Fluorescence detection device

Abstract

The invention discloses a fluorescence detection device which comprises a reaction flat tube, a fluorescence exciter, a fluorescence detector and a controller, and the controller is in communication connection with the fluorescence exciter and the fluorescence detector and is configured to determine the type of a fluorescent dye probe to be detected; determining the color of an excitation light beam corresponding to the fluorescent dye probe according to the type; controlling a light-emitting part corresponding to the fluorescent dye probe to emit light according to the color; determining the wave band of a fluorescent light beam corresponding to the fluorescent dye probe according to the type; and controlling a detection piece corresponding to the fluorescent dye probe to start a fluorescence detection function according to the wave band. According to the fluorescence detection device, more channel detection can be realized without adding an additional hardware structure, so that the fluorescence detection device is smaller in size, and the manufacturing cost is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular detection, and in particular to a fluorescence detection device. Background Art

[0002] With the continuous development of the field of molecular detection technology, fluorescence detection technology has become more and more perfect. The use of fluorescence detection technology (such as PCR technology) by detection personnel for fluorescence detection of nucleic acid samples has gradually exerted its unique advantages. In the process of fluorescence detection of nucleic acid samples, a fluorescence detection device is required to quantitatively analyze the target gene of the nucleic acid sample. Due to the strict requirements on the optical system during fluorescence detection, it is usually required that the wavelength of the excitation light and the wavelength of the emission light do not interfere with each other, which requires the addition of different optical filters. In some applications, it is necessary to perform multi-channel detection of the fluorescence signal in the same reaction, that is, to detect different excitation lights. This type of multi-channel detection can usually be used to detect different target genes in a sample, or to detect samples and control groups.

[0003] In order to achieve the purpose of multi-channel detection, some fluorescence detection devices contain moving parts to drive the filter to rotate, but these moving parts not only increase the cost of the fluorescence detection device, but also may bring hidden dangers to the reliability of the device during long-distance transportation and long-term use. If the filter and other parts are fixed, then in order to achieve multi-channel detection, a large number of filters need to be set, which makes the structure of the fluorescence detection device more complicated, increases the cost of the fluorescence detection device, and increases the volume of the fluorescence detection device. Summary of the invention

[0004] The object of the present invention is to provide a fluorescence detection device, which has the advantages of reducing manufacturing costs and improving reliability.

[0005] In order to achieve the above object, the present invention provides a fluorescence detection device, which includes:

[0006] A reaction flat tube, used for storing sample reagents, wherein the sample reagents include a plurality of different types of fluorescent dye probes;

[0007] The fluorescence exciter is arranged on the light-incoming side of the reaction flat tube and includes a light-emitting component and a beam-combining component. The light-emitting component includes a plurality of light-emitting components that can respectively emit excitation light beams of different colors. The beam-combining component can adjust the route of the excitation light beam and focus it so that the excitation light beam is emitted to the sample reagent. The fluorescent dye probe in the sample reagent is excited by the excitation light beam to generate a corresponding fluorescent light beam.

[0008] The fluorescence detector is arranged on the light-emitting side of the reaction flat tube and includes a detection component and a beam splitting component. The detection component includes a plurality of detection components for detecting fluorescence light beams of different wavelength bands. The beam splitting component can split the fluorescence light beam and adjust the path so that the fluorescence light beam is directed to the corresponding detection component.

[0009] The controller is connected to the fluorescence exciter and the fluorescence detector and is configured as follows:

[0010] Determining the type of fluorescent dye probe to be detected;

[0011] Determine the color of the excitation light beam corresponding to the fluorescent dye probe according to the type;

[0012] Control the light-emitting element corresponding to the fluorescent dye probe to emit light according to the color;

[0013] Determine the wavelength band of the fluorescent light beam corresponding to the fluorescent dye probe according to the type;

[0014] The detection element corresponding to the fluorescent dye probe is controlled according to the band to start the fluorescence detection function.

[0015] In an embodiment of the present invention, the plurality of light-emitting elements include a first light-emitting element, a second light-emitting element, a third light-emitting element, a fourth light-emitting element, a fifth light-emitting element, and a sixth light-emitting element. The first light-emitting element can emit a purple excitation beam, the second light-emitting element can emit a blue excitation beam, the third light-emitting element can emit a green excitation beam, the fourth light-emitting element can emit a yellow excitation beam, the fifth light-emitting element can emit a red excitation beam, and the sixth light-emitting element can emit a deep red excitation beam.

[0016] In an embodiment of the present invention, the fluorescence exciter further includes a first excitation filter, a second excitation filter, a third excitation filter, a fourth excitation filter, a fifth excitation filter and a sixth excitation filter, the first excitation filter is arranged on the light-emitting side of the first light-emitting element and is set to be a single bandpass filter with a central wavelength of 405nm and a bandwidth of 20nm, the second excitation filter is arranged on the light-emitting side of the second light-emitting element and is set to be a single bandpass filter with a central wavelength of 470nm and a bandwidth of 20nm, the third excitation filter is arranged on the light-emitting side of the third light-emitting element The fourth excitation filter is arranged on the light exit side of the fourth light emitting component and is set to be a single bandpass filter with a central wavelength of 530nm and a bandwidth of 20nm. The fourth excitation filter is arranged on the light exit side of the fourth light emitting component and is set to be a single bandpass filter with a central wavelength of 580nm and a bandwidth of 20nm. The fifth excitation filter is arranged on the light exit side of the fourth light emitting component and is set to be a single bandpass filter with a central wavelength of 630nm and a bandwidth of 20nm. The sixth excitation filter is arranged on the light exit side of the fourth light emitting component and is set to be a single bandpass filter with a central wavelength of 682nm and a bandwidth of 20nm.

[0017] In an embodiment of the present invention, the fluorescence stimulator also includes an excitation box body for accommodating a light-emitting component and a beam-combining component, and the first light-emitting component, the second light-emitting component, the third light-emitting component, the fourth light-emitting component, the fifth light-emitting component and the sixth light-emitting component are distributed in sequence along the longitudinal direction of the excitation box body and their respective light-emitting sides are all facing the beam-combining component.

[0018] In an embodiment of the present invention, a light outlet facing the light input side is formed on a side wall of the excitation box body away from the light-emitting component, and the light-emitting side of the third light-emitting component faces the light outlet; the beam combining component includes a first excitation dichroic mirror, a second excitation dichroic mirror, a third excitation dichroic mirror, a fourth excitation dichroic mirror, a fifth excitation dichroic mirror, a sixth excitation dichroic mirror, a seventh excitation dichroic mirror and a focusing cylindrical mirror, the first excitation dichroic mirror, the second excitation dichroic mirror and the third excitation dichroic mirror are arranged at intervals along the longitudinal direction of the excitation box body and are respectively located on the light paths of the first light-emitting component, the second light-emitting component and the third light-emitting component, the fourth excitation dichroic mirror, the fifth excitation dichroic mirror, the sixth excitation dichroic mirror and the seventh excitation dichroic mirror are arranged side by side along the longitudinal direction of the excitation box body and are respectively located on the light paths of the fourth light-emitting component, the fifth light-emitting component, the sixth light-emitting component and the third light-emitting component, the third excitation dichroic mirror and the seventh excitation dichroic mirror are arranged at intervals on the light path of the third light-emitting component, and the focusing cylindrical mirror is arranged between the seventh excitation dichroic mirror and the light outlet;

[0019] Among them, the first excitation dichroic mirror is used to reflect the first excitation light beam emitted by the first light-emitting component to the seventh excitation dichroic mirror; the second excitation dichroic mirror is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting component to the seventh excitation dichroic mirror; the third excitation dichroic mirror is used to transmit the third excitation light beam emitted by the third light-emitting component and reflect the first excitation light beam and the second excitation light beam to the seventh excitation dichroic mirror; the sixth excitation dichroic mirror is used to reflect the sixth excitation light beam emitted by the sixth light-emitting component to the seventh excitation dichroic mirror; the fifth excitation dichroic mirror is used to transmit the sixth excitation light beam and reflect the fifth excitation light beam emitted by the fifth light-emitting component to the seventh excitation dichroic mirror; the fourth excitation dichroic mirror is used to transmit the fifth excitation light beam and the sixth excitation light beam and reflect the fourth excitation light beam emitted by the fourth light-emitting component to the seventh excitation dichroic mirror; the seventh excitation dichroic mirror is used to transmit the first excitation light beam, the second excitation light beam, the third excitation light beam and reflect the fourth excitation light beam, the fifth excitation light beam and the sixth excitation light beam to the light outlet.

