Blood polymerization parameter detector

Abstract

According to the blood polymerization parameter detector, when a second depolymerization module is connected with the first end of a first depolymerization module through a third pipeline module, the sum of the capacity of a first depolymerization module and the capacity of the first pipeline module is larger than 150 microliters, and the capacity of the first depolymerization module is larger than 80 microliters; the capacity ratio of the second depolymerization module to the first depolymerization module is greater than or equal to 5 and less than or equal to 50; or when the second depolymerization module is connected with the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacities of the second depolymerization module and the first depolymerization module is greater than or equal to 10 and less than or equal to 100. Based on the mode, the reliability of the blood polymerization parameter detector can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of sample detection, and in particular to a blood polymerization parameter detector. Background Art

[0002] Blood contains many types of particles, such as exosomes, hemoglobin, platelets, red blood cells, white blood cells, etc., and each type of particle also contains various subtypes. Due to the diversity of chemical substances and physical states on various particles, they can be regarded as a suspension under a multiphase physical field. In a relatively static state, various types (or subtypes) of cells tend to aggregate together, and the differences in aggregation parameters of various blood cells often reveal different pathological states.

[0003] However, in the process of blood testing, multiple blood samples (often corresponding to multiple patients) are often collected at different times, places, and methods, and then tested in batches after being collected together. Their relative static time, state, and influencing factors are different; and the order of batch testing further seriously damages the consistency of environmental factors between different samples when testing the aggregation parameters of batch samples.

[0004] Existing blood polymerization parameter detectors are usually only able to aspirate blood samples for polymerization parameter detection. When testing different batches of samples, even if they are the same blood samples, the results of the polymerization parameter detection will be different due to the differences in relative static time, state, and influencing factors. In other words, the detection results of existing blood polymerization parameter detectors are easily distorted and have poor reliability. Summary of the invention

[0005] The main technical problem solved by this application is how to improve the reliability of a blood polymerization parameter detector.

[0006] In order to solve the above technical problems, the technical solution adopted in the present application is: a blood polymerization parameter detector, comprising: a sample preparation module, the sample preparation module is used to prepare a blood sample to be tested; a first depolymerization module, the first end of the first depolymerization module is connected to the sample preparation module; a first pipeline module, the second end of the first depolymerization module is connected to the first end of the first pipeline module; a second depolymerization module, the second end of the first pipeline module is connected to the first end of the second depolymerization module; a second pipeline module, the second end of the second depolymerization module is connected to the first end of the second pipeline module; a first depolymerization module, the second end of the second pipeline module is connected to the first end of the first depolymerization module; a third pipeline module; a second depolymerization module, the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module; a polymerization parameter detection module, a polymerization The parameter detection module is arranged on the first pipeline module or the second pipeline module, and the polymerization parameter detection module is used to detect the polymerization parameter of the blood sample in the first pipeline module or the second pipeline module; when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 5 and less than or equal to 50, or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 10 and less than or equal to 100.

[0007] When the first deaggregation module and / or the second deaggregation module sucks out the blood sample, the maximum displacement of one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module, and / or the blood sample passes through the first pipeline module, and / or the blood sample does not pass through the second deaggregation module.

[0008] Wherein, the opening direction of one end of the first deagglomeration module is different from the opening direction of the other end of the first deagglomeration module; and / or, the opening direction of one end of the second deagglomeration module is different from the opening direction of the other end of the second deagglomeration module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the first deagglomeration module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the second deagglomeration module; and / or, the minimum tube diameter of the second deagglomeration module is different from the tube diameter of the first pipeline module; and / or, the minimum tube diameter of the second deagglomeration module is different from the tube diameter of the second pipeline module; and / or, the maximum tube diameter of the second deagglomeration module is different from the tube diameter of the first pipeline module; and / or, the maximum tube diameter of the second deagglomeration module is different from the tube diameter of the second pipeline module; and / or, the tube length of the first pipeline module is greater than or equal to 5 cm.

[0009] Among them, the extension direction of the inner cavity of the sample preparation module is parallel to the extension direction of the inner cavity of the first pipeline module, and the distance between the projection of the sample preparation module on the horizontal plane and the projection of the first pipeline module on the horizontal plane is less than 6 cm; and / or, the distance between the projection of one end of the first deagglomeration module on the horizontal plane and the projection of the other end of the first deagglomeration module on the horizontal plane is less than 6 cm.

[0010] Among them, the first pipeline module includes a first sub-pipeline module and a second sub-pipeline module which are connected to each other; the two ends of the first sub-pipeline module are relatively fixed, keeping the inner cavity axis of the first sub-pipeline module as a straight line perpendicular to the horizontal plane; one end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second deagglomeration module, and the second sub-pipeline module can perform corresponding deformation and / or movement when one end of the second sub-pipeline module and the other end of the second sub-pipeline module undergo relative movement.

[0011] Among them, when the polymerization parameter detection module detects the polymerization parameters, the distance between the polymerization parameter detection module, the first sub-pipeline module and the sample preparation module remains unchanged; and / or; when the polymerization parameter detection module detects the polymerization parameters, the first reduction module is kept stationary, and the sample preparation module is kept stationary in the vertical direction; and / or; when the polymerization parameter detection module detects the polymerization parameters, the polymerization parameter detection module, the first pipeline module and the sample preparation module are kept stationary.

[0012] Among them, the blood polymerization parameter detector also includes: a first joint module, the first joint module is respectively connected to the first depolymerization module and the first sub-pipeline module; the second joint module is respectively connected to the first sub-pipeline module and the second sub-pipeline module; the pipe material parameters between any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module and the second sub-pipeline module are different, and / or the pipe material parameters of the first depolymerization module and the first sub-pipeline module are different, and / or the pipe material parameters of the first sub-pipeline module and the second sub-pipeline module are different; the pipe material parameters include at least one of the inner wall roughness, material, shape and pipe diameter of the pipe.

[0013] When the module with a larger capacity in the first deaggregation module and the second deaggregation module sucks and exhales the blood sample for deaggregation, the module with a smaller capacity in the first deaggregation module and the second deaggregation module also sucks and exhales the blood sample for deaggregation.

[0014] Wherein, when the first deaggregation module and the second deaggregation module both suck out blood samples for deaggregation, the deaggregation speed of the module with smaller capacity in the first deaggregation module and the second deaggregation module is greater than the deaggregation speed of the module with larger capacity in the first deaggregation module and the second deaggregation module; and / or, the driving speed of the first deaggregation module during blood separation is less than the driving speed of the first deaggregation module during deaggregation.

[0015] The first deaggregation module and / or the second deaggregation module are used to: before the blood sample reaches the aggregation parameter detection module, the first deaggregation module and / or the second deaggregation module sucks and spits out the blood sample for deaggregation.

[0016] Among them, the capacity of the first deaggregation module is less than or equal to three times the maximum blood sample volume required for detection other than aggregation parameter detection in a single sample detection, or the capacity of the first deaggregation module is greater than the maximum optical detection sample volume required for detection other than aggregation parameter detection in a single sample detection; and / or the ratio of the capacity of the second deaggregation module to the first deaggregation module is greater than or equal to 40 and less than or equal to 120.

[0017] Among them, the blood polymerization parameter detector also includes a reaction pool module; when the sample is pushed to the reaction pool module through the sample preparation module, the first depolymerization module must perform a pushing operation, and the second depolymerization module must not perform a pushing operation, and / or, when a microliter-level non-sample liquid is pushed to the reaction pool module through the sample preparation module, the first depolymerization module must perform a pushing operation, and / or, when a microliter-level non-sample liquid is extracted through the sample preparation module, the first depolymerization module must perform an extraction operation, and / or, when a milliliter-level non-sample liquid is pushed to the reaction pool module through the sample preparation module, the second depolymerization module must perform a pushing operation, and / or, when liquid is extracted through the sample preparation module, the second depolymerization module must perform an extraction operation.

[0018] Among them, the blood polymerization parameter detector also includes at least two reaction pool modules. In the process of pushing milliliter-level non-sample liquid to the reaction pool modules through the first pipeline module and the sample preparation module in sequence, the collected milliliter-level non-sample liquid is first injected into the reaction pool module containing the hemolytic agent in at least two reaction pool modules.

