A kind of black wormwood rhizome crushing device and extraction method for extracting biological active substances from black wormwood

Abstract

The present invention belongs to the technical field of extracting biologically active substances from black wormwood, and in particular, to a black wormwood rhizome crushing device and extraction method for extracting biologically active substances from black wormwood, comprising a frame, a crushing box is installed on the top of the frame, a crushing chamber is opened on the crushing box, a powder hopper is provided below the crushing box, a hopper is fixedly installed through the top of the crushing box, a crushing mechanism is provided in the crushing chamber, and a driving mechanism is provided on the frame. The present invention cooperates with two-component cutting blades and two arc filter plates. After the two-component cutting blades crush and cut the black wormwood rhizomes, the two arc filter plates are used to screen the cut black wormwood rhizomes, and by sorting out large-particle black wormwood rhizomes, it is convenient for the subsequent two-component cutting blades to crush and cut them again, and the two grinding rollers can crush and grind the small-particle black wormwood rhizomes sorted by the arc filter plates.

Description

Technical Field

[0001] The invention relates to the technical field of extracting bioactive substances from Artemisia sphaerocephala, and in particular to a device for crushing Artemisia sphaerocephala rhizomes for extracting bioactive substances from Artemisia sphaerocephala and an extraction method. Background Art

[0002] Artemisia Ordosica Krasch., also known as Ordos Artemisia and Artemisia ordosica, is widely distributed in sand dunes and has good wind-proof and sand-fixing properties. It tastes bitter and is warm in nature. It has a long history of application in the medical field. Both its branches and leaves can be used as medicine. Artemisia Ordosica Krasch. is rich in biochemical components. Many studies have shown that the plant contains a wealth of various components, including flavonoids, terpenes, phenylpropanoids, polysaccharides, steroids, organic acids, anthraquinones, amino acids and trace elements. Among them, polyphenols and flavonoids have multiple pharmacological activities such as antibacterial, antiviral, antioxidant, anti-tumor, hypoglycemic, anti-inflammatory, analgesic, immunity-enhancing, anti-allergic, and anti-parasitic activities. They are currently the research hotspots of the active ingredients of Artemisia plants.

[0003] The common extraction method of active ingredients of Artemisia sphaerocephala is to use traditional organic solvents for extraction. These organic solvents include ethanol, acetone, dichloromethane, petroleum ether, ethyl acetate, n-butanol, etc. However, the organic solvent extraction method has the disadvantages of long cycle, which may take overnight or several days, and the extractant is easy to volatilize into the surrounding environment, resulting in safety hazards or pollution, and the extractant is difficult to recover, with large consumption, high cost, low extraction yield, etc., which is not conducive to large-scale industrial production and application. For example, Chinese patent application CN115844938A discloses a method of using a Soxhlet extractor with a concentration of 40-90% under the assistance of ultrasound. % ethanol solution to extract total flavonoids from Artemisia sphaerocephala; CN110960565A discloses a method for layered extraction of active substances from Artemisia sphaerocephala using traditional solvents (such as ethanol, petroleum ether, ethyl acetate and n-butanol) and its application in the treatment of allergic rhinitis. The extraction process of these two methods is time-consuming, the types of biologically active substances are not completely extracted, and it is impossible to simultaneously extract terpenes, polyphenols and flavonoids from Artemisia sphaerocephala. Therefore, it is urgent to provide a new, more environmentally friendly method that can extract multiple biologically active substances from Artemisia sphaerocephala and has a faster extraction speed to promote the industrial application of Artemisia sphaerocephala and the development of health products.

[0004] Deep Eutectic Solvents (DES) are a class of mixtures composed of hydrogen bond donors and hydrogen bond acceptors, which usually form a liquid state at a relatively low temperature. They have the advantages of being environmentally friendly, simple to prepare, and low in cost, and are increasingly valued in the extraction of natural products. CN115925602A provides a method for extracting lutein from marigold using a deep eutectic solvent, wherein marigold particles are soaked in an ethanol / ethyl acetate solution of a deep eutectic solvent, allowed to stand, filtered, and a filtrate is obtained; the filtrate is concentrated and then adsorbed by a macroporous adsorption resin, eluted with an eluent, the eluent is collected, and concentrated under reduced pressure to obtain a lutein ointment, wherein menthol / acetic acid, menthol / caproic acid, thymol / caproic acid, lauric acid / octanoic acid, choline chloride / acetic acid, L-carnitine / hexadecene Hydrophobic low eutectic solvents such as fluoroisopropanol, choline chloride / phenol, methyl trioctyl ammonium chloride / n-butanol, tetraheptyl ammonium chloride / ibuprofen, atropine / thymol, etc., and the low eutectic solvent uses a large amount of ethanol or ethyl acetate as a solvent. CN117126219A proposes a method for extracting astilbin from Smilax glabra using a natural low eutectic solvent, and the low eutectic solvent is a hydrophobic low eutectic solvent composed of choline chloride / lactic acid in a ratio of 1:1-2. These schemes all use hydrophobic low eutectic solvents when using natural low eutectic solvents, and do not combine hydrophilic low eutectic solvents and hydrophobic low eutectic solvents for the extraction of natural active ingredients in plants. At present, there is no related report on using biphasic DES as an extractant to simultaneously extract multiple active substances such as terpenes, polyphenols and flavonoids in natural plants.

[0005] Secondly, a crushing device is needed to make the rhizomes of black wormwood into powder, but because the rhizome fibers of black wormwood have better toughness, when traditional crushing equipment crushes the rhizomes of black wormwood, the rhizome fibers with better toughness are easily entangled with each other, thereby affecting the working effect of the crushing equipment. For this reason, a black wormwood rhizome crushing device and an extraction method for extracting bioactive substances from black wormwood are proposed. Summary of the invention

[0006] In order to solve the shortcomings of the prior art, the invention provides a rhizome crushing device and an extraction method for extracting biologically active substances from black wormwood.

[0007] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood, comprising a frame, a crushing box is installed on the top of the frame, a crushing chamber is opened on the crushing box, a powder hopper is provided under the crushing box, a hopper is fixedly installed through the top of the crushing box, a crushing mechanism is provided in the crushing chamber, and a driving mechanism is provided on the frame. After the black wormwood rhizomes are put into the hopper, they enter the crushing chamber, and then the driving mechanism drives the crushing mechanism to crush, cut and grind the black wormwood rhizomes, and the black wormwood rhizome powder produced by grinding will fall into the powder hopper.

