Flow cytometer capable of improving particle resolution and use method thereof

Abstract

The invention belongs to the field of cell detection, and particularly relates to a flow cytometer capable of improving particle resolution and a use method thereof.The flow cytometer comprises a protective shell, a first lens, a second lens and an optical filter, a partition plate is welded and fixed in the protective shell, an adjustable flow chamber is installed in the protective shell, and the first lens and the second lens are arranged in the adjustable flow chamber; a uniform processing assembly is installed in the protective shell, a second hydraulic rod is installed at the top end of the interior of the protective shell, a mounting plate is fixedly connected to the bottom end of the second hydraulic rod, a first through hole is formed in the mounting plate in a penetrating mode, and positioning blocks are fixedly connected to the top ends of a first lens, a second lens and an optical filter. A laser is fixedly installed on the side end face of the partition plate, a self-cleaning assembly is installed in the protective shell, and the problem that an existing flow cytometer cannot conveniently adjust the diameter of a flowing channel according to different types of samples is solved; and each optical filter needing to be used cannot be automatically cleaned.

Description

Technical Field

[0001] The invention relates to the field of cell detection, in particular to a flow cytometer capable of improving particle resolution and a use method thereof. Background Art

[0002] Flow cytometry is a technology used for cell analysis and is widely used in biomedical research, clinical diagnosis, biopharmaceuticals and other fields. Its working principle is to suspend single cells in liquid, irradiate them with lasers or other light sources, and use photodetectors to analyze the characteristics of the cells. Flow cytometer is a powerful tool that can help researchers and clinicians deeply understand the characteristics and functions of cells, and is of great significance in basic research and clinical applications.

[0003] However, existing flow cytometers have some problems in their actual working process. For example, a flow cytometer with publication number CN116698710A can adjust the internal temperature and humidity environment of the device, but in its actual working process, it cannot conveniently adjust the diameter of the flow channel according to different types of samples (such as cell suspensions, particles, etc.), which will affect the flow characteristics of the sample, resulting in low particle resolution and inaccurate detection results; and in its actual working process, it cannot automatically clean each optical filter that needs to be used, and cannot conveniently replace the appropriate optical filter, which leads to poor signal capture, thereby affecting the accuracy of the data. Therefore, it is necessary to provide a flow cytometer that can improve particle resolution and a method of using it to meet the needs of users. Summary of the invention

[0004] In view of the problems existing in the existing flow cytometers capable of improving particle resolution and the use methods thereof, the present invention is proposed.

[0005] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions: a flow cytometer capable of improving particle resolution, comprising a protective shell, a first lens, a second lens and a filter, a partition plate is welded and fixed inside the protective shell, a first door panel and a second door panel are installed on the protective shell, a first hydraulic rod is installed at the bottom end of the protective shell, a placement frame is fixedly connected to the top end of the first hydraulic rod, a test tube is arranged in the placement frame, an adjustable flow chamber is installed in the protective shell, a uniform processing component is installed in the protective shell, a support plate is welded and fixed on the inner wall of the protective shell and the side end surface of the partition plate, a second hydraulic rod is installed at the top end of the protective shell, and the The bottom end of the second hydraulic rod is fixedly connected to a mounting plate, a first through hole is penetrated through the mounting plate, the top ends of the first lens, the second lens and the filter are fixedly connected to positioning blocks, a laser is fixedly installed on the side end face of the partition plate, a first detector and a second detector are fixedly installed on the inner wall of the protective shell, a self-cleaning component is installed in the protective shell, a collecting box is slidably connected to the bottom side end of the protective shell, a magnetic plate is fixedly connected to the side end face of the collecting box, the magnetic plate is magnetically adsorbed and connected to the bottom side end face of the protective shell, a second sponge frame is arranged in the collecting box, a sponge plate is fixedly connected in the second sponge frame, and a control panel is fixedly installed on the side end face of the protective shell.

[0006] As a preferred solution of the present invention, the outer wall of the test tube is in contact with the inner wall of the placement frame, the top surface of the support plate is in contact with the bottom surface of the mounting plate, four second hydraulic rods are arranged, and the four second hydraulic rods are evenly distributed around the mounting plate, the second lenses are symmetrically distributed on the front and back sides of the protective shell, the filters are equidistantly distributed on the front and back sides of the protective shell, the filters are inclined, the filters correspond one-to-one with the second detectors, the collection box is in contact with the inner wall of the protective shell, the inner wall of the collection box is in contact with the outer wall of the second sponge frame, and the sponge plates are equidistantly distributed in the second sponge frame.

[0007] As a preferred solution of the present invention, the adjustable flow chamber comprises a support plate, the support plate is welded and fixed on the side end surface of the partition plate, a first guide frame is welded and fixed on the support plate, a fixing frame is welded and fixed on the bottom of the first guide frame, a threaded rod is threadedly connected to the top of the fixing frame, the bottom end of the threaded rod is rotatably connected to a connecting plate, a push rod is hingedly connected to the bottom end surface of the connecting plate, the bottom end of the push rod is hingedly connected to the first guide plate, a telescopic sleeve rod is welded and fixed on the side end surface of the first guide plate, the other end of the telescopic sleeve rod is welded and fixed to the inner wall of the fixing frame, and a first rubber block and a first rubber plate are fixedly connected to the bottom of the first guide frame. The bottom end of the first rubber block is fixedly connected to the second guide plate, the bottom end of the second guide plate is fixedly connected to the second rubber block, the bottom end of the second rubber block is fixedly connected to the top of the first guide plate, the side end surface of the second guide plate and the side end surface of the first guide plate are fixed to the side end surface of the first rubber plate, four first guide plates and four first rubber plates are provided, the four first rubber plates are alternately distributed with the four first guide plates, the first guide plate corresponds to the second rubber block, the second guide plate, the first rubber block and the telescopic sleeve rod respectively, the first guide plate corresponds to the threaded rod one by one through the connecting plate on the push rod, and the outer diameter of the fixed frame is smaller than the diameter of the first through hole.

[0008] As a preferred solution of the present invention, wherein: a second guide frame is fixedly connected to the inner top surface of the first guide frame, a third rubber block and a second rubber plate are fixedly connected to the bottom end of the second guide frame, a third guide plate is fixedly connected to the bottom end of the third rubber block, a fourth rubber block is fixedly connected to the bottom end of the third guide plate, a fourth guide plate is fixedly connected to the bottom end of the fourth rubber block, a side end surface of the third guide plate and a side end surface of the fourth guide plate are both fixed to the side end surface of the second rubber plate, a fixing rod is fixedly connected to the inner wall of the first guide plate, the other end of the fixing rod is fixedly connected to the outer wall of the fourth guide plate, four fourth guide plates and four second rubber plates are each provided, four fourth guide plates and four second rubber plates are alternately distributed, the fourth guide plate corresponds to the fixing rod, the fourth rubber block, the third guide plate and the third rubber block respectively, and the vertical center line of the first guide frame and the vertical center line of the second guide frame are located on the same vertical line.

