Rice nutrient solution and application of rice nutrient solution in improving quality of Mushu Proso of Xiangzhao glutinous rice variety

Abstract

The invention discloses a rice nutrient solution and application of the rice nutrient solution to improvement of the quality of Mushu Province of a fragrant grain glutinous rice variety, and relates to the technical field of rice cultivation. The rice nutrient solution provided by the invention is specially used for Matrimony Province of the variety of Xiangzhao glutinous rice, and the formula consists of a basic nutrient solution, phenylalanine and / or salicylic acid and water; when the rice nutrient solution is used for culturing rice seedlings in a three-leaf stage until a five-leaf stage, the biomass, the plant height, the nitrogen content and the chlorophyll content of the plants are remarkably increased, and the yield is greatly increased; the adaptability of the tenderness is enhanced, and the problems that the tenderness is difficult to plant in different places due to strong regionality, and the method cannot adapt to common rice nutrient solution hydroponic planting are solved.

Description

Technical Field

[0001] The invention relates to the technical field of rice cultivation, and in particular to a rice nutrient solution for improving the quality of a fragrant glutinous rice variety "Gou Neng Gu". Background Art

[0002] Rice is one of the most important food crops in the world, providing food for nearly 50% of the world's population. China is a major rice producer and consumer, with two-thirds of the population relying on rice as their staple food. China has long been committed to rice breeding, focusing on improving rice yield and quality.

[0003] Xianghe glutinous rice is a special rice ecological group formed by the genetic evolution and co-evolution of Asian cultivated rice in a specific ecological region. It is one of the most mountainous and representative traditional rice resources in Guizhou. The document "Investigation on the Utilization and Protection Status of Xianghe Glutinous Rice Resources in Dong Nationality Villages in Liping County, Guizhou" (Jiao Aixia. [J]. Journal of Plant Genetic Resources, 2015, 16 (1): 173-177) mentioned that there are many types of Xianghe glutinous rice varieties, some of which still retain the characteristics of wild rice, with excellent traits such as cold resistance, barrenness resistance, drought resistance, waterlogging resistance, and shade tolerance, and the rice has excellent nutritional quality and a strong aroma. Among them, the colored rice varieties in Xianghe glutinous rice contain extremely rich nutritional elements, including protein, starch, vitamins, dietary fiber, etc., which can effectively prevent and reduce the occurrence of diseases such as atherosclerosis, cancer, and allergies. As a special germplasm resource, colored rice can not only meet people's needs for healthy diet, but also has important significance for the development of human society. These characteristics just meet the requirements of modern agricultural development, and the planting area should be increased.

[0004] However, the regionality of fragrant glutinous colored rice varieties such as "Gounen Gu" is strong, and the yield is generally low; and under conventional planting conditions, they grow poorly and have poor resistance. These problems have greatly hindered the development of large-scale, intensive and factory-based planting of "Gounen Gu". Therefore, it is necessary to study and explore a planting and cultivation method suitable for cultivating Guizhou's local high-quality specialty rice "Gounen Gu" to promote the breeding process of fragrant glutinous colored rice. Summary of the invention

[0005] Although adjusting the proportion of ammonia nitrogen and nitrate nitrogen in the nitrogen source of the rice nutrient solution, adding exogenous amino acids, adding exogenous organic acids and other methods may have a certain impact on the growth state of rice. However, for the "Gounen Gu" variety of Xianghe glutinous colored rice, there is no clear method to increase its yield in the prior art, and the only way to cultivate this rice is to explore from scratch. The present invention provides a rice nutrient solution and its application in improving the quality of the Xianghe glutinous variety "Gounen Gu", which is specifically achieved through the following technologies.

[0006] A rice nutrient solution is used for improving the quality of a fragrant glutinous rice variety "Gounen Gu". The rice nutrient solution consists of a basic nutrient solution, salicylic acid and water; or, consists of a basic nutrient solution, phenylalanine, salicylic acid and water; the concentration of the phenylalanine is 0.1-0.6 mM, and the concentration of the salicylic acid is 5-25 μM.

