Full-automatic rapid detection equipment and detection method for multiple types of target objects in food and application

Abstract

The invention relates to full-automatic rapid detection equipment for multiple types of target objects in food, a detection method and application of the full-automatic rapid detection equipment. A shell of the equipment is internally provided with a sample injection control unit which is used for receiving a sample to be detected, inputting information and sending the information into a corresponding channel; the sample processing unit obtains a to-be-detected sample input by a specified channel based on a preset beat, pretreats the to-be-detected sample to obtain a supernatant, pushes out the to-be-detected sample after sampling is completed, obtains the supernatant and a diluent and / or a detection liquid, uniformly mixes the supernatant and the diluent and / or the detection liquid, and adds the sample to the detection card; the detection card control unit is used for placing a detection card which is not added with a sample at a preset position, incubating the detection card which is added with the sample, pushing the incubated detection card to the detection unit, detecting the detection card by the detection unit, and configuring the control unit; the detection method is realized and is applied to index detection of one or more target objects in food. According to the invention, multi-link cooperation in the detection process is realized, the detection process is clearer, the detection data is complete and ordered, the detection efficiency is high, and the detection result is high in credibility and accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of testing or analyzing materials by means of determining the chemical or physical properties of materials, and in particular to a fully automatic rapid detection device, a detection method and an application of multiple types of targets in food. Background Art

[0002] Food safety means that food is non-toxic and harmless, meets the necessary nutritional requirements, and does not cause any acute, subacute or chronic harm to human health. The existing grain, oil and feed quality and safety issues are mainly manifested in the serious contamination of food products such as grain, oil and feed by fungal toxins, pesticide residues, genetically modified substances, heavy metals, or the presence of veterinary drug residues in feed. As a key link in food safety supervision, it is crucial to do a good job in food safety testing, which includes detecting dangerous targets in food or feed.

[0003] As consumers' requirements for food quality and safety are increasing, it is particularly important to quickly and accurately detect harmful target substances that may exist in food. Rapid detection technology is often used when conducting food safety quality testing. It can shorten the detection cycle while ensuring the detection needs of relevant indicators, and can also reduce its capital investment to a certain extent, thereby improving the economic benefits of the enterprise. At present, the rapid detection technologies that are widely used in the field of food safety mainly include time-resolved fluorescence immunochromatography and colloidal gold immunochromatography. The basic principle is to first fix specific antibodies or antigens on a certain zone of nitrocellulose membrane (NC membrane). When the sample is dripped on one end of the dry nitrocellulose membrane, the sample will move forward along the membrane due to capillary action. During the movement, the corresponding antigen or antibody in the sample (depending on whether it is an antibody or an antigen that is fixed) will specifically bind to the antibody or antigen fixed on the membrane. Finally, the corresponding detector is used to read the signal values ​​of the quality control line and the detection line, and the concentration of the target in the sample is calculated according to the corresponding standard curve. This technology requires the correct analysis of the measured target in a short time.

[0004] The food safety testing methods currently used in the market generally have problems such as full manual process, many operation steps, and large manpower investment. Specifically, the full manual testing process includes multiple links such as sample collection, pretreatment, instrument analysis, etc., each of which requires professional personnel to perform delicate operations. Among them, sample pretreatment, as a key link in the testing process, often involves cumbersome steps and requires certain operating skills. Not only is the operating efficiency low, but it is also easy to introduce more errors, thereby affecting the accuracy of the test results. The errors caused by this manual testing will even run through the entire testing process. Furthermore, manual testing methods often require frequent changes in instrument models and supporting test kits when testing multiple indicators, such as mycotoxins, pesticide residues, genetically modified substances, heavy metals, etc., and the sample pretreatment and dilution methods of each indicator are different, which further increases the difficulty, complexity and cost of the testing process. At the same time, in the actual operation process, some indicators contain methanol, acid, alkali and other components in the extract, and the manual testing work scene is more likely to cause personal injury and environmental pollution.

[0005] Therefore, there is an urgent need for a device that can effectively take into account sample pre-processing and integrate multi-index detection. Summary of the invention

[0006] The present invention solves the problems existing in the prior art and provides a fully automatic rapid detection device, a detection method and an application of multiple types of target objects in food.

[0007] The technical solution adopted by the present invention is a fully automatic rapid detection device for multiple types of targets in food, comprising a housing, wherein the housing is provided with: A sample injection control unit, used to receive samples to be tested, input information and send them to the corresponding channel; A sample processing unit is arranged behind the sample injection control unit, and is used to obtain the sample to be detected input from the designated channel based on a preset beat, perform pre-processing and obtain the supernatant, and push out the sample to be detected after the sampling is completed; A sampling and processing unit, arranged in conjunction with the pre-processing unit, is used to obtain the supernatant and the diluent and / or the detection liquid respectively, mix them evenly, and then add the sample to the detection card; A test card control unit, arranged in conjunction with the sampling processing unit, for placing the unloaded test card at a preset position, incubating the loaded test card, and pushing the incubated test card to the test unit; A detection unit, which is arranged behind the detection card control unit and is used to detect the detection card; A control unit is provided to cooperate with the sample injection control unit, the sample processing unit, the sampling processing unit, the detection card control unit and the detection unit.

