Application of natural plant extract in preparation of medicine for treating inflammatory bowel disease and medicine

Abstract

The invention belongs to the field of natural medicine extracts, and particularly relates to application of a natural plant extract to preparation of a medicine for treating inflammatory bowel disease and the medicine. The natural plant extract is a sedum emarginatum extract, and the sedum emarginatum extract is a water extract or an alcohol extract or a juicing extract or powder. The sedum emarginatum extract has an obvious anti-inflammatory effect on the inflammatory bowel disease; when the sedum emarginatum extract is used as the medicine for treating inflammatory bowel diseases, the treatment cost can be reduced, and the sedum emarginatum extract is effective in treating inflammatory bowel diseases and small in toxic and side effects after being taken for a long time.

Description

Technical Field

[0001] The invention belongs to the technical field of natural drug extracts, and specifically relates to a polyphenol component extracted from Sedumemarginatum Migo, and application of the component in preparing an anti-inflammatory bowel disease drug. Background Art

[0002] Inflammatory bowel disease (IBD) is a chronic nonspecific intestinal inflammatory disease, mainly including ulcerative colitis and Crohn's disease. Clinical manifestations include persistent or recurrent diarrhea, bloody stools with mucus, abdominal pain, and tenesmus, which seriously affect the quality of life of patients. Existing treatments mostly rely on antibiotics and immunosuppressants, but there are problems such as large toxic side effects and easy recurrence. Therefore, finding safe and effective natural medicines has become a research hotspot.

[0003] As a natural herb, Sedum serrata has multiple biological activities such as anti-inflammatory and antioxidant. Modern research shows that its extracts are rich in polyphenol compounds, which have significant effects in anti-oxidation and anti-inflammatory aspects, providing the possibility for the development of new anti-inflammatory bowel disease drugs. Summary of the invention

[0004] The present invention aims to provide a polyphenol component extracted from Sedum truncatum and application of the component in the preparation of an anti-inflammatory bowel disease drug, so as to solve the problems of large toxic and side effects and easy recurrence in existing treatment methods.

[0005] The technical solution of the present invention is as follows: The invention discloses use of a natural plant extract in preparing a medicine for treating inflammatory bowel disease. The natural plant extract is a Sedum serrata extract.

[0006] The Sedum truncatum extract is an alcohol extract of Sedum truncatum or various organic solvent extracts or water extracts or juice extracts or powder.

[0007] The Sedum sedum extract contains polyphenol components.

[0008] The Sedum sedum extract inhibits inflammatory bowel disease by reducing the secretion of at least one of the inflammatory factors IL-1β or TNF-α in intestinal epithelial cells.

[0009] The Sedum sedum extract can treat inflammatory bowel disease by reducing ulcer bleeding, reducing inflammation, relieving pain and resisting oxidation.

[0010] The Sedum sedum extract is dissolved in water, and the crude drug concentration is 0.01-100 g / ml.

[0011] A medicine for treating inflammatory bowel disease, characterized in that it contains an effective dose of Sedum sedum extract.

[0012] The Sedum sedum extract is an alcohol extract or various organic solvent extracts or water extracts or juice extracts or powder.

[0013] Beneficial effects of the present invention: (1) The Sedum truncatum extract provided by the present invention has significant antioxidant and anti-inflammatory effects, can effectively alleviate the clinical symptoms of inflammatory bowel disease, reduce inflammatory reactions, and promote intestinal mucosal repair.

[0014] (2) Compared with existing treatment methods, the drug provided by the present invention has the advantages of less toxic side effects and higher safety, and is suitable for long-term treatment.

[0015] (3) The present invention uses modern separation and purification technology to extract effective ingredients from Sedum truncatum, providing a scientific basis for the development of new anti-inflammatory bowel disease drugs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The experimental procedures for the treatment of IBD with Sedum serrata extract.

[0017] Figure 2 This is a diagram showing the alleviating effect of Sedum conophyllum on IBD model mice.

[0018] Panels AG are weight change ratio, stool characteristics score, DAI score, duration of red blood cells in stool, duration of white blood cells in stool, stool characteristics picture, and perianal bleeding.

[0019] af represent CTRL group, MODEL group, SEA group, SEE group, SEJ group, and 5-ASA group, respectively. Compared with the MODEL group, P**<0.05, P*<0.01 (n=8).

