Dendrobium officinale mixture and preparation method thereof

Abstract

The invention belongs to the technical field of food processing, and particularly relates to a dendrobium officinale mixture and a preparation method thereof, the mixture comprises the following raw materials: dendrobium officinale, a drying aid and plant polyphenol; the drying aid comprises cellulose ether. The dendrobium officinale mixture provided by the invention can be quickly dissolved, has no caking, and is good in dispersity and solubility, various nutrient substances of dendrobium officinale are well reserved, the powder yield is high, the polysaccharide content is high, and a blended beverage is light green in color and mellow and smooth in taste.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and particularly relates to a dendrobium officinale mixture and a preparation method thereof. Background Art

[0002] Dendrobium candidum is a perennial herb. It has been called "the holy product of nourishing yin" since ancient times. It has the effects of nourishing yin and promoting body fluid, warming the stomach and moistening the lungs, and preventing cancer and aging. It is now a national key protected medicinal material variety. Dendrobium candidum contains rich nutritional active substances, including polysaccharides, alkaloids, phenols, bibenzyls, terpenes, flavonoids, etc. According to modern medical experiments, Dendrobium candidum has multiple effects such as anti-tumor, anti-inflammatory, lowering blood pressure, lowering blood sugar, nourishing yin and moistening dryness, strengthening the spleen and appetizing. With the revival of the traditional Chinese medicine health care craze, people are increasingly aware of the value of Dendrobium candidum, which has rapidly developed the Dendrobium industry, and there are more and more related processed products, which has improved the use value of Dendrobium in traditional eating methods. Dendrobium candidum powder has become the mainstream product form because of its convenience and comprehensive nutrition. However, due to its rich polysaccharide content, the powder is very easy to aggregate into a mass when taken with water, and it is difficult to disperse, which seriously affects consumers' consumption. Therefore, it is of great significance to develop instant Dendrobium candidum mixture products. Summary of the invention

[0003] The object of the present invention is to provide a dendrobium officinale mixture and a preparation method thereof. The dendrobium officinale mixture has good solubility and high nutritional value, and the prepared beverage has rich taste and emerald green color.

[0004] The invention provides a dendrobium officinale mixture. The raw materials of the mixture include: dendrobium officinale, a drying aid and plant polyphenols; the drying aid includes cellulose ether.

[0005] Preferably, the cellulose ether includes one or more of methyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, hydroxyethyl cellulose and hydroxyethyl methyl cellulose.

[0006] Preferably, the Dendrobium officinale is ground by wet method to prepare Dendrobium officinale suspension.

[0007] Preferably, in the wet grinding, the mass volume ratio of Dendrobium officinale to water is 1 g: 1 to 20 mL.

[0008] Preferably, the added mass of the drying aid is 5% to 15% of the total mass of the Dendrobium officinale suspension.

[0009] Preferably, the added mass of the plant polyphenols is 0.3% to 1.2% of the total mass of the Dendrobium officinale suspension.

[0010] Preferably, the plant polyphenols include one or more of tannic acid, gallic acid, tea polyphenols, catechins, ferulic acid and caffeic acid.

[0011] The present invention provides a method for preparing the above-mentioned Dendrobium officinale mixture, comprising the following steps:

[0012] The Dendrobium officinale is mixed with water and ground to obtain a Dendrobium officinale suspension;

[0013] The Dendrobium officinale suspension, the drying aid and the plant polyphenols are mixed, homogenized and dried to obtain the Dendrobium officinale mixture.

[0014] Preferably, the grinding temperature is 0-10° C.; the grinding gear spacing is 10-200 μm; and the grinding times are 1-5 times.

[0015] Preferably, the homogenization pressure is 20 to 50 MPa; and the homogenization is performed 1 to 4 times.

[0016] Beneficial effects: The present invention provides a Dendrobium officinale mixture, the raw materials of the mixture include: Dendrobium officinale, a drying aid and plant polyphenols; the drying aid includes cellulose ether. The present invention selects cellulose ether with good film-forming properties as a drying aid, and utilizes the spontaneous interaction between polyphenols and cellulose ether to encapsulate Dendrobium polysaccharides and other beneficial ingredients, thereby avoiding thermal degradation of the beneficial ingredients and improving the rehydration of the powder. The Dendrobium officinale mixture provided by the present invention can be quickly dissolved, has no agglomeration, is well dispersed, and has good solubility. Various nutrients of Dendrobium officinale are well retained, the powder yield is high, the polysaccharide content is high, and the prepared beverage has a tender green color and a mellow and smooth taste.

