A method, system and terminal for automatically adding liquid nitrogen

Abstract

The present invention relates to a liquid nitrogen automatic adding method, system and terminal, and relates to the field of automation, which includes obtaining a sensing pressure value of a preset placement area; when the sensing pressure value is greater than a preset reference pressure value, obtaining placement image information; identifying placement barcode information from a preset image recognition library according to the placement image information; matching a strain number from a preset strain database according to the placement barcode information; matching a liquid nitrogen usage value from a preset liquid nitrogen database according to the strain number; and controlling a preset transportation device to transport liquid nitrogen to a preset liquid nitrogen storage area according to the liquid nitrogen usage value. The present application has the effect of reducing operation risks.

Description

Technical Field

[0001] The present invention relates to the field of automatic control, and in particular to a liquid nitrogen automatic adding method, system and terminal. Background Art

[0002] Liquid nitrogen refers to liquid nitrogen gas, which is usually used for freezing and preserving biological samples and food, cooling industrial and scientific research experiments, material processing (such as cryogenic crushing and annealing), cryotherapy and cryogenic anesthesia in the medical field.

[0003] Currently, both strain cryopreservation and cell cryopreservation involve the use of liquid nitrogen, which will also be lost during use, requiring staff to regularly replenish the liquid nitrogen in the liquid nitrogen tank.

[0004] The liquid nitrogen tank itself is bulky and inconvenient to carry. During the adding process, the lid needs to be opened manually to pour the liquid nitrogen in. However, there are certain risks in manual operation and this needs to be improved. Summary of the invention

[0005] In order to reduce operational risks, the present invention provides a liquid nitrogen automatic adding method, system and terminal.

[0006] In a first aspect, the present invention provides a method for automatically adding liquid nitrogen, which adopts the following technical solution:

[0007] A method for automatically adding liquid nitrogen, comprising:

[0008] Obtaining the sensing pressure value of the preset placement area;

[0009] When the sensed pressure value is greater than a preset reference pressure value, obtaining placement image information;

[0010] Recognize the placement barcode information from a preset image recognition library according to the placement image information;

[0011] Match the strain number from the preset strain database according to the placement barcode information;

[0012] Match the liquid nitrogen usage value from the preset liquid nitrogen database according to the strain number;

[0013] According to the liquid nitrogen usage value, the preset transportation equipment is controlled to transport the liquid nitrogen to the preset liquid nitrogen storage area.

[0014] By adopting the above technical solution, the placement situation of the placement area can be known by understanding the relationship between the induced pressure value and the reference pressure value. When an object is placed in the placement area, the placement barcode information is obtained by obtaining the placement image information, and then the strain number is known. Then the liquid nitrogen usage value required for the strain number is known through the liquid nitrogen database, and then the transportation equipment is controlled to transport the liquid nitrogen to the liquid nitrogen storage area for use. This reduces the operational risk.

[0015] Optionally, also include:

[0016] Obtain information on the strains to be used;

[0017] According to the information of the collected strains, the strain positions are matched from the strain database, and the strain image information is obtained;

[0018] Match the current culture degree from the preset culture database according to the bacterial species image information;

[0019] Based on the situation that the current cultivation degree exceeds the preset completion cultivation degree, the preset transport device at the current strain position is controlled to transport the strain corresponding to the strain information to the preset pick-up area;

[0020] Based on the fact that the current culture level does not exceed the completed culture level, the bacterial species position is changed and the bacterial species image information is updated;

[0021] Based on the situation that the current cultivation degree matched by the updated strain image information exceeds the completed cultivation degree, the transportation device at the current strain position is controlled to transport the strain corresponding to the strain information to the taking area.

[0022] By adopting the above technical solution, when it is necessary to collect the strains, the strain location can be known by understanding the collected strain information, and the collection status of the strains can be known by understanding the consistency between the current cultivation level and the completed cultivation level. When the strains can be collected, the strains corresponding to the collected strain information are transported to the collection area by controlling the transport device so that the staff can take them, thereby improving work efficiency.

[0023] Optionally, also include:

[0024] Obtain the amount of liquid nitrogen loss;

[0025] Determine the amount of liquid nitrogen replenishment based on the amount of liquid nitrogen loss;

[0026] When the liquid nitrogen replenishment amount is not greater than a preset reference replenishment threshold, controlling the transport device to transport the liquid nitrogen to the liquid nitrogen storage area at a preset replenishment speed;

[0027] When the liquid nitrogen replenishment amount is greater than the benchmark replenishment threshold, the quotient of the liquid nitrogen replenishment amount and the benchmark replenishment threshold is calculated as the pipeline quantity value;

[0028] Based on the situation that the pipeline quantity value is not greater than the preset maximum quantity value, the preset on-off valve of the transport branch corresponding to the pipeline quantity value is opened to transport the liquid nitrogen corresponding to the liquid nitrogen replenishment amount to the liquid nitrogen storage area;

[0029] When the pipeline quantity value is greater than the maximum quantity value, the report prompts that the loss is abnormal.

[0030] By adopting the above technical solution, the transportation situation of liquid nitrogen during replenishment is known by understanding the size relationship between the liquid nitrogen replenishment amount and the reference replenishment threshold. When the liquid nitrogen replenishment amount is greater than the reference replenishment threshold, the loss of liquid nitrogen is known by understanding the size relationship between the pipeline quantity value and the maximum quantity value. And when the loss of liquid nitrogen is normal, the on-off valve of the transportation branch corresponding to the pipeline quantity value is opened to transport the liquid nitrogen corresponding to the liquid nitrogen replenishment amount to the liquid nitrogen storage area, thereby improving the efficiency of liquid nitrogen replenishment.

