Method for improving quality of old melon seeds

Abstract

The invention discloses a method for improving the quality of old muskmelon seeds, and belongs to the technical field of seed treatment.The method comprises the steps that the old muskmelon seeds are subjected to seed priming treatment, then soaked with a resazurin-yeast mixed solution, and finally subjected to secondary priming treatment. By adopting the method disclosed by the invention, the germination potential and germination rate of different varieties of muskmelon old seeds can be simultaneously improved, the germination rate of the muskmelon old seeds can be improved to 85% or above, and the germination potential of the old seeds is close to that of new seeds.

Description

Technical Field

[0001] The invention belongs to the technical field of seed treatment, and in particular relates to a method for improving the quality of old melon seeds. Background Art

[0002] China has a long history of melon cultivation and is the world's largest melon producer and consumer. In the past 10 years, China's melon production has accounted for about 50% of the world's total production. The cultivation of strong seedlings is the primary link to ensure melon production. At present, factory-based seedling cultivation has gradually become the main way to produce melon seedlings, one seed per hole, so seed quality is very important. Although melon seeds can achieve a high germination rate under general production conditions, factors such as unsuitable weather, untimely harvesting, and improper storage will cause the wholesale germination rate of seeds to be lower than 85%, which does not meet national standards. Therefore, it is very meaningful to study methods to improve the germination rate of melons.

[0003] In 1973, Heydecker of the UK used an osmotic agent to treat seeds at a certain temperature and time, and then dried the seeds. He found that the treated seeds could germinate uniformly in a short time at a lower temperature. This seed treatment method (Seed Priming) is also called priming in China. Seed priming is to control the water absorption of seeds so that they stay in the second stage of imbibition, carry out pre-germination physiological and biochemical metabolism and cell membrane and organelle repair, and then dry them before the radicle extends. Although seed priming can promote seed germination and increase the seed germination rate under adverse conditions, it cannot increase the seed germination rate under normal germination conditions (because the moisture, temperature and oxygen under normal germination conditions are sufficient to support the normal germination of seeds).

[0004] Resazurin (also called sodium resazurin) is a chemical substance. The sodium resazurin solution is blue and is widely used in food research to check the content of aerobic bacteria. It changes from blue to pink and finally to colorless in anoxic environments. When bacteria reproduce in milk, resazurin can be reduced to produce color changes. The quality of fresh milk can be judged based on the color changes. The resazurin colorimetric method invented by Tai Gi Min et al. has been applied to the test of the vitality of cruciferous plant seeds. Liu Chunxiang et al. found that the resazurin-yeast mixed reagent can accurately predict the vitality of wheat seeds. This method soaks seeds in a resazurin-yeast solution. Seeds with high vitality have strong cell membrane repair ability and seeds exude substances slowly. Seeds with low vitality exude more and faster substances. Yeast can use the seed exude substances to produce reducing power for respiratory metabolism, so that the resazurin solution changes from blue to pink and finally colorless.

[0005] There is no suitable method in the prior art to simultaneously improve the germination rate and germination potential of old melon seeds. Therefore, a new method for processing old melon seeds has been developed to increase the germination rate of old melon seeds to more than 85%, and the germination potential is close to that of new seeds, which is of great significance. Summary of the invention

[0006] In order to solve the above technical problems, the present invention proposes a method for improving the quality of old melon seeds. The method of the present invention can simultaneously improve the germination potential and germination rate of old melon seeds of different varieties, and can increase the germination rate of old melon seeds to more than 85%, and the germination potential of old seeds is close to that of new seeds.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A method for improving the quality of old melon seeds comprises the following steps: firstly subjecting the old melon seeds to seed priming treatment, then soaking the old melon seeds in a resazurin-yeast mixed solution, and finally subjecting the old melon seeds to a secondary priming treatment.

[0009] Preferably, the seed priming treatment comprises:

[0010] Mix old melon seeds, vermiculite and water to obtain a mixture, and culture the mixture at 15-20° C. for 2-5 days, stirring the mixture once a day during the culture period. After the culture is completed, take out the mixture, separate the old melon seeds, and dry them.

