficient, and V is also the volume of the plant cell;

[0130] The pressure P on plant cells can be calculated using the pressure formula: Where F is also the clamping force, and S is the effective area under the action of the plate;

[0131] In mesophyll cells, vacuoles and cytoplasm occupy most of the space in the cell. o With X i The sum is certain, equal to the total amount of dielectric material X, X inside and outside the membrane that responds to physiological capacitive reactance i It is proportional to the conductivity of the dielectric material that responds to the physiological capacitive reactance, and the conductivity of the dielectric material that responds to the physiological capacitive reactance is the reciprocal of the capacitive reactance Xc. Therefore, Can be expressed as Xc is the capacitive reactance, L0 is the dielectric substance concentration X in the cell membrane that responds to the physiological capacitive reactance i The proportional coefficient between the conversion of and physiological capacitive reactance can be converted into (23):

[0132]

[0133] Formula (24) is transformed into

[0134]

[0135] Formula (25) can be transformed into:

[0136]

[0137] Taking the exponents on both sides of formula (26) can be transformed into:

[0138] Further deformation can be obtained:

[0139]

[0140] In formula (28), Xc is the physiological capacitive reactance. Due to the specific effective thickness of plant leaves Formula (28) can be transformed into:

[0141]

[0142] For the same blade under test in the same environment, d, a, E in formula (29) 0 , R0, T, n XC , F0, X, L0 are all fixed values, let Therefore, formula (29) can be transformed into:

[0143]

[0144] In formula (30), y3, k3 and b3 are parameters of the model. When F=0 is substituted into formula (30), the inherent physiological capacitive reactance IXc of the plant leaf is obtained: IXc=y3+k3; the capacitance converted from the inherent physiological capacitive reactance IXc of the plant leaf is the inherent physiological capacitance ICP. The formula for converting the inherent physiological capacitive reactance into the inherent physiological capacitance is: Where IXc is the inherent physiological capacitive reactance of plant leaves, ICP is the inherent physiological capacitance, f is the test frequency, and π is the ratio of pi, which is equal to 3.1416. and Perform deformation operations. Here, E 0 , R0, T and F0 are fixed values, Only with dielectric materials that respond to physiological capacitive reactance, the number n is transferred XC Proportional, set Kn XC =n XC , then: The dielectric material transfer number Kn in response to physiological capacitive reactance XC The calculation method of Kn is: XC =lnk3-lny3; similarly, and Perform deformation calculation to obtain unit metabolic energy based on capacitive reactance Metabolizable energy ΔG based on capacitive reactance Xc =ΔG Xc-U d.

[0145] According to Ohm's law, for the physiological current passing through the physiological capacitive reactance, the physiological current I Xc =U / Xc, where U is the measured voltage; I Xc is the physiological current passing through the physiological capacitive reactance; therefore In the formula, I Xc To measure the physiological current through the physiological capacitive reactance with voltage U. Derivative the above formula, we get: Capacitive Conductivity ICF C It is defined as I when F = 0 X c′, which is calculated as: According to the derivative formula of the relationship model between physiological capacitive reactance and clamping force: Capacitive material conduction resistance ICR C It is defined as Xc′ when F=0, and its calculation method is: ICR Xc =-b3k3.

[0146] Similarly, under different clamping forces, the permeability of dielectric substances in response to physiological reactance of different plant cell membranes also changes differently, so their physiological reactance is different.

[0147] The expression of the Nernst equation is as follows:

[0148]

[0149] Where E is the electromotive force, E 0 is the standard electromotive force, R0 is the ideal gas constant, equal to 8.314570 JK -1 .mol -1 ; T is temperature, unit K; M i is the concentration of dielectric substances in the cell membrane that respond to physiological reactance, M o is the concentration of dielectric substances outside the cell membrane that respond to physiological reactance, and the total amount of dielectric substances inside and outside the membrane that respond to physiological reactance M T =M i +M o , F0 is Faraday's constant, equal to 96485C.mol -1 ;n XL It is the number of dielectric substances transferred in response to physiological reactance, in mol.

[0150] The internal energy of the electromotive force E can be converted into pressure work, which is proportional to PV. PV = a E, that is:

[0151]

[0152] Among them: P is also the pressure on the plant cell, a is also the electromotive force conversion energy coefficient, and V is also the volume of the plant cell;

[0153] The pressure P on plant cells can be calculated using the pressure formula: Where F is also the clamping force, and S is the effective area under the action of the plate;

[0154] In mesophyll cells, vacuoles and cytoplasm occupy most of the intracellular space. o With M i The sum is certain, equal to the total amount of dielectric material M that responds to physiological reactance inside and outside the membrane T , M iIt is proportional to the conductivity of the dielectric material that responds to the physiological inductive reactance, and the conductivity of the dielectric material that responds to the physiological inductive reactance is the reciprocal of the inductive reactance Xl. Therefore, Can be expressed as Xl is the inductive reactance, P0 is the dielectric substance concentration M in the cell membrane that responds to the physiological inductive reactance i The proportional coefficient between the conversion of and inductive reactance is, therefore, equation (32) can be transformed into:

