Skin care essential oil with moisturizing, anti-aging and pore refining effects as well as preparation method and application of skin care essential oil

Abstract

The invention relates to skin care essential oil with moisturizing, anti-aging and pore refining effects as well as a preparation method and application of the skin care essential oil. The skin care essential oil is prepared from components as follows: cape jasmine flower essential oil nano-liposome, lemon essential oil, rosemary essential oil, tea tree oil, beta-glucan, coenzyme Q10 and polyol. The cape jasmine flower essential oil is creatively loaded in the nano-liposome, so that the skin feeling of the cape jasmine flower essential oil is improved, the greasy and heavy feeling is reduced, the cape jasmine flower essential oil can be protected from being easily oxidized and deteriorated, the efficacy stability of the cape jasmine flower essential oil is improved, and the permeability and bioavailability of the cape jasmine flower essential oil can be improved; and the skin-care product can be more easily absorbed and utilized by the skin. Meanwhile, the gardenia essential oil nano-liposome is compounded with lemon essential oil, rosemary essential oil, tea tree oil, beta-glucan, coenzyme Q10 and polyhydric alcohol, and the functional components are matched with one another, so that the final essential oil product has excellent moisturizing, anti-aging and pore refining effects and excellent skin feeling and shelf stability.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and relates to a skin care essential oil with moisturizing, anti-aging and pore-refining effects, and a preparation method and application thereof. Background Art

[0002] As people's living standards continue to improve, consumers have higher and higher requirements for daily beauty and skin care products. Natural plant essential oil skin care products are loved by more and more consumers. More and more skin care products play skin care effects by adding plant essential oils. There are two main types of essential oils in skin care products: flower and herbal essential oils and Chinese herbal essential oils. Among them, Chinese herbal essential oils have specific effects, bad smells, and are more irritating to the skin. They are rarely added to skin care products as skin care essential oils; flower and herbal essential oils have a fresh smell and are less irritating. They are more easily accepted by consumers and are widely used in skin care products. Moreover, most of the essential oil products on the market currently have a single effect, the effect is not significant enough, and they cannot protect and repair the skin in all directions.

[0003] Gardenia oil is rich in a variety of antioxidant ingredients, such as linalool, camphene, caryophyllene, geraniol, etc. It can be used to apply to the skin to increase the skin's antioxidant capacity, remove free radicals, reduce free radical damage to the skin, and thus delay the aging process of skin cells. Gardenia oil also has the function of moisturizing and repairing the skin. It can quickly penetrate into the stratum corneum of the skin and form a thin film on the surface of the skin, helping the skin to maintain moisture balance, keep the skin moist, make the skin firmer and more elastic, and better resist aging. At the same time, gardenia oil also has excellent cell regeneration ability, which can repair dry, fragile, sensitive skin and the pits and scars left by acne skin, and restore the skin to a healthy state.

[0004] Although gardenia oil has the above advantages, it has the disadvantages of being greasy, heavy, and having a poor skin feel when applied directly to the skin. It will increase the burden on the skin and may also damage the structure of the sebum membrane, causing clogged pores and oily face. In addition, gardenia oil contains a large number of unsaturated bonds, which are easily oxidized by oxygen in the air, resulting in changes in the molecular formula, that is, the rancidity of the plant oil. Therefore, gardenia oil will easily deteriorate if left for a long time, making it difficult to effectively play a skin care effect. Instead, it will irritate human skin and increase skin allergy. At the same time, gardenia oil contains saturated fatty acids. When the ambient temperature is below zero, the plant oil will easily become solid, which seriously affects the experience of using it.

[0005] Therefore, it is very meaningful to develop a skin care product containing gardenia essential oil that can overcome the above-mentioned disadvantages of gardenia oil and achieve multi-faceted and multi-functional skin care effects. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide a skin care essential oil with moisturizing, anti-aging and pore-refining effects, and a preparation method and application thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a skin care essential oil with moisturizing, anti-aging and pore-refining effects, wherein the components of the skin care essential oil include: gardenia essential oil nanoliposomes, lemon essential oil, rosemary essential oil, tea tree oil, β-glucan, coenzyme Q10 and polyols.

