Organic fertilizer for increasing sugar content of grape fruits and preparation method thereof

Abstract

The invention discloses an organic fertilizer for increasing the sugar content of grape fruits and a preparation method thereof.The organic fertilizer is prepared from, by weight, 30-40 parts of biomass, 5-15 parts of plant ash, 5-10 parts of urea, 20-30 parts of decomposed livestock and poultry manure, 15-20 parts of calcium magnesium phosphate fertilizer, 10-15 parts of potassium fertilizer, 25-35 parts of modified microecological preparation and 1-3 parts of microelements; wherein the modified microecological preparation is prepared from a modified carrier and a complex microbial inoculant. The organic fertilizer has the effects of improving soil quality, increasing the content of organic matters in soil and promoting growth of grape roots, so that absorption of grapes to nutritional ingredients in the soil is improved, the utilization rate of the organic fertilizer is increased, the growth requirements of the grapes are met, and the sugar content and yield of grape fruits are increased.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizers, and in particular relates to an organic fertilizer for increasing the sugar content of grape fruits and a preparation method thereof. Background Art

[0002] Grapes are deciduous vines of the genus Vitis. The berries are mostly round or oval, and the color varies with the variety. Grapes are one of the main fruits in my country and are increasingly favored by people in modern life. The application of fertilizers not only affects the yield of grapes, but also affects their quality. The most important indicator of the taste quality of fresh grapes is the sugar content of the berries.

[0003] The main factors affecting glucose content include varieties, climate, soil conditions, fertilizer and water management, hormone regulation, etc. Varieties with good quality can be selected during planting. At present, climate conditions are still uncontrollable. Hormone regulation has obvious effects, but improper use will be counterproductive. The most adjustable method is soil, fertilizer and water management. Through fertilization, irrigation and other methods, the physical and chemical conditions of the soil are improved, and the grape growth environment is improved to improve the quality and yield of grapes. During the growth and development process, grapes need to absorb nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, iron, boron, zinc, manganese, molybdenum, copper and other nutrients from the soil. Among the various nutrients in the soil, nitrogen, phosphorus and potassium are the nutrients that grapes need the most for growth, except carbon, oxygen and hydrogen, and are also the nutrients that are taken away the most when harvested. However, the amount of these nutrients returned to the soil in the form of stubble and roots is the least. In order to change this situation, it is necessary to supplement nitrogen, phosphorus and potassium to the soil through fertilization for plant absorption and utilization.

[0004] Organic fertilizers are mainly composed of organic carbon, nitrogen, phosphorus, potassium and a variety of trace elements. As a major agricultural country, my country has a long history of using organic fertilizers. The application of organic fertilizers can significantly increase soil organic matter, which is an important substance for improving soil, thereby improving the quality of grapes.

[0005] Chinese patent CN104086317A discloses an organic ecological fertilizer for grapes and its preparation method, which is mainly prepared from the following raw materials in parts by weight: 71.9-92.1 parts of inorganic salt fertilizer, 8-12 parts of grape slag, 1-4 parts of antibiotic fertilizer, 1-4 parts of water retaining agent, 0.1-0.5 parts of agricultural amino acid, 0.3-0.9 parts of boric acid, and 0.04-0.15 parts of brassinolide. The organic ecological fertilizer for grapes of the invention provides the most adequate nutrient supplement for grape fields, has the best effect on the quality and yield of grapes, produces large and full grapes, tastes sweet, and has a large yield; can enhance the cold resistance, drought resistance, and disease resistance of grapes, and has a good ecological effect; the special fertilizer made by the fertilizer is stable and reliable in quality, is conducive to the full absorption of various nutrients by grapes, and is conducive to a substantial increase in grape yield, with an increase rate of up to 31.34%-42.54%. However, the organic ecological fertilizer of the invention mainly increases the yield of grapes, and whether it increases the sugar content of grape fruits remains to be discussed. Chinese patent CN113896593A discloses a fertilizer for increasing the sugar content of tangerines and a preparation method thereof, comprising the following components by mass: 100-200 parts of nitrogen fertilizer masterbatch, 20-40 parts of humic acid, 3-10 parts of nano-diatomite, 10-20 parts of silicon dioxide, 0.1-2 parts of inhibitor, and 2-10 parts of stabilizer. The invention improves the utilization rate of fertilizers, improves soil structure, and increases the sugar content of tangerines; humic acid is a macromolecule with aromatic rings and alicyclic rings as the basic structure, and the rings are connected with functional groups such as carboxyl, hydroxyl, carbonyl, quinone, and methoxy. The ion exchange and chelation of humic acid can significantly improve the utilization rate of fertilizers. At the same time, humic acid ion exchange can help soil agglomerate into a particle structure, thereby improving porosity and air permeability; using nitric acid with a certain oxidizing property to activate lignite can oxidize the macromolecular lignite humic acid into small molecular lignite humic acid, which can improve the cation exchange capacity. However, the fertilizer in this patent is applied to the sugar content of tangerines, and whether it is also applicable to grapes needs further research.