[0011] Preferably, the weight ratio of the aged melon seeds, vermiculite and water is 1:(1-2):(0.6-0.8).

[0012] Preferably, the method of soaking old melon seeds with a resazurin-yeast mixed solution comprises:

[0013] Dissolve resazurin and dry yeast in water to prepare a resazurin-yeast mixed solution (the resazurin-yeast mixed solution must be prepared and used immediately), add the resazurin-yeast mixed solution into a perforated container containing the old melon seeds, put the perforated container into an incubator, culture it in the dark at 28-38° C. for 2-4 hours, then take it out, observe the color of the resazurin-yeast mixed solution in different wells, take out the old melon seeds in the wells where the color of the resazurin-yeast mixed solution is pink or colorless, and finally rinse the old melon seeds in the wells where the color of the resazurin-yeast mixed solution is blue.

[0014] Preferably, the resazurin concentration in the resazurin-yeast mixed solution is 5 to 20 mg / L, and the dry yeast concentration is 5 to 20 g / L.

[0015] Preferably, the perforated container is a homemade container, and the preparation method of the homemade container is: make an organic board of A4 size and 5 mm thickness, and punch 10mm*15mm elliptical holes on the organic board according to the size of melon seeds, and the depth of the elliptical holes is 3mm.

[0016] Preferably, the secondary initiation treatment comprises:

[0017] The washed old melon seeds are mixed with vermiculite, and cultured at 15-20° C. for 2-5 days, with stirring once a day. After the culture, the old melon seeds and vermiculite are separated, and the old melon seeds are dried.

[0018] Preferably, in the secondary priming treatment method, the weight ratio of the rinsed old melon seeds to vermiculite is 1:1.

[0019] Preferably, in the secondary initiation treatment method, the drying treatment is carried out at a temperature of 35° C. and for 48 hours.

[0020] More preferably, the method for improving the quality of old melon seeds is:

[0021] 1) Seed priming: after uniformly mixing old melon seeds, vermiculite and water in a weight ratio of 1:(1-2):(0.6-0.8) to obtain a mixture, the mixture is poured into a container, and then the container is placed in a condition of 15-20° C. for culturing for 2-5 days, during which the mixture is stirred once a day, and after the culturing is completed, the mixture is taken out, the old melon seeds and the vermiculite are separated by a sieve, and then the old melon seeds are placed in a forced air drying oven for drying for 48 hours at a drying temperature of 35° C.;

[0022] 2) Seed board preparation: Prepare an A4 size, 5 mm thick organic board, punch a 10 mm*15 mm elliptical hole on the board according to the size of the old melon seeds, the depth of the elliptical hole is 3 mm, and then place the primed old melon seeds into the elliptical hole;

[0023] 3) Soaking in a resazurin-yeast mixed solution: Weigh resazurin and dry yeast and dissolve them in pure water to prepare a resazurin-yeast mixed solution, wherein the resazurin concentration is 5-20 mg / L and the dry yeast concentration is 5-20 g / L; then use a dropper to draw 0.5-1.5 mL of the prepared resazurin-yeast mixed solution and drip it into the oval hole containing the old melon seeds. After all the resazurin-yeast mixed solution is dripped into the organic plate, the plate is placed in an incubator at 28-38° C., cultured in the dark for 2-4 hours, and then taken out, and the color of the resazurin-yeast mixed solution in different oval holes is observed. The old melon seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless are taken out, and finally the old melon seeds whose resazurin-yeast mixed solution in the oval hole is blue are all poured into a gauze bag and rinsed with clean water;

[0024] 4) Secondary initiation: the old melon seeds rinsed with water in the previous step and vermiculite are mixed evenly in a weight ratio of 1:1 and poured into a container, and then the container is placed in a 15-20°C condition for 2-5 days, stirred once a day, and after the cultivation is completed, the old melon seeds and vermiculite are separated by a sieve, and then the old melon seeds are placed in a blast drying oven for 48 hours, and the drying temperature is 35°C. The obtained old melon seeds are high-quality old melon seeds. The high-quality old melon seeds of the present invention have the advantages of high germination potential and germination rate, and the high-quality old melon seeds can be dried and sealed for storage.