[0009] The present invention creatively loads gardenia essential oil in nanoliposomes, not only improves the skin feel of gardenia essential oil, reduces greasy and heavy feeling, can also protect gardenia essential oil from being difficult to be oxidized and deteriorate, improves its efficacy stability, can also improve the permeability and bioavailability of gardenia essential oil, make it easier to be absorbed and utilized by the skin. Simultaneously, the gardenia essential oil nanoliposomes are compounded with lemon essential oil, rosemary essential oil, tea tree oil, beta-glucan, coenzyme Q10 and polyol, wherein beta-glucan can promote the percutaneous absorption of gardenia essential oil in coordination with liposomes, further promote the percutaneous absorption of other active components, and can also improve the moisturizing skin care effect of gardenia essential oil. Wherein lemon essential oil, rosemary essential oil, tea tree oil three-combination can further improve the skin feel of gardenia essential oil, can also promote each other in coordination with gardenia essential oil in the effects of moisturizing, anti-aging and delicate pores. Among them, coenzyme Q10 can protect the gardenia essential oil from being easily oxidized and deteriorated, improve its efficacy stability, and can also synergize with the gardenia essential oil to improve the anti-aging effect of the product. The above-mentioned functional components cooperate with each other, so that the final essential oil product has excellent moisturizing, anti-aging and pore-refining effects, as well as excellent skin feel and shelf stability.

[0010] Preferably, the components of the skin care essential oil include, by weight: 25-35 parts of gardenia essential oil nanoliposomes, 5-15 parts of lemon essential oil, 5-15 parts of rosemary essential oil, 60-80 parts of tea tree oil, 1-10 parts of β-glucan, 0.1-5 parts of coenzyme Q10 and 5-30 parts of polyols.

[0011] Based on the potential synergistic effect between the above-mentioned active ingredients, when they are combined in the above-mentioned specific mass ratio, the effects of skin care essential oils in moisturizing, anti-aging and pore-refining are more significant.

[0012] The weight proportions of the gardenia essential oil nanoliposomes can be selected to be 25 parts, 27 parts, 28 parts, 29 parts, 30 parts, 32 parts, 33 parts, 34 parts, 35 parts, etc.

[0013] The weight proportions of the lemon essential oil can be selected to be 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.

[0014] The weight proportions of the rosemary essential oil can be selected to be 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.

[0015] The weight proportions of the tea tree oil can be 60 parts, 62 parts, 64 parts, 66 parts, 68 parts, 70 parts, 72 parts, 75 parts, 80 parts, etc.

[0016] The weight proportions of the β-glucan can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, etc.

[0017] The weight proportion of the coenzyme Q10 can be selected from 0.2 parts, 0.3 parts, 0.6 parts, 0.8 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, etc.

[0018] The weight proportions of the polyol can be 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 20 parts, 22 parts, 25 parts, 30 parts, etc.

[0019] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0020] Preferably, the gardenia essential oil nanoliposomes are prepared by a preparation method comprising the following steps:

[0021] (1) mixing and dissolving phospholipids, cholesterol and gardenia essential oil in an organic solvent to form an oil phase liquid;

[0022] (2) removing the organic solvent from the oil phase by rotary evaporation and drying to form a dry lipid film;

[0023] (3) mixing the lipid film with a buffer and a surfactant, and ultrasonically dispersing the mixture to form an emulsion;

[0024] (4) centrifuging the emulsion to remove the upper essential oil and the lower impurities, and filtering to obtain the gardenia essential oil nanoliposomes.

[0025] Preferably, the mass ratio of the phospholipid, cholesterol and gardenia essential oil is (3-8):(1-2):1.

[0026] The specific point values ​​in (3-8) can be selected from 3, 4, 5, 6, 7, 8, etc.; the specific point values ​​in (1-2) can be selected from 1, 1.2, 1.4, 1.6, 1.8, 2, etc.; other specific point values ​​within the above numerical ranges can be selected and will not be listed here one by one.

[0027] Preferably, the phospholipids include soybean lecithin or egg yolk lecithin.

[0028] Preferably, the rotary evaporation is carried out at 35-50°C, such as 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, 45°C, 50°C, etc.

[0029] Preferably, the drying is carried out under vacuum at 50-65°C (e.g., 50°C, 52°C, 53°C, 55°C, 57°C, 60°C, 62°C, 64°C, 65°C, etc.) for 12-30h (e.g., 12h, 15h, 17h, 18h, 20h, 22h, 25h, 30h, etc.).

[0030] Preferably, the mass ratio of the lipid film, buffer and surfactant is (1-10):(2-20):1.

[0031] The specific point values ​​in (1-10) can be selected from 1, 2, 3, 4, 5, 7, 8, 9, 10, etc.; the specific point values ​​in (2-20) can be selected from 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, etc.