[0006] At present, there are few reports on organic fertilizers for increasing the sugar content of grape fruits. Therefore, it is of great significance to develop an organic fertilizer for increasing the sugar content of grape fruits in this field. Summary of the invention

[0007] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an organic fertilizer for increasing the sugar content of grape fruit and a preparation method thereof. The organic fertilizer has the functions of improving soil quality, increasing the organic matter content in the soil, and promoting the growth of grape roots, thereby improving the absorption of nutrients in the soil by grapes and improving the utilization rate of organic fertilizers, which not only meets the growth needs of grapes, but also increases the sugar content and yield of grape fruit.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials by weight:

[0010] 30-40 parts of biomass, 5-15 parts of plant ash, 5-10 parts of urea, 40-50 parts of decomposed livestock and poultry manure, 15-20 parts of calcium magnesium phosphate fertilizer, 10-15 parts of potash fertilizer, 25-35 parts of modified microecological preparations, and 1-3 parts of trace elements;

[0011] Wherein, the modified microecological preparation is made of a modified carrier and a composite bacterial agent.

[0012] In the present invention, the added wood ash helps to improve the physical structure of the soil, increase the air permeability and water permeability of the soil, and can also promote the formation of soil aggregate structure, reduce leaching and loss of nutrients, and can improve the effect of organic fertilizers and the overall quality of the soil; the added urea contains high concentrations of nitrogen, which can be used as a source of nitrogen fertilizer, thereby quickly replenishing the nitrogen element in the soil, helping to promote the development of the grape root system and promote plant growth; the added calcium magnesium phosphate fertilizer not only provides a variety of necessary nutrients, but also improves the soil structure and regulates the soil pH, providing necessary nutrients for soil microorganisms, phosphorus promotes root growth and development, and calcium helps root tip growth and root hair development, thereby improving the yield and quality of grapes.

[0013] Preferably, an organic fertilizer for increasing the sugar content of grape fruit comprises the following raw materials by weight:

[0014] 35-40 parts of biomass, 10-15 parts of wood ash, 7-10 parts of urea, 45-50 parts of decomposed livestock and poultry manure, 18-20 parts of calcium magnesium phosphate fertilizer, 10-13 parts of potassium fertilizer, 30-35 parts of modified microecological preparations, and 1-2 parts of trace elements.

[0015] Preferably, the biomass is one or more of corn straw powder, sorghum straw powder, wheat straw powder, and rice straw powder; the potash fertilizer is one or more of potassium dihydrogen phosphate, potassium sulfate, and potassium chloride; and the trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate, and sodium molybdate in a mass ratio of 6-8:3-5:2-3:3-4:4-5:1-2.

[0016] In the present invention, the added biomass is straw powder, which releases macroelements such as nitrogen, phosphorus, and potassium after decomposition, provides trace elements such as zinc, iron, and manganese, and can also improve the soil environment, increase the soil's water holding capacity, increase the organic matter in the soil, provide a stable growth environment, slowly release nutrients, and meet the long-term needs of plants.

[0017] Preferably, the method for preparing the decomposed livestock and poultry manure is as follows:

[0018] By weight, 60-80 parts of pig manure, 30-40 parts of cow manure and 10-20 parts of chicken manure are mixed evenly, water is added to the mixture until the moisture content reaches 60-70%, and then 7-12 parts of a decomposing agent are added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0019] In the present invention, the added decomposed livestock and poultry manure can not only provide rich nutrients, but also improve the soil structure and promote the healthy development of the soil ecosystem. It contains a large number of elements such as nitrogen, phosphorus, potassium, etc., and can also provide trace elements such as iron, zinc, and manganese. The nutrients are released slowly and for a long time. It can also provide nutrients and energy for soil microorganisms, increase the diversity of soil microorganisms, accelerate the decomposition and mineralization of organic matter, and enhance the stability of the soil ecosystem.

[0020] Preferably, the method for preparing the modified microecological preparation comprises the following steps:

[0021] S1. Porous biochar: Coconut shell powder is added to deionized water, followed by sodium dodecylbenzene sulfonate, zinc chloride and ammonia water, and a hydrothermal carbonization reaction is carried out. After the reaction is completed, the solid product is filtered, and a nitric acid solution is added. The solid product is ultrasonically treated at room temperature, and after the treatment, the solid product is filtered and dried to obtain porous biochar.

[0022] S2, modified porous biochar: adding the porous biochar prepared in step S1 to an ethanol aqueous solution, then adding vinyl triethoxysilane, stirring for reaction, filtering, washing, and drying after the reaction is completed to obtain vinyl porous biochar; then adding the vinyl porous biochar to DMF, then adding acrylic acid, acrylamide, and ammonium persulfate, heating for reaction, filtering, washing, and drying after the reaction is completed to obtain modified porous biochar;

[0023] S3, modified carrier: add β-cyclodextrin to deionized water, adjust the pH to 11-12, then add p-toluenesulfonyl chloride DMF solution, react at 0-4°C for 4-6h, add the modified porous biochar in step S2 after the reaction is completed, and react at a constant temperature. After the reaction is completed, filter, wash and dry to obtain a modified carrier;

[0024] S4, modified microecological preparation: adding the modified carrier, composite bacterial agent and naphthyl acetic acid solution in step S3 into deionized water, stirring and immersing in a dark environment, filtering and drying after immersion, and obtaining the modified microecological preparation.

[0025] Preferably, in step S1, the mass fraction of the ammonia water is 10-20%, the mass ratio of the coconut shell powder, deionized water, sodium dodecylbenzene sulfonate, zinc chloride and ammonia water is 60-80:800-1000:4-8:10-20:50-80, the temperature of the hydrothermal carbonization reaction is 200-240°C, and the time is 4-7h; the mass concentration of nitric acid is 10-15%, the power of the ultrasonic treatment is 150-200W, and the time is 20-30min.

