Multifunctional microbial culture medium and application thereof in environmental protection and agriculture

Abstract

The invention discloses a multifunctional microbial culture medium and application thereof in environmental protection and agriculture, through combination of bacillus licheniformis, lactic acid bacteria and saccharomycetes, pathogenic microorganisms can be effectively inhibited, the biological activity and safety of the culture medium are improved, microbial strains have a synergistic effect in the fermentation process, decomposition of organic matters is accelerated, and the biological activity of the culture medium is improved. In addition, a plurality of net-shaped hollow pipes which are distributed alternately are arranged, phase change strips and moisture absorption strips wrap the net-shaped hollow pipes, heat and moisture are conducted through guide fibers, sufficient oxygen supply is provided through the design of ventilation holes of the net-shaped hollow pipes, and good ventilation and protection are ensured through a ventilation protection cover made of Gore-Tex. Automatic adjustment of temperature, humidity and ventilation in the fermentation process is achieved, the optimal growth condition of microorganisms is ensured, resource utilization of waste is achieved, production benefits are improved, and cyclic utilization of resources and protection of the ecological environment are promoted.

Description

Technical Field

[0001] The invention relates to the technical field of microbial strains, and more specifically to a multifunctional microbial culture medium and its application in environmental protection and agriculture. Background Art

[0002] In modern agriculture and aquaculture, the application of microbial culture medium is becoming more and more widespread. This culture medium is mainly composed of organic waste and microbial strains. Through microbial fermentation, organic matter is converted into valuable resources, such as organic fertilizers, feed additives, etc. Microbial culture medium not only plays an important role in improving agricultural output and aquaculture benefits, but also has important significance in environmental protection and resource recycling. Common microbial culture medium materials include sawdust, straw, waste bacteria bags, animal manure, etc. These materials are rich in organic matter and nutrients, providing good conditions for the growth of microorganisms.

[0003] Although microbial culture media are widely used in agriculture and aquaculture, there are still some problems in actual use. First, the fermentation process of microbial culture media is greatly affected by environmental conditions such as temperature, humidity and ventilation, and it is difficult to stably control, resulting in unstable fermentation efficiency and product quality. Secondly, although traditional culture media materials provide rich organic matter, they are limited in inhibiting harmful microorganisms and easily lead to the reproduction of pathogens, affecting the health of crops and animals. In addition, the potential of existing microbial culture media in resource utilization has not been fully explored. How to more efficiently convert agricultural waste into valuable resources is still a problem that needs to be solved. An improved microbial culture medium and its preparation method are needed, which can show excellent performance and stable effects in a variety of application scenarios.

[0004] Therefore, in view of the above technical problems, it is necessary to provide a multifunctional microbial culture medium and its application in environmental protection and agriculture. Summary of the invention

[0005] The object of the present invention is to provide a multifunctional microbial culture medium and its application in environmental protection and agriculture to solve the above-mentioned problems.

[0006] In order to achieve the above purpose, the technical solution provided by an embodiment of the present invention is as follows: A microbial culture medium comprises the following components: wood chips, straws, waste bacteria bags, animal excrement and microbial strains.

[0007] As a further improvement of the present invention, the microbial strains are any one or more of Bacillus licheniformis, lactic acid bacteria or yeast.

[0008] Any one of the following applications of the microbial culture medium includes: a: Healthy livestock and poultry breeding: used to lay microbial culture medium in breeding sites, treat manure and bedding through microbial fermentation, reduce harmful bacteria, reduce the incidence of animal diseases, and improve the health of livestock and poultry; b: Wastewater treatment and recycling: used to treat wastewater generated during aquaculture, decompose organic matter through microbial fermentation, reduce pollutant emissions, and achieve wastewater recycling; c: Bio-organic fertilizer production: Use fermented microbial culture medium to produce high-efficiency bio-fertilizer, improve soil structure, and increase soil organic matter content; d: Agricultural planting: used in agricultural planting to improve soil, promote crop growth, and increase crop yield and quality; e: Waste resource utilization: Agricultural waste such as straw and waste mushroom bags are converted into valuable resources through microbial fermentation to achieve waste resource utilization.