[0155]

[0156] Formula (33) is transformed into

[0157]

[0158] Formula (34) can be transformed into:

[0159]

[0160] Taking the exponents on both sides of formula (35) can be transformed into:

[0161]

[0162] Further deformation can be obtained:

[0163]

[0164] In formula (37), Xl is the physiological inductive reactance. Formula (37) can be transformed into:

[0165]

[0166] For the same blade under test in the same environment, d, a, E in formula (38) 0 , R0, T, n XL ,F0,M T , P0 are all fixed values, let Therefore, formula (38) can be transformed into:

[0167]

[0168] In formula (39), y4, k4 and b4 are the parameters of the model. When F = 0 is substituted into formula (39), the inherent physiological resistance of plant leaves IXl is obtained: IXl = y4 + k4; and Perform deformation operations. Here, E 0 , R0, T and F0 are fixed values, Only the number n is transferred to the dielectric material that responds to physiological inductanceXL Proportional, set Kn XL =n XL , then: The dielectric material transfer number Kn in response to physiological reactance XL The calculation method of Kn is: XL =lnk4-lny4; similarly, and Perform deformation calculation to obtain unit metabolic energy based on inductive reactance Metabolizable energy ΔG based on inductive reactance Xl =ΔG Xl-U d.

[0169] According to Ohm's law, for the physiological current passing through the physiological inductance, the physiological current I Xl =U / Xl, where U is the measured voltage; I Xl is the physiological current passing through the physiological inductance; therefore In the formula, I Xl To measure the physiological current passing through the physiological inductive reactance with voltage U. Derivative of the above formula: Capacitive Conductivity ICF L It is defined as I when F = 0 L ′, which is calculated as: According to the derivative formula of the relationship model between physiological inductive reactance and clamping force: Capacitive material conduction resistance ICR L It is defined as Xl' when F = 0, and its calculation method is: ICR L =-b4k4.

[0170] Since cells (organelles) are spherical structures, the growth of cells is closely related to the growth of their volume. For the same plant organ, especially leaves, the volume of cells is positively correlated with the volume of their vacuoles, and the main component of vacuoles is water. The capacitance of plant cells can be calculated using the formula of concentric spherical capacitors:

[0171]

[0172] Here, π is the ratio of the circumference of a circle to 3.1416, C is the capacitance of the concentric spherical capacitor, ε is the dielectric constant of the electrolyte, and R1 and R2 are the radii of the outer and inner spheres, respectively. In cells (organelles), R2-R1 can be used as the thickness of the membrane, R1≈R2, and the same type of cells (organelles) in the same plant tissue and organ have a certain membrane thickness and ε, so the volume of the cell (organelle) has the following relationship with the capacitance C of the cell:

[0173]

[0174] In formula (41), the same type of cell (organelle) of the same plant tissue and organ is constant, and because the volume of cells (organelles), especially those of unfolded leaves, is proportional to the water holding capacity, that is, the water holding capacity of cells is proportional to is proportional to, so we can use Characterizing the water holding capacity of plant leaves, the method for calculating the intracellular water holding capacity IWHC based on the inherent physiological capacitance ICP is:

[0175] The specific effective thickness d of plant leaves represents the growth of cells. The intracellular water holding capacity IWHC supports the growth of plant cells d, which can be characterized as the leaf intracellular water use efficiency IWUE, and the calculation method is:

[0176] According to Ohm's law, for the physiological current passing through the physiological impedance, the physiological current I Z =U / Z, where U is the measured voltage, I Z is the physiological current. Z is the impedance; at the same time, the current is equal to the capacitance multiplied by the differential of the voltage in time. After integral transformation, the time t is the product of the capacitance and the impedance. Therefore, based on the inherent physiological capacitance ICP and the inherent physiological impedance IZ of the plant leaves, the calculation formula for the intracellular water holding time IWHT of the plant based on electrophysiological parameters is: IWHT=ICP×IZ. Based on the intracellular water holding capacity IWHC and the intracellular water holding time IWHT, the intracellular water transfer rate IWTR can be calculated. Its calculation formula is: The intracellular nutrient transfer rate INTR and the intracellular water transfer rate IWTR are similar in concept and have the same values.

[0177] The calculation formula of the plant's intrinsic physiological resistance IR is: Among them, IR1, IR2, IR3, ...IR n is the intrinsic resistance of each unit cell membrane. Assuming that the intrinsic resistance of each unit cell membrane is equal, that is, IR1=IR2=IR3=...=IR n =IR0, then the calculation formula of the plant's inherent physiological resistance is: Where n can be characterized as the number of proteins and lipids that cause electrical resistance in biological tissues.