[0008] Preferably, the injection control unit includes a preparation station and a waiting station; The preparation station is arranged in cooperation with the housing, and an information collection mechanism is arranged in cooperation with the preparation station; A plurality of waiting stations are arranged in parallel in the shell on the side of the preparation station, and any of the waiting stations is arranged in cooperation with the front of the corresponding channel; the preparation station and the waiting station are arranged in cooperation with each other through a first X-axis moving mechanism, and the waiting station is displaced in the corresponding channel through a corresponding first Y-axis moving mechanism.

[0009] Preferably, the sample processing unit comprises a plurality of stirring assemblies arranged in the middle of the channel, a liquid adding assembly in front of all the stirring assemblies and a conveying assembly behind all the stirring assemblies, shielding doors corresponding to the channels are respectively arranged between the stirring assemblies and the liquid adding assemblies and the conveying assemblies, a through door cooperating with the channel is also arranged in front of the shielding door between the stirring assemblies and the liquid adding assemblies, and a second X-axis moving mechanism and a third X-axis moving mechanism are respectively arranged in cooperation with the shielding door and the through door; A first cleaning component and a first Z-axis moving mechanism are provided in conjunction with any of the stirring components; The conveying assembly comprises a fourth X-axis moving mechanism which is arranged between the end of the channel and the sampling processing unit and the waste output plate, and the fourth X-axis moving mechanism is provided with a fork which cooperates with the sample to be detected.

[0010] Preferably, the sampling processing unit comprises a sampling station and a mixing station; a supernatant filtering mechanism is provided in conjunction with the sampling station, and a second Z-axis moving mechanism is provided in conjunction with the supernatant filtering mechanism; A liquid extraction mechanism is provided in cooperation with the sampling station, the mixing station and the detection card control unit, and a second cleaning component and a three-axis moving mechanism are provided in cooperation with the liquid extraction mechanism.

[0011] Preferably, the detection card control unit comprises a plurality of card slots arranged in parallel along the X-axis direction, and one or more detection cards are arranged in any of the card slots along the Z-axis; and all of the card slots are respectively provided with transfer seats and incubation stations along the Y-axis direction; An opening is provided at the bottom of any of the card slots, a first lever is provided to match the opening, a second Y-axis moving mechanism is provided to match the first lever, the transfer seat and the incubation station, and a fifth X-axis moving mechanism is provided to match the second Y-axis moving mechanism; A second lever is provided in cooperation with the transfer seat and the incubation station, a third Y-axis moving mechanism is provided in cooperation with the second lever, and a sixth X-axis moving mechanism is provided in cooperation with the third Y-axis moving mechanism.

[0012] Preferably, a temperature control component and / or a humidity control component is provided in conjunction with the card slot.

[0013] Preferably, the detection unit comprises a fluorescence detection mechanism and / or a colloidal gold detection mechanism arranged in cooperation with the transfer seat.

[0014] Preferably, a detection card exit station is also provided in conjunction with the transfer seat, the second Y-axis moving mechanism and the fifth X-axis moving mechanism.

[0015] A detection method of the fully automatic rapid detection device for multiple types of targets in food, the detection method comprising the following steps: S1 sets the working rhythm based on the sample to be tested; S2 sets the corresponding channel according to the working rhythm; S3 places the sample to be tested into the sample injection control unit, enters the information, obtains the corresponding test plan, and sends it to the corresponding channel to start waiting; S4 Any sample to be tested is added with liquid and stirred according to the working rhythm until a supernatant is obtained; the sample to be tested is moved to the sampling processing unit; S5 According to the working rhythm, the sampling processing unit obtains the supernatant, mixes it evenly with the diluent and / or the detection liquid, and adds the sample to one or more detection cards controlled by the detection card control unit. After the sampling is completed, the sample processing unit pushes out the sample to be tested; S6 The test card control unit pushes the incubated test cards one by one to the test unit for testing, and the test cards that have completed the test are ejected; S7 All test cards have been tested and the test results are obtained.

[0016] An application of the fully automatic rapid detection device for multiple types of targets in food is applied to the detection of one or more target indicators in food; the target objects include but are not limited to mycotoxins, pesticide residues, genetically modified substances, heavy metals, etc.

[0017] The present invention relates to a fully automatic rapid detection device, a detection method and an application of multiple types of target objects in food. A sampling control unit for receiving samples to be detected, inputting information and sending them to corresponding channels is arranged in the shell of the device. A sample processing unit obtains the samples to be detected inputted from a designated channel based on a preset rhythm, performs pretreatment and obtains a supernatant, and pushes out the samples to be detected after sampling is completed. A sampling processing unit obtains the supernatant and a diluent and / or a detection liquid respectively, mixes them evenly and then adds the samples to a detection card. A detection card control unit places a detection card without the samples added at a preset position, incubates the detection card after the samples are added, and pushes the incubated detection card to the detection unit. The detection unit detects the detection card, and a control unit is configured. The detection method is implemented and applied to the detection of one or more target object indicators in food.