[0007] Furthermore, the rice nutrient solution consists of a basic nutrient solution, salicylic acid and water, and the concentration of the salicylic acid is 10-25 μM.

[0008] Furthermore, the rice nutrient solution consists of a basic nutrient solution, phenylalanine, salicylic acid and water, the concentration of the phenylalanine is 0.1-0.4 mM, and the concentration of the salicylic acid is 5-20 μM.

[0009] Furthermore, the concentration of phenylalanine is 0.4 mM, and the concentration of salicylic acid is 10 μM.

[0010] In order to screen out the best treatment conditions, the present invention adds 5 different concentrations of phenylalanine and 5 different concentrations of salicylic acid to the common rice nutrient solution (basic nutrient solution) in the experiment, forming 10 treatments; in addition, 5 different concentrations of phenylalanine are mixed with 5 different concentrations of salicylic acid and added to the common rice nutrient solution, forming 25 composite treatments. A total of 35 treatments are formed.

[0011] Finally, it was found that the rice nutrient solution with phenylalanine added alone in the basic nutrient solution had an inhibitory effect on the growth of the fragrant glutinous rice variety "Gounen Gu", which was not conducive to improving its rice quality. Adding salicylic acid alone to the basic nutrient solution, or adding 0.1-0.6mM and 5-25μM salicylic acid at the same time, can improve the growth of the fragrant glutinous rice variety "Gounen Gu" to varying degrees. Among them, adding a final concentration of 0.4mM phenylalanine and 10μM salicylic acid to the ordinary rice nutrient solution can most effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Gounen Gu", which is the optimal treatment method.

[0012] Furthermore, the formula of the basic nutrient solution is NaH2PO4·2H2O 63.000mg / L, K2SO4 111.625mg / L, CaCl2 39.55mg / L, MgSO4·7H2O 506.250mg / L, MnCl2·4H2O 1.761mg / L, Na2MoO4·2H2O 0.127mg / L, H3BO4 1.168mg / L, ZnSO4·7H2O 0.044mg / L, CuSO4·5H2O0.039mg / L, EDTA-Na2 15.000mg / L, and FeSO4·7H2O 11.140mg / L.

[0013] The present invention also provides a method for improving the quality of a fragrant glutinous rice variety "Gounen Gu", which comprises the following steps: when the "Gounen Gu" rice seeds are hydroponically cultured until the first incomplete leaf appears, the water is replaced with an ordinary rice nutrient solution, and the seeds are cultured to the three-leaf stage to obtain three-leaf rice seedlings; the rice nutrient solution is used to culture the rice seedlings in the three-leaf stage until the five-leaf stage; and the ordinary rice nutrient solution is used to continue culturing the rice seedlings until they are mature and harvested.

[0014] The present invention also provides a rice nutrient solution for improving the quality of the fragrant glutinous rice variety "Gounen Gu", which consists of a basic nutrient solution, salicylic acid and water; or, consists of a basic nutrient solution, phenylalanine, salicylic acid and water; the concentration of the phenylalanine is 0.1-0.6mM, and the concentration of the salicylic acid is 5-25μM.

[0015] Compared with the prior art, the present invention is beneficial in that:

[0016] 1. The application method of the rice nutrient solution provided by the present invention is specifically for the rice variety "Gou Neng Gu", which can solve the problem of poor growth and development of this variety in ordinary nutrient solution, especially in improving the biomass, plant height, plant nitrogen content and plant chlorophyll content of the "Gou Neng Gu" plant, and ultimately achieve a substantial increase in yield;

[0017] 2. The application method of the rice nutrient solution provided by the present invention enhances the adaptability of "Gounen Gu" to a certain extent, solves the problem that "Gounen Gu" is difficult to plant in other places due to its strong regionality, and cannot adapt to the hydroponic planting of ordinary rice nutrient solution. It is conducive to accelerating the factory-based seedling cultivation process of "Gounen Gu" and realizing the large-scale, intensive, factory-based planting management and large-scale promotion and application of "Gounen Gu". BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 The statistical results and phenotypic pictures of plant height, fresh weight, root length and dry weight of "Gou Neng Gu" plants under different concentration treatment conditions in the phenylalanine treatment group of Experimental Example 1 (statistical results are expressed as mean±SD, n=6; Duncan test was used for significant difference analysis, lowercase letters are marked above the data, in the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate the same significance level).