[0019] Among them, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the first power; when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the second power; the first power is less than the second power.

[0020] The beneficial effects of the present application are as follows: Different from the prior art, in the technical solution of the present application, the first end of the first depolymerization module is connected to the sample preparation module, the second end of the first depolymerization module is connected to the first end of the first pipeline module, the second end of the first pipeline module is connected to the first end of the second depolymerization module, the second end of the second depolymerization module is connected to the first end of the second pipeline module, the second end of the second pipeline module is connected to the first end of the first depolymerization module, the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module, the polymerization parameter detection module is arranged on the first pipeline module or the second pipeline module, wherein, in the second depolymerization module, the first end of the second depolymerization module is connected to the first end of the second depolymerization module. When the polymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 5 and less than or equal to 50; or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 10 and less than or equal to 100. Based on the above manner, the blood sample can be first subjected to depolymerization and depolymerization treatments by each depolymerization module and each depolymerization module to reduce or eliminate the influence of the blood sample caused by relative stillness, improve the accuracy of subsequent detection of polymerization parameters by the polymerization parameter detection module, and improve the reliability of the blood polymerization parameter detector. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 This is one of the structural schematic diagrams of an embodiment of the blood polymerization parameter detector of the present application; Figure 2 This is the second structural schematic diagram of an embodiment of the blood polymerization parameter detector of the present application; Figure 3 This is a schematic diagram of the overall structure of the blood polymerization parameter detector of the present application; Figure 4 It is a structural schematic diagram of an embodiment of the second deagglomeration module of the present application.

[0023] Figure numerals: 1. sample preparation module; 2. first depolymerization module; 3. first pipeline module; 4. second depolymerization module; 41. first inlet and outlet; 42. second inlet and outlet; 5. second pipeline module; 6. first depolymerization module; 7. third pipeline module; 8. second depolymerization module; 9. polymerization parameter detection module; 10. three-way valve; 11. cleaning swab. DETAILED DESCRIPTION

[0024] The present application is further described in detail below in conjunction with the accompanying drawings and examples. It is particularly noted that the following examples are only used to illustrate the present application, but are not intended to limit the scope of the present application. Similarly, the following examples are only some embodiments of the present application rather than all embodiments, and all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present application.

[0025] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0026] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "set", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, the above-mentioned specific meanings belonging to this application can be connected according to specific circumstances.

[0027] This application proposes a blood polymerization parameter detector, see Figures 1 to 3 , Figure 1 This is one of the structural schematic diagrams of an embodiment of the blood polymerization parameter detector of the present application. Figure 2 This is the second structural diagram of an embodiment of the blood polymerization parameter detector of the present application. Figure 3 This is a schematic diagram of the overall structure of the blood polymerization parameter detector of the present application, such as Figure 1 and Figure 2 As shown, the blood polymerization parameter detector includes a sample preparation module 1, a first depolymerization module 2, a first pipeline module 3, a second depolymerization module 4, a second pipeline module 5, a first depolymerization module 6, a third pipeline module 7, a second depolymerization module 8 and a polymerization parameter detection module 9.

[0028] The sample preparation module 1 is used to prepare a blood sample to be tested, wherein the sample preparation module 1 may be an infusion needle or a sampling needle, and the sample preparation module 1 may be provided with corresponding power to inhale or spit out the blood sample.

[0029] The first end of the first deagglomeration module 2 is connected to the sample preparation module 1. The first deagglomeration module 2 can be a non-straight pipeline with a large bend, for example, Figure 1 The first deagglomeration module 2 of the U-shaped pipeline shown, for example Figure 2 The first deagglomeration module 2 of the S-shaped pipeline shown. Since the pipeline has a certain degree of roughness and the large curved section causes the flow direction of the blood sample to change rapidly, the curved pipeline affects the flow velocity and direction of the blood sample inside and the push and pull of the driving device, which can cause irregular small-scale turbulence in the local part of the pipeline, thereby breaking up, stirring, impacting, and rotating the aggregated particles, thereby achieving deagglomeration treatment.

[0030] like Figure 3 As shown, the sample preparation module 1 can be used to move along the direction of its slide rail on the track A. If the first deagglomeration module 2 connected to the upper end of the sample preparation module 1 is a U-shaped pipeline, at least part of the first pipeline module 3 connected to the first deagglomeration module 2 will extend from top to bottom, and the remaining part of the first pipeline module 3 or other pipelines connected to the first pipeline module 3 can be placed in the drag chain B, and the drag chain B can be located below the guide rail A. If the first deagglomeration module 2 connected to the upper end of the sample preparation module 1 is an S-shaped pipeline, at least part of the first pipeline module 3 connected to the first deagglomeration module 2 will extend from bottom to top, and the remaining part of the first pipeline module 3 or other pipelines connected to the first pipeline module 3 can be placed in the drag chain B, and the drag chain B can be located above the guide rail A.

[0031] The second end of the first deagglomeration module 2 is connected to the first end of the first pipeline module 3. The first deagglomeration module 2 and the first pipeline module 3 may be different parts of the same pipeline, or different pipelines made of different materials.

[0032] The second end of the first pipeline module 3 is connected to the first end of the second deagglomeration module 4. Specifically, the second deagglomeration module 4 may be a two-way valve, the inner diameter of which when turned on is different from the inner diameter of the first pipeline module 3 and the inner diameter of the second pipeline module 5, respectively. When the liquid passes through the pipeline and the two-way valve, due to the influence caused by the different inner diameters and the different local flow directions, irregular small-scale turbulence may be generated locally, thereby having the effects of breaking up, stirring, impacting, rotating, shearing, etc. on the polymerized particles, thereby achieving deagglomeration treatment.

[0033] The second end of the second deagglomeration module 4 is connected to the first end of the second pipeline module 5. The second end of the second pipeline module 5 is connected to the first end of the first deagglomeration module 6. The first deagglomeration module 6 can be connected to the second end of the second deagglomeration module 4 through the second pipeline module 5, and the first deagglomeration module 6 is used to suck and spit the liquid, so that the blood sample in the pipeline connected to the first deagglomeration module 6 moves back and forth along the extension direction of the pipeline, further enhancing the scale and strength of the irregular small-scale turbulence generated locally, and further enhancing the effects of breaking up, stirring, impacting, rotating, shearing, etc. on the aggregated particles, thereby achieving deagglomeration processing.

[0034] The second depolymerization module 8 is connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7. The second depolymerization module 8 can be connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7, so that the second depolymerization module 8 can suck and spit the liquid together with the first depolymerization module 6, so that the blood sample in the pipeline connected to it moves back and forth along the extension direction of the pipeline, further enhancing the scale and strength of the irregular small-scale turbulence generated locally, and further enhancing the effects of breaking up, stirring, impacting, rotating, and shearing the polymerized particles, thereby achieving further depolymerization processing. In addition, only one of the depolymerization modules can be used to suck and spit the liquid to achieve depolymerization processing, which can be determined according to actual needs and is not limited here.

[0035] The polymerization parameter detection module 9 is disposed on the first pipeline module 3 or the second pipeline module 5 , and is used to detect the polymerization parameters of the blood sample in the first pipeline module 3 or the second pipeline module 5 .

[0036] The aggregation parameters here may refer to the aggregation parameters of various substances in the blood, including: Proportion of Exosome Aggregation, Hemoglobin Sedimentation Ratio, Erythrocyte Sedimentation Rate, etc.

[0037] In one example, not shown in the figure, when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, that is, when the second depolymerization module 8 and the first depolymerization module 6 are based on the same pipeline as the opening and jointly perform liquid suction and discharge, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module 8 to the first depolymerization module 6 is greater than or equal to 5 and less than or equal to 50.

[0038] Or, in another example, Figure 1 and Figure 2As shown, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, that is, when the second depolymerization module 8 is connected to the first depolymerization module 6 to indirectly connect to the first pipeline module 3, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the capacity ratio of the second depolymerization module 8 to the first depolymerization module 6 is greater than or equal to 10 and less than or equal to 100.