[0008] Preferably, the crushing mechanism includes two rotating plates rotatably installed in the crushing chamber, two rotating columns and two prisms are rotatably installed on the two rotating plates, the two rotating columns and the two prisms are staggered and distributed in a circular array, the fixed sleeve on the rotating column is provided with a plurality of linearly distributed cutting blades, the sliding sleeve on the prism is provided with a grinding roller, two rotating disks are rotatably installed on the rotating plates, and the two prisms respectively penetrate the corresponding rotating disks and are fixedly connected to the corresponding rotating disks.

[0009] Preferably, a gear ring is provided on the side of the two rotating plates away from each other, and the sides of the two gear rings away from each other are respectively fixedly connected to the inner walls of the two sides of the crushing chamber, and gear 1 is fixedly installed at both ends of the rotating column, and the two gear 1s are respectively meshed with the corresponding gear rings, and gear 2 is fixedly installed at both ends of the prism, and the two gear 2s are respectively meshed with the corresponding gear rings, and the driving mechanism includes a rotating shaft that passes through the crushing box and is rotatably connected to the crushing box, the rotating shaft passes through the two rotating plates and is fixedly connected to the two rotating plates, a motor is fixedly installed on one side of the frame, and pulley 2 and pulley 1 are respectively fixedly installed on the end of the rotating shaft and the output shaft of the motor, and the same synchronous belt is provided on pulley 1 and pulley 2.

[0010] Preferably, a reciprocating mechanism is provided on both sides of the grinding roller, and the reciprocating mechanism includes two guide rods fixedly installed on the end of the grinding roller and two L-shaped rods fixedly installed on the side of the rotating plate away from the grinding roller, the two L-shaped rods have the same annular block fixedly installed on one end close to each other, the two guide rods pass through the rotating plate and are slidably connected to the rotating plate, and rollers are fixedly installed on the end of the two guide rods away from the grinding roller, and the two rollers are rollingly connected to the annular block.

[0011] Preferably, the annular block is provided with two inner recesses and two outer protrusions on one side close to the rotating disk, the two inner recesses and the two outer protrusions are staggered and connected end to end, and the roller is adapted to the two inner recesses and the two outer protrusions.

[0012] Preferably, two arc filter plates are fixedly installed between the two rotating plates, the ends of the two arc filter plates are slidably connected to the inner wall of the crushing chamber, and the two grinding rollers are respectively located on the inner sides of the corresponding arc filter plates, a feed port is provided at the top of the crushing chamber, the hopper is fixedly connected to the inner wall of the feed port, a discharge port is provided at the bottom of the crushing chamber, a feed discharge filter plate is fixedly installed in the discharge port, a discharge port is provided on the frame, the bottom of the crushing box is fixedly connected to the top of the frame, the discharge port and the discharge port are communicated, and a feed discharge pipe connected to the discharge port is fixedly installed at the bottom of the frame.

[0013] A method for efficiently extracting bioactive substances from Artemisia sphaerocephala root powder, comprising extracting Artemisia sphaerocephala root powder with a biphasic low eutectic solvent, wherein the biphasic low eutectic solvent consists of a hydrophilic low eutectic solvent and a hydrophobic low eutectic solvent, wherein the hydrogen bond acceptor in the hydrophilic low eutectic solvent is betaine, and the hydrogen bond donor is ethylene glycol; the hydrogen bond acceptor in the hydrophobic low eutectic solvent is menthol, and the hydrogen bond donor is isopropanol; and the volume ratio of the hydrophilic low eutectic solvent to the hydrophobic low eutectic solvent is 3-1:1-3.

[0014] According to a preferred embodiment of the present invention, the combined molar ratio of betaine and ethylene glycol in the hydrophilic deep eutectic solvent is 1:1-6. The abbreviation of the hydrophilic deep eutectic solvent is betaine-ethylene glycol or Bet-EG.

[0015] According to a preferred embodiment of the present invention, the combined molar ratio of menthol and isopropanol in the hydrophobic deep eutectic solvent is 1:1-6. The abbreviation of the hydrophobic deep eutectic solvent is menthol-isopropanol, abbreviated as Men-Iso. Preferably, the molar ratio of menthol and isopropanol in the hydrophobic DES is 1:2.

[0016] According to a preferred embodiment of the present invention, the preparation method of the hydrophilic low eutectic solvent is: betaine, ethylene glycol and water are mixed to obtain a mixture, wherein the water content of the mixture is 7-15wt%; the mixture is heated at 40°C-90°C and stirred for 10-30 minutes to form a uniform and transparent solution, and the mixture is cooled to room temperature to obtain a hydrophilic low eutectic solvent (abbreviated as NADES).

[0017] According to a preferred embodiment of the present invention, the water content of the hydrophilic low eutectic solvent is 7-15wt%, and most preferably 7wt%. As the water content increases, the viscosity of NADES (hydrophilic low eutectic solvent) decreases, which helps the mass transfer in the extraction process, but when the water content exceeds the optimal value, the increase in water may weaken or even destroy the hydrogen bonding effect inside NADES, causing the extraction efficiency to gradually decrease. Preferably, the combined molar ratio of betaine and ethylene glycol is 1:1-6. When the water content of the hydrophilic low eutectic solvent is 7wt.%, the extraction rates of polyphenols and ketones both reach the maximum, and increasing or decreasing the water content shows a downward trend.

[0018] When the water content is fixed at 7wt.%, since a uniform solution cannot be formed when the Bet-EG molar ratio is in the range of 3-1:1, a uniform solution can be obtained by adjusting the betaine:ethylene glycol molar ratio to 1:1-6. Moreover, as the molar ratio changes between 1:1-6, the overall changes in total polyphenols TPC and total ketones TFC are not very obvious, indicating that the effect of ethylene glycol content on the Bet-EG extraction ability is not obvious.

[0019] According to a preferred embodiment of the present invention, the preparation method of the hydrophobic low eutectic solvent is as follows: menthol and isopropanol are mixed to obtain a mixture, the mixture is heated at 40°C-90°C and stirred for 20-60 minutes to form a uniform and transparent solution, and the mixture is cooled to room temperature to obtain a hydrophobic low eutectic solvent (abbreviated as HDES). The hydrophobic low eutectic solvent does not require additional heating of solvents such as water, ethanol or acetate during the preparation process.

[0020] The hydrophilic deep eutectic solvent and the hydrophobic deep eutectic solvent prepared by the above method are cooled in a desiccator containing silica gel and stored for later use.

[0021] According to a preferred embodiment of the present invention, the method for extracting biologically active substances from Artemisia sphaerocephala is:

[0022] S1, mixing a hydrophilic deep eutectic solvent and a hydrophobic deep eutectic solvent to form a two-phase deep eutectic solvent;

[0023] S2, after the rhizome of Artemisia sphaerocephala is made into powder, it is added to the two-phase deep eutectic solvent, and after ultrasonic extraction, centrifugal slag removal, filtration and impurity removal, etc., an extract containing biologically active substances of Artemisia sphaerocephala root is obtained; wherein, 50-200mL of the two-phase deep eutectic solvent is added to every 1g of Artemisia sphaerocephala root powder. Preferably, 50-150mL of the two-phase deep eutectic solvent is added to every 1g of Artemisia sphaerocephala root powder.