[0020] Figure 3 The anatomical conditions of mice in each group.

[0021] Panels AE represent colon images, colon length, spleen index, colon tissue section HE staining, and tissue scores, respectively. Panels AF represent CTRL group, MODEL group, SEA group, SEE group, SEJ group, and 5-ASA group, respectively. Compared with the MODEL group, P**<0.05, P*<0.01 (n=8). Bar=200μm.

[0022] Figure 4is the level of colon inflammatory factors in mice.

[0023] Panels AC represent TNF-α, IL-1β, and MPO levels, respectively. The horizontal axis represents the group, and the vertical axis represents the concentration of inflammatory factors.

[0024] Compared with the MODEL group, P**<0.05, P*<0.01 (n=8).

[0025] Figure 5 It is the weight change ratio of mice in each group in the toxicity experiment after continuous administration for 14 days.

[0026] The horizontal axis is time, and the vertical axis is the percentage of body weight change. There was no significant difference among the groups (P>0.05) (n=5).

[0027] Figure 6 These are the organ indexes and serum biochemical indicators of mice in each group in the toxicity experiment.

[0028] Panel AF is the organ index, the horizontal axis is the group, and the vertical axis is the index. Panel GL is the serum biochemical index, the horizontal axis is the group, and the vertical axis is the concentration. ALT, alanine aminotransferase. AST, aspartate aminotransferase. BUN, urea nitrogen. CREA, creatinine. TBIL, total bilirubin. UA, uric acid.

[0029] There was no significant difference among the groups (P>0.05), and all were within the normal range (n=5).

[0030] Figure 7 These are the organ tissue sections (HE staining) of mice in each group in the toxicity experiment.

[0031] AF represents heart, liver, spleen, lung, kidney and colon respectively; 1-3 represents CTRL group, 100 g / kg group and 5-ASA group respectively. Bar=100 μm.

[0032] Figure 8 The content of nine polyphenols in the extract. The horizontal axis is the reagents of different extraction methods, and the vertical axis is the compound concentration (μg / g) (n=3). DETAILED DESCRIPTION

[0033] Purpose: To explore the therapeutic effect, toxicity evaluation and content of active ingredient compounds of Sedum conchae on dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) model mice.

[0034] Experimental methods: First, three different methods were used to extract Sedum truncatum, and the extracts were used to treat DSS-induced IBD model mice. The degree of relief of inflammation was analyzed by observing the symptoms, tissue changes, and inflammatory factor levels of the mice. Animal toxicity experiments were used to evaluate the toxicity of Sedum truncatum. Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) technology was used to detect and quantify the chemical components in the Sedum truncatum extract. Experimental results: Our study demonstrated that Sedum truncatum has therapeutic effects on IBD model mice, including analgesia, hemostasis, anti-inflammatory and protection of tissue damage. In quantitative detection, the total polyphenol content of SEE was higher than that of other extraction methods. Among the nine polyphenols, except for caffeic acid and ferulic acid, the rest were found in the highest content in SEE. High doses of Sedum truncatum extract had no obvious toxicity to mice.

[0035] Experimental conclusion: The extract of Sedum truncatum can treat DSS-induced IBD in mice and is safe and non-toxic. This suggests that Sedum truncatum may be an alternative drug for the treatment of IBD and has good application value.

[0036] 1. Materials and Methods 1.1 Materials 1.1.1 Reagents and Materials Dextran sulfate sodium (DSS) (Cat. No.: MB5535, MeilunBio, China), 5-aminosalicylic acid (5-ASA) (Cat. No.: A8231485, Macklin, China), mouse TNF-α, IL-1β enzyme-linked immunosorbent assay (ELISA) kit (Cat. No.: 1217202, 1210122, Dakewe, China), myeloperoxidase (MPO) activity detection kit (Cat. No.: BC5715, Solarbio, China).

[0037] 1.1.2 Instruments and Equipment Induction cooker (RX22-H0107, Midea, China), mill (SMF01, Supor, China), wall breaking machine (L13-Y91S, Joyoung, China), rotary evaporator and cooling water circulation device (RE311 and CF311C, Yamato, Japan), blast drying oven (DHG-9070A, Yiheng, China), full-wavelength microplate reader (Multiskan SkyHigh, Thermo Fisher Scientific, USA), automatic chemiluminescence immunoassay analyzer (AutoLumo A6000, Autobio Diagnostics, China).