[0018] The beneficial effects of the present invention are: (1) Integrate the overall detection and analysis process and achieve multi-link coordination of the detection process through the control of work rhythm; (2) By inputting the information of the samples to be tested, the process of single or batch samples to be tested is automatically controlled. All samples to be tested are in an orderly process, and the whole process of dilution, sampling, adding and testing is controlled, making the testing process clearer; (3) The test data is complete and orderly; (4) It can detect one or more target indicators such as mycotoxins, pesticide residues, genetically modified substances, heavy metals, etc. in single or large batches of samples to be tested. It has high detection efficiency, reduces the proportion of manual operations in the detection process, and has high credibility and accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the top view structure of the present invention with the shell and internal pillars omitted; Figure 2 for Figure 1 On the basis of the above, the outer cover, the temperature and humidity control component and the bottom support plate provided with the card slot are further omitted; Figure 3 For the present invention Figure 2 The main view structure diagram based on the Figure 4 For the present invention Figure 2 The left view structural diagram based on ; Figure 5 For the present invention Figure 2 The right view structural diagram based on the present invention; Figure 6 For the present invention Figure 2 A schematic diagram of a three-dimensional structure based on the Figure 7 It is a structural schematic diagram of the supernatant filtering mechanism in the present invention; Figure 8 It is a schematic diagram of the structure of the transfer seat in the present invention; Fig. 9 It is a schematic diagram of the whole device of the present invention; Fig.10 The present invention is a flow chart of the method. DETAILED DESCRIPTION

[0020] The present invention is further described in detail below in conjunction with embodiments, but the protection scope of the present invention is not limited thereto.

[0021] The present invention is described in detail below. It is agreed that the main working surface is the XOY coordinate plane. Relative to the front of the device, the left and right direction is the X-axis direction, and the front and back direction is the Y-axis direction. The structural setting in the present invention is adjustable and is not limited to the X-axis and Y-axis directions in the embodiment; the direction of movement perpendicular to the XOY coordinate plane is the Z-axis direction.

[0022] The present invention relates to a fully automatic rapid inspection device for multiple types of targets in food, comprising a housing 51, wherein the housing 51 is provided with: A sample injection control unit, used to receive the sample to be tested, input information and send it to the corresponding channel 1; A sample processing unit, which is arranged behind the sample injection control unit, is used to obtain the sample to be detected input from the designated channel 1 based on a preset beat, perform pre-processing and obtain the supernatant, and push out the sample to be detected after the sampling is completed; A sampling processing unit, arranged in conjunction with the pre-processing unit, is used to obtain the supernatant and the diluent and / or the detection liquid respectively, mix them evenly and then add the sample to the detection card 2; A test card control unit, arranged in conjunction with the sampling processing unit, for placing the unsampled test card 2 at a preset position, incubating the sampled test card 2, and pushing the incubated test card 2 to the test unit 39; A detection unit 39, which is arranged behind the detection card control unit and is used to detect the detection card 2; A control unit is provided to cooperate with the sample injection control unit, the sample processing unit, the sampling processing unit, the detection card control unit and the detection unit 39.

[0023] In the present invention, all units of the system are controlled and work in coordination with the control unit. Those skilled in the art can set the control unit according to their needs. The working process is as follows: the sample to be detected is placed on the injection end of the injection control unit. After the injection control unit enters the information, the sample to be detected is sent to the corresponding channel 1 according to the working rhythm. During the implementation process, three channels 1 are used to perform specific detection work; for each sample to be detected that arrives at a specific execution site, the sample processing unit pre-processes the sample to be detected input into the designated channel 1, including but not limited to adding water and stirring once or multiple times until the stirring is preset for a time and / or number of times. After that, the layered samples to be tested are obtained and sent to the sampling station 3, and then the sampling processing unit obtains the supernatant, mixes it with the diluent and / or the test liquid respectively, and then adds samples to the multiple test cards 2 pushed out by the test card control unit one by one, and the sample processing unit pushes out the sample processing unit that has completed sampling; finally, the test card control unit is controlled to incubate the test card 2 after the sample is added, and pushes the incubated test card 2 to the detection unit 39 for detection, where detection of one or more target objects is obtained based on different test liquids and / or test cards 2, and the residues include but are not limited to mycotoxins, pesticide residues, genetically modified substances, heavy metals, etc.

[0024] In the present invention, it is obvious that the outer cover of the working surface and the units thereon (except the card slot 4 of the test card 2 and the entry and exit channel 1 of the sample to be tested) is provided with a shell 51, and necessary internal pillars, partitions, etc. are provided in the shell 51. A control panel and a display screen 52 are provided on the front side of the shell 51 to facilitate the operator to operate and obtain visual results.

[0025] In the present invention, a large number of mechanisms involving X-axis and Y-axis translation and Z-axis vertical movement are generally implemented by a structure in which a motor outputs power to a driving wheel, and the driving wheel drives a driven wheel through a synchronous belt. Those skilled in the art can also implement it by using an existing transmission mechanism, which is a conventional technology in the field.

[0026] The injection control unit includes a preparation station 5 and a waiting station; The preparation station 5 is provided in cooperation with the housing 51, and an information collection mechanism 8 is provided in cooperation with the preparation station 5; A number of waiting stations are arranged in parallel in the shell 51 on the side of the preparation station 5, and any of the waiting stations is arranged in cooperation with the front of the corresponding channel 1; the preparation station 5 and the waiting station are arranged in cooperation with each other through a first X-axis moving mechanism 6, and the waiting station is displaced in the corresponding channel 1 through a corresponding first Y-axis moving mechanism (not shown in the figure).

