Traditional Chinese medicine compound preparation for preventing and treating respiratory tract infection of livestock and poultry as well as preparation method and application of traditional Chinese medicine compound preparation

Abstract

The invention provides a traditional Chinese medicine compound preparation for preventing and treating respiratory tract infection of livestock and poultry and a preparation method and application thereof, and relates to the technical field of animal medicines.The traditional Chinese medicine compound preparation is prepared from honeysuckle, fructus forsythiae, inula flower, platycodon grandiflorum, semen lepidii, cynanchum paniculatum, fresh swamp mahogany, radix stemonae preparata, radix ranunculi ternati and fiveleaf akebia fruit. Through reasonable compatibility, the medicines are mutually coordinated to gain the medicine effect; the traditional Chinese medicine compound preparation disclosed by the invention can be used for remarkably reducing the levels of TNF-alpha and IL-6 in serum and increasing the level of IFN-gamma; clinical symptoms of livestock and poultry respiratory tract infection can be obviously relieved, the death rate is reduced, and a good treatment effect on livestock and poultry respiratory tract infection related diseases is achieved; the drug resistance of antibiotic drugs can be improved, drug residues of chemical agents in livestock and poultry bodies are reduced, and livestock and poultry health and animal-derived food safety are guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of animal medicine, and in particular to a Chinese medicine compound preparation for preventing and treating respiratory tract infection in livestock and poultry, and a preparation method and application thereof. Background Art

[0002] Respiratory diseases in livestock and poultry are caused by the synergistic effects of one or more bacteria, viruses, parasites, chlamydia, environmental factors, etc. It is an acute, highly contagious disease of livestock and poultry, often accompanied by symptoms such as coughing, shaking nose, respiratory rales, wheezing, and dyspnea. Livestock and poultry respiratory infection-related diseases are one of the biggest production problems facing the breeding industry for a long time, posing a serious threat to my country's animal husbandry industry and causing huge economic losses. In addition, some livestock and poultry respiratory infections are zoonotic diseases and pose a threat to public health safety.

[0003] Chinese veterinary medicine has a long history in my country. It can improve the body's own disease resistance and non-specific immune function, and play a good role in preventing and treating respiratory diseases. Traditional Chinese veterinary medicine believes that livestock and poultry respiratory infection-related diseases belong to "epidemic" diseases, which are caused by the immersion of six poisons. The clinical manifestations are rapid onset and large number of cases. The symptoms of the same batch of sick animals are basically similar; the treatment principle is mainly to strengthen the body and eliminate evil, clear away heat and detoxify. At present, veterinary clinics mainly use antibiotics in combination with Western medicines such as antipyretic analgesics and cough suppressants to treat such respiratory diseases. Among them, the antibiotics commonly used to treat livestock and poultry respiratory diseases are mainly tylosin, florfenicol, amoxicillin, ampicillin, doxycycline, etc. However, due to the abuse of antibiotics, the problem of bacterial resistance is becoming more and more serious, resulting in increasingly poor antibiotic treatment effects, and there will be a large amount of drug residues in livestock and poultry, which seriously affects food safety and is directly related to the health of the people. Therefore, it is urgent to develop safe and efficient Chinese veterinary medicine products for prevention and treatment, and they are increasingly valued and concerned by the market. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides a Chinese medicine compound preparation for preventing and treating respiratory tract infections in livestock and poultry, as well as a preparation method and application thereof.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a traditional Chinese medicine compound preparation for preventing and treating respiratory tract infection in livestock and poultry. The traditional Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 40-60 parts of honeysuckle, 40-60 parts of forsythia, 30-50 parts of inula, 30-55 parts of platycodon, 30-50 parts of scutellaria, 20-40 parts of cynanchum wilfordii, 10-40 parts of fresh eucalyptus macrophylla, 10-40 parts of roasted stemonae, 5-25 parts of cat's claw and 5-20 parts of aconite.

