Tumor cell detection kit and detection method thereof

Abstract

The present invention belongs to the field of medical detection technology, especially a tumor cell detection kit and its detection method. Due to its single functionality, when the sample needs to be dispersed evenly during detection, if there is a lack of auxiliary equipment, the staff needs to operate manually, and the use effect is not good. The following scheme is now proposed, including a test kit body, an inner box frame is provided inside the test kit body, a circular hole is provided on the top of the inner box frame, and a detection uniformity module is provided inside the circular hole, and a fixed frame is fixedly connected to the top inner wall of the inner box frame. A tumor cell detection kit and its detection method disclosed by the present invention have the effect of improving the convenience of using the detection kit, and the device increases the use diversity of the test kit body, thereby facilitating the detection and use of the device, and reduces the auxiliary equipment required to be equipped when the device is used, further increasing the use effect of the device.

Description

Technical Field

[0001] The present invention relates to the field of medical detection technology, and in particular to a tumor cell detection kit and a detection method thereof. Background Art

[0002] Because the biological characteristics of tumors have not yet been fully understood, in addition to traditional imaging and pathological methods, there is currently a lack of simple, rapid, and non-invasive methods in clinical practice to evaluate the prognosis of tumor surgery or chemoradiotherapy, as well as effective means to track patient recurrence and disease progression.

[0003] The essence of tumor cells is the tumor. Tumor tissue is composed of two parts: the substance and the stroma. The substance of the tumor is the tumor cell, which is the main component of the tumor and has tissue-origin specificity. It determines the biological characteristics of the tumor and the particularity of each tumor. Usually, the tissue origin of various tumors is identified based on the substantial morphology of the tumor, and the tumor is classified, named and histologically diagnosed. The benign and malignant nature of the tumor and the degree of malignancy of the tumor are determined based on its degree of differentiation, maturity and the size of its heterogeneity. Tumor cells have three significant basic characteristics: immortality, migration and loss of contact inhibition. In addition, tumor cells have many physiological, biochemical and morphological characteristics that are different from normal cells.

[0004] The existing tumor detection kits have a single functionality when used. When the sample needs to be dispersed evenly during detection, if there is a lack of auxiliary equipment, the staff needs to operate manually, which results in poor results. In addition, due to its single functionality, it needs to be equipped with a variety of auxiliary equipment when used, which is inconvenient to use. Summary of the invention

[0005] The present invention discloses a tumor cell detection kit and a detection method thereof, aiming to solve the technical problems that the existing tumor detection kit in the background technology has a single functionality when in use, and when it is used for detection, when it is necessary to disperse the sample evenly, if there is a lack of auxiliary equipment, the staff needs to perform manual operation, and the use effect is not good. In addition, due to its single functionality, it needs to be equipped with a variety of auxiliary equipment when in use, which is inconvenient to use.

[0006] A tumor cell detection kit proposed in the present invention includes a kit body, an inner box frame is arranged inside the kit body, a circular hole is opened on the top of the inner box frame, and a detection uniformity module is arranged inside the circular hole, a fixed frame is fixedly connected to the top inner wall of the inner box frame, a fixed hole is opened on the bottom of the fixed frame, and a reagent dripping module is arranged inside the fixed hole, a reagent taking module is arranged inside the inner box frame, and an arc-shaped opening and a circular opening are opened on the top of the inner box frame, guide grooves are opened on the outer walls of both sides of the kit body, and the inner walls of the two guide grooves are movably connected with the same kit cover, and four elastic plates are arranged on the inner wall of the kit body, and the outer walls of the four elastic plates are in contact with the outer wall of the inner box frame.

[0007] By providing a reagent box body, an inner box frame, a reagent box cover, a detection uniformity module, a fixed frame, a reagent dripping module, a reagent taking module, a guide groove, an elastic plate and a circular hole, the detection uniformity module can evenly disperse the sample inside the detection tube when in use, without the need for manual operation by the staff, thereby increasing the convenience of using the device and increasing the diversity of the device when in use, and meeting the detection and use requirements of the device; the reagent dripping module can adjust the position and height of the dropper body when in use to ensure that the reagent drips into the inside of the detection tube, thereby increasing the use effect of the device, and multiple droppers are provided at the same time to avoid reagent mixing affecting the detection effect, thereby meeting the detection requirements; the reagent taking module can facilitate the staff to take the reagent bottle when in use, and when placing it, the device can elastically fix the reagent bottle to ensure the fixing effect and avoid damage; the elastic plate can increase the protective effect of the reagent box body on the internal device when in use.