[0019] Figure 2Statistical results and phenotypic pictures of plant height, fresh weight, root length and dry weight of "Gou Neng Gu" plants under different concentration treatment conditions in the salicylic acid treatment group of Experimental Example 1 (statistical results are expressed as mean±SD, n=6; Duncan test was used for significant difference analysis, lowercase letters are marked above the data, in the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate consistent significance levels).

[0020] Figure 3-7 The figures are the statistical results of plant height, fresh weight, root length and dry weight of “Gou Neng Gu” plants under different concentration treatment conditions in the combined treatment group of phenylalanine and salicylic acid in Experimental Example 1, as well as phenotypic pictures (statistical results are expressed as mean±SD, n=6; Duncan test was used for significant difference analysis, with lowercase letters marked above the data; in the same statistical graph, different lowercase letters indicate significant differences, p<0.05; the same lowercase letters indicate consistent significance levels).

[0021] Figure 8 The results of the test on the nitrogen percentage content of the single plant of "Gou Neng Gu" in Example 2 of the present invention (statistical results are expressed as mean±SD, n=10; **, p<0.01; *, p<0.05).

[0022] Fig. 9 These are the test results of chlorophyll content of “Gou Neng Gu” in 3 examples of the present invention (statistical results are expressed as mean±SD, n=10; **, p<0.01; *, p<0.05). DETAILED DESCRIPTION

[0023] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] In some implementation cases of the present invention, the selected fragrant glutinous colored rice variety "Xianghe Nuo" is a high-quality specialty rice in Guizhou, which is reported in many existing documents. For example, the document "Investigation on the current status of utilization and protection of Xianghe Nuo resources in Dong ethnic village in Liping County, Guizhou" (Jiao Aixia. Journal of Plant Genetic Resources, 2015, 16 (1): 173-177), the document "Research progress on nutritional characteristics and functional components of colored rice" (Dong Jiahong, et al. Journal of Yunnan University for Nationalities (Natural Science Edition), 2023, 32 (04): 449-458), the document "Overview of research on protection and utilization of Guizhou special rice variety Xianghe Nuo germplasm resources" (Chen Huicha, et al. Seed, 2023, 42 (11): 61-67 + 2) and the document "Research on Zn · Cr content and physiological function in original ecological Danzhai selenium rice" (Tang Wenhua, et al. Anhui Agricultural Science, 2009, 37 (32): 15686-15687.).

[0025] Experimental Example 1: Effects of different phenylalanine concentrations on the growth and development of "Gou Nen Gu"

[0026] 1. Rice varieties

[0027] The "Gou Neng Gu" used in this test example is a black rice variety in "Xiang He Nuo". The biological material rice seeds used in subsequent experiments are seeds of the "Gou Neng Gu" black rice variety in "Xiang He Nuo", a local high-quality specialty rice in Guizhou.

[0028] The growth period of "Gou Nen Gu" is 170 days, the seeds are short and round, the seed shell is slightly brown, the awn rate is low, and the awns are short. The seeds of "Gou Nen Gu" were collected in Qiandongnan Prefecture, Guizhou Province, and are now stored in the Plant Hormone and Nutrition Molecular Regulation Laboratory of the Rice Industry Technology Research Institute of Guizhou University. The applicant promises to release the above two rice genetic resources to the public within 20 years from the date of application.

[0029] 2. Rice cultivation method

[0030] (1) Rice seed soaking: Soak rice seeds in 0.6% dilute nitric acid for 6-8 hours.

[0031] (2) Cultivation of rice seeds: After soaking, rinse the seeds with deionized water for 3-5 times, dispense into culture dishes, add appropriate amount of purified water, and culture in an incubator at 37°C. Change the water 1-2 times a day.