[0031] Optionally, also include:

[0032] After replenishing liquid nitrogen, obtain the current amount of liquid nitrogen in the liquid nitrogen tank;

[0033] When the current amount of liquid nitrogen is less than the preset amount of liquid nitrogen after replenishment, thermal image information is obtained;

[0034] Determine the leak location based on thermal image information and preset low-temperature features;

[0035] Determine the leaking object based on the leak location and the preset room layout;

[0036] Based on the fact that the leaking object is consistent with the transport equipment, the preset plugging equipment is controlled to move to the leaking location to plug the leaking location;

[0037] Based on the fact that the leaking object is consistent with the preset receiving valve, the preset output valve is controlled to close, and the preset wrapping container is controlled to move to the leaking position to wrap the receiving valve.

[0038] By adopting the above technical solution, the leakage of liquid nitrogen during replenishment can be known by understanding the relationship between the current amount of liquid nitrogen and the amount of liquid nitrogen after replenishment. When a leak occurs, the location of the leak can be known by understanding the thermal image information and low temperature characteristics, and then the leaked object can be known, so as to deal with the leaked object. This improves the safety of the experiment.

[0039] Optionally, also include:

[0040] When the current amount of liquid nitrogen is less than the preset amount of liquid nitrogen after replenishment, the difference between the amount of liquid nitrogen after replenishment and the current amount of liquid nitrogen is calculated as the amount of liquid nitrogen leakage;

[0041] Based on the situation that the leakage amount of liquid nitrogen does not exceed the preset excessive leakage threshold, the preset suction device is controlled to suction the liquid nitrogen at the leakage location according to the leakage location;

[0042] Based on the situation that the amount of liquid nitrogen leakage exceeds the excessive leakage threshold, the difference between the amount of liquid nitrogen leakage and the excessive leakage threshold is calculated as the amount of absorbed liquid nitrogen;

[0043] According to the amount of absorbed liquid nitrogen, the amount of absorption material is matched from a preset processing database;

[0044] Controlling a preset clamping device to clamp the absorbent material corresponding to the amount of absorbent material to the leakage position for absorption treatment, and obtaining a material thermal energy image of the absorbent material during absorption;

[0045] Matching the absorption material temperature from a preset absorption database according to the material thermal energy image;

[0046] Based on the fact that the temperature value of the absorption material is consistent with the preset full absorption temperature value, the suction device is controlled to suction the liquid nitrogen at the leakage position.

[0047] By adopting the above technical solution, the leakage situation can be known by understanding the excess between the liquid nitrogen leakage amount and the excessive leakage threshold. Then, different leakage situations can be handled accordingly. And when the liquid nitrogen leakage amount exceeds the excessive leakage threshold, the amount of absorption material used is known by understanding the amount of liquid nitrogen absorbed, so as to control the clamping device to clamp the absorption material corresponding to the amount of absorption material to the leakage position for absorption treatment. Then, by understanding the consistency between the temperature value of the absorption material and the full absorption temperature value, the use of the absorption material can be known. When the absorption material is fully absorbed, the suction device is controlled to suction the liquid nitrogen at the leakage position. In this way, the processing efficiency of the leaked liquid nitrogen is improved.

[0048] Optionally, also include:

[0049] After the wrapping container wraps the receiving valve, the storage amount of liquid nitrogen in the wrapping container is obtained;

[0050] Based on the preset unit time, obtain the current storage amount;

[0051] Matching the benchmark storage volume from the preset gasification database according to the unit time and the liquid nitrogen storage volume;

[0052] Based on the fact that the baseline storage volume is consistent with the current storage volume, the receiving valve is closed, and the preset clamping device is controlled to clamp the wrapped container into the preset ventilation area to handle the leaked liquid nitrogen.

[0053] By adopting the above technical solution, the reference storage volume is known by understanding the unit time and the liquid nitrogen storage volume, and the change of liquid nitrogen in the package container is known by understanding the consistency between the reference storage volume and the current storage volume. When the liquid nitrogen storage volume no longer changes, the liquid nitrogen in the package container is processed accordingly, thereby improving the safety of the experiment.

[0054] Optionally, also include:

[0055] When the sensed pressure value is greater than the reference pressure value, the placement position and placement time are obtained;

[0056] Match the culture progress from the preset culture database according to the strain number and placement time;

[0057] Matching the culture temperature from a preset temperature database according to the culture progress;

[0058] Obtaining temperature distribution information of a preset culture area;

[0059] Determine the culture position according to the culture temperature and temperature distribution information;

[0060] Matching adjustment parameters from a preset adjustment database according to the placement position and the cultivation position;

[0061] The preset adjustment device is controlled to adjust the parameters to adjust the preset incubator from the placement position to the cultivation position.

[0062] By adopting the above technical solution, the culture progress and the culture temperature can be known by knowing the strain number and placement time of the strain. Then, the culture position can be known by knowing the culture temperature and temperature distribution information, and the adjustment parameters can be known to control the adjustment device to adjust the position of the incubator, thereby improving the success rate of strain culture.

[0063] Optionally, the amount of the absorbent material needs to be calculated using a preset algorithm formula, which is:

[0064] ,in, The amount of absorbent material used, To absorb the amount of liquid nitrogen, is the correction factor, is the average liquid nitrogen absorption capacity of the absorbent material, is the coefficient of the efficiency of the absorption material in absorbing liquid nitrogen changing with time, is the coefficient of diffusion degree of leaked liquid nitrogen.

[0065] By adopting the above technical solution and using the above algorithm formula, the accuracy of the amount of absorption material is further improved, thereby saving material costs while absorbing liquid nitrogen.

[0066] In a second aspect, the present application provides a liquid nitrogen automatic addition system, which adopts the following technical solution:

[0067] A liquid nitrogen automatic adding system, comprising:

[0068] An acquisition module is used to acquire sensing pressure value, placement image information, used strain information, strain image information, liquid nitrogen loss, current liquid nitrogen amount, thermal image information, material thermal image, liquid nitrogen storage amount, current storage amount, placement position, placement time and temperature distribution information;

[0069] A memory for storing a program of any of the above-mentioned methods for automatically adding liquid nitrogen;

[0070] The program in the processor memory can be loaded and executed by the processor to implement any of the above-mentioned methods for automatically adding liquid nitrogen.

