A method for producing tetraploid largemouth bass by inducing double hydrostatic shock

The fertilized eggs of largemouth bass were obtained through natural fertilization and treated with double hydrostatic shock method to successfully induce the formation of tetraploid largemouth bass, solving the problem of low success rate of tetraploid induction in the prior art, and achieving an efficient and simple tetraploid induction method.

CN119183996BActive Publication Date: 2025-03-18SOUTH CHINA AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202411732982.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-11-29
Publication Date
2025-03-18
Estimated Expiration
2044-11-29

AI Technical Summary

Technical Problem

The prior art is difficult to effectively induce tetraploids in largemouth bass, and the artificial insemination process is complex, and the quality and quantity of fertilized eggs are insufficient, resulting in a low success rate of tetraploid induction.

Method used

Natural fertilization was used to obtain fertilized eggs, and the fertilized eggs were treated by double hydrostatic shock method, which optimized the pressure intensity and treatment time, reduced damage to the fertilized egg, successfully inhibited the second cleavage, and induced the formation of tetraploid largemouth bass.

Benefits of technology

The tetraploid induction rate was improved to up to 24.44%, and the tolerance and hatching rate of fertilized eggs were significantly improved, the operation process was simplified, and the reproducibility and application value of the method were enhanced.

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Abstract

The present invention discloses a method for producing tetraploid largemouth black bass by inducing a double hydrostatic shock method. The method first obtains high-quality fertilized eggs through natural fertilization: the steps of injecting hormones and squeezing the abdomen to obtain sperm and eggs in the traditional method are avoided, thereby significantly reducing the damage to the parent fish. Combined with the innovative double hydrostatic shock method, by optimizing the pressure intensity and processing time, the damage to the fertilized eggs is further reduced, the second cleavage is successfully inhibited, and the tetraploid induction rate can be as high as 24.44%. The method can be applied to the tetraploid production of largemouth black bass: the operating method of the present invention is simple and easy, has strong repeatability, and has a high induction rate. These characteristics make the method of the present invention have a high application value in largemouth black bass breeding, and provide strong support for the breeding of tetraploid largemouth black bass.
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