[0032] (3) Hydroponics: After the seeds germinate, wait until the buds grow to 1-2 cm, sow them in a 96-well plate and place them in a 4-L culture box with clean water until the first incomplete leaf appears; then switch to ordinary rice nutrient solution and culture until the 3-leaf stage; then transplant them into a 15-L culture pot, and add 12 L of ordinary rice nutrient solution to each pot;

[0033] Thirty-six culture pots were set up; among them, 5 culture pots were phenylalanine treatment group, 5 culture pots were salicylic acid treatment group, 25 culture pots were phenylalanine and salicylic acid combined treatment group, and 1 culture pot was a normal control group without treatment.

[0034] The formula of ordinary rice nutrient solution is a conventional nitrogen-containing culture solution currently available on the market. After extensive research, the inventors found that the conventional nitrogen-free ordinary rice nutrient solution can meet the needs of rice growth. This test example uses the formula shown in Table 1 below to prepare nitrogen-free ordinary rice nutrient solution (reference Yoshida S, Fomo DA, Cock JH, et al. Routine procedure for growing rice plants in culture solution. In: Laboratory manual for physio-logical studies of rice [J]. International Rice Research Institute, 61-66.).

[0035] Table 1 Common rice nutrient solution formula

[0036]

[0037]

[0038] The final formula of the common nutrient solution contains NaH2PO4·2H2O 63.000mg / L, K2SO4111.625mg / L, CaCl2 39.55mg / L, MgSO4·7H2O 506.250mg / L, MnCl2·4H2O 1.761mg / L, Na2MoO4·2H2O 0.127mg / L, H3BO4 1.168mg / L, ZnSO4·7H2O 0.044mg / L, CuSO4·5H2O0.039mg / L, EDTA-Na2 15.000mg / L, and FeSO4·7H2O 11.140mg / L.

[0039] Prepare 1M phenylalanine stock solution and salicylic acid stock solution for the preparation of nutrient solution. The specific preparation of the four groups in hydroponics (normal nutrient solution control group, phenylalanine treatment group, salicylic acid treatment group, phenylalanine and salicylic acid combined treatment group) is as follows:

[0040] Normal nitrogen treatment group: just add 12L of ordinary nutrient solution.

[0041] Phenylalanine treatment group: In the 12 L ordinary culture medium of the 5 culture basins of the phenylalanine treatment group, corresponding amounts (1.2 ml, 2.4 ml, 4.8 ml, 7.2 ml and 12 ml) of phenylalanine stock solution were added to the concentrations in Table 2, so that the concentrations of phenylalanine in the 5 culture basins were 0.1 mM, 0.2 mM, 0.4 mM, 0.6 mM and 1 mM, respectively.

[0042] Salicylic acid treatment group: In the 12 L common culture medium of the 5 culture pots of the salicylic acid treatment group, corresponding amounts (60 μl, 120 μl, 180 μl, 240 μl and 300 μl) of salicylic acid stock solution were added to the concentrations in Table 2, so that the concentrations of salicylic acid in the 5 culture pots were 5 μM, 10 μM, 15 μM, 20 μM and 25 μM, respectively.

[0043] Phenylalanine and salicylic acid combined treatment group: In the 12L common culture medium of 25 culture pots in the combined treatment group, the corresponding amount of mother solution was added to prepare the concentrations in Table 2. Specifically, 1.2ml of phenylalanine mother solution and 60μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 120μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 180μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 240μL of salicylic acid mother solution, 1.2ml of phenylalanine mother solution and 300μL of salicylic acid mother solution, 2.4ml of phenylalanine mother solution and 60μL of salicylic acid mother solution were added. , 2.4 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 2.4 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 2.4 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 2.4 μM phenylalanine stock solution and 300 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 60 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 4.8 ml of Phenylalanine stock solution and 180 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 240 μL of salicylic acid stock solution, 4.8 ml of phenylalanine stock solution and 300 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 60 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 120 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 180 μL of salicylic acid stock solution, 7.2 ml of phenylalanine stock solution and 240μL of salicylic acid stock solution, 7.2ml of phenylalanine stock solution and 300μL of salicylic acid stock solution, 12ml of phenylalanine stock solution and 60μL of salicylic acid stock solution, 12ml of phenylalanine stock solution and 120μL of salicylic acid stock solution, 12ml of phenylalanine stock solution and 180μL of salicylic acid stock solution, 12ml of phenylalanine stock solution and 240μL of salicylic acid stock solution, 12ml of phenylalanine stock solution and 300μL of salicylic acid stock solution.