[0039] In the above two examples, when the second depolymerization module 8 and the first depolymerization module 6 are based on the same pipeline as the opening and jointly perform liquid suction and exhalation, the second depolymerization module 8 and the first depolymerization module 6 can be made to superimpose their suction and exhalation power at their openings, so that only one of the depolymerization modules can be controlled to perform suction and exhalation when not needed, and both depolymerization modules can be controlled to perform suction and exhalation when needed; the driving control time can be carried out simultaneously or in time-sharing, and the time periods of the driving control of the two can also overlap partially, depending on the specific needs; and the direction of the driving control (suction or exhalation, push or draw) and the size of the motor driving current are superimposed to meet more depolymerization scene requirements. When the second depolymerization module 8 is connected to the first depolymerization module 6 to indirectly connect the first pipeline module 3, since the second depolymerization module 8 and the first depolymerization module 6 are in a progressive connection relationship, and since the pipe resistance of the series mode is relatively greater than the pipe resistance of the parallel mode, the value range of the ratio of the capacity of the second depolymerization module 8 to the first depolymerization module 6 can be increased, so that the two depolymerization modules in the two examples can have the ability to provide depolymerization processing with basically the same or basically similar intensity range.

[0040] In practice, the capacity of the second depolymerization module 8 is greater than that of the first depolymerization module 6. The blood sample can be collected by the sample preparation module 1 based on the first depolymerization module 6 only, and the blood sample does not pass through the second depolymerization module 4. The blood sample is collected by the depolymerization module with a smaller capacity, which can effectively improve the accuracy of sampling. When necessary, the two depolymerization modules with a smaller capacity and a larger capacity can also be controlled to collect blood samples at the same time, which can effectively improve the collection efficiency of blood samples, which is not limited here.

[0041] After completing the collection of the blood sample, the second depolymerization module 8 and the first depolymerization module 6 can be controlled to perform suction and exhalation together to depolymerize the blood sample, thereby improving the efficiency of the depolymerization process, and after the depolymerization process, the polymerization parameters of the blood sample are detected based on the polymerization parameter detection module 9, thereby reducing or eliminating the influence of different degrees of particle aggregation caused by relative stillness of blood samples for polymerization parameter detection in different batches, thereby improving the accuracy and consistency of the blood polymerization parameter detector for detecting polymerization parameters of blood samples from different batches, thereby improving the reliability of the blood polymerization parameter detector.

[0042] It should be noted that the blood polymerization parameter detector can be used to test blood routine, CRP (C-reactive protein), erythrocyte sedimentation rate (Erythrocyte Sedimentation Rate) and other parameters of blood samples. After collecting blood samples and testing the reduction, depolymerization and polymerization parameters, they can be transported to the corresponding reaction pool to obtain the test results of the corresponding parameters. In actual testing, the sample volume required for testing is usually no more than 160 microliters of blood samples, among which the sample volume required for the test of erythrocyte sedimentation rate is usually no more than 140 microliters, and the sample volume required for the test of blood routine is usually no more than 35 microliters.

[0043] The sum of the capacities of the first pipeline module 3 and the second pipeline module 5 is greater than or equal to 250 microliters, and the capacity of the sample preparation module 1 is greater than or equal to 15 microliters and less than or equal to 30 microliters, so that the first pipeline module 3, the second pipeline module 5 and the sample preparation module 1 can accommodate the blood samples required for each test respectively, and can even accommodate the total blood samples required for multiple tests at the same time.

[0044] The sum of the lengths of the first pipeline module 3 and the second pipeline module 5 may be greater than or equal to 500 mm, and the inner diameter may be less than or equal to 2 mm.

[0045] In order to obtain the reasonable parameters mentioned in our above technical solution, we borrowed the fluid mechanics model constructed in the paper Pomerenka O, Carrillo Segura S, Cao F, Wu J, Ristroph L. Hydrodynamics of finite-length pipes at intermediate Reynolds numbers [J]. Journal of Fluid Mechanics, 2023, 959: A28. to analyze the above fluid characteristics. The organizational framework of the model proposed in the paper is obtained by dimensional analysis of the smooth circular pipe flow problem. The relevant physical parameters include pipe length L and inner diameter D, fluid density ρ and viscosity μ, average flow velocity U, and pressure difference Δp. These six quantities can be regarded as one dependent variable and five independent variables, such as The specific model formula is as follows:

[0046] For the critical Reynolds number , many existing laws do not apply, and the influence of turbulent flow development has been shown to no longer be limited to a relatively short inlet region, whose length It is relatively sensitive to Re, while the above model can accurately calculate fluid friction, pressure-flow relationship and other characteristics. Among them, the six parameters and their values ​​are = 0.85, Re∗ = 2000, δ = 200, b∞ = 1, k = 0.05 and =0.04. The pipe diameter is the upper limit of the inner diameter of the first pipe module 3 or the second pipe module 5, which is 2 mm. At present, the detection speed of the whole machine of the cell parameter joint analyzer is getting faster and faster, resulting in the flow velocity in the pipe, especially the local flow velocity, which may exceed 20 m / s. Then, the Reynolds number of the fluid flow state in the pipe has a greater probability of falling into the critical interval Reynolds number. , and then substitute other empirical parameters into the above formula, and multiply the calculated sum of the lengths of the first pipeline module 3 and the second pipeline module 5 by a certain redundancy coefficient (selected as 3 based on experience), then the sum of the lengths of the first pipeline module 3 and the second pipeline module 5 is 0.4931m. After appropriate rounding, the sum of the lengths of the first pipeline module 3 and the second pipeline module 5 should be greater than or equal to 500mm. The minimum diameter of the first pipeline module 3 and the second pipeline module 5 is generally 0.8mm (if the diameter is thinner, the pipe resistance is too large, which may bring greater negative effects), then the corresponding sum of the capacities is 251.2ul, which is rounded to 250ul.

[0047] Different from the prior art, in the technical solution of the present application, the first end of the first depolymerization module 2 is connected to the sample preparation module 1, the second end of the first depolymerization module 2 is connected to the first end of the first pipeline module 3, the second end of the first pipeline module 3 is connected to the first end of the second depolymerization module 4, the second end of the second depolymerization module 4 is connected to the first end of the second pipeline module 5, the second end of the second pipeline module 5 is connected to the first end of the first depolymerization module 6, the second depolymerization module 8 is connected to the first end or the second end of the first depolymerization module 6 through the third pipeline module 7, and the polymerization parameter detection module 9 is arranged on the first pipeline module 3 or the second pipeline module 5, wherein, in the second depolymerization module 8 through When the third pipeline module 7 is connected to the first end of the first depolymerization module 6, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module 8 to the first depolymerization module 6 is greater than or equal to 5 and less than or equal to 50, or, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the sum of the capacities of the first depolymerization module 2 and the first pipeline module 3 is greater than 150 microliters, the capacity of the first depolymerization module 6 is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module 8 to the first depolymerization module 6 is greater than or equal to 10 and less than or equal to 100. The first depolymerization module 6 and the second depolymerization module 8 can be plunger pumps, which are composed of a rigid shell, a wear-resistant piston, and a driving mechanism. This type of pump relies on the contact surface between the piston and the shell to form a seal. The amount of liquid added by this type of syringe / pump is determined by the stroke of the piston movement and the inner cross-sectional area of ​​the shell on the stroke. Due to the existence of machining tolerances, the inner cross-sectional area of ​​the housing is generally different in different regions of the stroke, and generally when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the system pipe resistance driven by the second depolymerization module 8 to perform suction and exhalation is small, while when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the system pipe resistance driven by the second depolymerization module 8 to perform suction and exhalation is large. According to the model, capacity, power, driving current, static current and other parameters of the commonly used plunger pump, it can be determined that the various parameters of the first depolymerization module 6 and the second depolymerization module 8 are different.

[0048] Based on the above method, the blood sample can be first subjected to deaggregation and deaggregation treatments through the deaggregation modules and the deaggregation modules to reduce or eliminate the impact of particle aggregation caused by the relative stillness of the blood sample, thereby improving the accuracy of subsequent detection of aggregation parameters based on the aggregation parameter detection module 9 and improving the reliability of the blood aggregation parameter detector.

