Residual tuber mustard silage percolate and application thereof in biological feed

Abstract

The invention discloses preserved szechuan pickle residue silage percolate and application thereof in biological feed. The preserved szechuan pickle residue silage percolate is prepared according to the following steps: harvesting and chopping preserved szechuan pickle residues to obtain crushed preserved szechuan pickle residues; adding bacterial enzyme into the crushed leftovers of the preserved szechuan pickles, and uniformly mixing; silage is carried out on the crushed preserved szechuan pickle residues added with the bacterial enzyme; after the ensiling time meets the requirement, collecting ensiling percolate, and adding a preservative into the ensiling percolate. According to the application of the preserved szechuan pickle residue silage percolate in the biological feed, the preserved szechuan pickle residue silage percolate is adopted as an additive of the biological feed. With the adoption of the scheme, the complex organic matters in the preserved szechuan pickle residue silage liquid can be directionally converted, the conversion rate of beneficial components is increased, efficient recovery and utilization of nutritional components and bioactive substances in the percolate are realized, the resource utilization rate is increased, the animal palatability is good, the production cost is low, and large-scale application is facilitated.

Description

Technical Field

[0001] The invention belongs to the technical field of deep processing of mustard tuber tails, and particularly relates to silage exudate of mustard tuber tails and application thereof in biological feed. Background Art

[0002] Zhacai is a product of mustard stems, mainly pickled from its enlarged stem nodules (green cabbage heads). During its production and processing, the treatment of silage liquid from the mustard tails (a byproduct of the mustard processing, mainly referring to the mustard leaves) is particularly important. As a rich green plant resource, the mustard tails are rich in nutrients, including protein, amino acids and dietary fiber. However, these resources are often not fully utilized in traditional processing, and face challenges in tail utilization and environmental pollution.

[0003] Surveys show that the amount of mustard tuber cultivation in Fuling is large, and most of the mustard tuber waste is abandoned in the fields and not fully utilized. Studies have shown that after proper treatment, the silage of mustard tuber waste can not only be used as a supplement to animal feed, but also improve the nutritional value of feed, especially showing certain potential in improving the meat quality of poultry and livestock. However, there are still some challenges in using the silage of mustard tuber waste as feed, including how to ensure the safety of the final feed product; more importantly, how to develop silage of mustard tuber waste with high nutritional value (especially high betaine and total sugar content), good animal palatability and low production cost is the key point and difficulty in promoting the resource utilization of mustard tuber waste. Summary of the invention

[0004] The invention aims to provide a silage exudate of mustard tuber tail and application thereof in biological feed.

[0005] The present invention adopts the following technical solution.

[0006] A mustard tuber tail silage exudate is prepared according to the following steps: Step 1, harvesting and chopping the mustard tuber tails to obtain the mustard tuber tail scraps; Step 2, adding bacterial enzyme to the obtained mustard tuber tail material and mixing them evenly; Step 3, ensilaging the mustard tuber tailings with added bacterial enzymes; Step 4: After the silage time meets the requirements, collect the silage leachate and add a preservative to the silage leachate.

[0007] As a preferred embodiment, the bacterial enzyme is a mixture of 0.8-1.5 parts of Penicillium oxysporum, 10-20 parts of Penicillium oxysporum enzyme and 1500-2500 parts of pure water, and each raw material is calculated by weight.

[0008] As a more preferred embodiment, the bacterial enzyme is a mixture of 1 part of Penicillium oxyphylla, 15 parts of Penicillium oxyphylla enzyme and 2000 parts of pure water, and each raw material is calculated by weight.

[0009] Furthermore, the steps of adding bacterial enzymes to the sauerkraut scraps are as follows: dissolve the weighed amount of Penicillium pyogenes and Penicillin in water, filter and spray them on the sauerkraut scraps, and then add salt or quicklime to the sauerkraut scraps, with the proportion of salt being 0.3% of the total weight of the sauerkraut scraps and the proportion of quicklime being 3% of the total weight of the sauerkraut scraps.

[0010] In the present invention, most of the mustard tuber tail fragments have a size of 10 mm to 20 mm, that is, the volume of the fragments with a size of 10 mm to 20 mm accounts for no less than 85%.