[0020] In an embodiment of the present invention, the fluorescence exciter further comprises an excitation protection glass disposed in the excitation box and located between the focusing cylindrical mirror and the light outlet.

[0021] In an embodiment of the present invention, a first receiving cavity and a second receiving cavity which are interconnected are provided in the excitation box body, the first light-emitting component, the second light-emitting component, the third light-emitting component, the fourth light-emitting component, the fifth light-emitting component and the sixth light-emitting component are all received in the first receiving cavity, the first excitation dichroic mirror, the second excitation dichroic mirror, the third excitation dichroic mirror, the fourth excitation dichroic mirror, the fifth excitation dichroic mirror, the sixth excitation dichroic mirror, the seventh excitation dichroic mirror, the focusing cylindrical mirror and the excitation protection glass are all received in the second receiving cavity, and the light outlet is connected to the second receiving cavity.

[0022] In an embodiment of the present invention, the fluorescence detector also includes a first emission filter, a second emission filter, a third emission filter, a fourth emission filter, a fifth emission filter and a sixth emission filter. The first emission filter is arranged on the signal receiving side of the first detection element of the detection component and is set to be a single bandpass filter with a central wavelength of 480nm and a bandwidth of 30nm. The second emission filter is arranged on the signal receiving side of the second detection element of the detection component and is set to be a single bandpass filter with a central wavelength of 520nm and a bandwidth of 20nm. The third emission filter is arranged on the signal receiving side of the third detection element of the detection component. The fourth emission filter is arranged on the signal receiving side of the fourth detection element of the detection component and is set to a single bandpass filter with a central wavelength of 565nm and a bandwidth of 20nm. The fifth emission filter is arranged on the signal receiving side of the fifth detection element of the detection component and is set to a single bandpass filter with a central wavelength of 615nm and a bandwidth of 40nm. The sixth emission filter is arranged on the signal receiving side of the sixth detection element of the detection component and is set to a single bandpass filter with a central wavelength of 725nm and a bandwidth of 40nm.

[0023] In an embodiment of the present invention, the fluorescence detector includes a detection box body for accommodating a detection component and a beam splitting component, and a plurality of detection components include a first detection component, a second detection component, a third detection component, a fourth detection component, a fifth detection component and a sixth detection component which are distributed in sequence along the longitudinal direction of the detection box body and whose respective signal receiving sides are facing the beam splitting component.

[0024] In an embodiment of the present invention, a light inlet facing the light emitting side and used for receiving a fluorescent light beam is provided on a side wall of one side of the detection box body, and a signal receiving side of the third detection element is directly opposite to the light inlet; the beam splitting assembly comprises a first detection dichroic mirror, a second detection dichroic mirror, a third detection dichroic mirror, a fourth detection dichroic mirror, a fifth detection dichroic mirror, a sixth detection dichroic mirror, a seventh detection dichroic mirror and an emission cylindrical mirror, the first detection dichroic mirror, the second detection dichroic mirror and the third detection dichroic mirror are arranged at intervals along the longitudinal direction of the detection box body and are respectively located on the signal receiving paths of the first detection element, the second detection element and the third detection element, the fourth detection dichroic mirror, the fifth detection dichroic mirror, the sixth detection dichroic mirror and the seventh detection dichroic mirror are arranged at intervals along the longitudinal direction of the detection box body and are respectively located on the signal receiving paths of the fourth detection element, the fifth detection element, the sixth detection element and the third detection element, the third detection dichroic mirror and the seventh detection dichroic mirror are arranged at intervals on the optical path of the third detection element, and the emission cylindrical mirror is arranged between the light inlet and the seventh detection dichroic mirror;

[0025] Among them, the seventh detection dichroic mirror is used to reflect the fourth fluorescent beam, the fifth fluorescent beam and the sixth fluorescent beam corresponding to the fourth detection component, the fifth detection component and the sixth detection component in the fluorescent beam to the fourth detection dichroic mirror, the fifth detection dichroic mirror and the sixth detection dichroic mirror, and is also used to transmit the first fluorescent beam, the second fluorescent beam and the third fluorescent beam corresponding to the first detection component, the second detection component and the third detection component in the fluorescent beam; the first detection dichroic mirror is used to reflect the first fluorescent beam to the first detection component; the second detection dichroic mirror is used to reflect the second fluorescent beam to the second detection component and transmit the first fluorescent beam; the third detection dichroic mirror is used to reflect the first fluorescent beam, the second fluorescent beam and transmit the third fluorescent beam to the third detection component; the fourth detection dichroic mirror is used to reflect the fourth fluorescent beam to the fourth detection component and transmit the fifth fluorescent beam and the sixth fluorescent beam; the fifth detection dichroic mirror is used to reflect the fifth fluorescent beam to the fifth detection component and transmit the sixth fluorescent beam; the sixth detection dichroic mirror is used to reflect the sixth fluorescent beam to the sixth detection component.

[0026] In an embodiment of the present invention, the fluorescence detector further comprises an emission protection glass which is arranged in the detection box and located between the emission cylindrical mirror and the light inlet.

[0027] In an embodiment of the present invention, the light-incoming side and the light-outgoing side are arranged at an angle of 90°.

[0028] It can be known from the above technical scheme that the fluorescence detection device includes a reaction flat tube, a fluorescence exciter, a fluorescence detector and a controller. The controller is communicatively connected with the fluorescence exciter and the fluorescence detector and is configured to: determine the type of fluorescent dye probe to be detected; determine the color of the excitation light beam corresponding to the fluorescent dye probe according to the type; control the light-emitting part corresponding to the fluorescent dye probe to emit light according to the color; determine the band of the fluorescent light beam corresponding to the fluorescent dye probe according to the type; control the detection part corresponding to the fluorescent dye probe to start the fluorescence detection function according to the band. The above fluorescence detection device can realize more channel detection without adding additional hardware structure, making the fluorescence detection device more compact, which is conducive to reducing manufacturing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings are used to provide a further understanding of the embodiments of the present invention and constitute a part of the specification. Together with the following specific implementations, they are used to explain the embodiments of the present invention, but do not constitute a limitation on the embodiments of the present invention. In the accompanying drawings:

[0030] Figure 1 is a schematic diagram of the internal structure of a fluorescence detection device in an embodiment of the present invention;

[0031] Figure 2 is a schematic diagram of the internal structure of a fluorescence exciter in an embodiment of the present invention;

[0032] Figure 3 is a schematic diagram of the overall structure of a fluorescence exciter in an embodiment of the present invention;

[0033] Figure 4 is a schematic diagram of the internal structure of a fluorescence detector in an embodiment of the present invention;

[0034] Figure 5 Schematic diagram of the overall structure of the fluorescence detector in the embodiment of the present invention.