[0026] In the present invention, the modified micro-state preparation is added with porous biochar as a carrier, and the porous biochar is prepared by hydrothermal carbonization of biomass coconut shell. Sodium dodecylbenzene sulfonate, zinc chloride and ammonia water are added while preparing the biochar. The added sodium dodecylbenzene sulfonate is used as a surfactant to improve the dispersibility of zinc ions, and zinc hydroxide is generated in situ on the biochar, which may promote the pore formation of the biochar during the pyrolysis process and increase the specific surface area of ​​the biochar. The pyrolyzed biochar is reacted with nitric acid to remove zinc oxide, thereby further improving the pore structure of the porous biochar.

[0027] Preferably, in step S2, the mass ratio of the porous biochar and vinyltriethoxysilane is 50-60:5-9, the mass fraction of ethanol in the ethanol aqueous solution is 70-80%, the temperature of the stirring reaction is 60-70°C, and the time is 1-2h; the mass ratio of the vinyl porous biochar, acrylic acid, acrylamide, and ammonium persulfate is 50-60:20-30:30-40:0.4-0.8, the temperature of the heating reaction is 70-80°C, and the time is 3-5h.

[0028] In the present invention, porous biochar is reacted with vinyltriethoxysilane to introduce active double bond groups on the porous biochar, and then acrylic acid and acrylamide are introduced on the porous biochar through free radical reaction to improve the hydrophilicity and water retention properties of the porous biochar. At the same time, the introduction of a large number of amino groups can also improve the adsorption and subsequent reactivity of the porous biochar.

[0029] Preferably, in step S3, the mass ratio of the β-cyclodextrin, deionized water, toluenesulfonyl chloride DMF solution, and modified porous biochar is 10-20:700-900:100-150:50-60, the mass fraction of the toluenesulfonyl chloride DMF solution is 10-20%, the temperature of the isothermal reaction is 80-90°C, and the time is 4-8h.

[0030] In the present invention, β-cyclodextrin is reacted with p-toluenesulfonyl chloride, and the hydroxyl group on the β-cyclodextrin reacts with the sulfonyl chloride group of p-toluenesulfonyl chloride, thereby introducing toluenesulfonyl groups into the β-cyclodextrin, which is beneficial to the reaction of β-cyclodextrin with modified porous biochar. Subsequently, the modified porous biochar is reacted with the β-cyclodextrin introduced with toluenesulfonyl groups, thereby introducing β-cyclodextrin into the porous biochar, thereby obtaining a modified carrier.

[0031] Preferably, in step S4, the composite bacterial agent is a mixture of Bacillus gellingii, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 3-6:2-4:1-3; the mass fraction of the naphthaleneacetic acid solution is 5-10%, the mass ratio of the modified carrier, the composite bacterial agent, the naphthaleneacetic acid solution, and deionized water is 50:5-10:20-30:400-500, the stirring and impregnation speed is 30-50r / min, the temperature is 30-40°C, the time is 1-2h, and the drying temperature is 50-60°C.

[0032] In the present invention, the modified carrier is stirred and adsorbed with the composite bacterial agent and naphthaleneacetic acid, so that the composite bacterial agent and naphthaleneacetic acid are loaded on the modified carrier. The beta-cyclodextrin introduced on the modified carrier not only improves the adsorption of the composite bacterial agent by the porous biochar, but also the hydrophobic cavity structure in the beta-cyclodextrin can encapsulate the naphthaleneacetic acid. After the obtained modified microecological preparation is used as an organic fertilizer, the naphthaleneacetic acid can be slowly released during the growth of grapes, so as to promote the growth of grape roots, thereby improving the adsorption of nutrients by the grape roots and increasing the sugar content of the grape fruit.

[0033] Specifically, in the composite bacterial agent added by the present invention, Bacillus gellingii, Aspergillus oryzae, and Streptomyces tenuifolius can decompose organic matter in the soil with high accuracy, release nutrients, and promote nutrient circulation. They can also secrete extracellular polysaccharides to form a stable aggregate structure in the soil. At the same time, they can also participate in the construction of soil microbial communities, maintain the stability of the ecosystem, and promote the benign interaction between plants, soil, and microorganisms, thereby improving the growth of grapes and increasing the sugar content of grape fruits.

[0034] The present invention also protects a method for preparing the organic fertilizer for increasing the sugar content of grape fruit as described above, comprising the following steps:

[0035] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium fertilizer and trace elements are mixed, stirred for 20-30 minutes, and then biomass, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 10-20 minutes to obtain the organic fertilizer.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] (1) The organic fertilizer for increasing the sugar content of grape fruit provided by the present invention is compounded with a plurality of specific raw materials, thereby ensuring the nutrient requirements of grapes during growth. The raw materials cooperate with each other to promote the effective absorption of nutrients by the grape root system, thereby improving the utilization rate of the organic fertilizer by the grapes, promoting the photosynthesis of the grapes, improving the photosynthetic efficiency, and accelerating the synthesis and transportation of sugar during the growth of the grapes. At the same time, the organic fertilizer is rich in organic matter and microorganisms, which helps to improve the soil structure and soil fertility, promote the root system to absorb nutrients, and thus increase the sugar content of the grape fruit.

