Preparation method of antibacterial mimic peptide hydrogel

Abstract

The invention discloses a preparation method of antibacterial mimic peptide hydrogel. The preparation method comprises the following steps: S1, preparing an oxazoline mimic peptide NPX under the protection of t-butyloxycarboryl; s2.2, preparation of 2-(3-butenyl)-2-oxazoline (BOX) is carried out; s3, preparation of a t-butyloxycarboryl mimic peptide copolymer NBX; s4, removing t-butyloxycarboryl from the copolymer; and S5, preparing the mimic peptide hydrogel. Compared with the prior art, the preparation method has the advantages that the biocompatibility is improved by combining a carbonyl translocation strategy and introducing an amino bioactive group on an oxazoline skeleton, and the length of the mimic peptide is regulated and controlled by changing the quantity and proportion of monomers, so that the antibacterial and cytotoxicity of the hydrogel is macroscopically regulated and controlled. And the constructed hydrogel has good biocompatibility, and can permanently inhibit the influence of bacteria on wounds. The defects that biological peptide protein used clinically is low in hydrolytic stability, time-consuming in synthesis, difficult to prepare on a large scale and the like are overcome. In addition, the mimic peptide is used as an antibacterial agent, so that the continuous antibacterial effectiveness is ensured, the drug resistance of bacteria is reduced, and the mimic peptide is suitable for the field of biomedicine.

Description

Technical Field

[0001] The invention belongs to the field of biopolymer materials and relates to a method for preparing an antibacterial mimetic peptide hydrogel. Background Art

[0002] Wound infection is one of the main diseases that affect human health. Currently, antibiotics are mainly used for treatment. However, the hazards of long-term use of antibiotics are: 1. Bacterial resistance: After repeated exposure to antibiotics, bacteria will develop resistance to antibiotics, and eventually antibiotics will completely lose the ability to inhibit or kill bacteria. 2. Damage to human organs: While antibiotics kill bacteria, they also cause damage to the human body, such as the liver, kidneys and other organs. 3. Lead to dual infection: Under normal circumstances, there are bacterial flora in the human oral cavity, gastrointestinal tract and other parts, which restrict each other and maintain balance. If broad-spectrum antibiotics are used for a long time, it will lead to dysbiosis in the body, some sensitive bacteria will be killed, and uninhibited bacteria will take the opportunity to multiply and cause infection again. In recent years, biopeptide materials have good biocompatibility, low toxicity and multiple biological activities. They also have the functions of hormone regulation, antibacterial, antiviral, information transmission, promotion of mineral absorption, detoxification, etc., and are ideal biomaterials. Therefore, the development of biopeptide antibacterial materials has become one of the most promising ways to treat wound infections. However, clinical studies have found that there are still some problems and challenges in the actual application of biopeptides, mainly including the following aspects: 1. High production cost: At present, most biopeptide preparation methods require complex processes and equipment, and require a lot of raw materials and time; 2. Unstable biological activity: The biological activity of biopeptides is often easily affected by environmental factors and unstable, such as temperature, pH value, ionic strength, etc.; 3. Safety issues: Some biopeptides may have adverse effects on the human body, such as allergic reactions, toxicity, etc. Therefore, peptide mimetics have aroused great interest in functional research, mechanism research and variable applications.

[0003] Hydrogel is a hydrophilic three-dimensional network structure colloid that can retain a large amount of water molecules or biological fluids. Since its discovery, it has always attracted much attention. According to the source, it can be divided into natural hydrogels and artificial hydrogels. In the past 20 years, natural hydrogels have gradually been replaced by artificial hydrogels. This is because artificial hydrogels have a longer service life, high water content and high gel strength. Artificial hydrogels often have a clear molecular structure and can be further modified to obtain specific degradability and functionality. With the deepening of research on hydrogels, its characteristics are mainly reflected in the following aspects: 1. High water absorption performance: Hydrogels have extremely strong water absorption capacity and can absorb hundreds or even thousands of times their own water, quickly converting water into a gel state to form a stable gel structure. This high water absorption performance makes hydrogels have a wide range of application value in many fields. 2. Biocompatibility: Hydrogels are usually made of natural or synthetic polymer materials and have good biocompatibility. This means that hydrogels can come into contact with organisms without producing obvious toxicity or irritation reactions, so they can be used as drug sustained release, wound dressings, etc. in the medical field. 3. Adjustability: The water absorption performance of the hydrogel can be adjusted by changing the material formula, cross-linking degree, pore structure and other factors. By adjusting these factors, the hydrogel can have different water absorption speed, water retention capacity and water release characteristics to meet the needs of different application fields. If the structure of biological peptides can be simulated in the hydrogel, a more perfect antibacterial hydrogel can be designed.