[0035] Description of Reference Numerals

[0036] 1-reaction flat tube; 101-light inlet side; 102-light outlet side; 103-detection chamber; 2-fluorescence exciter; 201-light-emitting assembly; 2011-first light-emitting element; 2012-second light-emitting element; 2013-third light-emitting element; 2014-fourth light-emitting element; 2015-fifth light-emitting element; 2016-sixth light-emitting element; 202-beam combining assembly; 2021-first excitation dichroic mirror; 2022-second excitation dichroic mirror; 2023-third excitation dichroic mirror; 2024-fourth excitation dichroic mirror; 2025-fifth excitation dichroic mirror; 2026-sixth excitation dichroic mirror; 2027-seventh excitation dichroic mirror; 2028-focusing column mirror; 203-first excitation filter; 204-second excitation filter; 205-third excitation filter; 206-fourth excitation filter; 207-fifth excitation filter; 208-sixth excitation filter; 209-excitation box; 2091-first receiving cavity; 2092-second receiving cavity; 2093-excitation base; 2094-excitation cover; 210-light outlet; 211-excitation protection glass; 212-collimation assembly; 2121-first collimation lens; 2122-second collimation lens; 2123-third collimation lens; 2124-fourth collimation lens; 2125-fifth collimation lens; 2126-sixth collimation lens; 3-fluorescence Light detector; 301-detection assembly; 3011-first detection member; 3012-second detection member; 3013-third detection member; 3014-fourth detection member; 3015-fifth detection member; 3016-sixth detection member; 302-beam splitting assembly; 3021-first detection dichroic mirror; 3022-second detection dichroic mirror; 3023-third detection dichroic mirror; 3024-fourth detection dichroic mirror; 3025-fifth detection dichroic mirror; 3026-sixth detection dichroic mirror; 3027-seventh detection dichroic mirror; 3028-emitting cylindrical mirror; 303-detection box; 3031-third receiving cavity; 3032-fourth receiving cavity; 3033-detection base; 3034-detection cover; 304-light inlet; 305-emission protection glass; 306-first emission filter; 307-second emission filter; 308-third emission filter; 309-fourth emission filter; 310-fifth emission filter; 311-sixth emission filter; 312-emission focusing assembly; 3121-first emission focusing mirror; 3122-second emission focusing mirror; 3123-third emission focusing mirror; 3124-fourth emission focusing mirror; 3125-fifth emission focusing mirror; 3126-sixth emission focusing mirror; 4-controller; 401-first control circuit board; 402-second control circuit board. DETAILED DESCRIPTION

[0037] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0038] The present invention provides a fluorescence detection device, such as Figure 1-5 As shown, the fluorescence detection device includes:

[0039] The flat reaction tube 1 is used to store sample reagents, which include a plurality of different types of fluorescent dye probes;

[0040] The fluorescence exciter 2 is arranged on the light-incoming side 101 of the reaction flat tube 1 and includes a light-emitting component 201 and a beam-combining component 202. The light-emitting component 201 includes a plurality of light-emitting components that can respectively emit excitation light beams of different colors. The beam-combining component 202 can adjust the route of the excitation light beam and focus it so that the excitation light beam is emitted to the sample reagent. The fluorescent dye probe in the sample reagent is excited by the excitation light beam to generate a corresponding fluorescent light beam.

[0041] The fluorescence detector 3 is arranged on the light-emitting side 102 of the reaction flat tube 1 and includes a detection component 301 and a beam splitting component 302. The detection component 301 includes a plurality of detection components for detecting fluorescence light beams of different wavelength bands. The beam splitting component 302 can split the fluorescence light beam and adjust the path so that the fluorescence light beam is directed to the corresponding detection component.

[0042] The controller 4 is connected to the fluorescence exciter 2 and the fluorescence detector 3 and is configured as follows:

[0043] Determining the type of fluorescent dye probe to be detected;

[0044] Determine the color of the excitation light beam corresponding to the fluorescent dye probe according to the type;

[0045] Control the light-emitting element corresponding to the fluorescent dye probe to emit light according to the color;

[0046] Determine the wavelength band of the fluorescent light beam corresponding to the fluorescent dye probe according to the type;

[0047] The detection element corresponding to the fluorescent dye probe is controlled according to the band to start the fluorescence detection function.

[0049] Specifically, a detection cavity 103 for storing sample reagents is formed on the reaction flat tube 1 in the present embodiment; the number of light-emitting parts is preferably six, and the fluorescence stimulator 2 is provided with excitation channels corresponding to each light-emitting part, and the number of excitation channels is consistent with the number of light-emitting parts; correspondingly, the number of detection parts is also six, and the detection stimulator is provided with emission channels corresponding to each detection part, and the number of emission channels is consistent with the number of detection parts; the types of fluorescent dye probes in the sample reagent can be greater than six, and the controller 4 pre-stores the colors of the excitation light beams and the wavelength bands of the fluorescent light beams corresponding to various fluorescent dye probes, respectively. One fluorescent dye probe corresponds to one detection channel during detection, and the detection channel consists of an excitation channel and an emission channel.

[0050] When performing the test, the tester first places the sample reagent in the detection chamber 103 of the reaction flat tube 1, and then inputs the type of the fluorescent dye probe to be detected into the controller 4. The controller 4 determines the type of the fluorescent dye probe to be detected, and determines the color of the excitation light beam and the wavelength band of the fluorescent light beam corresponding to the fluorescent dye probe to be detected according to the type. Then, the controller 4 sends a control instruction to the corresponding light-emitting component and the detection component, so that the corresponding light-emitting component emits light and the corresponding detection component starts the detection function.

[0051] Furthermore, after the light-emitting component emits an excitation light beam, the beam combining component 202 adjusts the path and focuses the light beam, so that the excitation light beam passes through a first preset path (i.e., the excitation channel) and is focused and emitted to the light-incoming side 101 of the reaction flat tube 1, and enters the detection cavity 103 from the light-incoming side 101 to excite the fluorescent dye probe to be detected contained in the sample reagent in the detection cavity 103. After being excited, the fluorescent dye probe to be detected emits a corresponding fluorescent light beam, which is emitted from the light-emitting side 102 of the reaction flat tube 1 and enters the fluorescence detector 3; the fluorescent light beam entering the fluorescence detector 3 is adjusted in path by the beam splitting component 302, and then passes through the second preset path (i.e., the emission channel) and then emitted to the detection component.

[0052] Since the emission channel opened by the fluorescence detection device in this embodiment during detection is not determined by the excitation channel but by the type of fluorescent dye probe to be detected, one excitation channel can correspond to multiple different emission channels. For example, when there are many types of fluorescent dye probes in the sample reagent (such as seven, eight or other larger numbers) and at least two of them correspond to the same light-emitting element in the fluorescence exciter 2 (such as fluorescent dye probes corresponding to FRET dye or AttoPhos dye), but the above two fluorescent dye probes correspond to two different detection elements in the fluorescence detector 3, then one excitation channel can be expanded into two detection channels. In this way, the number of detection channels of the fluorescence detection device in this embodiment is greatly expanded, and is not limited to six (at this time, one excitation channel can only correspond to one emission channel), that is, more channel detection can be achieved without adding additional hardware structure. In other words, more detection channels can be expanded while ensuring that the fluorescence detection device is compact and has low manufacturing cost.

[0053] In one embodiment of the present invention, the plurality of light-emitting elements include a first light-emitting element 2011, a second light-emitting element 2012, a third light-emitting element 2013, a fourth light-emitting element 2014, a fifth light-emitting element 2015, and a sixth light-emitting element 2016. The first light-emitting element 2011 can emit a purple excitation light beam, which can excite the fluorescent dye probe corresponding to ATTO 425 dye or Super Bright 436 dye; the second light-emitting element 2012 can emit a blue excitation light beam, which can excite the fluorescent dye probe corresponding to FAM dye; the third light-emitting element 2013 can emit a green excitation light beam, which can excite the fluorescent dye probe corresponding to HEX dye, VIC dye, FRET dye or AttoPhos dye; the fourth light-emitting element 2014 can emit a yellow excitation light beam, which can excite the fluorescent dye probe corresponding to ROX dye; the fifth light-emitting element 2015 can emit a red excitation light beam, which can excite the fluorescent dye probe corresponding to CY5 dye or SYPRO The sixth light-emitting element 2016 can emit a deep red excitation beam, which can excite the fluorescent dye probe corresponding to the Quasar705 or CY5.5 dye. Among them, the first light-emitting element 2011 can be a purple LED lamp, the second light-emitting element 2012 can be a blue LED lamp, the third light-emitting element 2013 can be a green LED lamp, the fourth light-emitting element 2014 can be a yellow LED lamp, the fifth light-emitting element 2015 can be a red LED lamp, and the sixth light-emitting element 2016 can be a deep red LED lamp; further, each light-emitting element in this embodiment can also be selected as a laser lamp, a halogen lamp or other types of light sources.