[0032] Preferably, the buffer comprises phosphate buffer.

[0033] Preferably, the surfactant comprises polyvinyl pyrrolidone or sodium carboxymethyl cellulose.

[0034] Preferably, the ultrasonic dispersion is carried out in a water bath at 50-65°C (e.g., 50°C, 52°C, 54°C, 55°C, 58°C, 60°C, 62°C, 63°C, 65°C, etc.).

[0035] Preferably, the centrifugation is performed at 5000-7000 rpm (e.g., 5000 rpm, 5300 rpm, 5500 rpm, 6000 rpm, 6500 rpm, 7000 rpm, etc.) for 10-60 min (e.g., 10 min, 20 min, 30 min, 40 min, 50 min, 60 min, etc.).

[0036] Preferably, the filtration is performed using a 0.22 μm microporous filter membrane.

[0037] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0038] Preferably, the polyol includes any one of ethylene glycol, propylene glycol, butylene glycol, pentanediol, hexanediol, glycerol, pentaerythritol or sorbitol, or a combination of at least two thereof.

[0039] Further preferably, the components of the skin care essential oil also include: any one of squalane, vitamin E or resveratrol, or a combination of at least two of them.

[0040] Vitamin E is further added to the skin care essential oil formula involved in the present invention, which can cooperate with coenzyme Q10 to protect the gardenia essential oil from being easily oxidized and deteriorated, further improve its efficacy stability, and similarly synergistically improve the anti-aging effect of the product with coenzyme Q10 and gardenia essential oil.

[0041] Squalane or resveratrol is further added to the skin care essential oil formula of the present invention, and the two can synergistically further improve the moisturizing and anti-aging effects of the skin care essential oil.

[0042] Preferably, the components of the skin care essential oil further include, by weight, any one of 2-16 parts of squalane, 0.02-0.2 parts of vitamin E or 1-1.5 parts of resveratrol, or a combination of at least two of them.

[0043] The weight proportion of the squalane can be 2 parts, 4 parts, 6 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts, etc.; the weight proportion of the vitamin E can be 0.02 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.15 parts, 0.18 parts, 0.2 parts, etc.; the weight proportion of the resveratrol can be 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, etc.

[0044] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0045] In a second aspect, the present invention provides a method for preparing the skin care essential oil having moisturizing, anti-aging and pore-refining effects according to the first aspect, the preparation method comprising the following steps:

[0046] (1) Heat tea tree oil and then mix with lemon essential oil and rosemary essential oil;

[0047] (2) mixing with β-glucan, coenzyme Q10, and polyol and cooling;

[0048] (3) mixing with gardenia essential oil liposomes to obtain the skin care essential oil.

[0049] Preferably, the tea tree oil is heated to 40-55°C, for example 40°C, 42°C, 44°C, 45°C, 48°C, 50°C, 52°C, 54°C, 55°C, etc.

[0050] Preferably, step (2) further comprises mixing with any one of squalane, vitamin E or resveratrol, or a combination of at least two thereof.

[0051] Preferably, the cooling in step (2) is to 5-15°C, for example 5°C, 6°C, 7°C, 8°C, 10°C, 11°C, 12°C, 13°C, 14°C, 15°C, etc.

[0052] Preferably, the mixing in step (3) is followed by filtering.

[0053] In a third aspect, the present invention provides use of the skin care essential oil according to the first aspect in preparing a product with moisturizing, anti-aging and pore-refining effects.

[0054] Preferably, the product includes essential oil products, lotions, emulsions, essences, face creams, facial masks, make-up removal products or skin care products.

[0055] Compared with the prior art, the present invention has the following beneficial effects:

[0056] The present invention creatively loads gardenia essential oil in nanoliposomes, not only improves the skin feel of gardenia essential oil, reduces greasy and heavy feeling, can also protect gardenia essential oil from being difficult to be oxidized and deteriorate, improves its efficacy stability, can also improve the permeability and bioavailability of gardenia essential oil, make it easier to be absorbed and utilized by the skin. Simultaneously, the gardenia essential oil nanoliposomes are compounded with lemon essential oil, rosemary essential oil, tea tree oil, beta-glucan, coenzyme Q10 and polyol, wherein beta-glucan can promote the percutaneous absorption of gardenia essential oil in coordination with liposomes, further promote the percutaneous absorption of other active components, and can also improve the moisturizing skin care effect of gardenia essential oil. Wherein lemon essential oil, rosemary essential oil, tea tree oil three-combination can further improve the skin feel of gardenia essential oil, can also promote each other in coordination with gardenia essential oil in the effects of moisturizing, anti-aging and delicate pores. Among them, coenzyme Q10 can protect the gardenia essential oil from being easily oxidized and deteriorated, improve its efficacy stability, and can also synergize with the gardenia essential oil to improve the anti-aging effect of the product. The above-mentioned functional components cooperate with each other, so that the final essential oil product has excellent moisturizing, anti-aging and pore-refining effects, as well as excellent skin feel and shelf stability. DETAILED DESCRIPTION

