Microbial preparation for promoting corn growth and preparation method thereof

Abstract

The invention relates to the technical field of microorganisms, and provides a microbial preparation for promoting corn growth and a preparation method, and the microbial preparation comprises the following components in parts by weight: 5-7.5 parts of a complex microbial inoculant, 12-24 parts of bean pulp powder, 6-9 parts of cottonseed meal, 7-10 parts of corn flour, 1-1.5 parts of glucose, 1-3 parts of amino acid, 3-7 parts of magnesium sulfate, 4.5-6 parts of fish protein and 30-45 parts of water. A carrier is prepared from sodium alginate and a calcium chloride cross-linking agent to wrap compound bacteria of bacillus amyloliquefaciens and paecilomyces lilacinus, so that the compound bacteria are stable in structure and resistant to high temperature, harm of an existing chemical fertilizer to soil is greatly reduced, and meanwhile, the compound bacteria have a good growth promoting effect on corn.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a microbial preparation for promoting corn growth and a preparation method thereof. Background Art

[0002] Corn is one of China's main food crops. At present, the cultivation and management of corn mainly includes the main production links such as corn variety selection, seed treatment, land preparation, sowing, field management, etc. The existing chemical fertilizer application technology and crop root strengthening and nourishing technology can make corn plants grow vigorously; or use corn seeds to coat to achieve the healthy germination requirements of insect, fungus and anti-corrosion, and apply chemical fertilizers to prevent the plants from being weak and falling over and increase the width of the corn leaves, thereby reducing plant lodging and increasing plant photosynthesis.

[0003] Fertilizer is the food of crops. As farmers' demands for high-quality, special fertilizers and fertilizers that can improve crop resistance and immunity become increasingly strong, the research and development of comprehensive and multifunctional fertilizers is becoming more and more urgent. At present, compound fertilizers are the main special fertilizers in the agricultural supply market. There are very few green special biological fertilizer products that meet the specific needs of crops, and there is a serious lack of customized special biological fertilizer products. Faced with the increasingly fragile agricultural ecological environment, screening microbial strains with specific functions and, based on this, developing new biological agents and biological fertilizers with special functions for specific crops, nutritional needs, soil properties and disease prevention and control are key technologies that need to be overcome in current agricultural production.

[0004] Affected by the current planting model, the long-term use of existing microbial fertilizers, the persistent high-density continuous cropping, and the lack of soil maintenance have led to long-term adverse effects on the soil's microbial flora. The death of beneficial bacteria in the soil and the massive reproduction of pathogens have greatly increased the risk of crop diseases and insect pests. The crop root system lacks functional beneficial microorganisms and lacks the inhibition and antagonism of the proliferation of harmful bacteria, which increases the degree and frequency of crop disease. Most of the cultivated soils in the country, especially greenhouse soils, lack beneficial bacteria, and the plant roots are shallow and cannot absorb water and nutrients well. The abuse of chemical fertilizers harms agricultural soils, and the use of chemical fertilizers needs to be reduced. In this way, not only has the yield of corn not increased, but it has also been impossible to continue to increase the yield of corn at a low cost.

[0005] Patent publication number CN113980835A discloses a composite microbial agent and its application in fertilizers. It uses Bacillus megaterium, Bacillus velez and Bacillus subtilis. Although it improves the problems of singleness and soil, the preparation steps are cumbersome and the conditions are relatively complicated. There is still a need for more improvements in composite microbial preparations.

[0006] Therefore, the technology of microbial preparations and preparation methods for promoting corn growth in the prior art still needs to be further improved. Summary of the invention

[0007] In view of this, the present invention proposes a microbial preparation for promoting corn growth and a preparation method, which solves the technical problems of poor activity and poor fertilizer efficiency in the prior art, and high harm of chemical fertilizers to the soil.

[0008] The technical solution of the present invention is achieved in this way:

[0009] On the one hand, the present invention provides a microbial preparation for promoting corn growth, comprising the following parts by weight: 5-7.5 parts of a composite bacterial agent, 12-24 parts of soybean meal powder, 6-9 parts of cottonseed meal, 7-10 parts of corn meal, 1-1.5 parts of glucose, 1-3 parts of amino acids, 3-7 parts of magnesium sulfate, 4.5-6 parts of fish protein and 30-45 parts of water.

[0010] On the basis of this technical solution, it is further preferred that the composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 5-7%.