[0025] Although the vigor of some seeds (such as wheat seeds and common cabbage seeds) can be predicted by using a resazurin-yeast mixed solution, the present invention directly uses a resazurin-yeast mixed solution to soak muskmelon seeds, and it is found that the color change reaction of the resazurin-yeast mixed solution has no significant correlation with the vigor of muskmelon seeds, and cannot be used to accurately predict the vigor of muskmelon seeds. In the method for improving the quality of muskmelon seeds of the present invention, the repair of the cell membrane of vigorous muskmelon seeds is promoted after seed priming, and the vigor is enhanced. In terms of the speed of exudation of substances after absorbing water, the gap between vigorous and inviolable is increased. Therefore, in the subsequent resazurin-yeast color development reaction, the vigorous muskmelon seeds are not easy to change color within a suitable time, while the inviolable muskmelon seeds change color quickly. The muskmelon seeds with pink or colorless solutions are removed by color difference, and the muskmelon seeds with blue solutions are still vigorous and have a high germination rate. Then, through the secondary priming process, the germination potential of the batch of muskmelon seeds can be improved, so as to achieve the purpose of improving the germination potential and germination rate at the same time.

[0026] Compared with the prior art, the present invention has the following advantages and technical effects:

[0027] Without initiation, there is no obvious difference between the vital and inanimate old melon seeds in the speed of exuding substances after absorbing water, which is manifested in that the vital old melon seeds are also easy to change color in the resazurin-yeast color development test. The present invention skillfully combines the initiation technology and resazurin-yeast, and can identify the inanimate old melon seeds in the batch of old melon seeds, and improve the germination rate of the batch of old melon seeds by removing the inanimate old melon seeds. If the old melon seeds are directly washed and dried after the color development reaction, the germination potential of the old melon seeds will be reduced. The present invention creatively introduces a secondary initiation process, which can further improve the germination potential of the old melon seeds, thereby achieving the purpose of simultaneously improving the germination potential and germination rate of the old melon seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0029] Figure 1 This is the seed plate effect diagram after the old Hami red melon seeds were soaked in the resazurin-yeast mixed solution for 4 hours in step 3) of Example 1;

[0030] Figure 2 This is the seed plate effect diagram after the old Hami red melon seeds were soaked in the resazurin-yeast mixed solution for 1 hour in step 3 of Example 1;

[0031] Figure 3 The germination experiment diagram of the old Hami red melon seeds (left) whose solution is blue and the old Hami red melon seeds (right) which are pink or colorless in step 3) of Example 1;

[0032] Figure 4 The germination results of the old Hami red melon seeds (left) whose solution appears blue and the old Hami red melon seeds (right) which are pink or colorless in step 3) of Example 1 are shown. DETAILED DESCRIPTION

[0033] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0034] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0035] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0036] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0037] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0038] In the present invention, the term "old seeds" refers to seeds that have been stored for a long time (usually more than one year) and whose physiological activity, germination rate and quality condition have relatively declined; "new seeds" refers to seeds produced within the last year and have been properly processed, dried and stored.

[0039] All raw materials and reagents used in the examples of the present invention were purchased from commercial sources. As an example, resazurin (S19084) and dry yeast (yeast II, Y41643) were purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0040] The technical solution of the present invention is further illustrated by the following embodiments.

[0041] Example 1

[0042] 1) Seed priming: Weigh 500 grams of Hami red melon old seeds (germination rate of about 50%) and 500 grams of vermiculite with a particle size of about 2 mm into a plastic container, then add 400 milliliters of water and mix thoroughly to obtain a mixture. Then place the container at 20°C for 3 days, stirring the mixture once a day. After the cultivation is completed, take out the mixture, separate the Hami red melon old seeds and vermiculite with a sieve, and then put the Hami red melon old seeds into a forced air drying oven and dry them for 48 hours at a drying temperature of 35°C.