[0038] (2) The organic fertilizer for increasing the sugar content of grape fruit provided by the present invention is prepared by adding a homemade modified microecological preparation and using biomass coconut shell as raw material to obtain porous biochar. The mineral elements rich in the porous biochar have a positive effect on the soil, not only increasing the nutrient content in the soil, but also activating the nutrient elements. The porous biochar has a strong adsorption effect on nitrogen salts and phosphates in the soil, which can effectively reduce the loss of nutrient elements and fix them in the soil, thereby facilitating the adsorption of grape roots, thereby promoting the growth of grapes and increasing the sugar content of grape fruits. Subsequently, the hydrophilic water-retaining copolymer acrylic acid-acrylamide is introduced into the porous biochar through a free radical reaction. The acrylamide contains a large number of amide groups, which not only improves the adsorption of the porous biochar, but also improves the water and fertilizer retention of the organic fertilizer. Then, β-cyclodextrin is introduced into the modified porous biochar through chemical grafting. Finally, the composite bacterial agent and naphthaleneacetic acid are loaded on the modified carrier. β-cyclodextrin improves the poor water solubility of naphthaleneacetic acid, so that it can be slowly released in the subsequent process and absorbed by the root system of grapes, thereby promoting the growth of roots, avoiding the loss caused by the poor water solubility of naphthaleneacetic acid, and improving the utilization rate of naphthaleneacetic acid. The modified microecological preparation can affect the soil nutrient cycle and utilization by affecting the types, quantity and activity of microorganisms in the soil. Its porous characteristics and surface characteristics can provide a safe habitat for microorganisms in the soil and reduce the survival competition among microorganisms. In addition, the modified ecological preparation can also affect the adsorption and release of nutrients by the soil. The porous biochar can store carbon sources in the soil and become a source bank of organic matter in the soil. It can also promote the polymerization of small organic molecules to form soil organic matter through surface activation, thereby changing the carbon nutrient cycle in the soil and increasing the sugar content in grape grains. DETAILED DESCRIPTION

[0039] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] The decomposition agent was purchased from Weifang Yihao Biotechnology Co., Ltd., and the effective viable bacteria count was ≥ 2×10 10 cfu / g; the calcium magnesium phosphate fertilizer was purchased from Jinan Dewen Chemical Co., Ltd., with an effective phosphorus content of ≥12%; the gelling Bacillus was purchased from Weifang Yihao Biotechnology Co., Ltd., with an effective viable count of ≥1.0×10 10 cfu / g; the Aspergillus oryzae was purchased from Shandong Infida Biotechnology Co., Ltd., with an effective viable count of ≥5.0×10 10 cfu / g; the Streptomyces leucoderma was purchased from Shandong Nuojie Biotechnology Co., Ltd., and the effective viable count was ≥1.0×10 10 cfu / g;

[0041] Example 1

[0042] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0043] 3.5kg corn stalk powder, 1kg plant ash, 0.8kg urea, 4.5kg decomposed livestock and poultry manure, 1.8kg calcium magnesium phosphate fertilizer, 1.3kg potassium dihydrogen phosphate, 3kg modified microecological preparation, 0.2kg trace elements;

[0044] The trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 7:4:2.5:3.5:4.5:1.5.

[0045] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0046] By weight, 7 kg of pig manure, 3.5 kg of cow manure and 1.5 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 65%, and then 1 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0047] The preparation method of the modified microecological preparation comprises the following steps:

[0048] S1, porous biochar: 70g coconut shell powder was added to 900g deionized water, followed by 6g sodium dodecylbenzene sulfonate, 15g zinc chloride, and 70g ammonia water with a mass fraction of 15%, and hydrothermally carbonized at 220°C for 6h. After the reaction was completed, the solid product was filtered, and added to a nitric acid solution with a mass concentration of 15%, and ultrasonically treated at room temperature. The power of the ultrasonic treatment was 180W and the time was 25min. After the treatment was completed, the porous biochar was filtered and dried to obtain the porous biochar;

[0049] S2, modified porous biochar: 55g of the porous biochar in step S1 was added to 800mL of ethanol aqueous solution (the mass fraction of ethanol was 75%), and then 7g of vinyl triethoxysilane was added, and the mixture was stirred and reacted at 65°C for 1.5h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain vinyl porous biochar; then 55g of the vinyl porous biochar was added to 1L of DMF, and then 25g of acrylic acid, 35g of acrylamide, and 0.6g of ammonium persulfate were added, and the mixture was reacted at 75°C for 4h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain modified porous biochar;

[0050] S3, modified carrier: 15g of β-cyclodextrin was added to 800g of deionized water, and a 5% NaOH solution was added to adjust the pH to 12, and then 130g of a 15% p-toluenesulfonyl chloride DMF solution was added, and the reaction was carried out at 2°C for 5h. After the reaction was completed, 55g of the modified porous biochar in step S2 was added, and the reaction was carried out at a constant temperature of 85°C for 6h. After the reaction was completed, the modified carrier was filtered, washed, and dried to obtain the modified carrier;

[0051] S4, modified probiotic preparation: add 50g of the modified carrier in step S3, 8g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 5:3:2), and 25g of 8% naphthaleneacetic acid ethanol solution into 450g of deionized water, stir and immerse for 1.5h at a speed of 40r / min and a temperature of 35°C in a dark environment, filter after immersion, and dry at 55°C to obtain the modified probiotic preparation.

[0052] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0053] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium dihydrogen phosphate, and trace elements are mixed, stirred for 25 minutes, and then corn straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 15 minutes to obtain the organic fertilizer.

[0054] Example 2

[0055] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0056] 3.5kg sorghum straw powder, 1kg wood ash, 0.7kg urea, 4.5kg decomposed livestock and poultry manure, 1.7kg calcium magnesium phosphate fertilizer, 1.2kg potassium sulfate, 3kg modified microecological preparation, 0.2kg trace elements;

[0057] The trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 7:4:2.5:3.5:4.5:1.5.