[0071] In a third aspect, the present application provides a smart terminal, which adopts the following technical solution:

[0072] An intelligent terminal comprises a memory and a processor, wherein the memory stores a computer program which can be loaded by the processor and execute any of the above-mentioned liquid nitrogen automatic adding methods.

[0073] In summary, the present application includes at least one of the following beneficial technical effects:

[0074] 1. The placement of the placement area can be known by understanding the relationship between the sensing pressure value and the reference pressure value. When an object is placed in the placement area, the placement barcode information is obtained by obtaining the placement image information, and then the strain number is known. The liquid nitrogen usage value required for the strain number is then known through the liquid nitrogen database, and the transportation equipment is controlled to transport the liquid nitrogen to the liquid nitrogen storage area for use. This reduces operational risks;

[0075] 2. The leakage situation can be known by understanding the excess between the liquid nitrogen leakage amount and the excessive leakage threshold. Then, different leakage situations can be handled accordingly. When the liquid nitrogen leakage amount exceeds the excessive leakage threshold, the amount of absorption material used can be known by understanding the amount of liquid nitrogen absorbed, so as to control the clamping device to clamp the absorption material corresponding to the amount of absorption material to the leakage position for absorption treatment. Then, the usage of the absorption material can be known by understanding the consistency between the absorption material temperature value and the full absorption temperature value. When the absorption material is full, the suction device is controlled to suction the liquid nitrogen at the leakage position. In this way, the processing efficiency of leaked liquid nitrogen is improved;

[0076] 3. By knowing the strain number and placement time of the strain, we can know the culture progress and then the culture temperature. Then, by knowing the culture temperature and temperature distribution information, we can know the culture position and then know the adjustment parameters, so as to control the adjustment equipment to adjust the position of the incubator. This improves the success rate of strain culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0077] Figure 1 is a method flow chart of a method for automatically adding liquid nitrogen in an embodiment of the present invention;

[0078] Figure 2 It is a method flow chart of the method for obtaining strains in an embodiment of the present invention;

[0079] Figure 3 is a method flow chart of a liquid nitrogen replenishment method according to an embodiment of the present invention;

[0080] Figure 4 is a method flow chart of a liquid nitrogen leak detection method in an embodiment of the present invention;

[0081] Figure 5 is a method flow chart of a method for handling liquid nitrogen leakage in an embodiment of the present invention;

[0082] Figure 6 is a method flow chart of a method for treating liquid nitrogen in a wrapped container according to an embodiment of the present invention;

[0083] Figure 7 It is a method flow chart of the strain adjustment method in an embodiment of the present invention. DETAILED DESCRIPTION

[0084] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0085] The embodiment of the present application discloses a method for automatically adding liquid nitrogen.

[0086] Reference Figure 1 , a method for automatically adding liquid nitrogen, comprising the following steps:

[0087] Step 100: Obtain the sensing pressure value of the preset placement area.

[0088] The placement area refers to the area in the liquid nitrogen tank for placing the incubator. In this embodiment, there are multiple placement areas. The incubator refers to a box for placing and protecting the bacterial culture dish. The bacterial culture dish refers to a culture vessel for placing and culturing bacterial strains. The induced pressure value refers to the pressure value on the placement area. The induced pressure value is obtained by a pressure sensor set on the placement area.

[0089] Step 101: When the sensed pressure value is greater than a preset reference pressure value, placement image information is obtained.

[0090] The reference pressure value refers to the pressure value when no object is placed in the placement area. In this embodiment, the reference pressure value is zero. The placement image information refers to the image of the incubator included in the placement area. The placement image information is obtained by taking a photo with a camera set on the liquid nitrogen tank. When the sensed pressure value is greater than the reference pressure value, it means that an incubator is placed in the placement area, and the placement image information needs to be obtained for subsequent steps.

[0091] Step 102: Identify the placement barcode information from a preset image recognition library according to the placement image information.

[0092] The placement barcode information refers to the barcode content on the incubator, and each incubator is provided with a barcode. The barcode on the incubator in the placement image information can be identified by the image recognition library, so as to identify the placement barcode information. The image recognition library is obtained based on deep learning of the neural network model. The image recognition library can identify the placement barcode image area on the incubator in the placement image information, and identify the corresponding placement barcode information from the placement barcode image area. The image recognition library is common knowledge known to those skilled in the art and will not be described in detail here.

[0093] Step 103: Match the strain number from a preset strain database according to the placement barcode information.

[0094] The strain number refers to the number corresponding to the culture dish in the incubator. The strain number corresponding to the placement barcode information can be matched through the strain database, which contains the corresponding relationship between the placement barcode information and the strain number. The strain database is a manually set database and will not be described in detail here.

[0095] Step 104: Match the liquid nitrogen usage value from a preset liquid nitrogen database according to the strain number.

[0096] The liquid nitrogen usage value refers to the amount of liquid nitrogen required for freezing and culturing the strains. The liquid nitrogen usage value corresponding to the strain number can be matched through the liquid nitrogen database, which contains the corresponding relationship between the strain number and the liquid nitrogen usage value. The liquid nitrogen database is a manually set database and will not be described here.

[0097] Step 105: Control the preset transportation equipment to transport the liquid nitrogen to the preset liquid nitrogen storage area according to the liquid nitrogen usage value.

[0098] The transport equipment refers to a transport pipeline for transporting liquid nitrogen from an external device to a liquid nitrogen tank. In this embodiment, the external device refers to a transport type liquid nitrogen tank for storing liquid nitrogen for use in the liquid nitrogen tank. There are multiple branches in the transport pipeline. In normal use, only one branch is open and the other branches are closed. The liquid nitrogen reserve area refers to an area for storing liquid nitrogen transported to the liquid nitrogen tank. The liquid nitrogen reserve area is located in the liquid nitrogen tank, and the specific location is independently set by those skilled in the art, which will not be described here. The transport equipment is controlled to transport the liquid nitrogen corresponding to the liquid nitrogen usage value to the liquid nitrogen reserve area for subsequent use.