[0178] The calculation formula of the plant's inherent physiological resistivity IXc is: Among them, IXc1, IXc2, IXc3, ...IXc p is the intrinsic capacitive reactance of each unit cell membrane. Assuming that the intrinsic capacitive reactance of each unit cell membrane is equal, that is, IXc1 = IXc2 = IXc3 = ... = IXc p =IXc0, then the calculation formula of the plant's inherent physiological capacitive reactance is: Among them, p can be characterized as the number of proteins that cause the resistance of biological tissues, especially surface proteins (peripheral proteins).

[0179] The calculation formula of plant's inherent physiological resistance IXl is: Among them, IXl1, IXl2, IXl3, ... IXl q is the intrinsic inductive reactance of each unit cell membrane. Assuming that the intrinsic inductive reactance of each unit cell membrane is equal, that is, IXl1 = IXl2 = IXl3 = ... = IXl q = IXl0, then the calculation formula of the plant's inherent physiological resistance is: Where q can be characterized as the number of protein-binding proteins (intrinsic proteins) that cause resistance in biological tissues, especially the number of transport proteins therein.

[0180] The intrinsic physiological resistance of plant leaves is IXl - The calculation formula is: Plant leaf intrinsic physiological resistance inverse IXc - The calculation formula is: The inverse of the intrinsic physiological resistance of plant leaves R - The calculation formula is: The ratio of the cell's material transport capacity caused by surface proteins (peripheral proteins) to the total material transport capacity determines the passive transport capacity of nutrients, while the ratio of the cell's material transport capacity caused by bound proteins to the total material transport capacity determines the active transport capacity of nutrients. At the same time, due to the same measurement leaf Therefore, the calculation formula of intracellular nutrient active transport capacity NAC is: NAC = UAF × INTR. Passive transport unit flow Since the same measurement leaf The calculation formula of intracellular nutrient active transport capacity NPC is: NPC = UPF × INTR. The calculation formula of intracellular nutrient transport capacity NTC is: NTC = UNF × INTR. Since the active transport capacity of plants determines the minimum concentration of ion absorption, it also determines the low nutrient tolerance of plants. Therefore, the low nutrient tolerance of plants can be expressed as the proportion of active transport capacity of plants to the total nutrient transport capacity of plants. The plant nutrient utilization efficiency is expressed as

[0181] Metabolic capacity is related to bioelectric current. The greater the bioelectric current, the more complete the metabolism. Therefore, the bioelectric current I based on the inherent physiological resistance is R =U / IR, biocurrent I based on inherent physiological capacitive reactanceXc =U / IXc, bioelectric current I based on inherent physiological inductance Xl =U / IXl and biocurrent I based on intrinsic physiological impedance IZ =U / IZ. Here, U represents the voltage applied during measurement. Since it is a parallel connection, U is the same. Therefore, we can define the plant leaf metabolic flow MF as:

[0182] The active transport of nutrients is related to the metabolic rate. The stronger the active transport capacity of nutrients, the greater the metabolic rate. Therefore, we define the metabolic rate of plant leaves MR: MR = INTR × NAC.

[0183] According to the plant leaf metabolic flux MF and the plant leaf metabolic rate MR, the relative metabolic activity MA of the plant based on electrophysiological information is redefined:

[0184] Example 1

[0185] Bletilla striata variety Guiji No. 1, grown in bare land, was selected at Puding Karst Ecological Comprehensive Experimental Station of Institute of Geochemistry, Chinese Academy of Sciences. The leaves were clamped between parallel electrode plates of the measuring device, and the measuring voltage was set to 1.5V and the frequency was 3000Hz. The physiological capacitance, physiological resistance and physiological impedance of the Bletilla striata leaves under different clamping forces of the first, second, third, fourth and fifth unfolded leaves were measured in parallel mode, and the physiological capacitive reactance and physiological inductive reactance of the Bletilla striata leaves were calculated. The models of physiological capacitance, physiological resistance, physiological impedance, physiological capacitive reactance and physiological inductive reactance of each Bletilla striata leaf to be tested with clamping force were constructed respectively. The parameters of each model were obtained. According to the parameters of each model of each leaf, the inherent physiological resistance, inherent physiological impedance and physiological inductive reactance of the Bletilla striata leaves were calculated. The metabolic activity of Bletilla striata was obtained by measuring the physical impedance, inherent physiological capacitance, inherent physiological inductance and specific effective thickness, as well as the intracellular water metabolism, intracellular nutrient metabolism, operation of various dielectric substances in the cell, and energy metabolism of various dielectric substances in the cell. The intracellular water metabolism of Bletilla striata includes the intracellular water holding capacity IWHC, the intracellular water utilization efficiency IWUE, the intracellular water holding time IWHT and the intracellular water transfer rate IWTR. The intracellular nutrient metabolism of Bletilla striata includes the intracellular nutrient transfer rate INTR, the intracellular nutrient utilization efficiency NUE, the low nutrient tolerance RLN, the active transport unit flow UAF, the intracellular nutrient active transport capacity NAC, and the intracellular nutrient transport capacity NTC. The operation of various dielectric substances in the cell includes the dielectric substance transfer number Kn that responds to the physiological resistance. R , Dielectric material transfer number Kn in response to physiological capacitive reactance XC , Dielectric material transfer number Kn in response to physiological reactance XL and the dielectric mass transfer number Kn in response to physiological impedance Z, resistive material conductivity ICF R , resistive material conduction resistance ICR R , Capacitive Material Conductivity ICF C , Capacitive material conduction resistance ICR C , Inductive Material Conductivity ICF L , Inductive material conduction resistance ICR L , total dielectric conductivity ICF Z and total dielectric conductivity resistance ICR Z ; Energy metabolism of various dielectric substances in cells, including unit metabolic energy ΔG based on resistance R-U and the metabolic energy ΔG based on resistance R , Unit metabolic energy ΔG based on impedance Z-U and impedance-based metabolic energy ΔG Z , Unit metabolic energy ΔG based on capacitive reactance Xc-U and metabolic energy ΔG based on capacitive reactance Xc , Unit metabolic energy ΔG based on inductive reactance Xl-U and the metabolic energy ΔG based on inductive resistance Xl ; The metabolic activity of Bletilla striata includes the metabolic flow MF of Bletilla striata leaves, the metabolic rate MR of Bletilla striata leaves and the relative metabolic activity MA of Bletilla striata.