[0009] As a preferred scheme of the present invention, wherein: a first liquid pump is installed and fixed on the top side end of the first guide frame, a first connecting pipe is connected to the flange on the first liquid pump, one end of the first connecting pipe is connected to the top of the second guide frame, and the other end of the first connecting pipe is located directly above the placement frame; a second liquid pump is installed and fixed on the inner top surface of the protective shell, a second connecting pipe is connected to the flange on the second liquid pump, one end of the second connecting pipe is connected to a diverter frame, the diverter frame is welded and fixed on the inner top surface of the first guide frame, a diverter hole is opened through the bottom of the diverter frame, the diverter frame is annular as a whole, and the diverter holes are distributed at equal angles at the bottom of the diverter frame.

[0010] As a preferred solution of the present invention, the uniform processing component includes a first storage box, which is welded and fixed on the inner bottom end surface of the protective shell, the top of the first storage box is connected to a first liquid inlet pipe, the first liquid inlet pipe is installed with a first solenoid valve, a servo motor is installed and fixed on the top end surface of the first storage box, a first bevel gear is welded and fixed on the output shaft of the servo motor, a second bevel gear is meshed and connected to the first bevel gear, a sleeve is welded and fixed on the second bevel gear, the sleeve is rotatably connected to the middle part of the first storage box, the top of the sleeve is rotatably connected to the other end of the second connecting pipe through a sealing bearing, a stirring blade is welded and fixed on the sleeve, a fixed tube is welded and fixed on the side end surface of the stirring blade, a first conveying cylinder is fixedly connected to the bottom end of the fixed tube, a first spiral rod is rotatably connected in the first conveying cylinder, a circular gear is welded and fixed to one end of the first spiral rod, a tooth block is welded at an equal angle on the inner bottom end surface of the first storage box, the tooth block is meshed with the circular gear, the first conveying cylinder is distributed at equal angles in the first storage box, and the first conveying cylinder corresponds to the fixed tube one by one.

[0011] As a preferred solution of the present invention, a slot is provided on the side end of the positioning block, a positioning frame is welded and fixed on the bottom end surface of the mounting plate, a return spring is welded and fixed on the side end surface of the positioning frame, a clamping rod is welded and fixed on the other end of the return spring, the clamping rod is slidably connected in the positioning frame, the end of the clamping rod is clamped and connected in the slot, the slots are symmetrically distributed on both sides of the positioning block, the slots correspond to the clamping rods one by one, and the positioning block is slidably connected in the positioning frame.

[0012] As a preferred scheme of the present invention, wherein: the self-cleaning component includes a fixed plate, the fixed plate is welded and fixed on the inner wall of the protective shell, the fixed plate is penetrated by a second through hole, the fixed plate is fixedly connected with a first sponge frame, the fixed plate is fixedly connected with a first conveying pipe, a second conveying pipe and a third conveying pipe, one end of the first conveying pipe is connected to the first sponge frame, the other end of the first conveying pipe and one end of the third conveying pipe are both connected to the second conveying pipe, the other end of the third conveying pipe is connected to a second conveying cylinder, the second conveying cylinder is fixedly connected in a second storage box, the second storage box is welded and fixed on the side end surface of the protective shell, the second conveying cylinder is rotatably connected with a second spiral rod, the length and thickness of the first sponge frame are respectively greater than the diameter and thickness of the first lens, the diameter of the first lens, the diameter of the second lens and the diameter of the filter are the same, the length of the second spiral rod is greater than the length of the second conveying cylinder, and the second conveying cylinder is fixed in the middle part inside the second storage box.

[0013] As a preferred scheme of the present invention, wherein: the top end of the second spiral rod is fixedly connected with a coil, a traction rope is wound on the coil, the traction rope penetrates and is slidably connected to the top side end of the second storage box and the top side end of the protective shell, the end of the traction rope is fixedly connected to the top surface of the mounting plate, a guide wheel is installed on the inner wall of the protective shell, the traction rope is slidably connected to the guide wheel, the top side end of the second storage box is connected with a second liquid inlet pipe, the second liquid inlet pipe is installed with a second solenoid valve, a limiting frame is welded and fixed on the inner bottom end surface of the second storage box, a spiral spring is welded and fixed on the inner wall of the limiting frame, and the inner end of the spiral spring is welded and fixed to the bottom of the second spiral rod.

[0014] A method for using a flow cytometer capable of improving particle resolution comprises the following steps:

[0015] S1: Take samples from cells, tissues or organisms, prepare single cell suspensions, and place them in test tubes, which are then placed in a placement frame in a protective housing;

[0016] S2: Before use, the first lens, the second lens and each filter are automatically cleaned by using a self-cleaning component, and the sheath liquid to be used is uniformly stirred by a uniform processing component;

[0017] S3: Use an adjustable flow chamber to pump and deliver the cell suspension and sheath fluid, and guide the cell suspension through a gradually narrowing channel so that the cell group forms a single cell before passing through the laser at the monitoring point;

[0018] S4: Each cell in the cell suspension moves on the laser beam emitted by the laser, and each cell in the sample is analyzed individually in conjunction with the first lens and the first detector. When each cell passes through the laser beam, the laser beam will be scattered in multiple directions, and the laser beams scattered in multiple directions are collected and detected by the cooperation of each filter and the corresponding second detector.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention is provided with an adjustable flow chamber. By rotating four threaded rods, the corresponding first guide plate can be pushed to move toward the middle or side at the same time through the push rod on the connecting plate. At this time, the first guide plate can push the corresponding fourth guide plate to move synchronously through the fixed rod. In conjunction with the second guide plate and the third guide plate, the diameter of the flow channel can be conveniently adjusted according to the size of the cells. By adjusting the diameter of the flow channel, it can help optimize the flow rate to make it suitable for the analysis requirements of the cells, help reduce cell aggregation, and ensure that cells pass through the analysis area in a single form. The resolution of the flow cytometer in particle analysis can be effectively improved, and the appropriate diameter of the flow channel can reduce the formation of bubbles and avoid interference with the signal.

[0021] 2. The present invention is provided with a uniform processing component, which is driven by a servo motor and can drive the sleeve to rotate stably through the first bevel gear and the second bevel gear. At this time, the sleeve can drive the first conveying cylinder on each fixed tube to perform circular motion through the stirring blade. At the same time, the meshing between the circular gear and the tooth block can drive the first screw rod in the first conveying cylinder to automatically rotate during the revolution, so that the sheath liquid at the bottom of the first storage box can be transported to the top. In this way, the cycle, combined with the continuous stirring of each stirring blade, can be used to efficiently and uniformly stir the sheath liquid in the first storage box, thereby avoiding the problem of uneven distribution of the internal components of the sheath liquid due to stratification or precipitation during long-term storage of the sheath liquid. The use of uniform sheath liquid can reduce the viscosity of the liquid, improve fluidity, and make it easier for cells to pass through the flow cytometer, thereby improving the accuracy of subsequent cell analysis and increasing the diversity and stability of the flow cytometer.