[0057] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0058] The sources of the effective ingredients contained in the products involved in the following preparation examples, embodiments and comparative examples are as follows (only the effective ingredients are reflected, and other necessary auxiliary ingredients contained in the commercially available raw materials are not repeated).

[0059] The gardenia essential oil mentioned below is a product purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory; lemon essential oil is a product purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory; rosemary essential oil is a product purchased from Hebei Qiansheng Biotechnology Co., Ltd.; tea tree oil is a product purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory; β-glucan is a product purchased from Heze High-tech Zone Youke Biotechnology Co., Ltd.; coenzyme Q10 is a product purchased from Suzhou Nakang Biotechnology Co., Ltd.; polyvinylpyrrolidone is a product purchased from Ashland LLC with model number pvp k-30powder.

[0060] Preparation Example 1

[0061] This preparation example provides a gardenia essential oil nanoliposome, and the preparation method is as follows:

[0062] (1) mixing soybean lecithin, cholesterol and gardenia essential oil in a mass ratio of 5:1:1 and dissolving them in anhydrous ethanol to form an oil phase liquid;

[0063] (2) removing anhydrous ethanol from the oil phase by rotary evaporation at 40°C, and drying at 60°C for 24 h under vacuum to form a dry lipid film;

[0064] (3) mixing the lipid film with phosphate buffer and polyvinyl pyrrolidone in a mass ratio of 5:10:1, and ultrasonically dispersing the mixture in a 60°C water bath to form an emulsion;

[0065] (4) The emulsion was centrifuged at 6000 rpm for 20 min to remove the upper essential oil and the lower impurities, and then filtered using a 0.22 μm microporous filter membrane to obtain the gardenia essential oil nanoliposomes.

[0066] Preparation Example 2

[0067] This preparation example provides a gardenia essential oil nanoliposome, and the preparation method is as follows:

[0068] (1) mixing and dissolving egg yolk lecithin, cholesterol and gardenia essential oil in anhydrous ethanol at a mass ratio of 4:2:1 to form an oil phase liquid;

[0069] (2) removing anhydrous ethanol from the oil phase by rotary evaporation at 45°C, and drying at 50°C for 30 h under vacuum to form a dry lipid film;

[0070] (3) mixing the lipid film with phosphate buffer and sodium carboxymethyl cellulose in a mass ratio of 3:8:1, and ultrasonically dispersing the mixture in a 50°C water bath to form an emulsion;

[0071] (4) The emulsion was centrifuged at 5000 rpm for 25 min to remove the upper essential oil and the lower impurities, and then filtered using a 0.22 μm microporous filter membrane to obtain the gardenia essential oil nanoliposomes.

[0072] Preparation Example 3

[0073] This preparation example provides a gardenia essential oil nanoliposome, and the preparation method is as follows:

[0074] (1) mixing soybean lecithin, cholesterol and gardenia essential oil in a mass ratio of 7:1:1 and dissolving them in anhydrous ethanol to form an oil phase liquid;

[0075] (2) removing anhydrous ethanol from the oil phase by rotary evaporation at 40°C, and drying at 65°C for 12 h under vacuum to form a dry lipid film;

[0076] (3) mixing the lipid film with phosphate buffer and polyvinyl pyrrolidone in a mass ratio of 5:15:1, and ultrasonically dispersing the mixture in a 65°C water bath to form an emulsion;

[0077] (4) The emulsion was centrifuged at 7000 rpm for 15 min to remove the upper essential oil and the lower impurities, and then filtered using a 0.22 μm microporous filter membrane to obtain the gardenia essential oil nanoliposomes.