[0043] 2) Seed board preparation: Make an A4 size, 5 mm thick organic board, punch a 10 mm*15 mm elliptical hole on the board according to the size of Hami red melon seeds, the depth of the elliptical hole is 3 mm, and then put the primed Hami red melon seeds into the elliptical hole.

[0044] 3) Soaking in resazurin-yeast mixed solution: Weigh 15 mg of resazurin and 20 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 0.5 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole where the Hami red melon old seeds are placed. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in a 30°C incubator. After culturing in the dark for 3 hours, take it out and observe the color of the resazurin-yeast mixed solution in different holes. Take out the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless. Finally, pour all the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is blue into a gauze bag and rinse it with clean water.

[0045] 4) Secondary initiation: The old Hami red melon seeds rinsed with water in the previous step and vermiculite are poured into a container at a weight ratio of 1:1 and mixed evenly, and then the container is placed at 15°C for 4 days, and stirred once a day. After the incubation, the old Hami red melon seeds and vermiculite are separated by a sieve, and then the old Hami red melon seeds are placed in a forced air drying oven for 48 hours at a drying temperature of 35°C. After drying, the treated old Hami red melon seeds are obtained and sealed and stored for use.

[0046] In this embodiment, step 3) the old Hami red melon seeds are soaked in a resazurin-yeast mixed solution for 4 hours. The seed plate effect diagram is shown in FIG. Figure 1 ; In this embodiment, step 3) the old Hami red melon seeds were soaked in a resazurin-yeast mixed solution for 1 hour. The seed plate effect diagram is shown in FIG. Figure 2 .

[0047] In this embodiment, the germination experiment of Hami red melon old seeds (left) with blue solution and pink or colorless Hami red melon old seeds (right) is shown in the seed placement diagram. Figure 3 In this embodiment, the germination results of the Hami red melon old seeds (left) with blue solution and the Hami red melon old seeds (right) with pink or colorless solution are shown in FIG. Figure 4 It can be seen that the present invention skillfully combines the priming technology and resazurin-yeast, and can identify the lifeless old melon seeds in the old melon seed batch, and improve the germination rate of the old melon seed batch by removing the lifeless old melon seeds.

[0048] Comparative Example 1

[0049] Aged Hami red melon seeds that have not undergone any processing.

[0050] Comparative Example 2 (directly soaking old melon seeds with a resazurin-yeast mixed solution without any priming treatment)

[0051] 1) Seed board preparation: Make an A4 size, 5 mm thick organic board, punch a 10 mm*15 mm elliptical hole on the board according to the size of Hami red melon old seeds, the depth of the elliptical hole is 3 mm, and then put the primed Hami red melon old seeds into the elliptical hole.

[0052] 2) Soaking in a resazurin-yeast mixed solution: Weigh 15 mg of resazurin and 20 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 0.5 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole where the Hami red melon old seeds are placed. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in an incubator at 30°C, and incubated in the dark for 3 hours before being taken out. The color of the resazurin-yeast mixed solution in different holes is observed, and the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless are taken out. Finally, the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is blue are all poured into a gauze bag, rinsed with clean water, and dried to obtain the treated Hami red melon old seeds.

[0053] Comparative Example 3 (without secondary initiation treatment)

[0054] 1) Seed priming: Weigh 500 grams of Hami red melon old seeds (germination rate of about 50%) and 500 grams of vermiculite with a particle size of about 2 mm into a plastic container, then add 400 milliliters of water and mix thoroughly to obtain a mixture. Then place the container at 20°C for 3 days, stirring the mixture once a day. After the incubation, take out the mixture and use a sieve to separate the Hami red melon old seeds and vermiculite, then put the Hami red melon old seeds into a forced air drying oven and dry them for 48 hours at a drying temperature of 35°C.

