Cell stretching and compression experimental device

Abstract

The invention provides a cell stretching and compressing experimental device which structurally comprises a control mechanism, a connecting guide pipe, an electrifying shaft and an experimental mechanism, the two sides of the rear end of the control mechanism are connected with the connecting guide pipe, the electrifying shaft is arranged on the side edge of the connecting guide pipe and is in spacing fit with the connecting guide pipe, and the connecting guide pipe and the electrifying shaft are fixedly connected with the experimental mechanism through the control mechanism; the system provides axial and circumferential stress loading for various cultures of two-dimensional and three-dimensional cells and tissues; the cell culture plate has the functions of biaxial tensile force loading and uniaxial stress application, a stress loading system controlled by a PLC (programmable logic controller) provides more accurate, controllable, repeatable, static or periodic stress change for cells cultured in vitro, a linear motor is used for stretching an elastic silica gel mold at the bottom of the culture plate, the Z high extension degree of the bottom of the cell culture plate can reach 20%, and the Z high extension degree of the bottom of the cell culture plate can reach 20%. Based on the deformation of the flexible film substrate, the stress is uniform, and the reaction of cells and tissues under the stress effect can be observed in real time.

Description

Technical Field

[0001] The invention relates to the technical field of cell stretching and compression experiments, and more specifically to a cell stretching and compression experiment device. Background Art

[0002] As an important part of the current mechanics field, cell mechanics has received more and more attention in recent years with the development of society and technology. When cells are in different environments, they are affected by various mechanical factors, including tensile stress, compressive stress and other mechanical forces, which have many effects on cells. To further improve the role of mechanical factors in cell differentiation, apoptosis, and pathology, more professional instruments are needed to assist in the research. We will propose improvements to the current ordinary mechanical instruments to make them fully compatible with the research on cell stretching and compression forces, ensuring accurate data results when studying cells, and then transforming them into cell stretching and compression experimental devices. Summary of the invention

[0003] The technical solution adopted by the present invention to achieve the technical purpose is: a cell stretching and compression experimental device, whose structure includes: a control mechanism, a connecting catheter, an energized shaft, and an experimental mechanism. The two sides of the rear end of the control mechanism are connected to the connecting catheter, and the energized shaft is arranged on the side of the connecting catheter and the spacing is matched. The connecting catheter and the energized shaft are fixedly connected to the experimental mechanism through the control mechanism.

[0004] As a further improvement of the present invention, the control mechanism is provided with a positioning body, a power supply body, a human-machine control end, a connecting plate, and a connecting end. The positioning body is fixedly connected to the lower end of the power supply body, the human-machine control end and the power supply body are an integrated structure, the connecting plate carries a connecting end and is arranged at the front end of the power supply body and is on the same balance line as the human-machine control end, and the power supply body is fixedly connected with the connecting conduit and the power-carrying shaft through the connecting end of the connecting plate; the positioning body is in the shape of a "concave" character, the power supply body is in a square shape, the surface of the human-machine control end is a tempered glass product, and the shape of the connecting plate is consistent with the shape of the power supply body.

[0005] As a further improvement of the present invention, the power supply body is provided with an insulating shell, an assembly cavity, a PLC control body, a water-cooling component, a connecting body, and a guide tube. The interior of the insulating shell and the assembly cavity are an integrated structure. The PLC control body is arranged in the internal area of ​​the insulating shell through the assembly cavity. The water-cooling component is installed in the lower area of ​​the PLC control body and is matched with the spacing and is positioned and connected with the bottom layer inside the insulating shell. The connecting body is arranged in the right area of ​​the water-cooling component and is electrically connected to the human-machine control end. The guide tube is fixed to the left area of ​​the water-cooling component and is fixedly connected to the experimental mechanism. The PLC control body can complete the electrical connection with the human-machine control end and the experimental mechanism through the positioning of the insulating shell; the insulating shell is made of plastic material, and there is a safety distance between the PLC control body and the water-cooling component inside the assembly cavity. The connecting body has an energizing characteristic and contains metal wires inside.

[0006] As a further improvement of the present invention, the control body is provided with a parallel block, a power-carrying sheet, and a restraining protrusion. The center of the parallel block is penetrated by the power-carrying sheet and fixedly connected. The restraining protrusion is fixed to the surface of the power-carrying sheet and penetrates the right side of the insulating shell to complete the electrical connection with the human-machine control end. The parallel block is rectangular in shape, the length of the power-carrying sheet is smaller than the length of the parallel block and the width is larger than the width of the entire parallel block. There are three restraining protrusions on the power-carrying sheet and there is an assembly spacing between them.