[0078] Example 1

[0079] The present embodiment provides a skin care essential oil, and its preparation raw materials include, by mass, 30 parts of gardenia essential oil nanoliposomes prepared in Preparation Example 1, 8 parts of lemon essential oil, 12 parts of rosemary essential oil, 65 parts of tea tree oil, 6 parts of β-glucan, 1 part of coenzyme Q10, 5 parts of 1,2-hexanediol, and 5 parts of glycerol.

[0080] The preparation method is as follows:

[0081] (1) Heat tea tree oil to 50°C, then mix with lemon essential oil and rosemary essential oil and stir evenly;

[0082] (2) mixing with β-glucan, coenzyme Q10, 1,2-hexanediol and glycerol, stirring evenly, and cooling to 15°C;

[0083] (3) mixing evenly with gardenia essential oil liposome at 15° C. to obtain the skin care essential oil.

[0084] Example 2

[0085] The present embodiment provides a skin care essential oil, and its preparation raw materials include, by mass: 35 parts of gardenia essential oil nanoliposomes prepared in Preparation Example 2, 5 parts of lemon essential oil, 8 parts of rosemary essential oil, 60 parts of tea tree oil, 3 parts of β-glucan, 0.5 parts of coenzyme Q10, 10 parts of 1,2-hexanediol, and 5 parts of sorbitol.

[0086] The preparation method is as follows:

[0087] (1) Heat tea tree oil to 55°C, then mix with lemon essential oil and rosemary essential oil and stir evenly;

[0088] (2) Mixing and stirring with β-glucan, coenzyme Q10, 1,2-hexanediol and sorbitol, and cooling to 10°C;

[0089] (3) mixing evenly with gardenia essential oil liposome at 10° C. to obtain the skin care essential oil.

[0090] Example 3

[0091] The present embodiment provides a skin care essential oil, and its preparation raw materials include, by mass, 25 parts of gardenia essential oil nanoliposomes prepared in Preparation Example 3, 12 parts of lemon essential oil, 5 parts of rosemary essential oil, 70 parts of tea tree oil, 8 parts of β-glucan, 2 parts of coenzyme Q10, 5 parts of 1,3-propylene glycol, and 10 parts of glycerol.

[0092] The preparation method is as follows:

[0093] (1) Heat tea tree oil to 45°C, then mix with lemon essential oil and rosemary essential oil and stir evenly;

[0094] (2) Mixing with β-glucan, coenzyme Q10, 1,3-propylene glycol and glycerol, stirring evenly, and cooling to 15°C;

[0095] (3) mixing evenly with gardenia essential oil liposome at 15° C. to obtain the skin care essential oil.

[0096] Example 4

[0097] The present embodiment provides a skin care essential oil, and its preparation raw materials include, by mass, 30 parts of gardenia essential oil nanoliposomes prepared in Preparation Example 1, 8 parts of lemon essential oil, 12 parts of rosemary essential oil, 65 parts of tea tree oil, 6 parts of β-glucan, 1 part of coenzyme Q10, 5 parts of 1,2-hexanediol, 5 parts of glycerol, 9 parts of squalane, 0.1 parts of vitamin E, and 1 part of resveratrol.

[0098] The preparation method is as follows:

[0099] (1) Heat tea tree oil to 50°C, then mix with lemon essential oil and rosemary essential oil and stir evenly;

[0100] (2) mixing and stirring evenly with β-glucan, coenzyme Q10, 1,2-hexanediol, glycerol, squalane, vitamin E, and resveratrol, and cooling to 15° C.;

[0101] (3) mixing evenly with gardenia essential oil liposome at 15° C. to obtain the skin care essential oil.

[0102] Example 5

[0103] This embodiment provides a skin care essential oil, the raw materials for its preparation differ from those in embodiment 4 only in that resveratrol is missing, the mass fraction of squalane is 10 parts, and the other components and fractions remain unchanged. The preparation method is the same as that in embodiment 4.

[0104] Example 6

[0105] This embodiment provides a skin care essential oil, the raw materials for its preparation differ from those in Example 4 only in that vitamin E is missing, the mass fraction of coenzyme Q10 is 1.1 parts, and the other components and fractions remain unchanged. The preparation method is the same as in Example 4.

[0106] Comparative Example 1

[0107] This comparative example provides a skin care essential oil, the raw materials of which differ from those of Example 1 only in the lack of coenzyme Q10, the mass fraction of gardenia essential oil nanoliposomes is 31 parts, and the other components and fractions remain unchanged. The preparation method is the same as that of Example 1.

