QuEChERS treatment and detection device for detecting antibiotics in aquatic products

Abstract

The invention provides a QuEChERS treatment and detection device for detecting antibiotics in aquatic products, belongs to the technical field of aquatic product detection, and solves the problems of high cost, low efficiency and the like in the existing detection and treatment of the antibiotics in the aquatic products. Comprising a clamping outer box, two transverse moving assemblies are arranged above the clamping outer box, longitudinal moving assemblies are arranged on the transverse moving assemblies, a plurality of discharging vertical moving assemblies are arranged on the side portion of one longitudinal moving assembly, powder quantifying mechanisms are arranged on the discharging vertical moving assemblies, a switching vertical moving assembly is arranged on one side of the other longitudinal moving assembly, and the powder quantifying mechanisms are arranged on the switching vertical moving assembly. A switching clamping assembly is arranged at the lower end of the switching vertical moving assembly, a plurality of liquid injection vertical moving assemblies are arranged on the other side of the other longitudinal moving assembly, liquid drainage injection mechanisms are arranged on the liquid injection vertical moving assemblies, a plurality of clamping arm assemblies are arranged on the middle pipe frame assembly, and an oscillation mechanism, a centrifugal mechanism, a position adjusting nitrogen blowing mechanism and a temperature adjusting water bath device are arranged in the clamping outer box. According to the invention, low-cost and high-efficiency batch antibiotic treatment detection can be carried out.

Description

Technical Field

[0001] The invention belongs to the technical field of aquatic product detection, and relates to an antibiotic detection device, in particular to a QuEChERS processing detection device for antibiotic detection in aquatic products. Background Art

[0002] Antibiotics are organic substances produced by organisms (including microorganisms, plants and animals) in their life activities (or obtained by other methods) that can selectively inhibit or kill other biological functions at low concentrations. Penicillin, streptomycin, erythromycin, etc. are all well-known antibiotics.

[0003] Antibiotics can inhibit or kill certain microorganisms at low concentrations. Today, most aquatic animal diseases are caused by infection with different pathogenic bacteria, which can be killed by antibiotics. For example, pathogenic Aeromonas produced in aquaculture can be inhibited by chloramphenicol. Others promote the growth of aquatic animals. Some types of antibiotics have the effect of promoting the growth of aquatic animals.

[0004] Antibiotic drugs will remain in aquatic products. Most antibiotics will be excreted in large quantities by aquatic organisms after use, and only a very small amount will remain in the body. Hao Qinwei and others studied the content of various antibiotics in fish and found that the accumulation amount in different fish tissues varies with the type of antibiotics used. Further research data shows that the ability of antibiotics to accumulate in fish blood, muscle and liver is stronger than that in other tissues. The muscle tissue in the fish body is the most consumed part by consumers. Long-term consumption will cause the ingested trace amounts of antibiotics to gradually accumulate in the human body, which may eventually cause human health problems.

[0005] Antibiotic residues directly affect food safety, thus negatively affecting the marketability of aquatic products. In addition, wild fish also ingest antibiotic residues, thus affecting the safety of captured fisheries. Excessive intake of antibiotic residues by humans can cause adverse drug reactions and drug resistance in clinically important pathogens.

[0006] Therefore, it is necessary to analyze and detect antibiotics in aquatic products. The existing analytical methods for antibiotics include chromatography and its mass spectrometry, enzyme immunoassay, and capillary electrophoresis. The enzyme immunoassay method has high selectivity for reagents and it is difficult to analyze multiple components at the same time. There is a certain degree of cross-reactivity for compounds with similar structures, and there are certain difficulties in analyzing compounds with very small molecular weight and unstable compounds.

[0007] After searching, for example, Chinese patent documents disclose a detection pretreatment device based on QuEChERS purification technology [Application No.: 202021492018.5; Publication No.: CN212621813U]. This device includes a pretreatment stirring tank, a force guide column is welded at the bottom of the pretreatment stirring tank, an amplitude frame is fixed at the bottom of the force guide column, a mounting base is slidably connected to the bottom of the amplitude frame, and both sides of the mounting base are fixed with limit clamps, and a positioning column sleeve is fixed to one side of the limit clamp by screws, and a spring is assembled inside the positioning column sleeve. The processing equipment pushes the conduction limit rod 18 to squeeze the spring 17 through the contact between the two sides of the amplitude frame 19 and the conduction limit rod 18, and uses the elastic expansion force support of the spring 17 to avoid left and right force overtravel during shaking, and transmits the amplitude to the pretreatment stirring tank 1 through the guide column 24, driving the detection mixed solution inside the pretreatment stirring tank 1 to perform stable centrifugal oscillation. Although it meets the detection standards, improves the use effect of the device, and reduces the manpower consumption during use, the processing function is single, and can only be oscillated, and the centrifugal effect is poor. Moreover, through the design of the self-cleaning structure, although it can be conveniently automatically flushed to avoid affecting the accuracy of the next detection, it needs to be automatically flushed before continuing the detection, which is inefficient and has a high detection cost.

[0008] Based on this, we proposed a QuEChERS processing and detection device for antibiotic detection in aquatic products, which can perform low-cost and high-efficiency batch antibiotic processing and detection. Summary of the invention

[0009] The purpose of the present invention is to address the above-mentioned problems in the existing technology and to propose a QuEChERS processing detection device for detecting antibiotics in aquatic products. The technical problem to be solved by the invention is: how to achieve low-cost, high-efficiency and batch antibiotic processing detection.

[0010] The purpose of the present invention can be achieved by the following technical solutions:

[0011] A QuEChERS processing detection device for detecting antibiotics in aquatic products comprises a clamping outer box, two transverse movement components are arranged above the clamping outer box, longitudinal movement components are arranged on the transverse movement components, a plurality of material discharging vertical movement components are arranged on the side of one of the longitudinal movement components, a powder quantitative mechanism is arranged on the material discharging vertical movement components, a switching vertical movement component is arranged on one side of the other longitudinal movement component, a switching clamping component is arranged at the lower end of the switching vertical movement component, a plurality of liquid injection vertical movement components are arranged on the other side of the other longitudinal movement component, a liquid extraction and discharge liquid injection mechanism is arranged on the liquid injection vertical movement components, three pipe rack components are arranged inside the clamping outer box, a plurality of clamp arm components are arranged on the middle pipe rack component, an oscillation mechanism, a centrifugal mechanism, a position adjustment nitrogen blowing mechanism and a temperature control water bath are arranged inside the clamping outer box, the oscillation mechanism and the temperature control water bath are located on one side of the middle pipe rack component, the centrifugal mechanism and the position adjustment nitrogen blowing mechanism are located on the other side of the middle pipe rack component, a position adjustment camera is arranged on the front side of the position adjustment nitrogen blowing mechanism, and an electric control screen is arranged on the outside of the clamping outer box.

[0012] The working principle of the present invention is as follows: a plurality of purification tubes and centrifuge tubes are placed on the tube rack assembly, 5 g of meat paste crushed from the edible part of the aquatic product is taken, accurately weighed, placed on the middle tube rack assembly and placed in one of the centrifuge tubes, and a plurality of purification tubes placed on the left tube rack assembly are provided with a hydrophilic PTFE filter.

[0013] 1% glacial acetic acid solution and acetonitrile solution are respectively placed in the centrifuge tubes placed on the right tube rack assembly, and anhydrous MaSO4 and anhydrous NaQAc extraction bag powders are respectively placed in the powder quantitative mechanism;

[0014] The lateral movement component drives the longitudinal movement component to move horizontally, and the longitudinal movement component drives the material discharge vertical movement component, the switching vertical movement component and the liquid injection vertical movement component to move longitudinally. The material discharge vertical movement component drives the powder quantitative mechanism to move vertically to transport the material, and the switching vertical movement component drives the switching clamping component to move vertically to clamp the centrifuge tube and remove the screw cap of the centrifuge tube. The liquid injection vertical movement component drives the liquid extraction and injection mechanism to move vertically to extract and discharge the liquid;

[0015] The lateral movement component, the longitudinal movement component and the switching vertical movement component cooperate, the clamp arm component clamps the centrifuge tube to prevent the centrifuge tube from rotating, the screw cap of the centrifuge tube placed on the middle tube rack component is unscrewed, the lateral movement component and the longitudinal movement component cooperate, the pumping and injection mechanism is moved to the top of the centrifuge tube filled with 1% glacial acetic acid solution, the injection vertical movement component drives the pumping and injection mechanism to move downward, extracts 15mL of 1% glacial acetic acid solution, and then rises in the opposite direction and moves to the top of the centrifuge tube with the screw cap opened, and then 15mL of 1% glacial acetic acid solution is injected into the centrifuge tube, and then the screw cap is screwed on the cover, the lateral movement component, the longitudinal movement component and the switching vertical movement component cooperate, the centrifuge tube is placed on the oscillation mechanism, vortex oscillation is performed, and the powder is fully infiltrated;