[0055] 2) Seed board preparation: Make an A4 size, 5 mm thick organic board, punch a 10 mm*15 mm elliptical hole on the board according to the size of Hami red melon seeds, the depth of the elliptical hole is 3 mm, and then put the primed Hami red melon seeds into the elliptical hole.

[0056] 3) Soaking in a resazurin-yeast mixed solution: Weigh 15 mg of resazurin and 20 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 0.5 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole where the Hami red melon old seeds are placed. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in an incubator at 30°C, and incubated in the dark for 3 hours before being taken out. The color of the resazurin-yeast mixed solution in different holes is observed, and the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless are taken out. Finally, the Hami red melon old seeds whose resazurin-yeast mixed solution in the oval hole is blue are all poured into a gauze bag, rinsed with clean water, and dried to obtain the treated Hami red melon old seeds.

[0057] Comparative Example 4

[0058] New Hami red melon seeds that have not undergone any treatment.

[0059] Example 2

[0060] 1) Seed priming: Weigh 100 grams of Qibao Golden Melon old seeds (germination rate of about 60%) and 200 grams of vermiculite with a particle size of about 0.5 mm into a plastic container, then add 80 milliliters of water and mix thoroughly to obtain a mixture. Then place the container at 15°C for 5 days, stirring the mixture once a day. After the cultivation is completed, take out the mixture and use a sieve to separate the seeds and vermiculite, then put the seeds into a forced air drying oven and dry them for 48 hours at a drying temperature of 35°C.

[0061] 2) Seed board preparation: Make an A4 size, 5mm thick organic board, punch a 10mm*15mm elliptical hole on the board according to the size of Qibao Golden Melon Muskmelon seeds, the depth of the elliptical hole is 3mm, and then put the primed Qibao Golden Melon Muskmelon seeds into the elliptical hole.

[0062] 3) Soaking in resazurin-yeast mixed solution: Weigh 5 mg of resazurin and 10 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 0.5 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole where the Qibao Golden Melon muskmelon old seeds are placed. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in a 35°C incubator. After 3.5 hours of incubation under dark conditions, take it out and observe the color of the resazurin-yeast mixed solution in different oval holes. Take out the Qibao Golden Melon muskmelon old seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless. Finally, pour all the Qibao Golden Melon muskmelon old seeds whose resazurin-yeast mixed solution in the oval hole is blue into a gauze bag and rinse it with clean water.

[0063] 4) Secondary initiation: Pour the Qibao Golden Melon Muskmelon old seeds rinsed with water in the previous step and the vermiculite into a container at a weight ratio of 1:1 and mix them evenly, then place the container at 20°C for 2 days, stirring once a day. After the incubation, use a sieve to separate the Qibao Golden Melon Muskmelon old seeds and vermiculite, then put the Qibao Golden Melon Muskmelon old seeds into a forced air drying oven for 48 hours at a drying temperature of 35°C, and obtain the processed Qibao Golden Melon Muskmelon old seeds after drying, which are sealed and stored for later use.

[0064] Comparative Example 5

[0065] Qibao golden melon old seeds that have not been processed in any way.

[0066] Comparative Example 6

[0067] 1) Seed board preparation: Make an A4 size, 5mm thick organic board, punch a 10mm*15mm elliptical hole on the board according to the size of Qibao Golden Melon Muskmelon seeds, the depth of the elliptical hole is 3mm, and then put the primed Qibao Golden Melon Muskmelon seeds into the elliptical hole.

[0068] 2) Soaking in a resazurin-yeast mixed solution: Weigh 5 mg of resazurin and 10 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 0.5 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole where the Qibao Golden Melon Muskmelon Old Seeds are placed. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in a 35°C incubator. After 3.5 hours of incubation under dark conditions, it is taken out and the color of the resazurin-yeast mixed solution in different oval holes is observed. The Qibao Golden Melon Muskmelon Old Seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless are taken out. Finally, the Qibao Golden Melon Muskmelon Old Seeds whose resazurin-yeast mixed solution in the oval hole is blue are all poured into a gauze bag, rinsed with clean water, and dried to obtain the treated Qibao Golden Melon Muskmelon Old Seeds.