[0108] Comparative Example 2

[0109] This comparative example provides a skin care essential oil, the raw materials of which differ from those of Example 1 only in that β-glucan is missing, the mass fraction of gardenia essential oil nanoliposomes is 36 parts, and the other components and fractions remain unchanged. The preparation method is the same as that of Example 1.

[0110] Comparative Example 3

[0111] This comparative example provides a skin care essential oil, the raw materials of which differ from those in Example 1 only in that lemon essential oil is missing, and the reduced mass fraction thereof is proportionally distributed to gardenia essential oil nanoliposomes, rosemary essential oil and tea tree oil, while the other components and fractions remain unchanged. The preparation method is the same as in Example 1.

[0112] Comparative Example 4

[0113] This comparative example provides a skin care essential oil, the raw materials for its preparation differ from those in Example 1 only in that rosemary essential oil is missing, and the reduced mass fraction thereof is proportionally distributed to gardenia essential oil nanoliposomes, lemon essential oil and tea tree oil, while the other components and fractions remain unchanged. The preparation method is referred to Example 1.

[0114] Comparative Example 5

[0115] This comparative example provides a skin care essential oil, the raw materials of which differ from those in Example 1 only in that tea tree oil is missing, and the reduced mass fraction thereof is proportionally distributed to gardenia essential oil nanoliposomes, lemon essential oil and rosemary essential oil, while the other components and fractions remain unchanged. The preparation method is as in Example 1.

[0116] Comparative Example 6

[0117] This comparative example provides a skin care essential oil, the preparation raw materials of which differ from those of Example 1 only in that the gardenia essential oil nanoliposomes are missing, and the reduced mass fraction is proportionally distributed to tea tree oil, lemon essential oil and rosemary essential oil, while the other components and fractions remain unchanged. The preparation method is similar to that of Example 1.

[0118] Comparative Example 7

[0119] This comparative example provides a skin care essential oil, the preparation raw materials of which are different from those in Example 1 only in that 30 parts of gardenia essential oil nanoliposomes are replaced with 5 parts of gardenia essential oil, and the other components and parts remain unchanged. The preparation method is the same as in Example 1.

[0120] Test Example 1

[0121] Safety performance evaluation:

[0122] The safety performance of the products of Examples 1-6 and Comparative Examples 1-7 was evaluated. 40 social volunteers who met the requirements, aged 20-45 years old, were selected and randomly divided into 4 groups, and each group tried 3 or 4 different products. Placed in the small chamber of the spot tester, the control well was not treated in any way. The spot tester with the test substance was applied to the inner side of the forearm of the subject with a low-allergenic tape, and gently pressed with the palm of the hand to evenly apply it to the skin for 24 hours. Observe the skin reaction according to the standards in Table 1 at 30 minutes, 24 hours and 48 hours after removing the test substance spot tester.

[0123] Table 1

[0124]

[0125] The results show that the products of Examples 1-6 of the present invention and Comparative Examples 1-7 all had negative reactions after trial use, indicating that the skin care essential oils of the present invention are safe and mild, and will not cause adverse reactions such as skin irritation and sensitization.

[0126] Test Example 2

[0127] Moisturizing effect evaluation:

[0128] 130 subjects, aged between 28 and 40 years old with an average age of 32 years old, were selected and randomly divided into 13 groups. The subjects in each group were smeared with an equal amount of the products of Examples 1-6 of the present invention and Comparative Examples 1-7 on their faces. No products (cosmetics or external medicines) could be used on the test parts in the first 3 days.

[0129] Before the test, the subject cleans the face, air-dries it, and marks the measurement area. Before the test, sit quietly in a standard room for at least 30 minutes, do not drink water, and stay relaxed. Each person's facial mark is 2×2cm 2 Test area: Mark 3 areas with an interval of more than 1 cm between them, and test each group of samples in the test area separately.

[0130] The probe Corneometer CM825 was used to measure the skin moisture content of the test area and the control area. Each area was measured three times in parallel and the average value was taken. The blank value M0 of each test area was measured first, and then the subjects used the sample once in the morning and evening every day. The method and amount of use were kept consistent for 28 consecutive days. The skin moisture content M1 was tested on the 14th and 28th days. The test of the same subject was completed by the same measurement personnel. The increase in skin moisture content in each time period was calculated according to the following formula.

[0131]

[0132] The test results are shown in Table 2.