[0017] The present invention provides a method for preparing the above-mentioned Dendrobium officinale mixture, which does not require concentration and granulation, so as to solve the technical problems of low utilization rate of effective ingredients of Dendrobium officinale and difficulty in rehydration in the prior art. At present, the preparation method of instant powder of Dendrobium officinale on the market is mainly to extract and concentrate from Dendrobium officinale to obtain Dendrobium polysaccharide, and then granulate and dry. However, the extraction and concentration process not only consumes a lot of time, but also loses some active ingredients; and the granulation process has cumbersome steps and may also involve organic reagents. The present invention utilizes wet grinding to effectively extract and retain active substances including Dendrobium polysaccharide, dendrobium alkaloids, phenols, bibenzyls, terpenes, etc. by controlling the water ratio and gear spacing, fully extracting the nutrients in Dendrobium, improving the bioavailability of raw materials, and no further concentration is required. The present invention utilizes the hydrogen bond interaction between cellulose ether and plant polyphenols to change and regulate the particle size and internal structure. First, cellulose ether is uniformly dispersed in a suspension of Dendrobium officinale as a drying aid under high pressure homogenization, and then polyphenol is added. Based on the interaction between the two, cellulose ether will agglomerate into nuclei to form agglomerates of different sizes, and finally, after high temperature drying by a spray-drying atomizer, instant granules with uniform size and internal voids are generated. The preparation method provided by the present invention is simple to operate, does not require concentration and granulation, simplifies the steps, avoids the pollution of organic reagents, retains various nutrients of Dendrobium officinale to the greatest extent, and effectively improves the solubility of the Dendrobium officinale mixture, and can be instantized in warm water for 3 to 18 seconds. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The Dendrobium officinale mixture and the reconstituted Dendrobium officinale beverage prepared in Example 1;

[0019] Figure 2 The polysaccharide standard curve. DETAILED DESCRIPTION

[0020] The invention provides a dendrobium officinale mixture. The raw materials of the mixture include: dendrobium officinale, a drying aid and plant polyphenols; the drying aid includes cellulose ether.

[0021] Unless otherwise specified, the present invention has no special requirements for the preparation raw materials, and commercially available products known to those skilled in the art can be used.

[0022] The Dendrobium officinale of the present invention preferably includes: a Dendrobium officinale suspension.

[0023] The present invention preferably pre-treats Dendrobium candidum, and the pre-treatment preferably includes: washing, cutting, and boiling water color protection. The washing of the present invention preferably uses clean water. The cutting of the present invention preferably cuts Dendrobium candidum into small segments of 1 to 3 cm, more preferably 2 cm. The boiling water color protection of the present invention preferably puts Dendrobium candidum into boiling water containing a color protection agent. The color protection agent of the present invention preferably includes: one or more of sodium chloride, ascorbic acid, sodium ascorbate, isoascorbic acid and sodium isoascorbate, more preferably sodium chloride or sodium isoascorbate. The added mass of the color protection agent of the present invention is preferably 0.03% to 0.06% of the total mass of water, more preferably 0.03%. The mass volume ratio of Dendrobium candidum and water of the present invention is preferably 0.5 to 1.5 g: 40 to 60 mL, more preferably 1 g: 50 mL. The time of boiling water color protection of the present invention is 60 to 90 s, more preferably 60 s or 90 s. After the boiling water color protection is completed, the method is immediately cooled to obtain the pre-treated Dendrobium officinale.