[0022] 3. The present invention is provided with a self-cleaning component. Before starting cell detection, the second hydraulic rod can be used to drive the mounting plate to reciprocate up and down, thereby driving the first lens, the second lens and each filter to move synchronously. At the same time, the mounting plate can pull the traction rope to drive the second spiral rod to rotate automatically, thereby automatically transporting the clean water in the second storage box to each first sponge frame. At this time, each first sponge frame can be used to automatically clean the first lens, the second lens and each filter, thereby avoiding the problem that dust or impurities attached to the first lens, the second lens and each filter affect the transmittance of light, resulting in signal attenuation or distortion, thereby affecting the accuracy of the data. The resolution of the flow cytometer in particle analysis can be further improved.

[0023] 4. The present invention is provided with a card rod and a card slot. By utilizing the cooperation of the card rod and the card slot, combined with the positioning frame and the positioning block, the first lens, the second lens and each filter can be easily disassembled and installed, thereby ensuring the convenience of subsequent replacement of the first lens, the second lens and each filter, thereby increasing the diversity and convenience of use of the flow cytometer.

[0024] 5. The present invention is provided with a collecting box, a second sponge frame and a sponge plate. The collecting box can be used to collect the used sheath fluid and cell suspension as waste liquid. At the same time, with the cooperation of the second sponge frame and each sponge plate, it can effectively avoid sputtering during the waste liquid collection process to cause internal pollution of the equipment or the surrounding environment. In addition, by utilizing the magnetism of the magnetic plate, the collection box can be easily disassembled and installed, thereby ensuring the convenience of subsequent transportation and treatment of the waste liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below in combination with the accompanying drawings and detailed embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0026] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0027] Figure 2 It is a schematic diagram of the connection structure between the second guide plate and the first rubber plate of the present invention;

[0028] Figure 3 This is a schematic diagram of the connection structure between the third guide plate and the second rubber plate of the present invention;

[0029] Figure 4 It is a schematic diagram of the connection structure between the collection box and the sponge frame of the present invention;

[0030] Figure 5 It is a schematic diagram of the connection structure of the sponge frame and the sponge board of the present invention;

[0031] Figure 6 It is a schematic diagram of the main cross-sectional structure of the protective housing of the present invention;

[0032] Figure 7 It is a schematic diagram of the side section structure of the protective housing of the present invention;

[0033] Figure 8 It is a schematic diagram of the main cross-sectional structure of the first storage box of the present invention;

[0034] Fig. 9 It is a schematic diagram of the main cross-sectional structure of the casing of the present invention;

[0035] Fig.10 is a schematic diagram of a top-sectional structure of a first storage box of the present invention;

[0036] Fig.11 It is a schematic diagram of the main cross-sectional structure of the first guide frame of the present invention;

[0037] Fig.12 The present invention Fig.11 The enlarged structural diagram at A in the middle;

[0038] Fig.13 It is a schematic diagram of the structure of the push rod of the present invention when viewed from above;

[0039] Fig.14 It is a schematic diagram of the top cross-sectional structure of the diverter frame of the present invention;

[0040] Fig.15 It is a schematic diagram of the main cross-sectional structure of the second storage box of the present invention;

[0041] Fig.16 It is a schematic diagram of the top view of the spiral spring of the present invention;

[0042] Fig.17 It is a schematic diagram of the main cross-sectional structure of the positioning block of the present invention;

[0043] Fig.18 It is a schematic diagram of the top-sectional structure of the protective housing of the present invention;

[0044] Fig.19 This is a schematic diagram of the structure of the mounting plate of the present invention when viewed from above;

[0045] Fig. 20 It is a schematic diagram of the top view structure of the first sponge frame of the present invention.

[0046] In the figure: 1, protective housing; 2, partition plate; 3, first door plate; 4, second door plate; 5, first hydraulic rod; 6, placement frame; 7, test tube; 8, adjustable flow chamber; 801, support plate; 802, first flow guide frame; 803, fixed frame; 804, threaded rod; 805, connecting plate; 806, push rod; 807, first flow guide plate; 808, telescopic sleeve rod; 809, first rubber block; 810, second flow guide plate; 811, second rubber block; 812, first rubber plate; 813, second flow guide frame; 814, third rubber block; 815, third guide plate; 816, fourth rubber block; 817, fourth guide plate; 818, second rubber plate; 819, fixing rod; 820, first liquid pump; 821, first connecting pipe; 822, second liquid pump; 823, second connecting pipe; 824, diversion frame; 825, guide hole; 9, uniform treatment component; 901, first storage box; 902, first liquid inlet pipe; 903, first solenoid valve; 904, servo motor; 905, first bevel gear; 906, second bevel gear; 907, sleeve; 908, stirring blade; 909, fixed tube; 910, first conveying cylinder; 911, first spiral rod; 912, circular gear; 913, gear block; 10, support plate; 11, second hydraulic rod; 12, mounting plate; 13, first through hole; 14, first lens; 15, second lens; 16, filter; 17, positioning block; 18, slot; 19, positioning frame; 20, reset spring; 21, clamping rod; 22, laser; 23, first detector; 24, second detector; 25, self-cleaning assembly; 2501, fixed plate; 2502, second Through hole; 2503, first sponge frame; 2504, first delivery pipe; 2505, second delivery pipe; 2506, third delivery pipe; 2507, second delivery cylinder; 2508, second spiral rod; 2509, coil; 2510, traction rope; 2511, guide wheel; 2512, second storage box; 2513, second liquid inlet pipe; 2514, second solenoid valve; 2515, limit frame; 2516, volute spring; 26, collection box; 27, magnetic plate; 28, second sponge frame; 29, sponge plate; 30, control panel. DETAILED DESCRIPTION

[0047] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0048] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0049] Secondly, the present invention is described in detail with reference to schematic diagrams. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0050] Example

