Application of strigolactone derivative 7FGR24 in improvement of drought tolerance of crops

Abstract

The invention discloses an application of a strigolactone derivative 7FGR24 in improvement of drought tolerance of crops. The structural formula of the 7FGR24 is shown as a formula I in the specification. 7FGR24 is a plant endogenous hormone derivative, and is low in cost, green and harmless; the adopted treatment method is efficient, simple to operate, low in cost and suitable for large-scale application; and the drought resistance of the treated crops is obviously enhanced. # imgabs0 #

Description

Technical Field

[0001] The invention belongs to the technical field of crop cultivation, and particularly relates to application of a strigolactone derivative 7FGR24 in improving drought resistance of crops. Background Art

[0002] Drought is a major global adversity, which often leads to the production of a large number of reactive oxygen free radicals in crops, causing oxidative damage, reducing chlorophyll content, and further affecting crop growth and development, seriously restricting crop production and agricultural sustainable development. Therefore, improving crop drought tolerance is a bottleneck problem that needs to be solved urgently in modern agricultural production.

[0003] At present, there are two main methods to improve crop drought tolerance. One is to screen and create crop germplasm with strong drought tolerance through traditional or biotechnology breeding methods, so as to improve crop drought tolerance from the seed source; this method has limitations such as long cycle and high cost. The other is to enhance crop tolerance to drought through measures such as the application of growth regulators, so as to improve crop drought tolerance from the perspective of cultivation management; this method has the advantages of fast effect, low cost and wide applicability.

[0004] Strigolactone is a new type of plant growth regulator, which plays an important role in promoting plant root development and improving plant stress resistance. 7FGR24, its structural formula is as follows:

[0005] 7FGR24 is a new derivative of strigolactone. We have previously found that 7FGR24 has a good preservation effect on Gastrodia elata tubers (CN202110942027.2). There has been no relevant research or reports on its application in improving crop drought tolerance. Summary of the invention

[0006] One of the purposes of the present invention is to provide a new use of the strigolactone derivative 7FGR24.

[0007] The new use of the strigolactone derivative 7FGR24 provided by the present invention is the use of the strigolactone derivative 7FGR24 in improving the drought resistance of crops. The structural formula of the strigolactone derivative 7FGR24 is as follows: .

[0009] In the application, the crops include solanaceous and melon crops, specifically tomatoes and watermelons.

[0010] The second purpose of the present invention is to provide a method for improving the drought resistance of crops. The present invention uses the strigolactone derivative 7FGR24 to spray the crops on the leaves, thereby improving the drought resistance of crops in an efficient, simple and large-scale manner.

[0011] The method for improving drought tolerance of crops provided by the present invention is a Solanaceae crop, and the method comprises: spraying the Solanaceae seedlings with 7FGR24 aqueous solution on the leaves after the Solanaceae seedlings are transplanted to the three-leaf and one-heart stage; Wherein, the concentration of the 7FGR24 aqueous solution may be 0.001-20 μM, specifically 0.1-10 μM; Spray once every 5 to 10 days, specifically once every 5 to 7 days; Spray continuously 2 to 6 times, specifically 3 to 4 times.

[0012] The crop is a melon crop, and the method for improving the drought resistance of the crop is: after the melon seedlings are transplanted to the three-leaf and one-heart stage, the 7FGR24 aqueous solution is used to spray the melon seedlings with leaves; Wherein, the concentration of the 7FGR24 aqueous solution may be 0.01-20 μM, specifically 0.1-5 μM; Spray once every 3 to 10 days, specifically once every 4 to 9 days; Spray continuously 4 to 10 times, specifically 6 to 8 times.

[0013] The present invention adopts the strigolactone derivative 7FGR24 to spray the crops on the leaves, thereby enhancing the CAT and SOD activities in the crops and increasing the chlorophyll content, thereby improving the ability of the crops to resist antioxidant damage and photosynthesis, and further improving the tolerance of the crops to drought adversity and maintaining the normal growth of the crops.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The 7FGR24 used in the present invention is a plant endogenous hormone derivative, low in cost, green and harmless; the treatment method used is efficient, simple to operate, low in cost, and suitable for large-scale application; the drought resistance of the treated crops is significantly enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a comparison of the drought stress conditions of tomato plants treated with 7FGR24 and untreated tomato plants in Example 1 of the present invention.

[0016] Figure 2 This is a comparison of the drought stress conditions of the watermelon plants treated with 7FGR24 and the untreated watermelon plants in Example 2 of the present invention. DETAILED DESCRIPTION

[0017] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0018] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0019] The method disclosed in the present invention for improving drought tolerance of crops by using the strigolactone derivative 7FGR24 comprises: Ingredients and preparation: Add water to 7FGR24 according to the proportion to prepare an aqueous solution with a concentration of 100 mM.

[0020] Scope of application: Crops such as eggplants, melons, etc.

[0021] Directions: After the solanaceous seedlings are transplanted to the three-leaf and one-heart stage, dilute the above 7FGR24 aqueous solution to a concentration of 0.001-20 μM, and spray the leaves 2-6 times continuously, with an interval of 5-10 days. The dilution concentration can be 0.1-10 μM, the number of sprays can be 3-4 times, and the interval time can be 5-7 days.

[0022] After the melon seedlings are transplanted to the three-leaf and one-heart stage, dilute the 7FGR24 aqueous solution to a concentration of 0.01-20 μM, and spray the leaves 4-10 times continuously, with an interval of 3-10 days. The dilution concentration can be 0.1-5 μM, the number of sprays can be 6-8 times, and the interval time can be 4-9 days.

[0023] The 7FGR24 used in the present invention is a plant endogenous hormone derivative, low in cost, green and harmless; the treatment method used is efficient, simple to operate, low in cost, and suitable for large-scale application; the drought resistance of the treated crops is significantly enhanced.