[0024] The present invention preferably uses wet grinding to prepare a Dendrobium officinale suspension by the above-mentioned pre-treated Dendrobium officinale, that is, mixing the Dendrobium officinale with water and grinding to obtain the Dendrobium officinale suspension. The mass volume ratio of the Dendrobium officinale to water is preferably 1g:1-20mL, more preferably 1g:20mL or 1g:7-10mL or 1g:5-7mL or 1g:3-5mL or 1g:1-3mL. The grinding temperature of the present invention is preferably 0-10°C, more preferably 0-4°C. The present invention adopts low-temperature grinding to obtain the Dendrobium suspension. Compared with the traditional solvent extraction method, the amount of solvent (water) used is less. With the assistance of mechanical grinding, relatively less water can effectively obtain the effective ingredients in the fresh Dendrobium officinale strips, without the need for subsequent concentration to remove water. The gear spacing of the grinding of the present invention is preferably 10 to 200 μm; more preferably 150 to 200 μm or 100 to 150 μm or 50 to 100 μm or 10 to 50 μm. The gear spacing of the present invention is regulated by the coarseness adjustment rod of the rotary colloid mill, and the active substances including dendrobium polysaccharides, dendrobium alkaloids, phenols, bibenzyls, terpenes, etc. are effectively extracted and retained by controlling the water ratio and the gear spacing, thereby improving the bioavailability of the raw materials. The number of grindings of the present invention is preferably 1 to 5 times, more preferably 2 to 4 times, and most preferably 3 times. The present invention preferably grinds and then filters to obtain a turbid suspension, and mixes multiple turbid suspensions to obtain a Dendrobium officinale suspension. The filter press of the present invention is preferably a belt filter press, and the number of filter presses is preferably 1 to 3 times, more preferably 2 times, and each time is preferably 2 to 5 minutes, more preferably 3 minutes. When the grinding described in the present invention is implemented, the parameters need to be slightly adjusted each time. Taking grinding three times as an example, it is preferred that the Dendrobium officinale and water are added to the colloid mill with a solid-liquid ratio of 1g:7-10mL, the temperature is controlled at 0-10°C, the grinding size is set to 150-200μm, the running time is 5-10min, and after the grinding is completed, a turbid suspension A is obtained by filter pressing; the filter residue repeats the above operation again, the solid-liquid ratio is 1g:5-7mL, the temperature is controlled at 0-10°C, the grinding size is set to 100-150μm, the running time is 5-10min, and after the grinding is completed, a turbid suspension B is obtained by filter pressing; then, the filter residue is collected and ground again, the solid-liquid ratio is 1g:3-5mL, the temperature is controlled at 0-10°C, the grinding size is set to 50-100μm, the running time is 5-10min, and after the grinding is completed, a turbid suspension C is obtained by filter pressing, and finally the suspensions A, B and C are combined to obtain a Dendrobium officinale suspension. The invention utilizes a colloid mill to perform multiple grinding and filter pressing to obtain a suspension of dendrobium officinale, and fully extracts the nutrients in the dendrobium by adjusting the water ratio and the distance between the grinding gears.

[0025] The present invention preferably mixes the Dendrobium officinale suspension with a flavoring agent for flavoring. The flavoring agent of the present invention preferably includes: a sweetener and / or an acidulant. The sweetener of the present invention preferably includes: one or more of lactose, white sugar and fructose syrup, more preferably lactose or white sugar. The added mass of the sweetener of the present invention is preferably 2% to 5% of the total mass of the Dendrobium officinale suspension, more preferably 3%. The acidulant of the present invention preferably includes citric acid and / or malic acid, more preferably citric acid. The added mass of the acidulant of the present invention is preferably 0.02% to 0.3% of the total mass of the Dendrobium officinale suspension, more preferably 0.05%.

[0026] The present invention preferably mixes the suspension of Dendrobium officinale with a drying aid to complete the preparation. The drying aid of the present invention preferably includes cellulose ether, and a cellulose ether with good film-forming property is selected as the drying aid. The cellulose ether of the present invention preferably includes: one or more of methyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, hydroxyethyl cellulose and hydroxyethyl methyl cellulose, more preferably methyl cellulose, hydroxyethyl cellulose or hydroxypropyl cellulose. The added mass of the drying aid of the present invention is preferably 5% to 15% of the total mass of the suspension of Dendrobium officinale, more preferably 8% to 12%, and most preferably 10%. The suspension of Dendrobium officinale of the present invention is rich in polysaccharides and has a relatively high viscosity. After drying, the powder particles are too fine and have a high water content. The powder is easily aggregated and difficult to disperse when taken with water; the addition of the drying aid helps to increase the size of the powder particles, reduce the moisture content, increase the product yield, and improve the solubility of the powder to a certain extent.