[0051] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0052] like Figure 1-20As shown, a flow cytometer capable of improving particle resolution comprises a protective housing 1, a first lens 14, a second lens 15 and a filter 16, a partition plate 2 is welded and fixed inside the protective housing 1, a first door plate 3 and a second door plate 4 are installed on the protective housing 1, a first hydraulic rod 5 is installed at the bottom end of the protective housing 1, a placement frame 6 is fixedly connected to the top end of the first hydraulic rod 5, a test tube 7 is arranged in the placement frame 6, an adjustable flow chamber 8 is installed in the protective housing 1, a uniform processing component 9 is installed in the protective housing 1, a support plate 10 is welded and fixed on the inner wall of the protective housing 1 and the side end surface of the partition plate 2, and a A second hydraulic rod 11, the bottom end of the second hydraulic rod 11 is fixedly connected to a mounting plate 12, a first through hole 13 is penetrated through the mounting plate 12, the tops of the first lens 14, the second lens 15 and the filter 16 are fixedly connected to a positioning block 17, a laser 22 is fixedly installed on the side end surface of the partition plate 2, a first detector 23 and a second detector 24 are fixedly installed on the inner wall of the protective shell 1, a self-cleaning component 25 is installed in the protective shell 1, a collecting box 26 is slidably connected to the side end of the bottom of the protective shell 1, a magnetic plate 27 is fixedly connected to the side end surface of the collecting box 26, and the magnetic plate 27 is magnetically adsorbed and connected to the bottom of the protective shell 1 On the side end surface of the part, a second sponge frame 28 is arranged in the collection box 26, and a sponge plate 29 is fixedly connected in the second sponge frame 28. A control panel 30 is fixedly installed on the side end surface of the protective shell 1. The adjustable flow chamber 8 can be used to conveniently adjust the diameter of the flow channel according to the size of the cells. By adjusting the diameter of the flow channel, it can help optimize the flow rate to make it suitable for the analysis requirements of the cells, help reduce cell aggregation, and ensure that the cells pass through the analysis area in a single form, which can effectively improve the resolution of the flow cytometer in particle analysis, and cooperate with the uniform processing component 9 to efficiently and uniformly stir the sheath fluid to be used. , which avoids the problem of uneven distribution of components inside the sheath fluid due to stratification or precipitation during long-term storage of the sheath fluid. The use of uniform sheath fluid can reduce the viscosity of the liquid, improve fluidity, and make it easier for cells to pass through the flow cytometer, thereby improving the accuracy of subsequent cell analysis. In addition, the use of the self-cleaning component 25 can automatically clean the first lens 14, the second lens 15 and each filter 16, avoiding the problem that attached dust or impurities affect the transmittance of light, causing signal attenuation or distortion, thereby affecting the accuracy of the data, and can further improve the resolution of the flow cytometer in particle analysis.

[0053] In this embodiment, the outer wall of the test tube 7 is in contact with the inner wall of the placement frame 6, the top surface of the support plate 10 is in contact with the bottom surface of the mounting plate 12, four second hydraulic rods 11 are provided, and the four second hydraulic rods 11 are evenly distributed around the mounting plate 12, the second lens 15 is symmetrically distributed on the front and back sides of the protective shell 1, the filter 16 is equidistantly distributed on the front and back sides of the protective shell 1, the filter 16 is inclined, and the filter 16 corresponds to the second detector 24 one by one, the collecting box 26 is in contact with the inner wall of the protective shell 1, and the inner wall of the collecting box 26 is in contact with the outer wall of the second sponge frame 28, and the sponge plates 29 are equidistantly distributed in the second sponge frame 28. The collecting box 26 can be used to collect the used sheath fluid and cell suspension for waste liquid collection. At the same time, with the cooperation of the second sponge frame 28 and each sponge plate 29, it can effectively avoid sputtering during the waste liquid collection process, resulting in internal pollution of the equipment or pollution of the surrounding environment.

[0054] In this embodiment, the adjustable flow chamber 8 includes a support plate 801, which is welded and fixed on the side end surface of the partition plate 2, a first guide frame 802 is welded and fixed on the support plate 801, a fixed frame 803 is welded and fixed on the bottom of the first guide frame 802, a threaded rod 804 is threadedly connected to the top of the fixed frame 803, and a connecting plate 805 is rotatably connected to the bottom end of the threaded rod 804, a push rod 806 is hinged on the bottom end surface of the connecting plate 805, and a first guide plate 807 is hinged on the bottom end of the push rod 806, a telescopic sleeve rod 808 is welded and fixed on the side end surface of the first guide plate 807, and the other end of the telescopic sleeve rod 808 is welded and fixed on the inner wall of the fixed frame 803, and a first rubber block 809 and a first The bottom end of the first rubber block 809 is fixedly connected to the second guide plate 810, the bottom end of the second guide plate 810 is fixedly connected to the second rubber block 811, the bottom end of the second rubber block 811 is fixedly connected to the top of the first guide plate 807, the side end surface of the second guide plate 810 and the side end surface of the first guide plate 807 are fixed to the side end surface of the first rubber plate 812, the first guide plate 807 and the first rubber plate 812 are each provided with four, the four first rubber plates 812 are alternately distributed with the four first guide plates 807, the first guide plate 807 corresponds to the second rubber block 811, the second guide plate 810, the first rubber block 809 and the telescopic sleeve rod 808 respectively, the first guide plate 807 is connected to the first guide plate 807 through the connecting plate 8 on the push rod 806 05 corresponds to the threaded rod 804 one by one, the outer diameter of the fixing frame 803 is smaller than the diameter of the first through hole 13, the inner top surface of the first guide frame 802 is fixedly connected to the second guide frame 813, the bottom end of the second guide frame 813 is fixedly connected to the third rubber block 814 and the second rubber plate 818, the bottom end of the third rubber block 814 is fixedly connected to the third guide plate 815, the bottom end of the third guide plate 815 is fixedly connected to the fourth rubber block 816, the bottom end of the fourth rubber block 816 is fixedly connected to the fourth guide plate 817, the side end surface of the third guide plate 815 and the side end surface of the fourth guide plate 817 are both fixed to the side end surface of the second rubber plate 818, the inner wall of the first guide plate 807 is fixedly connected to the fixing rod 819, and the other end of the fixing rod 819 is fixedly connected to the second rubber plate 818. The first end is fixedly connected to the outer wall of the fourth guide plate 817, and the fourth guide plate 817 and the second rubber plate 818 are each provided with four, and the four fourth guide plates 817 and the four second rubber plates 818 are distributed alternately. The fourth guide plate 817 corresponds to the fixing rod 819, the fourth rubber block 816, the third guide plate 815 and the third rubber block 814 one by one, respectively. The vertical center line of the first guide frame 802 and the vertical center line of the second guide frame 813 are located on the same vertical line. By rotating the four threaded rods 804, the push rod 806 on the connecting plate 805 can push the corresponding first guide plate 807 to move to the middle or side at the same time. At this time, the first guide plate 807 can push the corresponding fourth guide plate 817 to move synchronously through the fixing rod 819.In conjunction with the second guide plate 810 and the third guide plate 815, the diameter of the flow channel can be conveniently adjusted according to the size of the cells.

[0055] In this embodiment, a first liquid pump 820 is fixedly installed on the top side end of the first guide frame 802, and a first connecting pipe 821 is flange-connected on the first liquid pump 820, one end of the first connecting pipe 821 is connected to the top of the second guide frame 813, and the other end of the first connecting pipe 821 is located directly above the placement frame 6, and a second liquid pump 822 is fixedly installed on the inner top surface of the protective shell 1, and a second connecting pipe 823 is flange-connected on the second liquid pump 822, and one end of the second connecting pipe 823 is connected to a diverter frame 824, which is welded and fixed on the inner top surface of the first guide frame 802, and a diverter hole 825 is opened through the bottom of the diverter frame 824, and the diverter frame 824 is annular as a whole, and the diverter holes 825 are equiangularly distributed on the bottom of the diverter frame 824, and the diverter holes 825 on the diverter frame 824 can ensure that the sheath liquid can be evenly distributed in the first guide frame 802 during the suction and transportation process, thereby ensuring the stability of the subsequent transportation and flow state of the sheath liquid.