[0099] Reference Figure 2 The method for obtaining the strains includes the following steps:

[0100] Step 200: Obtain the information of the bacterial strains to be used.

[0101] The strain information refers to the type of strain that the staff needs to take. The liquid nitrogen tank is connected to an external computer device. The staff can obtain the strain information by clicking on the computer to output the strain information to be taken to the signal transceiver terminal on the liquid nitrogen tank. The signal transceiver terminal refers to a terminal for receiving or sending signals to an external computer device. The signal transceiver terminal is set in advance by a technician in this field and will not be described in detail here.

[0102] Step 201: Match the strain location from the strain database according to the strain information, and obtain the strain image information.

[0103] The strain position refers to the position of the culture dish of the strain being used in the incubator. In the present embodiment, there are multiple strains corresponding to the strain information used, and therefore there are multiple strain positions of the same strain. The selection of the strain position in this step is obtained by matching by random selection. The strain position corresponding to the strain information used can be matched through the strain database, which includes the correspondence between the strain information used and the strain position. The strain image information refers to the real-time image of the strain at the strain position. After matching the strain position, the strain image information needs to be obtained for subsequent steps. The strain image information is obtained by taking a picture with a camera arranged in the incubator.

[0104] Step 202: Match the current culture degree from a preset culture database according to the bacterial species image information.

[0105] The current cultivation degree refers to the degree to which the strain corresponding to the strain information is currently cultivated when the strain information is obtained. The strain image information can be identified and matched to the current cultivation degree through the cultivation database. The cultivation database can identify the strain image information, and there is a corresponding relationship between the identified information and the current cultivation degree. The cultivation database is a manually set database and will not be described in detail here.

[0106] Step 203: Based on the fact that the current culture degree exceeds the preset completed culture degree, the preset transportation device at the current strain position is controlled to transport the strain corresponding to the strain information to the preset pick-up area.

[0107] The completion degree of cultivation refers to the degree value when the cultivation of the strain is completed. The transport device refers to a device used to transport the strain culture dish of the strain that has completed the cultivation to the picking area for the staff to pick up. The picking area refers to the area used to place the strain culture dish of the strain that has completed the cultivation. The completion degree of cultivation and the picking area are both set in advance by those skilled in the art and will not be described in detail here. Based on the situation that the current cultivation degree is consistent with the completion degree of cultivation, it means that the strain has been cultivated. The transport device on the strain position can be controlled to transport the strain corresponding to the strain information to the picking area for the staff to pick up.

[0108] Step 204: Based on the fact that the current culture level does not exceed the completed culture level, the position of the bacterial species is changed and the bacterial species image information is updated.

[0109] Based on the inconsistency between the current culture degree and the completed culture degree, it means that the strain corresponding to the current strain position has not been cultured. You can choose to change a strain of the same type and check it again. Therefore, you need to change the strain position and update the strain image information.

[0110] Step 205: Based on the current culture degree matched by the updated strain image information exceeding the completed culture degree, the transportation device at the current strain position is controlled to transport the strain corresponding to the strain information to the receiving area.

[0111] When the current cultivation degree matched by the updated strain image information exceeds the completed cultivation degree, it means that the strain at the strain position has been cultivated. The transport device at the strain position can be controlled to transport the strain corresponding to the strain information to the taking area for the staff to take.

[0112] Reference Figure 3 , the liquid nitrogen replenishment method comprises the following steps:

[0113] Step 300: Obtain the amount of liquid nitrogen loss.

[0114] Liquid nitrogen loss refers to the amount of liquid nitrogen consumed over time in the liquid nitrogen tank. The liquid level indicator set on the liquid nitrogen tank can be used to obtain the current amount of liquid nitrogen remaining in the liquid nitrogen tank, and the liquid nitrogen loss can be obtained by subtracting the current amount of liquid nitrogen remaining from the total amount of liquid nitrogen in the liquid nitrogen tank.

[0115] Step 301: Determine the amount of liquid nitrogen replenishment according to the amount of liquid nitrogen loss.

[0116] The amount of liquid nitrogen replenished refers to the amount of liquid nitrogen in the liquid nitrogen tank that is replenished. The amount of liquid nitrogen loss can be used to know the amount of liquid nitrogen missing in the liquid nitrogen tank, and then the amount of liquid nitrogen replenished can be obtained.

[0117] Step 302: When the liquid nitrogen replenishment amount is not greater than a preset reference replenishment threshold, control the transport equipment to transport the liquid nitrogen to the liquid nitrogen storage area at a preset replenishment speed.

[0118] The reference replenishment threshold refers to the maximum amount of liquid nitrogen that can be transported by a single transport branch of the transport equipment. The replenishment speed refers to the transport speed when replenishing liquid nitrogen. The reference replenishment threshold and the replenishment speed are both set in advance by those skilled in the art and will not be described in detail here. When the amount of liquid nitrogen replenished is not greater than the reference replenishment threshold, it means that a single branch can transport the liquid nitrogen replenishment amount of liquid nitrogen. The transport equipment is directly controlled to transport the liquid nitrogen to the liquid nitrogen storage area at the replenishment speed.

[0119] Step 303: When the liquid nitrogen replenishment amount is greater than the reference replenishment threshold, the quotient of the liquid nitrogen replenishment amount and the reference replenishment threshold is calculated as the pipeline quantity value.

[0120] The pipeline quantity value refers to the number of branches required to transport liquid nitrogen. The pipeline quantity value can be obtained by calculating the quotient of the liquid nitrogen replenishment amount divided by the benchmark replenishment threshold, and when the quotient has a decimal part, the decimal part is removed, and the integer part of the quotient is added by one as the pipeline quantity value. When the liquid nitrogen replenishment amount is greater than the benchmark replenishment threshold, it means that a single branch cannot transport the liquid nitrogen replenishment amount. The quotient of the liquid nitrogen replenishment amount and the benchmark replenishment threshold needs to be calculated as the pipeline quantity value for subsequent steps.