[0007] As a further improvement of the present invention, the experimental mechanism is provided with an adapter end, a linear motor chassis, a mushroom-shaped telescopic head, a cell culture mold, and a mold sleeve. The adapter end and the side of the linear motor chassis are an integrated structure. The upper end of the linear motor chassis is connected to the mushroom-shaped telescopic head. The cell culture mold is arranged in the upper end area of ​​the mushroom-shaped telescopic head. The mold sleeve covers the top area of ​​the cell culture mold. The cell culture mold is positioned above the side of the control mechanism through the mushroom-shaped telescopic head and the linear motor chassis; six linear motors are contained in the linear motor chassis, the mushroom-shaped telescopic head is arranged in a vertical orientation and also has six groups. The cell culture mold is in the shape of a rectangular parallelepiped and is arranged in a parallel orientation on the top of the mushroom-shaped telescopic head. The mold sleeve is in a solid shape and its shape is consistent with the shape of the top of the cell culture mold.

[0008] As a further improvement of the present invention, the cell culture mold is provided with a penetrating wall, a parallel plate, a block, a bearing sleeve, and a cell membrane; the penetrating wall and the lower end of the parallel plate are an integrated structure; the upper end of the parallel plate and the block are stacked on each other; the bearing sleeve falls into the surface area of ​​the block and is connected with the penetrating wall; the cell membrane and the bearing sleeve are adapted to each other; the bearing sleeve is arranged above the mushroom-shaped telescopic head through the block and the parallel plate and is on the same vertical center line; the penetrating wall includes six circular through grooves; the surface of the block at the upper end of the parallel plate is finely polished and the upper end carries six groups of circular bearing sleeves.

[0009] As a further improvement of the present invention, the bearing sleeve is provided with a sleeve body, a bonding layer, a vertical block, and a limiting ring. The sleeve body and the bonding layer are an integrated structure. The vertical block is arranged on the surface edge of the sleeve body through the bonding layer. The limiting ring is fixed to the center area of ​​the sleeve body and is spaced together with the vertical block. The sleeve body is fixed to the surface area of ​​the card block through the vertical block on the bonding layer and is on the same vertical line with the mushroom-shaped telescopic head below. The bonding layer of the sleeve body is in a polished flat shape, and the surface edge of the bonding layer carries four solid vertical blocks. The diameter of the limiting ring is smaller than the diameter of the sleeve body.

[0010] Compared with the prior art, the present invention has the following beneficial effects: 1. The system provides axial and circumferential stress loading for various cultures of two-dimensional and three-dimensional cells and tissues; it not only has biaxial tensile force loading, but also has uniaxial force loading function.

[0011] 2. The PLC-controlled stress loading system provides more precise, controllable, repeatable, static or periodic stress changes for cells cultured in vitro.

[0012] 3. Use a linear motor to stretch the elastic silicone mold at the bottom of the culture plate. The maximum stretchability of the bottom of the cell culture plate can reach 20%.

[0013] 4. Based on the deformation of the flexible membrane base, the force is evenly distributed.

[0014] 5. The response of cells and tissues under stress can be observed in real time.

[0015] 6. By setting the initial position of the extrusion head, multi-channel cell compression and tensile loading functions can be combined.

[0016] 7. The instrument has up to 6 channels, and can run 6 different programs simultaneously to conduct multiple comparative experiments on different tensile deformation rates.

[0017] 8. Multiple frequencies, amplitudes and waveforms can be run in the same program.

[0018] 9. There are rich types of loading simulation waveforms: static waveform, sine waveform, cardiac waveform, triangle waveform, rectangle and various special waveforms.

[0019] 10. The PLC control system can accurately and intelligently control the loading cycle, size, frequency and duration of the tensile force.

[0020] 11. Wide range of stretch: 0-20%.

[0021] 12. Wide pulling frequency range: 0.1-2Hz (0.5Hz required).

[0022] 13. The motor temperature is controllable: 37±0.5℃. The motor is cooled by a water cooling device to avoid changes in the cell culture environment due to excessive motor temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the structure of a cell stretching and compression experimental device.