[0016] Place the centrifuge tube on the middle tube rack assembly, clamp the centrifuge tube with the clamp arm assembly to prevent the centrifuge tube from rotating, unscrew the screw cap of the centrifuge tube, cooperate with the transverse movement assembly, longitudinal movement assembly, injection vertical movement assembly and another extraction and injection mechanism, accurately add 15mL of acetonitrile to the centrifuge tube, and then screw the screw cap on the cover, cooperate with the transverse movement assembly, longitudinal movement assembly and switching vertical movement assembly, place the centrifuge tube on the oscillation mechanism, vortex oscillation, and shake it evenly;

[0017] The transverse movement component, the longitudinal movement component and the switching vertical movement component are coordinated to place the centrifuge tube in a thermostatic water bath, the temperature of the thermostatic water bath is adjusted to 0 degrees Celsius, and the tube is cooled in the water bath for 10 minutes; at this time, the above operation is repeated, and multiple treatments can be performed simultaneously;

[0018] Place the centrifuge tube on the middle tube rack assembly, clamp the centrifuge tube with the clamp arm assembly to prevent the centrifuge tube from rotating, unscrew the screw cap of the centrifuge tube, cooperate with the lateral movement assembly, longitudinal movement assembly, material discharge vertical movement assembly and powder quantitative mechanism, add anhydrous MaSO4 and anhydrous NaQAc extraction bag powder respectively, then screw the screw cap on the cover, cooperate with the lateral movement assembly, longitudinal movement assembly and switching vertical movement assembly, place the centrifuge tube on the oscillation mechanism, vortex oscillation, and oscillate vigorously (500 times / min) for minutes to fully vortex the matrix and the solvent;

[0019] Place the centrifuge tube inside the centrifuge mechanism and centrifuge (4000 revolutions per minute) for 5 minutes (you can also increase the speed and appropriately reduce the centrifugation time. Be careful not to exceed the upper limit of the centrifuge tube tolerance. If there is equipment, low-temperature centrifugation can be used for better results);

[0020] The centrifuge tube is placed on the middle tube rack assembly, and the clamp arm assembly clamps the centrifuge tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed, and 9 mL of supernatant is added to the purification tube through the cooperation of the lateral movement assembly, the longitudinal movement assembly, the injection vertical movement assembly and the other extraction and injection mechanism, and then the screw cap of the centrifuge tube is screwed into the cover, and the lateral movement assembly, the longitudinal movement assembly and the switching vertical movement assembly are cooperated to place the centrifuge tube on the left tube rack assembly. This is waste, and the waste liquid is uniformly collected and processed. The screw cap of the purification tube is screwed into the cover, and the lateral movement assembly, the longitudinal movement assembly and the switching vertical movement assembly are cooperated to place the purification tube on the oscillation mechanism, vortex oscillation and shake well, and vibrate violently (500 times / min) minutes to purify completely. The lateral movement assembly, the longitudinal movement assembly and the switching vertical movement assembly cooperate to place the purification tube inside the centrifugal mechanism, and centrifuge (4000 revolutions per minute) for 5 minutes (after purification by the purification tube, the decolorization effect is obvious as can be seen by the naked eye);

[0021] The purification tube is placed on the middle tube rack assembly, and the clamp arm assembly clamps the purification tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed. Through the cooperation of the lateral movement assembly, the longitudinal movement assembly, the injection vertical movement assembly and another extraction and injection mechanism, 5 mL of supernatant is accurately sucked and added to the inside of the new purification tube. The lateral movement assembly, the longitudinal movement assembly and the switching vertical movement assembly cooperate to place the purification tube on the position adjustment nitrogen blowing mechanism, and blow nitrogen in a water bath (not higher than 40 degrees Celsius). Through the observation of the position adjustment camera, the liquid inside the purification tube is concentrated to 0.4 mL;

[0022] Then place the purification tube on the middle tube rack assembly, cooperate with the transverse movement assembly, longitudinal movement assembly, liquid injection vertical movement assembly and extraction and injection mechanism, add acetonitrile into the purification tube, accurately fill it to 1mL, screw the screw cap of the purification tube on the cover, cooperate with the transverse movement assembly, longitudinal movement assembly and switching vertical movement assembly, place the purification tube on the oscillation mechanism, and vortex oscillate to shake it evenly;

[0023] Then the purification tube is placed on the middle tube rack assembly, and the transverse movement assembly, longitudinal movement assembly, liquid injection vertical movement assembly and pumping and discharging mechanism cooperate to take out the supernatant and move it to several purification tubes placed on the left tube rack assembly. The pumping and discharging mechanism contacts the hydrophilic PTFE filter to filter and obtain the filtrate inside the purification tube. The staff takes it out and puts it inside the sample bottle for on-machine testing.

[0024] Screw the screw cap of the purification tube onto the cover, cooperate with the transverse movement assembly, longitudinal movement assembly and switching vertical movement assembly, and place the purification tube on the pipe rack assembly on the left. This is waste material and waste liquid are collected and processed uniformly.

[0025] The transverse movement assembly includes two sets of slide rail racks and four transverse movement slides. The two sets of slide rail racks are respectively fixed on both sides of the upper end of the clamping outer box. The transverse movement slides are respectively slidably set on the corresponding slide rail racks. Transverse movement motors are fixed on two of the transverse movement slides. Transverse movement gears are fixed on the output shafts of the transverse movement motors. The transverse movement gears are meshed with the slide rail racks.

[0026] With the above structure, the output shaft of the transverse motor rotates, driving the transverse gear to rotate. The transverse gear engages with the slide rail rack, driving the transverse slide to slide on the corresponding slide rail rack, thereby driving the longitudinal movement component to move horizontally.

[0027] The longitudinal movement assembly includes a longitudinal movement beam and a longitudinal movement slide. The longitudinal movement beam is fixed at the upper ends of two transverse movement slides in relative positions. The sides of the longitudinal movement beam are respectively fixed with longitudinal movement rails and longitudinal movement racks. The longitudinal movement slide is slidably set on the longitudinal movement rails. The longitudinal movement slide is fixed with a longitudinal movement motor. A longitudinal movement gear is fixed on the output shaft of the longitudinal movement motor, and the longitudinal movement gear is meshed with the longitudinal movement rack.

[0028] With the above structure, the output shaft of the longitudinal shift motor rotates, driving the longitudinal shift gear to rotate. The longitudinal shift gear engages with the longitudinal shift rack, driving the longitudinal shift slide to slide longitudinally on the corresponding longitudinal shift rack, thereby driving the switching vertical shift assembly, the injection vertical shift assembly and the discharge vertical shift assembly to move longitudinally.

[0029] The switching vertical shift assembly and the liquid injection vertical shift assembly have the same structure as the material discharge vertical shift assembly. The material discharge vertical shift assembly includes a vertical shift slide and a vertical shift motor. The vertical shift slide is fixed on the longitudinal shift slide. A vertical shift slide rod is slidably provided inside the vertical shift slide. A vertical shift rack is fixed on the side of the vertical shift slide rod. A vertical shift gear is fixed on the output shaft of the vertical shift motor. The vertical shift gear is meshed with the vertical shift rack. A tube plate is fixed to the lower end of the vertical shift slide rod of the material discharge vertical shift assembly.

[0030] With the above structure, the output shaft of the vertical motor rotates, driving the vertical gear to rotate. The vertical gear engages with the vertical rack, driving the vertical slide rod to slide vertically inside the vertical slide seat, thereby driving the switching clamping assembly, the extraction and injection mechanism and the powder quantitative mechanism to move vertically.

[0031] The switching clamping assembly includes a switching motor, which is fixed to the lower end of the vertical sliding rod of the switching vertical moving assembly. A switching seat is fixed on the output shaft of the switching motor. The cross-section of the switching seat is an isosceles right triangle. Electric chucks are fixed on both end faces of the switching seat, and chuck claws are provided on the electric chuck.

[0032] With the above structure, the output shaft of the switching motor rotates intermittently, thereby driving the switching seat to rotate intermittently, driving the electric chuck to rotate, adjusting the angle and position of the electric chuck rotation, and the chuck jaws are used to clamp the tube body and the screw cap to meet the needs of moving the tube body and rotating to pick up and place the screw cap.