[0038] 1.2 Methods 1.2.1 Extraction of components from Sedum sutchuenensis The whole herb of Sedum serrata is sourced from Ningbo, Zhejiang Province, China, and is harvested in summer. We have developed three extraction methods based on the common drug extraction methods of traditional Chinese medicine and previous research: water extraction, alcohol extraction, and juice extraction.

[0039] Water extraction: Take 20g of the whole herb and add pure water to cover it and 2-3cm above the drug. Soak for 20 minutes and then start to boil. Start timing from the time of boiling and boil for 20 minutes on a slow fire. After filtering and collecting the decoction, add pure water again and boil for 15 minutes. Mix the two decoctions and use a rotary evaporator to make the volume 20ml to obtain the water extract of Sedum sedum (SEA) with a crude drug concentration of 1g / ml.

[0040] Alcohol extraction: Take 20g of fresh whole herb and dry it in a forced air drying oven (60℃) and grind it into powder (about 2g), then pass it through a 60-mesh sieve. Add 20ml of 70% ethanol to the powder, place it in an ultrasonic wave at 40℃ for 30min, centrifuge it (14000rpm, 5min), and take the supernatant. Ethanol is evaporated to obtain an alcohol extract, and 20ml of pure water is added to mix and prepare a crude drug concentration of 1g / ml of Sedum sedum alcohol extract (SEE).

[0041] Juice extraction: Take 20g of fresh whole herb and add 200ml of pure water, use a juicer to crush and squeeze the juice of fresh herb. Filter with 200 mesh gauze, and use a rotary evaporator to dilute the obtained medicine to 20ml to obtain Sedum sedum juice (SEJ) with a crude drug concentration of 1g / ml.

[0042] 1.2.2 Animal husbandry All experimental animals used were 6-8 weeks old, 18-22g SPF grade balb / c female mice. They were cultured under experimental conditions of 22-24℃, 50-60% humidity, 12h light and 12h dark alternation. All mice were free to eat and drink freely. The experiment began after one week of acclimatization.

[0043] 1.2.3 Induction and treatment of animal IBD models The mice were given 3% DSS aqueous solution for one week to freely drink water to obtain the IBD model. The drug dose for treatment was based on the human dose recorded in the literature and converted according to the dose used in experimental animals and humans. We concluded that the dose for mice was 10g / kg per day (raw drug).

[0044] 48 mice were randomly assigned to six groups: control group (CTRL), model group (MODEL), SEA treatment group (SEA), SEE treatment group (SEE), SEJ treatment group (SEJ), and mesalazine treatment group (5-ASA). The CTRL group was given pure water, and the other five groups were given 3% DSS aqueous solution and replaced with pure water after one week. The mice were free to eat and drink water. Figure 1 As shown, at the beginning of the experiment, the mice in the SEA, SEE, and SEJ groups were given a crude drug dose of 10 g / kg SEA, 10 g / kg SEE, and 10 g / kg SEJ by gavage every day for 10 days. At the same time, the mice in the 5-ASA group were given 100 mg / kg 5-ASA, while the CTRL and MODEL groups were given an equal amount of pure water by gavage.

[0045] During the experiment, the weight of each mouse was recorded every day. Feces were collected and observed under a microscope using saline smears, and the fecal characteristics, the presence of red blood cells (occult blood) and white blood cells under the microscope, and the presence of macroscopic blood in the stool were recorded. After the treatment, the mice were anesthetized and dissected using isoflurane, and the spleen index and colon length were recorded. The colon tissue of the mice was taken and added to an equal mass of PBS solution, ground into a homogenate, and stored at -80°C. The colon of each group of mice was embedded in paraffin and sectioned, and then the sections were dewaxed and HE stained, and the colon morphology was observed under a microscope.

[0046] The disease activity index (DAI) score and histological score are shown in Tables 1 and 2 [3, 4]. Spleen index = spleen weight (mg) / body weight (g).