[0024] According to a preferred embodiment of the present invention, the ultrasonic time is 10-120 min, the power is 200-500 W, and the temperature during the extraction process is controlled at 30-70° C. Preferably, the ultrasonic time is 120 min, the power is 400-500 W, and the temperature is 40° C. Under this condition, the total extraction rate of active ingredients in Artemisia sphaerocephala is the highest.

[0025] The experiment found that the extraction equilibrium state was reached after 120 minutes of ultrasound, and the effect of further increasing the application of ultrasound on the extraction rate was not obvious. When the ultrasonic power increased between 200-500W, the extraction rate increased slightly, and the ultrasonic power of 400-500W had a higher extraction rate for active substances. When the extraction temperature was 40°C, the total extraction rate of active substances was the highest. Temperatures below or above this temperature resulted in a significant decrease in the extraction rate of active substances.

[0026] According to a preferred embodiment of the present invention, the centrifugation time is 10-30 min, and the centrifugation speed is 3000-10000 rpm.

[0027] According to a preferred embodiment of the present invention, the biologically active substances of Artemisia sphaerocephala include terpenes, polyphenols and flavonoids; the extract is concentrated by vacuum rotary evaporation and freeze-dried to obtain the biologically active substances of Artemisia sphaerocephala.

[0028] According to a preferred embodiment of the present invention, the biologically active substances of Artemisia sphaerocephala include oleanolic acid and echinocystic acid.

[0029] Oleanolic acid and Echinocystic acid are both naturally occurring pentacyclic triterpenoid compounds, which have good application prospects in anti-inflammatory, free radical scavenging, antibacterial, antiviral, anti-tumor, liver protection, hypoglycemic, immunomodulatory, promoting wound healing, and improving skin conditions.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The present invention cooperates with two-component cutting blades and two arc-shaped filter plates. After the two-component cutting blades crush and cut the rhizomes of Artemisia sphaerocephala, the two arc-shaped filter plates are used to screen the crushed rhizomes of Artemisia sphaerocephala. Large-grained rhizomes of Artemisia sphaerocephala are sorted out, so that the two-component cutting blades can crush and cut them again in the future. The two grinding rollers can crush and grind the small-grained rhizomes of Artemisia sphaerocephala separated by the arc-shaped filter plates.

[0032] 2. The two grinding rollers can not only rotate around the rotating shaft to complete the crushing of the rhizomes of black wormwood, but also can rotate on their own during the revolution around the rotating shaft. In the process of self-rotation, the grinding rollers can also reciprocate along the axis as a path under the cooperation of the two reciprocating mechanisms, thereby completing the grinding of the rhizomes of black wormwood;

[0033] 3. Traditional single-phase hydrophilic DES or hydrophobic DES, water, ethanol, methanol, ethanol-water mixed extractants can only extract part of the active substances in black wormwood, and cannot simultaneously and efficiently extract terpenes, polyphenols and flavonoids, especially oleanolic acid and echinocystic acid. The present invention provides a method for extracting biologically active substances such as terpenes, polyphenols and flavonoids from black wormwood using a biphasic DES. The biphasic DES is composed of a hydrophilic DES betaine-ethylene glycol and a hydrophobic DES menthol-isopropanol in a molar ratio of 1-3:3-1. Experiments have shown that the biphasic DES can effectively improve the total extraction efficiency of biologically active substances such as terpenes (such as oleanolic acid and echinocystic acid), polyphenols and flavonoids in the roots of black wormwood, which is of great significance for improving the opening of commercial products of biologically active substances such as terpenes, polyphenols and flavonoids in the roots of black wormwood. Compared with organic solvents, low eutectic solvents are more environmentally friendly.

[0034] 4. In the extraction process, ultrasonic assistance is preferably used. Ultrasonic assistance does not affect the chemical structure and activity of the product, and can significantly improve the extraction rate of polyphenols and flavonoid compounds, which is suitable for small-scale trial production. The hydrogen bond acceptor of the hydrophilic deep eutectic solvent used in the present invention is betaine, and the hydrogen bond acceptor of the hydrophobic deep eutectic solvent is menthol, both of which are natural substances with good environmental protection. In addition, due to the short extraction time, the deep eutectic solvent is not easy to escape into the environment and can be efficiently recycled, which has the advantages of being green and environmentally friendly.

[0035] 5. The method of the present invention has the advantages of simple process flow, short time consumption, easy operation, low extraction temperature, low energy consumption, and active ingredients are not easy to denature. It solves the problems of low efficiency of traditional solvents, long extraction cycle, environmental pollution, high temperature destruction of material structure, and inability to extract multiple active substances simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 The overall structure of the Artemisia sphaerocephala rhizome crushing device for extracting biologically active substances from Artemisia sphaerocephala proposed by the present invention is shown in FIG. Figure 1 ;

[0037] Figure 2 The overall structure of the Artemisia sphaerocephala rhizome crushing device for extracting biologically active substances from Artemisia sphaerocephala proposed by the present invention is shown in FIG. Figure 2 ;

[0038] Figure 3 A side cross-sectional view of a crushing box in a device for crushing rhizomes of Artemisia sphaerocephala for extracting biologically active substances from Artemisia sphaerocephala proposed by the present invention, as well as a schematic structural diagram of a crushing mechanism and two reciprocating mechanisms;

[0039] Figure 4 This is a schematic structural diagram of a rotating shaft, a crushing mechanism and a reciprocating mechanism in a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood proposed by the present invention;

[0040] Figure 5 for Figure 4 A schematic diagram of the enlarged structure of part A;

[0041] Figure 6 It is a side cross-sectional view of a crushing box and a crushing mechanism in a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood proposed by the present invention;

[0042] Figure 7 It is a structural schematic diagram of two reciprocating mechanisms in a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood proposed by the present invention and a partial schematic diagram of the crushing mechanism;

[0043] Figure 8 for Figure 7 A schematic diagram of the enlarged structure of part B;

[0044] Fig. 9 This is a schematic structural diagram of a frame and a powder hopper in a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood proposed by the present invention;

[0045] Fig.10 The contents of oleanolic acid and echinocystic acid in the extracts of Examples 1-13 and Comparative Examples 1-18 are compared.

[0046] Fig.11 The figure is a comparison of the content of each active ingredient in the extracts of Examples 1-13 and Comparative Examples 19-30.

[0047] Fig.12 The content of each active ingredient in the extract of Example 1 is compared with that of Comparative Examples 31-41.