[0069] Comparative Example 7

[0070] The same as Example 2, except that no secondary priming treatment is performed, and the Qibao golden melon muskmelon old seeds rinsed with clean water in step 3) are dried to obtain the treated Qibao golden melon muskmelon old seeds.

[0071] Comparative Example 8

[0072] New Qibao golden melon seeds that have not undergone any processing.

[0073] Example 3

[0074] 1) Seed priming: Weigh 1000 g of old red superior melon seeds (germination rate of about 70%) and 1000 g of vermiculite with a particle size of about 1 mm into a plastic container, then add 700 ml of water and mix thoroughly to obtain a mixture. Then place the container at 20°C for 3 days, stirring the mixture once a day. After the incubation, take out the mixture and use a sieve to separate the old red superior melon seeds and vermiculite, then put the old red superior melon seeds into a forced air drying oven and dry them for 48 hours at a drying temperature of 35°C.

[0075] 2) Preparation of Red Superior Melon Seed Board: Make an A4 size, 5mm thick organic board, punch a 10mm*15mm elliptical hole on the board according to the shape of the Red Superior Melon Seed. The depth of the elliptical hole is 3mm, and then put the primed Red Superior Melon Seed into the elliptical hole.

[0076] 3) Soaking in a resazurin-yeast mixed solution: Weigh 10 mg of resazurin and 20 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 1 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole containing the old red superior melon seeds. After all the resazurin-yeast mixed solution has been dropped, the organic plate is placed in an incubator at 30°C. After incubation in the dark for 2.5 hours, take it out and observe the color of the resazurin-yeast mixed solution in different oval holes. Take out the old red superior melon seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless. Finally, pour all the old red superior melon seeds whose resazurin-yeast mixed solution in the oval hole is blue into a gauze bag and rinse with clean water.

[0077] 4) Secondary initiation: The old red melon seeds rinsed with water in the previous step and vermiculite are poured into a container at a weight ratio of 1:1 and mixed evenly, and then the container is placed at 15°C for 5 days, and stirred once a day. After the incubation, the old red melon seeds and vermiculite are separated by a sieve, and then the old red melon seeds are placed in a forced air drying oven for 48 hours at a drying temperature of 35°C. After drying, the treated old red melon seeds are obtained and sealed and stored for later use.

[0078] Comparative Example 9

[0079] Old red melon seeds that have not been processed in any way.

[0080] Comparative Example 10

[0081] 1) Preparation of Red Superior Melon Seed Board: Make an A4 size, 5mm thick organic board, punch a 10mm*15mm elliptical hole on the board according to the shape of the Red Superior Melon Seed. The depth of the elliptical hole is 3mm, and then put the primed Red Superior Melon Seed into the elliptical hole.

[0082] 2) Soaking in a resazurin-yeast mixed solution: Weigh 10 mg of resazurin and 20 g of dry yeast and dissolve them in 1 liter of pure water to prepare a resazurin-yeast mixed solution. Then use a dropper to draw 1 ml of the prepared resazurin-yeast mixed solution and drop it into the oval hole containing the red superior melon old seeds. After all the resazurin-yeast mixed solution is dropped, the organic plate is placed in an incubator at 30°C, and cultured in the dark for 2.5 hours. After that, take it out and observe the color of the resazurin-yeast mixed solution in different oval holes. Take out the red superior melon old seeds whose resazurin-yeast mixed solution in the oval hole is pink or colorless. Finally, pour all the red superior melon old seeds whose resazurin-yeast mixed solution in the oval hole is blue into a gauze bag, rinse it with clean water, and dry it to obtain the treated red superior melon old seeds.

[0083] Comparative Example 11

[0084] The same as Example 3, except that no secondary priming treatment is performed, and the old red superior melon seeds rinsed with clean water in step 3) are dried to obtain the treated old red superior melon seeds.