[0133] Table 2

[0134]

[0135]

[0136] It can be seen from the data results in Table 2 that the skin care essential oil involved in the present invention has excellent skin moisturizing effect. Comparative Example 1 and Comparative Example 7 show that loading gardenia essential oil in nanoliposomes can improve the permeability and bioavailability of gardenia essential oil, making it easier to be absorbed and utilized by the skin, thereby improving its moisturizing effect. Comparative Example 1 and Comparative Examples 3-6 show that gardenia essential oil nanoliposomes, lemon essential oil, rosemary essential oil, and tea tree oil have potential synergistic effects in moisturizing effect.

[0137] Test Example 3

[0138] Anti-aging effect evaluation:

[0139] The selection of subjects, preparation before testing and testing process are the same as those in Test Example 2. Before using the product and after 28 days of use, the skin elasticity meter Cutometer MPA580 was used to collect data. The test parameter was R2 (the ratio of the skin's rebound amount Ua without negative pressure to the maximum stretch amount Uf with negative pressure). The closer R2 is to 1, the better the skin's elasticity is. The average change rate of R2 is calculated. The average change rate of R2 = (R2 value on the 28th day - R2 value on the 0th day) / R2 value on the 0th day. The test results are shown in Table 3.

[0140] Test Example 4

[0141] Pore ​​refinement effect evaluation:

[0142] The selection of subjects, preparation before testing and testing process were the same as those in Test Example 2. After using the product for 28 days, VISIA was used to collect facial images and combined with Image-pro skin comprehensive analysis software for analysis, and the average skin pore area reduction rate of each group was statistically calculated. The statistical results are shown in Table 3.

[0143] Table 3

[0144]

[0145]

[0146] It can be seen from the data results in Table 3 that the skin care essential oil involved in the present invention has excellent skin firming and anti-aging effects. Comparative Example 1 and Comparative Example 7 show that loading gardenia essential oil in nanoliposomes can improve the permeability and bioavailability of gardenia essential oil, making it easier to be absorbed and utilized by the skin, thereby improving its anti-aging effect; Comparative Example 1 and Comparative Examples 3-6 show that gardenia essential oil nanoliposomes, lemon essential oil, rosemary essential oil, and tea tree oil have potential synergistic effects in firming and anti-aging effects; Comparative Example 1 and Comparative Example 1 show that coenzyme Q10 can synergize with gardenia essential oil to improve the anti-aging effect of the product; Comparative Example 1 and Examples 4-6 show that further adding squalane, vitamin E and resveratrol to the skin care essential oil formula can further improve its anti-aging effect.

[0147] The skin care essential oil involved in the present invention also has an excellent effect of refining pores. It can be seen from the data of Comparative Example 1 and Comparative Example 7, and from the data of Comparative Example 1 and Comparative Examples 3-6, it can be seen that gardenia essential oil nanoliposomes, lemon essential oil, rosemary essential oil, and tea tree oil have potential synergistic effects in the effect of refining pores.

[0148] Test Example 5

[0149] Human sensory evaluation:

[0150] The volunteers in test examples 2-4 evaluated the skin care essential oil products of Examples 1-6 and Comparative Examples 1-7 in terms of smearing, absorbency, stickiness, moisturizing, firmness, and pore improvement. The scoring criteria and grades are shown in Table 4 below:

[0151] Table 4

[0152]

[0153] The results are shown in Table 5 (all data are presented as average values):

[0154] Table 5

[0155] Group Smear feeling Absorbency Sticky feeling Moisturizing Compactness Pore ​​Improvement Example 1 4.6 4.5 4.5 4.4 4.2 4.0 Example 2 4.5 4.6 4.4 4.4 4.1 3.9 Example 3 4.4 4.3 4.3 4.5 4.2 3.9 Example 4 4.5 4.5 4.6 4.5 4.3 4.1 Example 5 4.3 4.3 4.3 4.2 4.2 4.0 Example 6 4.4 4.5 4.4 4.3 4.2 4.1 Comparative Example 1 4.5 4.5 4.4 4.2 4.0 4.1 Comparative Example 2 4.6 4.2 4.4 4.1 3.9 3.8 Comparative Example 3 4.2 4.5 4.2 4.3 4.0 3.9 Comparative Example 4 4.1 4.4 4.3 4.2 3.9 4.0 Comparative Example 5 3.6 3.8 3.5 3.8 3.6 3.8 Comparative Example 6 4.6 4.6 4.7 4.0 3.9 3.8 Comparative Example 7 2.0 2.8 1.6 3.7 3.5 3.3

[0156] It can be seen from the data results in Table 5 that the skin care essential oil involved in the present invention has excellent skin feel and skin care effect. Comparative Example 1 and Comparative Example 7 show that loading gardenia essential oil in nanoliposomes improves the skin feel of gardenia essential oil, reduces the greasy and heavy feeling, and is easier to apply and absorb; Comparative Example 1 and Comparative Examples 3-6 show that the combination of lemon essential oil, rosemary essential oil and tea tree oil can further enhance the skin feel of gardenia essential oil.

