Method for preparing fertilizer containing auxin and prepared fertilizer

Through the bioconversion of insect larvae, ethylene polymers are converted into plant auxins, and environmentally friendly bioorganic fertilizers are prepared, which solves the problems of soil nutrient deficiency and chemical drug pollution, and realizes plant growth promotion and biological ethylene polymers. degradation.

CN119191885APending Publication Date: 2024-12-27ZHEJIANG SUB TROPICS CROP INST
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Patent Information

Application Number
CN202411377337.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-09-30
Publication Date
2024-12-27

AI Technical Summary

Technical Problem

The lack of natural nutrients in the soil has led to the inability to meet the needs of crop growth. The existing chemical and pharmaceutical plant growth regulators are not environmentally friendly and pollute the environment.

Method used

Using the bioconversion ability of insect larvae, ethylene polymers are converted into plant auxin, and bioorganic fertilizers rich in plant auxin are prepared through the excretion of insect larvae, reducing the use of chemical synthetic fertilizers and plant growth regulators.

Benefits of technology

The prepared fertilizer is environmentally friendly, can promote plant growth, reduce environmental pollution, has ecological and economic value, and realizes the biodegradation of ethylene polymers.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a method for preparing a fertilizer containing auxin and the prepared fertilizer, and relates to the technical field of fertilizer preparation, and the method comprises the following steps: adding an ethylene polymer into a basal feed for feeding insect larvae to obtain a compound feed; feeding the insect larvae with the compound feed; excrement of the insect larvae is collected; and obtaining the fertilizer by using the excrement. The fertilizer has the advantages of promoting plant growth and being good in environmental protection property.
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Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, and in particular to a method for preparing a fertilizer containing plant growth hormone and the prepared fertilizer. Background Art

[0002] The natural nutrient content in the soil is often limited and is affected by many factors, such as soil type, climatic conditions, crop types, and soil management practices, all of which may result in insufficient nutrient supply in the soil to meet the growth needs of crops. Therefore, in order to obtain high-yield and high-quality agricultural products, it is very important to use fertilizers appropriately and scientifically.

[0003] Auxin is a type of endogenous hormone containing an unsaturated aromatic ring and an acetic acid side chain, referred to as IAA in English. Its chemical nature is indoleacetic acid. The main effect of auxin on plant growth is to promote cell growth, especially cell elongation. It can also promote the development of fruits and the rooting of cuttings.

[0004] However, the synthesis of plant auxin is strictly controlled by many factors and has a typical polar distribution characteristic. There may be insufficient auxin content in the plant tissues and organs that people target. Therefore, people have developed chemical drugs such as auxin analogs as plant growth regulators to treat cash crops, but this method is not environmentally friendly and is easy to pollute the environment and affect sustainable development. Summary of the invention

[0005] The present invention aims to solve one of the technical problems in the related art to a certain extent. To this end, the present invention provides a method for preparing a fertilizer containing plant growth hormone and the fertilizer prepared therefrom, which has the advantages of promoting plant growth and being environmentally friendly.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] A method for preparing a fertilizer containing plant growth hormone comprises the following steps:

[0008] Adding ethylene polymers to a basic feed for feeding insect larvae to obtain a compound feed;

[0009] Feeding the insect larvae with the compound feed;

[0010] collecting excrement of the insect larvae;

[0011] The fertilizer is obtained by utilizing the excrement.

[0012] The present application provides a method for preparing a fertilizer containing auxin, which utilizes the biotransformation ability of some insect larvae, that is, some insect larvae can convert ethylene polymers (plastics) into auxins (i.e., IAA) and incorporate the auxins into their excrement, thereby preparing a biological organic fertilizer rich in auxins, which is environmentally friendly and can promote plant growth, while reducing the use of chemical synthetic fertilizers and chemical synthetic plant growth regulators, and promoting the biodegradation of ethylene polymers (plastics), and has important ecological and economic value.

[0013] Optionally, the ethylene polymer accounts for 20wt% to 50wt% in the compound feed.

[0014] Optionally, the basal feed comprises wheat bran.