[0047] Table 1. DAI scoring criteria

[0048] Table 2. Organization scoring criteria

[0049] 1.2.4 Animal toxicity test In the treatment of colitis animals, the SEE group had the best effect, so the toxicity of the alcohol extract was evaluated. 25 mice of similar weight were randomly divided into 5 groups: normal control group, 10g / kg group, 50g / kg group, 100g / kg group, and 5-ASA group. The three experimental groups were given 10g / kg, 50g / kg and 100g / kg of SEE by gavage, respectively, the 5-ASA group was given 100mg / kg of 5-ASA by gavage, and the control group was given an equal amount of pure water. The treatment lasted for 14 days and the death and weight changes were recorded. Subsequently, the mice were anesthetized with isoflurane and blood was collected from the orbits, and the organs were removed by dissection. The collected whole blood was centrifuged (4000rpm, 15min) to separate the serum. The organ index was calculated and embedded in paraffin, and the sections were observed by HE staining. The organ index was calculated by dividing the organ mass (mg) by the body weight (g); for the colon, a 2cm length of the colon was taken to weigh the mass.

[0050] 1.2.5 ELISA and MPO assays ELISA kits were used to detect the levels of inflammatory factors in mouse colon tissue and cell supernatant. After the specimens (colon homogenate and cell supernatant) were diluted to appropriate concentrations, 100 μl of the specimen was mixed with 50 μl of antibody and incubated at 37°C for 90 min in the antibody-coated plate, the plate was washed, 100 μl of HRP was added and incubated for 30 min, and the plate was washed again. Finally, the color developer TMB was added and incubated for 10 min to terminate the reaction, and the OD value was detected at a wavelength of 450 nm. Standard curves were prepared using inflammatory factor standards with concentrations of 500, 250, 125, 62.5, 31.3 and 15.6 pg / ml to calculate the concentration of inflammatory factors.

[0051] According to the MPO kit procedure, the diluted specimen and reagent were added and heated in a 37°C water bath for 30 min. Subsequently, the reagent was added and heated in a 60°C water bath for 10 min. The absorbance was detected at a wavelength of 460 nm.

[0052] 1.2.6 Serum biochemical test Alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (CREA), total bilirubin (TBIL) and uric acid (UA) in the serum of mice in the toxicity experiment were detected using a fully automatic biochemical analyzer to observe the liver and kidney function of mice.

[0053] 1.2.7 Quantitative detection of components of Sedum serrata: based on UPLC-MS / MS platform Based on previous studies on the components of Sedum concha [5, 6], we selected several compounds with higher content for quantitative analysis, namely caffeic acid, ferulic acid, gallic acid, hesperidin, isoquercetin, isorhamnetin, kaempferol, protocatechuic acid, and quercetin.

[0054] A Shim-pack GISS C18 column was used, with pure water (containing 0.1% formic acid) as mobile phase A and methanol (containing 0.1% formic acid) as mobile phase B. The column temperature was 40°C, and the flow rate was 0.4ml / min or 0.2ml / min. Mass spectrometry analysis was performed in negative ion mode, with an ion source temperature of 550°C, an ion spray voltage of -4500V, a curtain gas of 30pis, a nebulizer gas (GS1) of 55psi, and an auxiliary gas (GS2) of 55psi. Multiple reaction monitoring mode (MRM) was used to acquire data, and the UPLC-MS / MS method for the compounds was established and optimized.

[0055] The sample pretreatment method was protein precipitation. Take 100μl of the extract reagent (SEA, SEE and SEJ), add 100μl 0.1% formic acid, 100μl internal standard solution and 700μl methanol for protein precipitation. After thorough mixing, centrifuge (14000rpm, 10min) and take the supernatant. 600μl of the supernatant was blown dry with nitrogen at 40℃, and 60μl 90% methanol was added to re-dissolve, and shaken at 800rpm for 20min to fully dissolve it. Subsequently, the solution dissolved in the 96-well plate was centrifuged (3850rpm, 5min) to remove the precipitate. Standard curves were drawn using standard solutions with concentrations of 0.01, 0.1, 1, 10, and 100μg / ml, respectively, and the monomer compounds in the Sedum serrata extract were quantitatively detected using the above-established UPLC-MS / MS method. The experiment was repeated three times.

[0056] 1.2.8 Statistical methods Data were analyzed using GraphPad Prism 9.0 software (GraphPad Software, USA) and SPSS 26.0 software (IBM SPSS Statistics, USA). Quantitative data are expressed as " ". One-way analysis of variance (ANOVA) was used for comparison among groups, and the least significant difference (LSD) test was used for pairwise comparison. All statistical tests were two-sided, and P < 0.05 was considered statistically significant.