[0008] In a preferred embodiment, the detection uniformity module includes a movable ball seat, which is movably connected to the inner wall of the circular hole, and the inner wall of the movable ball seat is fixedly connected with three pressure spring rods, one end of the three pressure spring rods is fixedly connected with a fixed clamping plate, and the same detection pipe fitting is arranged between the three fixed clamping plates, and the bottom of the movable ball seat is fixedly connected with a fixed ring frame, and the inner wall of the fixed ring frame is fixedly connected with a magnetic frame, and the outer wall of the magnetic frame is provided with three adjustment openings, and the inside of the three adjustment openings is provided with the same magnetic ring frame; three electric telescopic rods are fixedly connected to the magnetic frame, and the output ends of the three electric telescopic rods are fixedly connected to the top of the magnetic ring frame, and the inner wall of the magnetic ring frame is fixedly connected with a magnetic ring, and the magnetic ring is located inside the magnetic frame and outside the detection pipe fitting, and the inner wall of the magnetic frame is fixedly connected with a mounting plate; the bottom inner wall of the inner box frame is fixedly connected There is a mounting bracket, which is fixedly connected to a driving motor, and the output shaft of the driving motor is connected to a driving gear through a coupling, and the bottom inner wall of the inner box frame is movably connected to a transmission gear, the transmission gear is meshed with the driving gear, and the top of the transmission gear is fixedly connected to a fixed bracket, and the inner walls on both sides of the fixed bracket are movably connected to the same screw rod member, and the outer wall of the screw rod member is movably connected to a fixing member; two limit rods are fixedly connected to the inner walls on both sides of the fixed bracket, and the outer walls of the two limit rods are movably connected to the limit members, and the outer walls on opposite sides of the two limit members are respectively fixedly connected to the outer walls on both sides of the fixing member, a connecting rod is movably connected to the fixing member, and one end of the connecting rod is movably connected to a mounting member, the top of the mounting member is fixedly connected to the bottom of the mounting plate, and the outer wall on one side of the fixed bracket is fixedly connected to a stepping motor, and the output shaft of the stepping motor is connected to one end of the screw rod member through a coupling.

[0009] By providing a detection uniformity module, the detection uniformity module can automatically and evenly disperse the blood sample and reagent inside the detection tube when in use, thereby increasing the convenience of using the device when in use, and when in use for detection, the detection uniformity module can adjust the angle of the detection tube as needed, so as to facilitate the operations of evenly dispersing and resuspending the samples, increasing the diversity of use of the device, and at the same time, when in use, the device can also adjust the position of the magnetic ring as needed to perform magnetic attraction, further increasing the diversity of use of the device, thereby facilitating the use of the device for detection, and reducing the auxiliary equipment required for the use of the device, thereby increasing the use effect of the device.

[0010] In a preferred embodiment, the reagent taking module includes a support frame, which is fixedly connected to the bottom inner wall of the inner box frame, and a power motor is fixedly connected to the support frame. The output shaft of the power motor is connected to gear 2 through a coupling, and a reagent disk is fixedly connected to the top of gear 2, the outer wall of the reagent disk is in contact with the inner wall of the circular mouth, and an outer wall on one side of the support frame is movably connected to a mounting shaft rod, one end of the mounting shaft rod is fixedly connected to gear 1, gear 1 is meshed with gear 2, and a lifting member is fixedly connected to the outer wall on one side of gear 1; a fixed port is provided on the top of the reagent disk, an air pump is arranged inside the fixed port, a plurality of connecting pipes are fixedly connected to the output end of the air pump, the output ends of the plurality of connecting pipes are fixedly connected to connecting pipes, and two output ends of the plurality of connecting pipes are fixedly connected to air bag rings, and a plurality of reagent ports are provided on the top of the reagent disk, and the plurality of air bag rings are respectively located inside the plurality of reagent ports, and the inner walls of the two air bag rings inside the same reagent port are both provided with the same reagent bottle.

[0011] By providing a reagent taking module, the reagent taking module can lift the reagent bottle when taking it, so as to facilitate the staff to take the reagent bottle, thereby increasing the convenience of using the device. At the same time, the reagent taking module can also fix the reagent bottle when in use, and when fixing it, the device uses an air bag ring to fix it, so that the device can elastically fix the reagent bottle, thereby avoiding damage to the reagent bottle due to rigid fixation when the device is shaken, thereby increasing the use effect of the device.