[0015] Optionally, the basic feed further includes sucrose, and the proportion of the sucrose in the basic feed is 15wt% to 20wt%.

[0016] Optionally, the ethylene polymer includes polyethylene or polystyrene.

[0017] Optionally, the insect larvae include at least one of barleyworm larvae and mealworm larvae.

[0018] Optionally, in the step of feeding the insect larvae with the compound feed, the temperature of the environment where the insect larvae are located is 21° C. to 29° C.

[0019] Optionally, in the step of feeding the insect larvae with the compound feed, the relative humidity of the environment where the insect larvae are located is 65% to 75%.

[0020] In addition, the present invention also provides a fertilizer, which is prepared by the above method for preparing a fertilizer containing plant growth hormone. The beneficial effect reasoning process of the fertilizer containing plant growth hormone provided by the present invention is similar to that of the above method for preparing a fertilizer containing plant growth hormone, which will not be repeated here.

[0021] These features and advantages of the present invention will be disclosed in detail in the following specific embodiments and drawings. The best embodiments or means of the present invention will be described in detail in conjunction with the drawings, but they are not intended to limit the technical solutions of the present invention. In addition, there are multiple features, elements, and components that appear in each of the following texts and drawings, and different symbols or numbers are marked for convenience, but they all represent components with the same or similar structures or functions. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be further described below in conjunction with the accompanying drawings:

[0023] Figure 1The present invention is a schematic flow diagram of a method for preparing a fertilizer containing plant growth hormone. DETAILED DESCRIPTION

[0024] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments in the implementation manner are intended to be used to explain the present invention and should not be construed as limiting the present invention.

[0025] References in this specification to "one embodiment" or "an example" or "an example" mean that a particular feature, structure, or characteristic described in conjunction with the embodiment itself may be included in at least one embodiment of the present patent disclosure. The appearance of the phrase "in one embodiment" in various places in the specification does not necessarily refer to the same embodiment.

[0026] Example:

[0027] This embodiment provides a method for preparing a fertilizer containing plant growth hormone, such as Figure 1 As shown, the following steps are included:

[0028] S100, adding an ethylene polymer to a basic feed for feeding insect larvae to obtain a compound feed;

[0029] S200, feeding insect larvae with compound feed;

[0030] S300, collection of excrement of insect larvae;

[0031] S400, using the excrement to obtain fertilizer.

[0032] Auxin is an important substance that promotes plant growth, fruit development and cuttings. In this embodiment, a method for preparing a fertilizer containing auxin is provided, which utilizes the biotransformation ability of some insect larvae, that is, some insect larvae can convert ethylene polymers (plastics) into auxins (i.e., IAA) and incorporate the auxins into their excrement, thereby preparing a biological organic fertilizer rich in auxins, which is environmentally friendly and can promote plant growth, reduce the use of chemical synthetic fertilizers and chemical synthetic plant growth regulators, and promote the biodegradation of ethylene polymers (plastics), and has important ecological and economic value.

[0033] The reason why some insects can degrade ethylene polymers is that the bacteria in their intestines can decompose enzymes that destroy the structure of ethylene polymers, cut the chemical bonds of ethylene polymers, and decompose ethylene polymers for the synthesis of IAA and other organic matter, thereby achieving the degradation and utilization of ethylene polymers.

[0034] If the diet of insect larvae is completely replaced by ethylene polymers, although insects can temporarily maintain life activities, the growth of insects will be severely restricted due to the inability of ethylene polymers to provide comprehensive nutritional support, which may eventually endanger their survival. Therefore, in this embodiment, some ethylene polymers are added to the basic feed for feeding insect larvae to obtain a composite feed that can enable insect larvae to grow normally and produce excrement containing auxins. It should be noted that the basic feed refers to a feed that meets the basic nutritional needs of the selected insect larvae and is nutritionally comprehensive. It is sufficient to select feedable substances that can promote the growth, production and health of insect larvae and are safe and effective under reasonable use. The specific formula is not limited here.

[0035] The proportion of ethylene polymer in compound feed is 20wt% to 50wt%.