[0054] In one embodiment of the present invention, the fluorescence exciter 2 also includes a first excitation filter 203, a second excitation filter 204, a third excitation filter 205, a fourth excitation filter 206, a fifth excitation filter 207 and a sixth excitation filter 208, the first excitation filter 203 is arranged on the light-emitting side of the first light-emitting element 2011 and is set to a single bandpass filter with a central wavelength of 405nm and a bandwidth of 20nm, the second excitation filter 204 is arranged on the light-emitting side of the second light-emitting element 2012 and is set to a single bandpass filter with a central wavelength of 470nm and a bandwidth of 20nm, the third excitation filter 205 is arranged on the light-emitting side of the third light-emitting element 2013 and is set to a single bandpass filter with a central wavelength of 530nm and a bandwidth of 20nm. The fourth excitation filter 206 is arranged on the light-emitting side of the fourth light-emitting element 2014 and is set to a single bandpass filter with a central wavelength of 580nm and a bandwidth of 20nm. The fifth excitation filter 207 is arranged on the light-emitting side of the fourth light-emitting element 2014 and is set to a single bandpass filter with a central wavelength of 630nm and a bandwidth of 20nm. The sixth excitation filter 208 is arranged on the light-emitting side of the fourth light-emitting element 2014 and is set to a single bandpass filter with a central wavelength of 682nm and a bandwidth of 20nm. Through the filtering effect of each filter, the excitation light beam emitted by each light-emitting element can accurately meet the specific wavelength requirements, which can effectively improve the accuracy and sensitivity of the excitation of the fluorescence exciter 2, thereby improving the detection accuracy and sensitivity of the fluorescence detection device.

[0055] In one embodiment of the present invention, Figure 3 As shown, the fluorescence stimulator 2 also includes an excitation box body 209 for accommodating the light-emitting component 201 and the beam-combining component 202, and the controller 4 includes a first control circuit board 401 that is communicatively connected to each light-emitting component. The first light-emitting component 2011, the second light-emitting component 2012, the third light-emitting component 2013, the fourth light-emitting component 2014, the fifth light-emitting component 2015 and the sixth light-emitting component 2016 are sequentially distributed along the longitudinal direction of the excitation box body 209 and their respective light-emitting sides are all facing the beam-combining component 202. This structural arrangement can facilitate wiring between each light-emitting component and the first control circuit board 401, simplify the circuit layout of the fluorescence stimulator 2, and facilitate the automated production line production and installation of the fluorescence stimulator 2.

[0056] In one embodiment of the present invention, the excitation box body 209 is formed with a light outlet 210 facing the light incident side 101 on a side wall away from the light-emitting component 201, and the light-emitting side of the third light-emitting component 2013 is opposite to the light outlet 210; the beam-combining component 202 includes a first excitation dichroic mirror 2021, a second excitation dichroic mirror 2022, a third excitation dichroic mirror 2023, a fourth excitation dichroic mirror 2024, a fifth excitation dichroic mirror 2025, a sixth excitation dichroic mirror 2026, a seventh excitation dichroic mirror 2027 and a focusing cylindrical mirror 2028, and the first excitation dichroic mirror 2021, the second excitation dichroic mirror 2022 and the third excitation dichroic mirror 2023 are arranged at intervals along the longitudinal direction of the excitation box body 209 and correspond to the first light-emitting component 2011, the second light-emitting component 2013 and the light-emitting side thereof. The fourth excitation dichroic mirror 2024, the fifth excitation dichroic mirror 2025, the sixth excitation dichroic mirror 2026 and the seventh excitation dichroic mirror 2027 are arranged side by side along the longitudinal direction of the excitation box body 209 and are respectively located on the light paths of the fourth light-emitting member 2014, the fifth light-emitting member 2015, the sixth light-emitting member 2016 and the third light-emitting member 2013. The third excitation dichroic mirror 2023 and the seventh excitation dichroic mirror 2027 are arranged at intervals on the light path of the third light-emitting member 2013. The focusing cylindrical mirror 2028 is arranged between the seventh excitation dichroic mirror 2027 and the light outlet 210, and is used to focus the excitation light beam emitted along the light outlet 210 into a linear light source, so as to improve the accuracy of the excitation light beam projected to the sample reagent in the reaction flat tube 1;

[0057] The first excitation dichroic mirror 2021 is used to reflect the first excitation light beam emitted by the first light-emitting element 2011 to the seventh excitation dichroic mirror 2027; the second excitation dichroic mirror 2022 is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting element 2012 to the seventh excitation dichroic mirror 2027; the third excitation dichroic mirror 2023 is used to transmit the third excitation light beam emitted by the third light-emitting element 2013 and reflect the first excitation light beam and the second excitation light beam to the seventh excitation dichroic mirror 2027; the sixth excitation dichroic mirror 2026 is used to transmit the sixth excitation light beam emitted by the sixth light-emitting element 2016 to the seventh excitation dichroic mirror 2027. The beam is reflected toward the seventh excitation dichroic mirror 2027; the fifth excitation dichroic mirror 2025 is used to transmit the sixth excitation beam and reflect the fifth excitation beam emitted by the fifth light-emitting element 2015 toward the seventh excitation dichroic mirror 2027; the fourth excitation dichroic mirror 2024 is used to transmit the fifth excitation beam and the sixth excitation beam and reflect the fourth excitation beam emitted by the fourth light-emitting element 2014 toward the seventh excitation dichroic mirror 2027; the seventh excitation dichroic mirror 2027 is used to transmit the first excitation beam, the second excitation beam, and the third excitation beam and reflect the fourth excitation beam, the fifth excitation beam, and the sixth excitation beam toward the light outlet 210. Through the reflection and / or transmission effect of the above-mentioned first excitation dichroic mirror 2021, the second excitation dichroic mirror 2022, the third excitation dichroic mirror 2023, the fourth excitation dichroic mirror 2024, the fifth excitation dichroic mirror 2025, the sixth excitation dichroic mirror 2026 and the seventh excitation dichroic mirror 2027, the paths of the excitation light beams emitted by the first light-emitting element 2011, the second light-emitting element 2012, the third light-emitting element 2013, the fourth light-emitting element 2014, the fifth light-emitting element 2015 and the sixth light-emitting element 2016 are adjusted so that they can be emitted along the light outlet 210.