[0024] Figure 2 The present invention is a three-dimensional structural schematic diagram of an improved control mechanism.

[0025] Figure 3 The present invention is a schematic diagram of the structure of a cross-section view of the improved interior of a power supply body.

[0026] Figure 4 The present invention is a structural schematic diagram showing a front view of newly added components on both sides of a PLC control body.

[0027] Figure 5 This is a schematic diagram of the three-dimensional structure of an improved experimental mechanism.

[0028] Figure 6 The present invention is a schematic diagram of the three-dimensional structure of an improved cell culture mold.

[0029] Figure 7 The present invention is a schematic diagram of the structure of a load-bearing sleeve after improvement of the lower layer when viewed from above.

[0030] In the figure: control mechanism-1, connecting catheter-2, energized axis-3, experimental mechanism-4, positioning body-11, power supply body-12, human-machine control end-13, connecting plate-14, connecting end-15, insulating shell-121, assembly cavity-122, PLC control body-123, water cooling component-124, connecting body-125, guide tube-126, parallel block-a1, energized sheet-a2, restraining protrusion-a3, adapter end-41, linear motor chassis-42, mushroom-shaped telescopic head-43, cell culture mold-44, mold pressing sleeve-45, penetrating wall-441, parallel plate-442, block-443, bearing sleeve-444, cell membrane-445, sleeve body-b1, bonding layer-b2, vertical block-b3, limiting ring-b4. DETAILED DESCRIPTION

[0031] The present invention is further described below in conjunction with the accompanying drawings: Example

[0032] Figures 1 to 4 As shown: The present invention provides a cell stretching and compression experimental device. Its structure includes a control mechanism 1, a connecting catheter 2, an energized shaft 3, and an experimental mechanism 4. The two sides of the rear end of the control mechanism 1 are connected to the connecting catheter 2. The energized shaft 3 is arranged on the side of the connecting catheter 2 and the spacing is matched. The connecting catheter 2 and the energized shaft 3 are fixedly connected to the experimental mechanism 4 through the control mechanism 1.

[0033] The control mechanism 1 is provided with a positioning body 11, a power source body 12, a human-machine control terminal 13, a connecting plate 14, and a connecting terminal 15. The positioning body 11 is fixedly connected to the lower end of the power source body 12. The human-machine control terminal 13 and the power source body 12 are an integrated structure. The connecting plate 14 carries the connecting terminal 15 and is arranged at the front end of the power source body 12 and is on the same balance line as the human-machine control terminal 13. The power source body 12 is fixedly connected with the connecting conduit 2 and the power supply shaft 3 through the connecting terminal 15 of the connecting plate 14. The positioning body 11 is in a "concave" shape, the power source body 12 is in a square shape, the surface of the human-machine control terminal 13 is a tempered glass product, and the shape of the connecting plate 14 is consistent with that of the power source body 12. The positioning body 11 can effectively determine the position of the power supply body 12 through the "concave" shape, and the power supply body 12 can adapt to the shape of the positioning body 11 through the square shape, and then other components can be effectively assembled in order inside, so as to achieve a stable working effect. The human-machine control terminal 13 can improve the stability of the touch screen according to the surface tempered glass effect, and avoid the breakage of the screen caused by long-term touch. The connecting plate 14 can be stably installed on the side end of the power supply body 12 according to its own shape, so that the power supply body 12 can have a stable electrical control effect with the components.

[0034] The power supply body 12 is provided with an insulating shell 121, an assembly cavity 122, a PLC control body 123, a water cooling component 124, a connecting body 125, and a guide tube 126. The interior of the insulating shell 121 and the assembly cavity 122 are an integrated structure. The PLC control body 123 is arranged in the inner area of ​​the insulating shell 121 through the assembly cavity 122. The water cooling component 124 is installed in the lower area of ​​the PLC control body 123 and is spaced and matched at the same time. Positioning and connecting with the bottom layer inside the insulating shell 121, the connecting body 125 is arranged in the water cooling The right side area of ​​the assembly 124 is electrically connected to the human-machine control terminal 13, the guide tube 126 is fixed to the left side area of ​​the water-cooling assembly 124 and is fixedly connected to the experimental mechanism 4, and the PLC control body 123 can be electrically connected to the human-machine control terminal 13 and the experimental mechanism 4 through the positioning of the insulating shell 121; the insulating shell 121 is made of plastic material, and there is a safe distance between the PLC control body 123 and the water-cooling assembly 124 in the assembly cavity 122, and the connecting body 125 has an electrical characteristic, and contains metal wires inside; The insulating shell 121 is made of plastic and can effectively prevent leakage during power-on operation. The PLC control body 123 and the water cooling component 124 inside the assembly cavity 122 can prevent mutual obstruction and difficulty in later disassembly and assembly due to a safe distance. The connecting body 125 can effectively allow the components to stably control the water cooling component 124 electrically based on the internal metal wires.

