Carrier preparation method for improving stability and solubility of lactic acid bacteria silage leavening agent

Abstract

The invention relates to the technical field of silage fermentation, and discloses a carrier preparation method for improving stability and solubility of a lactic acid bacteria silage leavening agent, which comprises the following steps: S1, feeding carrier powder into a fluidized bed through a vacuum pipeline; s2, pouring an adhesive into an adhesive slurry tank through a double-layer screen for later use; s3, starting air inlet and heating of the fluidized bed to boil the material and raise the temperature, when the material reaches 50-52 DEG C, ensuring that the adhesive is stably sprayed out in a mist form, and adjusting the flow velocity of the adhesive according to the material condition; s4, drying and sterilizing after the state of the carrier particles meets the requirement; s5, after sterilization is completed, the materials are discharged into a vibrating screen, and undersize materials are collected for standby application. Carrier particles are formed after carrier powder is granulated and dried through a fluidized bed, the carrier particles are used as the lactic acid bacteria silage leavening agent carrier, the viable count reduction of the silage leavening agent in the storage and use process can be effectively relieved, and the silage leavening agent can be effectively prevented from being damaged. Strain stability is enhanced, rapid dissolution of the silage leavening agent is promoted, and a spraying device is prevented from being blocked.

Description

Technical Field

[0001] The invention relates to the technical field of silage fermentation, and in particular to a method for preparing a carrier capable of improving the stability and solubility of a lactic acid bacteria silage starter. Background Art

[0002] Silage is an important feed source in animal husbandry. It is made by fermenting fresh plants such as corn stalks and grass under anaerobic conditions, so that they can be preserved for a long time and maintain a certain nutritional value.

[0003] Adding lactic acid bacteria silage starter during the ensilage process can promote silage fermentation. Lactic acid bacteria can quickly produce acid, inhibit mold and yeast, and reduce nutrient loss. The effect of silage starter is not only affected by the strain formula and the number of viable bacteria, but also related to the strain composition, strain stability during storage and transportation, and the method of use. The choice of carrier for silage starter will affect the attenuation of the number of viable bacteria during storage, and a suitable carrier can delay the decrease in the number of viable bacteria. The carrier is also an important factor affecting the solubility of silage starter. Silage starter that can dissolve quickly can not only improve work efficiency, but also effectively prevent the silage starter from clogging the spraying device.

[0004] After being mixed with lactic acid bacteria, traditional carriers dissolve slowly. Carriers such as starch have poor dispersibility in water and require a long time of stirring to completely dissolve. Precipitation is easy to occur after dissolution, which can easily clog the device during spraying and fail to spray the lactic acid bacteria evenly into the silage raw materials. Microbial activity is also affected by the type and physical properties of the carrier. Inappropriate carriers lead to poor stability of the strain and unstable fermentation activity, which not only affects the efficiency of silage fermentation, but may also cause problems with local uneven fermentation. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method for preparing a carrier for improving the stability and solubility of lactic acid bacteria silage starter, thereby solving the problems of slow dissolution speed and poor solubility, which affect the uniform spraying of the silage starter in the silage raw materials, and the microorganisms in the carrier are easily disturbed by the external environment, resulting in poor stability of the strain and unstable fermentation activity.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] A method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter, comprising the following steps:

[0008] S1. Loading carrier powder into the fluidized bed through a vacuum pipe;

[0009] S2, pour the adhesive through a double-layer sieve into an adhesive slurry tank for standby use;

[0010] S3, the fluidized bed starts air intake and heating to make the material boil and heat up. When the material reaches 50-52°C, the peristaltic pump is turned on to ensure that the adhesive is sprayed out stably in the form of mist. The flow rate of the adhesive is adjusted according to the material conditions. During the process, the material temperature is controlled at 50-55°C. During the production process, samples are taken for particle size detection to observe the state of the carrier particles;

[0011] S4. After the state of the carrier particles meets the requirements, they are dried and sterilized, the air inlet temperature is adjusted to heat the material, and sterilization is performed;

[0012] S5. After sterilization, the materials are discharged into the vibrating screen and the screened materials are collected for later use.