[0186] The data obtained above are formed into a physical examination report as shown in Table 2.

[0187] Table 2 Physical examination report of Guiji No. 1

[0188]

[0189]

[0190] The biological significance and physiological function significance of each indicator in the above physical examination report are shown in Table 1.

[0191] The values ​​of intracellular water metabolism of Guiji No. 1 leaves were calibrated on each leaf to be tested, and the intracellular water metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular water metabolism function of each leaf were given, as shown in Table 3.

[0192] Table 3 Differences and subdivision ranking of water metabolism functions in leaves of Guiji No. 1

[0193]

[0194] The values ​​of intracellular nutrient metabolism of Guiji No. 1 leaves were calibrated on each leaf to be tested, and the intracellular nutrient metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular nutrient metabolism function of each leaf were given as shown in Table 4.

[0195] Table 4 Differences and subdivision ranking of intracellular nutrient metabolism functions in leaves of Guiji No. 1

[0196]

[0197] The numerical values ​​of the operation of various dielectric substances in the cells of Guiji No. 1 leaves were calibrated on each leaf to be tested, and the operation functions of various dielectric substances in the cells of each leaf were subdivided and ranked, and the differences in the operation functions of various dielectric substances in the cells of each leaf were given as shown in Table 5.

[0198] Table 5 Differences and subdivisions of the operational functions of various dielectric substances in the cells of each leaf of Guiji No. 1

[0199]

[0200]

[0201] The values ​​of energy metabolism of various dielectric substances in the cells of Guiji No. 1 leaves were calibrated on each leaf to be tested, and the energy metabolism functions of various dielectric substances in the cells of each leaf were subdivided and ranked, and the differences in energy metabolism functions of various dielectric substances in the cells of each leaf were given as shown in Table 6.

[0202] Table 6 Differences and subdivisions in the energy metabolism of various dielectric substances in the cells of each leaf of Guiji No. 1

[0203]

[0204] The values ​​of the metabolic activity of Guiji No. 1 leaves were calibrated on each leaf to be tested, and the metabolic activity of each leaf was subdivided and ranked, and the differences in the overall metabolic functions of each leaf were given as shown in Table 7.

[0205] Table 7 Differences and subdivision rankings of metabolic activities of leaves of Guiji No. 1

[0206]

[0207] According to the differences in the intracellular water metabolism function, intracellular nutrient metabolism function, intracellular dielectric substance operation function, intracellular dielectric substance energy metabolism function and plant overall metabolism function of each leaf of Guiji No. 1, it can be seen that the fifth unfolded leaf of Guiji No. 1 is stronger than other leaves in all aspects. This is because it is closest to the root. The growth and development of the tuber of Bletilla striata requires a large amount of energy and organic matter from the fifth unfolded leaf. Its function is of great significance to the growth and development of Bletilla striata and the formation of yield. The function of the first unfolded leaf and the second unfolded leaf is weaker than that of other leaves. This is related to the fact that the height of the Bletilla striata plant is basically stable at this time, and only less substances and energy are needed to maintain its life activities and functions.

[0208] Example 2

[0209] The implementation location and method are the same as those in Example 1. The difference is that the test plant is Bletilla striata in the karst rock habitat, and the variety is Rocky striata No. 1. The physical examination report of Rocky striata No. 1 is shown in Table 8.

[0210] Table 8 Physical examination report of Yanji No. 1 (~ indicates missing data)

[0211]

[0212]

[0213] The biological significance and physiological function significance of each indicator in the above physical examination report are the same as those in Example 1, as shown in Table 2.

[0214] The numerical values ​​of intracellular water metabolism of Yanji No. 1 leaves were calibrated on each leaf to be tested, and the intracellular water metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular water metabolism function of each leaf were given, as shown in Table 9.