[0035] The PLC control body 123 is provided with a parallel block a1, a power-carrying piece a2, and a restraining protrusion a3. The center of the parallel block a1 is penetrated by the power-carrying piece a2 and fixedly connected. The restraining protrusion a3 is fixed to the surface of the power-carrying piece a2 and penetrates the right side of the insulating shell 121 to complete the electrical connection with the human-machine control terminal 13. The parallel block a1 is rectangular in shape. The length of the power-carrying piece a2 is less than the length of the parallel block a1 and the width is greater than the width of the entire parallel block a1. There are three restraining protrusions a3 on the power-carrying piece a2 and there is an assembly spacing between them. The parallel block a1, according to its rectangular shape, allows the power-carrying piece a2 to penetrate through its own central area, so that it can form a stable "cross" assembly shape with the power-carrying piece a2, thereby improving the assembly stability and power-carrying effect with the component. The three restraining protrusions a3 on the power-carrying piece a2 can achieve a firm fit after the power-carrying piece a2 is inserted into the component, avoiding poor contact caused by shaking.

[0036] Specific functions and operation procedures of this embodiment: In the present invention, First: The cell stretching and compression experimental device installs the control mechanism 1 in a specific experimental area, and then completes the electrical connection with the experimental mechanism 4 through the connecting conduit 2 and the energized shaft 3. Therefore, the control mechanism 1 can stably control the experimental mechanism 4 through the energized shaft 3, and then use its own circulating water cooling system in combination with the connecting conduit 2 to achieve cooling of the linear motor of the experimental mechanism 4, ensuring that the experimental mechanism 4 can operate in a stable state and prevent overload caused by high temperature; Second: the control mechanism 1 can determine the position of the power source body 12 through the positioning body 11, and then the power source body 12 can use the connection end 15 of the connection plate 14 to complete the splicing with the power supply shaft 3 and the connecting conduit 2. After that, after the power source body 12 is connected to the external power supply, the human-machine control end 13 made of tempered glass can be manually touched, so that the use process of the human-machine control end 13 is as follows: 1. Select the relevant model and enter the description of the model; 2. Enter linkage settings; 3. Set manual and reset speed; 4. Enable the corresponding channel on the corresponding experimental mechanism 4; 5. Click Exit on the screen to return; 6. Set the origin of film A on the display screen so that the origin is on the film plane; 7. Click the maintenance area to enter the maintenance interface; 8. Start to find the film origin. Click the origin button of the selected channel directly. After finding the mechanical origin, the motor will locate to the film origin setting value and then set the current position to zero as the origin. If the film position has not been reached at this time, click to rise or fall directly until it reaches the position flush with the film and record the current position value. 9. Click the Exit button and add the current position value just recorded to the film origin; 10. Note the current position recorded in step 8 and add it to the film origin; 11. Click the origin button again until the action is completed, click the up button until the action stops, record the current value as the upper limit, click the origin again, return to the origin, click the down button until it stops, record the current value as the lower limit; 12. Fill the upper and lower limit values ​​recorded in step 11 into the upper and lower limit areas to play the role of limit protection; 13. Set the film working point, film stretching point (positive value), and film compression point (negative value) as required; Set the automatic speed as required; Input different machine model numbers, switch machine models, film origin, upper and lower limits, and use them directly without resetting. Different machine models can set different film working points, film compression points, and film stretching points, so that the machine model can be switched directly according to different needs without resetting parameters every time. So that through the precise debugging and cooperation of the human-machine control terminal 13, the experimental mechanism 4 can be stably controlled and the stretching and compression experiments of the cells can be performed; Third: the insulating shell 121 of the power source body 12 can avoid leakage during use based on its own insulation characteristics, and then the internal assembly cavity 122 can be used to fix the PLC control body 123 and the water cooling component 124. For this reason, the water cooling component 124 can use the connecting body 125 to allow the human-machine control end 13 to control itself, and then use the guide tube 126 to connect to the linear motor of the experimental mechanism 4. For this reason, the linear motor can be cooled when it is working to prevent high temperature. At the same time, the PLC control body 123 can use human-machine control to identify and debug the parameters and programs of the experimental mechanism 4, thereby improving the accuracy of the cell experiment; Fourth: the newly added parallel blocks a1 on both sides of the PLC control body 123 can form a stable "cross" shape with the power-carrying piece a2, and then the three restraining protrusions a3 on the power-carrying block a2 can be inserted into the human-machine control terminal 13 for electrical connection. The restraining protrusions a3 reinforce and restrain the position of the power-carrying piece a2 according to their own protruding shapes, avoiding looseness caused by the assembly gap, and ensuring that the human-machine control terminal 13 can electrically control the experimental mechanism 4 in a stable state. Example