[0012] In a preferred embodiment, the reagent dripping module includes an electric rod, which is fixedly connected to the inner wall of the fixing port, and the output end of the electric rod is fixedly connected to a mounting seat, and a mounting port is opened on the mounting seat, and the inner walls on both sides of the mounting port are movably connected to two shaft rods, and the outer walls of the two shaft rods are respectively fixedly connected to a winding wheel and a guide wheel, and the outer wall of the mounting seat is fixedly connected to a universal motor, and the output shaft of the universal motor is connected to one end of one of the shaft rods through a coupling; the top of the mounting seat is movably connected to a rotating knob cover, and the bottom of the rotating knob cover is fixedly connected to a plurality of telescopic spring rods, and the bottom ends of the plurality of telescopic spring rods are all fixedly connected to a connecting plate, and the plurality of connecting plates are all provided with a dropper body, and the top inner wall of the mounting port is fixedly connected to a reset spring rod, and the bottom end of the reset spring rod is fixedly connected to a clamping piece, and the inner wall of the clamping piece contacts the outer wall of one of the connecting plates, and a connecting cable is provided on the winding wheel, one end of the connecting cable is fixedly connected to the bottom of the clamping piece, and the outer wall of the connecting cable contacts the inner wall of the guide wheel.

[0013] By providing a reagent dripping module, the reagent dripping module can adjust the position and height of the dropper body when in use, thereby ensuring that the reagent can be added to the inside of the detection tube when in use, avoiding shaking of the handheld dropper body when adding the reagent, resulting in reagent waste, thereby increasing the use effect of the device, and when dripping the reagent, a plurality of dropper bodies are provided on the device, so that when adding multiple reagents, they can be distinguished to avoid contamination of the reagents, so as to ensure the detection effect.

[0014] A tumor cell detection method, using a tumor cell detection kit as described above, comprises the following steps:

[0015] Step 1: During the test, open the cover of the test kit and place the test tube containing the patient's blood inside the uniform detection module;

[0016] Step 2: Add reagents. When reagents need to be added during testing, start the reagent taking module. The reagent taking module will rotate so that the lifting part will lift the reagent bottle for easy taking.

[0017] Step 3: After the reagent is taken, the reagent is sucked through the dropper body. After sucking, the dropper body is set on the reagent dripping module, and the position and height of the dropper body are adjusted through the reagent dripping module until the reagent addition is completed;

[0018] Step 4. After adding the reagent, start the detection uniformity module, and adjust the angle of the detection tube through the detection uniformity module to evenly disperse the internal samples and reagents. When magnetic attraction is required, start the electric telescopic rod and adjust the position of the magnetic ring for magnetic attraction. When the sample needs to be resuspended, adjust the detection tube to a vertical state to resuspend the sample until the sample processing is completed and the test is performed.

[0019] From the above, it can be seen that the tumor cell detection kit provided by the present invention has the function of improving the convenience of using the detection kit. The device increases the usage diversity of the kit body, thereby facilitating the use of the device for detection, and reduces the auxiliary equipment required when using the device, further increasing the use effect of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall structure of a tumor cell detection kit proposed by the present invention;

[0021] Figure 2 This is a schematic cross-sectional structure diagram of an inner box frame of a tumor cell detection kit proposed by the present invention;

[0022] Figure 3 This is a schematic diagram of the combined structure of a detection tube and a dropper body of a tumor cell detection kit proposed by the present invention;

[0023] Figure 4 This is a schematic diagram of the uniform detection module structure of a tumor cell detection kit proposed by the present invention;

[0024] Figure 5 For the present invention Figure 4 Schematic diagram of some structures;

[0025] Figure 6 A schematic cross-sectional view of a movable ball seat of a tumor cell detection kit proposed by the present invention;

[0026] Figure 7 This is a schematic diagram of the structure of a reagent taking module of a tumor cell detection kit proposed by the present invention;

[0027] Figure 8 This is a schematic cross-sectional view of a reagent tray of a tumor cell detection kit proposed by the present invention;

[0028] Fig. 9 This is a schematic diagram of the structure of a reagent dripping module of a tumor cell detection kit proposed by the present invention;

[0029] Fig.10 For the present invention Fig. 9 Schematic diagram of some structures.

[0030] In the figure: 1, reagent box body; 2, guide groove; 3, inner box frame; 4, reagent dripping module; 401, electric rod; 402, mounting seat; 403, universal motor; 404, rotary knob cover; 405, connecting plate; 406, mounting port; 407, telescopic spring rod; 408, reset spring rod; 409, clamping part; 410, winding wheel; 411, connecting cable; 412, guide wheel; 413, shaft rod; 5, reagent box cover; 6, fixed frame; 7, detection uniformity module; 701, mounting bracket; 702, driving gear; 703, driving motor; 704, fixed ring frame; 705, movable ball seat; 706, fixed bracket; 707, transmission gear; 708, stepping motor; 7 09. Screw rod; 710. Limit rod; 711. Magnetic frame; 712. Electric telescopic rod; 713. Magnetic ring frame; 714. Fixed splint; 715. Pressure spring rod; 716. Mounting piece; 717. Connecting rod; 718. Limiting piece; 719. Fixing piece; 720. Mounting plate; 721. Magnetic ring; 8. Elastic plate; 9. Reagent taking module; 901. Support frame; 902. Power motor; 903. Mounting shaft; 904. Gear one; 905. Lifting piece; 906. Reagent tray; 907. Reagent bottle; 908. Gear two; 909. Air bag ring; 910. Air pump; 911. Connecting pipe; 912. Connecting pipe; 10. Dropper body; 11. Detection pipe. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] The tumor cell detection kit disclosed in the present invention is mainly used in scenarios where its functionality is single and when the sample needs to be dispersed evenly during detection, if there is a lack of auxiliary equipment, the staff needs to operate manually, resulting in poor use effect.