[0033] The powder quantitative mechanism includes a fixed frame, which is fixed to the upper end of the vertical sliding rod of the material discharge vertical movement assembly at a corresponding position, and a discharge hopper is fixed on the fixed frame. A bearing seat, a feeding hopper, a reducer and a reduction motor are fixed to the upper end of the discharge hopper. A rotating shaft is arranged inside the bearing seat, and the output shaft of the reduction motor is drivingly connected with the input shaft of the reducer, and the output shaft of the reducer is fixedly connected with the upper end of the rotating shaft. A plurality of material shifting frames are fixed on the rotating shaft, and the feeding hopper is connected with the discharge hopper. The discharge hopper is cone-shaped, and a spiral discharging rod is fixed to the lower end of the rotating shaft, and the spiral discharging rod extends out of the discharge hopper. A material guide pipe is fixed to the lower end of the discharge hopper, and the material guide pipe passes through the tube plate at the corresponding position.

[0034] With the above structure, the powder is added from the feeding hopper to the discharging hopper, the output shaft of the reduction motor drives the input shaft of the reducer to rotate, the output shaft of the reducer rotates intermittently, drives the rotating shaft to rotate intermittently, the rotating shaft drives the material shifting rack to shift the material, drives the spiral discharging rod to rotate, when rotating, the powder is discharged from the spiral discharging rod, discharged into the guide pipe, and injected into the centrifuge tube from the guide pipe.

[0035] The pipe rack assembly includes a base plate, which is fixed to the bottom of the clamping outer box. A pipe rack is fixed to the upper end of the base plate, and a plurality of pipe holes with different inner diameters are provided on the pipe rack. The clamping arm assembly includes a clamping seat, which is fixed to the upper end of the base plate of the intermediate pipe rack assembly. A clamping push rod is fixed to the side of the clamping seat, and an adjusting gear is fixed to the telescopic end of the clamping push rod. Two clamping gears are provided for rotation inside the clamping seat, and the clamping gears are all meshed with the adjusting gears. A clamping arc arm is fixed to the end of the clamping gear.

[0036] With the above structure, the centrifuge tube and the purification tube are placed inside the tube placement hole for temporary placement. The telescopic end of the clamping push rod drives the adjusting gear to be pushed out, and the clamping gears are meshed with the adjusting gears to drive the two clamping gears to rotate inside the clamping seat. The clamping gears drive the clamping arc arms at the corresponding positions to close, clamping the centrifuge tube and the purification tube, making it easy to screw in and unscrew the upper end cap.

[0037] The liquid extraction and release injection mechanism includes a fixed base, which is fixed to the lower end of the vertical sliding rod of the liquid injection vertical movement assembly, an injection motor is fixed to the upper end of the fixed base, an injection screw is rotatably arranged inside the fixed base, the output shaft of the injection motor is fixedly connected to the upper end of the injection screw, a lifting screw seat is slidably provided on the fixed base, the lifting screw seat is transmission-connected to the liquid injection screw, a piston rod is fixed on the lifting screw seat, an injection piston is fixed to the lower end of the piston rod, an injection sleeve is fixed to the lower end of the fixed base, and the injection piston is slidably arranged inside the injection sleeve.

[0038] With the above structure, the output shaft of the injection motor drives the injection screw to rotate, the lifting screw seat is transmission-connected with the injection screw, the lifting screw seat slides on the fixed base, drives the piston rod to move up and down, the piston rod drives the injection piston to move up and down, when the injection piston moves up, the liquid is sucked into the injection sleeve, and when the injection piston moves down, the liquid inside the injection sleeve is discharged.

[0039] The oscillation mechanism comprises an oscillation main machine, which is fixed at the bottom of the clamping outer box, an oscillation plate is fixed on the oscillation main machine, an oscillation base is fixed on the oscillation plate, and an oscillation bracket is fixed on the upper end of the oscillation base; the centrifugal mechanism comprises a centrifugal main machine, which is fixed at the bottom of the clamping outer box, a centrifugal motor is fixed inside the centrifugal main machine, a centrifugal placement plate is fixed on the upper end of the output shaft of the centrifugal motor, and a plurality of centrifugal placement tubes evenly distributed around the circumference are arranged on the centrifugal placement plate; a water bath tube is arranged on the upper end of the temperature-controlled water bath.

[0040] With the above structure, the oscillation main unit drives the oscillation disk to oscillate the vortex, thereby driving the oscillation disk and the oscillation base to oscillate the vortex, and the tube body is placed on the oscillation bracket, and the tube body contacts the oscillation base; the output shaft of the centrifugal motor drives the centrifugal placement disk to rotate at high speed, and the tube body is placed inside the centrifugal placement tube for centrifugal rotation, and the temperature-adjusting water bath controls the water temperature inside the water bath, and the tube body is placed inside the water bath for cooling.

[0041] The positioning nitrogen blowing mechanism comprises a positioning screw, a nitrogen blowing main machine and a lifting screw. The positioning screw and the lifting screw are fixed at the bottom of the clamping outer box. A positioning screw motor is fixed on the positioning screw. The output shaft of the positioning screw motor is fixedly connected to the screw end of the positioning screw. A positioning slide is provided on the positioning screw. The nitrogen blowing main machine is slidingly arranged at the bottom of the clamping outer box. The nitrogen blowing main machine is fixed on the side of the positioning slide. A plurality of nitrogen blowing placement tubes are provided on the nitrogen blowing main machine. A water bath is provided inside the nitrogen blowing main machine. The shell of the nitrogen blowing main machine and the water bath are both made of transparent materials. A plurality of nitrogen blowing placement tubes are located inside the water bath. A lifting screw motor is fixed on the lifting screw. The output shaft of the lifting screw motor is fixedly connected to the screw end of the lifting screw. A positioning slide is provided on the lifting screw. A plurality of nitrogen blowing valves are fixed on the positioning slide. The nitrogen blowing valves are all connected to an external nitrogen generator. Nitrogen blowing pipes are provided at the lower ends of the nitrogen blowing valves. The nitrogen blowing pipes are located above the corresponding nitrogen blowing placement pipes.

[0042] With the above structure, the water bath is heated by water bath, and the water bath temperature is not higher than 40 degrees Celsius. The output shaft of the positioning screw motor drives the screw of the positioning screw to rotate, and the positioning screw drives the positioning slide to move. The positioning slide drives the nitrogen blowing host to slide at the bottom of the clamping outer box, so that the nitrogen blowing placement tube moves and is not located directly below the nitrogen blowing tube. The purification tube with the screw cap removed is placed inside the nitrogen blowing placement tube, and then the nitrogen blowing host is reset, and the nitrogen blowing placement tube moves to directly below the nitrogen blowing tube. The output shaft of the lifting screw motor drives the screw of the lifting screw to rotate, thereby driving the positioning slide to descend, the nitrogen blowing valve to descend, and the nitrogen blowing tube extends into the placement tube for nitrogen blowing. The shell of the nitrogen blowing host and the water bath are made of transparent materials, which are convenient for observation through the positioning camera to concentrate the liquid inside the purification tube to 0.1mL.

[0043] Compared with the existing technology, the QuEChERS processing detection device for detecting antibiotics in aquatic products has the following advantages:

[0044] The device can process the aquatic products to be tested in batches for multiple times to conduct antibiotic inspections. The test cycle is short, the pre-treatment method is simple and reliable, the recovery rate is between 75-115%, no special experimental equipment is required, and the test cost is low.

[0045] Through the cooperation of the transverse movement component and the longitudinal movement component, each component can be driven to move horizontally and vertically to meet the processing work requirements;

[0046] The discharge vertical shift assembly cooperates with the powder quantitative mechanism to achieve stable powder injection, the liquid injection vertical shift assembly cooperates with the liquid extraction and discharge mechanism to achieve stable liquid extraction and discharge, the tube body is driven to move by the switching vertical shift assembly and the switching clamping assembly, and the tube body cap is screwed in or unscrewed by cooperating with the clamp arm assembly;

[0047] The oscillating mechanism is used to perform stable and efficient vortex oscillation, the centrifugal mechanism is used to perform fast and stable centrifugal stratification, the position-adjusting nitrogen blowing mechanism is used to achieve nitrogen blowing concentration, and the temperature is adjusted by the temperature-adjusting water bath, which is convenient for water bath cooling;

[0048] The pipe rack assembly is used to place various empty pipes, pipes filled with intermediate liquids and pipes filled with waste liquids, which are convenient to take and place. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is a three-dimensional structural schematic diagram of the present invention.

[0050] Figure 2 It is a schematic diagram of the three-dimensional structure of the present invention when the clamping outer box is removed.

[0051] Figure 3 It is a schematic diagram of the three-dimensional structure of the clamping outer box in the present invention.