[0058] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0059] By weight, 7 kg of pig manure, 3.5 kg of cow manure and 1.5 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 65%, and then 0.9 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0060] The preparation method of the modified microecological preparation comprises the following steps:

[0061] S1. Porous biochar: 70 g coconut shell powder was added to 900 g deionized water, followed by 6 g sodium dodecylbenzene sulfonate, 15 g zinc chloride, and 60 g ammonia water with a mass fraction of 15%, and hydrothermally carbonized at 220 ° C for 5 h. After the reaction was completed, the solid product was added to a nitric acid solution with a mass concentration of 10%, and ultrasonically treated at room temperature. The power of the ultrasonic treatment was 170 W and the time was 25 min. After the treatment was completed, the porous biochar was filtered and dried to obtain the porous biochar.

[0062] S2, modified porous biochar: 55g of the porous biochar in step S1 was added to 800mL of ethanol aqueous solution (the mass fraction of ethanol was 75%), and then 7g of vinyl triethoxysilane was added, and the mixture was stirred and reacted at 65°C for 1.5h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain vinyl porous biochar; then 55g of the vinyl porous biochar was added to 1L of DMF, and then 25g of acrylic acid, 35g of acrylamide, and 0.6g of ammonium persulfate were added, and the mixture was reacted at 75°C for 4h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain modified porous biochar;

[0063] S3, modified carrier: 15g of β-cyclodextrin was added to 800g of deionized water, and a 5% NaOH solution was added to adjust the pH to 11, and then 120g of a 15% p-toluenesulfonyl chloride DMF solution was added, and the reaction was carried out at 2°C for 5h. After the reaction was completed, 55g of the modified porous biochar in step S2 was added, and the reaction was carried out at a constant temperature of 85°C for 6h. After the reaction was completed, the modified carrier was filtered, washed, and dried to obtain a modified carrier;

[0064] S4, modified probiotic preparation: add 50g of the modified carrier in step S3, 7g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 4:3:3), and 25g of 8% naphthaleneacetic acid ethanol solution into 450g of deionized water, stir and immerse for 1.5h at a speed of 40r / min and a temperature of 35°C under light-proof conditions, filter after immersion, and dry at 55°C to obtain the modified probiotic preparation.

[0065] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0066] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium sulfate and trace elements are mixed, stirred for 25 minutes, and then sorghum straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 15 minutes to obtain the organic fertilizer.

[0067] Example 3

[0068] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0069] 3kg wheat straw powder, 0.5kg wood ash, 0.5kg urea, 4kg decomposed livestock and poultry manure, 1.5kg calcium magnesium phosphate fertilizer, 1kg potassium chloride, 2.5kg modified microecological preparation, 0.1kg trace elements;

[0070] Wherein, the trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 6:3:2:3:4:1.

[0071] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0072] By weight, 6 kg of pig manure, 3 kg of cow manure and 1 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 60%, and then 0.7 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0073] The preparation method of the modified microecological preparation comprises the following steps:

[0074] S1. Porous biochar: 60 g coconut shell powder was added to 800 g deionized water, followed by 4 g sodium dodecylbenzene sulfonate, 10 g zinc chloride, and 50 g ammonia water with a mass fraction of 20%, and hydrothermally carbonized at 200 ° C for 7 h. After the reaction was completed, the solid product was added to a nitric acid solution with a mass concentration of 10%, and ultrasonically treated at room temperature. The power of the ultrasonic treatment was 150 W and the time was 30 min. After the treatment was completed, it was filtered and dried to obtain porous biochar;

[0075] S2, modified porous biochar: add 50g of the porous biochar in step S1 to 800mL of ethanol aqueous solution (the mass fraction of ethanol is 70%), then add 5g of vinyl triethoxysilane, stir and react at 60°C for 2h, filter, wash and dry after the reaction is completed to obtain vinyl porous biochar; then add 50g of vinyl porous biochar to 1L of DMF, then add 20g of acrylic acid, 30g of acrylamide and 0.4g of ammonium persulfate, react at 70°C for 5h, filter, wash and dry after the reaction is completed to obtain modified porous biochar;

[0076] S3, modified carrier: add 10g of β-cyclodextrin to 700g of deionized water, add 5% NaOH solution to adjust the pH to 11, then add 100g of 10% p-toluenesulfonyl chloride DMF solution, react at 0°C for 6h, add 50g of modified porous biochar in step S2 after the reaction is completed, react at 80°C for 8h, filter, wash and dry after the reaction is completed to obtain a modified carrier;

[0077] S4, modified probiotic preparation: add 50g of the modified carrier in step S3, 5g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 3:2:1), and 20g of a 5% ethanol solution of naphthaleneacetic acid to 400g of deionized water, stir and immerse for 2h at a speed of 30r / min and a temperature of 30°C under light-proof conditions, filter after immersion, and dry at 50°C to obtain the modified probiotic preparation.

[0078] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0079] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium chloride and trace elements are mixed, and stirred for 20 minutes, and then wheat straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 10 minutes to obtain the organic fertilizer.

[0080] Example 4

[0081] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0082] 4kg rice straw powder, 1.5kg wood ash, 1kg urea, 5kg decomposed livestock and poultry manure, 2kg calcium magnesium phosphate fertilizer, 1.5kg potassium dihydrogen phosphate, 0.5kg modified microecological preparation, 0.3kg trace elements;

[0083] The trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 8:5:3:4:5:2.