[0013] Preferably, in S2, the double-layer sieve particle size is 110-130 mesh, and the carrier powder is maltodextrin or 90% maltodextrin + 10% aluminosilicate powder.

[0014] Preferably, in S2, the binder is pure water, and the amount of pure water used is 30-40 L of pure water per 200 kg of carrier powder.

[0015] Preferably, in S4, the inlet air temperature is adjusted to raise the temperature of the material to 98° C., and the sterilization treatment is maintained for 20 minutes.

[0016] Preferably, in S5, after screening using a 30-mesh vibrating screen, the screened material is collected for later use.

[0017] Preferably, the carrier particles have a moisture content of ≤1% and a water activity of ≤0.1.

[0018] Preferably, the carrier particles are used to prepare silage starter or other mixed feed additives.

[0019] The present invention provides a method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter. The method has the following beneficial effects:

[0020] 1. The present invention uses a fluidized bed to granulate and dry the carrier powder to form the carrier particles. Using the carrier particles as a carrier of lactic acid bacteria silage starter can effectively slow down the reduction of the number of live bacteria in the silage starter during storage and use, enhance the stability of the strain, promote the rapid dissolution of the silage starter, and avoid clogging of the spraying device.

[0021] 2. The carrier particles prepared by the method of the present invention can effectively improve the solubility and strain stability of the silage starter. After mixing with lactic acid bacteria, the loose structure of the particles can dissolve faster in water.

[0022] 3. When the moisture content of the carrier particles of the present invention is controlled at ≤1% and the water activity is ≤0.1, the stability of the silage starter and the mixed feed additive can be greatly improved. The low moisture content and low water activity environment effectively inhibits the growth and reproduction of microorganisms, and reduces the risk of product deterioration due to microbial activity. This enables the starter and feed additive to maintain their quality and activity during storage and transportation, prolongs the shelf life of the product, and reduces the economic losses caused by product deterioration. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0024] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Please see attached Figure 1 A method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter, characterized in that it comprises the following steps:

[0026] S1. Loading carrier powder into the fluidized bed through a vacuum pipe;

[0027] S2, pour the pure water through a double-layer sieve into the adhesive slurry tank for standby use;

[0028] S3. The fluidized bed is turned on for air intake and heating to make the material boil and heat up. When the material reaches 50-52°C, the peristaltic pump is turned on to ensure that the adhesive is sprayed out in a mist-like and stable manner. The flow rate of the adhesive is adjusted according to the material conditions. During the process, the material temperature is controlled at 50-55°C. During the production process, samples are taken for particle size testing to observe the product status.

[0029] S4. After the state of the carrier particles meets the requirements, they are dried and sterilized, the air inlet temperature is adjusted to heat the material, and sterilization is performed;

[0030] S5. After sterilization, the material is discharged into the vibrating screen and the material under the screen is collected for later use.

[0031] In S2, the binder is pure water, and the amount of pure water used is 30-40L of pure water per 200kg of carrier powder.

[0032] Specifically, pure water can dissolve and disperse the various components in the carrier powder very well, so that they can be evenly combined together during the granulation process. Different carrier powders may have different water absorption and particle sizes during the granulation process. For carrier powders with strong water absorption, the amount of pure water can be appropriately reduced to avoid the particles being too wet; for carrier powders with weaker water absorption, the amount of pure water can be appropriately increased to ensure that the particles can be fully combined.

[0033] In S4, the inlet air temperature is adjusted to raise the material temperature to 98°C and the sterilization treatment is maintained for 20 minutes.

[0034] Specifically, a high temperature of 98°C can effectively kill various microorganisms that may exist in the material, including bacteria, fungi, and viruses. It is very important to ensure the quality and safety of the starter, and avoid affecting the fermentation effect or causing silage to deteriorate due to microbial contamination. Different microorganisms have different tolerance to high temperatures. Some microorganisms may take a long time to be completely killed. Maintaining a temperature of 98°C for 20 minutes can ensure that most microorganisms are effectively killed, thereby achieving a good sterilization effect. During the sterilization process, the moisture in the material will continue to evaporate, and the adhesive in the particles will gradually solidify. Maintaining it for 20 minutes allows the particles to have enough time to dry and solidify, thereby improving the quality and stability of the particles.

