Anti-light damage essence added with NAD + composition and preparation process of anti-light damage essence

Abstract

The invention provides an anti-light damage essence added with an NAD + composition and a preparation process thereof, and the essence comprises the following components in parts by weight: 20-30 parts of the NAD + composition, 1-4 parts of butanediol, 0.1-0.5 part of palmitoyl tripeptide-8, 0.1-0.5 part of 1, 3-butanediol, 0.1-0.5 part of 1, 3-butanediol, 0.1-0.5 part of 1, 3-butanediol, 0.1-0.5 part of 1, 3-butanediol, 0.1-0.5 The invention relates to an anti-wrinkle and anti-wrinkle mask, which is prepared from the following raw materials in parts by weight: 1 to 4 parts of 1, 2-hexanediol, 4 to 8 parts of polyethylene glycol-8, 0.1 to 1 part of p-hydroxyacetophenone, 1 to 3 parts of lecithin emulsifying thickener, 0.5 to 2.5 parts of polyacrylate cross-linked polymer-6, 0.01 to 1 part of sodium hyaluronate, 0.1 to 0.8 part of daily essence, 2 to 5 parts of sodium carboxymethyl beta-glucan and 0.1 to 1 part of tetrasodium glutamate diacetate. The prepared essence has the effects of resisting free radicals, resisting oxidation, resisting radiation and resisting light damage, meanwhile, the added NAD + composition can better achieve the effects of resisting aging and tightening skin, can be effectively absorbed by the skin, promotes cell regeneration and differentiation capacity, helps to improve the resilience force of the skin, repair the skin, improve the skin quality and delay skin aging, and has the effects of resisting aging and tightening the skin. And an integrated self-repairing-protection system inside and outside the cells is achieved.

Description

Technical Field

[0001] The present application relates to the field of cosmetic technology, and in particular to an anti-photodamage essence with an added NAD+ composition and a preparation process thereof. Background Art

[0002] Light pollution is a phenomenon in which excessive or inappropriate light radiation produced in modern society has adverse effects on human life and production environment. The damage of ultraviolet rays and blue light to human skin has also attracted more and more attention. Ultraviolet rays and blue light can cause skin cells to produce too much reactive oxygen. The degree of oxidation in the body exceeds the speed of removing oxides, causing an imbalance between the oxidation and antioxidant systems. Excessive reactive oxygen oppresses cells, causing DNA damage, lipid peroxidation, collagen structure damage and mitochondrial function damage. These damages may eventually cause skin problems or skin diseases, such as photoaging, contact dermatitis, atopic dermatitis, skin cancer, seborrheic dermatitis, etc. Plant-derived active ingredients are very common in anti-photodamage cosmetics on the market, but the anti-photodamage effect is average and does not have the anti-aging and firming effects on the skin. Therefore, an anti-photodamage essence with an added NAD+ composition has been developed. Summary of the invention

[0003] The present application provides an anti-photodamage essence with an added NAD+ composition and a preparation process thereof, so as to solve the problem that existing cosmetics have poor anti-photodamage effects, and achieve the effects of anti-free radicals, anti-oxidation, anti-radiation, and anti-photodamage.

[0004] The present application provides an anti-photodamage essence with an added NAD+ composition. The essence components include, by weight, 20-30 parts of the NAD+ composition, 1-4 parts of butylene glycol, 0.1-0.5 parts of palmitoyl tripeptide-8, 1-4 parts of 1,2-hexanediol, 4-8 parts of polyethylene glycol-8, 0.1-1 parts of parahydroxyacetophenone, 1-3 parts of lecithin emulsifier and thickener, 0.5-2.5 parts of polyacrylate cross-linked polymer-6, 0.01-1 parts of sodium hyaluronate, 0.1-0.8 parts of daily fragrance, 2-5 parts of sodium carboxymethyl β-glucan, and 0.1-1 parts of tetrasodium glutamate diacetate.

[0005] Preferably, the essence components include 25 parts of NAD+ composition, 3 parts of butylene glycol, 0.3 parts of palmitoyl tripeptide-8, 3 parts of 1,2-hexanediol, 6 parts of polyethylene glycol-8, 0.6 parts of parahydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, 2 parts of polyacrylate crosslinked polymer-6, 0.4 parts of sodium hyaluronate, 0.5 parts of daily fragrance, 4 parts of sodium carboxymethyl β-glucan, and 0.6 parts of tetrasodium glutamate diacetate.