[0027] The present invention preferably homogenizes the prepared Dendrobium officinale suspension. The homogenization pressure of the present invention is preferably 20 to 50 MPa, more preferably 30 to 40 MPa, and most preferably 30 MPa. The number of homogenizations of the present invention is preferably 1 to 4 times, more preferably 2 to 3 times, and most preferably 2 times. The present invention adds a drying aid before homogenization, and the high-pressure homogenization process helps the drying aid to be more evenly dispersed in the Dendrobium officinale suspension.

[0028] The present invention preferably adds plant polyphenols to the homogenized Dendrobium officinale suspension and stirs evenly. The plant polyphenols of the present invention preferably include: one or more of tannic acid, gallic acid, tea polyphenols, catechins, ferulic acid and caffeic acid, more preferably tannic acid, gallic acid or tea polyphenols. The added mass of the plant polyphenols of the present invention is preferably 0.3% to 1.2% of the total mass of the Dendrobium officinale suspension, more preferably 0.8% to 1.0%. The present invention utilizes plant polyphenols as a cross-linking agent, and based on the interaction between polyphenols and cellulose ethers, the cellulose ethers uniformly dispersed in the Dendrobium suspension are induced to aggregate and nucleate, and then the Dendrobium polysaccharides are embedded to improve the rehydration of the powder.

[0029] The present invention preferably performs drying after homogenization, and the drying is preferably spray drying. The spray drying conditions of the present invention are: the inlet temperature is preferably 150-170°C, more preferably 160-170°C; the outlet temperature is preferably 80-90°C, more preferably 80 or 90°C; the flow rate is preferably 0.4-0.6L / h, more preferably 0.6L / h. The present invention finally passes through a spray-dried atomizer, and after high-temperature drying, instant granules with uniform size and internal voids are generated, which replaces the granulation process, simplifies the steps, and avoids the contamination of organic reagents, making the obtained product safer and healthier.

[0030] The present invention preferably obtains a Dendrobium officinale mixture after the above drying, and the beverage prepared by the Dendrobium officinale mixture has a rich taste, emerald green color, good solubility and high nutritional value. The mixture of the present invention can be dissolved in warm water for 3 to 18 seconds, the temperature of the warm water is preferably 40 to 80° C., and the mass ratio of the mixture to water is preferably 0.5 to 1.5:10 to 30, more preferably 1:20.

[0031] The mixture of the present invention can be used to prepare Dendrobium officinale powder, and can further be used to prepare Dendrobium officinale solid beverage and liquid beverage.

[0032] In order to further illustrate the present invention, a Dendrobium officinale mixture and a preparation method thereof provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0033] Example 1: One-step wet grinding

[0034] (1) Weigh 10 g of fresh Dendrobium officinale strips, clean them, cut them into 2 cm long segments, add them to 1 L boiling water containing 0.3 g sodium chloride and keep them warm for 90 seconds, then immediately remove them and cool them. Add the fresh Dendrobium officinale segments to 200 mL ice water (0°C), set the gear spacing to 150 μm, grind for 5 minutes, and then separate them by belt filter press for 3 minutes, twice, and filter press to obtain Dendrobium officinale suspension.

[0035] (2) 3% (w / w) white sugar as a sweetener, 0.05% (w / w) citric acid as an acidulant, and 10% (w / w) methylcellulose as a drying aid were added to the Dendrobium officinale suspension, and after being stirred evenly, the prepared Dendrobium officinale suspension was subjected to high-pressure homogenization at a homogenization pressure of 30 MPa for 2 times. Subsequently, 0.8% (w / w) tannic acid was slowly added under continuous stirring to agglomerate the methylcellulose into nuclei, followed by high-speed shearing at a speed of 6000 rpm for 2 minutes to ensure that the system was uniform and free of large aggregates, thereby obtaining a Dendrobium officinale mixed liquid.

[0036] (3) spray drying the Dendrobium officinale mixed solution in step (2) under the following conditions: inlet temperature of 170° C., outlet temperature of 80° C., and flow rate of 0.6 L / h to obtain a Dendrobium officinale mixture.