[0044] The final concentrations in the 25 culture pots were 0.1 mM phenylalanine and 5 μM salicylic acid, 0.1 mM phenylalanine and 10 μM salicylic acid, 0.1 mM phenylalanine and 15 μM salicylic acid, 0.1 mM phenylalanine and 20 μM salicylic acid, 0.1 mM phenylalanine and 25 μM salicylic acid, 0.2 mM phenylalanine and 5 μM salicylic acid, 0.2 mM phenylalanine and 10 μM salicylic acid, 0.2 mM phenylalanine and 15 μM salicylic acid, 0.2 mM phenylalanine and 20 μM salicylic acid, 0.2 mM phenylalanine and 25 μM salicylic acid, 0.4 mM phenylalanine and 5 μM salicylic acid, and 0.4 mM phenylalanine and 10 μM salicylic acid. Salicylic acid, 0.4 mM phenylalanine and 15 μM salicylic acid, 0.4 mM phenylalanine and 20 μM salicylic acid, 0.4 mM phenylalanine and 25 μM salicylic acid, 0.5 mM phenylalanine and 5 μM salicylic acid, 0.5 mM phenylalanine and 10 μM salicylic acid, 0.5 mM phenylalanine and 15 μM salicylic acid, 0.5 mM phenylalanine and 20 μM salicylic acid, 0.5 mM phenylalanine and 25 μM salicylic acid, 1 mM phenylalanine and 5 μM salicylic acid, 1 mM phenylalanine and 10 μM salicylic acid, 1 mM phenylalanine and 15 μM salicylic acid, 1 mM phenylalanine and 20 μM salicylic acid, 1 mM phenylalanine and 25 μM salicylic acid.

[0045] Table 2: Preliminary screening treatment (Phe in the table is phenylalanine, Salicylic acid is salicylic acid, unit is mM)

[0046]

[0047]

[0048] After the rice seedlings of each treatment group have grown to the 5-leaf stage, the plant height, fresh weight, root length and dry weight of the rice seedlings of various treatment groups were counted. One seedling with a plant height close to the average plant height was selected from each treatment of the normal nitrogen control group, the organic nitrogen treatment group and the inorganic nitrogen treatment group, and the seedlings were evenly spread out on the black cloth to form a row.

[0049] The statistical results of plant phenotype, fresh weight, dry weight, plant height and root length of "Gou Neng Gu" under the treatment conditions of phenylalanine are as follows Figure 1 The statistical results of plant phenotype, fresh weight, dry weight, plant height and root length of "Gou Neng Gu" under the treatment conditions of salicylic acid are shown in Figure 1A, 1B, 1C, 1D and 1E. Figure 2 The statistical results of plant phenotype, fresh weight, dry weight, plant height and root length of "Gou Neng Gu" under the combined treatment of phenylalanine and salicylic acid are shown in Figure 2A, 2B, 2C, 2D and 2E. Figure 3-Figure 7 As shown in A, B, C, D, and E.

[0050] according to Figure 1 The experimental results show that: in the phenylalanine treatment group, the most ideal effect on promoting the growth and development of the plant height of "Gounen Gu" is to add 0.6mM phenylalanine to the ordinary rice nutrient solution, but its plant height, dry weight and root length are significantly reduced compared with the control; when phenylalanine is added, the growth and development of "Gounen Gu" plants show different degrees of inhibitory effects; especially when the phenylalanine concentration reaches 1mM, the growth and development of "Gounen Gu" shows a very obvious inhibitory effect, and its fresh weight, plant height and root length show a downward trend compared with the control, and reach a significant level, such as Figure 1 B, 1D, 1E.