[0085] Comparative Example 12

[0086] New seeds of red melon that have not undergone any treatment.

[0087] The melon seeds finally obtained in the above embodiments and comparative examples were subjected to an indoor germination experiment. The germination method referred to the germination technical method in the national seed inspection regulations ("Germination Test in the Inspection Regulations for Crop Seeds" (GB / T 3543.4-1995)). Vermiculite was used as the germination medium. The germination was carried out at 28°C. 100 seeds were treated for four times. The germination was calculated based on the breaking of the cotyledons. The germination potential was counted for 3 days and the germination rate was counted for 8 days. The results are shown in Table 1.

[0088] Table 1

[0089]

[0090] As shown in Table 1, the germination potential and germination rate of the old seeds of melon varieties with a germination rate lower than 85% obtained by the treatment method of the present invention are improved, among which the germination rate of the old melon seeds is improved to more than 85%, and the germination potential of the old seeds is close to that of the new seeds, indicating that the method of the present invention can simultaneously improve the germination potential and germination rate of the old seeds of different varieties of melon. The germination rate and germination potential of the old seeds obtained by soaking only in the resazurin-yeast solution are close to those of the old seeds without any treatment, indicating that the germination rate cannot be improved by soaking only in the resazurin-yeast solution. After the first three steps of the method of the present invention, there is no secondary priming treatment. Although the germination potential and germination rate of the seeds are improved compared with the old seeds without any treatment, the germination rate and germination potential of the seeds are lower than the germination rate and germination potential of the seeds after the seed priming treatment and the secondary priming treatment, indicating that the germination potential and germination rate of the old melon seeds obtained by the four steps of the present invention are the highest, close to the germination potential and germination rate of the new seeds.

[0091] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A method for improving the quality of old melon seeds, characterized in that: Firstly, the old melon seeds are subjected to seed priming treatment, then the old melon seeds are soaked in a resazurin-yeast mixed solution, and finally the old melon seeds are subjected to secondary priming treatment.

2. The method for improving the quality of old melon seeds according to claim 1, characterized in that: The seed priming treatment comprises: Mix old melon seeds, vermiculite and water to obtain a mixture, and culture the mixture at 15-20° C. for 2-5 days, stirring the mixture once a day during the culture period. After the culture is completed, take out the mixture, separate the old melon seeds, and dry them.

3. The method for improving the quality of old melon seeds according to claim 2, characterized in that: The weight ratio of the aged melon seeds, vermiculite and water is 1:(1-2):(0.6-0.8).

4. The method for improving the quality of old melon seeds according to claim 1, characterized in that: The method of soaking old melon seeds with a resazurin-yeast mixed solution comprises: Dissolve resazurin and dry yeast in water to prepare a resazurin-yeast mixed solution, add the resazurin-yeast mixed solution into a perforated container containing the old melon seeds, put the perforated container into an incubator, culture it in the dark at 28-38° C. for 2-4 hours, then take it out, observe the color of the resazurin-yeast mixed solution in different wells, take out the old melon seeds in the wells where the color of the resazurin-yeast mixed solution is pink or colorless, and finally rinse the old melon seeds in the wells where the color of the resazurin-yeast mixed solution is blue.

5. The method for improving the quality of old melon seeds according to claim 4, characterized in that: The resazurin concentration in the resazurin-yeast mixed solution is 5-20 mg / L, and the dry yeast concentration is 5-20 g / L.

6. The method for improving the quality of old melon seeds according to claim 1, characterized in that: The secondary initiation treatment comprises: The washed old melon seeds are mixed with vermiculite, and cultured at 15-20° C. for 2-5 days, with stirring once a day. After the culture, the old melon seeds and vermiculite are separated, and the old melon seeds are dried.

7. The method for improving the quality of old melon seeds according to claim 6, characterized in that: The weight ratio of the washed old melon seeds to the vermiculite is 1:

1.

8. The method for improving the quality of old melon seeds according to claim 6, characterized in that: The drying temperature is 35°C and the drying time is 48 hours.

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