[0056] In this embodiment, the uniform processing component 9 includes a first storage box 901, which is welded and fixed on the inner bottom end surface of the protective shell 1, and the top of the first storage box 901 is connected to a first liquid inlet pipe 902, and a first solenoid valve 903 is installed on the first liquid inlet pipe 902. A servo motor 904 is installed and fixed on the top end surface of the first storage box 901, and a first bevel gear 905 is welded and fixed on the output shaft of the servo motor 904. The first bevel gear 905 is meshed and connected with a second bevel gear 906, and the second bevel gear 906 is meshed and connected. A sleeve 907 is welded and fixed on the top, and the sleeve 907 is rotatably connected to the middle part of the first storage box 901. The top of the sleeve 907 is rotatably connected to the other end of the second connecting pipe 823 through a sealed bearing. A stirring blade 908 is welded and fixed on the sleeve 907. A fixed pipe 909 is welded and fixed on the side end surface of the stirring blade 908. The bottom end of the fixed pipe 909 is fixedly connected to a first conveying cylinder 910. A first spiral rod 911 is rotatably connected in the first conveying cylinder 910. A circular gear 912 is welded and fixed to one end of the first spiral rod 911. A tooth block 913 is welded at an equal angle on the inner bottom end surface of the storage box 901, and the tooth block 913 is meshed with the circular gear 912. The first conveying cylinder 910 is distributed at an equal angle in the first storage box 901. The first conveying cylinder 910 corresponds to the fixed tube 909 one by one. The servo motor 904 drives the sleeve 907 to rotate stably through the first bevel gear 905 and the second bevel gear 906. At this time, the sleeve 907 can drive the first conveying cylinder 910 on each fixed tube 909 to perform a circular motion through the stirring blade 908. At the same time, The meshing between the circular gear 912 and the tooth block 913 can drive the first screw rod 911 in the first conveying cylinder 910 to automatically rotate during the revolution, thereby transporting the sheath liquid at the bottom of the first storage box 901 to the top, and so on. In combination with the continuous stirring of each stirring blade 908, the sheath liquid in the first storage box 901 can be efficiently and evenly stirred. The use of uniform sheath liquid can reduce the viscosity of the liquid, improve fluidity, make it easier for cells to pass through the flow cytometer, and thus improve the accuracy of subsequent cell analysis.

[0057] In this embodiment, a slot 18 is provided at the side end of the positioning block 17, a positioning frame 19 is welded and fixed on the bottom end surface of the mounting plate 12, a return spring 20 is welded and fixed on the side end surface of the positioning frame 19, and a clamping rod 21 is welded and fixed at the other end of the return spring 20, the clamping rod 21 is slidably connected in the positioning frame 19, and the end of the clamping rod 21 is clamped and connected in the slot 18, the slots 18 are symmetrically distributed on both sides of the positioning block 17, and the slots 18 correspond to the clamping rod 21 one by one, and the positioning block 17 is slidably connected in the positioning frame 19. By utilizing the cooperation of the clamping rod 21 and the slot 18, combined with the positioning frame 19 and the positioning block 17, the disassembly and installation of the first lens 14, the second lens 15 and each filter 16 can be conveniently completed, thereby ensuring the convenience of subsequent replacement of the first lens 14, the second lens 15 and each filter 16.

[0058] In this embodiment, the self-cleaning component 25 includes a fixed plate 2501, which is welded and fixed to the inner wall of the protective shell 1. A second through hole 2502 is opened on the fixed plate 2501. A first sponge frame 2503 is fixedly connected to the fixed plate 2501. A first delivery pipe 2504, a second delivery pipe 2505 and a third delivery pipe 2506 are fixedly connected in the fixed plate 2501. One end of the first delivery pipe 2504 is connected to the first sponge frame 2503, and the other end of the first delivery pipe 2504 and one end of the third delivery pipe 2506 are both connected to the second delivery pipe 2505. The other end of the third delivery pipe 2506 is connected to the second delivery tube 2507, the second conveying cylinder 2507 is fixedly connected in the second storage box 2512, the second storage box 2512 is welded and fixed on the side end surface of the protective shell 1, the second conveying cylinder 2507 is rotatably connected with the second spiral rod 2508, the length and thickness of the first sponge frame 2503 are respectively greater than the diameter and thickness of the first lens 14, the diameter of the first lens 14, the diameter of the second lens 15 and the diameter of the filter 16 are the same, the length of the second spiral rod 2508 is greater than the length of the second conveying cylinder 2507, the second conveying cylinder 2507 is fixed in the middle part of the second storage box 2512, and the top of the second spiral rod 2508 is fixedly connected with the coil 2509 A traction rope 2510 is wound around the coil 2509, and the traction rope 2510 is slidably connected to the top side end of the second storage box 2512 and the top side end of the protective shell 1. The end of the traction rope 2510 is fixedly connected to the top surface of the mounting plate 12, and a guide wheel 2511 is installed on the inner wall of the protective shell 1. The traction rope 2510 is slidably connected to the guide wheel 2511. The top side end of the second storage box 2512 is connected to a second liquid inlet pipe 2513, and a second solenoid valve 2514 is installed on the second liquid inlet pipe 2513. A limiting frame 2515 is welded and fixed on the inner bottom end surface of the second storage box 2512, and a limiting frame 2515 is welded and fixed on the inner wall of the limiting frame 2515. The spiral spring 2516, the inner end of the spiral spring 2516 is welded and fixed to the bottom of the second spiral rod 2508, and the second hydraulic rod 11 is used to drive the mounting plate 12 to reciprocate up and down, thereby driving the first lens 14, the second lens 15 and each filter 16 to move synchronously. At the same time, the mounting plate 12 pulls the traction rope 2510 to drive the second spiral rod 2508 to rotate automatically, thereby automatically transporting the clean water in the second storage box 2512 to each first sponge frame 2503. At this time, each first sponge frame 2503 can be used to automatically clean the first lens 14, the second lens 15 and each filter 16.

[0059] It should be noted that the present invention is a flow cytometer capable of improving particle resolution and a method for using the same. First, the staff member takes a sample from a cell, tissue or organism, and after preparing a single cell suspension, the sample can be transported into a test tube 7. At this time, the staff member can open the first door panel 3 on the protective housing 1, and place the test tube 7 in the placement frame 6. Then, by driving the first hydraulic rod 5, the placement frame 6 can be pushed upward, and the test tube 7 can be pushed upward, so that the first connecting tube 821 can be stably inserted into the test tube 7.