[0052] Figure 4 yes Figure 3 Schematic diagram of the enlarged structure at point A in the middle.

[0053] Figure 5 It is a structural schematic diagram of the powder quantitative mechanism in the present invention.

[0054] Figure 6 It is a structural schematic diagram of the pipe rack assembly in the middle position in the present invention.

[0055] Figure 7 It is a structural schematic diagram of the oscillation mechanism in the present invention.

[0056] Figure 8 It is a three-dimensional structural schematic diagram of the centrifugal mechanism in the present invention.

[0057] Fig. 9 It is a three-dimensional structural schematic diagram of the position-adjusting nitrogen blowing mechanism in the present invention.

[0058] Fig.10 It is a three-dimensional structural schematic diagram of the liquid extraction and injection mechanism in the present invention.

[0059] In the figure, 1, clamping outer box; 2, electric control panel; 3, transverse movement assembly; 4, longitudinal movement assembly; 5, material discharge vertical movement assembly; 6, powder quantitative mechanism; 7, oscillation mechanism; 8, liquid injection vertical movement assembly; 9, liquid extraction and injection mechanism; 10, centrifugal mechanism; 11, position adjustment nitrogen blowing mechanism; 12, temperature control water bath; 13, pipe rack assembly; 14, slide rail rack; 15, transverse movement motor; 16, longitudinal movement beam; 17, longitudinal movement slide rail; 18. Longitudinal rack; 19. Longitudinal slide; 20. Vertical motor; 21. Longitudinal motor; 22. Vertical slide; 23. Vertical slide bar; 24. Tube plate; 25. Switching clamping assembly; 26. Vertical rack; 27. Switching motor; 28. Switching seat; 29. ​​Electric chuck; 30. Chuck jaws; 31. Feeding hopper; 32. Speed ​​reducer; 33. Speed ​​reducer motor; 34. Fixed frame; 35. Bearing seat; 36. Material dispensing rack; 37. Screw discharging rod; 38. Discharging hopper; 39. Bottom plate; 40. Tube placement hole; 41. Tube placement rack; 42. Clamping push rod; 43. Clamping seat; 44. Clamping gear; 45. Clamping arc arm; 46. Oscillating main machine; 47. Oscillating plate; 48. Oscillating base; 49. Oscillating bracket; 50. Centrifugal main machine; 51. Centrifugal placement plate; 52. Centrifugal placement tube; 53. Adjusting screw; 54. Positioning slide; 55. Nitrogen blowing main machine; 56. Nitrogen blowing placement tube; 57. Nitrogen blowing tube; 58. Nitrogen blowing valve; 59. Lifting screw; 60. Lifting slide; 61. Positioning screw motor; 62. Liquid injection motor; 63. Fixed base; 64. Liquid injection screw; 65. Liquid injection sleeve; 66. Liquid injection piston; 67. Lifting screw seat; 68. Piston rod; 69. Lifting screw motor; 70. Positioning camera. DETAILED DESCRIPTION

[0060] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0061] like Figure 1-Figure 10As shown, the QuEChERS processing detection device for detecting antibiotics in aquatic products comprises a clamping outer box 1, two transverse moving components 3 are arranged above the clamping outer box 1, and longitudinal moving components 4 are arranged on the transverse moving components 3, a plurality of material discharging vertical moving components 5 are arranged on the side of one of the longitudinal moving components 4, and a powder quantitative mechanism 6 is arranged on the material discharging vertical moving components 5, a switching vertical moving component is arranged on one side of the other longitudinal moving component 4, and a switching clamping component 25 is arranged at the lower end of the switching vertical moving component, and a plurality of liquid injection vertical moving components 8 are arranged on the other side of the other longitudinal moving component 4, and the liquid injection vertical moving components 8 A pumping and discharging liquid injection mechanism 9 is provided on each of the above parts. Three pipe rack assemblies 13 are provided inside the clamping outer box 1. A plurality of clamping arm assemblies are provided on the middle pipe rack assembly 13. An oscillating mechanism 7, a centrifugal mechanism 10, a positioning nitrogen blowing mechanism 11 and a temperature-controlled water bath 12 are provided inside the clamping outer box 1. The oscillating mechanism 7 and the temperature-controlled water bath 12 are located on one side of the middle pipe rack assembly 13, the centrifugal mechanism 10 and the positioning nitrogen blowing mechanism 11 are located on the other side of the middle pipe rack assembly 13, a positioning camera 70 is provided on the front side of the positioning nitrogen blowing mechanism 11, and an electric control screen 2 is provided on the outside of the clamping outer box 1.

[0062] Several purification tubes and centrifuge tubes are placed on the tube rack assembly 13. 5 g of meat paste made from the edible part of the aquatic product is accurately weighed and placed in one of the centrifuge tubes on the middle tube rack assembly 13. Several purification tubes placed on the left tube rack assembly 13 are provided with hydrophilic PTFE filters.

[0063] 1% glacial acetic acid solution and acetonitrile solution are respectively placed in the centrifuge tube placed on the right tube rack assembly 13, and anhydrous MaSO4 and anhydrous NaQAc extraction bag powders are respectively placed in the powder quantitative mechanism 6;

[0064] The lateral movement component 3 drives the longitudinal movement component 4 to move horizontally, and the longitudinal movement component 4 drives the material discharge vertical movement component 5, the switching vertical movement component and the liquid injection vertical movement component 8 to move vertically. The material discharge vertical movement component 5 drives the powder quantitative mechanism 6 to move vertically to transport the material, and the switching vertical movement component drives the switching clamping component 25 to move vertically to clamp the centrifuge tube and remove the screw cap of the centrifuge tube. The liquid injection vertical movement component 8 drives the liquid extraction and injection mechanism 9 to move vertically to extract and discharge the liquid;

[0065] The lateral movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate, the clamp arm component clamps the centrifuge tube to prevent the centrifuge tube from rotating, the screw cap of the centrifuge tube placed on the middle tube rack component 13 is unscrewed, the lateral movement component 3 and the longitudinal movement component 4 cooperate, the extraction and injection mechanism 9 is moved to the top of the centrifuge tube filled with 1% glacial acetic acid solution, the injection vertical movement component 8 drives the extraction and injection mechanism 9 to move downward, extract 15mL of 1% glacial acetic acid solution, and then rises in the opposite direction and moves to the top of the centrifuge tube with the screw cap opened, and then 15mL of 1% glacial acetic acid solution is injected into the centrifuge tube, and then the screw cap is screwed on the cover, the lateral movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate, the centrifuge tube is placed on the oscillation mechanism 7, vortex oscillation, so that the powder is fully infiltrated;

[0066] Place the centrifuge tube on the middle tube rack assembly 13, clamp the centrifuge tube with the clamp arm assembly to prevent the centrifuge tube from rotating, unscrew the screw cap of the centrifuge tube, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4, injection vertical movement assembly 8 and another extraction and injection mechanism 9, accurately add 15mL of acetonitrile to the centrifuge tube, and then screw the screw cap on the cover, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4 and switching vertical movement assembly, place the centrifuge tube on the oscillation mechanism 7, vortex oscillation, and shake it evenly;

[0067] The transverse movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate to place the centrifuge tube in the temperature-controlled water bath 12, adjust the temperature of the temperature-controlled water bath 12 to 0 degrees Celsius, and cool it in the water bath for 10 minutes; repeat the above operation at this time;

[0068] Place the centrifuge tube on the middle tube rack assembly 13, clamp the centrifuge tube with the clamp arm assembly to prevent the centrifuge tube from rotating, unscrew the screw cap of the centrifuge tube, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4, material discharge vertical movement assembly 5 and powder quantitative mechanism 6, add anhydrous MaSO4 and anhydrous NaQAc extraction bag powder respectively, then screw the screw cap on the cover, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4 and switch vertical movement assembly, place the centrifuge tube on the oscillation mechanism 7, vortex oscillation, and vigorously oscillate (500 times / min) for 3 minutes to fully vortex the matrix and the solvent;

[0069] Place the centrifuge tube inside the centrifuge mechanism 10 and centrifuge (4000 revolutions per minute) for 5 minutes (the speed can also be increased, and the centrifugation time can be appropriately reduced. Be careful not to exceed the upper limit of the centrifuge tube tolerance. If there is equipment, low-temperature centrifugation can be performed for better results);