[0033] Reference Figure 1-Figure 10 A tumor cell detection kit comprises a kit body 1, an inner box frame 3 is arranged inside the kit body 1, a circular hole is opened on the top of the inner box frame 3, and a detection uniformity module 7 is arranged inside the circular hole, a fixed frame 6 is fixedly connected to the inner wall of the top of the inner box frame 3, a fixed hole is opened on the bottom of the fixed frame 6, and a reagent dripping module 4 is arranged inside the fixed hole, a reagent taking module 9 is arranged inside the inner box frame 3, and an arc-shaped opening and a circular opening are opened on the top of the inner box frame 3, guide grooves 2 are opened on the outer walls of both sides of the kit body 1, and the inner walls of the two guide grooves 2 are slidably connected with the same kit cover 5, and four elastic plates 8 are arranged on the inner wall of the kit body 1, and the outer walls of the four elastic plates 8 are in contact with the outer wall of the inner box frame 3.

[0034] Specifically, when in use, the detection uniformity module 7 can evenly disperse the sample inside the detection tube 11 without the need for manual operation by the staff, which increases the convenience of using the device and increases the diversity of the use of the device to meet the detection and use requirements of the device; when in use, the reagent dripping module 4 can adjust the position and height of the dropper body 10 to ensure that the reagent drips into the detection tube 11, increasing the use effect of the device, and at the same time, multiple droppers are set to avoid reagent mixing affecting the detection effect and meet the detection requirements; when in use, the reagent taking module 9 can facilitate the staff to take the reagent bottle 907, and when placing it, the device can elastically fix the reagent bottle 907 to ensure the fixing effect and avoid damage.

[0035] Reference Figure 2-Figure 6In a preferred embodiment, the uniform detection module 7 includes a movable ball seat 705, which is rotatably connected to the inner wall of the circular hole, and the inner wall of the movable ball seat 705 is fixedly connected with three pressure spring rods 715, and one end of each of the three pressure spring rods 715 is fixedly connected with a fixed clamping plate 714, and the same detection tube 11 is arranged between the three fixed clamping plates 714, and the bottom of the movable ball seat 705 is fixedly connected with a fixed ring frame 704, and the inner wall of the fixed ring frame 704 is fixedly connected with a magnetic frame 711, and the outer wall of the magnetic frame 711 is provided with three The inner wall of the magnetic frame 713 is fixedly connected with a magnetic ring 721, and the magnetic ring 721 is located inside the magnetic frame 711. The magnetic ring 721 is located outside the detection tube 11, and the inner wall of the magnetic frame 711 is fixedly connected with a mounting plate 720; the bottom inner wall of the inner box frame 3 is fixedly connected with a mounting bracket 701, and the mounting plate 720 is fixedly connected with the inner wall of the inner box frame 3. A driving motor 703 is fixedly connected to the mounting bracket 701, and the output shaft of the driving motor 703 is connected to the driving gear 702 through a coupling, and the bottom inner wall of the inner box frame 3 is connected to a transmission gear 707 through a bearing, and the transmission gear 707 is meshed with the driving gear 702. The top of the transmission gear 707 is fixedly connected to a fixed bracket 706, and the inner walls on both sides of the fixed bracket 706 are connected to the same screw rod 709 through bearings, and the outer wall of the screw rod 709 is connected to a fixing member 719 through a threaded connection; the inner walls on both sides of the fixed bracket 706 are fixedly connected to two There are a limit rod 710, and the outer walls of the two limit rods 710 are slidably connected to the limit members 718. The outer walls on the opposite sides of the two limit members 718 are fixedly connected to the outer walls on both sides of the fixing member 719 respectively. The fixing member 719 is rotatably connected with a connecting rod 717, and one end of the connecting rod 717 is rotatably connected with a mounting member 716. The top of the mounting member 716 is fixedly connected to the bottom of the mounting plate 720, and the outer wall on one side of the fixed bracket 706 is fixedly connected to a stepper motor 708, and the output shaft of the stepper motor 708 is connected to one end of the screw member 709 through a coupling.