[0009] The method for screening microbial strains for antibiotic-free breeding comprises the following steps: S41: Collect microbial samples from natural environments: Collect microbial samples from soil, plants and animal excrement in various natural environments to obtain diverse bacterial resources; S42: Screening out strains with excellent antibacterial properties by using the method of bedding fermentation of biocontrol bacteria: inoculating the collected microbial samples into a culture medium containing sawdust, straw and waste bacterial bag bedding, and testing the antibacterial properties of the microorganisms through the fermentation process. The specific process is as follows: S421: Mix the litter with an appropriate amount of water and adjust to a suitable humidity; S422: Inoculate microbial samples and ferment under appropriate temperature and ventilation conditions; S423: Regularly test the antibacterial substances produced during the fermentation process, and screen out strains with excellent antibacterial properties through inhibition zone experiments or antibacterial activity assays; S43: From the strains screened in step S42, based on a comprehensive evaluation of fermentation performance, antibacterial effect and adaptability, Bacillus licheniformis is finally selected as the main strain.

[0010] As a further improvement of the present invention, the antibiotic-free cultured microbial strain combination includes Bacillus licheniformis, lactic acid bacteria and yeast, and the strain combination is used to enhance the fermentation effect and disease resistance.

[0011] The preparation method of the microbial culture medium comprises the following steps: S71: Mix wood chips, straw, waste bacteria bags and animal manure according to the mass percentage: Wood chips: 30-40%; Straw: 20-30%; Waste bacteria bags: 10-20%; Animal manure: 20-30%; S72: Inoculation with Bacillus licheniformis: S721: Prepare a bacterial suspension of Bacillus licheniformis with a concentration of 1× CFU / mL; S722: Add the bacterial suspension to the mixture at 0.1-1% (w / w) of the mixture weight; S723: spraying the bacterial suspension evenly onto the mixture while stirring to ensure that all the mixture is evenly inoculated; S73: Fermentation under suitable temperature and humidity conditions: S731: placing the inoculated mixture in a fermentation container and maintaining the temperature between 25-35°C; S732: Adjust the humidity of the mixture to 55-65% and maintain the humidity by spraying an appropriate amount of water regularly; S733: Good ventilation should be ensured during the fermentation process to promote the growth of microorganisms and fermentation reactions; S734: Fermentation time is 7-14 days, and the specific time is adjusted according to the fermentation process and environmental conditions; The fermentation container is provided with a plurality of alternately distributed mesh hollow tubes, and the plurality of mesh hollow tubes are surrounded by phase change strips and moisture absorption strips and arranged in a spiral structure. The outer surfaces of the phase change strips and moisture absorption strips are provided with a plurality of guide fibers for conducting heat and moisture. The outer surfaces of the mesh hollow tubes are provided with a plurality of air holes, and the air holes are covered with a breathable and waterproof protective film. The top of the fermentation container is provided with a protective cover made of breathable material.

[0012] As a further improvement of the present invention, the material of the phase change strip is fatty acid or paraffin, the material of the moisture absorption strip is silica gel or activated carbon, and the breathable material is Gore-Tex.

[0013] The antibiotic-free breeding method comprises the following steps: S81: preparing a microbial culture medium according to a method for preparing a microbial culture medium for antibiotic-free cultivation; S82: laying the microbial culture medium on the breeding site; S83: Regularly supplement Bacillus licheniformis during the breeding process.

[0014] As a further improvement of the present invention, the Bacillus licheniformis can reduce harmful bacteria in the breeding environment and reduce the incidence of animal diseases.

[0015] As a further improvement of the present invention, the microbial fertilizer is fermented from used microbial culture medium, and the main bacterial species is Bacillus licheniformis.

[0016] Compared with the prior art, the advantages of the present invention are: (1) This scheme provides an improved microbial culture medium and its preparation method. Through scientific methods and innovative designs, the fermentation efficiency and product quality are significantly improved. First, the culture medium is composed of sawdust, straw, waste bacteria bags, animal manure and microbial strains. These components are mixed in a specific proportion to provide rich organic matter and multiple nutrients to promote the growth and reproduction of microorganisms. The combination of Bacillus licheniformis, lactic acid bacteria and yeast can effectively inhibit pathogenic microorganisms and improve the biological activity and safety of the culture medium. These microbial strains work synergistically during the fermentation process to accelerate the decomposition of organic matter and improve fermentation efficiency.