[0121] Step 304: Based on the situation that the pipeline quantity value is not greater than the preset maximum quantity value, the preset on-off valve of the transport branch corresponding to the pipeline quantity value is opened to transport the liquid nitrogen corresponding to the liquid nitrogen replenishment amount to the liquid nitrogen storage area.

[0122] The maximum number value refers to the number of branches in the transport equipment. The on-off valve refers to the control valve used to open or close other branches in the transport equipment. The maximum number value is set in advance by those skilled in the art and will not be described in detail here. When the pipeline number value is not greater than the maximum number value, it means that the transport equipment can carry and transport the liquid nitrogen of the liquid nitrogen replenishment amount. The on-off valve of the transport branch corresponding to the pipeline number value can be opened to transport the liquid nitrogen corresponding to the liquid nitrogen replenishment amount to the liquid nitrogen storage area.

[0123] Step 305: Based on the situation that the pipeline quantity value is greater than the maximum quantity value, report a loss abnormality.

[0124] Loss abnormality refers to the abnormality caused by excessive loss of liquid nitrogen in the liquid nitrogen tank. When the pipeline quantity value is greater than the maximum quantity value, it means that the transportation equipment cannot carry the liquid nitrogen replenishment amount. Loss abnormality needs to be reported.

[0125] Reference Figure 4 , the liquid nitrogen leak detection method includes the following steps:

[0126] Step 400: After the liquid nitrogen is replenished, the current amount of liquid nitrogen in the liquid nitrogen tank is obtained.

[0127] The current amount of liquid nitrogen refers to the amount of liquid nitrogen in the liquid nitrogen tank after it is replenished. When the liquid nitrogen tank is replenished with liquid nitrogen, the current amount of liquid nitrogen in the liquid nitrogen tank needs to be obtained for subsequent steps. The current amount of liquid nitrogen is obtained through the liquid level indicator set in the liquid nitrogen tank.

[0128] Step 401: Acquire thermal image information when the current amount of liquid nitrogen is less than a preset amount of liquid nitrogen to be replenished.

[0129] The amount of liquid nitrogen after replenishment refers to the amount of liquid nitrogen that should be in the liquid nitrogen tank after replenishment. The amount of liquid nitrogen after replenishment is set in advance by those skilled in the art and will not be elaborated here. Thermal image information refers to thermal imaging of the liquid nitrogen tank, transportation equipment, and liquid nitrogen storage container used to replenish the liquid nitrogen tank with liquid nitrogen. Thermal image information is obtained by taking pictures with a thermal camera. When the current amount of liquid nitrogen is less than the amount of liquid nitrogen after replenishment, it means that there is a leakage during the replenishment of liquid nitrogen in the liquid nitrogen tank. Thermal image information needs to be obtained for subsequent steps.

[0130] Step 402: Determine the leakage location based on the thermal image information and the preset low temperature characteristics.

[0131] The low temperature feature refers to the feature that the image on the thermal image information is affected by liquid nitrogen and causes low temperature. The low temperature feature is set in advance by a person skilled in the art and will not be described in detail here. The leakage position refers to the position where the liquid nitrogen leaks. The leakage position can be obtained by marking the low temperature feature in the thermal image information.

[0132] Step 403: Determine the leaking object according to the leaking location and the preset room layout.

[0133] The room layout refers to the distribution of various devices and equipment in the room where the liquid nitrogen tank is placed. The room layout is set in advance by a person skilled in the art and will not be described in detail here. The leaking object refers to the object that causes the liquid nitrogen leak. By comparing the leak location with the room layout, the object placed at the leak location can be known, and then the leaking object can be known.

[0134] Step 404: Based on the fact that the leaking object is consistent with the transportation equipment, control the preset blocking equipment to move to the leaking location to block the leaking location.

[0135] The plugging device is a device used to plug the location where liquid nitrogen leaks on the transport equipment. When the leaking object is consistent with the transport equipment, it means that the transport equipment is damaged, and the preset mechanical arm needs to be controlled to clamp the plugging device and move it to the leak location to plug the leak location.

[0136] Step 405: Based on the fact that the leaking object is consistent with the preset receiving valve, the preset output valve is controlled to be closed, and the preset wrapping container is controlled to move to the leaking position to wrap the receiving valve.

[0137] The receiving valve refers to the control valve used to receive liquid nitrogen from the transport equipment into the liquid nitrogen tank. The output valve refers to the control valve used to output liquid nitrogen from the external equipment to the transport equipment. The wrapping container refers to a container used to wrap the leaking control valve and absorb the leaked liquid nitrogen to prevent further leakage of liquid nitrogen. When the leaking object is consistent with the receiving valve, it means that there is a leak at the connection between the receiving valve and the transport equipment, and the output valve needs to be controlled to close to stop the delivery of liquid nitrogen. Then control the wrapping container to move to the leaking position to wrap the receiving valve to prevent further leakage of liquid nitrogen.

[0138] When the leaking object is consistent with the output valve, the subsequent processing steps are consistent with step 405.

[0139] Reference Figure 5 , the liquid nitrogen leakage treatment method includes the following steps:

[0140] Step 500: When the current amount of liquid nitrogen is less than the preset amount of liquid nitrogen after replenishment, the difference between the amount of liquid nitrogen after replenishment and the current amount of liquid nitrogen is calculated as the amount of liquid nitrogen leakage.