[0036] In this embodiment, the proportion of ethylene polymer in the compound feed is 20wt% to 50wt%. When the proportion of ethylene polymer in the compound feed is less than 20wt%, that is, the amount of ethylene polymer added is small, the insect larvae produce less auxin and the auxin content in the fertilizer is small; when the proportion of ethylene polymer in the compound feed is greater than 50wt%, the growth and development of the insects will be affected.

[0037] The basic feed consists of wheat bran.

[0038] The basic feed in the related art is mainly fermented cow dung, corn straw, sweet potato straw, etc., while in this embodiment, the basic feed includes wheat bran, which is a by-product in the grain processing process, mainly composed of the outer skin and germ of wheat, and rich in nutrients such as cellulose, protein, minerals and vitamins. The protein and cellulose in the wheat bran provide the necessary nutrients for the insects, which is beneficial to the growth and development of the insects and improves their physical fitness and immunity.

[0039] The basic feed also includes sucrose. In the basic feed, the proportion of sucrose is 15wt% to 20wt%.

[0040] In this embodiment, sucrose, as a sugar substance, can increase the sugar content in the insect larvae, help promote the growth and development of insects, and support their daily activities and growth needs. In addition, sucrose has a sweet taste, and adding an appropriate amount to the feed can increase the palatability of the feed, thereby increasing the appetite and food intake of insect larvae, which is of great significance for the healthy growth and weight gain of insect larvae. However, the addition of sucrose will affect the insects' feeding on ethylene polymers, so the addition amount should be strictly controlled.

[0041] Vinyl polymers include polyethylene and / or polystyrene.

[0042] The applicant has found through research that insect larvae prefer to eat polystyrene plastics and polyethylene plastics, and polystyrene plastics and polyethylene plastics are difficult to degrade in the natural environment, resulting in serious environmental pollution problems. Therefore, degrading polyethylene and polystyrene by insect larvae is beneficial to environmental protection. Specifically, polystyrene foam and polyethylene plastic film are added to the basic feed to facilitate insects to gnaw.

[0043] The insect larvae include at least one of a barleyworm larvae and a yellow mealworm larvae.

[0044] Insects such as mealworms and yellow mealworms have a certain ability to feed on plastics due to their omnivorous characteristics. Therefore, in this embodiment, mealworms and / or yellow mealworms are selected. The applicant has found that yellow mealworms and mealworms can feed on plastics and produce feces containing plant growth hormones after feeding on plastics. This characteristic has a significant contribution to the biological treatment of waste plastics. The larvae of mealworms and yellow mealworms can degrade or transform ethylene polymers through their unique digestive mechanism, thereby reducing the pollution of plastic waste to the environment. In addition, the adult worms of yellow mealworms and mealworms have a small food intake and a short life cycle, so the larvae of mealworms and yellow mealworms are selected in this embodiment.

[0045] In addition, mealworms and barley worms are now common farmed insects. They play an important role in the breeding industry due to their high nutritional value and wide range of uses. Mealworm larvae are rich in protein and can be used to produce animal protein feed after breeding.

[0046] In this embodiment, insect excrement can be used directly as fertilizer, or as fertilizer after being air-dried, without the need for fermentation treatment, while conventional organic fertilizers such as sheep manure and chicken manure need to be fermented.

[0047] In other embodiments, the excrement may also be fermented, and the high temperature generated during the fermentation process can kill harmful microorganisms such as pathogens and parasite eggs in the excrement, thereby reducing the biosafety risk when using fertilizers.

[0048] In the step of feeding the insect larvae with the compound feed, the temperature of the environment where the insect larvae are located is 21° C. to 29° C., and the relative humidity of the environment where the insect larvae are located is 65% to 75%.