[0009] Furthermore, the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 45-55 parts of honeysuckle, 45-50 parts of forsythia, 35-45 parts of inula, 40-50 parts of platycodon, 35-45 parts of scutellaria, 25-35 parts of cynanchum wilfordii, 20-30 parts of fresh eucalyptus, 20-30 parts of roasted stemonae, 10-20 parts of cat's claw, and 8-15 parts of aconite.

[0010] Furthermore, the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 50 parts of honeysuckle, 48 parts of forsythia, 40 parts of inula, 45 parts of platycodon, 40 parts of scutellaria, 28 parts of cynanchum wilfordii, 25 parts of fresh eucalyptus, 22 parts of roasted stemonae, 13 parts of cat's claw, and 10 parts of aconite.

[0011] The present invention provides a method for preparing a Chinese medicinal compound preparation for preventing and treating respiratory tract infection in livestock and poultry, comprising the following steps:

[0012] (1) Weigh the raw materials according to the above weight proportions, wash and crush them, and mix them for later use;

[0013] (2) The crude Chinese medicine powder obtained in step (1) is decocted with water twice, the first decoction is decocted for 2 hours, and the second decoction is decocted for 1 hour, filtered, the decoctions are combined, and concentrated to a relative density of 1.1-1.3.

[0014] Furthermore, the dosage form of the Chinese medicinal compound preparation for preventing and treating respiratory tract infections in livestock and poultry prepared by the preparation method can be granules, oral liquid or injection.

[0015] The present invention provides the use of the traditional Chinese medicine compound preparation in preparing products for preventing and / or treating diseases related to respiratory tract infection in livestock and poultry.

[0016] Pharmacological effects of each Chinese medicine component in the Chinese medicine compound preparation of the present invention:

[0017] Honeysuckle: sweet in taste, cold in nature, enters the lung, heart and stomach meridians; has the effects of clearing away heat and detoxifying, dispersing wind and dissipating heat, and is used for carbuncles, furuncles, throat paralysis, erysipelas, heat-toxic bloody dysentery, wind-heat cold, and fever due to febrile diseases.

[0018] Forsythia suspensa: bitter in taste, slightly cold in nature, enters the lung, heart, and small intestine meridians; has the effects of clearing away heat and detoxifying, reducing swelling and dispersing stagnation, and evacuating wind-heat, and is used for carbuncle, scrofula, breast abscess, erysipelas, wind-heat cold, the initial stage of febrile disease, warm and heat entering the blood, high fever, thirst, coma and rash, and hot and painful stranguria.

[0019] Inula flower: bitter, pungent, and salty in taste, slightly warm in nature, and enters the lung, spleen, stomach, and large intestine meridians; it has the effects of descending qi, eliminating phlegm, promoting water circulation, and stopping vomiting. It is used for wind-cold cough, phlegm accumulation, chest tightness, cough and asthma with excessive phlegm, vomiting and belching, and hardness under the heart.

[0020] Platycodon grandiflorum: bitter and pungent in taste, neutral in nature, enters the lung meridian; has the effects of clearing the lungs, relieving sore throat, removing phlegm, and discharging pus, and is used for cough with excessive phlegm, chest tightness, sore throat and hoarseness, and lung abscess with vomiting pus.

[0021] Trichosanthes kirilowii: pungent and bitter in taste, very cold in nature, enters the lung and bladder meridians; has the effects of clearing the lungs and relieving asthma, promoting water circulation and reducing swelling, and is used for phlegm blocking the lungs, coughing with excessive phlegm, fullness in the chest and ribs, inability to lie flat, edema in the chest and abdomen, and difficulty urinating.

[0022] Xu Changqing: spicy, warm in nature, enters the liver and stomach meridians; has the effects of dispelling wind, removing dampness, relieving pain and relieving itching, and is used for rheumatic pain, stomach pain and bloating, toothache, low back pain, injuries from falls, rubella, and eczema.