[0051] according to Figure 2 The experimental results show that among the salicylic acid treatment groups, the treatment with 25 μM salicylic acid added to the ordinary nutrient solution had the most significant effect on promoting the growth and development of "Gou Nen Gu". By weighing them, it was found that in the salicylic acid treatment group, the fresh weight of "Gou Nen Gu" showed an increasing trend with the increase of salicylic acid concentration, and its dry weight was also significantly promoted under this treatment, such as Figure 2 B, 2C; while the root length of "Gou Nen Gu" showed a downward trend with the increase of salicylic acid concentration, such as Figure 2 E; When salicylic acid was added at concentrations of 5 μM and 15 μM, the plant height decreased compared to the control, and the difference was significant. Figure 2 D.

[0052] according to Figure 3-Figure 7 The experimental results show that in the phenylalanine and salicylic acid combined treatment group, the plant height and fresh weight of "Gou Nen Gu" can be significantly promoted at the same time, and the most obvious promotion effect is the treatment of adding 0.1mM phenylalanine and 5μM salicylic acid to the ordinary nutrient solution, the treatment of adding 0.2mM phenylalanine and 20μM salicylic acid to the ordinary nutrient solution, and the treatment of adding 0.4Mm phenylalanine and 10μM salicylic acid to the ordinary nutrient solution. Under these three treatments, the dry weight of "Gou Nen Gu" is also significantly higher than that of the control treatment. Figure 4 B, 4D, 5B, and 5D; when the concentration of phenylalanine exceeds 0.5mM, it is combined with salicylic acid to achieve a significant inhibitory effect on the dry weight and root length of Goutang; through the test of physiological indicators, it was found that adding 1mM phenylalanine and more than 15μM salicylic acid to ordinary rice culture medium had the most serious inhibitory effect on "Goutang".

[0053] The above results show that: (1) In the phenylalanine treatment group, the addition of phenylalanine alone on the basis of ordinary rice nutrient solution will inhibit the growth and development of "Gou Nen Gu", resulting in its poor growth and development; (2) In the salicylic acid treatment group, the treatment of adding 25 μM salicylic acid to the ordinary nutrient solution is more conducive to the growth and development of "Gou Nen Gu"; (3) In the composite treatment, adding ordinary rice nutrient solution with a final concentration of 0.4 mM phenylalanine and 10 μM salicylic acid to the ordinary rice nutrient solution can most effectively improve the plant height, biomass (fresh weight), nitrogen content, chlorophyll content and dry weight per plant of "Gou Nen Gu", which is the optimal treatment method.

[0054] Experimental Example 2: Effects of different treatments on the percentage of total nitrogen content in rice of "Gou Neng Gu"

[0055] Nitrogen is the mineral element that plants require the most, and is also known as the "element of life". When nitrogen is sufficient, plants can synthesize more protein, promote cell division and growth, plant leaf area grows faster, and photosynthesis is stronger. When nitrogen is severely deficient, organic matter synthesis is blocked, plants are short, leaves turn yellow, and old leaves turn even yellower. Therefore, the nitrogen content in plants can be used as a physiological indicator to judge the growth and development of plants.

[0056] 1. Rice plant materials

[0057] The rice materials of this example refer to Experimental Example 1. From the above-mentioned phenylalanine treatment group, salicylic acid treatment group, and phenylalanine and salicylic acid combined treatment group, the treatment groups with more obvious promotion and inhibition of the growth and development of "Gou Nen Gu" were selected, and a total of 7 treatments were made including the control group; three rice plants were taken from each treatment group, placed in an oven at 80°C and dried overnight; the dried plants were taken out and divided into leaves and stems, and the samples were crushed into powder with a grinder for later use.

[0058] 2. Determination of nitrogen content in plants

[0059] The method for measuring nitrogen content in rice plants refers to the reference book "Soil Agrochemical Analysis" (Edited by Nanjing Agricultural College. Beijing: Agricultural Press, 1980.193-197). The specific steps are:

[0060] (1) Digestion:

[0061] ① Use a 1 / 10,000 balance to weigh 0.5 g of sample (dry sample) and place it in the digestive tube.

[0062] ② Use a 1:1 balance to weigh 0.5 g of copper sulfate pentahydrate and 4.5 g of potassium sulfate and place them in the digestive tube.