[0070] The centrifuge tube is placed on the middle tube rack assembly 13, and the clamp arm assembly clamps the centrifuge tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed, and 9 mL of supernatant is added to the purification tube through the cooperation of the transverse movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and another extraction and injection mechanism 9, and then the screw cap of the centrifuge tube is screwed on. The transverse movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly are cooperated to place the centrifuge tube on the tube rack assembly 13 on the left. This is Waste materials and waste liquids are collected and processed uniformly. The screw cap of the purification tube is screwed onto the cap. The transverse moving assembly 3, the longitudinal moving assembly 4 and the switching vertical moving assembly cooperate to place the purification tube on the oscillation mechanism 7, vortex oscillation and shake evenly. Vigorously oscillate (500 times / min) for 5 minutes to purify completely. The transverse moving assembly 3, the longitudinal moving assembly 4 and the switching vertical moving assembly cooperate to place the purification tube inside the centrifugal mechanism 10 and centrifuge (4000 revolutions per minute) for 5 minutes (after purification by the purification tube, the decolorization effect is obvious to the naked eye);

[0071] The purification tube is placed on the middle tube rack assembly 13, and the clamp arm assembly clamps the purification tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed. Through the cooperation of the lateral movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and another extraction and injection mechanism 9, 5 mL of supernatant is accurately sucked and added to the inside of the new purification tube. The lateral movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly are cooperated to place the purification tube on the position adjustment nitrogen blowing mechanism 11, and nitrogen is blown in a water bath (not higher than 40 degrees Celsius). Through the observation of the position adjustment camera 70, the liquid inside the purification tube is concentrated to 0.4 mL;

[0072] Then place the purification tube on the middle tube rack assembly 13, cooperate with the transverse movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and the extraction and injection mechanism 9, add acetonitrile into the purification tube, accurately fill it to 1mL, screw the screw cap of the purification tube on the cover, cooperate with the transverse movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly, place the purification tube on the oscillation mechanism 7, and vortex oscillate to shake it evenly;

[0073] Then the purification tube is placed on the middle tube rack assembly 13, and the transverse movement assembly 3, the longitudinal movement assembly 4, the liquid injection vertical movement assembly 8 and the pumping and discharging mechanism 9 cooperate to take out the supernatant and move it to the purification tubes placed on the left tube rack assembly 13. The pumping and discharging mechanism 9 contacts the hydrophilic PTFE filter to filter and obtain the filtrate inside the purification tube. The staff takes it out and puts it inside the sample bottle for ultra-high performance liquid chromatography-tandem mass spectrometry detection.

[0074] The screw cap of the purification tube is screwed onto the cover, and the transverse movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate to place the purification tube on the pipe rack component 13 on the left side. This is waste material and waste liquid are collected and processed uniformly.

[0075] The transverse movement assembly 3 includes two sets of slide rail racks 14 and four transverse movement slides. The two sets of slide rail racks 14 are respectively fixed on both sides of the upper end of the clamping outer box 1. The transverse movement slides are respectively slidably set on the corresponding slide rail racks 14. Transverse movement motors 15 are fixed on two of the transverse movement slides. A transverse movement gear is fixed on the output shaft of the transverse movement motor 15, and the transverse movement gear is meshed with the slide rail racks 14.

[0076] The output shaft of the transverse motor 15 rotates, driving the transverse gear to rotate. The transverse gear meshes with the slide rail rack 14, driving the transverse slide to slide on the corresponding slide rail rack 14, thereby driving the longitudinal movement component 4 to move horizontally.

[0077] The longitudinal movement assembly 4 includes a longitudinal movement beam 16 and a longitudinal movement slide 19. The longitudinal movement beam 16 is fixed at the upper ends of two transverse movement slides in relative positions. The sides of the longitudinal movement beam 16 are respectively fixed with longitudinal movement rails 17 and longitudinal movement racks 18. The longitudinal movement slide 19 is slidably set on the longitudinal movement rails 17. The longitudinal movement slide 19 is fixed with a longitudinal movement motor 21. A longitudinal movement gear is fixed on the output shaft of the longitudinal movement motor 21, and the longitudinal movement gear is meshed with the longitudinal movement rack 18.

[0078] The output shaft of the longitudinal shift motor 21 rotates, driving the longitudinal shift gear to rotate. The longitudinal shift gear engages with the longitudinal shift rack 18, driving the longitudinal shift slide 19 to slide longitudinally on the corresponding longitudinal shift rack 18, thereby driving the switching vertical shift assembly, the injection vertical shift assembly 8 and the discharge vertical shift assembly 5 to move longitudinally.

[0079] The switching vertical shift assembly and the liquid injection vertical shift assembly 8 have the same structure as the material discharge vertical shift assembly 5. The material discharge vertical shift assembly 5 includes a vertical shift slide 22 and a vertical shift motor 20. The vertical shift slide 22 is fixed on the longitudinal shift slide 19. A vertical shift slide rod 23 is slidably provided inside the vertical shift slide 22. A vertical shift rack 26 is fixed to the side of the vertical shift slide rod 23. A vertical shift gear is fixed to the output shaft of the vertical shift motor 20. The vertical shift gear is meshed with the vertical shift rack 26. A tube plate 24 is fixed to the lower end of the vertical shift slide rod 23 of the material discharge vertical shift assembly 5.

[0080] The output shaft of the vertical motor 20 rotates, driving the vertical gear to rotate. The vertical gear engages with the vertical rack 26, driving the vertical slide rod 23 to slide vertically inside the vertical slide seat 22, thereby driving the switching clamping assembly 25, the extraction and injection mechanism 9 and the powder quantitative mechanism 6 to move vertically.

[0081] The switching clamping assembly 25 includes a switching motor 27, which is fixed to the lower end of the vertical sliding rod 23 of the switching vertical moving assembly. A switching seat 28 is fixed on the output shaft of the switching motor 27. The cross-section of the switching seat 28 is an isosceles right triangle. Electric chucks 29 are fixed to both end faces of the switching seat 28, and a chuck clamping claw 30 is provided on the electric chuck 29.

[0082] The output shaft of the switching motor 27 rotates intermittently, thereby driving the switching seat 28 to rotate intermittently, driving the electric chuck 29 to rotate, adjusting the rotation angle and position of the electric chuck 29, and the chuck jaws 30 are used to clamp the tube body and the screw cap to meet the needs of moving the tube body and rotating to pick up and put the screw cap.

[0083] The powder quantitative mechanism 6 includes a fixed frame 34, which is fixed to the upper end of the vertical sliding rod 23 of the material discharge vertical movement assembly 5 at the corresponding position. A discharge hopper 38 is fixed on the fixed frame 34. A bearing seat 35, a feeding hopper 31, a reducer 32 and a reduction motor 33 are fixed to the upper end of the discharge hopper 38. A rotating shaft is provided inside the bearing seat 35. The output shaft of the reduction motor 33 is drivingly connected to the input shaft of the reducer 32. The output shaft of the reducer 32 is fixedly connected to the upper end of the rotating shaft. A plurality of material shifting frames 36 are fixed on the rotating shaft. The feeding hopper 31 is connected to the discharge hopper 38. The discharge hopper 38 is cone-shaped. A spiral discharge rod 37 is fixed to the lower end of the rotating shaft. The spiral discharge rod 37 extends out of the discharge hopper 38. A material guide pipe is fixed to the lower end of the discharge hopper 38. The material guide pipe passes through the tube plate 24 at the corresponding position.

[0084] The powder is fed into the discharge hopper 38 from the feeding hopper 31, and the output shaft of the reduction motor 33 drives the input shaft of the reducer 32 to rotate. The output shaft of the reducer 32 rotates intermittently, driving the rotating shaft to rotate intermittently. The rotating shaft drives the material shifting rack 36 to shift the material, and drives the spiral discharging rod 37 to rotate. During the rotation, the powder is discharged from the spiral discharging rod 37, discharged into the guide pipe, and injected into the centrifuge tube from the guide pipe.

[0085] The pipe rack assembly 13 includes a base plate 39, which is fixed to the bottom of the clamping outer box 1. A pipe rack 41 is fixed to the upper end of the base plate 39. The pipe rack 41 is provided with a plurality of pipe holes 40 with different inner diameters. The clamping arm assembly includes a clamping seat 43, which is fixed to the upper end of the base plate 39 of the intermediate pipe rack assembly 13. A clamping push rod 42 is fixed to the side of the clamping seat 43. An adjusting gear is fixed to the telescopic end of the clamping push rod 42. Two clamping gears 44 are provided for rotation inside the clamping seat 43. The clamping gears 44 are all meshed with the adjusting gears. A clamping arc arm 45 is fixed to the end of the clamping gear 44.