[0084] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0085] By weight, 8 kg of pig manure, 4 kg of cow manure and 2 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 70%, and then 1.2 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0086] The preparation method of the modified microecological preparation comprises the following steps:

[0087] S1. Porous biochar: 80 g of coconut shell powder was added to 1000 g of deionized water, followed by 8 g of sodium dodecylbenzene sulfonate, 20 g of zinc chloride, and 80 g of 10% ammonia water, and the mixture was hydrothermally carbonized at 40°C for 4 h. After the reaction was completed, the mixture was filtered, and the solid product was added to a 5% nitric acid solution, and subjected to ultrasonic treatment at room temperature. The power of the ultrasonic treatment was 200 W, and the time was 20 min. After the treatment, the mixture was filtered and dried to obtain porous biochar.

[0088] S2, modified porous biochar: add 60g of the porous biochar in step S1 to 800mL of ethanol aqueous solution (the mass fraction of ethanol is 80%), then add 9g of vinyl triethoxysilane, stir and react at 70°C for 1h, filter, wash and dry after the reaction is completed to obtain vinyl porous biochar; then add 60g of vinyl porous biochar to 1L of DMF, then add 30g of acrylic acid, 40g of acrylamide and 0.8g of ammonium persulfate, react at 80°C for 3h, filter, wash and dry after the reaction is completed to obtain modified porous biochar;

[0089] S3, modified carrier: 20g of β-cyclodextrin was added to 900g of deionized water, and a 5% NaOH solution was added to adjust the pH to 12, and then 150g of a 20% p-toluenesulfonyl chloride DMF solution was added, and the mixture was reacted at 4°C for 4h. After the reaction was completed, 60g of the modified porous biochar in step S2 was added, and the mixture was reacted at a constant temperature of 90°C for 4h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain a modified carrier;

[0090] S4, modified probiotic preparation: add 50g of the modified carrier in step S3, 10g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 6:4:3), and 30g of a 10% naphthaleneacetic acid ethanol solution to 500g of deionized water, stir and immerse for 1h at a speed of 50r / min and a temperature of 40°C under light-proof conditions, filter after immersion, and dry at 60°C to obtain the modified probiotic preparation.

[0091] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0092] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium dihydrogen phosphate and trace elements are mixed, stirred for 30 minutes, and then rice straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 20 minutes to obtain the organic fertilizer.

[0093] Comparative Example 1

[0094] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0095] 3.5kg corn stalk powder, 1kg plant ash, 0.8kg urea, 4.5kg decomposed livestock and poultry manure, 1.8kg calcium magnesium phosphate fertilizer, 1.3kg potassium dihydrogen phosphate, 3kg modified microecological preparation, 0.2kg trace elements;

[0096] The trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 7:4:2.5:3.5:4.5:1.5.

[0097] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0098] By weight, 7 kg of pig manure, 3.5 kg of cow manure and 1.5 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 65%, and then 1 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0099] The preparation method of the modified microecological preparation comprises the following steps:

[0100] S1, porous biochar: 70g coconut shell powder was added to 900g deionized water, followed by 6g sodium dodecylbenzene sulfonate, 15g zinc chloride, and 70g ammonia water with a mass fraction of 15%, and hydrothermally carbonized at 220°C for 6h. After the reaction was completed, the solid product was filtered, and added to a nitric acid solution with a mass concentration of 15%, and ultrasonically treated at room temperature. The power of the ultrasonic treatment was 180W and the time was 25min. After the treatment was completed, the porous biochar was filtered and dried to obtain the porous biochar;

[0101] S2, modified porous biochar: 55g of the porous biochar in step S1 was added to 800mL of ethanol aqueous solution (the mass fraction of ethanol was 75%), and then 7g of vinyl triethoxysilane was added, and the mixture was stirred and reacted at 65°C for 1.5h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain vinyl porous biochar; then 55g of the vinyl porous biochar was added to 1L of DMF, and then 25g of acrylic acid, 35g of acrylamide, and 0.6g of ammonium persulfate were added, and the mixture was reacted at 75°C for 4h. After the reaction was completed, the mixture was filtered, washed, and dried to obtain modified porous biochar;

[0102] S3, modified microecological preparation: add 50g of the modified porous biochar in step S2, 8g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 5:3:2), and 25g of 8% naphthaleneacetic acid ethanol solution into 450g of deionized water, stir and immerse for 1.5h at a speed of 40r / min and a temperature of 35°C under light-proof conditions, filter after immersion, and dry at 55°C to obtain the modified microecological preparation.

[0103] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0104] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium dihydrogen phosphate, and trace elements are mixed, stirred for 25 minutes, and then corn straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 15 minutes to obtain the organic fertilizer.

[0105] Comparative Example 2

[0106] An organic fertilizer for increasing the sugar content of grape fruit, comprising the following raw materials:

[0107] 3.5kg corn stalk powder, 1kg plant ash, 0.8kg urea, 4.5kg decomposed livestock and poultry manure, 1.8kg calcium magnesium phosphate fertilizer, 1.3kg potassium dihydrogen phosphate, 3kg modified microecological preparation, 0.2kg trace elements;

[0108] The trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate and sodium molybdate in a mass ratio of 7:4:2.5:3.5:4.5:1.5.