[0141] The amount of liquid nitrogen after replenishment refers to the amount of liquid nitrogen that should be reached in the liquid nitrogen tank after replenishment. The liquid level indicator installed on the liquid nitrogen tank can obtain the amount of liquid nitrogen in the liquid nitrogen tank before replenishment, and the amount of liquid nitrogen replenishment can be added to know the amount of liquid nitrogen after replenishment. The amount of liquid nitrogen leakage refers to the amount of liquid nitrogen leaked when liquid nitrogen leaks. The amount of liquid nitrogen leakage can be known by calculating the difference between the amount of liquid nitrogen after replenishment and the current amount of liquid nitrogen. When the current amount of liquid nitrogen is less than the amount of liquid nitrogen after replenishment, it means that liquid nitrogen leakage occurred when the liquid nitrogen tank was replenished with liquid nitrogen. The difference between the amount of liquid nitrogen after replenishment and the current amount of liquid nitrogen needs to be calculated as the amount of liquid nitrogen leakage for subsequent steps.

[0142] Step 501: Based on the fact that the leakage amount of liquid nitrogen does not exceed a preset excessive leakage threshold, a preset suction device is controlled according to the leakage location to perform suction processing on the liquid nitrogen at the leakage location.

[0143] The excessive leakage threshold refers to the volume value at which excessive leakage of liquid nitrogen is likely to affect equipment or personnel. The excessive leakage threshold is set in advance by a person skilled in the art and will not be described in detail here. The suction device refers to a device for sucking leaked liquid nitrogen. When the amount of liquid nitrogen leakage does not exceed the excessive leakage threshold, the suction device can be directly controlled to suck the leaked liquid nitrogen at the leakage location.

[0144] Step 502: Based on the situation that the liquid nitrogen leakage amount exceeds the excessive leakage threshold, the difference between the liquid nitrogen leakage amount and the excessive leakage threshold is calculated as the absorbed liquid nitrogen amount.

[0145] The amount of liquid nitrogen absorbed refers to the amount of liquid nitrogen leakage that exceeds the excessive leakage threshold, and other means are required to absorb the excess amount. The amount of liquid nitrogen absorbed can be obtained by calculating the difference between the liquid nitrogen leakage and the excessive leakage threshold. When the liquid nitrogen leakage exceeds the excessive leakage threshold, it is not easy to absorb the liquid nitrogen directly using the suction device. The difference between the liquid nitrogen leakage and the excessive leakage threshold needs to be calculated as the amount of liquid nitrogen absorbed for subsequent steps.

[0146] Step 503: Match the amount of absorption material from a preset processing database according to the amount of absorbed liquid nitrogen.

[0147] The amount of absorbent material used refers to the amount of inert material used to absorb the amount of liquid nitrogen that exceeds the excessive leakage threshold. By processing the algorithm formula between the amount of absorbed liquid nitrogen and the amount of absorbed liquid nitrogen contained in the database, the algorithm formula The amount of absorbent material used can be calculated, where The amount of absorbent material used, To absorb the amount of liquid nitrogen, In order to consider the correction factor of ambient temperature, pressure and other factors on the liquid nitrogen leakage volume, is the average liquid nitrogen absorption capacity of the absorbent material, is the coefficient of the efficiency of the absorption material in absorbing liquid nitrogen changing with time, is the coefficient of diffusion degree of leaked liquid nitrogen. , as well as The processing database is a manually set database, which will not be described here.

[0148] Step 504: Control a preset clamping device to clamp the absorbent material corresponding to the amount of absorbent material to the leakage position for absorption treatment, and obtain a material thermal energy image of the absorbent material during absorption.

[0149] The clamping device refers to a mechanical arm used to clamp the absorbent material to the leaking position so that the absorbent material absorbs the leaked liquid nitrogen. The material thermal energy image refers to the thermal energy image of the absorbent material. The material thermal energy image is obtained by taking a photo with a thermal energy camera. The clamping device is controlled to clamp the absorbent material corresponding to the amount of absorbent material to the leaking position for absorption treatment, and during the absorption, the material thermal energy image of the absorbent material is obtained for subsequent steps.

[0150] Step 505: Match the absorption material temperature from a preset absorption database according to the material thermal energy image.

[0151] The absorption material temperature refers to the temperature of the absorption material after absorbing liquid nitrogen. The absorption database can be used to identify the material thermal energy image, so that the absorption material temperature can be matched, which includes the corresponding relationship between the absorption material temperature and the material thermal energy image. The absorption database is a manually set database and will not be repeated here.

[0152] Step 506: Based on the fact that the temperature value of the absorption material is consistent with the preset full absorption temperature value, the suction device is controlled to suction the liquid nitrogen at the leakage location.

[0153] The full absorption temperature value refers to the temperature value of the absorbent material when it can no longer absorb liquid nitrogen. When the temperature value of the absorbent material is consistent with the full absorption temperature value, it means that the absorbent material can no longer absorb liquid nitrogen. The suction device can be controlled to suction the remaining liquid nitrogen at the leakage location.

[0154] Reference Figure 6 , the liquid nitrogen treatment method in the package container includes the following steps:

[0155] Step 600: After the wrapping container wraps the receiving valve, the storage amount of liquid nitrogen in the wrapping container is obtained.

[0156] The liquid nitrogen storage capacity refers to the amount of leaked liquid nitrogen stored in the wrapped container. The liquid nitrogen storage capacity is measured and obtained by the liquid level sensor set in the wrapped container. When the wrapped container is about to receive the valve wrap, the liquid nitrogen storage capacity in the wrapped container needs to be obtained for subsequent steps.

[0157] Step 601: After a preset unit time, obtain the current storage amount.

[0158] The unit time refers to the time interval for checking the liquid nitrogen storage amount in the package container. The unit time is set in advance by those skilled in the art and will not be described in detail here. After the unit time has passed, the liquid nitrogen storage amount needs to be obtained again, and the liquid nitrogen storage amount obtained again is defined as the current storage amount. In order to detect whether the liquid nitrogen in the transport equipment has completely flowed out to the package container.

[0159] Step 602: Match the reference storage volume from a preset gasification database according to the unit time and the liquid nitrogen storage volume.