[0049] In this embodiment, the environment temperature of the insect larvae is 21°C to 29°C. At this time, the metabolic activity of the insects is most active, and they can more effectively digest and absorb the nutrients in the feed, thereby promoting their rapid growth and development. If the ambient temperature is too low, the metabolic activity of the insects will slow down, and the growth rate will also decrease accordingly; if the ambient temperature is too high, it may cause the water in the insects to evaporate too quickly, resulting in dehydration, and even death in severe cases. For barley worm larvae and yellow mealworm larvae, the suitable relative humidity range is usually 65% ​​to 75%. Within this humidity range, insects can maintain the balance of water in their bodies, avoid dehydration caused by low humidity, and also prevent problems such as mold growth caused by high humidity.

[0050] This embodiment also provides a fertilizer, which is prepared by the aforementioned method for preparing a fertilizer containing plant growth hormone.

[0051] The fertilizer in this embodiment is rich in auxin, which can promote the growth and development of plants and improve the yield and quality of crops. Using the excrement of insect larvae as raw materials not only realizes the resource utilization of ethylene polymers, but also reduces environmental pollution. At the same time, this method is sustainable, and insect larvae can continue to reproduce and produce more excrement as fertilizer raw materials.

[0052] The content of plant growth hormone in the fertilizer is 3 to 60 μg / g.

[0053] In this embodiment, when preparing fertilizer containing auxin, the content of auxin is controlled to be 3-60 μg / g. Too low auxin content may not achieve the expected growth promotion effect, while too high content may inhibit plant growth.

[0054] Preparation Example 1:

[0055] 350 g of wheat bran and 150 g of polyethylene plastic film were weighed, crushed and mixed evenly to make a compound feed, 100 yellow mealworm larvae of similar age and size were selected and placed in a constant temperature incubator for cultivation, the compound feed was placed in the constant temperature incubator for the yellow mealworm larvae to eat, the excrement of the yellow mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0056] Preparation Example 2:

[0057] 350 g of wheat bran and 150 g of polystyrene foam were weighed, crushed and mixed evenly to make a compound feed, 100 yellow mealworm larvae of similar age and size were selected and placed in a constant temperature incubator for cultivation, the compound feed was placed in the constant temperature incubator for the yellow mealworm larvae to eat, the excrement of the yellow mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0058] Preparation Example 3:

[0059] 300 g of wheat bran, 50 g of sucrose and 150 g of polystyrene foam were weighed, crushed and mixed evenly to make a compound feed, 100 yellow mealworm larvae of similar age and size were selected and cultured in a constant temperature incubator, the compound feed was placed in the constant temperature incubator for the yellow mealworm larvae to eat, the excrement of the yellow mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0060] Preparation Example 4:

[0061] 350 g of wheat bran and 150 g of polyethylene plastic film were weighed, crushed and mixed evenly to make a compound feed, 100 mealworm larvae of similar age and size were selected and cultured in a constant temperature incubator, the compound feed was placed in the constant temperature incubator for the mealworm larvae to feed, the excrement of the mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0062] Preparation Example 5:

[0063] 350 g of wheat bran and 150 g of polystyrene foam were weighed, crushed and mixed evenly to make a compound feed, 100 mealworm larvae of similar age and size were selected and cultured in a constant temperature incubator, the compound feed was placed in the constant temperature incubator for the mealworm larvae to feed, the excrement of the mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0064] Preparation Example 6:

[0065] 300 g of wheat bran, 50 g of sucrose and 150 g of polystyrene foam were weighed, crushed and mixed evenly to make a compound feed, 100 larvae of similar age and size were selected and placed in a constant temperature incubator for cultivation, the compound feed was placed in the constant temperature incubator for the larvae to eat, the excrement of the larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0066] Comparative Example 1:

[0067] 500 g of wheat bran was weighed, 100 mealworm larvae of similar age and size were selected and placed in a constant temperature incubator for cultivation, the wheat bran was placed in the constant temperature incubator for the mealworm larvae to feed on, the excrement of the mealworm larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0068] Comparative Example 2:

[0069] 500 g of wheat bran was weighed, 100 larvae of similar age and size were selected and placed in a constant temperature incubator for cultivation, the wheat bran was placed in the constant temperature incubator for the larvae to feed on, the excrement of the larvae was collected and the content of auxin in the excrement was detected. The test results are shown in Table 1.