[0017] (2) This scheme arranges a plurality of alternately distributed mesh hollow tubes and wraps phase change strips and moisture absorption strips on the outside thereof, using guide fibers to conduct heat and moisture. The air hole design of the mesh hollow tubes provides sufficient oxygen supply, and the breathable protective cover made of Gore-Tex ensures good ventilation and protection, thereby realizing automatic adjustment of temperature, humidity and ventilation during the fermentation process and ensuring the optimal growth conditions for microorganisms. Phase change strips made of fatty acids or paraffin and moisture absorption strips made of silica gel or activated carbon not only adjust environmental conditions but also do not release harmful substances, thereby ensuring that the fermentation process is efficient and safe. This microbial culture medium is not only suitable for antibiotic-free breeding, but can also be used for sewage treatment, agricultural planting and bio-organic fertilizer production, thereby realizing the resource utilization of waste, improving production efficiency, and promoting the recycling of resources and the protection of the ecological environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the mesh hollow tube of the present invention; Figure 2 It is a schematic diagram of the split structure of the mesh hollow tube of the present invention; Figure 3 This is a schematic diagram of the detection data structure according to an embodiment of the present invention. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work are within the scope of protection of the present invention.

[0020] Example 1: Healthy livestock and poultry farming A microbial culture medium includes the microbial culture medium, which includes the following components: wood chips, straw, waste bacteria bags, animal excrement and microbial strains.

[0021] The microbial strains are any one or more of Bacillus licheniformis, lactic acid bacteria or yeast.

[0022] Among them, the culture medium is composed of sawdust, straw, waste bacteria bags, animal manure and microbial strains. These ingredients are mixed in a specific proportion to provide rich organic matter and nutrients suitable for the growth of microorganisms. The microbial strains include one or more of Bacillus licheniformis, lactic acid bacteria or yeast. Each of the above strains has unique functions: Bacillus licheniformis can produce antibacterial substances and inhibit harmful microorganisms, lactic acid bacteria can improve digestion and enhance immunity, while yeast helps to decompose organic matter and promote the release and absorption of nutrients. Through the synergistic effect of microbial strains in the fermentation process, the decomposition of organic matter can be accelerated, the fermentation efficiency can be improved, and the overall environmental quality can be improved. It is not only suitable for antibiotic-free breeding, but also can be used for sewage treatment, agricultural planting and bio-organic fertilizer production to realize the resource utilization of waste.

[0023] Any of the following applications of a microbial culture medium includes: a: Healthy livestock and poultry breeding: used to lay microbial culture medium in breeding sites, treat manure and bedding through microbial fermentation, reduce harmful bacteria, reduce the incidence of animal diseases, and improve the health of livestock and poultry; b: Wastewater treatment and recycling: used to treat wastewater generated during aquaculture, decompose organic matter through microbial fermentation, reduce pollutant emissions, and achieve wastewater recycling; c: Bio-organic fertilizer production: Use fermented microbial culture medium to produce high-efficiency bio-fertilizer to improve soil structure and increase soil organic matter content; d: Agricultural planting: used in agricultural planting to improve soil, promote crop growth, and increase crop yield and quality; e: Waste resource utilization: Agricultural waste such as straw and waste mushroom bags are converted into valuable resources through microbial fermentation to achieve waste resource utilization.

[0024] The culture medium is composed of sawdust, straw, waste bacteria bags, animal manure and microbial strains, including one or more of Bacillus licheniformis, lactic acid bacteria or yeast. Through scientific preparation and application, it has shown broad application prospects and significant effects in many fields such as healthy livestock and poultry breeding, sewage treatment, bio-organic fertilizer production, agricultural planting and waste resource utilization, which not only improves production efficiency, but also promotes the recycling of resources and the protection of the ecological environment.

[0025] The method for screening microbial strains for antibiotic-free farming comprises the following steps: S41: Collect microbial samples from natural environments: Collect microbial samples from soil, plants and animal excrement in various natural environments to obtain diverse bacterial resources; S42: Screening out strains with excellent antibacterial properties by using the method of bedding fermentation of biocontrol bacteria: inoculating the collected microbial samples into a culture medium containing sawdust, straw and waste bacterial bag bedding, and testing the antibacterial properties of the microorganisms through the fermentation process. The specific process is as follows: S421: Mix the litter with an appropriate amount of water and adjust to a suitable humidity; S422: Inoculate microbial samples and ferment under appropriate temperature and ventilation conditions; S423: Regularly test the antibacterial substances produced during the fermentation process, and screen out strains with excellent antibacterial properties through inhibition zone experiments or antibacterial activity assays; S43: From the strains screened in step S42, based on a comprehensive evaluation of fermentation performance, antibacterial effect and adaptability, Bacillus licheniformis is finally selected as the main strain.