[0035] In S5, after sieving using a 30-mesh vibrating screen, the sieve underflow is collected for later use.

[0036] Specifically, the sieve hole size of the 30-mesh vibrating screen can effectively control the size of the material particles. Through screening, oversized particles can be removed to obtain a particle size range that meets the requirements. This is very important for ensuring the performance and quality of the fermentation agent, because the fermentation agent with uniform particle size is easier to mix evenly with the silage raw materials during use, thereby improving the fermentation effect.

[0037] The moisture content of the carrier particles is ≤1%, and the water activity is ≤0.1.

[0038] Specifically, the moisture content of the carrier particles is controlled at ≤1%. Having a low moisture content can effectively prevent the growth and reproduction of microorganisms. The growth of microorganisms usually requires a certain moisture environment. When the moisture content is low, they are difficult to survive and metabolize, thereby ensuring the stability and quality of the starter. Secondly, low moisture content helps prevent the carrier particles from agglomerating during storage and transportation. If the moisture content is too high, the particles are prone to stick to each other, affecting their fluidity and performance. In addition, low moisture content can also extend the shelf life of the starter and reduce chemical changes and quality degradation caused by moisture.

[0039] The carrier particles are used to prepare silage starter or other mixed feed additives.

[0040] Specifically, the carrier particles can protect the microorganisms from the influence of the external environment, such as drying, high temperature, oxidation, etc. In the preparation process of silage starter, by controlling the moisture content and water activity of the carrier particles, the metabolic activity of the microorganisms can be reduced, making them in a relatively stable state, thereby extending the shelf life of the starter.

[0041] The following is an introduction in conjunction with specific embodiments:

[0042] Embodiment 1:

[0043] This embodiment provides a silage starter using maltodextrin particles and aluminosilicate as carriers, wherein the components include 70 parts of carrier particles and 30 parts of lactic acid bacteria powder, wherein the lactic acid bacteria powder is composed of Lactobacillus plantarum and Lactobacillus buchneri, and the number of viable bacteria in the silage starter is 1.0×10 11 CFU / g;

[0044] S1, maltodextrin powder is fed into the fluidized bed through a vacuum pipe;

[0045] S2. Pour 30-40L / 200kg of maltodextrin powder of pure water through a double-layer 120-mesh sieve into the adhesive slurry tank for later use.

[0046] S3. The fluidized bed starts air intake and heating to make the material boil and heat up. When the material reaches 50-52°C, the peristaltic pump is turned on to ensure that the adhesive is sprayed out stably in the form of mist. The flow rate of the adhesive is adjusted according to the material conditions. The material temperature is controlled at 50-55°C during the process. During the production process, samples are taken for particle size testing to observe the product status.

[0047] S4. After the particle state meets the requirements, it is dried and sterilized. When the inlet air temperature is adjusted, the material temperature reaches 98°C and is maintained for 20 minutes for sterilization.

[0048] S5. After sterilization, the material is discharged into the vibrating screen, and the material under the 30-mesh screen is collected for later use.

[0049] S6. The prepared maltodextrin granules are evenly mixed with lactic acid bacteria powder to obtain a silage starter.

[0050] Example 2: Compared with Example 1, the difference is that the silage starter component is 60 parts of carrier particles (90% maltodextrin + 10% aluminosilicate), 40 parts of lactic acid bacteria powder, the lactic acid bacteria powder is composed of plant lactobacillus, and the number of viable bacteria in the silage starter is 1.2×10 11 CFU / g.