[0215] Table 9 Differences and subdivision ranking of water metabolism functions in the leaves of Yanji No. 1

[0216]

[0217] The numerical values ​​of intracellular nutrient metabolism of Yanji No. 1 leaves were calibrated on each leaf to be tested, and the intracellular nutrient metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular nutrient metabolism function of each leaf were given as shown in Table 10.

[0218] Table 10 Differences and subdivision ranking of intracellular nutrient metabolism functions of each leaf of Yanji No. 1

[0219]

[0220] The numerical values ​​of the operation of various dielectric substances in the cells of Yanji No. 1 leaves were calibrated on each leaf to be tested, and the operation functions of various dielectric substances in the cells of each leaf were subdivided and ranked, and the differences in the operation functions of various dielectric substances in the cells of each leaf were given as shown in Table 11.

[0221] Table 11 Differences and subdivisions of the operating functions of various dielectric substances in the cells of each leaf of Yanji No. 1 (~ indicates missing data)

[0222]

[0223] The numerical values ​​of energy metabolism of various dielectric substances in the cells of Yanji No. 1 leaves were calibrated on each leaf to be tested, and the energy metabolism functions of various dielectric substances in the cells of each leaf were subdivided and ranked, and the differences in energy metabolism functions of various dielectric substances in the cells of each leaf were given as shown in Table 12.

[0224] Table 12 Differences and subdivisions of energy metabolism of various dielectric substances in the cells of each leaf of Yanji No. 1 (~ indicates missing data)

[0225]

[0226] The values ​​of the metabolic activity of the leaves of Yanji No. 1 were calibrated on each leaf to be tested, and the metabolic activity of each leaf was subdivided and ranked, and the differences in the overall metabolic functions of each leaf were given as shown in Table 13.

[0227] Table 13 Differences and subdivision rankings of metabolic activity of each leaf of Yanji No. 1

[0228]

[0229] In addition to the energy metabolism function of various dielectric substances in the cell (due to the lack of data), according to the differences in the intracellular water metabolism function, intracellular nutrient metabolism function, the operation function of various dielectric substances in the cell and the overall metabolic function of the plant among the leaves of Yanji No. 1, it can be seen that the third and first unfolded leaves of Yanji No. 1 are stronger than the other three leaves in all aspects. This shows that Yanji No. 1 strengthens the functions of the third and first unfolded leaves, which provides a basis for Bletilla striata to adapt to the karst environment (karst drought, high pH, ​​high bicarbonate) at any time. The function of the second unfolded leaf is weaker than that of other leaves, which is also related to the fact that the height of Bletilla striata is basically stable at this time, and only less substances and energy are needed to maintain its life activities and functions. Yanji No. 1 strengthens the function of the third unfolded leaf, which plays a dual role. On the one hand, it increases the karst adaptability of Bletilla striata, and on the other hand, like the fifth unfolded leaf of Guiji No. 1, it provides a large amount of energy and organic matter for the growth and development of Bletilla striata tubers. Therefore, the strength of the third expanded leaf function of Yanji No. 1 is of great significance to the karst adaptability and yield formation of Bletilla striata.

[0230] Example 3

[0231] The implementation location, site conditions and methods used are the same as those of Example 1. The difference is that the tested variety is Flathead No. 1. The physical examination report of Flathead No. 1 is shown in Table 14.

[0232] Table 14 Physical Examination Report of Flathead No. 1

[0233]

[0234]

[0235] The biological significance and physiological function significance of each indicator in the above physical examination report are the same as those in Example 1, as shown in Table 2.

[0236] The numerical values ​​of intracellular water metabolism of Pingtou No. 1 leaves were calibrated on each leaf to be tested, and the intracellular water metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular water metabolism function of each leaf were given, as shown in Table 15.

[0237] Table 15 Differences and subdivisions of water metabolism functions in leaves of Pingtou No. 1

[0238]

[0239] The numerical values ​​of intracellular nutrient metabolism of Pingtou No. 1 leaves were calibrated on each leaf to be tested, and the intracellular nutrient metabolism function of each leaf was subdivided and ranked, and the differences in the intracellular nutrient metabolism function of each leaf were given as shown in Table 16.

[0240] Table 16 Differences and subdivisions of the intracellular nutrient metabolism functions of each leaf of Pingtou No. 1

[0241]

[0242] The numerical values ​​of the operation of various dielectric substances in the cells of Flat Head No. 1 blade are calibrated on each blade to be tested, and the operation functions of various dielectric substances in the cells of each blade are subdivided and ranked, and the differences in the operation functions of various dielectric substances in the cells of each blade are given as shown in Table 17.

[0243] Table 17 Differences and subdivisions of the operating functions of various dielectric substances in the leaf cells of Pingtou No. 1

[0244]

[0245] The numerical values ​​of energy metabolism of various dielectric substances in the cells of Pingtou No. 1 leaves were calibrated on each leaf to be tested, and the energy metabolism functions of various dielectric substances in the cells of each leaf were subdivided and ranked, and the differences in energy metabolism functions of various dielectric substances in the cells of each leaf were given as shown in Table 18.