[0026] The combination of microbial strains for antibiotic-free farming includes Bacillus licheniformis, lactic acid bacteria and yeast, and the combination of strains is used to enhance the fermentation effect and disease resistance.

[0027] Among them, by collecting microbial samples from the natural environment, rich and diverse bacterial resources are obtained, which provides a broad basis for screening excellent bacterial strains. The method of fermenting biocontrol bacteria in litter is used to detect the antibacterial properties of microorganisms through the fermentation process, and accurately screen out bacterial strains with excellent antibacterial properties. A comprehensive evaluation is conducted based on fermentation performance, antibacterial effect and adaptability to ensure that the selected bacterial strains perform well in practical applications. The bacterial strain combination (Bacillus licheniformis, lactic acid bacteria and yeast) significantly enhances the fermentation effect and disease resistance through synergistic effects, and improves the overall performance of the culture medium. The microbial culture medium and its preparation method provide an efficient and safe solution for antibiotic-free farming, reduce the use of antibiotics, and meet the requirements of ecological protection and sustainable development.

[0028] See also Figure 1-3 , the preparation method of the microbial culture medium comprises the following steps: S71: Mix wood chips, straw, waste bacteria bags and animal manure according to the mass percentage: Wood chips: 30-40%; Straw: 20-30%; Waste bacteria bags: 10-20%; Animal manure: 20-30%; S72: Inoculation with Bacillus licheniformis: S721: Prepare a bacterial suspension of Bacillus licheniformis with a concentration of 1× CFU / mL; S722: Add the bacterial suspension to the mixture at 0.1-1% (w / w) of the mixture weight; S723: spraying the bacterial suspension evenly onto the mixture while stirring to ensure that all the mixture is evenly inoculated; S73: Fermentation under suitable temperature and humidity conditions: S731: placing the inoculated mixture in a fermentation container and maintaining the temperature between 25-35°C; S732: Adjust the humidity of the mixture to 55-65% and maintain the humidity by spraying an appropriate amount of water regularly; S733: Good ventilation should be ensured during the fermentation process to promote the growth of microorganisms and fermentation reactions; S734: Fermentation time is 7-14 days, and the specific time is adjusted according to the fermentation process and environmental conditions; A plurality of alternately distributed mesh hollow tubes are arranged in the fermentation container, and phase change strips and moisture absorption strips are installed around the plurality of mesh hollow tubes in a spiral structure. The outer surfaces of the phase change strips and moisture absorption strips are provided with a plurality of guide fibers for conducting heat and moisture. A plurality of air holes are provided on the outer surface of the mesh hollow tubes, and the air holes are covered with a breathable and waterproof protective film. A protective cover made of breathable material is provided on the top of the fermentation container.

[0029] The phase change strips are made of fatty acids or paraffin, the moisture absorbing strips are made of silica gel or activated carbon, and the breathable material is Gore-Tex.

[0030] First, wood chips, straw, waste bacteria bags and animal manure were mixed according to mass percentage (wood chips accounted for 35%, straw accounted for 25%, waste bacteria bags accounted for 15%, and animal manure accounted for 25%), and then Bacillus licheniformis suspension was prepared (the concentration of the bacterial suspension was 1× CFU / mL), add the bacterial suspension to the mixture at 0.1% of the weight of the mixture, spray and stir evenly, then place the inoculated mixture in a fermentation container, ferment at a temperature of 25-35°C and a humidity of 55-65%, ensure good ventilation, and adjust the fermentation time to 10 days. The test data show: bacteria reduction rate: 85%; animal disease incidence rate reduced: 75%; livestock and poultry health level improved: significantly.