[0023] Fresh Eucalyptus globulus: spicy and bitter in taste, neutral in nature, and has the effects of dispelling wind and heat, inhibiting bacteria and inflammation, and preventing and relieving itching. It is used to prevent influenza, epidemic meningitis, treat upper respiratory tract infections, pharyngitis, bronchitis, pneumonia, acute and chronic pyelonephritis, enteritis, dysentery, and filariasis.

[0024] Roasted Stemona: sweet and bitter in taste, slightly warm in nature, and enters the lung meridian; it has the effects of moistening the lungs, lowering qi and relieving cough, killing insects and lice, and is used for new and long-term coughs, coughs caused by tuberculosis, and sudden coughs. It is also used externally for head lice, body lice, pinworm disease, and vaginal itching. Honey Stemona moistens the lungs and relieves coughs, and is used for coughs caused by yin deficiency and fatigue.

[0025] Cat's Claw: sweet and spicy in taste, warm in nature, enters the liver and lung meridians; has the effects of resolving phlegm and dispersing nodules, detoxifying and reducing swelling, and is used for scrofula, phlegm nodules, carbuncle swelling and toxins, and snake and insect bites.

[0026] August Zha: slightly bitter in taste, neutral in nature, enters the liver and stomach meridians; has the effects of soothing the liver and stomach, promoting blood circulation and relieving pain, softening and dispersing lumps; used for liver and stomach qi stagnation, abdominal pain, chest and rib pain, indigestion, diarrhea, hernia pain, low back pain, amenorrhea, dysmenorrhea, goiter and scrofula.

[0027] (III) Beneficial effects

[0028] The invention provides a Chinese medicinal compound preparation for preventing and treating respiratory tract infection in livestock and poultry, as well as a preparation method and application thereof. The Chinese medicinal compound preparation comprises 10 Chinese medicinal materials, namely, honeysuckle, forsythia, inula, platycodon, scutellaria baicalensis, cynomorium longituba, fresh large-leaf eucalyptus, roasted stemonae, cat's claw and aconite, and follows the principle of treatment based on syndrome differentiation in traditional Chinese medicine. The honeysuckle is used as a monarch medicine to exert its effect of clearing away heat and detoxifying; forsythia, inula and platycodon are used as ministerial medicines to assist the monarch medicine and enhance the effects of clearing heat and detoxifying, resolving phlegm and relieving cough; scutellaria baicalensis, cynomorium longituba, fresh large-leaf eucalyptus and roasted stemonae are used as adjuvants to assist in treating symptoms such as wheezing and coughing, edema, rheumatic arthralgia and the like and enhance the effects of clearing heat and detoxifying; cat's claw and aconite are used as guiding medicines to harmonize various medicines, guide the efficacy of the medicines and enhance the effects of soothing the liver and regulating qi.

[0029] The Chinese medicine compound preparation of the present invention can significantly reduce the levels of TNF-α and IL-6 in the serum of diseased livestock and poultry, increase the level of IFN-γ, significantly alleviate the clinical symptoms of respiratory tract infection in livestock and poultry, and reduce the mortality rate; it has a good therapeutic effect on infectious bronchitis, Newcastle disease virus, avian influenza virus, mycoplasma infection, Escherichia coli infection and other livestock and poultry respiratory tract infection-related diseases; it can also improve the drug resistance of antibiotics, reduce the drug residues of chemical agents in livestock and poultry, and ensure the health of livestock and poultry and the safety of animal-derived food. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] A traditional Chinese medicine compound preparation for preventing and treating respiratory tract infections in livestock and poultry, the traditional Chinese medicine compound preparation being composed of the following raw materials in parts by weight: 50 parts of honeysuckle, 48 parts of forsythia, 40 parts of inula, 45 parts of platycodon, 40 parts of scutellaria, 28 parts of cynanchum wilfordii, 25 parts of fresh eucalyptus macrophylla, 22 parts of roasted stemonae, 13 parts of cat's claw and 10 parts of aconite.