[0246] Table 18 Differences and subdivisions in the energy metabolism of various dielectric substances in the cells of each leaf of Pingtou No. 1

[0247] First expanded leaf Second expanded leaf The third leaf Fourth leaf <![CDATA[Resistance-based specific metabolic energy ΔG R-U > 2.010 2.000 1.470 2.880 <![CDATA[Resistance-based metabolic energy ΔG R > 33.550 23.630 12.690 53.340 <![CDATA[Impedance-based specific metabolic energy ΔG Z-U > 3.740 2.680 1.760 2.840 <![CDATA[Impedance-based metabolic energy ΔG Z > 62.390 31.560 15.160 52.590 <![CDATA[ΔG of unit metabolic energy based on capacitance reactance Xc-U > 4.070 2.880 1.770 10.830 <![CDATA[Metabolic energy ΔG based on capacitance reactance Xc > 67.80 33.98 15.27 200.18 <![CDATA[Unit metabolic energy ΔG based on inductive reactance Xl-U > 2.360 1.970 1.520 2.800 <![CDATA[Metabolic energy ΔG based on inductive reactance Xl > 39.280 23.170 13.080 51.810 Energy metabolism middle weak Very weak middle Sorting 2 3 4 1

[0248] The numerical value of the metabolic activity of Pingtou No. 1 leaves was calibrated on each leaf to be tested, and the metabolic activity of each leaf was subdivided and ranked, and the differences in the overall metabolic functions of each leaf were given as shown in Table 19.

[0249] Table 19 Differences and subdivision rankings of metabolic activities of each leaf of Pingtou No. 1

[0250]

[0251] In addition to the energy metabolism function of various dielectric substances in the cell, according to the differences in the intracellular water metabolism function, intracellular nutrient metabolism function, the operation function of various dielectric substances in the cell and the overall metabolic function of the plant in each leaf of Pingtou No. 1, it can be seen that the third unfolded leaf of Pingtou No. 1 is stronger than the other three leaves in all aspects. Although the third unfolded leaf of Bletilla striata Pingtou No. 1 has a strong physiological function, it is significantly different from the third unfolded leaf of Yanji No. 1. Its energy metabolism is weaker than the other three leaves, which limits its role in the environmental adaptability of Bletilla striata and its growth, development and yield formation. Therefore, it often forms ineffective seedlings in production and it is difficult to achieve high yield. At the same time, the third unfolded leaf of Pingtou No. 1 is not enough to support the needs of the other three leaves (sink) when used as a water and nutrient source, while Yanji No. 1 has two water and nutrient sources, which are enough to support the needs of the other three leaves (sink). The water and nutrient supply of the fifth unfolded leaf of Guiji No. 1 is also equal to or even exceeds the two water and nutrient sources of Yanji No. 1. This is the physiological basis for it to support the growth and development of the other four leaves. These results showed that Guiji No. 1 had excellent high-yield characteristics, Yanji No. 1 had the comprehensive characteristics of high yield and adaptability, while the specialized third expanded leaf of Pingtou No. 1 was unable to provide sufficient organic matter and energy for tuber growth due to the uncoordinated source-sink relationship and mismatched energy metabolism, which was the physiological reason for the formation of ineffective seedlings of Pingtou No. 1; this was consistent with production practice.

Claims

1. A method for rapid quantification of differences in plant leaf physiological functions, It is characterized in that The following steps are involved: Step 1, quickly measuring the physiological capacitance, physiological resistance and physiological impedance of each leaf of the plant to be tested under different clamping forces and calculating the physiological capacitive reactance and physiological inductive reactance of the plant leaves; Construct models of the physiological capacitance, physiological resistance, physiological impedance, physiological capacitive reactance, and physiological inductive reactance of each leaf to be tested of the plant respectively as a function of the clamping force; and obtain the parameters of each model; Step 2: Calculate the intrinsic physiological resistance, intrinsic physiological impedance, intrinsic physiological capacitance, intrinsic physiological inductance and specific effective thickness of the plant leaves, as well as the intracellular water metabolism, intracellular nutrient metabolism, intracellular operation of various dielectric substances, and intracellular energy metabolism of various dielectric substances, using the parameters of each model of each leaf to be tested in step 1, and then obtain the metabolic vitality of the plant, wherein the intracellular operation of various dielectric substances includes the dielectric substance transfer number Kn in response to the physiological resistance. R , Dielectric material transfer number Kn in response to physiological capacitive reactance XC , Dielectric material transfer number Kn in response to physiological reactance XL and the dielectric mass transfer number Kn in response to physiological impedance Z , resistive material conductivity ICF R , resistive material conduction resistance ICR R , Capacitive Material Conductivity ICF C , Capacitive material conduction resistance ICR C , Inductive Material Conductivity ICF L , Inductive material conduction resistance ICR L , total dielectric conductivity ICF Z and total dielectric conductivity resistance ICR Z ; Step 3, forming a physical examination report with the data calculated in step 2; Step 4: Give the biological and physiological significance of each indicator in the physical examination report; Step five, calibrate the physiological function values ​​of the leaves on each leaf to be tested, rank the functions and functional rankings of each leaf, and diagnose the significance and role of the specialized physiological function leaves on plant growth and development and yield formation.