[0109] The preparation method of the decomposed livestock and poultry excrement is as follows:

[0110] By weight, 7 kg of pig manure, 3.5 kg of cow manure and 1.5 kg of chicken manure are mixed evenly, water is added to the mixture until the moisture content is 65%, and then 1 kg of a composting agent is added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

[0111] The preparation method of the modified microecological preparation comprises the following steps:

[0112] S1, porous biochar: 70g coconut shell powder was added to 900g deionized water, followed by 6g sodium dodecylbenzene sulfonate, 15g zinc chloride, and 70g ammonia water with a mass fraction of 15%, and hydrothermally carbonized at 220°C for 6h. After the reaction was completed, the solid product was filtered, and added to a nitric acid solution with a mass concentration of 15%, and ultrasonically treated at room temperature. The power of the ultrasonic treatment was 180W and the time was 25min. After the treatment was completed, the porous biochar was filtered and dried to obtain the porous biochar;

[0113] S2, modified probiotic preparation: add 50g of the porous biochar in step S3, 8g of the composite bacterial agent (Bacillus solani, Aspergillus oryzae, and Streptomyces tenuifolius in a mass ratio of 5:3:2), and 25g of 8% naphthaleneacetic acid ethanol solution into 450g of deionized water, stir and immerse for 1.5h at a speed of 40r / min and a temperature of 35°C under light-proof conditions, filter after immersion, and dry at 55°C to obtain the modified probiotic preparation.

[0114] A method for preparing an organic fertilizer for increasing the sugar content of grape fruit comprises the following steps:

[0115] The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium dihydrogen phosphate, and trace elements are mixed, stirred for 25 minutes, and then corn straw powder, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 15 minutes to obtain the organic fertilizer.

[0116] The organic fertilizers prepared in Examples 1-4 and Comparative Examples 1-2 were applied to grape planting, and the specific design was as follows:

[0117] The experimental grape variety was a five-year-old "Hutai No. 8" grape, which was divided into a blank group and a treatment group. Treatment groups 1-4 were respectively applied with the organic fertilizers prepared in Examples 1-4, and treatment groups 5-6 were respectively applied with the organic fertilizers prepared in Comparative Examples 1-2. The blank group was not fertilized, and fertilizers were applied during the flowering period (50 kg / mu) and the swelling period (50 kg / mu). During the growth period, the orchard was managed according to routine procedures, and no fertilizer was applied.

[0118] Soil from the treatment group was collected during the fruit ripening period. A soil drill was used to obtain soil samples from 20-40 cm below the ground surface. 1 kg of soil samples were collected by quartering method and dried in the shade for testing. High-temperature external heating potassium dichromate oxidation-volumetric method, Kjeldahl distillation method, 0.5 mol / LNaHCO3 extraction-molybdenum antimony colorimetric method, and 1.0 mol / LNH4OAc extraction-flame photometry were used to determine soil organic matter, total nitrogen, available phosphorus, and available potassium. The test results are shown in Table 1.

[0119] After the fruit matures, the weight of a single fruit is measured: 30 intact and undamaged fruits from the upper, middle and lower parts of the bunch are taken, weighed with an electronic balance (0.1 g), the readings are recorded, repeated three times, and the average value is calculated; the total soluble sugar in the grape fruit is determined by the anthrone concentrated sulfuric acid colorimetric method; the titratable acid in the grape fruit is determined by the NaOH titration method; yield: during the harvest period, grape bunches with intact bunch shape and consistent maturity are randomly selected from the treatment group to determine the bunch weight, 6 bunches in a group, repeated three times, and the average is taken as the single bunch weight; the same method is used to count the number of bunches per plant in other treatment groups, 6 plants in a group, repeated three times, and the average is taken as the number of bunches per plant; the yield per plant is calculated and converted into total yield per mu; the test results are shown in Table 2.

[0120] Table 1 Effects of organic fertilizers on soil physical and chemical properties

[0121] Organic matter g / kg Total nitrogen g / kg Available phosphorus mg / kg Available potassium mg / kg Blank Group 21.8 1.22 50.78 227.5 Treatment Group 1 33.5 1.64 83.16 346.1 Treatment Group 2 32.7 1.58 81.52 331.5 Treatment Group 3 30.9 1.51 78.91 325.3 Treatment Group 4 33.2 1.62 82.25 332.4 Treatment Group 5 27.6 1.45 71.83 302.5 Treatment Group 6 26.3 1.37 68.47 289.2

[0122] From Table 1, it can be seen that the organic fertilizer prepared by the present invention can significantly increase the organic content of the soil, and the content of both total nutrients and available nutrients in the soil is increased, which is beneficial to the growth of grapes.

[0123] Table 2 Effects of organic fertilizers on grape fruit

[0124]

[0125]

[0126] It can be seen from Table 2 above that the organic fertilizer prepared by the present invention can significantly increase the sugar content of grape fruit, reduce the acidity of grapes, increase the weight of single grapes, and improve the quality and yield of grapes.

[0127] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An organic fertilizer for increasing the sugar content of grape fruit, characterized in that: By weight, it includes the following raw materials: 30-40 parts of biomass, 5-15 parts of plant ash, 5-10 parts of urea, 40-50 parts of decomposed livestock and poultry manure, 15-20 parts of calcium magnesium phosphate fertilizer, 10-15 parts of potash fertilizer, 25-35 parts of modified microecological preparations, and 1-3 parts of trace elements; Wherein, the modified microecological preparation is made of a modified carrier and a composite bacterial agent.