[0004] Traditional hydrogels have the following problems: 1. Low mechanical properties: Natural polymer hydrogels, such as gelatin and chitosan, have good biocompatibility and degradability, but their mechanical properties are relatively low and cannot meet certain application scenarios that require higher strength materials, such as dressings; 2. Unable to meet the application requirements of dressings: When traditional hydrogels are used as dressings, due to their mechanical properties and possible biological toxicity, they may not meet the specific needs of protecting and treating wounds, especially when dealing with severely exuding wounds or infected wounds; these shortcomings limit the application of traditional hydrogels in certain fields, so the present invention adopts chemical cross-linking to improve the mechanical strength and stability of the hydrogel, and at the same time simulates the biological peptide structure in the hydrogel to improve the biocompatibility of the hydrogel and promote wound healing. Summary of the invention

[0005] Purpose of the invention: In view of the problems pointed out in the background technology, the present invention provides a method for preparing an antibacterial mimetic peptide hydrogel, combining the carbonyl translocation strategy to prepare the mimetic peptide and the butene group with the disulfide bond reaction to prepare the hydrogel, and the hydrogel and oxazoline as the substrate make the biomaterial have good biocompatibility.

[0006] Technical solution: The present invention discloses a method for preparing an antibacterial mimetic peptide hydrogel, comprising the following steps: Step S1. Preparation of tert-butyloxycarbonyl-protected oxazoline-mimetic peptide NPX: S1.1: tert-Butyloxycarbonylglycine and chloroethylamine are reactants, 1H-hydroxybenzotriazole is a polypeptide protective agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensation agent, and triethylamine is a polymerization inhibitor. The mixture is dissolved in an organic solvent A and reacted at room temperature. The product is dried and purified to obtain an intermediate product A. S1.2: reacting the intermediate product A with sodium hydroxide under a nitrogen flow, and purifying the product to obtain NPX; Step S2. Preparation of tert-butyloxycarbonyl peptide-mimicking copolymer NBX: tert-butyloxycarbonyl-protected oxazoline peptide-mimicking NPX and 2-(3-butenyl)-2-oxazoline BOX are used as reactants, methyl toluenesulfonate is used as a catalyst, dissolved in an organic solvent B, and copolymerized under a microwave synthesizer to obtain tert-butyloxycarbonyl peptide-mimicking copolymer NBX; Step S3. Preparation of a peptide-mimicking copolymer: tert-butyloxycarbonyl peptide-mimicking copolymer NBX and trifluoroacetic acid are used as reactants, the mixed solution after the reaction is shaken at room temperature, the solvent is removed under a nitrogen flow, and the residue is purified to obtain a peptide-mimicking copolymer; Step S4. Preparation of peptidomimetic hydrogel: Under aqueous conditions, dithiol threitol is used as a cross-linking agent and Irgacure 2959 solution is used as a photoinitiator to photoinitiate cross-linking of the peptidomimetic copolymer in S3 to obtain a peptidomimetic hydrogel.

[0007] Further, the preparation process of 2-(3-butenyl)-2-oxazoline BOX in step S2 is as follows: S2.1: 4-pentenoic acid and N-hydroxysuccinimide are reactants, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensing agent, dissolved in an organic solvent C, and stirred at room temperature to obtain a mixture; S2.2: Add a mixture of sodium hydroxide and ethyl chloride to the mixture obtained in S2.1, stir overnight, separate the liquids, and evaporate the solvent to obtain an intermediate product B; S2.3: The intermediate product B and potassium hydroxide are used as reactants, dissolved in an organic solvent D, and reacted under an argon flow. The obtained product is purified to obtain 2-(3-butenyl)-2-oxazoline BOX.