[0011] On the basis of this technical solution, it is further preferred that the preparation method of the composite bacterial agent specifically comprises the following steps:

[0012] After culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus respectively, centrifuging, diluting, and mixing with 5-8% by mass sodium alginate at a volume ratio of 1:1-2.1 to obtain a mixture;

[0013] The mixture is dropped into calcium chloride with a mass fraction of 5-7% to obtain the composite bacterial agent.

[0014] On the basis of this technical solution, further preferably, the dilution includes diluting to OD 600 After the pH value reaches 0.4-0.6, mix with sodium alginate.

[0015] On the basis of this technical solution, further preferably, the culture conditions of the Bacillus amyloliquefaciens are: NB medium, 29-30° C., 140-160 r / min shaking culture for 22-28 h.

[0016] On the basis of this technical solution, further preferably, the culture conditions of the Paecilomyces lilacinus are: improved culture medium, 27-29° C., 130-140 r / min shaking culture for 30-48 h.

[0017] On the basis of this technical solution, it is further preferred that the improved culture medium has the following components: each gram of corn cob bran includes 2-3% lactose, 1-2% peptone, 0.3-0.5% sodium nitrate, 0.05-0.07% zinc chloride and 10-12 ml water.

[0018] In a second aspect, the present invention further provides a method for preparing the microbial preparation for promoting corn growth according to the first aspect, comprising the following steps:

[0019] S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use;

[0020] S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture under ventilation for 22-30 hours to obtain the microbial preparation.

[0021] On the basis of this technical solution, more preferably, the stirring speed is 145-155r / min.

[0022] On the basis of this technical solution, more preferably, the fermentation specifically includes a steam temperature of 120-135°C for 30-40 min, and then transferred to 25-29°C for cultivation.

[0023] Compared with the prior art, the microbial preparation for promoting corn growth and its application provided by the present invention have the following beneficial effects:

[0024] By identifying two composite bacteria of Bacillus amyloliquefaciens and Paecilomyces lilacinus, and encapsulating them with a carrier prepared by sodium alginate and a cross-linking agent, a composite bacterial agent with excellent growth-promoting ability and no antagonism was obtained. The prepared composite bacterial agent has a significant growth-promoting effect on corn root length, root volume and surface area, and is an environmentally friendly material that is harmless to the environment, plants and microorganisms.

[0025] The composite bacterial agent includes Bacillus amyloliquefaciens and Paecilomyces lilacinus. The two bacteria and the biological enzymes secreted by them constitute an efficient synergistic system, which can effectively improve the efficiency of fermentation. At the same time, the improved culture medium provided by the invention and the corncob chaff not only effectively utilize the existing agricultural waste, but also zinc chloride can provide good inorganic salt and nutrient supplements therefor, and the method is simple.

[0026] The microbial preparation for promoting corn growth provided by the present invention has good activity, can meet the nutritional needs of corn, improve crop soil, and increase yield and quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1It is a schematic diagram of the growth of corn roots described in Examples 1-4 and Comparative Examples 1-5 of the present invention;

[0029] Figure 2 It is a schematic diagram of the growth of the total surface area of ​​corn roots described in Examples 1-4 and Comparative Examples 1-5 of the present invention;

[0030] Figure 3 It is a schematic diagram of the growth of the corn root volume described in Examples 1-4 of the present invention and Comparative Examples 1-5. DETAILED DESCRIPTION

[0031] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The present invention provides a microbial preparation for promoting corn growth and a preparation method thereof. The carrier is prepared by using sodium alginate and calcium chloride crosslinking agents to encapsulate a composite fungus of Bacillus amyloliquefaciens and Paecilomyces lilacinus. The carrier has a stable structure and is resistant to high temperatures. The harmfulness of existing chemical fertilizers to the soil is greatly reduced, and the carrier has a good growth-promoting effect on corn.

[0033] Example 1

[0034] A microbial preparation for promoting corn growth comprises the following parts by weight: 5g of a composite bacterial agent, 12g of soybean meal powder, 6g of cottonseed meal, 7g of corn meal, 1g of glucose, 1g of amino acids, 3g of magnesium sulfate, 4.5g of fish protein and 30g of water.

[0035] In this embodiment, specifically, the composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 5%.