[0157] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0158] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0159] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A skin care essential oil with moisturizing, anti-aging and pore-refining effects, characterized in that: The components of the skin care essential oil include: gardenia essential oil nanoliposomes, lemon essential oil, rosemary essential oil, tea tree oil, beta-glucan, coenzyme Q10 and polyol.

2. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to claim 1, characterized in that: The components of the skin care essential oil include, by weight: 25-35 parts of gardenia essential oil nanoliposomes, 5-15 parts of lemon essential oil, 5-15 parts of rosemary essential oil, 60-80 parts of tea tree oil, 1-10 parts of beta-glucan, 0.1-5 parts of coenzyme Q10 and 5-30 parts of polyol.

3. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to claim 1 or 2, characterized in that: The gardenia essential oil nanoliposomes are prepared by a preparation method comprising the following steps: (1) mixing and dissolving phospholipids, cholesterol and gardenia essential oil in an organic solvent to form an oil phase liquid; (2) removing the organic solvent from the oil phase by rotary evaporation and drying to form a dry lipid film; (3) mixing the lipid film with a buffer and a surfactant, and ultrasonically dispersing the mixture to form an emulsion; (4) centrifuging the emulsion to remove the upper essential oil and the lower impurities, and filtering to obtain the gardenia essential oil nanoliposomes.

4. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to claim 3, characterized in that: The mass ratio of the phospholipid, cholesterol and gardenia essential oil is (3-8):(1-2):1; Preferably, the phospholipid comprises soybean lecithin or egg yolk lecithin; Preferably, the rotary evaporation is carried out at 35-50°C; Preferably, the drying is performed at 50-65° C. for 12-30 h under vacuum.

5. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to claim 3 or 4, characterized in that: The mass ratio of the lipid film, the buffer and the surfactant is (1-10):(3-20):1; Preferably, the buffer comprises phosphate buffer; Preferably, the surfactant comprises polyvinyl pyrrolidone or sodium carboxymethyl cellulose; Preferably, the ultrasonic dispersion is carried out in a water bath at 50-65°C; Preferably, the centrifugation is performed at 5000-7000 rpm for 10-60 min; Preferably, the filtration is performed using a 0.22 μm microporous filter membrane.

6. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to any one of claims 1 to 5, characterized in that: The polyol includes any one of ethylene glycol, propylene glycol, butylene glycol, pentanediol, hexanediol, glycerol, pentaerythritol or sorbitol, or a combination of at least two thereof.

7. The skin care essential oil with moisturizing, anti-aging and pore-refining effects according to any one of claims 1 to 6, characterized in that: The components of the skin care essential oil also include: any one or a combination of at least two of squalane, vitamin E or resveratrol; Preferably, the components of the skin care essential oil further include, by weight: any one of 2-16 parts of squalane, 0.02-0.2 parts of vitamin E or 1-1.5 parts of resveratrol, or a combination of at least two of them.

8. The method for preparing the skin care essential oil having moisturizing, anti-aging and pore-refining effects according to any one of claims 1 to 7, characterized in that: The preparation method comprises the following steps: (1) Heat tea tree oil and then mix with lemon essential oil and rosemary essential oil; (2) mixing with β-glucan, coenzyme Q10, and polyol and cooling; (3) mixing with gardenia essential oil liposomes to obtain the skin care essential oil.

9. The preparation method according to claim 8, characterized in that: The tea tree oil is heated to 40-55° C.; Preferably, step (2) further comprises mixing with any one or a combination of at least two of squalane, vitamin E or resveratrol; Preferably, the step (2) is cooled to 5-15°C; Preferably, the mixing in step (3) is followed by filtering.

10. Use of the skin care essential oil according to any one of claims 1 to 7 in preparing a product with moisturizing, anti-aging and pore-refining effects; Preferably, the product includes essential oil products, lotions, emulsions, essences, face creams, facial masks, make-up removal products or skin care products.

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