[0058] In one embodiment of the present invention, the fluorescence exciter 2 also includes a collimating component 212 disposed in the excitation box body 209, and the collimating component 212 includes a first collimating lens 2121, a second collimating lens 2122, a third collimating lens 2123, a fourth collimating lens 2124, a fifth collimating lens 2125 and a sixth collimating lens 2126, wherein the first collimating lens 2121 is disposed between the first light-emitting element 2011 and the first excitation filter 203, and is used to collimate the excitation light beam emitted by the first light-emitting element 2011 into parallel light; the second collimating lens 2122 is disposed between the second light-emitting element 2012 and the second excitation filter 204, and is used to collimate the excitation light beam emitted by the second light-emitting element 2012 into parallel light; and the third collimating lens 2124 is disposed between the second light-emitting element 2012 and the second excitation filter 204, and is used to collimate the excitation light beam emitted by the second light-emitting element 2012 into parallel light. The collimating lens 2123 is arranged between the third light-emitting element 2013 and the third excitation filter 205, and is used for collimating the excitation light beam emitted by the third light-emitting element 2013 into parallel light; the fourth collimating lens 2124 is arranged between the fourth light-emitting element 2014 and the fourth excitation filter 206, and is used for collimating the excitation light beam emitted by the fourth light-emitting element 2014 into parallel light; the fifth collimating lens 2125 is arranged between the fifth light-emitting element 2015 and the fifth excitation filter 207, and is used for collimating the excitation light beam emitted by the fifth light-emitting element 2015 into parallel light; the sixth collimating lens 2126 is arranged between the sixth light-emitting element 2016 and the sixth excitation filter 208, and is used for collimating the excitation light beam emitted by the sixth light-emitting element 2016 into parallel light.

[0059] In one embodiment of the present invention, the fluorescence exciter 2 also includes an excitation protection glass 211 arranged in the excitation box body 209 and located between the focusing cylindrical mirror 2028 and the light outlet 210. The excitation protection glass 211 can extend the focal length and prevent the heat generated by the sample reagent in the reaction flat tube 1 when being heated from affecting the focusing effect of the focusing cylindrical mirror 2028.

[0060] In one embodiment of the present invention, a first receiving cavity 2091 and a second receiving cavity 2092 are provided in the excitation box body 209, which are interconnected. The first light-emitting component 2011, the second light-emitting component 2012, the third light-emitting component 2013, the fourth light-emitting component 2014, the fifth light-emitting component 2015 and the sixth light-emitting component 2016 are all received in the first receiving cavity 2091, the first excitation dichroic mirror 2021, the second excitation dichroic mirror 2022, the third excitation dichroic mirror 2023, the fourth excitation dichroic mirror 2024, the fifth excitation dichroic mirror 2025, the sixth excitation dichroic mirror 2026, the seventh excitation dichroic mirror 2027, the focusing cylindrical mirror 2028 and the excitation protection glass 211 are all received in the second receiving cavity 2092, and the light outlet 210 is connected to the second receiving cavity 2092. Specifically, the excitation box body 209 includes a detachably connected excitation base 2093 and an excitation cover 2094. The first receiving cavity 2091 and the second receiving cavity 2092 are both formed in the excitation base 2093. Each excitation dichroic mirror, each collimating lens, each excitation filter, a focusing cylindrical mirror 2028 and an excitation protective glass 211 are all inserted into the second receiving cavity 2092, which is conducive to improving the convenience of disassembly and assembly of the above-mentioned components, and is also conducive to realizing modular installation of the excitation box body 209, which can effectively improve the convenience of disassembly and assembly of the excitation box body 209.

[0061] In one embodiment of the present invention, the fluorescence detector 3 includes a detection box body 303 for accommodating the detection component 301 and the beam splitting component 302, and a plurality of detection components include a first detection component 3011, a second detection component 3012, a third detection component 3013, a fourth detection component 3014, a fifth detection component 3015, and a sixth detection component 3016, which are sequentially distributed along the longitudinal direction of the detection box body 303 and whose signal receiving sides are all facing the beam splitting component 302. Specifically, the first detection component 3011, the second detection component 3012, the third detection component 3013, the fourth detection component 3014, the fifth detection component 3015, and the sixth detection component 3016 are respectively arranged corresponding to the first light-emitting component 2011, the second light-emitting component 2012, the third light-emitting component 2013, the fourth light-emitting component 2014, the fifth light-emitting component 2015, and the sixth light-emitting component 2016; each of the above detection components can be selected as a fluorescence detector or a photodetector, which is used to receive the fluorescence signal and then convert the fluorescence signal into a photocurrent of corresponding linearity, and the photocurrent is converted into a photocurrent of corresponding linearity by a photocurrent. The processing is converted into a voltage output, and the numerical value of the voltage signal output after AD conversion can quantify the original fluorescence value; the controller 4 in this embodiment also includes a second control circuit board 402 which is communicatively connected to the first detection element 3011, the second detection element 3012, the third detection element 3013, the fourth detection element 3014, the fifth detection element 3015 and the sixth detection element 3016. This layout of each detection element facilitates wiring between it and the second control circuit board 402, simplifies the circuit layout, and facilitates the production and installation of the fluorescence detector 3 on an automated production line.

[0062] In this embodiment, if the sample reagent contains a fluorescent dye probe corresponding to ATTO 425 dye, a detection channel corresponding to the fluorescent dye probe is formed between the first detection element 3011 and the first light-emitting element 2011, that is, the fluorescent light beam excited by the excitation light beam emitted by the first light-emitting element 2011 is received by the first detection element 3011; if the sample reagent contains a fluorescent dye probe corresponding to FAM dye, a detection channel corresponding to the fluorescent dye probe is formed between the second detection element 3012 and the second light-emitting element 2012, that is, the fluorescent light beam excited by the excitation light beam emitted by the second light-emitting element 2012 is received by the second detection element 3012; if the sample reagent contains a fluorescent dye probe corresponding to HEX dye or VIC dye, a detection channel corresponding to the above-mentioned fluorescent dye probe is formed between the third detection element 3013 and the third light-emitting element 2013, that is, the fluorescent light beam excited by the excitation light beam emitted by the third light-emitting element 2013 is received by the third detection element 3013; If a fluorescent dye probe corresponding to ROX dye is included, a detection channel corresponding to the fluorescent dye probe is formed between the fourth detection element 3014 and the fourth light-emitting element 2014, that is, the fluorescent light beam excited by the excitation light beam emitted by the fourth light-emitting element 2014 is received by the fourth detection element 3014; if a fluorescent dye probe corresponding to CY5 dye is included in the sample reagent, a detection channel corresponding to the fluorescent dye probe is formed between the fifth detection element 3015 and the fifth light-emitting element 2015, that is, the fluorescent light beam excited by the excitation light beam emitted by the fifth light-emitting element 2015 is received by the fifth detection element 3015; if a fluorescent dye probe corresponding to Quasar705 or CY5.5 dye is included in the sample reagent, a detection channel corresponding to the fluorescent dye probe is formed between the sixth detection element 3016 and the sixth light-emitting element 2016, that is, the fluorescent light beam excited by the excitation light beam emitted by the sixth light-emitting element 2016 is received by the sixth detection element 3016;

[0063] If the sample reagent contains a fluorescent dye probe corresponding to the FRET dye, a detection channel corresponding to the fluorescent dye probe is formed between the third detection element 3013 and the second light-emitting element 2012, that is, the fluorescent light beam excited by the excitation light beam emitted by the second light-emitting element 2012 is received by the third detection element 3013; if the sample reagent contains a fluorescent dye probe corresponding to the AttoPhos dye, a detection channel corresponding to the fluorescent dye probe is formed between the third detection element 3013 and the first light-emitting element 2011, that is, the fluorescent light beam excited by the excitation light beam emitted by the first light-emitting element 2011 is received by the third detection element 3013; if the sample reagent contains a fluorescent dye probe corresponding to the SYPRO RED dye, a detection channel corresponding to the fluorescent dye probe is formed between the fifth detection element 3015 and the third light-emitting element 2013, that is, the fluorescent light beam excited by the excitation light beam emitted by the third light-emitting element 2013 is received by the fifth detection element 3015; if the sample reagent contains Super Bright 436 dye corresponding to the fluorescent dye probe, a detection channel corresponding to the fluorescent dye probe is formed between the fourth detection element 3014 and the first light-emitting element 2011, that is, the fluorescent light beam excited by the excitation light beam emitted by the first light-emitting element 2011 is received by the fourth detection element 3014.