[0033] The preparation method of the Chinese medicine compound preparation comprises the following steps:

[0034] (1) Weigh the raw materials according to the above weight proportions, wash and crush them, and mix them for later use;

[0035] (2) boiling the crude Chinese medicine powder obtained in step (1) with water for two times, the first time for 2 hours and the second time for 1 hour, filtering, combining the decoctions, and concentrating to a relative density of 1.1-1.3;

[0036] (3) The concentrated extract obtained in step (2) is mixed with starch in a ratio of 1:1, moistened with 70% ethanol, made into a soft material, granulated, and dried at low temperature to obtain the Chinese medicine compound preparation of the present invention.

[0037] Example 2

[0038] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 40 parts of honeysuckle, 40 parts of forsythia, 30 parts of inula, 30 parts of platycodon, 30 parts of scutellaria, 20 parts of cynanchum wilfordii, 10 parts of fresh eucalyptus, 10 parts of roasted stemonae, 7 parts of cat's claw, and 5 parts of aconite.

[0039] Example 3

[0040] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 45 parts of honeysuckle, 45 parts of forsythia, 38 parts of inula, 42 parts of platycodon, 38 parts of scutellaria, 27 parts of cynanchum wilfordii, 15 parts of fresh eucalyptus, 15 parts of roasted stemonae, 9 parts of cat's claw, and 6 parts of aconite.

[0041] Example 4

[0042] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 60 parts of honeysuckle, 60 parts of forsythia, 50 parts of inula, 55 parts of platycodon, 50 parts of scutellaria, 40 parts of cynanchum wilfordii, 40 parts of fresh eucalyptus, 40 parts of roasted stemonae, 25 parts of cat's claw, and 20 parts of aconite.

[0043] Example 5

[0044] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 52 parts of honeysuckle, 50 parts of forsythia, 43 parts of inula, 48 parts of platycodon, 43 parts of scutellaria, 32 parts of cynanchum wilfordii, 28 parts of fresh eucalyptus, 28 parts of roasted stemonae, 18 parts of cat's claw, and 12 parts of aconite.

[0045] Example 6

[0046] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 58 parts of honeysuckle, 55 parts of forsythia, 47 parts of inula, 52 parts of platycodon, 48 parts of scutellaria, 37 parts of cynanchum wilfordii, 33 parts of fresh eucalyptus, 33 parts of roasted stemonae, 21 parts of cat's claw, and 15 parts of aconite.

[0047] Example 7

[0048] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 43 parts of honeysuckle, 41 parts of forsythia, 31 parts of inula, 33 parts of platycodon, 31 parts of scutellaria, 22 parts of cynanchum, 13 parts of fresh eucalyptus, 13 parts of roasted stemona, 8 parts of cat's claw, and 8 parts of aconite.

[0049] Example 8

[0050] The difference between this embodiment and embodiment 1 is that the Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 55 parts of honeysuckle, 52 parts of forsythia, 45 parts of inula, 50 parts of platycodon, 45 parts of scutellaria, 35 parts of cynanchum wilfordii, 30 parts of fresh eucalyptus, 30 parts of roasted stemona, 20 parts of cat's claw, and 13 parts of aconite.

[0051] Test Example 1

[0052] 1 Materials

[0053] 1.1 Trial Drugs

[0054] Florfenicol soluble powder;

[0055] The Chinese medicine compound preparation is prepared according to Example 1 of the present invention.

[0056] 1.2 Experimental animals

[0057] 250 chickens suffered from respiratory infection caused by Escherichia coli, with clinical manifestations of dyspnea, coughing, listlessness, mucus leakage from the mouth, and diarrhea.