[0008] Furthermore, in step S1, the molar ratio of tert-butyloxycarbonylglycine and ethylchloroamine is 1:1-2; and / or the molar ratio of the intermediate product obtained in S1.1 to the sodium hydroxide input is 1:1-2; And / or the organic solvent A is any one of methanol, dichloromethane and acetonitrile.

[0009] Furthermore, in the step S2, during the preparation of 2-(3-butenyl)-2-oxazoline BOX, the molar ratio of 4-pentenoic acid to N-hydroxysuccinimide is 1:1-2, and the reaction time is more than 12 hours; And / or the organic solvent C is any one of N,N'-dimethylformamide, dichloromethane and acetonitrile.

[0010] Furthermore, in step S2, the molar ratio of the oxazoline mimetic peptide NPX and 2-(3-butenyl)-2-oxazoline BOX under the protection of tert-butyloxycarbonyl is 1 to 19:1; And / or the organic solvent B is any one of methanol, dichloromethane and acetonitrile.

[0011] Furthermore, in step S2, the microwave synthesizer is set at a temperature of 120-160° C. and a time of 10-40 min.

[0012] Furthermore, in step S3, the room temperature oscillation time is more than 2 hours.

[0013] Furthermore, in step S4, the concentration of the photoinitiator is 0.01%-0.1%, the light is 300-500nm, and the irradiation time is 400-600s.

[0014] Furthermore, in S2.2, sodium hydroxide and ethylamine are first dissolved in an appropriate amount of deionized water, and then added dropwise to the mixture of S2.1 under vigorous stirring; And / or in S2.3, the molar ratio of the intermediate product B and potassium hydroxide is 1:1, and the organic solvent D is one of methanol, dichloromethane, and acetonitrile; after the intermediate product B and potassium hydroxide are respectively dissolved in the organic solvent D, the potassium hydroxide solution is added dropwise to the solution of the intermediate product B.

[0015] Furthermore, the nitrogen flow condition is: vacuuming first and then filling with nitrogen, and repeating this process for 5 to 10 times, each time for 3 to 5 minutes, and then maintaining the nitrogen flow environment.

[0016] Beneficial Effects

[0017] The present invention relates to a method for preparing an antimicrobial mimetic peptide hydrogel, wherein a peptide mimetic is constructed using polyoxazoline through a carbonyl translocation strategy, and glycine-dependent polyoxazoline, a glycine pseudopeptide, is used as a synthetic mimetic of a biological peptide, and the good biocompatibility of glycine and polyoxazoline skeletons and the resistance of polyoxazoline to proteolysis are utilized to solve the main disadvantage of the biological peptide, namely, its poor in vivo stability.

[0018] The present invention prepares BOX to reduce production costs, uses a microwave synthesizer to improve copolymerization efficiency and yield, and through copolymerization and cross-linking of NPX and BOX, the hydrogel obtains excellent self-healing ability, good biocompatibility and biosafety, as well as excellent mechanical properties and moisturizing properties. These characteristics make it show broad application prospects in multiple fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a diagram showing the antibacterial effect of the comparative example and the experimental example of the present invention after the antibacterial test on Staphylococcus aureus; Figure 2 The antibacterial effect diagram of the comparative example and the experimental example of the present invention after being immersed in PBS solution for different time periods; DETAILED DESCRIPTION

[0020] In order to further illustrate the present invention, the following is a detailed description of a method for preparing an antibacterial mimetic peptide hydrogel provided by the present invention in combination with experimental examples. It should be understood that these experimental examples are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0021] In addition, it should be understood that after reading the contents of the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

[0022] The present invention discloses a method for preparing an antibacterial mimetic peptide hydrogel, comprising the following steps: (1) Preparation of oxazoline-mimetic peptide NPX protected by tert-butyloxycarbonyl: ① Tert-butyloxycarbonylglycine and chloroethylamine are reactants, 1H-hydroxybenzotriazole is a polypeptide protective agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensation agent, and triethylamine is an inhibitor. They are dissolved in an organic solvent A and reacted at room temperature. The product is washed and purified to obtain an intermediate product A.