[0048] In the figure: 1. frame; 11. discharge port; 12. feed pipe; 2. crushing box; 21. crushing chamber; 22. discharge port; 23. feed filter plate; 24. feed port; 3. hopper; 4. driving mechanism; 41. rotating shaft; 42. motor; 43. pulley 1; 44. pulley 2; 5. crushing mechanism; 51. rotating plate; 52. rotating column; 53. gear 1; 54. cutting blade; 55. gear ring; 56. prism; 57. gear 2; 58. grinding roller; 59. rotating disk; 510. arc filter plate; 6. reciprocating mechanism; 61. guide rod; 62. roller; 63. annular block; 631. inner concave portion; 632. outer convex portion; 64. L-shaped rod; 7. powder hopper. DETAILED DESCRIPTION

[0049] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0050] Please refer to Figure 1-Figure 12 The present invention provides a technical solution: a black wormwood rhizome crushing device for extracting biologically active substances from black wormwood, comprising a frame 1, a crushing box 2 is installed on the top of the frame 1, a crushing chamber 21 is opened on the crushing box 2, a powder hopper 7 is provided below the crushing box 2, a hopper 3 is fixedly installed on the top of the crushing box 2, a crushing mechanism 5 is provided in the crushing chamber 21, a driving mechanism 4 is provided on the frame 1, and the black wormwood rhizome is put into the hopper 3 and then enters the crushing chamber 21, and then the driving mechanism 4 drives the crushing mechanism 5 to crush, cut and grind the black wormwood rhizome, and the black wormwood rhizome powder produced by grinding will fall into the powder hopper 7.

[0051] The crushing mechanism 5 includes two rotating plates 51 rotatably installed in the crushing chamber 21, two rotating columns 52 and two prisms 56 are rotatably installed on the two rotating plates 51, the two rotating columns 52 and the two prisms 56 are staggered and distributed in a circular array, a plurality of linearly distributed cutting blades 54 are fixedly sleeved on the rotating columns 52, a grinding roller 58 is slidably sleeved on the prisms 56, two rotating disks 59 are rotatably installed on the rotating plates 51, and the two prisms 56 respectively penetrate the corresponding rotating disks 59 and are fixedly connected to the corresponding rotating disks 59.

[0052] A gear ring 55 is provided on the side of the two rotating plates 51 away from each other, and the sides of the two gear rings 55 away from each other are respectively fixedly connected to the inner walls of the crushing chamber 21 on both sides, and a gear 1 53 is fixedly installed on both ends of the rotating column 52, and the two gear 1s 53 are respectively meshed and connected with the corresponding gear rings 55, and a gear 2 57 is fixedly installed on both ends of the prism 56, and the two gear 2s 57 are respectively meshed and connected with the corresponding gear rings 55. The driving mechanism 4 includes a rotating shaft 41 that penetrates the crushing box 2 and is rotatably connected to the crushing box 2, the rotating shaft 41 penetrates the two rotating plates 51 and is fixedly connected to the two rotating plates 51, and a motor 42 is fixedly installed on one side of the frame 1, and a pulley 2 44 and a pulley 1 43 are respectively fixedly installed on the end of the rotating shaft 41 and the output shaft of the motor 42, and the same synchronous belt is sleeved on the pulley 1 43 and the pulley 2 44.

[0053] Furthermore, when the two rotating plates 51 drive the two rotating columns 52 and the two prisms 56 to rotate around the rotating shaft 41 as the center, with the cooperation of gear 1 53 and the gear ring 55 and the cooperation of gear 2 57 and the gear ring 55, the rotating column 52 and the prism 56 will also rotate. The rotating rotating column 52 will drive the cutting blade 54 to rotate. The rotation of the cutting blade 54 can enable its circular cutting edge to alternately crush and cut the rhizomes of black wormwood, thereby avoiding the situation where a part of the cutting edge is continuously crushed and cut the rhizomes of black wormwood and caused damage.

[0054] A reciprocating mechanism 6 is provided on both sides of the grinding roller 58, and the reciprocating mechanism 6 includes two guide rods 61 fixedly installed at the end of the grinding roller 58 and two L-shaped rods 64 fixedly installed on the side of the rotating plate 51 away from the grinding roller 58. The ends of the two L-shaped rods 64 close to each other are fixedly installed with the same annular block 63, and the two guide rods 61 both penetrate the rotating disk 59 and are slidably connected to the rotating disk 59. The ends of the two guide rods 61 away from the grinding roller 58 are fixedly installed with rollers 62, and the two rollers 62 are rollingly connected to the annular block 63.

[0055] The side of the annular block 63 close to the rotating disk 59 is provided with two inner recesses 631 and two outer protrusions 632 , which are staggered and connected end to end, and the roller 62 is adapted to the two inner recesses 631 and the two outer protrusions 632 .

[0056] Furthermore, the annular blocks 63 at both ends of the grinding roller 58 are arranged in the same direction, and the guide rods 61 at both ends of the grinding roller 58 are distributed in a vertical state, so when the two outer protrusions 632 at one end of the grinding roller 58 contact with the corresponding inner concave parts 631, the two outer protrusions 632 at the other end will contact with the corresponding outer protrusions 632;

[0057] When the prism 56 drives the grinding roller 58 to rotate, the two guide rods 61 at the end of the grinding roller 58 will drive the two rollers 62 to move between the staggered inner concave parts 631 and the outer convex parts 632, so that the grinding roller 58 will reciprocate along its own axis while rotating. Therefore, the grinding roller 58 will reciprocate along its own axis while crushing the rhizomes of black wormwood to grind the rhizomes of black wormwood.

[0058] Two arc filter plates 510 are fixedly installed between the two rotating plates 51, and the ends of the two arc filter plates 510 are slidably connected to the inner wall of the crushing chamber 21, and the two grinding rollers 58 are respectively located on the inner sides of the corresponding arc filter plates 510. A feed port 24 is provided at the top of the crushing chamber 21, and the hopper 3 is fixedly connected to the inner wall of the feed port 24. A discharge port 22 is provided at the bottom of the crushing chamber 21, and a discharge filter plate 23 is fixedly installed in the discharge port 22. A discharge port 11 is provided on the frame 1, and the bottom of the crushing box 2 is fixedly connected to the top of the frame 1, and the discharge port 22 is connected to the discharge port 11, and a discharge pipe 12 connected to the discharge port 11 is fixedly installed at the bottom of the frame 1.