[0027] In the present invention, the preparation station 5 is openly arranged at the shell 51, and the sample to be tested is placed in the preparation station 5. During the implementation process, the sample to be tested refers to a test cup containing a certain amount of food sample, and the test cup is generally made of PE material to ensure that it is lightweight and has no detection interference; after the sample to be tested is placed, the information collection mechanism 8 enters information about the sample, which can be achieved by scanning the barcode outside the test cup, etc. Correspondingly, a through hole is provided on the side wall of the preparation station 5 to enable the information collection mechanism 8 to collect the barcode, etc., and then the test cup is weighed and peeled, and a sample of appropriate mass is weighed. The control unit automatically calculates the amount of liquid added for the sample to be tested at the liquid adding component 9. After completion, the first X-axis moving mechanism 6 moves the sample to be tested to the corresponding waiting station, which refers to the starting end of one of the three channels 1. A connecting plate 10 is provided between channels 1 and 1. Similarly, a connecting plate 10 is also provided between channels 1 and the ends of channels 1 to facilitate the transfer of the sample to be tested.

[0028] In the present invention, in the specific implementation process, in addition to the structure in which the motor outputs power to the driving wheel and the driving wheel drives the driven wheel through the synchronous belt to realize the X-axis movement, the first X-axis moving mechanism 6 is connected to a push rod 11 on its synchronous belt. An avoidance groove is provided at the place corresponding to the preparation station 5 on the stroke of this push rod 11, that is, it is embedded in the side wall of the preparation station 5 in the initial position. After the push rod 11 is brought out by the synchronous belt, the sample to be tested can be pushed to the starting end of any one of the three channels 1 according to the setting.

[0029] In the present invention, the starting ends of the three channels 1 arranged in parallel correspond to the waiting stations, and at the same time, the conveyor belt corresponding to the waiting station is displaced backward in a step-by-step manner in the corresponding channel 1 (channel 1 is equivalent to the synchronous belt of the first Y-axis moving mechanism) through the first Y-axis moving mechanism, that is, the sample to be detected gradually moves to the corresponding sample processing unit according to a preset time rhythm, and performs subsequent sample processing operations, and channel 1 realizes the sample delivery work in the Y direction through the conveyor belt; the first Y-axis moving mechanism here adopts a synchronous belt structure, and there should be a certain friction between the synchronous belt and the sample to be detected to prevent the sample to be detected from slipping and causing inaccurate movement.

[0030] The sample processing unit includes a plurality of stirring components 12 arranged in the middle of the channel 1, a liquid adding component 9 in front of all the stirring components 12, and a conveying component behind all the stirring components 12. A shielding door 13 corresponding to the channel 1 is respectively arranged between the stirring component 12 and the liquid adding component 9 and the conveying component. A through door 14 cooperating with the liquid adding component 9 is also arranged in front of the shielding door 13 between the stirring component 12 and the liquid adding component 9. A second X-axis moving mechanism 15 and a third X-axis moving mechanism 7 are arranged in cooperation with the shielding door 13 and the through door 14. A first cleaning assembly 16 and a first Z-axis moving mechanism 17 are provided in cooperation with any of the stirring assemblies 12; The conveying assembly includes a fourth X-axis moving mechanism 19 which is arranged between the end of the channel 1 and the sampling processing unit and the waste output plate 18. The fourth X-axis moving mechanism 19 is provided with a fork 20 which cooperates with the sample to be detected.

[0031] In the present invention, the sample processing unit includes adding water to the sample to be tested, stirring until stratification, sending it to the sampling station 3 for sampling, and pushing the sample to be tested together with the test cup to the waste output plate 18 after sampling.

[0032] In the present invention, a liquid adding component 9 is arranged in front of the stirring component 12 in the middle of the channel 1. The liquid adding component 9 mainly includes a pipeline whose liquid output is controlled by the control unit. The pipeline adds the pumped liquid into the detection cup. The liquid output here is based on the information of the sample to be detected collected and entered by the information collection mechanism 8, such as the detection items and the weight of the sample to be detected. The operation of pumping the liquid here is easily understood by those skilled in the art. After the liquid addition is completed, the first Y-axis moving mechanism advances one step to the position of the stirring component 12. The stirring component 12 is easily understood by the public. It mainly includes a stirring paddle and a motor for controlling the stirring of the stirring paddle. After the stirring component 12 completes one or more stirrings, the sample to be detected is advanced by the first Y-axis moving mechanism One or more steps (depending on the working beat and the size of the equipment) to the conveying component, ready to be conveyed to the sampling station 3. During the process, the preceding and succeeding detection cups are not in the space occupied by the conveying component, or at least do not affect the normal progress of the channel, ensuring the feasibility of two-way movement.

[0033] In the present invention, a first cleaning component 16 is provided in conjunction with the stirring component 12, which is used to clean the stirring component 12 that has completed stirring. The cleaning of the first cleaning component 16 can be completed by a vacuum cleaning chamber, which is arranged one by one on each side of the stirring component 12. The stirring component 12 is provided with an X-axis moving mechanism, and the first Z-axis moving mechanism 17 is used to control the up and down movement of the stirring component 12 at the same time. The "up and down" here includes dropping the stirring paddle of the stirring component 12 to a preset position for stirring, and dropping it to a preset position, that is, the corresponding first cleaning component 16 for cleaning; it should be noted that a waste liquid purification mechanism is provided in conjunction with the whole machine, and all waste liquids obtained by cleaning will be discharged after purification.