[0086] The centrifuge tube and the purification tube are placed inside the tube placement hole 40 for temporary placement. The telescopic end of the clamping push rod 42 drives the adjusting gear to be pushed out, and the clamping gears 44 are meshed with the adjusting gears, driving the two clamping gears 44 to rotate inside the clamping seat 43. The clamping gears 44 drive the clamping arc arms 45 at the corresponding positions to close, clamping the centrifuge tube and the purification tube, making it easy to screw in and unscrew the upper end cap.

[0087] The liquid extraction and release mechanism 9 includes a fixed base 63, which is fixed to the lower end of the vertical sliding rod 23 of the liquid injection vertical movement assembly 8. An injection motor 62 is fixed to the upper end of the fixed base 63. An injection screw 64 is rotatably arranged inside the fixed base 63. The output shaft of the injection motor 62 is fixedly connected to the upper end of the injection screw 64. A lifting screw seat 67 is slidably provided on the fixed base 63. The lifting screw seat 67 is transmission-connected to the injection screw 64. A piston rod 68 is fixed to the lifting screw seat 67. An injection piston 66 is fixed to the lower end of the piston rod 68. An injection sleeve 65 is fixed to the lower end of the fixed base 63. The injection piston 66 is slidably arranged inside the injection sleeve 65.

[0088] The output shaft of the injection motor 62 drives the injection screw 64 to rotate, and the lifting screw seat 67 is transmission-connected to the injection screw 64. The lifting screw seat 67 slides on the fixed base 63, driving the piston rod 68 to move up and down. The piston rod 68 drives the injection piston 66 to move up and down. When the injection piston 66 moves up, liquid is sucked into the injection sleeve 65. When the injection piston 66 moves down, the liquid inside the injection sleeve 65 is discharged.

[0089] The oscillation mechanism 7 includes an oscillation main unit 46, which is fixed to the bottom of the clamping outer box 1, an oscillation plate 47 is fixed on the oscillation main unit 46, an oscillation base 48 is fixed on the oscillation plate 47, and an oscillation bracket 49 is fixed to the upper end of the oscillation base 48; the centrifugal mechanism 10 includes a centrifugal main unit 50, which is fixed to the bottom of the clamping outer box 1, a centrifugal motor is fixed inside the centrifugal main unit 50, a centrifugal placement plate 51 is fixed to the upper end of the output shaft of the centrifugal motor, and a plurality of centrifugal placement tubes 52 evenly distributed around the circumference are provided on the centrifugal placement plate 51; a water bath tube is provided at the upper end of the temperature-controlled water bath 12.

[0090] The oscillating main unit 46 drives the oscillating disk 47 to oscillate the vortex, thereby driving the oscillating disk 47 and the oscillating base 48 to oscillate the vortex, placing the tube body on the oscillating bracket 49, and the tube body abuts against the oscillating base 48; the output shaft of the centrifugal motor drives the centrifugal placement disk 51 to rotate at high speed, and the tube body is placed inside the centrifugal placement tube 52 for centrifugal rotation, and the temperature-controlled water bath 12 controls the water temperature inside the water bath, and the tube body is placed inside the water bath for cooling treatment.

[0091] The positioning nitrogen blowing mechanism 11 includes a positioning screw 53, a nitrogen blowing main machine 55, and a lifting screw 59. The positioning screw 53 and the lifting screw 59 are fixed at the bottom of the clamping outer box 1. A positioning screw motor 61 is fixed on the positioning screw 53. The output shaft of the positioning screw motor 61 is fixedly connected to the screw end of the positioning screw 53. A positioning slide 54 is provided on the positioning screw 53. The nitrogen blowing main machine 55 is slidably arranged at the bottom of the clamping outer box 1. The nitrogen blowing main machine 55 is fixed on the side of the positioning slide 54. A plurality of nitrogen blowing placement tubes 56 are provided on the nitrogen blowing main machine 55. A water bath is provided inside, and the shell of the nitrogen blowing main unit 55 and the water bath are made of transparent materials. Several nitrogen blowing placement tubes 56 are located inside the water bath. A lifting screw motor 69 is fixed on the lifting screw 59. The output shaft of the lifting screw motor 69 is fixedly connected to the screw end of the lifting screw 59. A positioning slide 54 is provided on the lifting screw 59 for transmission. Several nitrogen blowing valves 58 are fixed on the positioning slide 54. The nitrogen blowing valves 58 are all connected to an external nitrogen generator. Nitrogen blowing tubes 57 are provided at the lower ends of the nitrogen blowing valves 58. The nitrogen blowing tubes 57 are located above the corresponding nitrogen blowing placement tubes 56.

[0092] The water bath is heated in a water bath, and the water bath temperature is not higher than 40 degrees Celsius. The output shaft of the positioning screw motor 61 drives the screw of the positioning screw 53 to rotate, and the positioning screw 53 drives the positioning slide 54 to move, and the positioning slide 54 drives the nitrogen blowing host 55 to slide at the bottom of the clamping outer box 1, so that the nitrogen blowing placement tube 56 moves and is not located directly below the nitrogen blowing tube 57. The purification tube with the screw cap removed is placed inside the nitrogen blowing placement tube 56, and then the nitrogen blowing host 55 is reset, and the nitrogen blowing placement tube 56 moves to directly below the nitrogen blowing tube 57. The output shaft of the lifting screw motor 69 drives the screw of the lifting screw 59 to rotate, thereby driving the positioning slide 54 to descend, the nitrogen blowing valve 58 to descend, and the nitrogen blowing tube 57 to extend into the placement tube 56 for nitrogen blowing. The outer shell of the nitrogen blowing host 55 and the water bath are made of transparent materials, which are convenient for observation through the positioning camera 70 to concentrate the liquid inside the purification tube to 0.4mL.

[0093] The working principle of the present invention is as follows: the working time of each electrical component is set through the electronic control panel 2, that is, the working sequence is controlled. A plurality of purification tubes and centrifuge tubes are placed on the tube rack assembly 13. 5 g of meat paste crushed from the edible part of the aquatic product is taken, accurately weighed, placed on the middle tube rack assembly 13 and placed in one of the centrifuge tubes. A hydrophilic PTFE filter is provided on the plurality of purification tubes placed on the left tube rack assembly 13.

[0094] 1% glacial acetic acid solution and acetonitrile solution are respectively placed in the centrifuge tube placed on the right tube rack assembly 13, and anhydrous MaSO4 and anhydrous NaQAc extraction bag powders are respectively placed in the powder quantitative mechanism 6;

[0095] The output shaft of the transverse movement motor 15 rotates, driving the transverse movement gear to rotate, and the transverse movement gear meshes with the slide rail rack 14, driving the transverse movement slide to slide on the corresponding slide rail rack 14, thereby driving the longitudinal movement component 4 to move horizontally; the output shaft of the longitudinal movement motor 21 rotates, driving the longitudinal movement gear to rotate, and the longitudinal movement gear meshes with the longitudinal movement rack 18, driving the longitudinal movement slide 19 to slide longitudinally on the corresponding longitudinal movement rack 18, thereby driving the switching vertical movement component, the injection vertical movement component 8 and the discharge vertical movement component 5 to move longitudinally; the output shaft of the vertical movement motor 20 rotates, driving the vertical movement gear to rotate, and the vertical movement gear meshes with the vertical movement rack 26, driving the vertical movement slide 23 to slide vertically inside the vertical movement slide 22, thereby driving the switching clamping component 25, the extraction and injection mechanism 9 and the powder quantitative mechanism 6 to move vertically;

[0096] The material discharge vertical shift assembly 5 drives the powder material quantitative mechanism 6 to move vertically to feed the material, the switching vertical shift assembly drives the switching clamping assembly 25 to move vertically to clamp the centrifuge tube and remove the screw cap of the centrifuge tube, and the liquid injection vertical shift assembly 8 drives the liquid extraction and injection mechanism 9 to move vertically to extract and discharge the liquid;

[0097] The lateral movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate, the clamp arm component clamps the centrifuge tube to prevent the centrifuge tube from rotating, the screw cap of the centrifuge tube placed on the middle tube rack component 13 is unscrewed, the lateral movement component 3 and the longitudinal movement component 4 cooperate, the extraction and injection mechanism 9 is moved to the top of the centrifuge tube filled with 1% glacial acetic acid solution, the injection vertical movement component 8 drives the extraction and injection mechanism 9 to move downward, extract 15mL of 1% glacial acetic acid solution, and then rises in the opposite direction and moves to the top of the centrifuge tube with the screw cap opened, and then 15mL of 1% glacial acetic acid solution is injected into the centrifuge tube, and then the screw cap is screwed on the cover, the lateral movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate, the centrifuge tube is placed on the oscillation mechanism 7, vortex oscillation, so that the powder is fully infiltrated;