[0037] Example 2: Three wet grinding

[0038] (1) Weigh 10g of fresh Dendrobium officinale strips, clean them, cut them into small pieces of 3cm in length, add them to 1L boiling water containing 0.3g sodium isoascorbate, keep them warm for 90s, and then immediately remove them and cool them. Add the fresh Dendrobium officinale segments to 100mL ice water (0℃), set the gear spacing to 150μm, grind for 5min, and then separate them by belt filter press for 3min, 2 times, and filter press to obtain Dendrobium officinale suspension A. Collect the Dendrobium officinale residue and add it back to 60mL ice water, set the gear spacing to 100μm, grind for 5min, and then filter press to obtain Dendrobium officinale suspension B. Collect the residue again and add it to 40mL ice water, set the gear spacing to 50μm, grind for 5min, and then filter press to obtain Dendrobium officinale suspension C. Mix Dendrobium officinale suspension A, Dendrobium officinale suspension B, and Dendrobium officinale suspension C to obtain Dendrobium officinale suspension.

[0039] (2) 3% (w / w) lactose as a sweetener, 0.05% (w / w) citric acid as an acidulant, and 10% (w / w) methylcellulose as a drying aid were added to the Dendrobium officinale suspension, and after being stirred evenly, the prepared Dendrobium officinale suspension was subjected to high-pressure homogenization at a homogenization pressure of 30 MPa for 2 times. Subsequently, 0.8% (w / w) tannic acid was slowly added under continuous stirring to agglomerate the methylcellulose into nuclei, followed by high-speed shearing at a speed of 6000 rpm for 2 minutes to ensure that the system was uniform and free of large aggregates, thereby obtaining a Dendrobium officinale mixed liquid.

[0040] (3) spray drying the Dendrobium officinale mixed solution in step (2) under the following conditions: inlet temperature 160° C., outlet temperature 90° C., flow rate 0.6 L / h, to obtain a Dendrobium officinale mixture.

[0041] Example 3: Hydroxypropyl cellulose

[0042] (1) Weigh 10g of fresh Dendrobium officinale strips, clean them, cut them into small pieces of 3cm in length, add them to 1L boiling water containing 0.3g sodium isoascorbate, keep them warm for 90s, and then immediately remove them and cool them. Add the fresh Dendrobium officinale segments to 100mL ice water (0℃), set the gear spacing to 150μm, grind for 5min, and then separate them by belt filter press for 3min, 2 times, and filter press to obtain Dendrobium officinale suspension A. Collect the Dendrobium officinale residue and add it back to 70mL ice water, set the gear spacing to 100μm, grind for 5min, and then filter press to obtain Dendrobium officinale suspension B. Collect the residue again and add it to 30mL ice water, set the gear spacing to 50μm, grind for 5min, and then filter press to obtain Dendrobium officinale suspension C. Dendrobium officinale suspension A, Dendrobium officinale suspension B, and Dendrobium officinale suspension C are mixed to obtain Dendrobium officinale suspension.

[0043] (2) 3% (w / w) lactose as a sweetener, 0.05% (w / w) citric acid as an acidulant, and 10% (w / w) hydroxypropyl cellulose as a drying aid were added to the Dendrobium officinale suspension, and after being stirred evenly, the prepared Dendrobium officinale suspension was subjected to high-pressure homogenization treatment at a homogenization pressure of 30 MPa for 2 times. Subsequently, 0.8% (w / w) tannic acid was slowly added under continuous stirring to agglomerate the hydroxypropyl cellulose into nuclei, followed by high-speed shearing at a speed of 6000 rpm for 2 minutes to ensure that the system was uniform and free of large aggregates, thereby obtaining a Dendrobium officinale mixed liquid.

[0044] (3) spray drying the Dendrobium officinale mixed solution in step (2) under the following conditions: inlet temperature 160° C., outlet temperature 80° C., flow rate 0.6 L / h, to obtain a Dendrobium officinale mixture.

[0045] Example 4: Hydroxyethyl Cellulose

[0046] The process was carried out in the same manner as in Example 3, except that 10% (w / w) hydroxyethyl cellulose was used as a drying aid.

[0047] Example 5: Gallic acid

[0048] The method of Example 3 was followed, except that (1) the mixture was cut into small pieces of 2 cm in length and kept in boiling water for 90 seconds; (2) 10% (w / w) methyl cellulose was used as a drying agent, and 0.8% gallic acid was added.

[0049] Example 6: Tea polyphenols

[0050] The method of Example 3 was followed, except that (1) the mixture was kept warm in boiling water for 90 seconds; (2) 10% (w / w) methyl cellulose was used as a drying agent, and 0.8% (w / w) tea polyphenols were added.