[0064] In one embodiment of the present invention, a light inlet 304 facing the light emitting side 102 and used for receiving the fluorescent light beam is opened on one side wall of the detection box body 303, and the signal receiving side of the third detection member 3013 faces the light inlet 304; the beam splitting assembly 302 includes a first detection dichroic mirror 3021, a second detection dichroic mirror 3022, a third detection dichroic mirror 3023, a fourth detection dichroic mirror 3024, a fifth detection dichroic mirror 3025, a sixth detection dichroic mirror 3026, a seventh detection dichroic mirror 3027 and an emission cylindrical mirror 3028, and the first detection dichroic mirror 3021, the second detection dichroic mirror 3022 and the third detection dichroic mirror 3023 are arranged at intervals along the longitudinal direction of the detection box body 303 and are respectively located at the first detection member 3011, the second detection member 3013 2 and the signal receiving path of the third detection element 3013, the fourth detection dichroic mirror 3024, the fifth detection dichroic mirror 3025, the sixth detection dichroic mirror 3026 and the seventh detection dichroic mirror 3027 are arranged at intervals along the longitudinal direction of the detection box body 303 and are respectively located on the signal receiving paths of the fourth detection element 3014, the fifth detection element 3015, the sixth detection element 3016 and the third detection element 3013, the third detection dichroic mirror 3023 and the seventh detection dichroic mirror 3027 are arranged at intervals on the optical path of the third detection element 3013, and the emitting cylindrical mirror 3028 is arranged between the light inlet 304 and the seventh detection dichroic mirror 3027, and is used to disperse and collimate the fluorescent light beam injected along the light inlet 304 into parallel light, so as to improve the accuracy of the fluorescent light beam being emitted to each detection element;

[0065] The seventh detection dichroic mirror 3027 is used to reflect the fourth fluorescent light beam, the fifth fluorescent light beam, and the sixth fluorescent light beam corresponding to the fourth detection element 3014, the fifth detection element 3015, and the sixth detection element 3016 in the fluorescent light beam toward the fourth detection dichroic mirror 3024, the fifth detection dichroic mirror 3025, and the sixth detection dichroic mirror 3026, and is also used to transmit the first fluorescent light beam, the second fluorescent light beam, and the third fluorescent light beam corresponding to the first detection element 3011, the second detection element 3012, and the third detection element 3013 in the fluorescent light beam; the first detection dichroic mirror 3021 is used to reflect the first fluorescent light beam toward the first detection element 3011; The second detection dichroic mirror 3022 is used to reflect the second fluorescent beam toward the second detection component 3012 and transmit the first fluorescent beam; the third detection dichroic mirror 3023 is used to reflect the first fluorescent beam and the second fluorescent beam and transmit the third fluorescent beam to the third detection component 3013; the fourth detection dichroic mirror 3024 is used to reflect the fourth fluorescent beam toward the fourth detection component 3014 and transmit the fifth fluorescent beam and the sixth fluorescent beam; the fifth detection dichroic mirror 3025 is used to reflect the fifth fluorescent beam toward the fifth detection component 3015 and transmit the sixth fluorescent beam; the sixth detection dichroic mirror 3026 is used to reflect the sixth fluorescent beam toward the sixth detection component 3016. Through the reflection and / or transmission effect of the above-mentioned first detection dichroic mirror 3021, the second detection dichroic mirror 3022, the third detection dichroic mirror 3023, the fourth detection dichroic mirror 3024, the fifth detection dichroic mirror 3025, the sixth detection dichroic mirror 3026, and the seventh detection dichroic mirror 3027, the paths of the fluorescent light beams received by the first detection component 3011, the second detection component 3012, the third detection component 3013, the fourth detection component 3014, the fifth detection component 3015, and the sixth detection component 3016 are adjusted so that they can be accurately received by each detection component.

[0066] In one embodiment of the present invention, the fluorescence detector 3 further includes a first emission filter 306, a second emission filter 307, a third emission filter 308, a fourth emission filter 309, a fifth emission filter 310 and a sixth emission filter 311. The first emission filter 306 is arranged on the signal receiving side of the first detection element 3011 of the detection component 301 and is set to be a single bandpass filter with a central wavelength of 480nm and a bandwidth of 30nm. The second emission filter 307 is arranged on the signal receiving side of the second detection element 3012 of the detection component 301 and is set to be a single bandpass filter with a central wavelength of 520nm and a bandwidth of 20nm. The third emission filter 308 is arranged on the signal receiving side of the third detection element 3013 of the detection component 301 and is set to be a single bandpass filter with a central wavelength of 565nm and a bandwidth of 20nm. The fourth emission filter 309 is arranged on the signal receiving side of the fourth detection element 3014 of the detection component 301 and is set to a single bandpass filter with a central wavelength of 615nm and a bandwidth of 40nm. The fifth emission filter 310 is arranged on the signal receiving side of the fifth detection element 3015 of the detection component 301 and is set to a single bandpass filter with a central wavelength of 665nm and a bandwidth of 20nm. The sixth emission filter 311 is arranged on the signal receiving side of the sixth detection element 3016 of the detection component 301 and is set to a single bandpass filter with a central wavelength of 725nm and a bandwidth of 40nm. Through the filtering effect of each filter, the fluorescent light beam received by each detection element can accurately meet the specific wavelength requirements, which can effectively improve the accuracy and sensitivity of the fluorescence detector 3, which is conducive to further improving the detection accuracy and sensitivity of the fluorescence detection device.

[0067] In one embodiment of the present invention, the fluorescence detector 3 also includes an emission focusing assembly 312 disposed in the detection box body 303, and the emission focusing assembly 312 includes a first emission focusing mirror 3121, a second emission focusing mirror 3122, a third emission focusing mirror 3123, a fourth emission focusing mirror 3124, a fifth emission focusing mirror 3125 and a sixth emission focusing mirror 3126, wherein the first emission focusing mirror 3121 is disposed between the first detection member 3011 and the first emission filter 306, and is used to focus the fluorescence light beam received by the first detection member 3011; the second emission focusing mirror 3122 is disposed between the second detection member 3012 and the second emission filter 307, and is used to focus the fluorescence light beam received by the second detection member 3012. The third emission focusing mirror 3123 is arranged between the third detection member 3013 and the third emission filter 308, and is used for focusing the fluorescent light beam received by the third detection member 3013; the fourth emission focusing mirror 3124 is arranged between the fourth detection member 3014 and the fourth emission filter 309, and is used for focusing the fluorescent light beam received by the fourth detection member 3014; the fifth emission focusing mirror 3125 is arranged between the fifth detection member 3015 and the fifth emission filter 310, and is used for focusing the fluorescent light beam received by the fifth detection member 3015; the sixth emission focusing mirror 3126 is arranged between the sixth detection member 3016 and the sixth emission filter 311, and is used for focusing the fluorescent light beam received by the sixth detection member 3016.

[0068] In one embodiment of the present invention, the fluorescence detector 3 also includes an emission protection glass 305 arranged in the detection box body 303 and located between the emission cylindrical mirror 3028 and the light inlet 304. The emission protection glass 305 can extend the focal length and prevent the heat generated by the sample reagent in the reaction flat tube 1 when being heated from affecting the emission effect of the emission cylindrical mirror 3028.