2. The organic fertilizer for increasing the sugar content of grape fruit according to claim 1, characterized in that: By weight, it includes the following raw materials: 35-40 parts of biomass, 10-15 parts of wood ash, 7-10 parts of urea, 45-50 parts of decomposed livestock and poultry manure, 18-20 parts of calcium magnesium phosphate fertilizer, 10-13 parts of potassium fertilizer, 30-35 parts of modified microecological preparations, and 1-2 parts of trace elements.

3. The organic fertilizer for increasing the sugar content of grape fruit according to claim 1, characterized in that: The biomass is one or more of corn straw powder, sorghum straw powder, wheat straw powder, and rice straw powder; the potash fertilizer is one or more of potassium dihydrogen phosphate, potassium sulfate, and potassium chloride; and the trace elements are composed of iron sulfate, borax, manganese sulfate, zinc sulfate, copper sulfate, and sodium molybdate in a mass ratio of 6-8:3-5:2-3:3-4:4-5:1-2.

4. The organic fertilizer for increasing the sugar content of grape fruit according to claim 1, characterized in that: The preparation method of the decomposed livestock and poultry excrement is as follows: By weight, 60-80 parts of pig manure, 30-40 parts of cow manure and 10-20 parts of chicken manure are mixed evenly, water is added to the mixture until the moisture content reaches 60-70%, and then 7-12 parts of a decomposing agent are added. After stirring evenly, the mixture is composted and fermented under ventilation conditions, and the compost is turned over every 2-3 days until the moisture content of the material is ≤30%, thereby obtaining the decomposed livestock and poultry manure.

5. The organic fertilizer for increasing the sugar content of grape fruit according to claim 1, characterized in that: The preparation method of the modified microecological preparation comprises the following steps: S1. Add coconut shell powder to deionized water, then add sodium dodecylbenzene sulfonate, zinc chloride and ammonia water to carry out hydrothermal carbonization reaction. After the reaction is completed, filter the solid product, add nitric acid solution, and ultrasonically treat it at room temperature. After the treatment is completed, filter and dry to obtain porous biochar; S2, adding the porous biochar in step S1 to an ethanol aqueous solution, then adding vinyl triethoxysilane, stirring to react, filtering, washing, and drying after the reaction is completed to obtain vinyl porous biochar; then adding the vinyl porous biochar to DMF, then adding acrylic acid, acrylamide, and ammonium persulfate, heating to react, filtering, washing, and drying after the reaction is completed to obtain modified porous biochar; S3, adding β-cyclodextrin to deionized water, adjusting the pH to 11-12, then adding p-toluenesulfonyl chloride DMF solution, reacting at 0-4°C for 4-6h, adding the modified porous biochar in step S2 after the reaction is completed, and reacting at a constant temperature, filtering, washing, and drying after the reaction is completed to obtain a modified carrier; S4, adding the modified carrier, composite bacterial agent and naphthyl acetic acid solution in step S3 into deionized water, stirring and immersing in a light-proof condition, filtering and drying after immersion, and obtaining the modified microecological preparation.

6. The organic fertilizer for increasing the sugar content of grape fruit according to claim 5, characterized in that: In step S1, the mass fraction of the ammonia water is 10-20%, the mass ratio of the coconut shell powder, deionized water, sodium dodecylbenzene sulfonate, zinc chloride, and ammonia water is 60-80:800-1000:4-8:10-20:50-80, the temperature of the hydrothermal carbonization reaction is 200-240°C, and the time is 4-7h; the mass concentration of nitric acid is 10-15%, the power of the ultrasonic treatment is 150-200W, and the time is 20-30min.

7. The organic fertilizer for increasing the sugar content of grape fruit according to claim 5, characterized in that: In step S2, the mass ratio of the porous biochar and vinyl triethoxysilane is 50-60:5-9, the mass fraction of ethanol in the ethanol aqueous solution is 70-80%, the temperature of the stirring reaction is 60-70°C, and the time is 1-2h; the mass ratio of the vinyl porous biochar, acrylic acid, acrylamide, and ammonium persulfate is 50-60:20-30:30-40:0.4-0.8, the temperature of the heating reaction is 70-80°C, and the time is 3-5h.

8. The organic fertilizer for increasing the sugar content of grape fruit according to claim 5, characterized in that: In step S3, the mass ratio of the β-cyclodextrin, deionized water, toluenesulfonyl chloride DMF solution, and modified porous biochar is 10-20:700-900:100-150:50-60, the mass fraction of the toluenesulfonyl chloride DMF solution is 10-20%, the temperature of the isothermal reaction is 80-90°C, and the time is 4-8h.

9. The organic fertilizer for increasing the sugar content of grape fruit according to claim 5, characterized in that: In step S4, the composite bacterial agent is mixed with Bacillus gellingii, Aspergillus oryzae and Streptomyces tenuifolius in a mass ratio of 3-6:2-4:1-3; the mass fraction of the naphthaleneacetic acid solution is 5-10%, the mass ratio of the modified carrier, the composite bacterial agent, the naphthaleneacetic acid solution and the deionized water is 50:5-10:20-30:400-500, the speed of the stirring and impregnation is 30-50r / min, the temperature is 30-40°C, the time is 1-2h, and the drying temperature is 50-60°C.

10. A method for preparing an organic fertilizer for increasing the sugar content of grape fruit as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: The raw materials are weighed according to the formula, urea, calcium magnesium phosphate fertilizer, potassium fertilizer and trace elements are mixed, stirred for 20-30 minutes, and then biomass, wood ash, decomposed livestock and poultry manure, and modified microecological preparations are added, and stirring is continued for 10-20 minutes to obtain the organic fertilizer.

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