[0051] Example 7: High concentration of methylcellulose

[0052] The process was carried out in the same manner as in Example 2, except that 15% (w / w) methylcellulose was used as a drying aid.

[0053] Example 8: Low concentration of methylcellulose

[0054] The process was carried out in the same manner as in Example 2, except that 5% (w / w) methylcellulose was used as a drying aid.

[0055] Example 9: Tannic acid low concentration

[0056] The process was carried out in the same manner as in Example 2, except that 0.3% (w / w) tannic acid was added.

[0057] Comparative Example 1: No drying agent added

[0058] The method of Example 2 was followed, except that no drying aid was added and 1.5% (w / w) tannic acid was added.

[0059] Comparative Example 2: Extraction once + no polyphenols

[0060] The method of Example 1 was followed, except that no plant polyphenols were added after homogenization, and spray drying was performed directly.

[0061] Test Example 1

[0062] The following method was used to prepare the Dendrobium officinale mixture (solid powder and its dissolved solution, see Figure 1 ) were tested, and three parallel tests were performed to obtain the average value. The results were recorded in Tables 1 and 2.

[0063] 1. Powder yield: Collect the powdered oil in the cyclone separator and collector and weigh them, and calculate the yield. The calculation formula is as follows:

[0064]

[0065] 2. Polysaccharide content: Accurately weigh 0.5g of sample powder and place it in a 100mL conical flask. Use 30mL of deionized water to extract at 80℃ with stirring for 1h, then centrifuge at 5000r / min for 15min. After extracting three times using the above method, combine the extracts and place them in an evaporating dish for evaporation and concentration. After the extract is concentrated, add three times the volume of anhydrous ethanol to precipitate overnight, then centrifuge at 5000r / min for 15min, collect the polysaccharide precipitate, evaporate the ethanol, and make up to 100mL to obtain the sample polysaccharide extract.

[0066] Measurement: Phenol-sulfuric acid method is used. Dry the glucose standard at 105°C to constant weight, then weigh 0.10g and dilute to 100mL to obtain a 1.00mg / mL glucose standard solution. Pipette 0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, and 0.5mL of the standard solution into a 10mL colorimetric tube, add water to 1mL, and slowly add 1mL of 5% (w / v) phenol solution (fresh) and 5mL of concentrated sulfuric acid in sequence, shake well, let stand for 5min, heat in a 30°C water bath for 15min, and then measure the absorbance at 490nm. Take 1mL of the sample extract, determine the sample absorbance according to the above method, and use the standard curve (see Figure 2 ) Calculate the polysaccharide content in the sample.

[0067] 3. Total phenol content: The total phenol content was measured by the Folin phenol method. 1 mL of the supernatant of the centrifuged Dendrobium officinale suspension was taken (4°C, 5000 rpm, 10 min), 1 mL of Folin phenol reagent was added, followed by 3 mL of 7.5% sodium carbonate solution and 5 mL of deionized water. The mixture was mixed thoroughly and allowed to stand in a dark place for 2 h. After the reaction was completed, the absorbance was measured at 760 nm. The result was expressed as gallic acid equivalent (mg GAE / L).

[0068] 4. Total flavonoids content: Take 1 mL of the centrifugal supernatant of the Dendrobium officinale suspension (4°C, 5000 rpm, 10 min), add 4 mL of deionized water and 0.3 mL of 5% sodium nitrite solution, add 0.3 mL of 10% aluminum nitrate solution after 5 minutes and mix, let it stand for 10 min. Then add 1 mL of 1 mol / L sodium hydroxide solution, make up to 10 mL with deionized water, measure the absorbance at a wavelength of 510 nm, and the result is expressed as rutin equivalent (mg RE / L).

[0069] 5. Chlorophyll content: Determine according to "NY / T 3082-2017 Spectrophotometric method for determination of chlorophyll content in fruits, vegetables and their products".

[0070] 6. Dissolution time: Disperse 1.0 g of the obtained solid powder of the mixture in 20 mL of warm water at 60°C, stir slowly, and record the time for complete dissolution.

[0071] 7. Dissolution state: Observe the dissolution state of the dissolved Dendrobium officinale drink with the naked eye.