[0006] Preferably, the components of the NAD+ composition include 40-50 parts of dipropylene glycol, 0.1-1 parts of nicotinamide, 0.001-0.01 parts of nicotinamide adenine dinucleotide, 0.05-0.1 parts of β-nicotinamide mononucleotide, 0.1-0.5 parts of alpine edelweiss flower / leaf extract, 0.1-0.5 parts of alpine edelweiss callus extract, 0.1-0.5 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.1-0.5 parts of citric acid, 0.05-0.1 parts of sodium citrate, 0.1-0.3 parts of sodium benzoate, and 40-60 parts of water.

[0007] Preferably, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Finally, add the NAD+ composition to the third mixed solution, stir and mix evenly at room temperature to obtain the anti-photodamage essence.

[0008] Preferably, in step A, the low-speed stirring rate is 80-180 rpm and the time is 10 min-18 min.

[0009] Preferably, in step B, the high-speed stirring rate is 3000-5000 rpm, and the time is 20 min-40 min.

[0010] Preferably, in step C, the frequency of the ultrasonic reaction is 40-80 kHz, the temperature is 10-20° C., and the time is 2 min-6 min.

[0011] Preferably, the storage temperature in step D is maintained at 20-25°C.

[0012] Beneficial effects: The preparation process of the present invention is simple, and the prepared essence has the effects of anti-free radical, anti-oxidation, anti-radiation, and anti-photodamage. At the same time, the added NAD+ composition can better play the role of skin anti-aging and firming, can be effectively absorbed by the skin, promote cell regeneration and differentiation ability, help improve skin resilience, repair skin, improve skin quality, delay skin aging, and achieve an integrated self-repair-protection system inside and outside the cell; among them, the added palmitoyl tripeptide-8 has antibacterial, antioxidant, immunomodulatory, cholesterol-lowering and other biological activities, and can effectively filter the spectrum within the range of 280nm-500nm; the added polyacrylate cross-linked polymer-6 can form good shielding and achieve good filtering effect. The added carboxymethyl β-glucan sodium and tetrasodium glutamate diacetate have multiple physiological functions such as scavenging free radicals, detoxification, maintaining DNA biosynthesis, normal cell growth and cellular immunity.

[0013] The above description is only an overview of the technical solution of the embodiment of the present application. In order to more clearly understand the technical means of the embodiment of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the embodiment of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions of the embodiments of the present application, a brief introduction will be given below to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0015] Figure 1 This is a diagram of the cell scratch experiment of the present invention. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used in the specification of the application are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification, claims and drawings of this application are intended to cover non-exclusive inclusions.

[0018] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase "embodiments" in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0019] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings.

[0020] The present application provides an anti-photodamage essence with an added NAD+ composition. The essence components include, by weight, 20-30 parts of the NAD+ composition, 1-4 parts of butylene glycol, 0.1-0.5 parts of palmitoyl tripeptide-8, 1-4 parts of 1,2-hexanediol, 4-8 parts of polyethylene glycol-8, 0.1-1 parts of parahydroxyacetophenone, 1-3 parts of lecithin emulsifier and thickener, 0.5-2.5 parts of polyacrylate cross-linked polymer-6, 0.01-1 parts of sodium hyaluronate, 0.1-0.8 parts of daily fragrance, 2-5 parts of sodium carboxymethyl β-glucan, and 0.1-1 parts of tetrasodium glutamate diacetate. Embodiment 1;

[0021] The essence components include 20 parts of NAD+ composition, 1 part of butylene glycol, 0.1 parts of palmitoyl tripeptide-8, 1 part of 1,2-hexanediol, 4 parts of polyethylene glycol-8, 0.1 parts of parahydroxyacetophenone, 1 part of lecithin emulsifier and thickener, 0.5 parts of polyacrylate crosspolymer-6, 0.01 parts of sodium hyaluronate, 0.1 parts of daily fragrance, 2 parts of sodium carboxymethyl β-glucan, and 0.1 parts of tetrasodium glutamate diacetate.

[0022] In this embodiment, the components of the NAD+ composition include 40 parts by weight of dipropylene glycol, 0.1 part of nicotinamide, 0.001 part of nicotinamide adenine dinucleotide, 0.05 part of β-nicotinamide mononucleotide, 0.1 part of alpine edelweiss flower / leaf extract, 0.1 part of alpine edelweiss callus extract, 0.1 part of alpine edelweiss yeast fermentation lysate filtrate, 0.1 part of citric acid, 0.05 part of sodium citrate, 0.1 part of sodium benzoate, and 58 parts of water.