[0034] In the present invention, in order to ensure that the stirring and liquid adding processes do not affect each other, including liquid splashing, etc., and also to ensure the service life of the whole machine, a shielding door 13 and a through door 14 are set and controlled by the second X-axis moving mechanism 15 and the third X-axis moving mechanism 7 respectively; specifically, at least one set of shielding doors 13 is respectively set between the stirring component 12 and the liquid adding component 9, and between the stirring component 12 and the conveying component. The shielding doors 13 are used to move between the corresponding channel 1 and the avoidance position (such as the vacuum cleaning chamber), playing the role of "opening the door to release, closing the door to block". Generally speaking, the two sets of shielding doors 13 are connected During the stirring process, the two sets of shielding doors 13 are both on the corresponding channels 1 to prevent liquid splashing during the stirring process. After stirring, the two sets of shielding doors 13 are both at the corresponding first cleaning components 16. On the one hand, they also play a role in preventing liquid splashing. On the other hand, the channel 1 is opened to transport the corresponding test cup. The through door 14 is generally one, which is arranged in front of the shielding door 13 between the stirring component 12 and the liquid adding component 9. On the one hand, it is used to prevent liquid splashing at the liquid adding component 9, and on the other hand, it also plays a limiting role of allowing only one test cup to pass through. The through door 14 generally moves synchronously with the liquid adding component 9.

[0035] In the present invention, the conveying assembly is arranged between the sampling processing unit and the waste output plate 18 along the X-axis direction. Before the detection cup to be transferred arrives at the end of the channel 1, it moves to the end of the channel 1 through the fourth X-axis moving mechanism 19. After the detection cup arrives at the end of the channel 1, it directly docks with the fork 20. The fork 20 is connected to the synchronous belt of the fourth X-axis moving mechanism 19. Under the movement of the fork 20, the detection cup is transferred to the sampling station 3 of the sampling processing unit or the top of the waste output plate 18. During the movement of the fork 20, there are no other detection cups at the end of any channel 1; generally, the fork 20 includes a U-shaped groove or a similar groove structure, which can be half-wrapped outside the detection cup; in actual applications, the waste output plate 18 is a downwardly inclined slide, which is convenient for using potential energy to output the detection cup without power.

[0036] The sampling processing unit includes a sampling station 3 and a mixing station 21; a supernatant filtering mechanism is provided in cooperation with the sampling station 3, and a second Z-axis moving mechanism 22 is provided in cooperation with the supernatant filtering mechanism; A liquid extraction mechanism 23 is provided in cooperation with the sampling station 3 , the mixing station 21 and the control unit of the detection card 2 , and a second cleaning component 24 and a three-axis moving mechanism 29 are provided in cooperation with the liquid extraction mechanism 23 .

[0037] In the present invention, the main function of the sampling processing unit is to take the supernatant in the detection cup at the sampling station 3, and mix the supernatant with the diluent and / or detection liquid (placed in the reagent bottle 25) at the mixing station 21, and transfer it to the detection card control unit.

[0038] In the present invention, the supernatant filtering mechanism is directly arranged on the sampling station 3, and its up and down movement is controlled by the second Z-axis moving mechanism 22; as shown in the figure, an embodiment is given, that is, the supernatant filtering mechanism includes a columnar tube 26, and at least one through hole 27 is opened on the columnar tube 26, and a mounting groove is arranged on the outer edge of the through hole 27, which is convenient for installing filter membranes or filter screens 28 with different pore sizes. After placing it in the detection cup, it can filter out the supernatant (the supernatant is in the columnar tube 26) and filter out precipitates of different particle sizes. The top of the columnar tube 26 is open to meet the needs of the liquid extraction mechanism 23 to extract the supernatant.

[0039] In the present invention, generally, the liquid extraction mechanism 23 adopts a pipette needle, which is controlled by a three-axis moving mechanism 29 and can move in a certain three-dimensional space; based on the control of the control unit, the diluent and / or the detection liquid is first transferred to the preset position of the mixing station 21 (mixing well plate), and then the supernatant is extracted at the sampling station 3, and moved to the corresponding preset position of the mixing well plate. After the liquid is injected, it is beaten and mixed until it is evenly mixed, and then transferred to the waiting detection card 2, and then transferred to the second cleaning component 24 for cleaning, completing one transfer; repeat several times until the program preset by the control unit is completed.

[0040] In the present invention, the three-axis moving mechanism 29 includes a coordinated X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, which is easy for those skilled in the art to understand and implement, and those skilled in the art can set it by themselves according to their needs.

[0041] The detection card control unit comprises a plurality of card slots 4 arranged in parallel along the X-axis direction, and one or more detection cards 2 are arranged in any of the card slots 4 along the Z-axis; all the card slots 4 are respectively provided with a transfer seat 30 and an incubation station 31 along the Y-axis direction; An opening 40 is provided at the bottom of any of the card slots 4, a first lever 32 is provided to cooperate with the opening 40, a second Y-axis moving mechanism 33 is provided to cooperate with the first lever 32, the transfer seat 30 and the incubation station 31, and a fifth X-axis moving mechanism 34 is provided to cooperate with the second Y-axis moving mechanism 33; A second lever 35 is provided on the transfer seat 30 and the incubation station 31 , a third Y-axis moving mechanism 36 is provided on the second lever 35 , and a sixth X-axis moving mechanism (not shown) is provided on the third Y-axis moving mechanism 36 .

[0042] A temperature control component and / or a humidity control component 38 is provided in cooperation with the card slot 4 .