[0072] Table 1 Effects of different grinding times on total phenols, total flavonoids and chlorophyll in Dendrobium officinale suspension

[0073]

[0074] Note: Letters a and b indicate significant differences between data in the same column.

[0075] As can be seen from Table 1, the three wet grinding processes used in Example 2 resulted in total phenols, total flavonoids and chlorophyll contents in the Dendrobium officinale suspension being 1.73 times, 1.99 times and 1.83 times those in Example 1, respectively. It can be seen that the three wet grindings effectively increased the active ingredients in the Dendrobium officinale suspension. This is because the three wet grinding process is not just repeated grinding three times, but the grinding size is adjusted during the process, the grinding size gradually becomes smaller, and the Dendrobium officinale fiber is gradually crushed, making it easier for active substances such as total phenols to be released and separated into water.

[0076] Table 2 Test results of beverages with different treatment methods

[0077]

[0078] As can be seen from Table 2, the difference between Examples 1 and 2 lies in the number of grinding and filtration presses. The more grinding times (Example 2), the higher the polysaccharide content. The difference between Examples 2, 7 and 8 lies in the amount of methylcellulose added. The amount of methylcellulose added in Example 7 is low, resulting in a slightly poor effect of embedding dendrobium polysaccharides, and then the powder rehydration has small lumps. The amount of methylcellulose added in Example 8 is high, which makes the embedding effect of dendrobium polysaccharides better and the powder dissolution time faster, but the proportion of polysaccharides will be reduced. The difference between Examples 2, 3, and 4 lies in the different types of cellulose ethers, but all can effectively embed dendrobium polysaccharides. The amount of tannic acid added in Example 9 is low, which also leads to a slightly poor embedding effect of dendrobium polysaccharides and reduces the solubility of the powder. The difference between Examples 2, 5, and 6 lies in the difference in plant polyphenols. Tannic acid is more likely to react with cellulose ethers because it contains more active groups. In Comparative Example 1, no drying aid was added, which made it difficult to spray dry, had aggregation, and dispersed very slowly when dissolved; in Comparative Example 2, no polyphenol treatment was performed, which made it very easy for the dendrobium polysaccharide to aggregate after direct drying.

[0079] It can be seen that the present invention is simple to operate, does not require concentration and granulation, retains various nutrients of Dendrobium officinale to the greatest extent, and the prepared Dendrobium officinale composition has good solubility, and the prepared beverage has a tender green color (see Figure 1 ), with a mellow and smooth taste.

[0080] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A Dendrobium officinale mixture, characterized in that: The raw materials of the mixture include: dendrobium officinale, a drying aid and plant polyphenols; the drying aid includes cellulose ether.

2. The mixture according to claim 1, characterized in that The cellulose ether includes one or more of methyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, hydroxyethyl cellulose and hydroxyethyl methyl cellulose.

3. The mixture according to claim 1, characterized in that The Dendrobium officinale is ground by wet method to prepare Dendrobium officinale suspension.

4. The mixture according to claim 3, characterized in that In the wet grinding, the mass volume ratio of Dendrobium officinale to water is 1g:1-20mL.

5. The mixture according to claim 3, characterized in that The added mass of the drying aid is 5% to 15% of the total mass of the Dendrobium officinale suspension.

6. The mixture according to claim 3, characterized in that The added mass of the plant polyphenols is 0.3% to 1.2% of the total mass of the Dendrobium officinale suspension.

7. The mixture according to claim 1, characterized in that The plant polyphenols include one or more of tannic acid, gallic acid, tea polyphenols, catechins, ferulic acid and caffeic acid.

8. The method for preparing the Dendrobium officinale mixture according to any one of claims 1 to 7, characterized in that: The following steps are involved: The Dendrobium officinale is mixed with water and ground to obtain a Dendrobium officinale suspension; The Dendrobium officinale suspension, the drying aid and the plant polyphenols are mixed, homogenized and dried to obtain the Dendrobium officinale mixture.

9. The preparation method according to claim 8, characterized in that: The grinding temperature is 0-10° C.; the grinding gear spacing is 10-200 μm; and the grinding times is 1-4 times.

10. The preparation method according to claim 8, characterized in that: The homogenization pressure is 20-50 MPa; the homogenization times are 1-5 times.

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