[0023] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0024] In this embodiment, in step A, the low-speed stirring rate is 80 rpm and the time is 10 min.

[0025] In this embodiment, in step B, the high-speed stirring rate is 3000 rpm and the time is 20 min.

[0026] In this embodiment, in step C, the frequency of the ultrasonic reaction is 40 kHz, the temperature is 10° C., and the time is 2 min.

[0027] In this embodiment, the storage temperature in step D is maintained at 20°C. Embodiment 2:

[0028] The essence components include 30 parts of NAD+ composition, 4 parts of butylene glycol, 0.5 parts of palmitoyl tripeptide-8, 4 parts of 1,2-hexanediol, 8 parts of polyethylene glycol-8, 1 part of parahydroxyacetophenone, 3 parts of lecithin emulsifier and thickener, 2.5 parts of polyacrylate crosspolymer-6, 1 part of sodium hyaluronate, 0.8 parts of daily fragrance, 5 parts of sodium carboxymethyl β-glucan, and 1 part of tetrasodium glutamate diacetate.

[0029] In this embodiment, the components of the NAD+ composition include 50 parts of dipropylene glycol, 1 part of nicotinamide, 0.01 parts of nicotinamide adenine dinucleotide, 0.1 parts of β-nicotinamide mononucleotide, 0.5 parts of alpine edelweiss flower / leaf extract, 0.5 parts of alpine edelweiss callus extract, 0.5 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.5 parts of citric acid, 0.1 parts of sodium citrate, 0.3 parts of sodium benzoate, and 46 parts of water.

[0030] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0031] In this embodiment, in step A, the low-speed stirring rate is 180 rpm and the time is 18 min.

[0032] In this embodiment, in step B, the high-speed stirring rate is 5000 rpm and the time is 40 min.

[0033] In this embodiment, in step C, the frequency of the ultrasonic reaction is 80 kHz, the temperature is 20° C., and the time is 6 min.

[0034] In this embodiment, the storage temperature in step D is maintained at 25°C. Embodiment three;

[0035] The essence components include 22 parts of NAD+ composition, 2 parts of butylene glycol, 0.2 parts of palmitoyl tripeptide-8, 2 parts of 1,2-hexanediol, 5 parts of polyethylene glycol-8, 0.2 parts of parahydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, 0.8 parts of polyacrylate crosspolymer-6, 0.04 parts of sodium hyaluronate, 0.2 parts of daily fragrance, 3 parts of sodium carboxymethyl β-glucan, and 0.3 parts of tetrasodium glutamate diacetate.

[0036] In this embodiment, the components of the NAD+ composition include 42 parts of dipropylene glycol, 0.2 parts of nicotinamide, 0.003 parts of nicotinamide adenine dinucleotide, 0.06 parts of β-nicotinamide mononucleotide, 0.2 parts of alpine edelweiss flower / leaf extract, 0.2 parts of alpine edelweiss callus extract, 0.2 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.2 parts of citric acid, 0.06 parts of sodium citrate, 0.2 parts of sodium benzoate, and 56 parts of water.

[0037] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0038] In this embodiment, in step A, the low-speed stirring rate is 90 rpm and the time is 11 min.

[0039] In this embodiment, in step B, the high-speed stirring rate is 3500 rpm and the time is 25 min.

[0040] In this embodiment, in step C, the frequency of the ultrasonic reaction is 45 kHz, the temperature is 12° C., and the time is 3 min.

[0041] In this embodiment, the storage temperature in step D is maintained at 21°C. Embodiment 4:

[0042] The essence components include 28 parts of NAD+ composition, 3 parts of butylene glycol, 0.4 parts of palmitoyl tripeptide-8, 3 parts of 1,2-hexanediol, 7 parts of polyethylene glycol-8, 0.8 parts of parahydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, 2.4 parts of polyacrylate crosspolymer-6, 0.7 parts of sodium hyaluronate, 0.7 parts of daily fragrance, 4 parts of sodium carboxymethyl β-glucan, and 0.8 parts of tetrasodium glutamate diacetate.

[0043] In this embodiment, the components of the NAD+ composition include 48 parts of dipropylene glycol, 0.9 parts of nicotinamide, 0.0091 parts of nicotinamide adenine dinucleotide, 0.09 parts of β-nicotinamide mononucleotide, 0.4 parts of alpine edelweiss flower / leaf extract, 0.4 parts of alpine edelweiss callus extract, 0.4 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.4 parts of citric acid, 0.09 parts of sodium citrate, 0.2 parts of sodium benzoate, and 50 parts of water.