[0098] Place the centrifuge tube on the middle tube rack assembly 13, clamp the centrifuge tube with the clamp arm assembly to prevent the centrifuge tube from rotating, unscrew the screw cap of the centrifuge tube, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4, injection vertical movement assembly 8 and another extraction and injection mechanism 9, accurately add 15mL of acetonitrile to the centrifuge tube, and then screw the screw cap on the cover, cooperate with the lateral movement assembly 3, longitudinal movement assembly 4 and switching vertical movement assembly, place the centrifuge tube on the oscillation mechanism 7, vortex oscillation, and shake it evenly;

[0099] The transverse movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate to place the centrifuge tube in the temperature-controlled water bath 12, adjust the temperature of the temperature-controlled water bath 12 to 0 degrees Celsius, and cool it in the water bath for 10 minutes; repeat the above operation at this time;

[0100] The centrifuge tube is placed on the middle tube rack assembly 13, the clamp arm assembly clamps the centrifuge tube to prevent the centrifuge tube from rotating, and the screw cap of the centrifuge tube is unscrewed. The lateral movement assembly 3, the longitudinal movement assembly 4, the material discharge vertical movement assembly 5 and the powder quantitative mechanism 6 cooperate, and the powder is added from the feeding hopper 31 to the discharge hopper 38. The output shaft of the reduction motor 33 drives the input shaft of the reducer 32 to rotate, and the output shaft of the reducer 32 rotates intermittently, driving the rotating shaft to rotate intermittently, and the rotating shaft drives the material-pickup rack 36 to pick the material, and drives the spiral discharging rod 37 to rotate. During rotation, the powder is discharged from the spiral discharging rod 37 and discharged into the guide pipe, and injected into the centrifuge tube from the guide pipe, and anhydrous MaSO4 and anhydrous NaQAc extraction bag powders are added respectively, and then the screw cap is screwed into the cover, and the lateral movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly cooperate, and the centrifuge tube is placed on the oscillation mechanism 7, vortex oscillation, and violent oscillation (500 times / min) for 3 minutes to fully vortex the matrix and the solvent;

[0101] Place the centrifuge tube inside the centrifuge mechanism 10 and centrifuge (4000 revolutions per minute) for 5 minutes (the speed can also be increased, and the centrifugation time can be appropriately reduced. Be careful not to exceed the upper limit of the centrifuge tube tolerance. If there is equipment, low-temperature centrifugation can be performed for better results);

[0102] The centrifuge tube is placed on the middle tube rack assembly 13, and the clamp arm assembly clamps the centrifuge tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed, and 9 mL of supernatant is added to the purification tube through the cooperation of the transverse movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and another extraction and injection mechanism 9, and then the screw cap of the centrifuge tube is screwed on. The transverse movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly are cooperated to place the centrifuge tube on the tube rack assembly 13 on the left. This is Waste materials and waste liquids are collected and processed uniformly. The screw cap of the purification tube is screwed onto the cap. The transverse moving assembly 3, the longitudinal moving assembly 4 and the switching vertical moving assembly cooperate to place the purification tube on the oscillation mechanism 7, vortex oscillation and shake evenly. Vigorously oscillate (500 times / min) for 5 minutes to purify completely. The transverse moving assembly 3, the longitudinal moving assembly 4 and the switching vertical moving assembly cooperate to place the purification tube inside the centrifugal mechanism 10 and centrifuge (4000 revolutions per minute) for 5 minutes (after purification by the purification tube, the decolorization effect is obvious to the naked eye);

[0103] The purification tube is placed on the middle tube rack assembly 13, and the clamp arm assembly clamps the purification tube to prevent the centrifuge tube from rotating. The screw cap of the centrifuge tube and the screw cap of one of the purification tubes are unscrewed, and 5 mL of supernatant is accurately sucked and added to the new purification tube through the cooperation of the transverse movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and another extraction and injection mechanism 9. The transverse movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly are cooperated to place the purification tube on the positioning nitrogen blowing mechanism 11, and the water bath is heated with water bath, and the water bath temperature is not higher than 40 degrees Celsius. The output shaft of the positioning screw motor 61 drives the screw of the positioning screw 53 to rotate, and the positioning screw 53 drives the positioning slide 54 to move, and the positioning slide 5 4 drives the nitrogen blowing host 55 to slide at the bottom of the clamping outer box 1, so that the nitrogen blowing placement tube 56 moves and is not located directly under the nitrogen blowing tube 57, and the purification tube with the screw cap removed is placed inside the nitrogen blowing placement tube 56, and then the nitrogen blowing host 55 is reset, and the nitrogen blowing placement tube 56 moves to the directly below the nitrogen blowing tube 57, and the output shaft of the lifting screw motor 69 drives the screw of the lifting screw 59 to rotate, thereby driving the adjustment slide 54 to descend, the nitrogen blowing valve 58 to descend, and the nitrogen blowing tube 57 extends into the placement tube 56 to perform nitrogen blowing. The shell and water bath of the nitrogen blowing host 55 are made of transparent materials, which are convenient for observation through the adjustment camera 70, and the liquid inside the purification tube is concentrated to 0.4mL;

[0104] Then place the purification tube on the middle tube rack assembly 13, cooperate with the transverse movement assembly 3, the longitudinal movement assembly 4, the injection vertical movement assembly 8 and the extraction and injection mechanism 9, add acetonitrile into the purification tube, accurately fill it to 1mL, screw the screw cap of the purification tube on the cover, cooperate with the transverse movement assembly 3, the longitudinal movement assembly 4 and the switching vertical movement assembly, place the purification tube on the oscillation mechanism 7, and vortex oscillate to shake it evenly;

[0105] Then the purification tube is placed on the middle tube rack assembly 13, and the transverse movement assembly 3, the longitudinal movement assembly 4, the liquid injection vertical movement assembly 8 and the pumping and discharging mechanism 9 cooperate to take out the supernatant and move it to the purification tubes placed on the left tube rack assembly 13. The pumping and discharging mechanism 9 contacts the hydrophilic PTFE filter to filter and obtain the filtrate inside the purification tube. The staff takes it out and puts it inside the sample bottle for ultra-high performance liquid chromatography-tandem mass spectrometry detection.

[0106] The screw cap of the purification tube is screwed onto the cover, and the transverse movement component 3, the longitudinal movement component 4 and the switching vertical movement component cooperate to place the purification tube on the pipe rack component 13 on the left side. This is waste material and waste liquid are collected and processed uniformly.

[0107] In summary, this device can process aquatic products to be tested in batches for multiple times to conduct antibiotic inspections. The test cycle is short, the pretreatment method is simple and reliable, the recovery rate is between 75-115%, no special experimental equipment is required, and the test cost is low.

[0108] Through the cooperation of the transverse movement component 3 and the longitudinal movement component 4, each component can be driven to move transversely and longitudinally to meet the processing work requirements;

[0109] The discharge vertical shift assembly 5 cooperates with the powder quantitative mechanism 6 to achieve stable powder injection, the liquid injection vertical shift assembly 8 cooperates with the liquid extraction and discharge mechanism 9 to achieve stable liquid extraction and discharge, the tube body is driven to move by the switching vertical shift assembly and the switching clamping assembly 25, and the tube body cap is screwed in or unscrewed by cooperating with the clamp arm assembly;

[0110] Stable and efficient vortex oscillation is performed by the oscillation mechanism 7, rapid and stable centrifugal stratification is performed by the centrifugal mechanism 10, nitrogen blowing concentration is achieved by the position-adjusting nitrogen blowing mechanism 11, and temperature is adjusted by the temperature-adjusting water bath 12 for easy water bath cooling;

[0111] The tube rack assembly 13 is used to place various empty tubes, tubes filled with intermediate liquids and tubes filled with waste liquids, which is convenient for taking and placing.