[0060] Then the staff can drive the servo motor 904 on the first storage box 901 and the second hydraulic rod 11 in the protective shell 1. Under the driving action of the servo motor 904, the second bevel gear 906 can be driven to rotate through the first bevel gear 905 on the output shaft, and then the sleeve 907 can be driven to rotate stably in the first storage box 901. At this time, under the continuous rotation of the sleeve 907, the stirring blade 908 can drive each fixed tube 909 to perform circular motion, and then the corresponding first conveying cylinder 910 of each fixed tube 909 can be driven to perform synchronous circular motion. During the movement of each first conveying cylinder 910, the circular gear 912 on the internal first spiral rod 911 can be driven to perform circular motion on the tooth block 913, that is, revolution. During the revolution of the circular gear 912, the meshing with the tooth block 913 can drive the circular gear 912 to automatically rotate during the revolution, and then drive the first screw rod 911 to automatically rotate inside the first conveying cylinder 910 during the revolution. At this time, under the continuous rotation of the first screw rod 911, the sheath liquid at the bottom of the first storage box 901 can be sucked through the first conveying cylinder 910, and the sheath liquid at the bottom can be transported to the top in cooperation with the fixed tube 909. By utilizing the circulation of the sheath liquid and the continuous stirring of each stirring blade 908, the sheath liquid in the first storage box 901 can be efficiently and evenly stirred, thereby avoiding the problem of stratification or precipitation of the sheath liquid during long-term storage, which leads to uneven distribution of the internal components of the sheath liquid.

[0061] At the same time, under the driving action of the second hydraulic rod 11, the mounting plate 12 can be driven to move upward first and then downward. When the mounting plate 12 moves downward, under the limiting action of the support plate 10, it can ensure that the mounting plate 12 can be easily and accurately reset to avoid movement deviation affecting the accuracy of subsequent detection work; when the mounting plate 12 moves upward, it can drive the first lens 14, the second lens 15 and each filter 16 installed in the card-engaged state to move upward synchronously until they move to above the first sponge frame 2503. At the same time, under the movement action of the mounting plate 12, the end of the traction rope 2510 can be driven to move upward synchronously. At this time, the spiral spring 2516 inside the limit frame 2515 Under the elastic action of the second screw rod 2508 in the second conveying cylinder 2507, the second screw rod 2508 can automatically rotate. At the same time, the second screw rod 2508 can automatically reel in the traction rope 2510 through the coil 2509 at the top to ensure the stability of the subsequent repeated working state of the traction rope 2510. At this time, under the automatic rotation of the second screw rod 2508, the clean water in the second storage box 2512 can be sucked and transported to the third conveying pipe 2506 through the second conveying cylinder 2507. Then, the clean water can be automatically transported to each first conveying pipe 2504 through the second conveying pipe 2505, and then can be transported to each first sponge frame 2503 to moisten the first sponge frame 2503;

[0062] Then, the second hydraulic rod 11 pushes the mounting plate 12 to move downward and reset. Under the downward action of the mounting plate 12, the traction rope 2510 on the guide wheel 2511 can be pulled. At this time, under the pulling action of the traction rope 2510, the second spiral rod 2508 can be driven to rotate in the opposite direction through the coil 2509. At this time, the spiral feeding direction of the second spiral rod 2508 is opposite, and the clean water will not be transported further. However, at this time, the clean water has been absorbed inside each first sponge frame 2503, which is enough for use. At this time, under the movement of the mounting plate 12, the first lens 14 installed in the card can be driven The second lens 15 and each filter 16 move downward synchronously to reset, and in the process of their movement, they can pass through each first sponge frame 2503. Under the action of each first sponge frame 2503, the dust and impurities attached to the end faces of the first lens 14, the second lens 15 and each filter 16 can be automatically cleaned, so as to avoid the dust or impurities attached to the first lens 14, the second lens 15 and each filter 16 affecting the transmittance of light, causing signal attenuation or distortion, thereby affecting the accuracy of data, and thus effectively improving the resolution of the flow cytometer in particle analysis;

[0063] Then the staff can adjust the bottom flow diameter of the first guide frame 802 and the bottom flow diameter of the second guide frame 813 according to the cell size. The staff can rotate the four threaded rods 804 on the fixed frame 803. Under the rotation of the threaded rods 804, the connecting plate 805 can be pushed to move downward. At the same time, under the limiting and guiding action of the telescopic sleeve rod 808, each threaded rod 804 can push the corresponding first guide plate 807 to move to the middle or side at the same time through the push rod 806 on the connecting plate 805. Under the movement of each first guide plate 807, the first rubber block 809 and the second rubber block 811 can adjust the inclination angle of the second guide plate 810. The first guide plate 807 is used to adjust the bottom flow diameter of the first guide frame 802 in cooperation with the alternate first rubber plates 812 under the movement of the first guide plate 807; similarly, during the movement of each first guide plate 807, the corresponding fourth guide plate 817 can be pushed to move toward the middle or the side at the same time through the fixing rod 819, and under the movement of each fourth guide plate 817, the third rubber block 814 and the fourth rubber block 816 can be used to adjust the inclination angle of the third guide plate 815, and under the movement of each fourth guide plate 817, the alternate second rubber plates 818 can be used to synchronously adjust the bottom flow diameter of the second guide frame 813;

[0064] Then the staff can drive the first liquid pump 820 and the second liquid pump 822. Under the driving action of the first liquid pump 820, the cell suspension in the test tube 7 can be sucked through the first connecting tube 821. At the same time, under the driving action of the second liquid pump 822, the sheath liquid inside the first storage box 901 can be sucked through the second connecting tube 823 and the sleeve 907. Before the sheath liquid is sucked, the stirring work of the sheath liquid can be stopped. At this time, under the suction and delivery action of the sheath liquid, the second connecting tube 823 can The sheath liquid is transported to the diversion frame 824, and then the diversion frame 824 can be uniformly transported to the first guide frame 802 through the various guide holes 825 at the bottom. At the same time, under the suction and transport action of the first connecting tube 821, the cell suspension can be transported to the second guide frame 813. At this time, under the synchronous flow action of the cell suspension and the sheath liquid, the sheath liquid can create a laminar flow environment, reducing the friction between the cells and the inner wall of the flow channel, so that the cells can stably pass through the detection area in a single form to avoid aggregation and mutual interference;

[0065] When the cell suspension flows downward, individual cells in the cell suspension can pass through the laser beam generated by the laser 22 one by one. After irradiating the cells, the forward scattered light is irradiated onto the first detector 23 through the first lens 14. Similarly, the side scattered light is irradiated onto the filter 16 through the second lenses 15 on the front and rear sides, and then reflected to the corresponding second detector 24. By collecting and detecting the side scattered light on the front and rear sides, the detection accuracy can be further improved. The collected optical signals are converted into electrical signals, and after computer analysis, the characteristic data of the cells, such as quantity, size, fluorescence intensity, etc., are generated.