[0049] In one embodiment, when the first deaggregation module 6 and / or the second deaggregation module 8 aspirates the blood sample: The maximum displacement of one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module 1 .

[0050] And / or, the blood sample passes through the first pipeline module 3.

[0051] And / or, the blood sample does not pass through the second deagglomeration module 4 .

[0052] Specifically, when controlling the first deaggregation module 6 and / or the second deaggregation module 8 to aspirate or exhale the blood sample, so as to inhale or exhale the blood sample through the sample preparation module 1, the deaggregation and deaggregation of the blood sample is achieved, and in the subsequent collection and injection process, the maximum displacement of one end of the blood sample calculated from the time of entering the sample preparation module 1 is greater than 1 / 2 of the length of the inner cavity of the sample preparation module 1, which not only reduces the possibility of insufficient collected blood sample or overflowing from the sample preparation module 1 during aspiration and exhalation, but also increases the length of the pipeline through which the sample flows during aspiration and exhalation, which is equivalent to increasing the roughness of the tube wall to reduce the time and intensity of particle aggregation, thereby enhancing the deaggregation effect, thereby further improving the reliability of the blood aggregation parameter detector.

[0053] In addition, after the blood sample enters the sample preparation module 1, the blood sample passes through the first pipeline module 3 but not the second de-aggregation module 4, so that the polymerization parameters of the blood sample can be detected by the polymerization parameter detection module 9 arranged on the first pipeline module 3. Since the internal pipeline of the second de-aggregation module 4 has an irregular shape, a large variation in the tube diameter, and may have some dead corners that are difficult to clean, the possibility of the blood sample causing contamination of the second de-aggregation module 4 and making it difficult to clean can also be reduced, thereby improving the reliability of the blood polymerization parameter detector.

[0054] In one embodiment, the orientation of the opening at one end of the first deagglomeration module 2 is different from the orientation of the opening at the other end of the first deagglomeration module 2 .

[0055] And / or, the orientation of the opening at one end of the second deagglomeration module 4 is different from the orientation of the opening at the other end of the second deagglomeration module 4 .

[0056] And / or, the blood sample undergoes at least one 180-degree turn when passing through the first deagglomeration module 2 .

[0057] And / or, the blood sample undergoes at least one 180-degree turn when passing through the second deagglomeration module 4 .

[0058] And / or, the minimum pipe diameter of the second deagglomeration module 4 is different from the pipe diameter of the first pipeline module 3 .

[0059] And / or, the minimum pipe diameter of the second deagglomeration module 4 is different from the pipe diameter of the second pipeline module 5 .

[0060] And / or, the maximum pipe diameter of the second deagglomeration module 4 is different from the pipe diameter of the first pipeline module 3 .

[0061] And / or, the maximum pipe diameter of the second deagglomeration module 4 is different from the pipe diameter of the second pipeline module 5 .

[0062] And / or, the length of the first pipeline module 3 is greater than or equal to 5 cm.

[0063] Specifically, first, by making the opening direction of one end of the first deagglomeration module 2 different from the opening direction of the other end of the first deagglomeration module 2, and / or the blood sample undergoes at least one 180-degree turn when passing through the first deagglomeration module 2, the blood sample can have different directions between the first inlet and outlet and the second inlet and outlet of the first deagglomeration module 2, or undergo multiple changes in flow direction, thereby enhancing the disturbance ability of the first deagglomeration module 2 on the blood sample. By making the blood sample undergo at least one inversion process, the scale and intensity of the locally generated irregular small-scale turbulence are further enhanced, and the effects of breaking up, stirring, impacting, rotating, and shearing the aggregated particles are further enhanced, and the aggregation effect thereof is reduced by using gravity. The first deagglomeration module 2 of the U-shaped pipeline or the S-shaped pipeline as described above can achieve at least one inversion process to achieve one deagglomeration.

[0064] Similarly, see Figure 4 , Figure 4 : is a schematic diagram of the structure of an embodiment of the second deagglomeration module 4 of the present application, such as Figure 4 As shown, by making the opening direction of one end of the second deagglomeration module 4 different from the opening direction of the other end of the second deagglomeration module 4, and / or, the blood sample undergoes at least one 180-degree turn when passing through the second deagglomeration module 4, the blood sample can be made to have different directions between the first inlet and outlet 41 and the second inlet and outlet 42 of the second deagglomeration module 4, or undergo at least one change in flow direction, thereby enhancing the disturbance capability of the second deagglomeration module 4 on the blood sample, further enhancing the scale and strength of the irregular small-scale turbulence generated locally, further enhancing the effects of breaking up, stirring, impacting, rotating, and shearing the aggregated particles, and reducing the aggregation effect therein by making the blood sample undergo at least one inversion process, using gravity. Here, the minimum pipe diameter of the second deagglomeration module 4 can be the minimum diameter of the valve.

[0065] Second, by making the minimum tube diameter of the second deagglomeration module 4 different from the tube diameter of the first pipeline module 3, and / or the minimum tube diameter of the second deagglomeration module 4 is different from the tube diameter of the second pipeline module 5, and / or the maximum tube diameter of the second deagglomeration module 4 is different from the tube diameter of the first pipeline module 3, and / or the maximum tube diameter of the second deagglomeration module 4 is different from the tube diameter of the second pipeline module 5, after the blood sample passes through any of the above-mentioned two devices with different tube diameters, due to the difference in the tube diameter of the pipeline in which it flows, the scale and intensity of the locally generated irregular small-scale turbulence are further enhanced, and the effects of breaking up, stirring, impacting, rotating, and shearing the aggregated particles are further enhanced, thereby achieving further disturbance of the blood sample, thereby achieving further deagglomeration treatment.

[0066] Third, when the polymerization parameter detection module 9 is set on the first pipeline module 3, since there is a diameter reduction process of the blood sample between the first pipeline module 3 and the second deagglomeration module 4, or the second deagglomeration module 4 is a diameter reduction deagglomeration module, and there is a direction change process of the blood sample between the first pipeline module 3 and the first deagglomeration module 2, or the first deagglomeration module 2 is a direction change deagglomeration module, the scale and strength of the irregular small-scale turbulence generated locally are strengthened, and the effects of breaking up, stirring, impacting, rotating, and shearing the polymer particles are further strengthened. This method of setting the diameter reduction process and the direction change process on both sides of the first pipeline module 3 further strengthens the scale and strength of the irregular small-scale turbulence generated locally, further strengthens the effects of breaking up, stirring, impacting, rotating, and shearing the polymer particles, and can further strengthen the effect of the deagglomeration process.

[0067] Based on the difference between the maximum and minimum sedimentation rates of the blood sample and the flow rate of the blood sample, it can be determined that the first pipeline module 3 provided with the polymerization parameter detection module 9 for detecting the polymerization parameters needs to have a sufficient sedimentation distance in order to improve the accuracy of the detection of the polymerization parameters. Therefore, the reliability of the blood polymerization parameter detector can be improved by making the tube length of the first pipeline module 3 greater than or equal to 5 cm.

[0068] It should be noted that the tube length of the first pipeline module 3 is the pipeline length of the first pipeline module 3 itself, or the distance between the fixed structures (such as cable ties) located at both ends of the first pipeline module 3 for fixing the first pipeline module 3, or the pipeline length of at least part of the first pipeline module 3 wrapped by the polymerization parameter detection module 9 to prevent stray light from entering, or the pipeline length of at least part of the first pipeline module 3 wrapped by the polymerization parameter detection module 9 for heating the wrapped pipeline.

[0069] In one embodiment, the extension direction of the inner cavity of the sample preparation module 1 is parallel to the extension direction of the inner cavity of the first pipeline module 3, and the distance between the projection of the sample preparation module 1 on the horizontal plane and the projection of the first pipeline module 3 on the horizontal plane is less than 6 cm. It should be noted that all projections are a point or a circle in an ideal state, but no matter what the projection shape or pipeline shape in the three-dimensional space is, as long as there are two projections on the horizontal plane, it does not affect the limitation of the distance between the projections on the horizontal plane.

[0070] And / or, a distance between a projection of one end of the first deagglomeration module 2 on a horizontal plane and a projection of the other end of the first deagglomeration module 2 on a horizontal plane is less than 6 cm.