[0059] In this embodiment: first, the two grinding rollers 58 are placed in a horizontal state, and then the chopped black wormwood rhizomes are put into the hopper 3, and then the black wormwood rhizomes fall into the crushing chamber 21 and are located between the two arc-shaped filter plates 510, and then the motor 42 is started to drive the pulley 43 to rotate, and the pulley 43 then drives the rotating shaft 41 to rotate through the synchronous belt, and the rotating shaft 41 then drives the entire crushing mechanism 5 to rotate with the axis of the rotating shaft 41 as the center through the two rotating plates 51;

[0060] like Figure 6 As shown, when the two rotating columns 52 rotate clockwise around the rotating shaft 41, the rotating column 52 drives the multiple cutting blades 54 to cooperate with the unloading filter plate 23, so that the chopped black wormwood rhizomes can be crushed and cut again. After that, when the arc filter plate 510 scrapes over the crushed and cut black wormwood rhizomes, large particles of black wormwood rhizomes will fall back onto the unloading filter plate 23 along the outer side of the arc filter plate 510, which is convenient for the multiple cutting blades 54 on the other rotating column 52 to crush and cut them again, and small particles of black wormwood rhizomes will enter the arc filter plate 510, and then the small particles of black wormwood rhizomes will fall on the top of the unloading filter plate 23 again, and then the grinding roller 58 on the inner side of the arc filter plate 510 can crush and grind the small particles of black wormwood rhizomes that fell on the top of the unloading filter plate 23.

[0061] The powder produced by grinding will pass through the discharge filter plate 23 and fall into the discharge pipe 12 and finally fall into the powder hopper 7. The rhizomes of Artemisia sphaerocephala that have not been ground into powder will be pushed over the grinding roller 58, and then the rhizomes of Artemisia sphaerocephala that have not been ground into powder will pass through the arc filter plate 510 and fall back on the top of the discharge filter plate 23, so as to facilitate the next rolling, cutting and rolling grinding.

[0062] The invention provides a method for extracting bioactive substances such as terpenes, polyphenols and flavonoids from black wormwood by using a dual-phase DES. The dual-phase DES is composed of a hydrophilic DES betaine-ethylene glycol and a hydrophobic DES menthol-isopropanol according to a molar ratio of 1-3:3-1. The dual-phase DES can fully extract bioactive substances such as terpenes, polyphenols and flavonoids from black wormwood, and avoids the problem that traditional solvents such as water, methanol, ethanol, ethanol-water, single-phase hydrophilic DES or single-phase hydrophobic DES cannot fully and synchronously extract bioactive substances such as terpenes, polyphenols and flavonoids from black wormwood.

[0063] In the process of preparing the hydrophilic low eutectic solvent, betaine, ethylene glycol and water are used to prepare it, and in the process of preparing the hydrophobic low eutectic solvent, menthol and isopropanol are used to prepare it, without adding water or other solvents.

[0064] In the extraction process of the present invention, ultrasonic assisted extraction is preferably applied. Ultrasonic extraction has the following advantages: (1) High extraction efficiency: The mechanical action of ultrasound can reduce the viscosity of the low eutectic solvent and promote the dispersion of natural products in the two-phase DES; the small bubbles formed by cavitation will continue to move, grow or suddenly burst with the vibration of the surrounding medium, causing the surrounding liquid to suddenly rush into the bubbles to generate high temperature and high pressure, and at the same time generate shock waves, effectively improving the mass transfer between liquid and solid; promoting the wall breaking or deformation of plant cell tissue, accelerating the penetration of solvent into plant cells, making the effective components of natural products more fully extracted, and the extraction rate is significantly improved by 50-500% compared with the traditional process; (2) Fast extraction speed: Ultrasonic wave strengthens the extraction of natural products. The best extraction rate can usually be achieved within 10-120 minutes, and the extraction speed is significantly improved compared with traditional methods. (3) Low extraction temperature: The optimal temperature for ultrasonic extraction of natural products is 20-60°C, which has a protective effect on the active ingredients in medicinal materials that are unstable when exposed to heat and easily hydrolyzed or oxidized. This temperature is also the temperature at which ultrasound exerts its best effect. (4) Wide adaptability: Ultrasonic extraction of natural products is not limited by the polarity or molecular weight of the ingredients and is suitable for the extraction of most types of natural products and various ingredients. (5) The extract has few impurities and the active ingredients are easy to separate and purify. (6) The extraction process has low energy consumption cost, is simple and easy to operate, and is easy to maintain and maintain, with significant overall economic benefits.

[0065] The scheme and effects of the present invention are described below in conjunction with specific embodiments and comparative examples.

[0066] Embodiment 1:

[0067] This embodiment provides a method for rapidly extracting bioactive substances such as terpenes (such as oleanolic acid and echinocystic acid), polyphenols and flavonoids from the roots of Artemisia sphaerocephala, comprising the following steps:

[0068] (1) Preparation of hydrophilic DES and hydrophobic DES to form a biphasic DES

[0069] Preparation of hydrophilic DES: betaine, ethylene glycol and water are mixed so that the molar ratio of betaine to ethylene glycol is 1:2 to obtain a mixture with a water content of 7 wt.%; the mixture is heated and stirred at 70°C for 30 minutes to form a uniform and transparent solution, which is then cooled to room temperature to obtain a hydrophilic low eutectic solvent (abbreviated as NADES).

[0070] Preparation of hydrophobic DES: Menthol and isopropanol were mixed in a molar ratio of 1:3, and the mixture was heated and stirred at 60°C for 30 minutes to form a uniform and transparent solution, which was then cooled to room temperature to obtain a hydrophobic low eutectic solvent (abbreviated as HDES).

[0071] The above-mentioned NADES and HDES are combined in a volume ratio of 1:1 to obtain a biphasic DES.

[0072] (2) Take 1 g of dried Artemisia sphaerocephala root powder, add the prepared biphasic DES at a solid-liquid ratio of 1:100 (g / mL), place it in an ultrasonic-assisted device, perform ultrasonic extraction for 120 min, the ultrasonic power is 500 w, the ultrasonic temperature is 40 °C, and centrifuge at 5000 rpm for 5 min to remove the residue to obtain an extract.

[0073] (3) The total polyphenol content in the lower hydrophilic DES (NADES) was determined by the Folin phenol method. The total flavonoid content was determined by the NaNO2-Al(NO3)3-NaOH colorimetric method. The oleanolic acid and echinocystic acid contents were determined by HPLC.

[0074] Among them: the determination of total polyphenol (TPC) content uses gallic acid as the standard, and a standard curve of different concentrations is drawn; the final total polyphenol content is expressed in mg gallic acid equivalent / g black wormwood (mg GAE / g); the determination of total flavonoids (TFC) content uses rutin as the standard, and a standard curve of different concentrations is drawn, and the final total flavonoids content is expressed in mg rutin equivalent / g black wormwood (mgRE / g).