[0066] The waste liquid after detection can automatically fall into the collection box 26, and the collection box 26 can be used to collect the used sheath liquid and cell suspension. At the same time, with the cooperation of the second sponge frame 28 and each sponge plate 29, it can effectively avoid the occurrence of sputtering in the process of waste liquid collection, which may cause internal pollution of the equipment or pollution of the surrounding environment. After the detection work is completed, the staff only needs to pull the magnetic plate 27 outward until the magnetic plate 27 moves away from the protective shell 1. At this time, the magnetic plate 27 can drive the collection box 26 to move away from the protective shell 1, thereby ensuring the convenience of subsequent transportation and processing of the waste liquid, and the staff can take out the second sponge frame 28 for subsequent cleaning. The collection box 26 after cleaning can be stably inserted into the protective shell 1. The magnetism of the magnetic plate 27 can be used to conveniently complete the magnetic suction installation of the collection box 26, thereby ensuring the stability of the subsequent working state of the collection box 26.

[0067] The staff can open the second door panel 4 on the protective housing 1, and then pull the lever 21 on the positioning frame 19 outward until the lever 21 moves out of the slot 18 on the positioning block 17. At this time, the positioning block 17 can move out of the positioning frame 19, thereby conveniently completing the disassembly of the first lens 14, the second lens 15 or each filter 16; similarly, when it is necessary to engage and install the first lens 14, the second lens 15 or each filter 16, it is only necessary to pull the lever 21 on the positioning frame 19 outward again. When the positioning block 17 on the first lens 14, the second lens 15 or each filter 16 is inserted into the positioning frame 19, the staff can then release the clamping rod 21. At this time, under the elastic action of the reset spring 20, the clamping rod 21 can be driven to rotate and engage in the clamping groove 18 on the positioning block 17, so that the first lens 14, the second lens 15 or each filter 16 can be easily installed. By easily completing the replacement of the first lens 14, the second lens 15 or each filter 16, the service life of the flow cytometer can be effectively improved.

[0068] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A flow cytometer capable of improving particle resolution, comprising a protective housing (1), a first lens (14), a second lens (15) and a filter (16), characterized in that: A partition plate (2) is welded and fixed inside the protective shell (1), a first door plate (3) and a second door plate (4) are installed on the protective shell (1), a first hydraulic rod (5) is installed at the bottom end of the protective shell (1), a placement frame (6) is fixedly connected to the top end of the first hydraulic rod (5), a test tube (7) is arranged in the placement frame (6), an adjustable flow chamber (8) is installed in the protective shell (1), a uniform processing component (9) is installed in the protective shell (1), a support plate (10) is welded and fixed on the inner wall of the protective shell (1) and the side end surface of the partition plate (2), a second hydraulic rod (11) is installed at the top end of the protective shell (1), a mounting plate (12) is fixedly connected to the bottom end of the second hydraulic rod (11), a first through hole (13) is opened through the mounting plate (12), and the first lens ( 14), the top ends of the second lens (15) and the filter (16) are fixedly connected with a positioning block (17), a laser (22) is fixedly mounted on the side end surface of the partition plate (2), a first detector (23) and a second detector (24) are fixedly mounted on the inner wall of the protective shell (1), a self-cleaning component (25) is installed in the protective shell (1), a collecting box (26) is slidably connected through the bottom side end of the protective shell (1), a magnetic plate (27) is fixedly connected to the side end surface of the collecting box (26), and the magnetic plate (27) is magnetically adsorbed and connected to the bottom side end surface of the protective shell (1), a second sponge frame (28) is arranged in the collecting box (26), a sponge plate (29) is fixedly connected in the second sponge frame (28), and a control panel (30) is fixedly mounted on the side end surface of the protective shell (1).

2. A flow cytometer capable of improving particle resolution according to claim 1, characterized in that: The outer wall of the test tube (7) is in contact with the inner wall of the placement frame (6); the top surface of the support plate (10) is in contact with the bottom surface of the mounting plate (12); four second hydraulic rods (11) are provided, and the four second hydraulic rods (11) are evenly distributed around the mounting plate (12); the second lenses (15) are symmetrically distributed on the front and back sides of the protective shell (1); the optical filters (16) are equidistantly distributed on the front and back sides of the protective shell (1); the optical filters (16) are inclined; the optical filters (16) correspond one to one with the second detector (24); the collection box (26) is in contact with the inner wall of the protective shell (1); the inner wall of the collection box (26) is in contact with the outer wall of the second sponge frame (28); and the sponge plates (29) are equidistantly distributed in the second sponge frame (28).

3. A flow cytometer capable of improving particle resolution according to claim 1, characterized in that: The adjustable flow chamber (8) comprises a support plate (801), wherein the support plate (801) is welded and fixed on the side end surface of the partition plate (2), a first flow guide frame (802) is welded and fixed on the support plate (801), a fixing frame (803) is welded and fixed on the bottom of the first flow guide frame (802), a threaded rod (804) is threadedly connected to the top of the fixing frame (803), a connecting plate (805) is rotatably connected to the bottom of the threaded rod (804), and the connecting plate A push rod (806) is hinged on the bottom end surface of the first guide frame (805), and a first guide plate (807) is hinged on the bottom end of the push rod (806). A telescopic sleeve rod (808) is welded and fixed on the side end surface of the first guide plate (807), and the other end of the telescopic sleeve rod (808) is welded and fixed on the inner wall of the fixed frame (803). A first rubber block (809) and a first rubber plate (812) are fixedly connected to the bottom of the first guide frame (802). The first rubber block (809) The bottom end of the second guide plate (810) is fixedly connected to the second guide plate (810), the bottom end of the second guide plate (810) is fixedly connected to the second rubber block (811), the bottom end of the second rubber block (811) is fixedly connected to the top end of the first guide plate (807), the side end surface of the second guide plate (810) and the side end surface of the first guide plate (807) are both fixed to the side end surface of the first rubber plate (812), and the first guide plate (807) and the first rubber plate (812) are both provided with four The four first rubber plates (812) and the four first guide plates (807) are alternately distributed, the first guide plates (807) respectively correspond to the second rubber block (811), the second guide plate (810), the first rubber block (809) and the telescopic sleeve rod (808), the first guide plates (807) correspond to the threaded rod (804) via the connecting plates (805) on the push rod (806), and the outer diameter of the fixing frame (803) is smaller than the diameter of the first through hole (13).

4. A flow cytometer capable of improving particle resolution according to claim 3, characterized in that: A second flow guide frame (813) is fixedly connected to the inner top surface of the first flow guide frame (802); a third rubber block (814) and a second rubber plate (818) are fixedly connected to the bottom end of the second flow guide frame (813); a third flow guide plate (815) is fixedly connected to the bottom end of the third rubber block (814); a fourth rubber block (816) is fixedly connected to the bottom end of the third flow guide plate (815); a fourth flow guide plate (817) is fixedly connected to the bottom end of the fourth rubber block (816); side end surfaces of the third flow guide plate (815) and the fourth flow guide plate (817) are both fixed to the side end surfaces of the second rubber plate (818); and the first A fixing rod (819) is fixedly connected to the inner wall of the guide plate (807), and the other end of the fixing rod (819) is fixedly connected to the outer wall of the fourth guide plate (817). Four fourth guide plates (817) and four second rubber plates (818) are provided, and the four fourth guide plates (817) and the four second rubber plates (818) are alternately distributed. The fourth guide plates (817) correspond to the fixing rod (819), the fourth rubber block (816), the third guide plates (815) and the third rubber block (814) respectively, and the vertical center line of the first guide frame (802) and the vertical center line of the second guide frame (813) are located on the same vertical line.