[0160] The reference storage volume refers to the amount of liquid nitrogen remaining in the package container after being vaporized due to the influence of time. The vaporization database can be used to match the reference storage volume corresponding to the unit time and the liquid nitrogen storage volume, which includes the corresponding relationship between the unit time and the liquid nitrogen storage volume and the reference storage volume. The vaporization database is a manually set database and will not be described here.

[0161] Step 603: Based on the fact that the reference storage volume is consistent with the current storage volume, the receiving valve is closed, and the preset clamping device is controlled to clamp the package container into the preset ventilation area to process the leaked liquid nitrogen.

[0162] The ventilation area refers to the area used to process the collected leaked liquid nitrogen. The clamping device refers to the mechanical arm used to clamp the package container to the ventilation area. The ventilation area is set in advance by a person skilled in the art and will not be described in detail here. When the reference storage volume is consistent with the current storage volume, it means that the liquid nitrogen in the transport equipment has completely flowed out to the package container. The receiving valve needs to be closed, and the clamping device needs to be controlled to clamp the package container to the ventilation area to process the leaked liquid nitrogen.

[0163] Reference Figure 7 The strain adjustment method comprises the following steps:

[0164] Step 700: When the sensed pressure value is greater than the reference pressure value, the placement position and placement time are obtained.

[0165] The placement position refers to the position where the incubator is placed in the liquid nitrogen tank, and the placement time refers to the length of time the incubator is placed in the liquid nitrogen tank. In this embodiment, the pressure sensors on each placement area are provided with different numbers, and the placement position can be obtained by retrieving the number of the placement area where the sensed pressure value is greater than the reference pressure value. The placement time is obtained through a timer. When the sensed pressure value is greater than the reference pressure value, the timer starts timing until the placement time is needed to be retrieved, and then the placement time is output. When the sensed pressure value is greater than the reference pressure value, the placement position and placement time need to be obtained for subsequent steps.

[0166] Step 701: Match the culture progress from a preset culture database according to the strain number and placement time.

[0167] The cultivation progress refers to the development status of the strain at the current time. The cultivation database can match the strain number and the corresponding cultivation progress of the placement time, which includes the corresponding relationship between the strain number and the placement time and the cultivation progress. The cultivation database is a manually set database and will not be described here.

[0168] Step 702: Match the cultivation temperature from a preset temperature database according to the cultivation progress.

[0169] The culture temperature refers to the environmental temperature required for the strain at the current culture progress. The culture temperature corresponding to the culture progress can be matched through the temperature database, which contains the corresponding relationship between the culture progress and the culture temperature. The temperature database is a manually set database and will not be described here.

[0170] Step 703: Obtain temperature distribution information of a preset culture area.

[0171] The culture area refers to the area in the liquid nitrogen tank where the bacteria are cultured, and the culture area is the sum of all placement areas. The culture area is set in advance by a person skilled in the art and will not be described in detail here. The temperature distribution information refers to the temperature conditions at various locations in the culture area. The temperature distribution information is obtained by measuring with a temperature sensor.

[0172] Step 704: Determine the culture position according to the culture temperature and temperature distribution information.

[0173] The culture position refers to the position where the bacteria need to be adjusted due to the need to change the culture temperature. The culture temperature and temperature distribution information can be used to know the most suitable position for culturing bacteria in the culture area, and then the placement position can be updated, and the updated placement position is defined as the culture position.

[0174] Step 705: Match adjustment parameters from a preset adjustment database according to the placement position and the cultivation position.

[0175] The adjustment parameters refer to the rotation angle and displacement distance used to move the incubator containing the bacterial strains from the placement position to the cultivation position. The adjustment parameters corresponding to the placement position and the cultivation position can be matched by adjusting the database, which includes the corresponding relationship between the placement position and the cultivation position and the adjustment parameters. The adjustment database is a manually set database and will not be described in detail here.

[0176] Step 706: Control the preset adjustment device to adjust the parameters to adjust the preset incubator from the placement position to the cultivation position.

[0177] The adjusting device is a device used to move the incubator containing the bacterial strain from the placement position to the culture position, and the adjusting device is arranged in the liquid nitrogen tank. The adjusting device is controlled to adjust the parameters to adjust the incubator from the placement position to the culture position.

[0178] Based on the same inventive concept, an embodiment of the present invention provides a liquid nitrogen automatic addition system, comprising:

[0179] An acquisition module is used to acquire sensing pressure value, placement image information, used strain information, strain image information, liquid nitrogen loss, current liquid nitrogen amount, thermal image information, material thermal image, liquid nitrogen storage amount, current storage amount, placement position, placement time and temperature distribution information;

[0180] A memory for storing a program for an automatic liquid nitrogen addition method;

[0181] The program in the memory can be loaded and executed by the processor to realize a method for automatically adding liquid nitrogen.

[0182] Based on the same inventive concept, an embodiment of the present invention provides an intelligent terminal, including a memory and a processor, wherein the memory stores a computer program that can be loaded by the processor and executes a method for automatically adding liquid nitrogen.

[0183] Those skilled in the art can clearly understand that for the convenience and simplicity of description, only the division of the above-mentioned functional modules is used as an example. In actual applications, the above-mentioned functions can be assigned to different functional modules as needed, that is, the internal structure of the device is divided into different functional modules to complete all or part of the functions described above. The specific working process of the above-described system, device and unit can refer to the corresponding process in the aforementioned method embodiment, and will not be repeated here.