[0023] ② The intermediate product A and sodium hydroxide are reacted under a nitrogen flow, and the product is purified to obtain NPX.

[0024] During the above operation, the molar ratio of tert-butyloxycarbonylglycine and ethylchloroamine is 1:1-2, the molar ratio of intermediate product A and sodium hydroxide is 1:1-2, and the organic solvent A is one of methanol, dichloromethane, and acetonitrile. The nitrogen flow condition is to first evacuate and then fill with nitrogen, and repeat this process for 5 to 10 times, each time for 3 to 5 minutes, and then maintain the nitrogen flow environment.

[0025] The reaction is carried out at room temperature, specifically: first stirring in a 0°C ice-water mixture for more than 20 minutes, then raising the temperature to room temperature, adding ethyl chloride and continuing to stir the reaction for more than 6 hours.

[0026] The product obtained by reacting the intermediate product A with sodium hydroxide under a nitrogen flow is dried in a vacuum drying oven for more than 10 hours at a temperature of 20-40°C.

[0027] (2) Preparation of 2-(3-butenyl)-2-oxazoline (BOX). Note: 2-(3-butenyl)-2-oxazoline (BOX) can be purchased from the market or prepared by the following method, specifically: ① 4-pentenoic acid and N-hydroxysuccinimide are reactants, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensing agent, dissolved in an organic solvent C, and stirred at room temperature to react to obtain a mixture. The molar ratio of 4-pentenoic acid to N-hydroxysuccinimide is 1:1-2, the reaction time is more than 12 hours, and the organic solvent C is one of N,N'-dimethylformamide, dichloromethane, and acetonitrile.

[0028] ② Add aqueous solutions of sodium hydroxide and ethylchloroamine to the obtained mixture. First, dissolve sodium hydroxide and ethylchloroamine in an appropriate amount of deionized water, and add them dropwise to the mixture under vigorous stirring. After stirring overnight, separate the liquids and evaporate the solvent to obtain intermediate product B.

[0029] ③ The intermediate product B and potassium hydroxide are used as reactants, dissolved in an organic solvent D, reacted under argon flow conditions, and the obtained product is purified to obtain BOX. The molar ratio of the intermediate product B and potassium hydroxide is 1:1, and the organic solvent D is one of methanol, dichloromethane, and acetonitrile.

[0030] When the intermediate product and potassium hydroxide are dissolved in the organic solvent D, the intermediate product B and potassium hydroxide are dissolved in the organic solvent D respectively, and then the potassium hydroxide solution is added dropwise to the solution of the intermediate product B.

[0031] The argon gas circulation condition is to first evacuate the air and then fill it with argon, and repeat this process for 5 to 10 times, each time for 3 to 5 minutes, and then maintain the argon gas circulation environment.

[0032] (3) Preparation of tert-butyloxycarbonyl peptide-mimicking copolymer (NBX): tert-butyloxycarbonyl-protected oxazoline-mimicking peptide and 2-(3-butenyl)-2-oxazoline are used as reactants, methyl toluenesulfonate is used as a catalyst, dissolved in an organic solvent B, and copolymerized under a microwave synthesizer to obtain NBX.

[0033] The molar ratio of the oxazoline mimetic peptide protected by tert-butyloxycarbonyl to 2-(3-butenyl)-2-oxazoline is 1 to 19:1, and the organic solvent B is one of methanol, dichloromethane, and acetonitrile. The temperature of the microwave synthesizer is set at 120-160°C, and the time is 10-40 minutes. The nitrogen flow condition is to first evacuate and then fill with nitrogen, and repeat this for 5 to 10 times, each time for 3 to 5 minutes, and then maintain the nitrogen flow environment.

[0034] (4). Preparation of peptide-mimicking copolymers: The copolymer and trifluoroacetic acid are reactants, and the reaction mixture is shaken at room temperature for more than 2 hours. After removing the solvent under a nitrogen flow, the residue is purified to obtain a peptide-mimicking copolymer. The nitrogen flow condition is to first evacuate and then fill with nitrogen, and repeat this process 5 to 10 times, each time for 3 to 5 minutes, and then maintain the nitrogen flow environment.