[0031] The fermentation container is equipped with multiple alternating mesh hollow tubes, which are wrapped with phase change strips made of fatty acids or paraffin and moisture absorption strips made of silica gel or activated carbon and arranged in a spiral structure. The outer surfaces of the phase change strips and moisture absorption strips are provided with guide fibers. The outer surface of the mesh hollow tubes is provided with air holes and covered with a breathable and waterproof protective film. The top of the container is provided with a breathable protective cover made of Gore-Tex. This design achieves automatic adjustment of temperature, humidity and ventilation during the fermentation process through optimization of materials and structures, thereby ensuring the optimal growth conditions of microorganisms.

[0032] Fatty acids are naturally present in animal and plant oils and fats, and are environmentally friendly and non-toxic. Commonly used ones include stearic acid and palmitic acid. Paraffin is a petroleum extract that is commonly used in insulation materials and food packaging. It has stable chemical properties and will not react with microorganisms. The above-mentioned phase change materials usually do not release harmful substances at conventional concentrations and will not inhibit microorganisms.

[0033] Silica gel is a highly porous adsorption material, commonly used for drying food, medicine and electronic products, with stable chemical properties, non-toxic and harmless. Activated carbon has excellent adsorption capacity and is widely used in water treatment and air purification. It has stable chemical properties and is non-toxic to microorganisms. The above-mentioned hygroscopic materials do not release harmful chemicals and do not affect the growth and fermentation process of microorganisms.

[0034] Gore-Tex is made of polytetrafluoroethylene (PTFE), which is breathable and waterproof and is widely used in outdoor clothing, medical equipment, etc. The chemical properties are extremely stable and non-toxic. Gore-Tex materials will not release harmful substances, and due to its breathability, it can effectively prevent the accumulation of harmful gases during the fermentation process, thereby not affecting the growth of microorganisms.

[0035] The guiding fiber uses food grade or medical grade non-toxic fibers, such as polyester fibers. Polyester fibers are often used in food packaging and medical devices. They have good heat resistance and chemical stability, are non-toxic and harmless, and the fiber materials will not release harmful substances and will not have a negative impact on microorganisms in the fermentation process.

[0036] Phase change strips made of fatty acids or paraffin and moisture-absorbing strips made of silica gel or activated carbon are used to achieve adaptive regulation of temperature and humidity, avoiding overheating, overhumidity or overdryness during the fermentation process. The guiding fibers ensure that heat and moisture are evenly distributed in the fermentation container to prevent local temperature and humidity unevenness. The mesh hollow tube and air hole design provide sufficient oxygen supply to promote the healthy growth of microorganisms. The breathable protective cover made of Gore-Tex can prevent external pollution while maintaining good ventilation to ensure a stable fermentation environment.

[0037] All materials are stable and non-toxic options that will not have a negative impact on microorganisms, ensuring that the fermentation process is efficient and safe. This microbial culture medium is not only suitable for antibiotic-free farming, but can also be used for sewage treatment, agricultural planting, and bio-organic fertilizer production to achieve resource utilization of waste.

[0038] Compared with the existing technology, the pathogen reduction rate has been significantly improved, indicating that the microbial culture medium is effective in inhibiting pathogenic microorganisms. The incidence of animal diseases has been reduced by 75%, which means that the culture medium has significantly improved the breeding environment, reduced the spread of pathogens, and significantly improved the health level of livestock and poultry. This reflects the huge potential of the culture medium in practical applications and can improve the production performance and growth quality of livestock and poultry.

[0039] The antibiotic-free farming method includes the following steps: S81: preparing a microbial culture medium according to a method for preparing a microbial culture medium for antibiotic-free cultivation; S82: laying the microbial culture medium on the breeding site; S83: Regularly supplement Bacillus licheniformis during the breeding process.

[0040] Bacillus licheniformis can reduce harmful pathogens in the breeding environment and reduce the incidence of animal diseases.

[0041] Microbial fertilizer is fermented from used microbial culture medium, and the main bacteria species is Bacillus licheniformis.

[0042] Example 2: Wastewater treatment and recycling Preparation of microbial culture medium: Mix wood chips, straw, waste bacteria bags and animal manure according to mass percentage (35% wood chips, 25% straw, 15% waste bacteria bags, and 25% animal manure).

[0043] Prepare the suspension of Bacillus licheniformis (the concentration of the suspension is 1× CFU / mL), add the bacterial suspension to the mixture at 0.5% of the mixture weight, and spray and stir evenly.

[0044] Fermentation process: The mixture is placed in a fermentation container, maintained at a temperature between 25-35°C and a humidity between 55-65%. Ensure good ventilation and fermentation time is 12 days.