[0044] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0045] In this embodiment, in step A, the low-speed stirring rate is 170 rpm and the time is 17 min.

[0046] In this embodiment, in step B, the high-speed stirring rate is 4500 rpm and the time is 35 min.

[0047] In this embodiment, in step C, the frequency of the ultrasonic reaction is 70 kHz, the temperature is 18° C., and the time is 5 min.

[0048] In this embodiment, the storage temperature in step D is maintained at 24°C. Embodiment five;

[0049] The essence components include 20 parts of NAD+ composition, 4 parts of butylene glycol, 0.1 parts of palmitoyl tripeptide-8, 4 parts of 1,2-hexanediol, 4 parts of polyethylene glycol-8, 1 part of parahydroxyacetophenone, 1 part of lecithin emulsifier and thickener, 2.5 parts of polyacrylate crosspolymer-6, 0.01 parts of sodium hyaluronate, 0.8 parts of daily fragrance, 2 parts of sodium carboxymethyl β-glucan, and 1 part of tetrasodium glutamate diacetate.

[0050] In this embodiment, the components of the NAD+ composition include 43 parts of dipropylene glycol, 0.3 parts of nicotinamide, 0.003 parts of nicotinamide adenine dinucleotide, 0.06 parts of β-nicotinamide mononucleotide, 0.2 parts of alpine edelweiss flower / leaf extract, 0.3 parts of alpine edelweiss callus extract, 0.4 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.2 parts of citric acid, 0.07 parts of sodium citrate, 0.2 parts of sodium benzoate, and 56 parts of water.

[0051] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0052] In this embodiment, in step A, the low-speed stirring rate is 180 rpm and the time is 10 min.

[0053] In this embodiment, in step B, the high-speed stirring rate is 5000 rpm and the time is 20 min.

[0054] In this embodiment, in step C, the frequency of the ultrasonic reaction is 80 kHz, the temperature is 10° C., and the time is 2 min.

[0055] In this embodiment, the storage temperature in step D is maintained at 22°C. Embodiment six;

[0056] The essence components include 25 parts of NAD+ composition, 3 parts of butylene glycol, 0.3 parts of palmitoyl tripeptide-8, 3 parts of 1,2-hexanediol, 6 parts of polyethylene glycol-8, 0.6 parts of parahydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, 2 parts of polyacrylate crosspolymer-6, 0.4 parts of sodium hyaluronate, 0.5 parts of daily fragrance, 4 parts of sodium carboxymethyl β-glucan, and 0.6 parts of tetrasodium glutamate diacetate.

[0057] In this embodiment, the components of the NAD+ composition include 45 parts of dipropylene glycol, 0.5 parts of nicotinamide, 0.005 parts of nicotinamide adenine dinucleotide, 0.07 parts of β-nicotinamide mononucleotide, 0.3 parts of alpine edelweiss flower / leaf extract, 0.3 parts of alpine edelweiss callus extract, 0.3 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.3 parts of citric acid, 0.06 parts of sodium citrate, 0.2 parts of sodium benzoate, and 52 parts of water.

[0058] In this embodiment, a preparation process of an anti-photodamage essence with an added NAD+ composition comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Add the NAD+ composition to the third mixture, stir and mix evenly at room temperature to obtain an anti-aging repairing facial cream.

[0059] In this embodiment, in step A, the low-speed stirring rate is 130 rpm and the time is 14 min.

[0060] In this embodiment, in step B, the high-speed stirring rate is 4000 rpm and the time is 30 min.

[0061] In this embodiment, in step C, the frequency of the ultrasonic reaction is 60 kHz, the temperature is 15° C., and the time is 4 min.

[0062] In this embodiment, the storage temperature in step D is maintained at 24°C.

[0063] Test example: Human epidermal keratinocytes HaCaT in logarithmic growth phase were inoculated in 96-well plates at a density of 2×10 4 cells / mL, with 100 μL per well. The culture system was DMEM high-glucose culture medium supplemented with 10% fetal bovine serum. The inoculated cells were routinely cultured in a carbon dioxide incubator at 37°C and 5% CO 2 for 24 h, irradiated with ultraviolet light and LED blue light, and the relative cell proliferation rate was detected by the WST-1 method.