[0063] ③ Use a pipette to accurately draw 8 ml of concentrated sulfuric acid and place it in the digestive tube.

[0064] ④ Turn on the instrument and start digestion. First, digest at 280℃ for about 20 minutes, then change to 350℃ for about 20 minutes, and finally change to 450℃ for digestion. Wait until the liquid in the digestion tube turns into emerald green and digest for another 30-40 minutes. Digestion is complete.

[0065] ⑤ Cool at room temperature for later use.

[0066] (2) Distillation and titration

[0067] The sample solution digested above needs to be diluted to 100ml with distilled water, 5ml of the test solution is drawn into the long glass tube of the nitrogen analyzer, and 5ml of the boric acid indicator solution is drawn into a wide-mouthed triangular flask. After distillation for 3 minutes, remove it. Titrate with a semi-micro burette, and the titration changes from green to white to pink. The obtained data is used to calculate the nitrogen content in the plant according to the following formula.

[0068] Total nitrogen in plants (%) = equivalent concentration of H2SO4 standard solution × (volume of H2SO4 standard solution consumed when titrating the sample - volume of H2SO4 standard solution in blank test) × fractionation times × 0.014 × 100 / sample weight;

[0069] In the formula: the multiple of sampling according to the above method is 20, because 5 ml is taken out of 100 mL, and the sample weight is 0.5 g. Finally, the average nitrogen content of the plants measured by the three repeated experiments in each treatment group is calculated.

[0070] The nitrogen content data of each treatment sample was statistically plotted, and the results are as follows: Figure 8 As shown, it can be seen that the difference in nitrogen content between the exogenous application of 0.4 mM phenylalanine and 10 μM salicylic acid and the control on the basis of the ordinary nutrient solution is the smallest and not significant.

[0071] Experimental Example 3: Effects of exogenous application of phenylalanine and salicylic acid on the chlorophyll content in the leaves of "Gou Neng Gu".

[0072] The chlorophyll content of rice leaves is related to the photosynthetic rate and crop growth rate. In scientific research, chlorophyll content is often measured to characterize the growth status of rice. In production, leaf color changes are often used as an important indicator for seedling diagnosis and fertilizer and water measures.

[0073] 1. The material of rice leaves

[0074] The materials of rice in this test example refer to Test Example 1. Select three rice seedlings from the above treatment group (T1 is the control), take the sword leaf part, cut the leaves except the veins into pieces, and mix them evenly. Weigh 0.2-0.3g of leaf fragments, put them in a 15ml centrifuge tube, mark the leaf weight and add 10ml of anhydrous ethanol, repeat the experiment for 3 groups, and take the average value. Wrap all centrifuge tubes containing rice leaf fragments and anhydrous ethanol with tin foil, store them away from light for 3 days, and shake them several times during the period.

[0075] 2. Determination of chlorophyll content

[0076] The method for measuring the chlorophyll content in rice leaves was carried out according to the literature "Comparison of methods for determining chlorophyll content in wheat" (Nurkemaier Murati, Yang Yajie, Parhat Abdukerimu, et al. Jiangsu Agricultural Science, 2021, 49(09): 156-159).

[0077] After 3 days, the chlorophyll extracts of all samples were obtained. Prepare 8 cuvettes, add 4 ml of anhydrous ethanol to one of them as a control, and add 3 sets of repeated chlorophyll extracts of each treated sample to the remaining 7 cuvettes. Measure the absorbance values ​​at 663nm and 645nm respectively. Calculate the chlorophyll content according to the following formula using the obtained data.

[0078] Total chlorophyll = (20.21D 645 nm +8.02D 663 nm )×V / 1000×m;

[0079] Where: D 645 nm and D 663 nm are the absorbance at 663 and 645 nm respectively; V is the volume of the test solution (ml); m is the mass of the fresh leaf sample (g) or the leaf area (cm 2 ). Finally, the total amount of chlorophyll measured by the three repeated experiments for each treatment was averaged.