[0071] Specifically, the two ends of the first pipeline module 3 can be fixed on the corresponding plates. For example, the two ends of the first pipeline module 3 can be fixed by cable ties, so that the connecting line between the two ends of the first pipeline module 3 can be arranged parallel to the needle inner cavity axis of the sample preparation module 1 serving as the sampling needle. The needle inner cavity axis is the axis of the needle body inner cavity of the sample preparation module 1 excluding the needle head.

[0072] By making the distance between the projection of the sample preparation module 1 on the horizontal plane and the projection of the first pipeline module 3 on the horizontal plane less than 6 cm, or making the distance between the projection of one end of the first deagglomeration module 2 on the horizontal plane and the projection of the other end of the first deagglomeration module 2 on the horizontal plane less than 6 cm, the non-straight pipeline of the first deagglomeration module 2 sandwiched between the sample preparation module 1 and the first pipeline module 3 can have a higher degree of curvature, that is, a greater degree of curvature change, further enhancing the scale and strength of the locally generated irregular small-scale turbulence, further enhancing the effects of breaking up, stirring, impacting, rotating, and shearing the polymer particles, and improving the deagglomeration treatment effect of the first deagglomeration module 2.

[0073] In one embodiment, the first pipeline module 3 includes a first sub-pipeline module and a second sub-pipeline module that are connected to each other.

[0074] The two ends of the first sub-pipeline module are relatively fixed, so that the inner cavity axis of the first sub-pipeline module is kept as a straight line perpendicular to the horizontal plane.

[0075] One end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second deagglomeration module 4. The second sub-pipeline module can perform corresponding deformation and / or movement when one end of the second sub-pipeline module and the other end of the second sub-pipeline module move relative to each other.

[0076] Specifically, the two ends of the first sub-pipeline module can be fixed on the corresponding plates. For example, the two ends of the first sub-pipeline module can be fixed by cable ties, so that the inner cavity axis of the pipeline part between the two ends of the first sub-pipeline module is a straight line perpendicular to the horizontal plane, and one end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, thereby realizing the fixation of this section of the second sub-pipeline module, and the other unfixed end of the second sub-pipeline module can pass through the drag chain B mentioned above and be connected to the second deagglomeration module 4. Based on the above method, by fixing and dragging the chain B, the pipeline shaking of the blood sample during the deagglomeration and disagglomeration treatment can be reduced, thereby reducing the inconsistency between blood samples caused by turbulence caused by pipeline shaking during the deagglomeration and disagglomeration treatment, improving the consistency of detection of blood samples from different batches, and thereby improving the reliability of the blood polymerization parameter detector.

[0077] Optionally, when the polymerization parameter detection module 9 detects the polymerization parameter, the distance between the polymerization parameter detection module 9, the first sub-pipeline module and the sample preparation module 1 remains unchanged.

[0078] And / or, when the polymerization parameter detection module 9 detects the polymerization parameter, the first depolymerization module 2 is kept stationary, and the sample preparation module 1 is kept stationary in the vertical direction.

[0079] And / or, when the polymerization parameter detection module 9 detects the polymerization parameter, the polymerization parameter detection module 9, the first pipeline module 3, and the sample preparation module 1 are kept stationary.

[0080] Specifically, when the polymerization parameter detection module detects the polymerization parameters, the distance between the polymerization parameter detection module 9, the first sub-pipeline module and the sample preparation module 1 remains unchanged, and / or the first reduction module 2 is kept stationary, and the sample preparation module 1 is kept stationary in the vertical direction, and / or the polymerization parameter detection module 9, the first pipeline module 3 and the sample preparation module 1 are kept stationary. This can reduce the blood sedimentation of the blood sample in the first pipeline module 3 detected by the polymerization parameter detection module 9, that is, by making the first sub-pipeline module itself sufficiently stable, the accuracy of the polymerization parameter detection module 9 during detection can be improved.

[0081] Furthermore, the blood polymerization parameter detector also includes a first connector module and a second connector module.

[0082] The first connector module is respectively connected to the first deagglomeration module 2 and the first sub-pipeline module.

[0083] The second connector module is respectively connected to the first sub-pipeline module and the second sub-pipeline module.

[0084] The pipe material parameters between any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module and the second sub-pipeline module are different, and / or the pipe material parameters of the first deagglomeration module 2 and the first sub-pipeline module are different, and / or the pipe material parameters of the first sub-pipeline module and the second sub-pipeline module are different, and the pipe material parameters include at least one of the roughness of the inner wall, material, shape and pipe diameter.

[0085] Specifically, the first sub-pipeline module can be a glass tube, for example, a square outer diameter glass tube. The polymerization parameter detection module 9 can be arranged on the outside of the glass tube serving as the first sub-pipeline module, and the polymerization parameters of the blood sample therein are detected through the glass tube. The first joint module and the second joint module respectively adjacent to the first sub-pipeline module can be pipelines of other material types other than glass tubes, which are not limited here.

[0086] Furthermore, the first sub-pipeline module can be a glass tube with a square outer diameter and a circular inner diameter, and the first joint module and the second joint module adjacent to the first sub-pipeline module can be pipes of other material types other than glass tubes, and the inner diameters of the first joint module and the second joint module can be completely the same as those of the first sub-pipeline module, and the connecting joint has a smooth transition, which reduces the possibility of local turbulence due to the uneven shape of the transition or the change in the pipe diameter at the connecting joint, thereby improving the accuracy of the polymerization parameter detection module 9 in detecting the polymerization parameters of the blood sample. Similarly, the second sub-pipeline module can also be a glass tube, for example, a glass tube with a square outer diameter and a circular inner diameter, and the polymerization parameter detection module 9 can be set on the outside of the glass tube serving as the second sub-pipeline module, and the polymerization parameters of the blood sample therein can be detected through the glass tube, and the second joint module adjacent to the second sub-pipeline module can be a pipe of other material types other than glass tubes, which is not limited here.

[0087] Optionally, the pipeline material of the first sub-pipeline module is at least one of polyetheretherketone, polytetrafluoroethylene, polyethylene and polypropylene, which can enable the light beam generated by the polymerization parameter detection module 9 to enter the first sub-pipeline module to detect the cell vertical parameters of the sample, thereby improving the measurement accuracy of the polymerization parameter detection module 9.

[0088] In one embodiment, the transmittance of the first sub-pipeline module is greater than or equal to 90%, and the haze is less than or equal to 5%, so that almost all of the light beams emitted by the polymerization parameter detection module 9 enter the first sub-pipeline module for detection, thereby improving the measurement accuracy of the polymerization parameter detection module 9.

[0089] Based on the aforementioned method of arranging a variable diameter deagglomeration module and a variable direction deagglomeration module on both sides of the first pipeline module 3, the scale and strength of the irregular small-scale turbulence generated locally are further enhanced, and the effects of breaking up, stirring, impacting, rotating, and shearing the aggregate particles are further enhanced, which can further enhance the effect of the deagglomeration treatment.

[0090] At the same time, it is ensured that the detection part of the polymerization parameter detection module 9 uses a more transparent and easier-to-clean material, thereby ensuring the accuracy of the polymerization parameter detection of the blood sample. When the blood sample is transported in the glass tube, the inner wall of the glass tube has a lower roughness, a more stable material, a more flat shape, and a more suitable tube diameter, which can effectively improve the stability of the blood sample when it is transported in the glass tube, thereby improving the accuracy of the polymerization parameter detection module 9 in detecting the polymerization parameters of the blood sample through the glass tube.

[0091] In addition, the first connector module and the second connector module may be provided with chamfers at the connection, which can reduce the possibility of blood sample remaining at the connection.

[0092] In one embodiment, when a module with a larger capacity in the first deaggregation module 6 and the second deaggregation module 8 aspirates blood samples for deaggregation, a module with a smaller capacity in the first deaggregation module 6 and the second deaggregation module 8 also aspirates blood samples for deaggregation.