2. A method for rapidly quantifying differences in plant leaf physiological functions according to claim 1, Features: In the step 1, the physiological resistance of the plant leaf changes with the clamping force model, Where R is the physiological resistance, E is the electromotive force, and E 0 is the standard electromotive force, R 0 is the ideal gas constant, T is the temperature, C i is the concentration of dielectric substances in the cell membrane that responds to physiological resistance, C o is the concentration of dielectric substance outside the cell membrane that responds to physiological resistance, f 0 is the concentration of dielectric substance C in the cell membrane that responds to physiological resistance i The proportional coefficient between the conversion and physiological resistance, the total amount of dielectric material C inside and outside the membrane that responds to physiological resistance T =C i +C o , F 0 is the Faraday constant, n R is the number of dielectric substances transferred in response to physiological resistance; E can be used to do work and is proportional to PV. PV = aE, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological resistance of plant leaves changing with the clamping force can be transformed into where y 1 , k 1 and b 1 is the parameter of the model, and the calculation method of the plant leaf intrinsic physiological resistance IR is: IR = y 1 +k 1 .

3. A method for rapidly quantifying differences in plant leaf physiological functions according to claim 12, Features: In the step 1, the physiological impedance of the plant leaf changes with the clamping force model, Where Z is physiological impedance, E is electromotive force, E 0 is the standard electromotive force, R 0 is the ideal gas constant, T is the temperature, Q i is the concentration of dielectric substances in the cell membrane that responds to physiological impedance, Q o is the dielectric substance concentration outside the cell membrane that responds to physiological impedance, J 0 is the dielectric substance concentration Q in the cell membrane that responds to physiological impedance i The proportional coefficient between the conversion and impedance, the total amount of dielectric material inside and outside the membrane that responds to physiological impedance Q = Q i +Q o , F 0 is the Faraday constant, n Z is the number of dielectric substances transferred in response to physiological impedance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological impedance of plant leaves changing with the clamping force can be transformed into where y 2 , k 2 and b 2 is the parameter of the model, and the calculation method of the plant leaf intrinsic physiological impedance IZ is: IZ = y 2 +k 2 .

4. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 3, Features: In the step 1, the model of the physiological capacitive reactance of the plant leaf changing with the clamping force, Where Xc is the physiological capacitive reactance, E is the electromotive force, E 0 is the standard electromotive force, R 0 is the ideal gas constant, T is the temperature, X i is the concentration of dielectric substances in the cell membrane that responds to physiological capacitance, X o is the dielectric substance concentration outside the cell membrane that responds to physiological capacitance, L 0 is the dielectric substance concentration X in the cell membrane that responds to physiological capacitance i The proportional coefficient between the conversion and physiological capacitance, the total amount of dielectric material inside and outside the membrane that responds to physiological capacitance X = X i +X o , F 0 is the Faraday constant, n XC is the number of dielectric material transfers in response to physiological capacitive reactance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of the plant cell, P is the pressure on the plant cell, and the pressure P is given by the pressure formula Calculate, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf; let The model of the physiological capacitive reactance of plant leaves changing with the clamping force can be transformed into where y 3 , k 3 and b 3 is the parameter of the model, and the calculation method of the inherent physiological capacitive reactance IXc of the plant leaf is: IXc = y 3 +k 3 .

5. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 4, Features: In the step 1, the model of the physiological inductive reactance of the plant leaves changing with the clamping force, Where Xl is the physiological inductive reactance, E is the electromotive force, E 0 is the standard electromotive force, R 0 is the ideal gas constant, T is the temperature, M i is the concentration of dielectric substances in the cell membrane that respond to physiological reactance, M o is the dielectric substance concentration outside the cell membrane that responds to physiological inductance, P 0 is the dielectric substance concentration M in the cell membrane that responds to physiological reactance i The proportional coefficient between the conversion and physiological inductance, the total amount of dielectric material M inside and outside the membrane that responds to physiological inductance T =M i +M o , F 0 is the Faraday constant, n XL is the number of dielectric substances transferred in response to physiological inductive reactance; E can be used to do work and is proportional to PV. PV = a E, a is the coefficient of converting electromotive force into metabolic energy, V is the volume of plant cells, P is the pressure on plant cells, and pressure P is given by the pressure formula Find out, F is the clamping force, S is the effective area under the action of the plate, d is the specific effective thickness of the plant leaf, let The model of the physiological inductive reactance of plant leaves changing with the clamping force can be transformed into where y 4 , k 4 and b 4 is the parameter of the model, and the calculation method of the inherent physiological resistance IXl of the plant leaves is: IXl = y 4 +k 4 .

6. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 5, Features: In the step 2, the dielectric material transfer number Kn of the response physiological resistance R The calculation method of Kn is: R =lnk 1 -lny 1 ; Dielectric material transfer number Kn in response to physiological capacitive reactance XC The calculation method of Kn is: XC =lnk 3 -lny 3 ; Dielectric material transfer number Kn in response to physiological reactance XL The calculation method of Kn is: XL =lnk 4 -lny 4 ; Dielectric material transfer number Kn in response to physiological impedance Z The calculation method of Kn is: Z =lnk 2 -lny 2 ; Resistive material conductivity ICF R The calculation method is: Resistive material conduction resistance ICR R The calculation method is: ICR R =-b 1 k 1 , Capacitive Material Conductivity ICF C The calculation method is: Capacitive material conduction resistance ICR C The calculation method is: ICR C =-b 3 k 3 , Inductive Material Conductivity ICF L The calculation method is: Inductive material conduction resistance ICR L The calculation method is: ICR L =-b 4 k 4 ; Total dielectric conductivity ICF Z The calculation method is: Total dielectric conductivity resistance ICR Z The calculation method is: ICR Z =-b 2 k 2 .

7. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 5, Features: In step 2, the energy metabolism of various dielectric substances in the cell includes the unit metabolic energy ΔG based on resistance. R-U and the metabolic energy ΔG based on resistance R , Unit metabolic energy ΔG based on impedance Z-U and impedance-based metabolic energy ΔG Z , Unit metabolic energy ΔG based on capacitive reactance Xc-U and metabolic energy ΔG based on capacitive reactance Xc , Unit metabolic energy ΔG based on inductive reactance Xl-U and the metabolic energy ΔG based on inductive resistance Xl ; Unit metabolic energy ΔG based on resistance R-U The calculation method is: Metabolic energy ΔG based on resistance R The calculation method is: ΔG R =ΔG R-U d; Unit metabolic energy ΔG based on impedance Z-U The calculation method is: Impedance-based metabolic energy ΔG Z The calculation method is: ΔG Z =ΔG Z-U d; Unit metabolic energy ΔG based on capacitive reactance Xc-U The calculation method is: Metabolizable energy ΔG based on capacitive reactance Xc The calculation method is: ΔG Xc =ΔG Xc-U d; Unit metabolic energy ΔG based on inductive reactance Xl-U The calculation method is: Metabolizable energy ΔG based on inductive reactance Xl The calculation method is: ΔG Xl =ΔG Xl-U d.

8. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 1, Features: In step 2, the calculation method of the plant leaf specific effective thickness d is: Wherein U is the test voltage, and h is the parameter of the model of the physiological capacitance of the plant leaf changing with the clamping force.

9. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 1, Features: In the step 2, the intracellular nutrient metabolism of the plant includes the intracellular nutrient transfer rate INTR, the intracellular nutrient utilization efficiency NUE, the low nutrient tolerance capacity RLN, the active transport unit flow UAF, the intracellular nutrient active transport capacity NAC, and the intracellular nutrient transport capacity NTC; the intracellular nutrient transfer rate INTR and the intracellular water transfer rate IWTR are similar in concept and have the same value; the calculation formula of the intracellular nutrient utilization efficiency NUE is: The calculation formula for low nutrition tolerance RLN is: The calculation formula of active transport unit flow UAF is: The calculation formula of intracellular nutrient active transport capacity NAC is: NAC = UAF × INTR; the calculation formula of intracellular nutrient transport capacity NTC is: NTC = UNF × INTR, where UNF is the nutrient unit flow, which can be expressed as:

10. A method for rapid quantitative analysis of differences in plant leaf physiological functions according to claim 1, Features: The step five specifically includes: S501, calibrating the values ​​of leaf intracellular water metabolism on each leaf to be tested, subdividing and ranking the intracellular water metabolism functions of each leaf, and giving the differences in the intracellular water metabolism functions of each leaf; S502, calibrating the values ​​of leaf intracellular nutrient metabolism on each leaf to be tested, subdividing and ranking the intracellular nutrient metabolism functions of each leaf, and giving the differences in the intracellular nutrient metabolism functions of each leaf; S503, calibrating the values ​​of the operation of various dielectric substances in the leaf cells on each leaf to be tested, subdividing and ranking the operation functions of various dielectric substances in the cells of each leaf, and giving the differences in the operation functions of various dielectric substances in the cells of each leaf; S504, calibrating the values ​​of energy metabolism of various dielectric substances in leaf cells on each leaf to be tested, subdividing and ranking the energy metabolism functions of various dielectric substances in cells of each leaf, and giving the differences in energy metabolism functions of various dielectric substances in cells of each leaf; S505, calibrating the value of the leaf metabolic activity on each leaf to be tested, subdividing and ranking the metabolic activity of each leaf, and providing the difference of the overall metabolic function of each leaf; S506, based on the differences among leaves in intracellular water metabolism function, intracellular nutrient metabolism function, intracellular various dielectric substance operation function, intracellular various dielectric substance energy metabolism function and overall plant metabolism function.

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