5. A flow cytometer capable of improving particle resolution according to claim 4, characterized in that: A first liquid pump (820) is fixedly installed on the top side end of the first flow guide frame (802); a first connecting pipe (821) is flange-connected to the first liquid pump (820); one end of the first connecting pipe (821) is connected to the top of the second flow guide frame (813); the other end of the first connecting pipe (821) is located directly above the placement frame (6); a second liquid pump (822) is fixedly installed on the inner top surface of the protective shell (1); a second connecting pipe (823) is flange-connected to the second liquid pump (822); one end of the second connecting pipe (823) is connected to a diversion frame (824); the diversion frame (824) is welded and fixedly installed on the inner top surface of the first flow guide frame (802); a diversion hole (825) is opened through the bottom of the diversion frame (824); the diversion frame (824) is annular as a whole; the diversion holes (825) are evenly distributed at the bottom of the diversion frame (824).

6. A flow cytometer capable of improving particle resolution according to claim 5, characterized in that: The uniform processing assembly (9) comprises a first storage box (901), the first storage box (901) is welded and fixed on the inner bottom end surface of the protective shell (1), the top of the first storage box (901) is connected to a first liquid inlet pipe (902), the first liquid inlet pipe (902) is installed with a first solenoid valve (903), the top surface of the first storage box (901) is installed with a servo motor (904), the output shaft of the servo motor (904) is welded and fixed with a first bevel gear (905), the first bevel gear (905) is meshingly connected with a second bevel gear (906), the second bevel gear (906) is welded and fixed with a sleeve (907), the sleeve (907) is rotatably connected to the middle part of the first storage box (901), the top of the sleeve (907) is fixed with a first bevel gear (905) and the sleeve (907) is rotatably connected to the inner middle part of the first storage box (901). The sleeve (907) is rotatably connected to the other end of the second connecting tube (823) through a sealing bearing. A stirring blade (908) is welded and fixed on the sleeve (907). A fixed tube (909) is welded and fixed on the side end surface of the stirring blade (908). The bottom end of the fixed tube (909) is fixedly connected to a first conveying cylinder (910). A first spiral rod (911) is rotatably connected inside the first conveying cylinder (910). A circular gear (912) is welded and fixed to one end of the first spiral rod (911). A tooth block (913) is welded at an equal angle on the inner bottom end surface of the first storage box (901). The tooth block (913) meshes with the circular gear (912). The first conveying cylinders (910) are distributed at equal angles inside the first storage box (901). The first conveying cylinders (910) correspond one to one with the fixed tube (909).

7. A flow cytometer capable of improving particle resolution according to claim 1, characterized in that: A slot (18) is provided at the side end of the positioning block (17); a positioning frame (19) is welded and fixed on the bottom end surface of the mounting plate (12); a return spring (20) is welded and fixed on the side end surface of the positioning frame (19); a clamping rod (21) is welded and fixed at the other end of the return spring (20); the clamping rod (21) is slidably connected in the positioning frame (19); the end of the clamping rod (21) is clamped and connected in the slot (18); the slots (18) are symmetrically distributed on both sides of the positioning block (17); the clamping slots (18) correspond to the clamping rod (21) one by one; and the positioning block (17) is slidably connected in the positioning frame (19).

8. A flow cytometer capable of improving particle resolution according to claim 1, characterized in that: The self-cleaning component (25) comprises a fixing plate (2501), wherein the fixing plate (2501) is welded and fixed to the inner wall of the protective shell (1), a second through hole (2502) is penetrated through the fixing plate (2501), a first sponge frame (2503) is fixedly connected to the fixing plate (2501), a first delivery pipe (2504), a second delivery pipe (2505) and a third delivery pipe (2506) are fixedly connected inside the fixing plate (2501), one end of the first delivery pipe (2504) is connected to the first sponge frame (2503), the other end of the first delivery pipe (2504) and one end of the third delivery pipe (2506) are both connected to the second delivery pipe (2505), and the third delivery pipe (2506) is fixedly connected to the fixing plate (2501). The other end is connected to a second conveying cylinder (2507), which is fixedly connected to a second storage box (2512). The second storage box (2512) is welded and fixed to the side end surface of the protective shell (1). A second spiral rod (2508) is rotatably connected to the second conveying cylinder (2507). The length and thickness of the first sponge frame (2503) are respectively greater than the diameter and thickness of the first lens (14). The diameter of the first lens (14), the diameter of the second lens (15) and the diameter of the filter (16) are the same. The length of the second spiral rod (2508) is greater than the length of the second conveying cylinder (2507). The second conveying cylinder (2507) is fixed to the middle part inside the second storage box (2512).

9. A flow cytometer capable of improving particle resolution according to claim 8, characterized in that: The top end of the second spiral rod (2508) is fixedly connected to a coil (2509), and a traction rope (2510) is wound around the coil (2509). The traction rope (2510) passes through and is slidably connected to the top side end of the second storage box (2512) and the top side end of the protective shell (1). The end of the traction rope (2510) is fixedly connected to the top surface of the mounting plate (12). A guide wheel (2511) is installed on the inner wall of the protective shell (1). The traction rope (2510) is slidably connected to the top side end of the second storage box (2512). On the guide wheel (2511), the top side end of the second storage box (2512) is connected to a second liquid inlet pipe (2513), and a second solenoid valve (2514) is installed on the second liquid inlet pipe (2513). A limit frame (2515) is welded and fixed on the inner bottom end surface of the second storage box (2512), and a spiral spring (2516) is welded and fixed on the inner wall of the limit frame (2515), and the inner end of the spiral spring (2516) is welded and fixed to the bottom of the second spiral rod (2508).

10. A method for using a flow cytometer capable of improving particle resolution, using the flow cytometer capable of improving particle resolution as claimed in claim 1, characterized in that: The following steps are involved: S1: taking a sample from a cell, tissue or organism, preparing a single cell suspension, and placing it in a test tube (7), and then placing the test tube (7) in a placement frame (6) in a protective housing (1); S2: Before use, the first lens (14), the second lens (15) and each filter (16) are automatically cleaned by using a self-cleaning component (25), and the sheath liquid to be used is uniformly stirred by a uniform processing component (9); S3: using an adjustable flow chamber (8) to pump and deliver the cell suspension and sheath fluid, and guiding the cell suspension through a gradually narrowing channel so that the cell group forms a single cell before passing through the laser (22) at the monitoring point; S4: Each cell in the cell suspension moves on the laser beam emitted by the laser (22), and each cell in the sample is analyzed individually in cooperation with the first lens (14) and the first detector (23). When each cell passes through the laser beam, the laser beam will be scattered in multiple directions, and the laser beams scattered in multiple directions are collected and detected by cooperation with each filter (16) and the corresponding second detector (24).

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