[0043] In the present invention, the detection card control unit mainly includes a card slot 4 for placing the detection card 2 and an incubation station 31. A transfer seat 30 is arranged between the card slot 4 and the incubation station 31 for receiving a new detection card 2 and moving it to the incubation station 31, and moving it from the incubation station 31 to the detection unit 39 for detection.

[0044] In the present invention, there are generally multiple card slots 4, which can be used to place test cards 2 corresponding to different test items, or only for storage. The test cards 2 in each card slot 4 are stacked, and the test card 2 at the bottom of the preset card slot 4 is taken out each time until all the cards are taken out. During the process, in fact, in order to facilitate the placement of the test card 2, it is in a relatively open environment and is likely to be exposed to the atmosphere or moisture in the air, resulting in detection failure or inaccuracy. Therefore, an outer cover 41 is provided in conjunction with the card slot 4, and a temperature control component and / or humidity control component 38 is provided in conjunction with the outer cover 41. The outer cover 41 is provided on the bottom support plate 37. Specifically, the temperature control component and / or humidity control component 38 includes a temperature sensor and / or a humidity sensor and a temperature control and / or dehumidification device for ensuring that the temperature and / or humidity are within a preset range. This is content that is easy for technicians in the field to understand, and they can set it according to their needs. The bottom of the card slot 4 passes through the bottom support plate 37 and is connected to the space inside the shell 51.

[0045] In the present invention, the transfer seat 30 is used for transfer and ferrying between the card slot 4 and the incubation station 31 and the corresponding detection unit 39. Considering the overall layout in the housing 51, the operation of entering the detection card 2, transferring the detection card 2 to the incubation station 31, and exiting the detection unit 39 is completed in one direction relative to the transfer seat 30, and the operation of pushing the detection card 2 out of the incubation station 31 and entering the detection unit 39 is completed in another direction relative to the transfer seat 30. Specifically, an opening 40 is provided at the bottom of each card slot 4, and the opening 40 is consistent with the direction in which the detection card is pushed out of the card slot 4. The card slot 4 is generally provided with two parts, that is, the detection card 2 is mounted on the opening 40, and the first lever 32 is driven by the second Y-axis moving mechanism 33 to move, and it moves along the opening 40 from one end of the opening 40 to push the bottom detection card 2 onto the transfer seat 30. During the implementation process, the transfer seat 30 is provided with a structure having a side baffle to ensure that it can be returned to the correct position during the transfer of the detection card 2 to avoid tilting or falling. In order to meet the detection requirements, For the sample introduction and sample withdrawal of the card 2, the transfer seat 30 is generally also provided with left and right halves, and an opening for the first lever 32 to pass through is provided at the bottom, which is consistent with the opening 40 of the card slot 4; then the transfer seat 30 is driven by the fifth X-axis moving mechanism 34 to the preset incubation station 31, generally the incubation stations 31 are multiple in parallel, and incubation begins; it should be noted that the incubation of the incubation station 31 is generally completed by a temperature control device, which is easily understood by those skilled in the art, and the temperature control device can automatically heat up under the control of the control unit, and strictly control the temperature within the preset temperature ±0.5°C; After the incubation is completed, the second lever 35 set at the other section of the incubation station 31 relative to the transfer seat 30 is operated, and the sixth X-axis moving mechanism and the third Y-axis moving mechanism 36 cooperate to drive the second lever 35 to perform X-axis positioning and Y-axis pushing action, and the incubated detection card 2 is pushed out to the waiting transfer seat 30, and then the transfer seat 30 is driven by the fifth X-axis moving mechanism 34 to the detection station at the detection unit 39.

[0046] The detection unit 39 includes a fluorescence detection mechanism and / or a colloidal gold detection mechanism that is arranged in cooperation with the transfer seat 30 .

[0047] In the present invention, in the specific implementation process, the detection unit 39 detects the incubated detection card 2 with a fluorescence detection mechanism and / or a colloidal gold detection mechanism. The fluorescence detection / colloidal gold detection here is a disclosed technical content, and technical personnel in this field can set the structure of the fluorescence detection mechanism and / or the colloidal gold detection mechanism according to their needs.

[0048] In cooperation with the transfer seat 30 , the second Y-axis moving mechanism 33 and the fifth X-axis moving mechanism 34 , a detection card 2 exit station is also provided.

[0049] In the present invention, after the detection is completed, the detection card 2 needs to be withdrawn from the detection unit 39 as soon as possible to avoid affecting the detection work of the next detection card 2. Therefore, the second Y-axis moving mechanism 33 and the fifth X-axis moving mechanism 34 are coordinated, and the first lever 32 acts on the transfer seat 30 to push the detection card 2 to the exit position; in the embodiment, the exit position is located on the other side of the transfer seat 30 relative to the card slot 4, that is, at this time, the first lever 32 can rotate in one direction. Under this setting, the detection card 2 is only movable on one side relative to the transfer seat 30. This is a structure that is easily understood by those skilled in the art. For example, a stopper 44 is used to block the side of the first lever 32 facing the exit position, so that the first lever 32 does not rotate relative to the mounting slot 43 when the detection card 2 is pushed into the transfer seat 30 and pushed out from the transfer seat 30, and the first lever 32 rotates when moving in the reverse direction to avoid the detection card 2; The exit station here is also set to have an inclined slide 42 on one side that is the same height as or slightly lower than the transfer seat 30. The detection card 2 can use the inclined slide 42 to slide out of the shell 51 to the waste collection area (not shown in the figure). The position of the inclined slide 42 can be optimized and adjusted based on the actual structure inside the equipment.