[0075] Among them, the detection method of active ingredients of oleanolic acid and echinocystic acid in the extract is:

[0076] The active ingredients of each extract were detected using Dalian Yiteli EClassical3100 high performance liquid chromatograph (HPLC) (5μmC18 column (4.6×250mm)), with a constant column temperature of 30°C, a detection wavelength of 215nm, and a constant flow rate of 1mL / min for the mobile phase. The injection volume was 20μL, the mobile phase A was acetonitrile, and B was a 0.1wt.% formic acid aqueous solution. The elution was performed at a volume ratio of 60:40 between phase A and phase B. The standards and samples were filtered with a 0.22μm nylon filter membrane to remove impurities before injection. Before the next injection, the column was rinsed with the initial proportion of mobile phase for 10-20min to balance the column.

[0077] Embodiment 2:

[0078] The only difference between this embodiment and embodiment 1 is that the molar ratio of menthol to isopropyl alcohol in the hydrophobic DES is adjusted to 1:2. The preparation method and conditions of NADES are the same as those in embodiment 1.

[0079] Embodiment 3:

[0080] The only difference between this embodiment and embodiment 1 is that the molar ratio of menthol to isopropyl alcohol in the hydrophobic DES is adjusted to 1:4. The preparation method and conditions of NADES are the same as those in embodiment 1.

[0081] Embodiment 4:

[0082] The only difference between this embodiment and embodiment 1 is that the molar ratio of menthol to isopropyl alcohol in the hydrophobic DES is adjusted to 1:6. The preparation method and conditions of NADES are the same as those in embodiment 1.

[0083] Embodiment 5:

[0084] The only difference between this embodiment and embodiment 1 is that the molar ratio of betaine to ethylene glycol in the hydrophilic DES is adjusted to 1:1. The preparation method and conditions of HDES are the same as those in embodiment 1.

[0085] Embodiment 6:

[0086] The only difference between this embodiment and embodiment 1 is that the molar ratio of betaine to ethylene glycol in the hydrophilic DES is adjusted to 1:3. The preparation method and conditions of HDES are the same as those in embodiment 1.

[0087] Embodiment 7:

[0088] The only difference between this embodiment and embodiment 1 is that the molar ratio of betaine to ethylene glycol in the hydrophilic DES is adjusted to 1:4. The preparation method and conditions of HDES are the same as those in embodiment 1.

[0089] Embodiment 8:

[0090] The only difference between this embodiment and embodiment 1 is that the molar ratio of betaine to ethylene glycol in the hydrophilic DES is adjusted to 1:6. The preparation method and conditions of HDES are the same as those in embodiment 1.

[0091] Embodiment 9:

[0092] The difference between this embodiment and embodiment 1 is that the hydrophilic DES and the hydrophobic DES are combined in a volume ratio of 1:2. The other conditions are the same as those in embodiment 1.

[0093] Embodiment 10:

[0094] The difference between this embodiment and embodiment 1 is that the hydrophilic DES and the hydrophobic DES are combined in a volume ratio of 2:1. The other conditions are the same as those in embodiment 1.

[0095] Embodiment 11:

[0096] The difference between this embodiment and embodiment 1 is that the hydrophilic DES and the hydrophobic DES are combined in a volume ratio of 1:3. The other conditions are the same as those in embodiment 1.

[0097] Embodiment 12:

[0098] The difference between this embodiment and embodiment 1 is that the hydrophilic DES and the hydrophobic DES are combined in a volume ratio of 3:1. The other conditions are the same as those in embodiment 1.

[0099] Embodiment 13:

[0100] The difference between this embodiment and embodiment 1 is that the water content of the hydrophilic DES is 15% during the preparation process. The other conditions are the same as those in embodiment 1.

[0101] Comparative Examples 1-18:

[0102] Comparative Examples 1-18 used conventional extractants or single-phase hydrophilic DES (NADES) as extractants as shown in the following table (Table 1), and used the same extraction method as Example 1 for extraction, and detected oleanolic acid and echinocystic acid in the extract according to the method of Example 1. Unless otherwise specified, the molar ratio of hydrogen bond acceptors to hydrogen bond donors in NADES was 1:1.

[0103] Table 1: Traditional extractants and 14 NADES extractants

[0104]

[0105] The contents of oleanolic acid and echinocystic acid in the extracts of Examples 1-13 and Comparative Examples 1-18 are as follows: Fig.10 See also Fig.10As shown in the figure, the extracts extracted by various single-phase NADES did not detect the content of oleanolic acid and echinocystic acid by HPLC. The content of oleanolic acid and echinocystic acid was also not detected in the extracts extracted by traditional extractants (water, methanol, ethanol-water mixture).

[0106] Comparative Examples 19-30:

[0107] Comparative Examples 19-30 used single-phase hydrophobic DES (HDES) as the extraction agent as shown in the following table (Table 2), and the same extraction method as Example 1 was used for extraction, and the oleanolic acid and echinocystic acid, total flavonoids (TFC), and polyphenols (TPC) in the extract were detected according to the method of Example 1.

[0108] Table 2: 12 HDES extractants

[0109] Group Abbreviation name Comparative Example 19 Men-Iso Menthol / Isopropyl Alcohol Comparative Example 20 Men-NBA Menthol / n-Butyl Alcohol Comparative Example 21 Men-NCA Menthol / octanol Comparative Example 22 Men-LA Menthol / Levulinic Acid Comparative Example 23 Men-GA-Iso Menthol / Glycolic Acid / Isopropyl Alcohol Comparative Example 24 Men-PEG200 Menthol / Polyethylene Glycol 200 Comparative Example 25 Men-PEG600-Iso Menthol / Polyethylene Glycol 600 / Isopropyl Alcohol Comparative Example 26 Men-Gly-Iso Menthol / Glycerin / Isopropyl Alcohol Comparative Example 27 Men-1,2PG Menthol / 1,2-Propylene Glycol Comparative Example 28 Men-1,4But Menthol / 1,4-Butylene Glycol Comparative Example 29 Men-1,3PG Menthol / 1,3-Propylene Glycol Comparative Example 30 Men-EG Menthol / Glycol

[0110] Among them, when HDES has only one hydrogen bond donor, the molar ratio of menthol to the hydrogen bond donor is 1:2, and when there are two hydrogen bond donors, the molar ratio of menthol to the hydrogen bond donor is 1:2:1 according to the naming order.

[0111] The contents of each active ingredient in the extracts of Examples 1-13 and Comparative Examples 19-30 are shown in Table 3.