[0057] 2. Results 2.1 Therapeutic effect of Sedum truncatum on IBD mice During the 10-day treatment, we recorded the weight changes, fecal characteristics, and fecal red blood cell and white blood cell duration of the mice and calculated the DAI score based on them. Figure 2 As shown in A, after 10 days of treatment, the body weight of mice in each group decreased compared with the CTRL group, but the decrease in the MODEL group was the largest, while the decrease in other groups was smaller. The changes in body weight of the SEA, SEE, SEJ and 5-ASA groups were significantly different from those of the MODEL group (P < 0.01). The feces of mice in each group were collected and observed, such as Figure 2 B. Figure 2 F and Figure 2 As shown in G, the stool characteristics of the CTRL group were normal. The stool of the mice in the MODEL group was sticky and shapeless, with increased water content, and symptoms such as loose stools and watery stools. Blood was visible in the stool with a distinct odor. In addition, sticky feces and blood attached to the hair of the mice in the MODEL group were observed around the anus. The stool characteristics of the mice in the SEA, SEE, SEJ and 5-ASA groups were significantly improved, with normal shape and hardness, no blood in the stool visible to the naked eye, and normal hair around the anus. Among them, the stool characteristics of the SEE group were the best. The collected feces were smeared with normal saline, and the duration of red blood cells and white blood cells was observed under a 40x microscope to determine the presence of occult blood in the stool and the degree of inflammation. As shown in Figure 2 D and Figure 2 As shown in E, during the 10-day treatment, the duration of red and white blood cells in the MODEL group was significantly longer than that in the CTRL group (P < 0.01), and longer than that in all treatment groups. However, there was no statistically significant difference in the frequency of white blood cells in the SEJ group compared with the MODEL group (P > 0.05). Among the treatment groups, the duration of red and white blood cells in the feces of mice in the SEE group was the shortest. Therefore, in this experiment, SEE was most effective in alleviating the symptoms of blood in the stool, inflammation, and diarrhea in mice. Based on the recorded weight changes, stool characteristics, and blood in the stool, we calculated the DAI scores of mice in each group, as shown in Figure 2. Figure 2 C. The DAI scores of mice in the MODEL group were significantly increased (P < 0.01), while those in each treatment group were alleviated.

[0058] After the treatment, the mental state and behavior of mice in each group were observed. The mice in the MODEL group showed pain symptoms such as weight loss, persistent arched back, rough hair, slow reaction, and reduced interaction with the group. The mice in the CTRL group and the treatment groups did not have such behavioral changes. The mice were dissected and the colon tissue was removed to observe the morphology, such as Figure 3 A and Figure 3As shown in B, the length of the colon of mice in the CTRL group was normal. The length of the colon of mice in the MODEL group was significantly shortened, which was statistically significant compared with CTRL and each treatment group (P < 0.01). In addition, the colon of mice in the MODEL group showed visible ulcers and bleeding, and had characteristics such as easy breakage, while the colon morphology of mice in each treatment group was significantly improved. The colon tissue was paraffin sectioned and HE stained, and the tissue morphology was observed under a microscope. Figure 3 D. It can be seen that the crypt structure of the CTRL group was normal, without inflammatory cell infiltration; the crypts of the MODEL group were branched, twisted, glandular atrophy, irregular surface, reduced goblet cell mucus, and inflammatory cell infiltration; the SEA, SEE, SEJ and 5-ASA groups had mild inflammatory cell infiltration at the base and normal crypt structure. According to the tissue scoring table, the score of the model group was significantly different from that of the treatment group (P < 0.01). Figure 3 C. The spleens of mice were removed and the spleen index was calculated. The results showed that the spleen index of mice in the MODEL group was significantly higher than that in the other groups, indicating that the systemic inflammatory symptoms in each treatment group were alleviated. Figure 3 E.

[0059] 2.2 Detection of inflammatory factors in mice After the mice in each group in Section 3.1 were dissected, the colon tissues were ground and added to PBS solution, and then diluted to an appropriate multiple for the detection of inflammatory factors. Figure 4 As shown in the figure, ELISA results showed that the colon TNF-α level was significantly increased in the MODEL group (P < 0.05) and decreased after treatment. However, although the colon TNF-α level of mice in the SEA group was reduced, there was no statistical difference in the degree of reduction (P > 0.05). The remaining groups all decreased to the level of the CTRL group. Similarly, the colon IL-1β level was significantly increased in the MODEL group (P < 0.01) and decreased after treatment. Except for the SEA group, the IL-1β levels in other groups were significantly lower than those in the MODEL group (P < 0.01). The colon MPO level also showed a similar trend, P CTRL、5-ASA <0.01, P SEE、SEJ <0.05.