[0036] In this embodiment, specifically, the preparation method of the composite bacterial agent comprises the following steps:

[0037] Bacillus amyloliquefaciens and Paecilomyces lilacinus were cultured separately, centrifuged, diluted, and mixed with 5% sodium alginate in a volume ratio of 1:1 to obtain a mixture;

[0038] The mixture is dropped into calcium chloride with a mass fraction of 5% to obtain the composite bacterial agent.

[0039] In this embodiment, specifically, the dilution includes diluting to OD 600 After the pH value reaches 0.4, mix with sodium alginate.

[0040] In this embodiment, specifically, the culture conditions of Bacillus amyloliquefaciens are: NB medium, 29° C., 140 r / min shaking culture for 28 h.

[0041] In this embodiment, specifically, the culture conditions of the Paecilomyces lilacinus are: improved culture medium, shaking culture at 27° C. and 130 r / min for 48 h.

[0042] In this embodiment, specifically, the improved culture medium has the following components: each gram of corn cob bran includes 2% lactose, 1% peptone, 0.3% sodium nitrate, 0.05% zinc chloride and 10 ml water.

[0043] The method for preparing the microbial preparation for promoting corn growth described in this embodiment comprises the following steps:

[0044] S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use;

[0045] S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture with ventilation for 30 hours to obtain the microbial preparation.

[0046] More specifically, the stirring speed is 145 r / min.

[0047] More specifically, the fermentation includes a steam temperature of 120° C. for 40 minutes and then transferred to 25° C. for cultivation.

[0048] Example 2

[0049] A microbial preparation for promoting corn growth comprises the following parts by weight: 7.5 g of a composite bacterial agent, 24 g of soybean meal powder, 9 g of cottonseed meal, 10 g of corn meal, 1.5 g of glucose, 3 g of amino acids, 7 g of magnesium sulfate, 6 g of fish protein and 45 g of water.

[0050] In this embodiment, specifically, the composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 7%.

[0051] In this embodiment, specifically, the preparation method of the composite bacterial agent comprises the following steps:

[0052] Bacillus amyloliquefaciens and Paecilomyces lilacinus were cultured separately, centrifuged, diluted, and mixed with 8% sodium alginate in a volume ratio of 1:2.1 to obtain a mixture;

[0053] The mixture is dropped into calcium chloride with a mass fraction of 7% to obtain the composite bacterial agent.

[0054] In this embodiment, specifically, the dilution includes diluting to OD 600 After the pH value reaches 0.6, mix with sodium alginate.

[0055] In this embodiment, specifically, the culture conditions of Bacillus amyloliquefaciens are: NB medium, 30° C., 160 r / min shaking culture for 22 h.

[0056] In this embodiment, specifically, the culture conditions of the Paecilomyces lilacinus are: improved culture medium, shaking culture at 29° C. and 140 r / min for 30 h.

[0057] In this embodiment, specifically, the improved culture medium has the following components: each gram of corn cob bran includes 3% lactose, 2% peptone, 0.5% sodium nitrate, 0.07% zinc chloride and 12 ml of water.

[0058] The method for preparing the microbial preparation for promoting corn growth described in this embodiment comprises the following steps:

[0059] S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use;

[0060] S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture under ventilation for 22 hours to obtain the microbial preparation.

[0061] More specifically, the stirring speed is 155 r / min.

[0062] More specifically, the fermentation includes a steam temperature of 135° C. for 30 minutes and then transferred to 29° C. for cultivation.

[0063] Example 3

[0064] A microbial preparation for promoting corn growth comprises the following parts by weight: 6g of a composite bacterial agent, 18g of soybean meal powder, 7g of cottonseed meal, 8.5g of corn meal, 1.3g of glucose, 2g of amino acids, 5g of magnesium sulfate, 5g of fish protein and 42g of water.

[0065] In this embodiment, specifically, the composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 6%.

[0066] In this embodiment, specifically, the preparation method of the composite bacterial agent comprises the following steps:

[0067] Bacillus amyloliquefaciens and Paecilomyces lilacinus were cultured separately, centrifuged, diluted, and mixed with 6.5% sodium alginate in a volume ratio of 1:1.5 to obtain a mixture;

[0068] The mixture is dropped into calcium chloride with a mass fraction of 6% to obtain the composite bacterial agent.

[0069] In this embodiment, specifically, the dilution includes diluting to OD 600 After the concentration is 0.5, mix with sodium alginate.