[0112] Table 3: Extraction effect of HDES of Examples 1-13 and Comparative Examples 19-30 on Artemisia sphaerocephala

[0113] Group Oleanolic acid (mg / g) Echinocystic acid (mg / g) Total flavonoids (mg / g) Total polyphenols (mg / g) Total ingredients (mg / g) Example 1 3.835 0.528 16.24 7.58 28.187 Example 2 2.418 0.670 30.57 17.35 51.013 Example 3 2.339 0.720 15.44 7.38 25.879 Example 4 2.331 0.740 14.62 7.27 24.961 Example 5 2.512 0.690 18.78 15.56 37.542 Example 6 2.667 0.633 16.27 13.41 32.98 Example 7 2.411 0.700 15.12 7.21 25.441 Example 8 2.387 0.740 13.12 6.98 23.227 Example 9 3.941 0.514 14.10 6.88 25.435 Example 10 2.876 0.655 28.91 16.57 49.011 Embodiment 11 3.944 0.522 13.12 6.38 23.966 Example 12 2.310 0.790 25.613 16.081 44.794 Example 13 2.120 0.499 16.04 7.28 25.939 Comparative Example 19 1.807 0.277 / 1.868 3.952 Comparative Example 20 1.811 0.282 / 1.789 3.882 Comparative Example 21 1.832 0.279 / 1.912 4.023 Comparative Example 22 2.664 0.227 4.072 5.000 11.519 Comparative Example 23 2.817 0.219 5.110 4.589 12.735 Comparative Example 24 3.112 0.301 2.717 5.112 11.242 Comparative Example 25 3.052 0.286 2.284 5.063 10.685 Comparative Example 26 1.668 0.254 15.289 0.452 17.663 Comparative Example 27 1.640 0.270 5.179 0.411 7.500 Comparative Example 28 1.720 0.261 12.549 0.418 17.948 Comparative Example 29 1.630 0.246 8.172 0.407 10.455 Comparative Example 30 1.724 0.235 7.053 1.768 10.780

[0114] Note: “ / ” in the table means that the solvent interferes with the determination of the component, and the accurate content value cannot be measured under the same conditions.

[0115] From the above results, it can be seen that the extracts obtained by various single-phase hydrophobic DES did not detect flavonoids by the NaNO2-Al(NO3)3-NaOH colorimetric method. In addition, from the extraction effect of oleanolic acid and echinocystic acid, the content of oleanolic acid and echinocystic acid was not detected in the extracts obtained by traditional solvents and single-phase hydrophilic DES, and the content of oleanolic acid and echinocystic acid in the extracts obtained by single-phase hydrophobic DES was also very low, and the extraction effect was poor. Only the biphasic DES composed of hydrophilic DES and hydrophobic DES can maximize the extraction of oleanolic acid and echinocystic acid as well as flavonoids and polyphenols in black wormwood. This shows that in the biphasic DES, the hydrophilic DES can promote the extraction ability of HDES for terpenes (oleanolic acid and echinocystic acid), and the hydrophilic DES and hydrophobic DES in the biphasic DES have a synergistic effect in extracting active substances of black wormwood. From the comparison between Example 1 and Example 13, it can be seen that the extraction effect of the dual-phase DES combined when the water content in the hydrophilic DES is 7 wt.% on Artemisia sphaerocephala is better than that when the water content is 15 wt.%.

[0116] Comparative Examples 31-41:

[0117] Comparative Examples 31-41 used biphasic DES as an extractant and used the same extraction method as Example 1 for extraction, and detected oleanolic acid and echinocystic acid in the extract according to the method of Example 1. The hydrophilic DES in the biphasic DES was the same as that in Example 1, and was prepared by adding 7% betaine and ethylene glycol in a molar ratio of 1:2, wherein the hydrophobic DES was as shown in the following table (Table 4).

[0118] Table 4: Hydrophobic DES in biphasic DES (hydrophilic DES refer to Example 1)

[0119] Group Abbreviation name Example 1 Men-Iso Menthol / Isopropyl Alcohol Comparative Example 31 Men-NBA Menthol / n-Butyl Alcohol Comparative Example 32 Men-NCA Menthol / octanol Comparative Example 33 Men-LA Menthol / Levulinic Acid Comparative Example 34 Men-GA-Iso Menthol / Glycolic Acid / Isopropyl Alcohol Comparative Example 35 Men-PEG200 Menthol / Polyethylene Glycol 200 Comparative Example 36 Men-PEG600-Iso Menthol / Polyethylene Glycol 600 / Isopropyl Alcohol Comparative Example 37 Men-Gly-Iso Menthol / Glycerin / Isopropyl Alcohol Comparative Example 38 Men-1,2PG Menthol / 1,2-Propylene Glycol Comparative Example 39 Men-1,4But Menthol / 1,4-Butylene Glycol Comparative Example 40 Men-1,3PG Menthol / 1,3-Propylene Glycol Comparative Example 41 Men-EG Menthol / Glycol

[0120] The contents of the active ingredients in the extracts of Example 1 and Comparative Examples 31-41 are as follows: Fig.12 As shown. Fig.12 It can be seen that the biphasic DES can indeed simultaneously extract polyphenols, flavonoids and terpenoids such as oleanolic acid and echinocystic acid from Artemisia sphaerocephala. However, in terms of extraction effect, the biphasic DES composed of menthol-isopropyl alcohol and betaine-ethylene glycol in Example 1 has the best extraction effect on Artemisia sphaerocephala, and the total amount of flavonoids, polyphenols and terpenoids obtained is the largest.

[0121] Embodiment 14-17:

[0122] In Examples 14-17, the extraction temperature in Example 1 was changed to 30°C, 50°C, 60°C and 70°C, respectively, and the other conditions were the same as those in Example 1. Oleanolic acid, echinocystic acid and other active substances in the extract were extracted according to the method of Example 1. The composition of the biphasic DES was the same as that in Example 1.

[0123] Table 5: Content of each active ingredient in the extracts of Example 1 and Examples 14-17

[0124] Group Oleanolic acid (mg / g) Echinocystic acid (mg / g) Total flavonoids (mg / g) Total polyphenols (mg / g) Total ingredients (mg / g) Example 1 3.835 0.528 16.24 7.584 28.187 Embodiment 14 3.774 0.517 16.026 7.489 27.806 Embodiment 15 2.779 0.65 15.23 7.02 25.679 Example 16 2.667 0.66 15.1 7.188 25.615 Embodiment 17 3.512 0.489 14.21 5.78 23.991

[0125] From the above experimental results, it can be seen that when the extraction temperature is 40℃, the biphasic DES has the best extraction effect on the active ingredients extracted from Artemisia sphaerocephala, followed by 30℃ and 50℃.

[0126] Comparative Examples 42-45:

[0127] The main difference between Comparative Examples 42-45 and Examples 1 and 13 is that the water content in the hydrophilic DES is changed to 0, 30 wt.%, 45 wt.%, and 60 wt.%, respectively, and then the hydrophobic DES is combined to form a biphasic DES. The hydrophobic DES is the same as that in Example 1. Oleanolic acid, echinocystic acid and other active substances in the extract are extracted according to the method of Example 1.

