Method for enhancing breaking strength of silkworm silk
By injecting neodymium salt water solution in the silkworm, the breaking strength of silk is significantly improved, the problem of insufficient breaking strength of silk is solved, and the application field is broadened.
Patent Information
- Application Number
- CN202411414470.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-11
- Publication Date
- 2024-12-20
AI Technical Summary
The fracture strength of silk is low, which limits its application field. There are no reports that neodymium nitrate and neodymium chloride intake have enhanced the fracture strength of silk.
Prepare neodymium saline solution (such as neodymium nitrate or neodymium chloride aqueous solution), and use the abdominal foot injection of the silkworm to spit out silk that enhances the fracture strength. The specific steps include preparing neodymium saline solution and injecting it into the sixth abdominal section of the silkworm, waiting for cocoons to form. Reel the silk afterwards.
After injection of neodymium nitrate solution, the breaking strength of silk was enhanced from 303.07±53.54MPa to 483.39±55.07MPa, and after injection of neodymium chloride solution, the breaking strength of silk was enhanced from 410.86±83.24MPa, which significantly improved the breaking strength of silk.
Smart Images
Figure CN119162681A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of silk modification, and more specifically, relates to a method for enhancing the breaking strength of silkworm silk. Background Art
[0002] Silk is a natural fiber with good mechanical properties and biocompatibility. It is currently widely used in textiles, clothing, cosmetics, medicine and other fields. However, compared with spider silk, the breaking strength of silk is slightly inferior. Improving the breaking strength of silk will broaden its application areas. It has been reported that after silkworms ingest functional substances, they can directly spit out modified silk. For example: the mechanical strength of silk will be enhanced after ingesting materials such as graphene; after ingesting fluorescent nanoparticles, silk will fluoresce under ultraviolet light; after ingesting materials with anti-ultraviolet properties such as titanium dioxide, the anti-ultraviolet property of silk will be improved. Upon investigation, there is currently no report that the intake of neodymium nitrate and neodymium chloride has caused silkworms to directly spit out silk with enhanced breaking strength. Summary of the invention
[0003] Purpose of the invention: The present invention aims to provide a method for significantly enhancing the breaking strength of silkworm silk.
[0004] Technical solution: A method for increasing the breaking strength of silkworm silk of the present invention comprises the following steps:
[0005] (1) preparing a neodymium salt aqueous solution;
[0006] (2) The above-mentioned neodymium salt solution is injected into the ventral foot of the silkworm, and the silkworms are waited for to form cocoons and then reeled to obtain raw silk, thereby obtaining silk with enhanced breaking strength.
[0007] Furthermore, in step (1), the aqueous solution of neodymium salt is an aqueous solution of neodymium nitrate or an aqueous solution of neodymium chloride.
[0008] Furthermore, in step (1), the mass concentration of the aqueous solution of neodymium nitrate is 40 μg / μL to 90 μg / μL; the mass concentration of the aqueous solution of neodymium chloride is 40 μg / μL to 90 μg / μL.
[0009] Furthermore, in step (1), the method for preparing the aqueous solution of neodymium salt is to add water to the neodymium salt and disperse it evenly by ultrasound, so as to obtain the aqueous solution of neodymium salt.
[0010] Furthermore, in step (1), the ultrasonic time is 20-30 minutes.
[0011] Furthermore, in step (1), the water used in the aqueous solution of neodymium salt is secondary water.
[0012] Furthermore, in step (2), the location of the ventral foot injection is the ventral foot of the sixth abdominal segment of the silkworm.
[0013] Furthermore, in step (2), the dosage of the neodymium salt aqueous solution injected into each silkworm is 5 to 15 μL.
[0014] Furthermore, in step (2), the silkworm is a fifth-instar silkworm.
[0015] Furthermore, in step (2), the injection time is the 1st to 2nd day of the fifth instar of the silkworm.
[0016] Beneficial effects: Compared with the prior art, the present invention has the following significant advantages: compared with the blank control, the breaking strength of the silk after injection of neodymium nitrate solution is enhanced from 303.07±53.54MPa to 483.39±55.07MPa, and the breaking strength of the silk after injection of neodymium chloride solution is enhanced from 303.07±53.54MPa to 410.86±83.24MPa. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 1;
[0018] Figure 2 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 2;
[0019] Figure 3 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 3;
[0020] Figure 4 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 4;
[0021] Figure 5 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 5;
[0022] Figure 6 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 6;
[0023] Figure 7 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 7;
[0024] Figure 8 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 8;
[0025] Fig. 9 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 9;
[0026] Fig.10This is a schematic diagram of the stain-stress test results of the silk obtained in Example 10;
[0027] Fig.11 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 11;
[0028] Fig.12 This is a schematic diagram of the stain-stress test results of the silk obtained in Example 12;
[0029] Fig.13 This is a schematic diagram of the stain-stress test results of the silk obtained in Comparative Example 1. DETAILED DESCRIPTION
[0030] The technical solution of the present invention is further described below in conjunction with the accompanying drawings.