[0024] Example 1 This embodiment provides a method for improving drought tolerance of tomatoes using the strigolactone derivative 7FGR24, comprising: 1) Preparation of 7FGR24 aqueous solution: Weigh 3.1706 g of 7FGR24 solid powder, dilute to 100 mL with deionized water, the concentration of the aqueous solution is 100 mM, and store at 4°C in dark place for later use; 2) Drought stress on tomato plants: Tomato seedlings at the cotyledon stage were taken and planted in a hydroponic box filled with 3 L of Hoagland nutrient solution for hydroponics. When the tomato seedlings grew to the three-leaf and one-heart stage, 300 g of PEG6000 was added to the nutrient solution to make the PEG6000 concentration in the nutrient solution 10%, and drought stress was applied; 3) Application of 7FGR24: After the tomato plants were stressed for 2 days, the 7FGR24 aqueous solution described in 1) was diluted from 100 mM to 1 μM, and the diluted 7FGR24 aqueous solution was evenly sprayed on the leaves of the tomato plants using a sprayer; spray once every 5 days for 4 consecutive times.

[0025] 4) Blank control: Spray the aqueous solution without 7FGR24 evenly on the leaves of tomato plants; spray once every 5 days for 4 consecutive times.

[0026] Figure 1 This is a comparison of the drought stress of tomato plants treated with 7FGR24 and those without treatment. Figure 1 It can be seen that the application of 7FGR24 can significantly improve the drought resistance of tomato plants.

[0027] Table 1. Comparison of tomato plant phenotypes and drought resistance physiological indicators after 7FGR24 treatment

[0028] Example 2 This embodiment provides a method for improving drought resistance of watermelon using the strigolactone derivative 7FGR24, comprising: 1) Preparation of 7FGR24 aqueous solution: Weigh 1.5853 g of 7FGR24 solid powder, dilute to 100 mL with deionized water, the concentration of the aqueous solution is 50 mM, and store at 4°C in dark place for later use; 2) Drought stress on watermelon plants: watermelon seedlings at the cotyledon stage were taken and planted in a hydroponic box filled with 3 L of Hoagland nutrient solution for hydroponics. When the watermelon seedlings grew to the three-leaf and one-heart stage, 300 g of PEG6000 was added to the nutrient solution to make the PEG6000 concentration in the nutrient solution 10%, and drought stress was applied; 3) Application of 7FGR24: After the watermelon plants were stressed for 2 days, the 7FGR24 aqueous solution described in 1) was diluted from 50 mM to 0.1 μM, and the diluted 7FGR24 aqueous solution was evenly sprayed on the leaves of the watermelon plants using a sprayer; spraying was performed once every 4 days for a total of 7 times.

[0029] 4) Blank control: Spray the aqueous solution without 7FGR24 evenly on the leaves of watermelon plants; spray once every 4 days, for a total of 7 times.

[0030] 5) GR24 application control: The aqueous solution containing 0.1 μM GR24 was evenly sprayed on the leaves of watermelon plants; spray once every 4 days, and spray 7 times.

[0031] Figure 2 This is a comparison of the drought stress of watermelon plants treated with 7FGR24 and those without treatment. Figure 2 It can be seen that the application of 7FGR24 can significantly improve the drought resistance of watermelon plants.

[0032] It can be seen from the data in Table 2 that 7FGR24 is more effective in improving the drought tolerance of watermelon plants than the standard strigolactone GR24.

[0033] Table 2. Comparison of phenotypes and drought resistance physiological indicators of watermelon plants after treatment with 7FGR24 and GR24

[0034] The present invention has been described in detail above. For those skilled in the art, without departing from the purpose and scope of the present invention, and without the need to carry out unnecessary experimental conditions, the present invention can be implemented in a wide range under equivalent parameters, concentrations and conditions. Although the present invention provides specific embodiments, it should be understood that the present invention can be further improved. In a word, according to the principles of the present invention, the application is intended to include any changes, uses or improvements to the present invention, including departure from the disclosed scope in the application, and changes made with conventional techniques known in the art.

Claims

1. Application of the strigolactone derivative 7FGR24 in improving crop drought tolerance. The structural formula of the strigolactone derivative 7FGR24 is shown below: 。 2. The use according to claim 1, characterized in that: The crops include solanaceous and melon crops.

3. The use according to claim 1 or 2, characterized in that: The crops are tomatoes and watermelons.

4. A method for improving drought tolerance of crops, wherein the crops are Solanaceae crops, and the method comprises: spraying the Solanaceae seedlings with 7FGR24 aqueous solution on the leaves after the Solanaceae seedlings are transplanted until they reach the three-leaf and one-heart stage.

5. The method according to claim 4, characterized in that The concentration of the 7FGR24 aqueous solution is 0.001-20 μM; it is sprayed once every 5-10 days; and it is sprayed continuously for 2-6 times.

6. The method according to claim 5, characterized in that The concentration of the 7FGR24 aqueous solution is 0.1-10 μM; it is sprayed once every 5-7 days; and it is sprayed continuously for 3-4 times.

7. A method for improving drought tolerance of crops, wherein the crops are melon crops, and the method comprises: spraying the melon seedlings with 7FGR24 aqueous solution on the leaves after the melon seedlings are transplanted until they reach the three-leaf and one-heart stage.

8. The method according to claim 7, characterized in that The concentration of the 7FGR24 aqueous solution is 0.01-20 μM; it is sprayed once every 3-10 days; and it is sprayed continuously for 4-10 times.

9. The method according to claim 8, characterized in that The concentration of the 7FGR24 aqueous solution is 0.1-5 μM; it is sprayed once every 4-9 days, and sprayed continuously for 6-8 times.

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