[0036] Specifically, after the reagent is added, the stepper motor 708 is started, and the stepper motor 708 drives the screw rod 709 to rotate, and then the screw rod 709 drives the fixing member 719 to move, so that the fixing member 719 pulls the connecting rod 717 and the mounting member 716, and then drives the mounting plate 720, the magnetic frame 711, the fixing ring frame 704 and the movable ball seat 705 to change their angles, and then the driving motor 703 is started, and the driving motor 703 can drive the driving gear 702 to rotate. Since the driving gear 702 is meshed with the transmission gear 707, Thus, the transmission gear 707 can be rotated by driving the motor 703, and then the mounting plate 720, the magnetic frame 711, the fixed ring frame 704 and the movable ball seat 705 can be driven to rotate, so that the detection tube 11 can be rotated, so that the detection sample is evenly dispersed. When magnetic attraction is required, the electric telescopic rod 712 is started, and the position of the magnetic ring 721 is adjusted to perform magnetic attraction. When the sample needs to be resuspended, the screw rod 709 is rotated so that the screw rod 709 drives the fixing member 719 to reset, so that the detection tube 11 is in a vertical state to resuspend the sample.

[0037] In a specific application scenario, the detection uniformity module 7 is suitable for the detection sample processing link, that is, the detection uniformity module 7 can automatically and evenly disperse the blood sample and reagent inside the detection tube 11 when in use, thereby increasing the convenience of using the device when in use, and when used for detection, the detection uniformity module 7 can adjust the angle of the detection tube 11 as needed, so as to facilitate the operations of uniform dispersion and resuspending the samples, increasing the diversity of the use of the device, and at the same time, when in use, the device can also adjust the position of the magnetic ring 721 as needed to perform magnetic attraction, further increasing the diversity of the use of the device, thereby facilitating the use of the device for detection, and reducing the auxiliary equipment required to be equipped when using the device, increasing the use effect of the device.

[0038] Reference Figure 2 , Figure 7 and Figure 8In a preferred embodiment, the reagent taking module 9 includes a support frame 901, the support frame 901 is fixedly connected to the bottom inner wall of the inner box frame 3, a power motor 902 is fixedly connected to the support frame 901, the output shaft of the power motor 902 is connected to a gear 2 908 through a coupling, the top of the gear 2 908 is fixedly connected to a reagent disk 906, the outer wall of the reagent disk 906 is in contact with the inner wall of the circular mouth, and the outer wall of one side of the support frame 901 is connected to a mounting shaft rod 903 through a bearing, one end of the mounting shaft rod 903 is fixedly connected to a gear 1 904, and the gear 1 904 is meshed with the gear 2 908. A lifting piece 905 is fixedly connected to the outer wall of one side of the gear 904; a fixed port is provided on the top of the reagent tray 906, an air pump 910 is arranged inside the fixed port, a plurality of connecting tubes 911 are fixedly connected to the output end of the air pump 910, the output ends of the plurality of connecting tubes 911 are all fixedly connected to connecting tubes 912, both output ends of the plurality of connecting tubes 912 are fixedly connected to air bag rings 909, and a plurality of reagent ports are provided on the top of the reagent tray 906, the plurality of air bag rings 909 are respectively located inside the plurality of reagent ports, and the same reagent bottle 907 is provided on the inner walls of the two air bag rings 909 inside the same reagent port.

[0039] Specifically, when in use, start the air pump 910, and the air pump 910 transports the gas to the inside of the connecting tube 912 through the connecting tube 911, and then transports the gas to the inside of the airbag ring 909 through the connecting tube 912, so as to fix the reagent bottle 907 through the airbag ring 909. When it is necessary to add reagents during detection, start the power motor 902, and drive the gear 2 908 to rotate through the power motor 902. Since the gear 2 908 is meshed with the gear 1 904, the power motor 902 drives the gear 1 904 and the gear 2 908 to rotate synchronously, so as to drive the reagent tray 906 and the lifting member 905 to rotate. When the reagent tray 906 drives the reagent bottle 907 to move to the lifting member 905, the lifting member 905 can contact the bottom of the reagent bottle 907 to lift the reagent bottle 907 for easy removal;

[0040] In a specific application scenario, the reagent taking module 9 is suitable for the reagent bottle 907 taking link, that is, the reagent taking module 9 can lift the reagent bottle 907 when taking it, so as to facilitate the staff to take the reagent bottle 907, so as to increase the convenience of using the device. At the same time, the reagent taking module 9 can also fix the reagent bottle 907 when in use, and when fixing it, the device uses an airbag ring 909 for fixing, so that the device can elastically fix the reagent bottle 907, thereby avoiding damage to the reagent bottle 907 due to rigid fixation when the device is shaken, so as to increase the use effect of the device.