[0070] In this embodiment, specifically, the culture conditions of Bacillus amyloliquefaciens are: NB medium, 29° C., 150 r / min shaking culture for 26 h.

[0071] In this embodiment, specifically, the culture conditions of the Paecilomyces lilacinus are: improved culture medium, 28° C., and shaking culture at 135 r / min for 38 h.

[0072] In this embodiment, specifically, the improved culture medium has the following components: each gram of corn cob bran includes 2.5% lactose, 1.5% peptone, 0.4% sodium nitrate, 0.06% zinc chloride and 11 ml of water.

[0073] The method for preparing the microbial preparation for promoting corn growth described in this embodiment comprises the following steps:

[0074] S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use;

[0075] S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture under ventilation for 26 hours to obtain the microbial preparation.

[0076] More specifically, the stirring speed is 150 r / min.

[0077] More specifically, the fermentation includes a steam temperature of 125° C. for 35 minutes and then transferred to 28° C. for cultivation.

[0078] Example 4

[0079] A microbial preparation for promoting corn growth comprises the following parts by weight: 5g of a composite bacterial agent, 12g of soybean meal powder, 6g of cottonseed meal, 7g of corn meal, 1g of glucose, 1g of amino acids, 3g of magnesium sulfate, 4.5g of fish protein and 30g of water.

[0080] In this embodiment, specifically, the composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 6%.

[0081] In this embodiment, specifically, the preparation method of the composite bacterial agent comprises the following steps:

[0082] Bacillus amyloliquefaciens and Paecilomyces lilacinus were cultured separately, centrifuged, diluted, and mixed with 7% sodium alginate in a volume ratio of 1:1.5 to obtain a mixture;

[0083] The mixture is dropped into calcium chloride with a mass fraction of 6% to obtain the composite bacterial agent.

[0084] In this embodiment, specifically, the dilution includes diluting to OD 600 After the concentration is 0.5, mix with sodium alginate.

[0085] In this embodiment, specifically, the culture conditions of Bacillus amyloliquefaciens are: NB medium, 30° C., shaking culture at 150 r / min for 25 h.

[0086] In this embodiment, specifically, the culture conditions of the Paecilomyces lilacinus are: improved culture medium, 28° C., and shaking culture at 135 r / min for 38 h.

[0087] In this embodiment, specifically, the improved culture medium has the following components: each gram of corn cob bran includes 2.5% lactose, 1.5% peptone, 0.4% sodium nitrate, 0.06% zinc chloride and 11 ml of water.

[0088] The method for preparing the microbial preparation for promoting corn growth described in this embodiment comprises the following steps:

[0089] S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use;

[0090] S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture under ventilation for 28 hours to obtain the microbial preparation.

[0091] More specifically, the stirring speed is 145 r / min.

[0092] More specifically, the fermentation includes a steam temperature of 120° C. for 30 minutes and then transferred to 29° C. for cultivation.

[0093] Comparative Example 1

[0094] A microbial preparation for promoting corn growth, which is different from Example 1 in that no composite bacterial agent is added.

[0095] Comparative Example 2

[0096] A microbial preparation for promoting corn growth, which is different from Example 2 in that Bacillus amyloliquefaciens is not added to the composite bacterial preparation.

[0097] Comparative Example 3

[0098] A microbial preparation for promoting corn growth, which is different from Example 3 in that no Paecilomyces lilacinus is added to the composite bacterial preparation.

[0099] Comparative Example 4

[0100] A microbial preparation for promoting corn growth, which is different from Example 4 in that Bacillus amyloliquefaciens and Paecilomyces lilacinus in the composite microbial preparation are not mixed with sodium alginate but are directly cultured.

[0101] Comparative Example 5

[0102] A microbial preparation for promoting corn growth, which is different from Example 4 in that the improved culture medium of Paecilomyces lilacinus is Czapek's medium.

[0103] Growth effect test

[0104] Select corn seeds with full grains and uniform size, rinse, soak in alcohol for 5 min, and disinfect. Then put the seeds in a filter paper tray, add 10 mL of sterile water, and culture in the dark to allow the corn seeds to germinate. Select corn seeds with consistent sprout length and transplant them into potting soil, and apply 20 mL of the bacterial agent of the above embodiment and control example respectively. Cultivate the corn at room temperature of 25°C for 15 days, determine various nutritional indicators, and use a root scanner (Top Yunnong GXY-A) to measure the root length, root surface area and root volume of the corn. The specific results are as follows: Figure 1-3 shown.