[0093] Specifically, deaggregation can be performed based on the first deaggregation module 6 alone or based on the first deaggregation module 6 and the second deaggregation module 8 at the same time. When a module with a larger capacity is required for deaggregation, a larger deaggregation force is usually required. Therefore, the first deaggregation module 6 and the second deaggregation module 8 can be controlled to jointly aspirate the exhaled blood sample for deaggregation to improve the deaggregation efficiency. When a deaggregation module with a smaller capacity is used for deaggregation, a smaller deaggregation force may be required. Therefore, only the one deaggregation module can be controlled for deaggregation, or both deaggregation modules can be controlled for deaggregation, depending on the specific needs.

[0094] Based on the above method, the rationality of the depolymerization intensity when controlling the depolymerization module for depolymerization can be improved, thereby improving the rationality and reliability of depolymerization.

[0095] Optionally, when both the first deaggregation module 6 and the second deaggregation module 8 aspirate and spit out blood samples for deaggregation, the deaggregation speed of the module with a smaller capacity in the first deaggregation module 6 and the second deaggregation module 8 is greater than the deaggregation speed of the module with a larger capacity in the first deaggregation module 6 and the second deaggregation module 8; And / or, the driving speed of the first deaggregation module 6 during blood separation is lower than the driving speed of the first deaggregation module 6 during deaggregation.

[0096] Specifically, the depolymerization speed may be the volume of liquid delivered per unit time by the corresponding depolymerization module when performing an extraction operation or a pushing operation. For example, when the depolymerization module is a syringe, the depolymerization speed may also be understood as the product value between the number of syringe push and pull steps of the corresponding syringe per unit time and the volume corresponding to the number of syringe single steps.

[0097] When the first deaggregation module 6 and the second deaggregation module 8 both suck and exhale blood samples for deaggregation, the deaggregation speed of the module with smaller capacity in the first deaggregation module 6 and the second deaggregation module 8 is greater than the deaggregation speed of the module with larger capacity in the first deaggregation module 6 and the second deaggregation module 8, so that the amount of extraction or pushing operation in unit time of the first deaggregation module 6 and the second deaggregation module 8 can be relatively similar, thereby achieving stability when the blood samples are deaggregated synchronously, thereby improving the reliability of deaggregation.

[0098] By making the driving speed of the first deaggregation module 6 during blood separation lower than the driving speed of the first deaggregation module 6 during deaggregation, the accuracy during blood separation can be relatively high, while the efficiency during deaggregation can be relatively high. Blood separation pays more attention to accuracy, while deaggregation pays more attention to efficiency. Based on this method, the rationality of the deaggregation module control during blood separation and deaggregation can be improved simultaneously, thereby improving the reliability during blood separation and deaggregation.

[0099] In one embodiment, the first deaggregation module 6 and / or the second deaggregation module 8 are used to: before the blood sample reaches the aggregation parameter detection module 9, the first deaggregation module 6 and / or the second deaggregation module 8 aspirates the blood sample for deaggregation.

[0100] Specifically, the blood sample can be depolymerized before it reaches the polymerization parameter detection module 9, so that the blood sample located between the pipeline where the polymerization parameter detection module 9 is located and the blood sample inlet end of the sample preparation module 1 is depolymerized by the first depolymerization module 6 and / or the second depolymerization module 8, and then the polymerization parameter detection module 9 completes the detection of the polymerization parameters of the depolymerized blood sample, thereby improving the accuracy of the detection.

[0101] In one embodiment, if Figure 1 As shown, the blood polymerization parameter detector includes a three-way valve 10.

[0102] The common end of the three-way valve 10 is connected to the second depolymerization module 8 , the first branch port of the three-way valve 10 is connected to the first depolymerization module 6 , and the second branch port of the three-way valve 10 is connected to a container for storing the diluent.

[0103] Specifically, when the liquid in the pipeline is insufficient but depolymerization is required, the second depolymerization module 8 can be controlled to draw diluent from the container storing the diluent to fill the drawn diluent into the pipeline with insufficient liquid, thereby ensuring the smooth progress of the depolymerization process. The pipeline filled with diluent, combined with the suction and exhalation of the second depolymerization module 8, can provide power for the depolymerization process for the blood sample, thereby improving the reliability of the blood polymerization parameter detector.

[0104] In one embodiment, the capacity of the first depolymerization module 6 is less than or equal to three times the maximum blood sample volume required for detection other than polymerization parameter detection in a single sample detection; for example, if the maximum blood sample volume required for detection other than polymerization parameter detection in a single sample detection is 45ul, then the capacity of the first depolymerization module 6 may be 100ul. As mentioned above, the smaller the capacity of the depolymerization module, the higher the precision of aspiration and exhalation will generally be, thereby improving the accuracy of the measurement of the entire machine.

[0105] Specifically, in one example, the amount of blood sample required for testing other than polymerization parameter testing in a single sample test may generally be 40 microliters, of which 35 microliters may be used for routine blood testing, and the capacity of the first depolymerization module 6 is less than or equal to 120 microliters. The first depolymerization module 6 in this example may be used to perform sample splitting operations on the collected blood sample. In addition, the amount of blood sample required for polymerization parameter testing is generally 140 microliters, and a blood sample of the sum of 140 microliters and 40 microliters is usually sucked in, and a total of 20 microliters of blood sample at the head and tail ends are discarded, and a 160 microliter blood sample is used to perform polymerization parameter testing, as well as sample splitting operations for routine blood testing or other tests.

[0106] In another example, the capacity of the first depolymerization module 6 may be less than or equal to 250 μL, for example, 250 μL. The first depolymerization module 6 in this example may be used to perform sample separation on the collected blood sample and as a driving source for flow-type sheath fluid auxiliary propulsion, which is not limited here.

[0107] Specifically, in one embodiment, the blood polymerization parameter detector further includes a reaction pool module, and the capacity of the first depolymerization module 6 is greater than the maximum optical detection sample volume required for detection other than polymerization parameter detection in a single sample detection. The optical detection sample volume here is the amount of liquid after the sample and reagent are added to the reaction pool module and reacted, and after being pushed and pulled, it is sent to the optical detection module (such as an optical flow chamber) for measurement. For example, if the maximum optical detection sample volume required for detection other than polymerization parameter detection in a single sample detection is 215ul, the capacity of the first depolymerization module 6 can be 250ul. Since the first depolymerization module 6 performs other tasks in addition to depolymerization, it also completes other tasks well while ensuring a high degree of suction and discharge accuracy. Moreover, when the optical detection module (such as the optical flow chamber) performs measurement, the liquid circuit stability is extremely required. When the first depolymerization module is pushed in a single unidirectional manner, the corresponding liquid circuit stability can be guaranteed. However, if a withdrawal process is involved in the middle, the return difference of its components is sufficient to destroy the stability of the liquid circuit. Therefore, the optical detection module (such as the optical flow chamber) must perform measurement by a single unidirectional push. Therefore, the capacity of 6 is greater than the maximum optical detection sample volume required for detection other than polymerization parameter detection in a single sample detection.

[0108] In another embodiment, the capacity ratio of the second deaggregation module 8 to the first deaggregation module 6 is greater than or equal to 40 and less than or equal to 120. For example, if the capacity of the first deaggregation module 6 is 100ul or 250ul, and the capacity of the second deaggregation module 8 is 10ml, then the capacity ratio is 100 times or 40 times, respectively. The second deaggregation module 8 is used to perform an extraction operation to collect a blood sample through the sample preparation module 1, and the first deaggregation module 6 is used to perform a pushing operation to sample the blood sample through the sample preparation module 1.

[0109] The second deaggregation module 8 is used for performing an extraction operation so as to collect the blood sample through the sample preparation module 1. Since the capacity of the second deaggregation module 8 is relatively large, the efficiency of the collection operation can be effectively improved.

[0110] The first deaggregation module 6 is used for performing a pushing operation so as to perform a sample separation operation on the blood sample through the sample preparation module 1. Since the capacity of the first deaggregation module 6 is relatively small, the accuracy of the sample separation operation can be effectively improved.