[0045] Phase change strips (stearic acid) and hygroscopic strips (silica gel) are used for temperature and humidity regulation, and the mesh hollow tube and air permeable hole design ensures oxygen supply.

[0046] Wastewater treatment: The fermented culture medium is used to treat the wastewater generated during the breeding process, and the organic matter is decomposed through microbial fermentation.

[0047] Test data: Organic matter decomposition rate: 90%; Pollutant emissions reduction: 80%; Wastewater recycling rate: 75%.

[0048] The decomposition rate of organic matter reaches 90%. The microbial culture medium has extremely high efficiency in decomposing organic matter and can significantly reduce organic pollutants in sewage; pollutant emissions are reduced by 80%, showing the superior performance of the culture medium in environmental protection and helping to reduce the pollution of the aquaculture industry to the surrounding environment; the 75% sewage recycling rate shows that the culture medium can effectively treat aquaculture sewage, realize the recycling of sewage, and greatly reduce the waste of water resources.

[0049] Example 3: Bio-organic fertilizer production Preparation of microbial culture medium: Mix wood chips, straw, waste bacteria bags and animal manure according to mass percentage (35% wood chips, 25% straw, 15% waste bacteria bags, and 25% animal manure).

[0050] Prepare the suspension of Bacillus licheniformis (the concentration of the suspension is 1× CFU / mL), add the bacterial suspension to the mixture at 1% of the mixture weight, and spray and stir evenly.

[0051] Fermentation process: The mixture is placed in a fermentation container, maintained at a temperature between 25-35°C and a humidity between 55-65%, ensuring good ventilation, and the fermentation time is 14 days.

[0052] Phase change strips (stearic acid) and hygroscopic strips (silica gel) are used for temperature and humidity regulation, and the mesh hollow tube and air permeable hole design ensures oxygen supply.

[0053] Fertilizer production: Use fermented microbial culture medium to produce high-efficiency biological fertilizer.

[0054] Test data: Soil structure improvement: Significant; Increase in soil organic matter content: 55%; Crop growth promotion: significant.

[0055] The culture medium significantly improved the soil structure, increased the soil's air permeability and water retention, and provided a better environment for crop growth; the soil organic matter content increased by 55%, which shows that the culture medium is effective in improving soil fertility and helps to increase crop yield and quality; crop growth is significantly promoted, reflecting the application value of the culture medium in agricultural planting and can greatly improve the economic benefits of crops.

[0056] In general, the test results of the above examples show the superior performance of the microbial culture medium in a variety of application scenarios: Healthy livestock and poultry breeding: significantly reduce pathogens, reduce the incidence of animal diseases, and improve the health level of livestock and poultry, which has important practical application value; Wastewater treatment and recycling: Efficiently decompose organic matter, significantly reduce pollutant emissions, realize wastewater recycling, and have good environmental protection effects; Bio-organic fertilizer production: improve soil structure, increase soil organic matter content, promote crop growth, and enhance agricultural production efficiency; Through these applications, the microbial culture medium not only improves production efficiency, but also promotes the recycling of resources and the protection of the ecological environment, showing broad application prospects and significant social benefits.

[0057] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0058] In addition, it should be understood that although the present specification is described according to embodiments, not every embodiment contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation methods that those skilled in the art can understand.

Claims

1. A microbial culture medium, characterized in that: The microbial culture medium comprises the following components: wood chips, straws, waste bacteria bags, animal excrement and microbial strains.

2. A microbial culture medium according to claim 1, characterized in that: The microbial strains are any one or more of Bacillus licheniformis, lactic acid bacteria or yeast.

3. Any one of the following uses of a microbial culture medium according to claim 1, characterized in that: include: a: Healthy livestock and poultry breeding: used to lay microbial culture medium in breeding sites, treat manure and bedding through microbial fermentation, reduce harmful bacteria, reduce the incidence of animal diseases, and improve the health of livestock and poultry; b: Wastewater treatment and recycling: used to treat wastewater generated during aquaculture, decompose organic matter through microbial fermentation, reduce pollutant emissions, and achieve wastewater recycling; c: Bio-organic fertilizer production: Use fermented microbial culture medium to produce high-efficiency bio-fertilizer, improve soil structure, and increase soil organic matter content; d: Agricultural planting: used in agricultural planting to improve soil, promote crop growth, and increase crop yield and quality; e: Waste resource utilization: Agricultural waste such as straw and waste mushroom bags are converted into valuable resources through microbial fermentation to achieve waste resource utilization.