[0050] In the present invention, a full-process tracking device is provided in conjunction with the whole machine, including but not limited to in-place sensors, cameras, etc., which cooperate with the control unit to facilitate real-time acquisition of the actual working status of the whole machine and facilitate backtracking. At the same time, the whole machine is arranged in modules, and each module is equipped with a self-test function. Self-test signals are obtained at a preset frequency and summarized to the control unit. If there is an abnormality in the self-test of any module, an error is reported and the machine enters the maintenance state until the fault is eliminated.

[0051] Furthermore, in the present invention, the device can be connected to the Internet and upload data to the cloud, and at the same time, the usage situation in various places can be checked, and the printing function can be configured based on demand; the device also has self-sleep and scheduled power-on functions. When it is in self-sleep and has not worked for more than a preset time, it will automatically complete the shutdown operation. The scheduled power-on is particularly suitable for environments with lower temperatures. The incubation module can be heated in advance through scheduled power-on to ensure work efficiency.

[0052] The above functions are easily understood by those skilled in the art, and those skilled in the art can configure them according to their needs.

[0053] The present invention also relates to a detection method of the fully automatic rapid detection device for multiple types of targets in food, the detection method comprising the following steps: S1 sets the working rhythm based on the sample to be tested; S2 sets the corresponding channel 1 according to the working rhythm; S3: Place the sample to be tested into the sample injection control unit, enter the information, obtain the corresponding test plan, and send it to the corresponding channel 1 to start waiting; S4 Any sample to be tested is added with liquid and stirred according to the working rhythm until a supernatant is obtained; the sample to be tested is moved to the sampling processing unit; S5 According to the working rhythm, the sampling processing unit obtains the supernatant, mixes it with the diluent and / or the detection liquid, and adds the sample to one or more detection cards 2 controlled by the detection card 2 control unit. After the sampling is completed, the sample processing unit pushes out the sample to be tested; S6 The control unit of the detection card 2 pushes the incubated detection cards 2 one by one to the detection unit 39 for detection, and the detection cards 2 that have completed the detection are withdrawn; S7 All test cards 2 are tested and the test results are obtained.

[0054] The present invention also relates to an application of the fully automatic rapid detection device for multiple types of targets in food, which is applied to the detection of one or more target indicators in food.

[0055] Food here includes but is not limited to grains, feed raw materials, compound feed, fruits and vegetables, tobacco, etc., and even includes coffee and wine; among them, grains need to be tested for fungal toxins, heavy metals, pesticide residues, genetically modified substances, etc., feed raw materials and compound feed need to be tested for fungal toxins and pesticide residues, etc., fruits and vegetables and tobacco often contain pesticide residues, and coffee and wine generally contain ochratoxin to be tested.

[0056] In the present invention, a specific implementation example is given: When the instrument is in "standby" mode, select manual mode, click "add sample", take a 250mL blank sample cup, weigh and select the mode (select conventional sample or highly absorbent sample according to the sample type), weigh 18.0~22.0g of quality control product in a 250mL sample cup, select one or more of the toxins to be tested, vomitoxin, zearalenone, and aflatoxin B1, select the curve and the number of parallels to be tested according to the sample type; the sample enters the instrument, and the extraction solution is added in sequence (according to the sample weight, the extraction is generally performed at a ratio of 1g:5mL). Liquid filling), stirring (stirring for 50 seconds, resting for 10 seconds, three cycles, a total of 3 minutes), standing for 3 minutes, filter pressing (supernatant filtration), dilution (100μL supernatant: 500μL sample diluent, according to the correct proportion according to the requirements of the supporting test card), mixing (pipetting 7 times), sample addition (70μL, according to the instructions for use of the supporting test card), incubation (8 minutes, according to the instructions for use of the supporting test card), reading (according to the test card methodology, select "fluorescence" or "colloidal gold" light source for optical signal detection), and finally output the results; Table 1, Table 2 and Table 3 are the results of the samples tested for vomitoxin, zearalenone and aflatoxin B1 respectively; Table 1 Deoxynivalenol test results

[0057] Table 2 Zearalenone test results

[0058] Table 3 Aflatoxin B1 test results

[0059] The final output results show that the average detection concentration is highly accurate and stable.

[0060] The present invention realizes automation, intelligence and unmanned operation of sample weighing, liquid addition, oscillation extraction, centrifugation, dilution and mixing to detection, and adopts automation and intelligent modules to realize unmanned operation from sample pretreatment to detection; the system is compatible with the current mainstream colloidal gold immunochromatography method and fluorescent immunochromatography method, meets the requirements of industry standards, is fast and accurate, and the detection data can be uploaded to the cloud platform in real time through the 5G function, which is convenient for remote supervision of the detection process and results; the accuracy and repeatability of the detection results are greatly improved, the detection efficiency is greatly improved, and time, labor and cost are saved; the equipment collects and automatically processes waste liquids such as acids, alkalis, and easily-made-drug solvents, which is safer and more environmentally friendly.