[0058] 2 Methods

[0059] 2.1 Grouping and Dosing

[0060] 250 chickens with respiratory tract infection caused by Escherichia coli infection were randomly divided into 5 groups, each group had 50 chickens, namely a blank control group, a low-dose Chinese medicine compound preparation group, a medium-dose Chinese medicine compound preparation group, a high-dose Chinese medicine compound preparation group, and a florfenicol group; wherein, the blank control group was not given any medicine, the low-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 100g mixed with 500kg of feed, the medium-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 200g mixed with 500kg of feed, the high-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 300g mixed with 500kg of feed, and the florfenicol group was given florfenicol soluble powder, mixed with drink, 150mg per 1L of water; the medicine was given continuously for 7 days, and the other feeding methods and environments were kept consistent, and the experimental chickens were clinically observed every day to record their mental state, cough, death, etc.

[0061] 2.2 Criteria for determining efficacy

[0062] Ineffective: There is no effect or the effect is not obvious during the administration and observation period.

[0063] Effective: Various clinical symptoms were relieved during the medication and observation period, respiratory symptoms were significantly improved, and appetite and mental state improved.

[0064] Cure: All clinical symptoms disappear, breathing returns to normal, appetite and mental state are good, and there is no recurrence after stopping the medication for one week.

[0065] 2.3 Effects of each group of drugs on inflammatory factors in the serum of chickens with respiratory tract infection

[0066] Five sick chickens were randomly selected from each group for cardiac blood sampling after treatment. The blood was placed in a 2-8°C refrigerator for 1-2 hours and centrifuged at 3500 rpm for 15 minutes to separate the serum. The levels of TNF-α, IL-6, and IFN-γ in the serum were detected by ELISA.

[0067] 2.4 Statistical methods

[0068] GraphPad Prism 8.0 statistical analysis software was used for processing. ± standard deviation (s), and the comparison among groups was analyzed by one-way analysis of variance. Bonferroni test was used for analysis when variance was equal, and Dunnett's T3 test was used for analysis when variance was unequal. The test level was α = 0.05.

[0069] 3 Results

[0070] 3.1 Therapeutic effects of each group of drugs on chickens with respiratory tract infection

[0071] Table 1 shows the therapeutic effects of each group of drugs on chickens with respiratory tract infection caused by Escherichia coli infection. The self-healing rate of the blank control group was 40%. Compared with the blank control group, the low, medium and high dose groups of the Chinese herbal compound preparation all had different degrees of therapeutic effects on chickens with respiratory tract infection caused by Escherichia coli, and the mortality rate was significantly reduced; compared with the florfenicol group, the cure rate of the high dose group of the Chinese herbal compound preparation was significantly increased, reaching 78%, indicating that the Chinese herbal compound preparation of the present invention has a good therapeutic effect on chickens with respiratory tract infection caused by Escherichia coli.

[0072] Table 1 The therapeutic effect of each group of drugs on chickens with respiratory tract infection

[0073]

[0074] 3.2 Effects of each group of drugs on inflammatory factors in the serum of chickens with respiratory tract infection

[0075] As shown in Table 2, compared with the blank control group, the low-, medium- and high-dose groups of the Chinese herbal compound could significantly reduce the levels of inflammatory factors TNF-α and IL-6 in the serum of sick chickens, and increase the level of IFN-γ (P<0.01). The effect of the medium-dose group of the Chinese herbal compound was equivalent to that of the florfenicol group, and the effect of the high-dose group of the Chinese herbal compound was significantly better than that of the florfenicol group.

[0076] Table 2 Effects of each group of drugs on inflammatory factors in the serum of chickens with respiratory tract infection

[0077]

[0078] Compared with the blank control group, * P<0.05, ** P<0.01.

[0079] Test Example 2

[0080] 1 Materials

[0081] 1.1 Trial Drugs

[0082] Tiamulin fumarate soluble powder;

[0083] The Chinese medicine compound preparation is prepared according to Example 1 of the present invention.

[0084] 1.2 Experimental animals

[0085] 100 pigs naturally infected with Mycoplasma pneumonia showed clinical manifestations of depression, loss of appetite, fever, difficulty breathing, abdominal breathing, coughing, wheezing, and runny nose.