[0080] The measured chlorophyll content was statistically plotted, and the results were as follows: Fig. 9 As shown. On the basis of ordinary rice nutrient solution, phenylalanine and salicylic acid were added to make the final concentrations reach 0.4mM and 10μM respectively. The total chlorophyll content of "Gou Neng Gu" was significantly increased compared with the control, and was higher than any other control.

[0081] The chlorophyll content in the leaves is an important indicator of the nutritional status of plants. A high total chlorophyll content represents a stronger photosynthesis potential and can synthesize more nutrients required for plant growth and development. This once again confirms that changing the nutrient solution formula can greatly improve the growth and development of "Gou Neng Gu" in "Xiang He Nuo", solve the problem of poor growth and development in ordinary nutrient solutions that makes it difficult to raise seedlings in factories, and accelerate the large-scale and intensive planting and management of high-quality varieties against the background of the gradual loss of rural labor.

[0082] The above specific embodiments describe the implementation of the present invention in detail, but the present invention is not limited to the specific details in the above embodiments. Within the scope of the claims and technical concept of the present invention, the technical solution of the present invention can be modified and changed in many simple ways, and these simple modifications all belong to the protection scope of the present invention.

Claims

1. An application of a rice nutrient solution in improving the quality of a fragrant glutinous rice variety "Gou Neng Gu", characterized in that: The rice nutrient solution consists of a basic nutrient solution, salicylic acid and water; or, consists of a basic nutrient solution, phenylalanine, salicylic acid and water; the concentration of the phenylalanine is 0.1-0.6 mM, and the concentration of the salicylic acid is 5-25 μM.

2. The use of the rice nutrient solution according to claim 1 in improving the quality of the fragrant glutinous rice variety "Gou Neng Gu", characterized in that: The rice nutrient solution consists of a basic nutrient solution, salicylic acid and water, and the concentration of the salicylic acid is 10-25 μM.

3. The use of the rice nutrient solution according to claim 1 in improving the quality of the fragrant glutinous rice variety "Gou Neng Gu", characterized in that: The rice nutrient solution consists of a basic nutrient solution, phenylalanine, salicylic acid and water. The concentration of the phenylalanine is 0.1-0.4 mM, and the concentration of the salicylic acid is 5-20 μM.

4. The use of the rice nutrient solution according to claim 3 in improving the quality of the Xianghe glutinous rice variety "Gou Neng Gu", wherein the concentration of phenylalanine in the rice nutrient solution is 0.4 mM and the concentration of salicylic acid is 10 μM.

5. The use of the rice nutrient solution according to claim 1 in improving the quality of the fragrant glutinous rice variety "Gou Neng Gu", characterized in that: The formula of the basic nutrient solution is NaH2PO4·2H2O 63.000mg / L, K2SO4 111.625mg / L, CaCl2 39.55mg / L, MgSO4·7H2O 506.250mg / L, MnCl2·4H2O 1.761mg / L, Na2MoO4·2H2O 0.127mg / L, H3BO4 1.168mg / L, ZnSO4·7H2O 0.044mg / L, CuSO4·5H2O 0.039mg / L, EDTA-Na2 15.000mg / L, and FeSO4·7H2O 11.140mg / L.

6. A method for improving the quality of the fragrant glutinous rice variety "Gou Neng Gu", characterized in that: When the "Gou Neng Gu" rice seeds are hydroponically cultured until the first incomplete leaf appears, the water is replaced with an ordinary rice nutrient solution, and the seeds are cultured to the three-leaf stage to obtain three-leaf rice seedlings; the rice nutrient solution of any one of claims 1 to 5 is used to culture the rice seedlings in the three-leaf stage until the five-leaf stage; and the ordinary rice nutrient solution is used to continue culturing the rice seedlings until they are mature and harvested.

7. A rice nutrient solution, characterized in that: The rice nutrient solution is used to improve the quality of the fragrant glutinous rice variety "Gounen Gu", and the formula consists of a basic nutrient solution, salicylic acid and water; or, consists of a basic nutrient solution, phenylalanine, salicylic acid and water; the concentration of the phenylalanine is 0.1-0.6mM, and the concentration of the salicylic acid is 5-25μM.

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