[0051] Comparative Example 1: Its components are 70 parts of maltodextrin powder and 30 parts of lactic acid bacteria powder, wherein the lactic acid bacteria powder is composed of Lactobacillus plantarum and Lactobacillus buchneri, and the number of viable bacteria in the silage starter is 1.0×1011 CFU / g, maltodextrin powder is fed into a fluidized bed through a vacuum pipe, the fluidized bed is opened for air intake and heating to make the material boil and heat up, the air intake temperature is adjusted to make the material temperature reach 98°C, and the material is kept for 20 minutes for drying and sterilization. The moisture content of the obtained dried maltodextrin powder is ≤1%, and the water activity is ≤0.1. The prepared dried maltodextrin powder is evenly mixed with lactic acid bacteria powder to obtain a silage starter.

[0052] The difference between Comparative Example 2 and Example 1 is that the carrier is glucose.

[0053] In order to verify the storage stability of the carrier particles of the invention example on the lactic acid bacteria in the lactic acid bacteria silage starter and the rapid solubility of the silage starter, the following test was conducted:

[0054] 1. Test methods

[0055] (1) The experiment set up the following 4 treatment groups:

[0056] Test group 1: silage starter prepared in Example 1.

[0057] Test group 2: silage starter prepared in Example 2.

[0058] Experimental group 3: silage fermentation agent prepared in comparative example 1.

[0059] Experimental Group 4: silage fermentation agent prepared in Comparative Example 2.

[0060] (2) Weigh 400 g of sample from each group of silage fermentation agent, pack each group into 4 aluminum foil bags, each bag weighing 100 g. After exhausting the air, seal the bags with a heat sealer and check for air leakage. Put the samples into a constant temperature and humidity chamber with the temperature set to 37°C and the humidity set to 60%. Detect the viable count and survival rate on the 0th, 15th, 30th and 60th days respectively. The viable count detection method shall be implemented in accordance with GB4789.35.

[0061] Experimental results:

[0062]

[0063] The experimental results show that:

[0064] As time went on, on the 15th, 30th and 60th days, the number of live bacteria in each embodiment gradually decreased, and the survival rate also decreased accordingly. However, the preservation rate of the silage fermentation agent in the embodiment using carrier particles was significantly better than that of the embodiment using maltodextrin powder and glucose; similar results were obtained for the survival rate of the strains at each time point. On the 60th day, the survival rate of the test group 4 dropped to 30%, while the survival rates of the test groups 1 and 2 were still higher than 70%, which reflected the gradual loss of microbial activity during long-term storage, but also showed that the preparation method of the fermentation agent carrier can maintain the activity of the microorganism to a certain extent, so that it still maintains a relatively high presence of live bacteria within 60 days, thereby ensuring its effectiveness in the silage fermentation process.

[0065] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter, characterized in that: The following steps are involved: S1. Loading carrier powder into the fluidized bed through a vacuum pipe; S2, pour the adhesive through a double-layer sieve into an adhesive slurry tank for standby use; S3, the fluidized bed starts air intake and heating to make the material boil and heat up. When the material reaches 50-52°C, the peristaltic pump is turned on to ensure that the adhesive is sprayed out stably in the form of mist. The flow rate of the adhesive is adjusted according to the material conditions. During the process, the material temperature is controlled at 50-55°C. During the production process, samples are taken for particle size detection to observe the state of the carrier particles; S4. After the state of the carrier particles meets the requirements, they are dried and sterilized, the air inlet temperature is adjusted to heat the material, and sterilization is performed; S5. After sterilization, the material is discharged into the vibrating screen and the material under the screen is collected for later use.

2. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: In S2, the particle size of the double-layer sieve is 110-130 mesh, and the carrier powder is maltodextrin or 90% maltodextrin + 10% aluminosilicate powder.

3. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: In S2, the binder is pure water, and the amount of pure water used is 30-40L of pure water per 200kg of carrier powder.

4. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: In S4, the inlet air temperature is adjusted to raise the material temperature to 98°C and the sterilization treatment is maintained for 20 minutes.

5. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: In S5, after screening using a 30-mesh vibrating screen, the screened material is collected for later use.

6. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: The moisture content of the carrier particles is ≤1%, and the water activity is ≤0.

1.

7. The method for preparing a carrier for improving the stability and solubility of a lactic acid bacteria silage starter according to claim 1, characterized in that: The carrier particles are used for preparing silage starter or other mixed feed additives.

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