[0064] In addition, a set of essences was prepared, wherein the essence components by weight included 25 parts of NAD+ composition, 3 parts of butylene glycol, 0.3 parts of palmitoyl tripeptide-8, 3 parts of 1,2-hexanediol, 6 parts of polyethylene glycol-8, 0.6 parts of p-hydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, and polyacrylate cross-linked polymer-6 2 parts, sodium hyaluronate 0.4 parts, daily flavor 0.5 parts, carboxymethyl β-glucan sodium 4 parts, glutamate diacetate tetrasodium 0.6 parts; NAD+ composition components include dipropylene glycol 45 parts, nicotinamide 0.5 parts, nicotinamide adenine dinucleotide 0.005 parts, β-nicotinamide mononucleotide 0.07 parts, citric acid 0.3 parts, sodium citrate 0.06 parts, sodium benzoate 0.2 parts, water 52 parts, using the same experimental conditions as above, and the WST-1 method was used to detect the relative cell survival rate (see Table 1), the OD value was measured at a wavelength of 450nm, cell survival rate %=(sample cells + light OD value / control cell OD value) × 100%, and a cell scratch repair experiment was used to verify the repair effect.

[0065] NAD+ Composition:

[0066] Essence Example:

[0067] The data obtained in Table 1 are as follows:

[0068] It can be seen from the experimental data in the above table that the essence of the present invention can effectively improve the relative cell proliferation rate, thereby better playing the role of skin anti-aging and firming, can be effectively absorbed by the skin, promote cell regeneration and differentiation ability, help improve skin resilience, repair skin, improve skin quality, and delay skin aging. However, the essence without adding alpine edelweiss flower / leaf extract, alpine edelweiss callus extract, and alpine edelweiss yeast fermentation lysate filtrate cannot effectively improve the relative cell proliferation rate, thereby affecting the use effect of the essence.

[0069] In addition, the present invention also carried out a cell scratch experiment. Figure 1 It can be seen that after 18 hours, the repair effect of 1% serum Example 6 is better than that of 1% serum test example and 1% serum group without NAD composition, indicating that the effect of NAD+ compounded with plant extract is better than that of single use of NAD+, and is also better than that of serum without NAD+ composition of the present invention. The compound of NAD+ and plant extract has synergistic repair effect.

[0070] NAD+ is a key component for maintaining cell metabolism and function, and exists in various tissues such as the skin. It is also pointed out that aging can cause a decrease in NAD+ levels, which may affect the skin's ability to repair. The dipropylene glycol added in the present invention has good water, oil and hydrocarbon co-solubility, and has a slight odor, little skin irritation, and low toxicity; the added nicotinamide plays a role in hydrogen transfer in the biological oxidation respiratory chain, which can promote the biological oxidation process and tissue metabolism, and plays an important role in maintaining the integrity of normal tissues; the added nicotinamide adenine dinucleotide is a coenzyme that transfers hydrogen ions, which participates in various physiological activities such as cell material metabolism, energy synthesis, and cell DNA repair; the added β-nicotinamide mononucleotide is one of the key precursors of nicotinamide adenine dinucleotide, which participates in cell It can improve cell energy metabolism and signal transduction, and shows potential application value in anti-aging and improving metabolic syndrome. The added alpine edelweiss flower / leaf extract can gently and deeply cleanse the skin, remove epidermal oil and dirt in the pores, and relieve skin tension at the same time. It is soft and not tight. The natural whitening ingredients can penetrate into the skin, help decompose metabolic impurities, and prevent pigmentation. After use, the skin is smooth, delicate, white and shiny. The added alpine edelweiss callus extract can effectively soothe and calm the skin, and expel harmful substances in the skin. The added alpine edelweiss yeast fermentation lysate filtrate has soothing, calming, whitening and nourishing protective effects on the skin, and has better whitening, hydrating, moisturizing and anti-wrinkle effects.

[0071] In summary, the preparation process of the present invention is simple, and the prepared essence has the effects of anti-free radical, anti-oxidation, anti-radiation, and anti-photodamage. At the same time, the added NAD+ composition can better play the role of skin anti-aging and firming, can be effectively absorbed by the skin, promote cell regeneration and differentiation ability, help improve skin resilience, repair skin, improve skin quality, delay skin aging, and achieve an integrated self-repair-protection system inside and outside the cell; among them, the added palmitoyl tripeptide-8 has antibacterial, antioxidant, immunomodulatory, cholesterol-lowering and other biological activities, and can effectively filter the spectrum within the range of 280nm-500nm; the added polyacrylate cross-linked polymer-6 can form good shielding and achieve good filtering effect, and the added carboxymethyl β-glucan sodium and tetrasodium glutamate diacetate have multiple physiological functions such as scavenging free radicals, detoxification, maintaining DNA biosynthesis, normal cell growth and cellular immunity.