[0011] Furthermore, an application of the aforementioned silage exudate of mustard tuber tails in biological feed, wherein the silage exudate of mustard tuber tails is used as an additive for biological feed.

[0012] Beneficial effects: By adopting the scheme of the present invention, complex organic matter in the silage of mustard tuber tail can be transformed in a targeted manner, the conversion rate of beneficial components is improved, the efficient recovery and utilization of nutrients and bioactive substances in the exudate is achieved, and the resource utilization rate is improved; the silage exudate of mustard tuber tail prepared by the present invention has high betaine and total sugar contents, good animal palatability, low production cost, and is conducive to large-scale application; the present invention also provides a multifunctional biological feed additive, which can reduce the dependence on chemical synthetic feed additives, has excellent effects in nutritional enhancement and functionality improvement, and is conducive to promoting the sustainable development of animal husbandry. DETAILED DESCRIPTION

[0013] The technical scheme of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. In the embodiments, Penicillium oxyphylla and Penicillium oxyphylla enzyme were purchased from Boyide (Beijing) Biotechnology Co., Ltd. Penicillium oxyphylla is a homotypic lactic acid bacterium and a heterotypic lactic acid bacterium prepared in a mass ratio of 1:1, and Penicillium oxyphylla enzyme is a xylanase and a cellulase prepared in a mass ratio of 1:1. Example 1

[0014] A mustard tuber tail silage exudate is prepared according to the following steps: Step 1, harvesting and chopping the mustard tuber tails to obtain the mustard tuber tail scraps; Specifically, the dried mustard tuber tails are evenly fed into the feeding port of the shredder, the machine is started, and the mustard tuber tails are shredded by the shredding cutter, and the shredded mustard tuber tails are discharged through the discharging port, and the mustard tuber tail shreds with a size of 10 mm to 20 mm and a weight of 3 tons are collected; Step 2, adding bacterial enzyme to the obtained mustard tuber tail material and mixing them evenly; Specifically: the weighed amount of Penicillium zymosanus and Penicillin zymosanus enzyme is dissolved in pure water, filtered and evenly sprayed on the mustard tuber scraps, and then salt is added to the mustard tuber scraps and mixed, and the weight of the salt accounts for 0.3% of the total weight (fresh weight) of the mustard tuber scraps; wherein, the amount of Penicillium zymosanus used is 3g, the amount of Penicillin zymosanus enzyme used is 45g, and the amount of pure water used is 6000g (6L); Step 3, ensilaging the mustard tuber tailings with added bacterial enzymes; Specifically: after spraying the silage agent (water-soluble zymosan and zymosan enzyme), immediately put the mustard tuber scraps into the collection bucket and press it tightly, filling it to about 30cm from the bucket mouth, then seal it with plastic film, press it with tap water, and tie it tightly; Step 4, after the ensiling time meets the requirements, collect the silage exudate and add a preservative to the silage exudate; specifically, use a collection bucket (69kg / barrel) to collect the silage liquid during the ensiling process (20% of the exudate will be produced during the ensiling process, about 0.6t of silage liquid will be produced, which is the silage exudate of the mustard tuber tail; add sodium benzoate (the added amount is 0.1% of the total weight of the silage exudate of the mustard tuber tail) to the collected silage exudate of the mustard tuber tail as soon as possible, stir evenly, so that the preservative is evenly distributed in the silage liquid; then store it in a cool, ventilated and dry place, avoid direct sunlight to extend the shelf life, and set the preserved silage liquid aside (69 / L barrel). Example 2

[0015] A silage exudate of mustard tuber tails, referring to Example 1, differs from Example 1 in that: 3 t of mustard tuber tails, 3 g of fermentative bacteria, 45 g of fermentative enzyme, 6000 g (6 L) of pure water are used, and quicklime accounting for 3% of the total weight of the mustard tuber tails is used instead of salt. Example 3

[0016] A silage exudate of mustard tuber tails, which differs from Example 1 in that: 1 t of mustard tuber tails, 1 g of zymolytic bacteria, 15 g of zymolytic enzyme, and 2000 g (2 L) of pure water are used; and the process of adding salt to the mustard tuber tails and mixing them is omitted. Example 4

[0017] A silage exudate of mustard tuber scraps, which differs from Example 1 in that: 4 t of mustard tuber scraps, 4 g of zymox, 60 g of zymox, and 8000 g (8 L) of pure water are used, and the water-soluble zymox and zymox are pulped and then sprayed on the mustard tuber scraps; the step of adding salt to the mustard tuber scraps and mixing them is omitted.