[0060] In summary, SEE has the best symptom relief effect on IBD mice.

[0061] 2.3 Animal toxicity studies No mice died during the 14-day treatment, so the median lethal dose (LD50) of Sedum serrata was greater than 100 g / kg. Figure 5 As shown in the figure, there was no difference in the weight change trend of mice in each group (P>0.05). After the mice were dissected, the organ weights were weighed and the organ index was calculated. Compared with the CTRL group, there was no difference in the organ index levels of each group (P>0.05). Figure 6As shown. In the serum biochemical results of mice, the indicators of each group had no difference with the CTRL group (P>0.05), and were all within the normal range. The organs of the CTRL group, 100g / kg group and 5-ASA group were sliced ​​and observed under a microscope after HE staining. Figure 7 As shown, the microscopic morphology of the heart, liver, spleen, lung, kidney, and intestine in each group was normal, and no lesions were found.

[0062] The above results show that the maximum tolerance dose of SEE for mice is greater than 100g / kg, and the drug at this dose has no obvious liver and kidney toxicity to mice.

[0063] 2.4 Quantitative detection of monomer components of Sedum serrata The liquid chromatography gradient elution time was 5 min. The pumping mode of caffeic acid, ferulic acid, hesperidin, isoquercetin, isorhamnetin, kaempferol and quercetin was binary flow, with a flow rate of 0.4 ml / min, and the elution program was: 0-0.5 min (15% B), 0.5-1.0 min (15-90% B), 1.0-3.5 min (90% B), 3.5 -4.0 min (90-15% B), 4.0-5.0 min (15% B). The pumping mode of gallic acid and protocatechuic acid was isocratic flow, with a flow rate of 0.2 ml / min, and the elution program was 0-5 min (90% B). The mass spectrometry parameters are shown in Table 3. After the UPLC-MS / MS method was established, the internal standard method was used to quantitatively analyze the above compounds, and the number of micrograms of compounds contained in each gram of crude drug was calculated. The results are shown in Table 3. Figure 8 shown.

[0064] Table 3. Mass spectrometry parameters of nine compounds .

Claims

1. Use of a natural plant extract in the preparation of a drug for treating inflammatory bowel disease, wherein the natural plant extract is a Sedum serrata extract.

2. Use of the natural plant extract according to claim 1 in preparing a drug for treating inflammatory bowel disease, characterized in that: The Sedum truncatum extract is an alcohol extract, a water extract, a juice extract or powder of Sedum truncatum.

3. Use of the natural plant extract according to claim 1 or 2 in the preparation of a drug for treating inflammatory bowel disease, characterized in that: The Sedum sedum extract contains polyphenol components.

4. Use of the natural plant extract according to claim 1 in preparing a drug for treating inflammatory bowel disease, characterized in that: The Sedum serrata extract contains caffeic acid, ferulic acid, gallic acid, hesperidin, isoquercetin, isorhamnetin, kaempferol, protocatechuic acid and quercetin.

5. Use of the natural plant extract according to claim 1 in preparing a drug for treating inflammatory bowel disease, characterized in that: The Sedum sedum extract inhibits inflammatory bowel disease by reducing the secretion of at least one of the inflammatory factors IL-1β or TNF-α in intestinal epithelial cells.

6. Use of the natural plant extract according to claim 1 in preparing a drug for treating inflammatory bowel disease, characterized in that: The Sedum sedum extract can treat inflammatory bowel disease by reducing ulcer bleeding, reducing inflammation, relieving pain, and resisting oxidation.

7. Use of the natural plant extract according to claim 1 in preparing a drug for treating inflammatory bowel disease, characterized in that: The Sedum sedum extract is dissolved in water, and the crude drug concentration is 0.01-100 g / ml.

8. A drug for treating inflammatory bowel disease, characterized in that: It contains an effective dose of Sedum sedum extract.

9. The drug for treating inflammatory bowel disease according to claim 8, characterized in that: The Sedum sedum extract is an alcohol extract or various organic solvent extracts or water extracts or juice extracts or powder.

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