[0035] (5) Preparation of peptide mimetic hydrogel: Under aqueous conditions, dithiol threitol was used as a crosslinker and Irgacure 2959 solution was used as a photoinitiator to photoinitiate crosslinking of the peptide mimetic copolymer to obtain the peptide mimetic hydrogel. The concentration of the photoinitiator was 0.01-0.1%. The light was 300-500nm and the irradiation time was 400-600 s. Comparative Example

[0036] BOX was prepared by using 4-pentenoic acid, N-hydroxysuccinimide, chloroethylamine and potassium hydroxide as reactants, and a polymer of BOX was obtained by ring-opening polymerization using BOX as a reactant, and then cross-linked with a disulfide bond using a butenyl group to form a comparative example. That is, the comparative example only used BOX to prepare a hydrogel. Experimental Example 1

[0037] According to the above-mentioned method for preparing the antibacterial mimetic peptide hydrogel, the specific parameters are as follows: The molar ratio of tert-butyloxycarbonylglycine and ethyl chloride is 1:1.3.

[0038] The molar ratio of the intermediate product A and the sodium hydroxide input is 1:1.

[0039] Organic solvent A selection: dichloromethane.

[0040] The molar ratio of 4-pentenoic acid to N-hydroxysuccinimide is 1:1.5.

[0041] Organic solvent C selection: dichloromethane.

[0042] The molar ratio of intermediate product B and potassium hydroxide: 1:1.

[0043] Organic solvent D selection: methanol.

[0044] The tert-butyloxycarbonyl-protected oxazoline mimetic peptide NPX and 2-(3-butenyl)-2-oxazoline BOX were used as reactants, and the molar ratio was selected to be 4:1.

[0045] The organic solvent B is acetonitrile.

[0046] The concentration of photoinitiator is 0.05%.

[0047] Using tert-butyloxycarbonylglycine and ethyl chloride as reactants under the above conditions, an intermediate is prepared, and then NPX is prepared under alkaline conditions. Using 4-pentenoic acid, N-hydroxysuccinimide, ethyl chloride, and potassium hydroxide as reactants, BOX is prepared. Using NPX and BOX as reactants (molar ratio of 4:1), a simulated peptide copolymer is prepared. After removing the protecting group of the copolymer, it is cross-linked with a butenyl group and a disulfide bond to form Experimental Example 1. Experimental Example 2

[0048] The difference between Experimental Example 2 and Experimental Example 1 is that the molar ratio of NPX to BOX is 9:1, the peptide-mimicking copolymer is prepared, and after removing the protecting groups of the copolymer, the butene group is used to cross-link with disulfide bonds to form Experimental Example 2. Experimental Example 3

[0049] The difference between Experimental Example 3 and Experimental Example 1 is that the molar ratio of NPX to BOX is 19:1, the peptide-mimicking copolymer is prepared, and after removing the protecting groups of the copolymer, the butene group is used to cross-link with disulfide bonds to form Experimental Example 3.

[0050] The above four products were tested for antibacterial activity against Staphylococcus aureus. The experimental results are shown in Figure 1 ,It can be seen from the figure that the antibacterial simulated hydrogel of the present application has a good antibacterial rate.

[0051] The antibacterial effects of the above four products after being immersed in PBS solution for different time periods were tested. Figure 2 ,from Figure 2 It can be seen that the invention has good lasting antibacterial property.