[0128] Table 6: Table of active ingredient contents in the extracts of Examples 1, 13 and Comparative Examples 42-45

[0129] Group Oleanolic acid (mg / g) Echinocystic acid (mg / g) Total flavonoids (mg / g) Total polyphenols (mg / g) Total ingredients (mg / g) Example 1 3.835 0.528 16.24 7.584 28.187 Example 13 2.12 0.499 16.04 7.28 25.939 Comparative Example 42 1.35 0.312 14.21 4.28 20.152 Comparative Example 43 1.23 0.298 12.12 3.88 17.528 Comparative Example 44 1.19 0.278 11.29 3.67 16.428 Comparative Example 45 1.11 0.223 10.12 3.58 15.033

[0130] From the comparison of the above extraction results, it can be seen that when preparing biphasic DES, the water content in the hydrophilic DES should be controlled at 7-15wt% for better extraction effect, especially when the water content is controlled at 7wt%, the combined biphasic DES has the best total extraction effect on the active substances of Artemisia sphaerocephala.

[0131] In addition, when the extraction time is constant, the ultrasonic power changes between 200-500W, which has little effect on the extraction effect of Artemisia sphaerocephala. The total amount of active substances increases slowly as the power increases. The total amount of active substances extracted reaches the maximum value at 500W, which is 1.15 times the total amount of 400W ultrasonic extraction and 1.21 times the total amount of 300W ultrasonic extraction. Further increasing the ultrasonic power does not significantly improve the total amount of active substances extracted. When the ultrasonic power is 500W and the extraction time is 120min, the extraction equilibrium is reached. Even if the extraction time is extended, the total amount of active substances in the extract does not increase significantly. Therefore, it is preferred to set the extraction time to 120min and set the ultrasonic power to 500W.

[0132] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for crushing rhizomes of Artemisia sphaerocephala for extracting biologically active substances from Artemisia sphaerocephala, comprising a frame (1), characterized in that: A crushing box (2) is installed on the top of the frame (1), a crushing chamber (21) is opened on the crushing box (2), a powder hopper (7) is provided below the crushing box (2), a hopper (3) is fixedly installed through the top of the crushing box (2), a crushing mechanism (5) is provided in the crushing chamber (21), and a driving mechanism (4) is provided on the frame (1). After the rhizomes of Artemisia sphaerocephala are put into the hopper (3), they enter the crushing chamber (21), and then the driving mechanism (4) drives the crushing mechanism (5) to crush, cut and grind the rhizomes of Artemisia sphaerocephala, and the powder of the rhizomes of Artemisia sphaerocephala produced by grinding falls into the powder hopper (7); The pulverizing mechanism (5) comprises two rotating plates (51) rotatably mounted in the pulverizing chamber (21); two rotating columns (52) and two prisms (56) are rotatably mounted on the two rotating plates (51); the two rotating columns (52) and the two prisms (56) are staggered and arranged in a circular array; a plurality of linearly distributed cutting blades (54) are fixedly sleeved on the rotating columns (52); a grinding roller (58) is slidably sleeved on the prisms (56); two rotating disks (59) are rotatably mounted on the rotating plates (51); the two prisms (56) respectively penetrate corresponding rotating disks (59) and are fixedly connected to the corresponding rotating disks (59); A gear ring (55) is provided on the side of the two rotating plates (51) that is away from each other. The sides of the two gear rings (55) that are away from each other are respectively fixedly connected to the inner walls of the two sides of the pulverizing chamber (21). Gears 1 (53) are fixedly mounted on both ends of the rotating column (52). The two gears 1 (53) are respectively meshed and connected with the corresponding gear rings (55). Gears 2 (57) are fixedly mounted on both ends of the prism (56). The two gears 2 (57) are respectively meshed and connected with the corresponding gear rings (55). The driving mechanism (4) comprising a rotating shaft (41) penetrating through the crushing box (2) and rotatably connected to the crushing box (2), the rotating shaft (41) penetrating through two rotating plates (51) and fixedly connected to the two rotating plates (51), a motor (42) being fixedly mounted on one side of the frame (1), a second pulley (44) and a first pulley (43) being fixedly mounted on the end of the rotating shaft (41) and the output shaft of the motor (42), respectively, and the first pulley (43) and the second pulley (44) being sleeved with the same synchronous belt; Two arc-shaped filter plates (510) are fixedly mounted between the two rotating plates (51), the ends of the two arc-shaped filter plates (510) are slidably connected to the inner wall of the pulverizing chamber (21), and the two grinding rollers (58) are respectively located inside the corresponding arc-shaped filter plates (510). A discharge port (22) is provided at the bottom of the pulverizing chamber (21), and a discharge filter plate (23) is fixedly mounted inside the discharge port (22).

2. The device for crushing the rhizomes of Artemisia sphaerocephala for extracting biologically active substances from Artemisia sphaerocephala according to claim 1, characterized in that: A reciprocating mechanism (6) is provided on both sides of the grinding roller (58). The reciprocating mechanism (6) comprises two guide rods (61) fixedly mounted on the ends of the grinding roller (58) and two L-shaped rods (64) fixedly mounted on the side of the rotating plate (51) away from the grinding roller (58). The two L-shaped rods (64) are fixedly mounted with a same annular block (63) at the ends close to each other. The two guide rods (61) penetrate the rotating disk (59) and are slidably connected to the rotating disk (59). The two guide rods (61) are fixedly mounted with rollers (62) at the ends away from the grinding roller (58). The two rollers (62) are rollingly connected to the annular block (63).

3. The device for crushing the rhizomes of Artemisia sphaerocephala for extracting biologically active substances from Artemisia sphaerocephala according to claim 2, characterized in that: Two inner recesses (631) and two outer protrusions (632) are provided on one side of the annular block (63) close to the rotating disk (59); the two inner recesses (631) and the two outer protrusions (632) are staggered and connected end to end; and the roller (62) is adapted to the two inner recesses (631) and the two outer protrusions (632).

4. The device for crushing the rhizomes of Artemisia sphaerocephala for extracting biologically active substances from Artemisia sphaerocephala according to claim 1, characterized in that: The top of the pulverizing chamber (21) is provided with a feed port (24), the hopper (3) is fixedly connected to the inner wall of the feed port (24), the frame (1) is provided with a discharge port (11), the bottom of the pulverizing box (2) is fixedly connected to the top of the frame (1), and the discharge port (22) is connected to the discharge port (11), and a discharge pipe (12) connected to the discharge port (11) is fixedly installed at the bottom of the frame (1).

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