[0037] Figures 5 to 7 As shown: The present invention provides a cell stretching and compression experimental device. Its structure includes: the experimental mechanism 4 is provided with an adapter end 41, a linear motor chassis 42, a mushroom-shaped telescopic head 43, a cell culture mold 44, and a mold sleeve 45; the adapter end 41 and the side of the linear motor chassis 42 are an integrated structure; the upper end of the linear motor chassis 42 is connected to the mushroom-shaped telescopic head 43; the cell culture mold 44 is arranged in the upper end area of ​​the mushroom-shaped telescopic head 43; the mold sleeve 45 covers the top area of ​​the cell culture mold 44; the cell culture mold 44 is positioned above the side of the control mechanism 1 through the mushroom-shaped telescopic head 43 and the linear motor chassis 42; the linear motor chassis 42 contains six linear motors, the mushroom-shaped telescopic head 43 is arranged in a vertical orientation and also has six groups; the cell culture mold 44 is a rectangular parallelepiped and is arranged in a parallel orientation on the top of the mushroom-shaped telescopic head 43; the mold sleeve 45 is solid and its shape is consistent with the shape of the top of the cell culture mold 44; The six linear motors inside the linear motor chassis 42 can effectively drive the six groups of mushroom-shaped telescopic heads 43 to move vertically, so that the mushroom-shaped telescopic heads 43 can complete the experiment of cell stretching and compression through the telescopic extrusion effect. The cell culture mold 44 can be adapted to the shape of the mold sleeve 45 at the upper end through its rectangular shape, so that the cells can be covered inside the cell culture mold 44 through the mold sleeve 45 to achieve a stable culture effect.

[0038] The cell culture mold 44 is provided with a penetrating wall 441, a parallel plate 442, a block 443, a bearing sleeve 444, and a cell membrane 445. The penetrating wall 441 and the lower end of the parallel plate 442 are an integrated structure. The upper end of the parallel plate 442 and the block 443 are stacked on each other. The bearing sleeve 444 falls into the surface area of ​​the block 443 and is connected with the penetrating wall 441. The cell membrane 445 and the bearing sleeve 444 are adapted to each other. The bearing sleeve 444 is arranged above the mushroom-shaped telescopic head 43 through the block 443 and the parallel plate 442 and is on the same vertical center line; the penetrating wall 441 includes six circular through grooves, and the surface of the block 443 at the upper end of the parallel plate 442 is finely polished and carries six groups of circular bearing sleeves 444 at the upper end; The penetrating wall 441 can allow the components to penetrate stably through six circular grooves to complete the compression and stretching of the cells. The block 443 at the upper end of the parallel plate 442 can effectively prevent the components from getting stuck due to the fine polishing of the surface. Then, six groups of cells can be loaded at the same time for experiments based on the six groups of circular carrying sleeves 444 carried, and then the comparison effect can be effectively achieved through different procedures.