[0072] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it. Although the present application has been described in detail with reference to the aforementioned embodiments, a person of ordinary skill in the art should understand that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features therein can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit of the technical solutions of the embodiments of the present application.

Claims

1. An anti-photodamage essence containing a NAD+ composition, characterized in that: The essence components include 20-30 parts of NAD+ composition, 1-4 parts of butylene glycol, 0.1-0.5 parts of palmitoyl tripeptide-8, 1-4 parts of 1,2-hexanediol, 4-8 parts of polyethylene glycol-8, 0.1-1 parts of parahydroxyacetophenone, 1-3 parts of lecithin emulsifier and thickener, 0.5-2.5 parts of polyacrylate crosslinked polymer-6, 0.01-1 parts of sodium hyaluronate, 0.1-0.8 parts of daily fragrance, 2-5 parts of sodium carboxymethyl β-glucan, and 0.1-1 parts of tetrasodium glutamate diacetate.

2. The anti-photodamage essence containing an NAD+ composition according to claim 1, characterized in that: The essence components include 25 parts of NAD+ composition, 3 parts of butylene glycol, 0.3 parts of palmitoyl tripeptide-8, 3 parts of 1,2-hexanediol, 6 parts of polyethylene glycol-8, 0.6 parts of parahydroxyacetophenone, 2 parts of lecithin emulsifier and thickener, 2 parts of polyacrylate crosspolymer-6, 0.4 parts of sodium hyaluronate, 0.5 parts of daily fragrance, 4 parts of sodium carboxymethyl β-glucan, and 0.6 parts of tetrasodium glutamate diacetate.

3. The anti-photodamage essence containing an NAD+ composition according to claim 1, characterized in that: The NAD+ composition components include 40-50 parts of dipropylene glycol, 0.1-1 parts of nicotinamide, 0.001-0.01 parts of nicotinamide adenine dinucleotide, 0.05-0.1 parts of β-nicotinamide mononucleotide, 0.1-0.5 parts of alpine edelweiss flower / leaf extract, 0.1-0.5 parts of alpine edelweiss callus extract, 0.1-0.5 parts of alpine edelweiss yeast fermentation lysate filtrate, 0.1-0.5 parts of citric acid, 0.05-0.1 parts of sodium citrate, 0.1-0.3 parts of sodium benzoate, and 40-60 parts of water.

4. A process for preparing an anti-photodamage essence with an added NAD+ composition as claimed in claim 1, characterized in that: The preparation process comprises the following steps: A. Mix butylene glycol, palmitoyl tripeptide-8, 1,2-hexanediol, polyethylene glycol-8, p-hydroxyacetophenone, and lecithin emulsifier and thickener, add the mixture into a stirring tank and stir at a low speed to obtain a first mixed solution; B. Mix polyacrylate cross-linked polymer-6, sodium hyaluronate, daily flavor, sodium carboxymethyl β-glucan, and tetrasodium glutamate diacetate, and add the mixture into a stirring tank and stir at high speed to obtain a second mixed solution; C. Mixing the first mixed solution and the second mixed solution, adding the mixture to a reaction tank and performing ultrasonic reaction to obtain a third mixed solution; D. Finally, add the NAD+ composition to the third mixed solution, stir and mix evenly at room temperature to obtain the anti-photodamage essence.

5. The preparation process of the anti-photodamage essence containing NAD+ composition according to claim 4, characterized in that: In the step A, the low-speed stirring rate is 80-180 rpm, and the time is 10 min-18 min.

6. The preparation process of the anti-photodamage essence containing NAD+ composition according to claim 4, characterized in that: In the step B, the high-speed stirring rate is 3000-5000 rpm, and the time is 20 min-40 min.

7. The preparation process of the anti-photodamage essence containing NAD+ composition according to claim 4, characterized in that: In the step C, the frequency of the ultrasonic reaction is 40-80 kHz, the temperature is 10-20° C., and the time is 2 min-6 min.

8. The preparation process of the anti-photodamage essence containing NAD+ composition according to claim 4, characterized in that: In step D, the storage temperature is maintained at 20-25°C.

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