[0041] Reference Figure 1 , Figure 2 , Figure 3, Fig. 9 and Fig.10 In a preferred embodiment, the reagent dripping module 4 includes an electric rod 401, which is fixedly connected to the inner wall of the fixed port, and the output end of the electric rod 401 is fixedly connected to a mounting seat 402, and a mounting port 406 is provided on the mounting seat 402. The inner walls on both sides of the mounting port 406 are connected to two shaft rods 413 through bearings, and the outer walls of the two shaft rods 413 are respectively fixedly connected to a winding wheel 410 and a guide wheel 412, and the outer wall of the mounting seat 402 is fixedly connected to a universal motor 403, and the output shaft of the universal motor 403 is connected to one end of one of the shaft rods 413 through a coupling; the top of the mounting seat 402 is rotatably connected to A rotating knob cover 404 is provided with a plurality of telescopic spring rods 407 fixedly connected to the bottom of the rotating knob cover 404, a connecting plate 405 is fixedly connected to the bottom ends of the plurality of telescopic spring rods 407, a dropper body 10 is provided on the plurality of connecting plate members 405, and a return spring rod 408 is fixedly connected to the top inner wall of the mounting port 406, a clamping member 409 is fixedly connected to the bottom end of the return spring rod 408, an inner wall of the clamping member 409 is in contact with the outer wall of one of the connecting plate members 405, a connecting cable 411 is provided on the winding wheel 410, one end of the connecting cable 411 is fixedly connected to the bottom of the clamping member 409, and an outer wall of the connecting cable 411 is in contact with the inner wall of the guide wheel 412.

[0042] Specifically, after the reagent is taken, the reagent is absorbed through the dropper body 10. After absorbing, the dropper body 10 is set on the connecting plate 405, and the rotating knob cover 404 is rotated so that the rotating knob cover 404 drives the telescopic spring rod 407 and the connecting plate 405 to move to adjust the position of the dropper body 10. When the dropper body 10 moves to a suitable position, the clamping member 409 will be clamped with the connecting rod, and the universal motor 403 is started at this time. The universal motor 403 can drive the winding wheel 410 to reel the connecting cable 411, so that the connecting cable 411 pulls the clamping member 409 down. At this time, the reset spring rod 408 and the telescopic spring rod 407 are stretched until the dropper body 10 is adjusted to a suitable height for reagent addition. After adding, the universal motor 403 is reversed, the connecting cable 411 is relaxed, and the reset spring and the telescopic spring are restored to drive the clamping member 409 and the connecting plate 405 to reset, and the reagent addition is completed;

[0043] In a specific application scenario, the reagent dripping module 4 is suitable for the reagent adding link, that is, the reagent dripping module 4 can adjust the position and height of the dropper body 10 when in use, so as to ensure that the reagent can be added to the inside of the detection tube 11 when in use, and avoid shaking of the handheld dropper body 10 when adding the reagent, resulting in reagent waste, thereby increasing the use effect of the device, and when dripping the reagent, a plurality of dropper bodies 10 are arranged on the device, so as to distinguish them when adding multiple reagents, avoid contamination of the reagents, and ensure the detection effect.

[0044] A tumor cell detection method, using a tumor cell detection kit as described above, comprises the following steps:

[0045] Step 1: During the test, the reagent kit cover 5 is opened, and the test tube 11 containing the patient's blood is placed between the fixed clamps 714, and the fixed clamps 714 are driven by the pressure spring to clamp and fix the test tube 11;

[0046] Step 2, adding reagents. When reagents need to be added during testing, the power motor 902 is started, and the power motor 902 drives the gear 2 908 to rotate. Since the gear 2 908 is meshed with the gear 1 904, the power motor 902 drives the gear 1 904 and the gear 2 908 to rotate synchronously, so as to drive the reagent tray 906 and the lifting member 905 to rotate. When the reagent tray 906 drives the reagent bottle 907 to move to the lifting member 905, the lifting member 905 can contact the bottom of the reagent bottle 907 to lift the reagent bottle 907 for easy removal;

[0047] Step 3, after the reagent is taken, the reagent is absorbed through the dropper body 10. After absorbing, the dropper body 10 is set on the connecting plate 405, and the rotating knob cover 404 is turned, so that the rotating knob cover 404 drives the telescopic spring rod 407 and the connecting plate 405 to move to adjust the position of the dropper body 10. When the dropper body 10 moves to a suitable position, the clamping member 409 will be clamped with the connecting rod, and the universal motor 403 is started at this time. The universal motor 403 can drive the winding wheel 410 to reel the connecting cable 411, so that the connecting cable 411 pulls the clamping member 409 down, and the reset spring rod 408 and the telescopic spring rod 407 are stretched until the dropper body 10 is adjusted to a suitable height for reagent addition. After adding, the universal motor 403 is reversed, the connecting cable 411 is relaxed, and the reset spring and the telescopic spring are restored to drive the clamping member 409 and the connecting plate 405 to reset, and the reagent addition is completed;