[0052] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing an antibacterial mimetic peptide hydrogel, characterized in that: The following steps are involved: Step S1. Preparation of tert-butyloxycarbonyl-protected oxazoline-mimetic peptide NPX: S1.1: tert-Butyloxycarbonylglycine and chloroethylamine are reactants, 1H-hydroxybenzotriazole is a polypeptide protective agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensation agent, and triethylamine is a polymerization inhibitor. The mixture is dissolved in an organic solvent A and reacted at room temperature. The product is dried and purified to obtain an intermediate product A. S1.2: reacting the intermediate product A with sodium hydroxide under a nitrogen flow, and purifying the product to obtain NPX; Step S2. Preparation of tert-butyloxycarbonyl peptide-mimicking copolymer NBX: tert-butyloxycarbonyl-protected oxazoline peptide-mimicking NPX and 2-(3-butenyl)-2-oxazoline BOX are used as reactants, methyl toluenesulfonate is used as a catalyst, dissolved in an organic solvent B, and copolymerized under a microwave synthesizer to obtain tert-butyloxycarbonyl peptide-mimicking copolymer NBX; Step S3. Preparation of a peptide-mimicking copolymer: tert-butyloxycarbonyl peptide-mimicking copolymer NBX and trifluoroacetic acid are used as reactants, the mixed solution after the reaction is shaken at room temperature, the solvent is removed under a nitrogen flow, and the residue is purified to obtain a peptide-mimicking copolymer; Step S4. Preparation of peptidomimetic hydrogel: Under aqueous conditions, using dithiol threitol as a cross-linking agent and Irgacure 2959 solution as a photoinitiator, the peptidomimetic copolymer in S3 is subjected to photoinitiated cross-linking to obtain a peptidomimetic hydrogel.

2. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 1, characterized in that: The preparation process of 2-(3-butenyl)-2-oxazoline BOX in step S2 is as follows: S2.1: 4-pentenoic acid and N-hydroxysuccinimide are reactants, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is a condensing agent, dissolved in an organic solvent C, and stirred at room temperature to obtain a mixture; S2.2: Add a mixture of sodium hydroxide and ethyl chloride to the mixture obtained in S2.1, stir overnight, separate the liquids, and evaporate the solvent to obtain an intermediate product B; S2.3: The intermediate product B and potassium hydroxide are used as reactants, dissolved in an organic solvent D, and reacted under an argon flow. The obtained product is purified to obtain 2-(3-butenyl)-2-oxazoline BOX.

3. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 2, characterized in that: In the preparation process of the 2-(3-butenyl)-2-oxazoline BOX, the molar ratio of the 4-pentenoic acid to N-hydroxysuccinimide is 1:1-2, and the reaction time is more than 12 hours; And / or the organic solvent C is any one of N,N'-dimethylformamide, dichloromethane and acetonitrile.

4. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 2 or 3, characterized in that: In S2.2, sodium hydroxide and ethylamine are first dissolved in an appropriate amount of deionized water, and then added dropwise to the mixture in S2.1 under vigorous stirring; And / or in S2.3, the molar ratio of the intermediate product B and potassium hydroxide is 1:1, and the organic solvent D is one of methanol, dichloromethane, and acetonitrile; after the intermediate product B and potassium hydroxide are respectively dissolved in the organic solvent D, the potassium hydroxide solution is added dropwise to the solution of the intermediate product B.

5. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 1, characterized in that: In step S1, the molar ratio of tert-butyloxycarbonylglycine and ethyl chloride is 1:1-2; and / or the molar ratio of the intermediate product obtained in S1.1 to the sodium hydroxide input is 1:1-2; And / or the organic solvent A is any one of methanol, dichloromethane and acetonitrile.

6. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 1, characterized in that: In step S2, the molar ratio of the oxazoline mimetic peptide NPX and 2-(3-butenyl)-2-oxazoline BOX protected by tert-butyloxycarbonyl is 1 to 19:1; And / or the organic solvent B is any one of methanol, dichloromethane and acetonitrile.

7. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 6, characterized in that: In step S2, the microwave synthesizer is set at a temperature of 120-160° C. and a time of 10-40 min.

8. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 1, characterized in that: In step S3, the oscillation time at room temperature is more than 2 hours.

9. The method for preparing an antimicrobial mimetic peptide hydrogel according to claim 1, characterized in that: In step S4, the concentration of the photoinitiator is 0.01%-0.1%, the light is 300-500nm, and the irradiation time is 400-600s.

10. The method for preparing an antimicrobial mimetic peptide hydrogel according to any one of claims 1 to 9, characterized in that: The nitrogen flow condition is: vacuuming first and then filling with nitrogen, and repeating this process for 5 to 10 times, each time for 3 to 5 minutes, and then maintaining the nitrogen flow environment.

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