[0086] 2 Test methods

[0087] 2.1 Grouping and dosing of experimental pigs

[0088] 100 sick pigs naturally infected with mycoplasma pneumonia were randomly divided into 5 groups, 20 in each group, namely blank control group, low-dose Chinese medicine compound preparation group, medium-dose Chinese medicine compound preparation group, high-dose Chinese medicine compound preparation group, and tiamulin fumarate group; wherein, the blank control group was not given any medicine, the low-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 100g mixed with 500kg of feed, the medium-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 200g mixed with 500kg of feed, the high-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 300g mixed with 500kg of feed, and the tiamulin fumarate group was given tiamulin fumarate soluble powder, mixed with drink, 50 mg per 1L of water; the medicine was given continuously for 7 days, and the other feeding methods and environment remained the same, clinical observation of the experimental pigs was carried out every day, and their mental state, cough, death, etc. were recorded.

[0089] 2.2 Clinical efficacy criteria

[0090] Ineffective: There is no effect or the effect is not obvious during the administration and observation period.

[0091] Effective: Various clinical symptoms were relieved during the medication and observation period, respiratory symptoms were significantly improved, and appetite and mental state improved.

[0092] Cure: All clinical symptoms disappear, breathing returns to normal, appetite and mental state are good, and there is no recurrence after stopping the medication for one week.

[0093] 3. The therapeutic effects of each drug on pigs with mycoplasma pneumonia

[0094] As shown in Table 3, the self-healing rate of sick pigs in the blank control group was 30%. Compared with the blank control group, the low, medium and high dose groups of the Chinese herbal compound preparation had different degrees of therapeutic effects on sick pigs, and the mortality rate was significantly reduced; compared with the tylosin fumarate group, the cure rate of the high dose group of the Chinese herbal compound preparation was significantly improved, reaching 76.7%, indicating that the Chinese herbal compound preparation of the present invention has a good therapeutic effect on pigs with mycoplasma pneumonia.

[0095] Table 3 The therapeutic effect of each group of drugs on pigs with mycoplasma pneumonia

[0096]

[0097]

[0098] Test Example 3

[0099] 1 Materials

[0100] 1.1 Trial Drugs

[0101] Tylosin tartrate soluble powder;

[0102] The Chinese medicine compound preparation is prepared according to Example 1 of the present invention.

[0103] 1.2 Experimental animals

[0104] 250 chickens naturally infected with Mycoplasma gallisepticum showed clinical symptoms such as difficulty breathing, coughing, mental depression, mucus leakage from the mouth, and diarrhea.

[0105] 2 Test methods

[0106] 2.1 Grouping and dosing of experimental chickens

[0107] 250 sick chickens naturally infected with Mycoplasma gallisepticum were randomly divided into 5 groups, each group had 50 chickens, namely a blank control group, a low-dose Chinese medicine compound preparation group, a medium-dose Chinese medicine compound preparation group, a high-dose Chinese medicine compound preparation group, and a tylosin tartrate group; wherein, the blank control group was not given any medicine, the low-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 500kg of feed mixed with every 100g, the medium-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 500kg of feed mixed with every 200g, the high-dose Chinese medicine compound preparation group was given the Chinese medicine compound preparation of the present invention, the dosage of which was 500kg of feed mixed with every 300g, and the tylosin tartrate group was given tylosin tartrate soluble powder, mixed with drink, 500mg per 1L of water; the medicine was given continuously for 7 days; the rest of the feeding methods and environment remained the same, clinical observation of the experimental chickens was carried out every day, and their mental state, cough, death, etc. were recorded.

[0108] 2.2 Clinical efficacy criteria

[0109] Ineffective: There is no effect or the effect is not obvious during the administration and observation period.

[0110] Effective: Various clinical symptoms were relieved during the medication and observation period, respiratory symptoms were significantly improved, and appetite and mental state improved.