[0048] Step 4: After the reagent is added, the stepper motor 708 is started, and the stepper motor 708 drives the screw rod 709 to rotate, and then the screw rod 709 drives the fixing member 719 to move, so that the fixing member 719 pulls the connecting rod 717 and the mounting member 716, and then drives the mounting plate 720, the magnetic frame 711, the fixing ring frame 704 and the movable ball seat 705 to change their angles, and then the driving motor 703 is started, and the driving motor 703 can drive the driving gear 702 to rotate. Since the driving gear 702 is meshed with the transmission gear 707, the driving gear 702 can be driven to rotate. The driving motor 703 drives the transmission gear 707 to rotate, which can drive the mounting plate 720, the magnetic frame 711, the fixed ring frame 704 and the movable ball seat 705 to rotate, so that the detection tube 11 rotates, so that the detection sample is evenly dispersed. When magnetic attraction is required, the electric telescopic rod 712 is started, and the position of the magnetic ring 721 is adjusted for magnetic attraction. When the sample needs to be resuspended, the screw rod 709 is rotated so that the screw rod 709 drives the fixing part 719 to reset, so that the detection tube 11 is in a vertical state to resuspend the sample until the sample processing is completed and the detection is carried out.

[0049] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A tumor cell detection kit, comprising a kit body (1), characterized in that: An inner box frame (3) is arranged inside the reagent box body (1), a circular hole is provided at the top of the inner box frame (3), and a uniform detection module (7) is arranged inside the circular hole; a fixed frame (6) is fixedly connected to the inner wall of the top of the inner box frame (3), a fixed hole is provided at the bottom of the fixed frame (6), and a reagent dripping module (4) is arranged inside the fixed hole; a reagent taking module (9) is arranged inside the inner box frame (3), and an arc-shaped opening and a circular opening are provided at the top of the inner box frame (3); guide grooves (2) are provided on the outer walls of both sides of the reagent box body (1), and the inner walls of the two guide grooves (2) are movably connected to the same reagent box cover (5); four elastic plates (8) are provided on the inner wall of the reagent box body (1), and the outer walls of the four elastic plates (8) are in contact with the outer wall of the inner box frame (3); The uniformity detection module (7) comprises a movable ball seat (705), the movable ball seat (705) being movably connected to the inner wall of the circular hole, three pressure spring rods (715) being fixedly connected to the inner wall of the movable ball seat (705), one end of each of the three pressure spring rods (715) being fixedly connected to a fixed clamping plate (714), a same detection pipe fitting (11) being arranged between the three fixed clamping plates (714), and a fixed ring frame (704) being fixedly connected to the bottom of the movable ball seat (705), a magnetic frame (711) being fixedly connected to the inner wall of the fixed ring frame (704), and three adjustment openings being provided on the outer wall of the magnetic frame (711), and a same magnetic ring frame (713) being arranged inside the three adjustment openings.

2. A tumor cell detection kit according to claim 1, characterized in that: The magnetic frame (711) is fixedly connected to three electric telescopic rods (712); the output ends of the three electric telescopic rods (712) are fixedly connected to the top of the magnetic ring frame (713); the inner wall of the magnetic ring frame (713) is fixedly connected to a magnetic ring (721); the magnetic ring (721) is located inside the magnetic frame (711); the magnetic ring (721) is located outside the detection tube (11); and the inner wall of the magnetic frame (711) is fixedly connected to a mounting plate (720).

3. A tumor cell detection kit according to claim 2, characterized in that: The bottom inner wall of the inner box frame (3) is fixedly connected to a mounting bracket (701), the mounting bracket (701) is fixedly connected to a driving motor (703), the output shaft of the driving motor (703) is connected to a driving gear (702) via a coupling, and the bottom inner wall of the inner box frame (3) is movably connected to a transmission gear (707), the transmission gear (707) is meshed with the driving gear (702), the top of the transmission gear (707) is fixedly connected to a fixing bracket (706), the inner walls on both sides of the fixing bracket (706) are movably connected to the same screw rod (709), and the outer wall of the screw rod (709) is movably connected to a fixing member (719).