[0111] In one embodiment, the blood polymerization parameter detector further includes a reaction pool module, in which a blood sample and a related reaction reagent can be contained and reacted to obtain a reacted sample, wherein part of the reacted sample can be sent to an optical detection module (such as an optical flow chamber) for measurement. Then the optical detection sample volume is the liquid after the sample and the reaction reagent are added to the reaction pool module and reacted, and after being pushed and pulled, the liquid volume sent to the optical detection module (such as an optical flow chamber) for measurement. The optical detection sample volume is less than the sample volume of the reacted sample, and the reacted sample that is not sent to the optical detection module (such as an optical flow chamber) for measurement can be discharged from the waste liquid pool as waste liquid. In one embodiment, when the sample is pushed to the reaction pool module by the sample preparation module 1, the first depolymerization module 6 must perform a pushing operation, and the second depolymerization module 8 must not perform a pushing operation; the smaller the capacity of the depolymerization module, the higher the precision of the suction and discharge, thereby improving the accuracy of the whole machine measurement. Since the capacity of the second depolymerization module 8 is much larger than the capacity of the first depolymerization module 6, the first depolymerization module 6 distributes the sample more accurately, thereby improving the accuracy of the whole machine measurement.

[0112] In one embodiment, when the microliter non-sample liquid is pushed to the reaction pool module through the sample preparation module 1, the first depolymerization module 6 must perform the pushing operation; the microliter non-sample liquid here can be the dye solution in the reaction reagent, and the amount is generally only about 20ul, and the maximum does not exceed 100ul, and the price is expensive. Because the capacity of the second depolymerization module 8 is much larger than the capacity of the first depolymerization module 6, the first depolymerization module 6 distributes the microliter non-sample liquid more accurately, which not only reduces the waste caused by the inaccuracy of the microliter non-sample liquid, but also improves the accuracy of the whole machine measurement.

[0113] In one embodiment, when extracting microliter non-sample liquid through the sample preparation module 1, the first depolymerization module 6 must perform the extraction operation; because the capacity of the second depolymerization module 8 is much larger than the capacity of the first depolymerization module 6, the first depolymerization module 6 can extract the microliter non-sample liquid more accurately, which not only reduces the waste caused by the inaccurate microliter non-sample liquid, but also improves the accuracy of the whole machine measurement.

[0114] In one embodiment, when the milliliter-level non-sample liquid is pushed to the reaction pool module through the sample preparation module 1, the second depolymerization module 8 must perform a pushing operation; since the capacity of the second depolymerization module 8 is much larger than that of the first depolymerization module 6, the driving and pushing capability is stronger, the cleaning efficiency is higher, and the corrosion to the pipeline is reduced. The milliliter-level non-sample liquid here can be a probe liquid sucked inside or outside the machine, and the amount generally exceeds 100ul. When using the probe liquid, the amount usually required is relatively large, and more places need to be cleaned. For example, wherever a blood sample passes, there may be residual protein in the blood, and the probe liquid needs to be cleaned.

[0115] In one embodiment, when liquid is extracted through the sample preparation module 1, the second depolymerization module 8 must perform the extraction operation; since the capacity of the second depolymerization module 8 is much larger than the capacity of the first depolymerization module 6, the driving suction capacity is stronger and the extraction efficiency is higher. The liquid here includes the sample (liquid) and the milliliter-level non-sample liquid. When the milliliter-level non-sample liquid is extracted, the extraction speed is fast, which reduces the retention time of the milliliter-level non-sample liquid in the pipeline, thereby reducing the corrosion to the pipeline; when extracting the sample, since the capacity of the second depolymerization module 8 is much larger than the capacity of the first depolymerization module 6, the driving suction capacity of the sample is stronger, the sample can be collected faster, and the speed and efficiency of the whole machine are accelerated. Specifically, in one example, the blood polymerization parameter detector also includes at least two reaction pool modules, wherein, in the process of pushing the milliliter-level non-sample liquid to the reaction pool module through the first pipeline module 3 and the sample preparation module 1 in turn, the collected milliliter-level non-sample liquid is first injected into the reaction pool module containing the hemolytic agent in at least two reaction pool modules.

[0116] In this example, before the collected milliliter-level non-sample liquid is injected into all the reaction pool modules, the reaction pool modules containing hemolytic agents among all the reaction pool modules may be determined first. Since the hemolytic agent will dissolve the protein in the blood sample, the protein is easy to adhere to the pipeline components, and the amount of blood sample in the reaction pool module containing the hemolytic agent is usually relatively large, the collected milliliter-level non-sample liquid may be first injected into the reaction pool module containing the hemolytic agent among all the reaction pool modules, and then the collected milliliter-level non-sample liquid may be injected into the reaction pool module not containing the hemolytic agent among all the reaction pool modules, so that the reaction pool modules containing the hemolytic agent can have more milliliter-level non-sample liquid immersion time relative to the reaction pool modules not containing the hemolytic agent, thereby enhancing the cleaning effect of the reaction pool modules containing the hemolytic agent.

[0117] Optionally, when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, the driving power of the second depolymerization module 8 during the extraction operation or the pushing operation is the first power; When the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the driving power of the second depolymerization module 8 during the extraction operation or the pushing operation is the second power; The first power is less than the second power.

[0118] Specifically, when the second depolymerization module 8 is connected to the second end of the first depolymerization module 6 through the third pipeline module 7, the second depolymerization module 8 and the sample preparation module 1 are indirectly connected, with the first depolymerization module 6 in between, relative to when the second depolymerization module 8 is connected to the first end of the first depolymerization module 6 through the third pipeline module 7, and therefore, the second depolymerization module 8 has a greater resistance when performing extraction or pushing operations. Based on the above method, by making the first power smaller than the second power, it can be ensured that in both cases, the ability of the second depolymerization module 8 to drive the liquid is strong enough to ensure the smooth progress of the extraction or pushing operations, thereby improving the reliability of the blood polymerization parameter detector.

[0119] In one embodiment, if Figure 1 and Figure 2 As shown, the blood polymerization parameter detector may further include a cleaning swab 11 , and the cleaning swab 11 is used to be sleeved on the sample preparation module 1 to clean the outer wall of the sample preparation module 1 .

[0120] Specifically, in practice, the cleaning swab 11 may have a liquid inlet and a liquid outlet. The liquid inlet may be connected to the second depolymerization module 8 through a corresponding two-way valve, and the liquid outlet may be connected to a waste liquid pool. For example, the inhaled diluent may be input through the liquid inlet through the second depolymerization module 8 so that the diluent cleans the outer wall of the sample preparation module 1, and then the cleaned waste liquid is output through the liquid outlet to be discharged into the waste liquid pool or other types of waste liquid channels to complete the cleaning.

[0121] Based on the above manner, since the capacity of the second deagglomeration module 8 is relatively large, the cleaning effect of the cleaning swab 11 can be improved, and the cleaning efficiency can be improved.

[0122] In the description of the present application, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradicting each other.

[0123] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0124] Any process or method description in a flowchart or otherwise described herein may be understood to represent a module, segment or portion of code that includes one or more executable instructions for implementing the steps of a specific logical function or process, and the scope of the preferred embodiments of the present application includes alternative implementations in which functions may not be performed in the order shown or discussed, including performing functions in a substantially simultaneous manner or in the reverse order depending on the functions involved, which should be understood by technicians in the technical field to which the embodiments of the present application belong.

[0125] The logic and / or steps represented in the flowchart or otherwise described herein, for example, can be considered as an ordered list of executable instructions for implementing the logical functions, and can be embodied in any computer-readable medium for use by an instruction execution system, device or apparatus (which can be a personal computer, server, network device or other system that can fetch instructions from the instruction execution system, device or apparatus and execute the instructions), or in combination with these instruction execution systems, devices or apparatuses. For the purpose of this specification, "computer-readable medium" can be any device that can contain, store, communicate, propagate or transmit a program for use by an instruction execution system, device or apparatus or in combination with these instruction execution systems, devices or apparatuses. More specific examples of computer-readable media (a non-exhaustive list) include the following: an electrical connection with one or more wires (electronic device), a portable computer disk box (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable and programmable read-only memory (EPROM or flash memory), a fiber optic device, and a portable compact disk read-only memory (CDROM). In addition, the computer-readable medium may even be paper or other suitable medium on which the program is printed, since the program may be obtained electronically, for example, by optically scanning the paper or other medium and then editing, interpreting or processing in other suitable ways if necessary, and then stored in a computer memory.