[0018] The silage exudate of the mustard tuber tail in the example was weighed and sampled for testing. The results are shown in Table 1, Table 2 and Table 3. Table 1 Weighing and test results of the silage exudate of mustard tuber tail

[0019] It can be seen that the groups of Examples 1 and 2 performed better in terms of exudate volume, pH value and lactic acid content, indicating that they have a positive effect on the fermentation process of silage exudate and provide a stable source of raw materials for the subsequent development of feed additives.

[0020] The components of the mustard tuber tail (the mustard tuber tail fragments) and the mustard tuber tail silage exudate obtained in some embodiments were tested, including the water content, ash content, protein, organic acid content, vitamin C, amino acids and pesticide residues in the samples. The results are shown in Tables 2, 3 and 4. Table 2 Component test results of mustard tuber tailings (raw materials) Table 3 Detection results of components in the exudate from the mustard tuber silage Table 4 Detection results of pesticide residues in mustard tuber and silage liquid (hygiene index)

[0021] The test results show that the silage exudate of the mustard tuber contains rich nutrients, including vitamin C, crude protein, betaine, etc., especially high betaine and total sugar content, indicating that the silage has high nutritional value. At the same time, the test results of its pesticide residue components meet the safety standards, indicating that the silage is safe as a biological feed additive.

[0022] An application of the silage exudate of mustard tuber tail obtained in Example 1 or Example 2 in biological feed, wherein the silage exudate of mustard tuber tail is used as an additive for biological feed.

[0023] By adopting the scheme of the present invention, complex organic matter in the silage of mustard tuber tail can be transformed in a targeted manner, the conversion rate of beneficial components is improved, the efficient recovery and utilization of nutrients and bioactive substances in the exudate is achieved, and the resource utilization rate is improved; in particular, the silage exudate of mustard tuber tail prepared by the scheme of Example 1 has high betaine and total sugar contents, good animal palatability, low production cost, and is conducive to large-scale application; the present invention also provides a multifunctional biological feed additive, which can reduce the dependence on chemical synthetic feed additives, has excellent effects in nutritional enhancement and functional improvement, and is conducive to promoting the sustainable development of animal husbandry.

Claims

1. A mustard tuber tail silage exudate, characterized in that: It is prepared according to the following steps: Step 1, harvesting and chopping the mustard tuber tails to obtain the mustard tuber tail scraps; Step 2, adding bacterial enzyme to the obtained mustard tuber tail material and mixing them evenly; Step 3, ensilaging the mustard tuber tailings with added bacterial enzymes; Step 4: After the silage time meets the requirements, collect the silage leachate and add a preservative to the silage leachate.

2. The silage exudate of mustard tuber tail according to claim 1, characterized in that: The bacterial enzyme is a mixture of 0.8-1.5 parts of Penicillium oxyphylla, 10-20 parts of Penicillium oxyphylla enzyme and 1500-2500 parts of pure water, and each raw material is calculated by weight.

3. The silage exudate of mustard tuber tail according to claim 1, characterized in that: The bacterial enzyme is a mixture consisting of 1 part of Penicillium oxyphylla, 15 parts of Penicillium oxyphylla enzyme and 2000 parts of pure water, and each raw material is calculated by weight.

4. The silage exudate of mustard tuber tail according to any one of claims 1 to 3, characterized in that: The steps of adding bacterial enzymes to the shavings of mustard tuber are as follows: dissolve the weighed amount of Penicillium pyogenes and Penicillium pyogenes in water, filter and spray them on the shavings of mustard tuber, and then add salt or quicklime to the shavings, with the proportion of salt being 0.3% of the total weight of the shavings, and the proportion of quicklime being 3% of the total weight of the shavings.

5. The silage exudate of mustard tuber tail according to claim 4, characterized in that: Most of the mustard tuber scraps are 10mm~20mm in size.

6. A use of the silage exudate of mustard tuber tails as claimed in any one of claims 1 to 5 in biological feed, characterized in that: The silage exudate of mustard tuber tail is used as an additive for biological feed.

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