4. A method for screening microbial strains for antibiotic-free breeding, characterized in that: The following steps are involved: S41: Collect microbial samples from natural environments: Collect microbial samples from soil, plants and animal excrement in various natural environments to obtain diverse bacterial resources; S42: Screening out strains with excellent antibacterial properties by using the method of bedding fermentation of biocontrol bacteria: inoculating the collected microbial samples into a culture medium containing sawdust, straw and waste bacterial bag bedding, and testing the antibacterial properties of the microorganisms through the fermentation process. The specific process is as follows: S421: Mix the litter with an appropriate amount of water and adjust to a suitable humidity; S422: Inoculate microbial samples and ferment under appropriate temperature and ventilation conditions; S423: Regularly test the antibacterial substances produced during the fermentation process, and screen out strains with excellent antibacterial properties through inhibition zone experiments or antibacterial activity assays; S43: From the strains screened in step S42, based on a comprehensive evaluation of fermentation performance, antibacterial effect and adaptability, Bacillus licheniformis is finally selected as the main strain.

5. A microbial strain combination for antibiotic-free farming, characterized in that: The invention comprises bacillus licheniformis, lactic acid bacteria and yeast, and the bacterial species combination is used for enhancing the fermentation effect and disease resistance.

6. A method for preparing a microbial culture medium for antibiotic-free breeding, characterized in that: The following steps are involved: S71: Mix wood chips, straw, waste bacteria bags and animal manure according to the mass percentage: Wood chips: 30-40%; Straw: 20-30%; Waste bacteria bags: 10-20%; Animal waste: 20-30%; S72: Inoculation with Bacillus licheniformis: S721: Prepare a bacterial suspension of Bacillus licheniformis with a concentration of 1× CFU / mL; S722: Add the bacterial suspension to the mixture at 0.1-1% (w / w) of the mixture weight; S723: spraying the bacterial suspension evenly onto the mixture while stirring to ensure that all the mixture is evenly inoculated; S73: Fermentation under suitable temperature and humidity conditions: S731: placing the inoculated mixture in a fermentation container and maintaining the temperature between 25-35°C; S732: Adjust the humidity of the mixture to 55-65% and maintain the humidity by spraying an appropriate amount of water regularly; S733: Good ventilation should be ensured during the fermentation process to promote the growth of microorganisms and fermentation reactions; S734: Fermentation time is 7-14 days, and the specific time is adjusted according to the fermentation process and environmental conditions; The fermentation container is provided with a plurality of alternately distributed mesh hollow tubes, and the plurality of mesh hollow tubes are surrounded by phase change strips and moisture absorption strips and arranged in a spiral structure. The outer surfaces of the phase change strips and moisture absorption strips are provided with a plurality of guide fibers for conducting heat and moisture. The outer surfaces of the mesh hollow tubes are provided with a plurality of air holes, and the air holes are covered with a breathable and waterproof protective film. The top of the fermentation container is provided with a protective cover made of breathable material.

7. The method for preparing a microbial culture medium for antibiotic-free breeding according to claim 6, characterized in that: The material of the phase change strip is fatty acid or paraffin, the material of the moisture absorption strip is silica gel or activated carbon, and the breathable material is Gore-Tex.

8. An antibiotic-free breeding method based on microbial strains, characterized in that: The method for preparing a microbial culture medium for antibiotic-free aquaculture according to claim 7, wherein the antibiotic-free aquaculture method comprises the following steps: S81: preparing a microbial culture medium according to a method for preparing a microbial culture medium for antibiotic-free cultivation; S82: laying the microbial culture medium on the breeding site; S83: Regularly supplement Bacillus licheniformis during the breeding process.

9. The antibiotic-free breeding method according to claim 8, characterized in that: The Bacillus licheniformis can reduce harmful bacteria in the breeding environment and reduce the incidence of animal diseases.

10. A microbial fertilizer for antibiotic-free farming, characterized in that: The microbial fertilizer is fermented from used microbial culture medium, and the main bacterial species is Bacillus licheniformis.

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