[0105] Table 1 Detection of nutritional indexes of corn

[0106]

[0107] The results are shown in Table 1 and Figure 1-3 It can be seen that this shows that the composite bacterial agent has a significant growth-promoting effect on the root system of corn, and the growth-promoting effect of total root length and root surface area is better than that of the comparative example, and the nutritional components of calcium, potassium and zinc in Examples 1-4 are better than those in Comparative Examples 1-5, and the protein and insoluble fiber are also slightly better than those in Comparative Examples, but the differences are not significant. At the same time, it was found that without using the improved culture medium, the growth of corn in Comparative Example 5 was slightly worse than that in the examples, but better than Comparative Examples 1-4. The reason may be that the existing Cha's culture medium failed to effectively supplement the nutrients such as inorganic salts required by Paecilomyces lilacinus compared with the improved culture medium.

[0108] In summary, the present invention provides a microbial preparation and preparation method for promoting corn growth. The carrier is prepared by using sodium alginate and calcium chloride crosslinking agent to encapsulate the composite bacteria of Bacillus amyloliquefaciens and Paecilomyces lilacinus. Not only is the structure stable and high temperature resistant, but it also greatly reduces the harmfulness of existing chemical fertilizers to the soil, and has a good growth-promoting effect on corn. Compared with existing microbial fertilizers, it can better improve the soil environment, promote root growth, increase corn yield and quality, and promote green agricultural development.

[0109] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A microbial preparation for promoting corn growth, characterized in that: The invention comprises the following parts by weight: 5-7.5 parts of composite bacterial agent, 12-24 parts of soybean meal powder, 6-9 parts of cottonseed meal, 7-10 parts of corn meal, 1-1.5 parts of glucose, 1-3 parts of amino acid, 3-7 parts of magnesium sulfate, 4.5-6 parts of fish protein and 30-45 parts of water; The composite bacterial agent is prepared by culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus, mixing with sodium alginate, and then dropping into calcium chloride with a mass fraction of 5-7%.

2. The microbial preparation for promoting corn growth according to claim 1, characterized in that: The preparation method of the composite bacterial agent specifically comprises the following steps: After culturing Bacillus amyloliquefaciens and Paecilomyces lilacinus respectively, centrifuging, diluting, and mixing with 5-8% by mass sodium alginate at a volume ratio of 1:1-2.1 to obtain a mixture; The mixture is dropped into calcium chloride with a mass fraction of 5-7% to obtain the composite bacterial agent.

3. The microbial preparation for promoting corn growth according to claim 2, characterized in that: The dilution includes diluting to OD 600 After the pH value reaches 0.4-0.6, mix with sodium alginate.

4. The microbial preparation for promoting corn growth according to claim 2, characterized in that: The culture conditions of the Bacillus amyloliquefaciens are: NB medium, 29-30° C., 140-160 r / min shaking culture for 22-28 hours.

5. The microbial preparation for promoting corn growth according to claim 2, characterized in that: The culture conditions of the Paecilomyces lilacinus are: improved culture medium, 27-29° C., 130-140 r / min shaking culture for 30-48 hours.

6. The microbial preparation for promoting corn growth according to claim 5, characterized in that: The improved culture medium has the following components: each gram of corncob bran contains 2-3% lactose, 1-2% peptone, 0.3-0.5% sodium nitrate, 0.05-0.07% zinc chloride and 10-12 ml water.

7. The method for preparing the microbial preparation for promoting corn growth according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Sterilize soybean meal, cottonseed meal, corn meal, magnesium sulfate, glucose, amino acids and fish protein, mix thoroughly, dry and obtain a matrix for later use; S2. Add the composite bacterial agent and water to the matrix obtained in step S1, stir evenly, ferment, and culture under ventilation for 22-30 hours to obtain the microbial preparation.

8. The method for preparing the microbial preparation for promoting corn growth according to claim 7, characterized in that: The stirring speed is 145-155r / min.

9. The method for preparing the microbial preparation for promoting corn growth according to claim 7, characterized in that: The fermentation specifically includes a steam temperature of 120-135° C. for 30-40 minutes, and then transferred to 25-29° C. for cultivation.

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