[0111] Cure: All clinical symptoms disappear, breathing returns to normal, appetite and mental state are good, and there is no recurrence after stopping the medication for one week.

[0112] 3. The therapeutic effects of each drug on chickens infected with Mycoplasma gallisepticum

[0113] As shown in Table 4, the self-healing rate of the diseased chickens in the blank control group was 44.0%. Although the mortality rate was not high, the clinical symptoms were eliminated slowly. Compared with the blank control group, the low, medium and high dose groups of the Chinese herbal compound preparation had different degrees of therapeutic effects on Mycoplasma gallisepticum, and the mortality rate was significantly reduced, and a dose-dependent effect was presented; compared with the tylosin tartrate group, the cure rate of the high dose group of the Chinese herbal compound preparation was significantly improved, which could reach 84%, indicating that the Chinese herbal compound preparation of the present invention has a good therapeutic effect on chickens infected with Mycoplasma gallisepticum.

[0114] Table 4 The therapeutic effect of each group of drugs on chickens infected with Mycoplasma gallisepticum

[0115]

[0116] In summary, the Chinese medicine compound preparation of the present invention can significantly reduce the levels of TNF-α and IL-6 in serum and increase the level of IFN-γ; it can significantly alleviate the clinical symptoms of respiratory tract infection in livestock and poultry, reduce mortality, improve drug resistance caused by long-term use of antibiotics, reduce drug residues of chemical agents in livestock and poultry, ensure the health of livestock and poultry and the safety of animal-derived food, and has good application prospects.

[0117] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicinal compound preparation for preventing and treating respiratory tract infections in livestock and poultry, characterized in that: The traditional Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 40-60 parts of honeysuckle, 40-60 parts of forsythia, 30-50 parts of inula, 30-55 parts of platycodon, 30-50 parts of scutellaria, 20-40 parts of cynanchum wilfordii, 10-40 parts of fresh eucalyptus, 10-40 parts of roasted stemonae, 5-25 parts of cat's claw and 5-20 parts of aconite.

2. A Chinese medicinal compound preparation for preventing and treating respiratory tract infections in livestock and poultry according to claim 1, characterized in that: The traditional Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 45-55 parts of honeysuckle, 45-50 parts of forsythia, 35-45 parts of inula, 40-50 parts of platycodon, 35-45 parts of scutellaria, 25-35 parts of cynanchum wilfordii, 20-30 parts of fresh eucalyptus, 20-30 parts of roasted stemonae, 10-20 parts of cat's claw and 8-15 parts of aconite.

3. A Chinese medicinal compound preparation for preventing and treating respiratory tract infections in livestock and poultry according to claim 1, characterized in that: The traditional Chinese medicine compound preparation is composed of the following raw materials in parts by weight: 50 parts of honeysuckle, 48 parts of forsythia, 40 parts of inula, 45 parts of platycodon, 40 parts of scutellaria, 28 parts of cynanchum wilfordii, 25 parts of fresh eucalyptus, 22 parts of roasted stemonae, 13 parts of cat's claw and 10 parts of aconite.

4. A method for preparing a Chinese medicinal compound preparation for preventing and treating respiratory tract infections in livestock and poultry according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Weighing the raw materials according to the above weight proportions, washing, crushing and mixing them for later use; (2) Adding water to the coarse Chinese medicine powder obtained in step (1) and decocting it twice, the first decoction is 2 hours, and the second decoction is 1 hour, filtering, combining the decoctions, and concentrating them to a relative density of 1.1-1.

3.

5. The method for preparing a Chinese medicinal compound for preventing and treating respiratory tract infections in livestock and poultry according to claim 4, characterized in that: The Chinese medicine compound preparation can be prepared into granules, oral liquid or injection, etc.

6. Use of the Chinese medicinal compound preparation according to any one of claims 1 to 5 in the preparation of products for preventing and / or treating diseases related to respiratory tract infections in livestock and poultry.

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