[0184] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A method for automatically adding liquid nitrogen, characterized in that: include: Obtaining the sensing pressure value of the preset placement area; When the sensed pressure value is greater than a preset reference pressure value, obtaining placement image information; Recognize the placement barcode information from a preset image recognition library according to the placement image information; Match the strain number from the preset strain database according to the placement barcode information; Match the liquid nitrogen usage value from the preset liquid nitrogen database according to the strain number; Controlling the preset transport equipment to transport the liquid nitrogen to the preset liquid nitrogen storage area according to the liquid nitrogen usage value; Also includes: Obtain the amount of liquid nitrogen loss; Determine the amount of liquid nitrogen replenishment based on the amount of liquid nitrogen loss; When the liquid nitrogen replenishment amount is not greater than a preset reference replenishment threshold, controlling the transport device to transport the liquid nitrogen to the liquid nitrogen storage area at a preset replenishment speed; When the liquid nitrogen replenishment amount is greater than the benchmark replenishment threshold, the quotient of the liquid nitrogen replenishment amount and the benchmark replenishment threshold is calculated as the pipeline quantity value; Based on the situation that the pipeline quantity value is not greater than the preset maximum quantity value, the preset on-off valve of the transport branch corresponding to the pipeline quantity value is opened to transport the liquid nitrogen corresponding to the liquid nitrogen replenishment amount to the liquid nitrogen storage area; If the pipeline quantity value is greater than the maximum quantity value, the report prompts abnormal loss; Also includes: After replenishing liquid nitrogen, obtain the current amount of liquid nitrogen in the liquid nitrogen tank; When the current amount of liquid nitrogen is less than the preset amount of liquid nitrogen after replenishment, thermal image information is obtained; Determine the leak location based on thermal image information and preset low-temperature features; Determine the leaking object based on the leak location and the preset room layout; Based on the fact that the leaking object is consistent with the transport equipment, the preset plugging equipment is controlled to move to the leaking location to plug the leaking location; Based on the fact that the leaking object is consistent with the preset receiving valve, the preset output valve is controlled to be closed, and the preset wrapping container is controlled to move to the leaking position to wrap the receiving valve; Also includes: When the current amount of liquid nitrogen is less than the preset amount of liquid nitrogen after replenishment, the difference between the amount of liquid nitrogen after replenishment and the current amount of liquid nitrogen is calculated as the amount of liquid nitrogen leakage; Based on the situation that the leakage amount of liquid nitrogen does not exceed the preset excessive leakage threshold, the preset suction device is controlled to suction the liquid nitrogen at the leakage location according to the leakage location; Based on the situation that the amount of liquid nitrogen leakage exceeds the excessive leakage threshold, the difference between the amount of liquid nitrogen leakage and the excessive leakage threshold is calculated as the amount of absorbed liquid nitrogen; According to the amount of absorbed liquid nitrogen, the amount of absorption material is matched from a preset processing database; Controlling a preset clamping device to clamp the absorbent material corresponding to the amount of absorbent material to the leakage position for absorption treatment, and obtaining a material thermal energy image of the absorbent material during absorption; Matching the absorption material temperature from a preset absorption database according to the material thermal energy image; Based on the fact that the temperature value of the absorption material is consistent with the preset full absorption temperature value, the suction device is controlled to suction the liquid nitrogen at the leakage position.

2. A method for automatically adding liquid nitrogen according to claim 1, characterized in that: Also includes: Obtain information on the strains to be used; According to the information of the collected strains, the strain positions are matched from the strain database, and the strain image information is obtained; Match the current culture degree from the preset culture database according to the bacterial species image information; Based on the situation that the current cultivation degree exceeds the preset completion cultivation degree, the preset transport device at the current strain position is controlled to transport the strain corresponding to the strain information to the preset pick-up area; Based on the fact that the current culture level does not exceed the completed culture level, the bacterial species position is changed and the bacterial species image information is updated; Based on the situation that the current cultivation degree matched by the updated strain image information exceeds the completed cultivation degree, the transportation device at the current strain position is controlled to transport the strain corresponding to the strain information to the taking area.

3. The method for automatically adding liquid nitrogen according to claim 1, characterized in that: Also includes: After the wrapping container wraps the receiving valve, the storage amount of liquid nitrogen in the wrapping container is obtained; Based on the preset unit time, obtain the current storage amount; Matching the benchmark storage volume from the preset gasification database according to the unit time and the liquid nitrogen storage volume; Based on the fact that the baseline storage volume is consistent with the current storage volume, the receiving valve is closed, and the preset clamping device is controlled to clamp the wrapped container into the preset ventilation area to handle the leaked liquid nitrogen.

4. The method for automatically adding liquid nitrogen according to claim 1, characterized in that: Also includes: When the sensed pressure value is greater than the reference pressure value, the placement position and placement time are obtained; Match the culture progress from the preset culture database according to the strain number and placement time; Matching the culture temperature from a preset temperature database according to the culture progress; Obtaining temperature distribution information of a preset culture area; Determine the culture position according to the culture temperature and temperature distribution information; Matching adjustment parameters from a preset adjustment database according to the placement position and the cultivation position; The preset adjustment device is controlled to adjust the parameters to adjust the preset incubator from the placement position to the cultivation position.

5. The method for automatically adding liquid nitrogen according to claim 1, characterized in that: The amount of the absorbent material needs to be calculated using a preset algorithm formula, which is: Among them, M IM is the amount of absorbent material, V LN is the amount of liquid nitrogen absorbed, C is the correction coefficient, A is the average liquid nitrogen absorption capacity of the absorbent material, K1 is the coefficient of the efficiency of the absorbent material absorbing liquid nitrogen changing with time, and K2 is the coefficient of the diffusion degree of the leaked liquid nitrogen.

6. A liquid nitrogen automatic addition system, characterized in that: include: An acquisition module is used to acquire sensing pressure value, placement image information, used strain information, strain image information, liquid nitrogen loss, current liquid nitrogen amount, thermal image information, material thermal image, liquid nitrogen storage amount, current storage amount, placement position, placement time and temperature distribution information; A memory for storing a program of a method for automatically adding liquid nitrogen according to any one of claims 1 to 5; The program in the memory can be loaded and executed by the processor to implement a method for automatically adding liquid nitrogen as described in any one of claims 1 to 5.

7. An intelligent terminal, characterized in that: The method comprises a memory and a processor, wherein the memory stores a computer program that can be loaded by the processor and executes a method for automatically adding liquid nitrogen as claimed in any one of claims 1 to 5.

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