[0039] The bearing sleeve 444 is provided with a sleeve body b1, a bonding layer b2, a vertical block b3, and a limiting ring b4. The sleeve body b1 and the bonding layer b2 are an integrated structure. The vertical block b3 is arranged on the surface edge of the sleeve body b1 through the bonding layer b2. The limiting ring b4 is fixed to the center area of ​​the sleeve body b1 and is spaced to match the vertical block b3. The sleeve body b1 is fixed to the surface area of ​​the clamping block 443 through the vertical block b3 on the bonding layer b2 and is on the same vertical line with the mushroom-shaped telescopic head 43 below. The bonding layer b2 of the sleeve body b1 is in a polished and flat form. The surface edge of the bonding layer b2 carries four solid vertical blocks b3. The diameter of the limiting ring b4 is smaller than the diameter of the sleeve body b1. The sleeve b1 can achieve a parallel overlap effect with the component by flattening the bonding layer b2, avoiding tilting after installation. The bonding layer b2 can be stably embedded in the component through the four solid vertical blocks b3 it carries, so as to achieve a stable fixed connection and prevent shaking and instability during tension and compression experiments on cells. The limiting ring b4 can be stably installed at the center of the sleeve b1 according to its own diameter, thereby achieving a parallel fixation effect and improving the stability of the cell position.

[0040] Specific functions and operation procedures of this embodiment: In the present invention, First: the linear motor chassis 42 of the experimental mechanism 4 can use the adapter end 41 on the side to complete the connection with the energized shaft 3 and the connecting catheter 2, and then the linear motor chassis 42 has six built-in linear motors, so that it can be adapted one by one with the six groups of mushroom-shaped telescopic heads 43 at the upper end, and then the cell culture mold 44 can be placed on the top of the mushroom-shaped telescopic head 43, and then the cartilage tissue, intervertebral disc bone tissue, tendon tissue, ligament tissue, and cells separated from muscles, lungs, heart, blood vessels, skin, tendons, ligaments, cartilage and bones can be systematically cultured in the cell culture mold 44. During the process, the mold pressure sleeve 45 on the top will cover the upper end of the cell culture mold 44 to achieve a stable culture effect, and then the human-machine control end 13 can control the linear motor inside the linear motor chassis 42, so that the linear motor performs lifting and lowering movements, driving the mushroom-shaped telescopic head 43 to move up and down, realizing the deformation of the film in the cell culture mold 44, so that it can achieve experiments on cell stretching and compression; Second: the parallel plate 442 of the cell culture mold 44 can allow the mushroom-shaped telescopic head 43 to penetrate through the lower penetrating wall 441, so that the mushroom-shaped telescopic head 43 can enter the block 443 of the parallel plate 442. For this reason, the six groups of bearing sleeves 444 on the block 443 can culture different cells. Then, the cell membrane 445 inside the bearing sleeve 444 can be combined with the penetrating wall 441 of the lower layer of the parallel plate 442 to achieve a common form with the mushroom-shaped telescopic head 43, and then the mushroom-shaped telescopic head 43 can compress and stretch the cell membrane 445 inside the bearing sleeve 444 through the penetrating wall 441, thereby stably completing the experimental process of cell stretching and compression. Finally, the parallel plate 442 can be spliced ​​with the mold pressing sleeve 45 through the top block 443, so that the mold pressing sleeve 45 can achieve an overlapping splicing effect with the cell culture mold 44, thereby improving the sealing of cell culture; Third: the lower layer of the sleeve b1 of the bearing sleeve 444 can fix the four vertical blocks b3 through the bonding layer b2, so that after the polished bonding layer b2 of the sleeve b1 is bonded with the block 443, the vertical block b3 will be vertically inserted into the interior of the block 443 to complete the vertical installation connection. For this reason, the support of the vertical block b3 can prevent the mushroom-shaped telescopic head 43 from affecting the center position of the bearing sleeve 444 when it moves up and down, ensuring that the center position of the bearing sleeve 444 will not be destroyed. Finally, the limiting ring b4 in the center area of ​​the sleeve b1 can be fixed in the center of the sleeve b1 according to its own small diameter effect, and then the position of the cell membrane 445 can be fixed by its own limiting effect, so that the center of the cell membrane 445 can be aligned with the top center of the mushroom-shaped telescopic head 43, ensuring that the center of the circle will not deviate during the experiment.

[0041] Utilizing the technical solution of the present invention, or those skilled in the art designing similar technical solutions inspired by the technical solution of the present invention to achieve the above-mentioned technical effects, all fall within the protection scope of the present invention.

Claims

1. A cell tension and compression experimental device, the structure of which includes: A control mechanism (1), a connecting conduit (2), an energized shaft (3), and an experimental mechanism (4), characterized in that: the two sides of the rear end of the control mechanism (1) are connected to the connecting conduit (2), the energized shaft (3) is arranged on the side of the connecting conduit (2) and the spacing is matched, and the connecting conduit (2) and the energized shaft (3) are fixedly connected to the experimental mechanism (4) through the control mechanism (1).