[0126] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A blood polymerization parameter detector, characterized in that: include: A sample preparation module, the sample preparation module is used to prepare a blood sample to be tested; a first aggregation reduction module, wherein a first end of the first aggregation reduction module is connected to the sample preparation module; a first pipeline module, wherein the second end of the first reduction module is connected to the first end of the first pipeline module; a second aggregation reduction module, wherein the second end of the first pipeline module is connected to the first end of the second aggregation reduction module; a second pipeline module, wherein the second end of the second reduction module is connected to the first end of the second pipeline module; a first depolymerization module, wherein the second end of the second pipeline module is connected to the first end of the first depolymerization module; The third pipeline module; a second depolymerization module, wherein the second depolymerization module is connected to the first end or the second end of the first depolymerization module through the third pipeline module; A polymerization parameter detection module, the polymerization parameter detection module is arranged on the first pipeline module or the second pipeline module, and the polymerization parameter detection module is used to detect the polymerization parameter of the blood sample in the first pipeline module or the second pipeline module; Wherein, when the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the capacity of the first depolymerization module is greater than or equal to 5 and less than or equal to 50, Or, when the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the sum of the capacities of the first depolymerization module and the first pipeline module is greater than 150 microliters, the capacity of the first depolymerization module is greater than 80 microliters, and the ratio of the capacity of the second depolymerization module to the first depolymerization module is greater than or equal to 10 and less than or equal to 100.

2. The blood polymerization parameter detector according to claim 1, characterized in that: When the first deaggregation module and / or the second deaggregation module aspirates the blood sample, The maximum displacement of one end of the blood sample is greater than 1 / 2 of the length of the inner cavity of the sample preparation module, and / or, the blood sample passes through the first pipeline module, And / or, the blood sample does not pass through the second deagglomeration module.

3. The blood polymerization parameter detector according to claim 1, characterized in that: The orientation of the opening at one end of the first deagglomeration module is different from the orientation of the opening at the other end of the first deagglomeration module; and / or, the orientation of the opening at one end of the second aggregation reduction module is different from the orientation of the opening at the other end of the second aggregation reduction module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the first deagglomeration module; and / or, the blood sample undergoes at least one 180-degree turn when passing through the second deagglomeration module; and / or, the minimum pipe diameter of the second deagglomeration module is different from the pipe diameter of the first pipeline module; and / or, the minimum pipe diameter of the second deagglomeration module is different from the pipe diameter of the second pipeline module; and / or, the maximum pipe diameter of the second deagglomeration module is different from the pipe diameter of the first pipeline module; and / or, the maximum pipe diameter of the second deagglomeration module is different from the pipe diameter of the second pipeline module; And / or, the length of the first pipeline module is greater than or equal to 5 cm.

4. The blood polymerization parameter detector according to claim 1, characterized in that: The extension direction of the inner cavity of the sample preparation module is parallel to the extension direction of the inner cavity of the first pipeline module, and the distance between the projection of the sample preparation module on the horizontal plane and the projection of the first pipeline module on the horizontal plane is less than 6 cm; And / or, a distance between a projection of one end of the first deagglomeration module on the horizontal plane and a projection of the other end of the first deagglomeration module on the horizontal plane is less than 6 cm.

5. The blood polymerization parameter detector according to claim 1, characterized in that: The first pipeline module includes a first sub-pipeline module and a second sub-pipeline module connected to each other; The two ends of the first sub-pipeline module are relatively fixed, so that the inner cavity axis of the first sub-pipeline module is kept as a straight line perpendicular to the horizontal plane; One end of the second sub-pipeline module is connected to one end of the first sub-pipeline module, and the other end of the second sub-pipeline module is connected to the second deagglomeration module. The second sub-pipeline module can perform corresponding deformation and / or movement when one end of the second sub-pipeline module and the other end of the second sub-pipeline module move relative to each other.

6. The blood polymerization parameter detector according to claim 5, characterized in that: When the aggregation parameter detection module detects the aggregation parameter, the distances among the aggregation parameter detection module, the first sub-pipeline module, and the sample preparation module remain unchanged; and / or; when the aggregation parameter detection module detects the aggregation parameter, keeping the first aggregation reduction module stationary and keeping the sample preparation module stationary in the vertical direction; and / or; when the polymerization parameter detection module detects the polymerization parameter, keeping the polymerization parameter detection module, the first pipeline module, and the sample preparation module stationary.

7. The blood polymerization parameter detector according to claim 6, characterized in that: The blood polymerization parameter detector also includes: a first connector module, the first connector module being respectively connected to the first deagglomeration module and the first sub-pipeline module; a second connector module, the second connector module being respectively connected to the first sub-pipeline module and the second sub-pipeline module; The pipe material parameters of any two adjacent modules among the first joint module, the first sub-pipeline module, the second joint module and the second sub-pipeline module are different. and / or, the first deagglomeration module and the first sub-pipeline module have different pipe material parameters, and / or, the first sub-pipeline module and the second sub-pipeline module have different pipe material parameters; The pipe material parameters include at least one of the pipe inner wall roughness, material, shape and pipe diameter.

8. The blood polymerization parameter detector according to claim 1, characterized in that: When the module with a larger capacity among the first deaggregation module and the second deaggregation module sucks and discharges the blood sample for deaggregation, the module with a smaller capacity among the first deaggregation module and the second deaggregation module also sucks and discharges the blood sample for deaggregation.

9. The blood polymerization parameter detector according to claim 8, characterized in that: When both the first deaggregation module and the second deaggregation module suck and discharge the blood sample for deaggregation, the deaggregation speed of the module with a smaller capacity among the first deaggregation module and the second deaggregation module is greater than the deaggregation speed of the module with a larger capacity among the first deaggregation module and the second deaggregation module; And / or, the driving speed of the first deaggregation module during blood separation is lower than the driving speed of the first deaggregation module during deaggregation.

10. The blood polymerization parameter detector according to claim 1, characterized in that: The first deaggregation module and / or the second deaggregation module are used for: Before the blood sample reaches the aggregation parameter detection module, the first deaggregation module and / or the second deaggregation module aspirates and exhales the blood sample for deaggregation.

11. The blood polymerization parameter detector according to claim 1, characterized in that: The capacity of the first depolymerization module is less than or equal to three times the maximum blood sample volume required for testing other than polymerization parameter testing in a single sample test. Or, the capacity of the first depolymerization module is greater than the maximum optical detection sample volume required for detection other than polymerization parameter detection in a single sample detection; And / or, a capacity ratio of the second deaggregation module to the first deaggregation module is greater than or equal to 40 and less than or equal to 120.

12. The blood polymerization parameter detector according to claim 1, characterized in that: The blood polymerization parameter detector also includes a reaction pool module; When the sample preparation module pushes the sample to the reaction pool module, the first depolymerization module must perform the pushing operation, and the second depolymerization module must not perform the pushing operation. and / or, when the sample preparation module pushes a microliter non-sample liquid to the reaction pool module, the first depolymerization module must perform a pushing operation, and / or, when the sample preparation module extracts microliter non-sample liquid, the first depolymerization module must perform an extraction operation, and / or, when the sample preparation module pushes milliliter-level non-sample liquid to the reaction pool module, the second depolymerization module must perform a pushing operation, And / or, when liquid is extracted by the sample preparation module, the second depolymerization module must perform an extraction operation.

13. The blood polymerization parameter detector according to claim 1, characterized in that: The blood polymerization parameter detector also includes at least two reaction pool modules. In the process of pushing the milliliter-level non-sample liquid to the reaction pool module through the first pipeline module and the sample preparation module in sequence, First, the collected milliliter-level non-sample liquid is injected into the reaction pool module containing the hemolytic agent among the at least two reaction pool modules.

14. The blood polymerization parameter detector according to claim 1, characterized in that: When the second depolymerization module is connected to the first end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the first power; When the second depolymerization module is connected to the second end of the first depolymerization module through the third pipeline module, the driving power of the second depolymerization module during the extraction operation or the pushing operation is the second power; The first power is less than the second power.

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