[0061] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A fully automatic rapid inspection device for multiple types of targets in food, characterized by: The invention comprises a housing, wherein the housing is provided with: A sample injection control unit, used to receive samples to be tested, input information and send them to the corresponding channel; A sample processing unit is arranged behind the sample injection control unit, and is used to obtain the sample to be detected input from the designated channel based on a preset beat, perform pre-processing and obtain the supernatant, and push out the sample to be detected after the sampling is completed; A sampling and processing unit, arranged in conjunction with the pre-processing unit, is used to obtain the supernatant and the diluent and / or the detection liquid respectively, mix them evenly, and then add the sample to the detection card; A test card control unit, arranged in conjunction with the sampling processing unit, for placing the unloaded test card at a preset position, incubating the loaded test card, and pushing the incubated test card to the test unit; A detection unit, which is arranged behind the detection card control unit and is used to detect the detection card; A control unit is provided to cooperate with the sample injection control unit, the sample processing unit, the sampling processing unit, the detection card control unit and the detection unit.

2. The fully automatic rapid inspection equipment for multiple types of targets in food according to claim 1, characterized in that: The injection control unit includes a preparation station and a waiting station; The preparation station is arranged in cooperation with the housing, and an information collection mechanism is arranged in cooperation with the preparation station; A plurality of waiting stations are arranged in parallel in the shell on the side of the preparation station, and any of the waiting stations is arranged in cooperation with the front of the corresponding channel; the preparation station and the waiting station are arranged in cooperation with each other through a first X-axis moving mechanism, and the waiting station is displaced in the corresponding channel through a corresponding first Y-axis moving mechanism.

3. The fully automatic rapid inspection equipment for multiple types of targets in food according to claim 2, characterized in that: The sample processing unit includes a plurality of stirring components arranged in the middle of the channel, a liquid adding component in front of all the stirring components, and a conveying component behind all the stirring components. A shielding door corresponding to the channel is respectively arranged between the stirring component and the liquid adding component and the conveying component. A through door cooperating with the channel is also arranged in front of the shielding door between the stirring component and the liquid adding component. A second X-axis moving mechanism and a third X-axis moving mechanism are respectively arranged in cooperation with the shielding door and the through door. A first cleaning assembly and a first Z-axis moving mechanism are provided in conjunction with any of the stirring assemblies; The conveying assembly comprises a fourth X-axis moving mechanism which is arranged between the end of the channel and the sampling processing unit and the waste output plate, and the fourth X-axis moving mechanism is provided with a fork which cooperates with the sample to be detected.

4. The fully automatic rapid inspection equipment for multiple types of targets in food according to claim 1, characterized in that: The sampling processing unit includes a sampling station and a mixing station; a supernatant filtering mechanism is provided in conjunction with the sampling station, and a second Z-axis moving mechanism is provided in conjunction with the supernatant filtering mechanism; A liquid extraction mechanism is provided in cooperation with the sampling station, the mixing station and the detection card control unit, and a second cleaning component and a three-axis moving mechanism are provided in cooperation with the liquid extraction mechanism.

5. The fully automatic rapid inspection equipment for multiple types of targets in food according to claim 1, characterized in that: The detection card control unit comprises a plurality of card slots arranged in parallel along the X-axis direction, and one or more detection cards are arranged in any of the card slots along the Z-axis; all the card slots are respectively provided with transfer seats and incubation stations along the Y-axis direction; An opening is provided at the bottom of any of the card slots, a first lever is provided to match the opening, a second Y-axis moving mechanism is provided to match the first lever, the transfer seat and the incubation station, and a fifth X-axis moving mechanism is provided to match the second Y-axis moving mechanism; A second lever is provided in cooperation with the transfer seat and the incubation station, a third Y-axis moving mechanism is provided in cooperation with the second lever, and a sixth X-axis moving mechanism is provided in cooperation with the third Y-axis moving mechanism.

6. The fully automatic rapid inspection device for multiple types of targets in food according to claim 5, characterized in that: A temperature control component and / or a humidity control component is provided in conjunction with the card slot.

7. The fully automatic rapid inspection device for multiple types of targets in food according to claim 5, characterized in that: The detection unit comprises a fluorescence detection mechanism and / or a colloidal gold detection mechanism which are arranged in cooperation with the transfer seat.

8. The fully automatic rapid inspection equipment for multiple types of targets in food according to claim 1, characterized in that: A detection card exit station is also provided in conjunction with the transfer seat, the second Y-axis moving mechanism and the fifth X-axis moving mechanism.

9. A detection method of a fully automatic rapid detection device for multiple types of targets in food according to any one of claims 1 to 8, characterized in that: The detection method comprises the following steps: S1 sets the working rhythm based on the sample to be tested; S2 sets the corresponding channel according to the working rhythm; S3 places the sample to be tested into the sample injection control unit, enters the information, obtains the corresponding test plan, and sends it to the corresponding channel to start waiting; S4 Any sample to be tested is added with liquid and stirred according to the working rhythm until a supernatant is obtained; the sample to be tested is moved to the sampling processing unit; S5 According to the working rhythm, the sampling processing unit obtains the supernatant, mixes it evenly with the diluent and / or the detection liquid, and adds the sample to one or more detection cards controlled by the detection card control unit. After the sampling is completed, the sample processing unit pushes out the sample to be tested; S6 The test card control unit pushes the incubated test cards one by one to the test unit for testing, and the test cards that have completed the test are ejected; S7 All test cards have been tested and the test results are obtained.

10. An application of the fully automatic rapid detection device for multiple types of targets in food according to any one of claims 1 to 8, characterized in that: Applied to the detection of one or more target indicators in food.

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