[0070] Table 1: The content of auxin (IAA) in the excrement of insect larvae in the preparation examples and comparative examples.

[0071] serial number IAA content (μg / g) Preparation Example 1 77.6736±1.7705 Preparation Example 2 10.8504±0.4985 Preparation Example 3 3.7715±0.1964 Preparation Example 4 10.3311±0.6978 Preparation Example 5 11.0657±0.6402 Preparation Example 6 4.3935±0.1291 Comparative Example 1 5.1919±0.1858 Comparative Example 2 6.5691±0.5339

[0072] Comparing Preparation Example 1 (wheat bran + polyethylene plastic film) with Preparation Example 2 (wheat bran + polystyrene foam), and Preparation Example 3 (adding sucrose), it can be seen that there are significant differences in the content of auxin. The IAA content in the excrement of yellow mealworm larvae in Preparation Example 1 is the highest (77.6736μg / g), while the content is significantly reduced in Preparation Examples 2 and 3, which shows that the combination of wheat bran and polyethylene plastic film is more effective in promoting yellow mealworms to produce more auxin. Comparing Preparation Example 2 (without sucrose) with Preparation Example 3 (containing sucrose), it can be seen that after adding sucrose, the IAA content in the excrement of yellow mealworm larvae is further reduced. Sucrose may have changed the metabolic pathway of yellow mealworm larvae, reduced the generation of metabolites related to the production of auxin, or affected the utilization efficiency of other ingredients in the feed by yellow mealworm larvae. The IAA content in the excrement of yellow mealworm larvae in Preparation Example 1 is much higher than that in Comparative Example 1. This shows that after adding polyethylene plastic film to the feed, yellow mealworm larvae can produce more auxin.

[0073] Comparing Preparation Example 1 with Preparation Example 4 (both wheat bran + polyethylene plastic film), and Preparation Example 2 with Preparation Example 5 (both wheat bran + polystyrene foam), it can be seen that the IAA content in the excrement of the barleyworm larvae is significantly lower than that of the mealworm larvae. This shows that different types of insects have different auxin contents in their excrement under the same feed conditions, and mealworms have more advantages.

[0074] The IAA content in the excrement of the mealworm larvae in Preparation Example 4 is much higher than that in Comparative Example 2. This indicates that after adding polyethylene plastic film to the feed, the mealworm larvae can produce more auxin.

[0075] The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that the present invention includes but is not limited to the contents described in the drawings and the above specific embodiments. Any modification that does not deviate from the functional and structural principles of the present invention will be included in the scope of the claims.

Claims

1. A method for preparing a fertilizer containing plant growth hormone, characterized in that: The steps include: Adding ethylene polymers to a basic feed for feeding insect larvae to obtain a compound feed; Feeding the insect larvae with the compound feed; collecting excrement of the insect larvae; The fertilizer is obtained by utilizing the excrement.

2. The method according to claim 1, characterized in that The ethylene polymer accounts for 20 wt % to 50 wt % in the compound feed.

3. The method according to claim 1, characterized in that: The basal feed includes wheat bran.

4. The method according to claim 3, characterized in that The basic feed also includes sucrose, and in the basic feed, the proportion of the sucrose is 15wt% to 20wt%.

5. The method according to claim 1, characterized in that The ethylene polymer includes polyethylene or polystyrene.

6. The method according to any one of claims 1 to 5, characterized in that: The insect larvae include at least one of barleyworm larvae and yellow mealworm larvae.

7. The method according to any one of claims 1 to 5, characterized in that: In the step of feeding the insect larvae with the compound feed, the temperature of the environment where the insect larvae are located is 21° C. to 29° C.

8. The method according to any one of claims 1 to 5, characterized in that: In the step of feeding the insect larvae with the compound feed, the relative humidity of the environment where the insect larvae are located is 65% to 75%.

9. A fertilizer, characterized in that: The fertilizer is prepared by the method for preparing a fertilizer containing plant growth hormone according to any one of claims 1 to 8.

10. A fertilizer according to claim 9, characterized in that: The content of plant growth hormone in the fertilizer is 3-60 μg / g.