2. A cell tension and compression experimental device according to claim 1, characterized in that: The control mechanism (1) is provided with a positioning body (11), a power source body (12), a human-machine control end (13), a connecting plate (14), and a connecting end (15); the positioning body (11) is fixedly connected to the lower end of the power source body (12); the human-machine control end (13) and the power source body (12) are an integrated structure; the connecting plate (14) carries the connecting end (15) and is arranged at the front end of the power source body (12) and is on the same balance line as the human-machine control end (13); the power source body (12) is fixedly connected to the connecting conduit (2) and the energizing shaft (3) through the connecting end (15) of the connecting plate (14).

3. A cell tension and compression experimental device according to claim 2, characterized in that: The power source body (12) is provided with an insulating shell (121), an assembly cavity (122), a PLC control body (123), a water cooling component (124), a connecting body (125), and a guide tube (126); the interior of the insulating shell (121) and the assembly cavity (122) are an integrated structure; the PLC control body (123) is arranged in the inner area of ​​the insulating shell (121) through the assembly cavity (122); and the water cooling component (124) is installed in the lower area of ​​the PLC control body (123). The connecting body (125) is arranged in the right area of ​​the water cooling component (124) and is electrically connected to the human-machine control end (13). The guide tube (126) is fixed to the left area of ​​the water cooling component (124) and is fixedly connected to the experimental mechanism (4). The PLC control body (123) can be electrically connected to the human-machine control end (13) and the experimental mechanism (4) through the positioning of the insulating shell (121).

4. The cell tension and compression experimental device according to claim 3, characterized in that: The PLC control body (123) is provided with a parallel block (a1), a power-carrying sheet (a2), and a restraining protrusion (a3); the center of the parallel block (a1) is penetrated by the power-carrying sheet (a2) and fixedly connected; the restraining protrusion (a3) ​​is fixed to the surface of the power-carrying sheet (a2) and penetrates the right side of the insulating shell (121) to complete the electrical connection with the human-machine control terminal (13).

5. The cell tension and compression experimental device according to claim 1, characterized in that: The experimental mechanism (4) is provided with an adapter end (41), a linear motor chassis (42), a mushroom-shaped telescopic head (43), a cell culture mold (44), and a mold pressing sleeve (45); the adapter end (41) and the side of the linear motor chassis (42) are an integrated structure; the upper end of the linear motor chassis (42) is connected to the mushroom-shaped telescopic head (43); the cell culture mold (44) is arranged in the upper end area of ​​the mushroom-shaped telescopic head (43); the mold pressing sleeve (45) covers the top area of ​​the cell culture mold (44); and the cell culture mold (44) is positioned above the side of the control mechanism (1) through the mushroom-shaped telescopic head (43) and the linear motor chassis (42).

6. The cell tension and compression experimental device according to claim 5, characterized in that: The cell culture mold (44) is provided with a penetrating wall (441), a parallel plate (442), a block (443), a bearing sleeve (444), and a cell membrane (445); the penetrating wall (441) and the lower end of the parallel plate (442) are an integrated structure; the upper end of the parallel plate (442) and the block (443) are stacked on each other; the bearing sleeve (444) falls into the surface area of ​​the block (443) and is connected to the penetrating wall (441); the cell membrane (445) and the bearing sleeve (444) are adapted to each other; and the bearing sleeve (444) is arranged above the mushroom-shaped telescopic head (43) through the block (443) and the parallel plate (442) and is located on the same vertical center line.

7. The cell tension and compression experimental device according to claim 6, characterized in that: The bearing sleeve (444) is provided with a sleeve body (b1), a bonding layer (b2), a vertical block (b3), and a limiting ring (b4); the sleeve body (b1) and the bonding layer (b2) are an integrated structure; the vertical block (b3) is arranged on the surface edge of the sleeve body (b1) through the bonding layer (b2); the limiting ring (b4) is fixed to the center area of ​​the sleeve body (b1) and is spaced apart from the vertical block (b3); the sleeve body (b1) is fixed to the surface area of ​​the clamping block (443) through the vertical block (b3) on the bonding layer (b2) and is located on the same vertical line as the mushroom-shaped telescopic head (43) below.

Images