[0031] Example 1
[0032] This embodiment provides a method for increasing the breaking strength of silkworm silk, comprising the following steps:
[0033] (1) Preparation of materials: Weigh 0.4 g of solid neodymium nitrate and place it in a centrifuge tube. Add 10 mL of secondary water to the centrifuge tube, then ultrasonically vibrate for 20-30 minutes to prepare a neodymium nitrate aqueous solution with a concentration of 40 μg / μL.
[0034] (2) Injection of silkworms: Select silkworm larvae on the second day of the fifth instar, and inject 10 μL of neodymium nitrate aqueous solution into the silkworms using a syringe. The injection site is the ventral foot of the sixth abdominal segment of the silkworms. After the silkworms have formed cocoons, the cocoons are dried in an oven, then soaked in boiling water for 15 minutes, and then transferred to hot water at 60-70°C and soaked for about 1 hour. After the silkworms are taken out of the water, they are dried in the shade to obtain silk with enhanced breaking strength.
[0035] After obtaining the modified silk in Example 1, silk from 18 cocoons was taken and the breaking strength of the silk was tested using a tensile tester. The results are as follows: Figure 1 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 348.34±38.19 MPa.
[0036] Example 2
[0037] The specific method is basically the same as that of Example 1, except that the concentration of neodymium nitrate in step (1) is 50 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 2 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 330±42.61 MPa.
[0038] Example 3
[0039] The specific method is basically the same as that of Example 1, except that the concentration of neodymium nitrate in step (1) is 60 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 3 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 237.24±31.87 MPa.
[0040] Example 4
[0041] The specific method is basically the same as that of Example 1, except that the concentration of neodymium nitrate in step (1) is 70 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 4 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 441.13±68.40 MPa.
[0042] Example 5
[0043] The specific method is basically the same as that of Example 1, except that the concentration of neodymium nitrate in step (1) is 80 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 5 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 353.98±70.25MPa.
[0044] Example 6
[0045] The specific method is basically the same as that of Example 1, except that the concentration of neodymium nitrate in step (1) is 90 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 6 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 483.39±55.07 MPa.
[0046] Table 1
[0047]
[0048]
[0049] Example 7
[0050] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 40 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 7 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 410.86±83.24 MPa.
[0051] Example 8
[0052] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 50 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Figure 8 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 296.09±36.92 MPa.
[0053] Example 9
[0054] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 60 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Fig. 9 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 320.69±55.58 MPa.
[0055] Example 10
[0056] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 70 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Fig.10 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 298.4±53.54 MPa.
[0057] Embodiment 11
[0058] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 80 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Fig.11 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 293.9±33.22 MPa.
[0059] Example 12
[0060] The specific method is basically the same as that of Example 1, except that the solid in step (1) is neodymium chloride with a concentration of 90 μg / μL. The breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Fig.12 As shown in Table 2, the breaking strength of each group of silkworms was concentrated at 362.32±74.89 MPa.
[0061] Table 2
[0062]
[0063] Comparative Example 1
[0064] The specific implementation method is basically the same as that of Example 1, except that the solution in step (1) is a pure aqueous solution. This comparative example is a blank control group. Silk from 18 silk cocoons was taken and the breaking strength of the modified silk obtained was tested according to the method in Example 1. The results are as follows: Fig.13 As shown in Table 1, the breaking strength of each group of silkworms was concentrated at 303.07±53.54 MPa.
Claims
1. A method for increasing the breaking strength of silkworm silk, characterized in that: The steps include: (1) preparing a neodymium salt aqueous solution; (2) The above-mentioned neodymium salt solution is injected into the ventral foot of the silkworm, and the silkworms are waited for to form cocoons and then reeled to obtain raw silk, thereby obtaining silk with enhanced breaking strength.
2. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (1), the aqueous solution of neodymium salt is an aqueous solution of neodymium nitrate or an aqueous solution of neodymium chloride.
3. The method for increasing the breaking strength of silkworm silk according to claim 2, characterized in that: In step (1), the mass concentration of the aqueous solution of neodymium nitrate is 40 μg / μL to 90 μg / μL; the mass concentration of the aqueous solution of neodymium chloride is 40 μg / μL to 90 μg / μL.
4. The method for increasing the breaking strength of silkworm silk according to claim 2, characterized in that: In step (1), the method for preparing the neodymium salt aqueous solution is to add water to the neodymium salt and disperse it evenly by ultrasound, so as to obtain the neodymium salt aqueous solution.
5. The method for increasing the breaking strength of silkworm silk according to claim 4, characterized in that: In step (1), the ultrasonication time is 20-30 minutes.
6. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (1), the water used in the aqueous solution of neodymium salt is secondary water.
7. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (2), the injection site of the ventral foot is the ventral foot of the sixth abdominal segment of the silkworm.
8. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (2), the dosage of the neodymium salt aqueous solution injected into each silkworm is 5 to 15 μL.
9. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (2), the silkworm is a fifth-instar silkworm.
10. The method for increasing the breaking strength of silkworm silk according to claim 1, characterized in that: In step (2), the injection time is the 1st to 2nd day of the fifth instar of the silkworm.