4. A tumor cell detection kit according to claim 3, characterized in that: Two limiting rods (710) are fixedly connected to the inner walls on both sides of the fixed bracket (706); the outer walls of the two limiting rods (710) are movably connected to limiting members (718); the outer walls on opposite sides of the two limiting members (718) are respectively fixedly connected to the outer walls on both sides of the fixed member (719); the fixed member (719) is movably connected to a connecting rod (717); one end of the connecting rod (717) is movably connected to a mounting member (716); the top of the mounting member (716) is fixedly connected to the bottom of the mounting plate (720); and a stepping motor (708) is fixedly connected to the outer wall on one side of the fixed bracket (706); the output shaft of the stepping motor (708) is connected to one end of the screw member (709) via a coupling.

5. A tumor cell detection kit according to claim 4, characterized in that: The reagent taking module (9) comprises a support frame (901), the support frame (901) is fixedly connected to the bottom inner wall of the inner box frame (3), a power motor (902) is fixedly connected to the support frame (901), the output shaft of the power motor (902) is connected to gear 2 (908) via a coupling, the top of gear 2 (908) is fixedly connected to a reagent tray (906), the outer wall of the reagent tray (906) is in contact with the inner wall of the circular mouth, and an outer wall on one side of the support frame (901) is movably connected to a mounting shaft rod (903), one end of the mounting shaft rod (903) is fixedly connected to gear 1 (904), gear 1 (904) is meshed with gear 2 (908), and a lifting member (905) is fixedly connected to an outer wall on one side of the gear 1 (904).

6. A tumor cell detection kit according to claim 5, characterized in that: The reagent tray (906) is provided with a fixed opening at the top, an air pump (910) is arranged inside the fixed opening, the output end of the air pump (910) is fixedly connected to a plurality of connecting tubes (911), the output ends of the plurality of connecting tubes (911) are all fixedly connected to connecting tubes (912), the two output ends of the plurality of connecting tubes (912) are all fixedly connected to air bag rings (909), and a plurality of reagent ports are provided at the top of the reagent tray (906), the plurality of air bag rings (909) are respectively located inside the plurality of reagent ports, and the inner walls of the two air bag rings (909) located inside the same reagent port are both provided with the same reagent bottle (907).

7. A tumor cell detection kit according to claim 6, characterized in that: The reagent dripping module (4) comprises an electric rod (401), the electric rod (401) is fixedly connected to the inner wall of the fixed opening, the output end of the electric rod (401) is fixedly connected to a mounting seat (402), a mounting opening (406) is provided on the mounting seat (402), two shaft rods (413) are movably connected to the inner walls on both sides of the mounting opening (406), the outer walls of the two shaft rods (413) are respectively fixedly connected to a winding wheel (410) and a guide wheel (412), and the outer wall of the mounting seat (402) is fixedly connected to a universal motor (403), and the output shaft of the universal motor (403) is connected to one end of one of the shaft rods (413) through a coupling.

8. A tumor cell detection kit according to claim 7, characterized in that: The top of the mounting seat (402) is movably connected to a rotating knob cover (404), the bottom of the rotating knob cover (404) is fixedly connected to a plurality of telescopic spring rods (407), the bottom ends of the plurality of telescopic spring rods (407) are fixedly connected to a connecting plate (405), the plurality of connecting plates (405) are provided with a dropper body (10), the top inner wall of the mounting opening (406) is fixedly connected to a return spring rod (408), the bottom end of the return spring rod (408) is fixedly connected to a clamping member (409), the inner wall of the clamping member (409) is in contact with the outer wall of one of the connecting plates (405), the winding wheel (410) is provided with a connecting cable (411), one end of the connecting cable (411) is fixedly connected to the bottom of the clamping member (409), and the outer wall of the connecting cable (411) is in contact with the inner wall of the guide wheel (412).

9. A method for detecting tumor cells, using a tumor cell detection kit as claimed in claim 8, characterized in that: The steps include: Step 1: During the test, the reagent kit cover (5) is opened, and the test tube (11) containing the patient's blood is placed inside the uniform detection module (7); Step 2: Add reagents. When reagents need to be added during testing, the reagent taking module (9) is started. The reagent taking module (9) rotates so that the lifting member (905) lifts up the reagent bottle (907) for easy taking. Step 3: After the reagent is taken, the reagent is sucked through the dropper body (10). After the reagent is sucked, the dropper body (10) is placed on the reagent dripping module (4), and the position and height of the dropper body (10) are adjusted through the reagent dripping module (4) until the reagent addition is completed; Step 4: After the reagent is added, the detection uniformity module (7) is started, and the angle of the detection tube (11) is adjusted through the detection uniformity module (7) to evenly disperse the internal sample and reagent. When magnetic attraction is required, the electric telescopic rod (712) is started, and the position of the magnetic ring (721) is adjusted to perform magnetic attraction. When the sample needs to be resuspended, the detection tube (11) is adjusted to be in a vertical state to resuspend the sample until the sample processing is completed and the detection is performed.

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