[0069] In one embodiment of the present invention, a third accommodating chamber 3031 and a fourth accommodating chamber 3032 which are interconnected are provided in the detection box body 303; the first detection component 3011, the second detection component 3012, the third detection component 3013, the fourth detection component 3014, the fifth detection component 3015 and the sixth detection component 3016 are all accommodated in the third accommodating chamber 3031; each detection dichroic mirror, the first emission filter 306, the second emission filter 307, the third emission filter 308, the fourth emission filter 309, the fifth emission filter 310, the sixth emission filter 311, the emission cylindrical mirror 3028 and the emission protection glass 305 are all accommodated in the fourth accommodating chamber 3032; and the light inlet 304 is connected to the fourth accommodating chamber 3032. Specifically, the detection box body 303 includes a detachably connected detection base 3033 and a detection cover 3034, the third receiving cavity 3031 and the fourth receiving cavity 3032 are both formed in the detection base 3033, and each detection dichroic mirror, each emission filter, each emission focusing mirror, an emission cylindrical mirror 3028 and a detection protection glass are all inserted into the fourth receiving cavity 3032, which is conducive to improving the convenience of disassembly and assembly of the above-mentioned components, and is also conducive to realizing modular installation of the detection box body 303, which can effectively improve the convenience of disassembly and assembly of the detection box body 303.

[0070] In one embodiment of the present invention, the light-incoming side 101 and the light-outgoing side 102 are arranged at an angle of 90°, which can effectively reduce the background interference of the excitation light beam and the fluorescent light beam and improve the sensitivity of fluorescence detection.

[0071] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various specific technical features in any appropriate manner. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations. However, these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

[0072] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0073] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0074] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A fluorescence detection device, characterized in that: The fluorescence detection device comprises: A flat reaction tube (1) for storing sample reagents, wherein the sample reagents include a plurality of different types of fluorescent dye probes; A fluorescence exciter (2) is arranged on the light-incoming side (101) of the flat reaction tube (1) and comprises a light-emitting component (201) and a beam-combining component (202), wherein the light-emitting component (201) comprises a plurality of light-emitting components capable of respectively emitting excitation light beams of different colors, and the beam-combining component (202) is capable of adjusting the route of the excitation light beam and focusing the excitation light beam so that the excitation light beam is emitted to the sample reagent, and the fluorescent dye probe in the sample reagent is excited by the excitation light beam to generate a corresponding fluorescent light beam; A fluorescence detector (3) is arranged on the light-emitting side (102) of the reaction flat tube (1) and comprises a detection component (301) and a beam splitting component (302), wherein the detection component (301) comprises a plurality of detection components for detecting the fluorescence light beams of different wavelength bands, and the beam splitting component (302) is capable of splitting the fluorescence light beam and adjusting the path thereof so that the fluorescence light beam is directed to the corresponding detection component; The controller (4) is in communication with the fluorescence exciter (2) and the fluorescence detector (3) and is configured as follows: Determining the type of the fluorescent dye probe to be detected; Determining the color of the excitation light beam corresponding to the fluorescent dye probe according to the type; Controlling the light-emitting element corresponding to the fluorescent dye probe to emit light according to the color; Determining the wavelength band of the fluorescent light beam corresponding to the fluorescent dye probe according to the type; The detection element corresponding to the fluorescent dye probe is controlled to start the fluorescence detection function according to the waveband.

2. The fluorescence detection device according to claim 1, characterized in that: The plurality of light-emitting components include a first light-emitting component (2011), a second light-emitting component (2012), a third light-emitting component (2013), a fourth light-emitting component (2014), a fifth light-emitting component (2015), and a sixth light-emitting component (2016); the first light-emitting component (2011) is capable of emitting a purple excitation light beam, the second light-emitting component (2012) is capable of emitting a blue excitation light beam, the third light-emitting component (2013) is capable of emitting a green excitation light beam, the fourth light-emitting component (2014) is capable of emitting a yellow excitation light beam, the fifth light-emitting component (2015) is capable of emitting a red excitation light beam, and the sixth light-emitting component (2016) is capable of emitting a deep red excitation light beam.

3. The fluorescence detection device according to claim 2, characterized in that: The fluorescence exciter (2) further comprises a first excitation filter (203), a second excitation filter (204), a third excitation filter (205), a fourth excitation filter (206), a fifth excitation filter (207) and a sixth excitation filter (208), wherein the first excitation filter (203) is arranged on the light-emitting side of the first light-emitting element (2011) and is configured as a single bandpass filter with a central wavelength of 430 nm and a bandwidth of 24 nm, the second excitation filter (204) is arranged on the light-emitting side of the second light-emitting element (2012) and is configured as a single bandpass filter with a central wavelength of 480 nm and a bandwidth of 20 nm, the third excitation filter (205) is arranged on the light-emitting side of the second light-emitting element (2012) and is configured as a single bandpass filter with a central wavelength of 480 nm and a bandwidth of 20 nm, The third light-emitting element (2013) is disposed on the light-emitting side of the light-emitting element and is configured as a single bandpass filter with a central wavelength of 530 nm and a bandwidth of 20 nm. The fourth excitation filter (206) is disposed on the light-emitting side of the fourth light-emitting element (2014) and is configured as a single bandpass filter with a central wavelength of 570 nm and a bandwidth of 20 nm. The fifth excitation filter (207) is disposed on the light-emitting side of the fourth light-emitting element (2014) and is configured as a single bandpass filter with a central wavelength of 630 nm and a bandwidth of 20 nm. The sixth excitation filter (208) is disposed on the light-emitting side of the fourth light-emitting element (2014) and is configured as a single bandpass filter with a central wavelength of 682 nm and a bandwidth of 16 nm.

4. The fluorescence detection device according to claim 2, characterized in that: The fluorescence exciter (2) further comprises an excitation box body (209) for accommodating the light-emitting component (201) and the beam-combining component (202); the first light-emitting component (2011), the second light-emitting component (2012), the third light-emitting component (2013), the fourth light-emitting component (2014), the fifth light-emitting component (2015) and the sixth light-emitting component (2016) are sequentially distributed along the longitudinal direction of the excitation box body (209), and their respective light-emitting sides are all facing the beam-combining component (202).

5. The fluorescence detection device according to claim 4, characterized in that: The excitation box body (209) is formed with a light outlet (210) facing the light inlet side (101) on a side wall away from the light-emitting component (201), and the light-emitting side of the third light-emitting component (2013) faces the light outlet (210); the beam combining component (202) comprises a first excitation dichroic mirror (2021), a second excitation dichroic mirror (2022), a third excitation dichroic mirror (2023), a fourth excitation dichroic mirror (2024), a fifth excitation dichroic mirror (2025), a sixth excitation dichroic mirror (2026), a seventh excitation dichroic mirror (2027) and a focusing cylindrical mirror (2028), wherein the first excitation dichroic mirror (2021), the second excitation dichroic mirror (2022) and the third excitation dichroic mirror (2023) are arranged at intervals along the longitudinal direction of the excitation box body (209) and are respectively located at the corresponding positions. On the optical paths of the first light-emitting component (2011), the second light-emitting component (2012), and the third light-emitting component (2013), the fourth excitation dichroic mirror (2024), the fifth excitation dichroic mirror (2025), the sixth excitation dichroic mirror (2026), and the seventh excitation dichroic mirror (2027) are arranged side by side in the longitudinal direction of the excitation box body (209) and are respectively located on the optical paths of the fourth light-emitting component (2014), the fifth light-emitting component (2015), the sixth light-emitting component (2016), and the third light-emitting component (2013); the third excitation dichroic mirror (2023) and the seventh excitation dichroic mirror (2027) are arranged at intervals on the optical path of the third light-emitting component (2013); and the focusing cylindrical mirror (2028) is arranged between the seventh excitation dichroic mirror (2027) and the light outlet (210); The first excitation dichroic mirror (2021) is used to reflect the first excitation light beam emitted by the first light-emitting element (2011) toward the seventh excitation dichroic mirror (2027); the second excitation dichroic mirror (2022) is used to transmit the first excitation light beam and reflect the second excitation light beam emitted by the second light-emitting element (2012) toward the seventh excitation dichroic mirror (2027); the third excitation dichroic mirror (2023) is used to transmit the third excitation light beam emitted by the third light-emitting element (2013) and reflect the first excitation light beam and the second excitation light beam toward the seventh excitation dichroic mirror (2027); the sixth excitation dichroic mirror (2026) is used to reflect the sixth excitation light beam emitted by the sixth light-emitting element (2016) toward the seventh excitation dichroic mirror (2027); The light beam is directed toward the seventh excitation dichroic mirror (2027); the fifth excitation dichroic mirror (2025) is used to transmit the sixth excitation light beam and reflect the fifth excitation light beam emitted by the fifth light-emitting element (2015) toward the seventh excitation dichroic mirror (2027); the fourth excitation dichroic mirror (2024) is used to transmit the fifth excitation light beam and the sixth excitation light beam and reflect the fourth excitation light beam emitted by the fourth light-emitting element (2014) toward the seventh excitation dichroic mirror (2027); the seventh excitation dichroic mirror (2027) is used to transmit the first excitation light beam, the second excitation light beam, and the third excitation light beam and reflect the fourth excitation light beam, the fifth excitation light beam, and the sixth excitation light beam toward the light outlet (210).