[0112] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A QuEChERS processing detection device for detecting antibiotics in aquatic products, comprising a clamping outer box (1), characterized in that: Two transverse moving assemblies (3) are arranged above the clamping outer box (1), and longitudinal moving assemblies (4) are arranged on the transverse moving assemblies (3), and a plurality of material discharging vertical moving assemblies (5) are arranged on the side of one of the longitudinal moving assemblies (4), and a powder material quantitative mechanism (6) is arranged on the material discharging vertical moving assemblies (5), and a switching vertical moving assembly is arranged on one side of the other longitudinal moving assembly (4), and a switching clamping assembly (25) is arranged on the lower end of the switching vertical moving assembly, and a plurality of liquid injection vertical moving assemblies (8) are arranged on the other side of the other longitudinal moving assembly (4), and a liquid extraction and discharge mechanism (9) is arranged on the liquid injection vertical moving assemblies (8), and the interior of the clamping outer box (1) is provided with a plurality of liquid injection vertical moving assemblies (8). Three pipe rack assemblies (13) are provided, and a plurality of clamp arm assemblies are provided on the middle pipe rack assembly (13). An oscillating mechanism (7), a centrifugal mechanism (10), a position-adjusting nitrogen blowing mechanism (11) and a temperature-adjusting water bath (12) are provided inside the clamping outer box (1). The oscillating mechanism (7) and the temperature-adjusting water bath (12) are located on one side of the middle pipe rack assembly (13), and the centrifugal mechanism (10) and the position-adjusting nitrogen blowing mechanism (11) are located on the other side of the middle pipe rack assembly (13). A position-adjusting camera (70) is provided on the front side of the position-adjusting nitrogen blowing mechanism (11), and an electric control screen (2) is provided on the outside of the clamping outer box (1).

2. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 1, characterized in that: The transverse movement assembly (3) includes two sets of slide rail racks (14) and four transverse movement slides. The two sets of slide rail racks (14) are respectively fixed on both sides of the upper end of the clamping outer box (1). The transverse movement slides are respectively slidably arranged on the corresponding slide rail racks (14). Transverse movement motors (15) are fixed on two of the transverse movement slides. A transverse movement gear is fixed on the output shaft of the transverse movement motor (15). The transverse movement gear is meshed with the slide rail racks (14).

3. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 2, characterized in that: The longitudinal shift assembly (4) comprises a longitudinal shift beam (16) and a longitudinal shift slide (19); the longitudinal shift beam (16) is fixed to the upper ends of two transverse shift slides at relative positions; longitudinal shift rails (17) and longitudinal shift racks (18) are fixed to the sides of the longitudinal shift beam (16); the longitudinal shift slide (19) is slidably arranged on the longitudinal shift rails (17); a longitudinal shift motor (21) is fixed to the longitudinal shift slide (19); a longitudinal shift gear is fixed to the output shaft of the longitudinal shift motor (21); and the longitudinal shift gear is meshed with the longitudinal shift rack (18).

4. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 3, characterized in that: The switching vertical shift assembly and the liquid injection vertical shift assembly (8) have the same structure as the material discharging vertical shift assembly (5). The material discharging vertical shift assembly (5) comprises a vertical shift slide (22) and a vertical shift motor (20). The vertical shift slide (22) is fixed on the longitudinal shift slide (19). A vertical shift slide bar (23) is slidably provided inside the vertical shift slide (22). A vertical shift rack (26) is fixed to the side of the vertical shift slide bar (23). A vertical shift gear is fixed to the output shaft of the vertical shift motor (20). The vertical shift gear is meshed with the vertical shift rack (26). A tube plate (24) is fixed to the lower end of the vertical shift slide bar (23) of the material discharging vertical shift assembly (5).

5. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 4, characterized in that: The switching clamping assembly (25) comprises a switching motor (27), the switching motor (27) being fixed to the lower end of the vertical sliding rod (23) of the switching vertical moving assembly, a switching seat (28) being fixed on the output shaft of the switching motor (27), the cross section of the switching seat (28) being an isosceles right triangle, and electric chucks (29) being fixed on both end faces of the switching seat (28), and chuck jaws (30) being provided on the electric chuck (29).

6. A QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 4 or 5, characterized in that: The powder quantitative mechanism (6) comprises a fixed frame (34), the fixed frame (34) is fixed to the upper end of the vertical sliding rod (23) of the material discharge vertical moving assembly (5) at the corresponding position, a discharge hopper (38) is fixed on the fixed frame (34), a bearing seat (35), a feeding hopper (31), a reducer (32) and a reduction motor (33) are fixed to the upper end of the discharge hopper (38), a rotating shaft is arranged inside the bearing seat (35), and an output shaft of the reduction motor (33) is connected to the reducer The input shaft of the reducer (32) is connected in a transmission manner, the output shaft of the reducer (32) is fixedly connected to the upper end of the rotating shaft, a plurality of material shifting racks (36) are fixed on the rotating shaft, the feeding hopper (31) is connected to the discharging hopper (38), the discharging hopper (38) is in a cone shape, a spiral discharging rod (37) is fixed at the lower end of the rotating shaft, the spiral discharging rod (37) extends out of the discharging hopper (38), a material guide pipe is fixed at the lower end of the discharging hopper (38), and the material guide pipe passes through the tube plate (24) at the corresponding position.

7. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 1, characterized in that: The pipe rack assembly (13) includes a bottom plate (39), which is fixed to the bottom of the clamping outer box (1); a pipe rack (41) is fixed to the upper end of the bottom plate (39); the pipe rack (41) is provided with a plurality of pipe holes (40) with different inner diameters; the clamping arm assembly includes a clamping seat (43), which is fixed to the upper end of the bottom plate (39) of the intermediate pipe rack assembly (13); a clamping push rod (42) is fixed to the side of the clamping seat (43); an adjusting gear is fixed to the telescopic end of the clamping push rod (42); two clamping gears (44) are provided for rotation inside the clamping seat (43); the clamping gears (44) are meshed with the adjusting gears; and a clamping arc arm (45) is fixed to the end of the clamping gear (44).

8. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 4 or 5, characterized in that: The pumping and releasing injection mechanism (9) comprises a fixed base (63), the fixed base (63) is fixed to the lower end of the vertical sliding rod (23) of the injection vertical movement assembly (8), an injection motor (62) is fixed to the upper end of the fixed base (63), an injection screw (64) is rotatably arranged inside the fixed base (63), the output shaft of the injection motor (62) is fixedly connected to the upper end of the injection screw (64), a lifting screw seat (67) is slidably arranged on the fixed base (63), the lifting screw seat (67) is transmission-connected to the injection screw (64), a piston rod (68) is fixed to the lifting screw seat (67), an injection piston (66) is fixed to the lower end of the piston rod (68), an injection sleeve (65) is fixed to the lower end of the fixed base (63), and the injection piston (66) is slidably arranged inside the injection sleeve (65).

9. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 1, characterized in that: The oscillating mechanism (7) comprises an oscillating main machine (46), the oscillating main machine (46) is fixed to the bottom of the clamping outer box (1), an oscillating plate (47) is fixed to the oscillating main machine (46), an oscillating base (48) is fixed to the oscillating base (48), and an oscillating bracket (49) is fixed to the upper end of the oscillating base (48); the centrifugal mechanism (10) comprises a centrifugal main machine (50), the centrifugal main machine (50) is fixed to the bottom of the clamping outer box (1), a centrifugal motor is fixed inside the centrifugal main machine (50), a centrifugal placement plate (51) is fixed to the upper end of the output shaft of the centrifugal motor, and a plurality of centrifugal placement tubes (52) evenly distributed around the circumference are arranged on the centrifugal placement plate (51); and a water bath tube is arranged at the upper end of the temperature-adjusting water bath (12).

10. The QuEChERS processing detection device for detecting antibiotics in aquatic products according to claim 1, characterized in that: The positioning nitrogen blowing mechanism (11) comprises a positioning screw (53), a nitrogen blowing main machine (55) and a lifting screw (59), the positioning screw (53) and the lifting screw (59) are fixed at the bottom of the clamping outer box (1), a positioning screw motor (61) is fixed on the positioning screw (53), the output shaft of the positioning screw motor (61) is fixedly connected to the screw end of the positioning screw (53), a positioning slide seat (54) is provided on the positioning screw (53), the nitrogen blowing main machine (55) is slidably arranged at the bottom of the clamping outer box (1), the nitrogen blowing main machine (55) is fixed on the side of the positioning slide seat (54), a plurality of nitrogen blowing placement tubes (56) are provided on the nitrogen blowing main machine (55), and the nitrogen blowing A water bath is arranged inside the main machine (55), the shell of the nitrogen blowing main machine (55) and the water bath are made of transparent materials, a plurality of nitrogen blowing placement tubes (56) are arranged inside the water bath, a lifting screw motor (69) is fixed on the lifting screw (59), the output shaft of the lifting screw motor (69) is fixedly connected to the screw end of the lifting screw (59), a position adjustment slide seat (54) is arranged on the lifting screw (59), a plurality of nitrogen blowing valves (58) are fixed on the position adjustment slide seat (54), the nitrogen blowing valves (58) are connected to an external nitrogen generator, a nitrogen blowing tube (57) is arranged at the lower end of the nitrogen blowing valve (58), and the nitrogen blowing tube (57) is located above the corresponding nitrogen blowing placement tube (56).

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