6. The fluorescence detection device according to claim 5, characterized in that: The fluorescence exciter (2) further comprises an excitation protection glass (211) which is arranged in the excitation box body (209) and located between the focusing cylindrical mirror (2028) and the light outlet (210).

7. The fluorescence detection device according to claim 5, characterized in that: The excitation box body (209) is provided with a first receiving cavity (2091) and a second receiving cavity (2092) which are interconnected. The first light-emitting component (2011), the second light-emitting component (2012), the third light-emitting component (2013), the fourth light-emitting component (2014), the fifth light-emitting component (2015) and the sixth light-emitting component (2016) are all received in the first receiving cavity (2091). The first excitation dichroic mirror (221), the second excitation The dichroic mirror (2022), the third excitation dichroic mirror (2023), the fourth excitation dichroic mirror (2024), the fifth excitation dichroic mirror (2025), the sixth excitation dichroic mirror (2026), the seventh excitation dichroic mirror (2027), the focusing cylindrical mirror (2028) and the excitation protection glass (211) are all accommodated in the second accommodation cavity (2092), and the light outlet (210) is connected to the second accommodation cavity (2092).

8. The fluorescence detection device according to claim 1, characterized in that: The fluorescence detector (3) further comprises a first emission filter (306), a second emission filter (307), a third emission filter (308), a fourth emission filter (309), a fifth emission filter (310) and a sixth emission filter (311), wherein the first emission filter (306) is arranged at the signal receiving side of the first detection element (3011) of the detection component (301) and is configured as a single bandpass filter with a central wavelength of 480 nm and a bandwidth of 30 nm, the second emission filter (307) is arranged at the signal receiving side of the second detection element (3012) of the detection component (301) and is configured as a single bandpass filter with a central wavelength of 520 nm and a bandwidth of 20 nm, the third emission filter (308) is arranged at the signal receiving side of the second detection element (3012) of the detection component (301) and is configured as a single bandpass filter with a central wavelength of 520 nm and a bandwidth of 20 nm, and the third emission filter (309) is arranged at the signal receiving side of the first detection element (3011) of the detection component (301) and is configured as a single bandpass filter with a central wavelength of 520 nm and a bandwidth of 20 nm. The signal receiving side of the third detection element (3013) is set as a single bandpass filter with a central wavelength of 565 nm and a bandwidth of 20 nm. The fourth emission filter (309) is arranged on the signal receiving side of the fourth detection element (3014) of the detection component (301) and is set as a single bandpass filter with a central wavelength of 615 nm and a bandwidth of 40 nm. The fifth emission filter (310) is arranged on the signal receiving side of the fifth detection element (3015) of the detection component (301) and is set as a single bandpass filter with a central wavelength of 665 nm and a bandwidth of 20 nm. The sixth emission filter (311) is arranged on the signal receiving side of the sixth detection element (3016) of the detection component (301) and is set as a single bandpass filter with a central wavelength of 725 nm and a bandwidth of 40 nm.

9. The fluorescence detection device according to claim 4, characterized in that: The fluorescence detector (3) comprises a detection box body (303) for accommodating the detection assembly (301) and the beam splitting assembly (302), and the plurality of detection components comprise a first detection component (3011), a second detection component (3012), a third detection component (3013), a fourth detection component (3014), a fifth detection component (3015), and a sixth detection component (3016) which are sequentially distributed along the longitudinal direction of the detection box body (303) and each of which has its signal receiving side facing the beam splitting assembly (302).

10. The fluorescence detection device according to claim 9, characterized in that: The detection box body (303) is provided with a light inlet (304) on one side wall thereof, which is oriented toward the light emitting side (102) and is used to receive the fluorescent light beam. The signal receiving side of the third detection element (3013) faces the light inlet (304). The beam splitting assembly (302) comprises a first detection dichroic mirror (3021), a second detection dichroic mirror (3022), a third detection dichroic mirror (3023), a fourth detection dichroic mirror (3024), a fifth detection dichroic mirror (3025), a sixth detection dichroic mirror (3026), a seventh detection dichroic mirror (3027) and an emission cylindrical mirror (3028). The first detection dichroic mirror (3021), the second detection dichroic mirror (3022) and the third detection dichroic mirror (3023) are arranged at intervals along the longitudinal direction of the detection box body (303) and are respectively located at the first detection element (3013). (3011), the second detection member (3012) and the third detection member (3013) on the signal receiving path, the fourth detection dichroic mirror (3024), the fifth detection dichroic mirror (3025), the sixth detection dichroic mirror (3026) and the seventh detection dichroic mirror (3027) are arranged at intervals along the longitudinal direction of the detection box body (303) and are respectively located on the signal receiving paths of the fourth detection member (3014), the fifth detection member (3015), the sixth detection member (3016) and the third detection member (3013), the third detection dichroic mirror (3023) and the seventh detection dichroic mirror (3027) are arranged at intervals on the optical path of the third detection member (3013), and the emitting cylindrical mirror (3028) is arranged between the light inlet (304) and the seventh detection dichroic mirror (3027); The seventh detection dichroic mirror (3027) is used for reflecting the fourth fluorescent light beam, the fifth fluorescent light beam and the sixth fluorescent light beam in the fluorescent light beam respectively corresponding to the fourth detection element (3014), the fifth detection element (3015) and the sixth detection element (3016) toward the fourth detection dichroic mirror (3024), the fifth detection dichroic mirror (3025) and the sixth detection dichroic mirror (3026), and is also used for transmitting the first fluorescent light beam, the second fluorescent light beam and the third fluorescent light beam in the fluorescent light beam respectively corresponding to the first detection element (3011), the second detection element (3012) and the third detection element (3013); the first detection dichroic mirror (3021) is used for reflecting the first fluorescent light beam toward the first detection element (3011); the second The detection dichroic mirror (3022) is used to reflect the second fluorescent light beam toward the second detection element (3012) and transmit the first fluorescent light beam; the third detection dichroic mirror (3023) is used to reflect the first fluorescent light beam and the second fluorescent light beam and transmit the third fluorescent light beam to the third detection element (3013); the fourth detection dichroic mirror (3024) is used to reflect the fourth fluorescent light beam toward the fourth detection element (3014) and transmit the fifth fluorescent light beam and the sixth fluorescent light beam; the fifth detection dichroic mirror (3025) is used to reflect the fifth fluorescent light beam toward the fifth detection element (3015) and transmit the sixth fluorescent light beam; and the sixth detection dichroic mirror (3026) is used to reflect the sixth fluorescent light beam toward the sixth detection element (3016).

11. The fluorescence detection device according to claim 10, characterized in that: The fluorescence detector (3) further comprises an emission protection glass (305) which is arranged in the detection box body (303) and located between the emission cylindrical mirror (3028) and the light inlet (304).

12. The fluorescence detection device according to any one of claims 1 to 11, characterized in that: The light-incoming side (101) and the light-outgoing side (102) are arranged at an angle of 90°.

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