Composition for promoting plant growth and use thereof

Abstract

The invention provides a composition for promoting plant growth and application thereof, the composition comprises cyclic lipopeptide and linear lipopeptide, and the composition can give full play to the advantages of the cyclic lipopeptide and the linear lipopeptide by combining the cyclic lipopeptide and the linear lipopeptide, promote absorption and utilization of nutrients by plants, promote growth and development of crops, improve yield and quality, and improve the yield and the quality of plants. The requirements of people on high-quality agricultural products are met, huge social, ecological and economic benefits are created, and the method is worthy of vigorous popularization.

Description

Technical Field

[0001] The present application relates to the fields of biotechnology and biochemical engineering, and in particular to a composition for promoting plant growth and its use. Background Art

[0002] Lipopeptides are usually a class of biosurfactants composed of amino or hydroxy fatty acids and peptide chains or peptide rings. They have a wide range of biological activities and are generally synthesized by microorganisms such as Bacillus subtilis, Bacillus polymyxa, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus atrophaeus, Bacillus methylotrophicus, Bacillus paralicheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, etc.

[0003] According to the structural characteristics of lipopeptides, they are divided into cyclic lipopeptides and linear lipopeptides. Cyclic lipopeptides refer to a type of lipopeptides with a cyclic structure. The carboxyl group of the amino acid at the C-terminal end of the peptide chain is connected to the amino group or hydroxyl group in the peptide or fatty acid to form a cyclic structure. The components that form the cyclic structure are mainly amino acids, and the fatty acid (hydroxy fatty acid or amino fatty acid) chain in some lipopeptides also participates in the ring formation. Linear lipopeptides are a type of lipopeptides in which amino acids are connected in sequence into a linear shape, are not connected head to tail, and have no cyclic structure. Fatty acids are connected to the a-amino group or other hydroxyl groups at the N-terminal end of the peptide chain, and have both straight-chain structures and branched-chain structures. Because lipopeptides have good surface activity, emulsification, and foaming abilities, they have broad application prospects in the fields of oil extraction, biomedicine, agriculture, and daily chemicals. In agriculture, lipopeptides also have significant effects on promoting nutrient absorption, crop growth, and improving crop quality, but how to compound different types of lipopeptides has not been studied. Summary of the invention

[0004] In the agricultural field, the application of lipopeptides is still in a relatively weak stage, and the combined use of cyclic lipopeptides and linear lipopeptides is even blank.

[0005] In order to solve the problems existing in the practical application of lipopeptides in the prior art, the present application provides a composition for promoting plant growth and its use, wherein the composition can significantly promote plant growth and improve plant quality.

[0006] The specific technical solutions of this application are as follows:

[0007] 1. A composition for promoting plant growth, comprising a cyclic lipopeptide and a linear lipopeptide.

[0008] 2. The composition according to item 1, wherein the cyclic lipopeptide comprises one or more selected from the group consisting of surfactin, lichenin, iturin family, echinocandins, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or

[0009] The linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

[0010] 3. The composition according to item 1 or 2, wherein the cyclic lipopeptide is 0.01-40%, preferably 0.01-20%; the linear lipopeptide is 0.01-20%, preferably 1-10% by mass in the composition;

[0011] Preferably, the mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500.

[0012] 4. A composition according to any one of items 1 to 3, wherein the composition further comprises an active ingredient and / or an agriculturally acceptable adjuvant;

[0013] Preferably, the dosage form of the composition comprises one selected from aqueous solution, suspension, granule, powder, seed coating, suspoemulsion and soluble liquid.

[0014] 5. Use of the composition described in any one of items 1 to 4 in the preparation of biological bacterial agents, bacterial fertilizers, amino acid-containing water-soluble fertilizers or organic water-soluble fertilizers.

[0015] 6. Use of linear lipopeptides in promoting plant growth.

[0016] 7. The use according to item 6, wherein the linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

[0017] 8. Use of linear lipopeptides in enhancing the effect of cyclic lipopeptides in promoting plant growth.

[0018] 9. The use according to item 8, wherein the cyclic lipopeptide comprises one or more selected from surfactin, lichenin, iturin family, echinocandins, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or

[0019] The linear lipopeptide is obtained by fermentation using Brevibacillus laterus;

[0020] The mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500;

[0021] Preferably, the concentration of the cyclic lipopeptide is 0.01-40wt%, preferably 0.01-20wt%;

[0022] The linear lipopeptide is 0.01-20wt%, preferably 1-10wt%.

[0023] 10. A method for promoting plant growth, comprising applying the composition described in any one of items 1 to 4 to a plant, thereby promoting the growth of the plant.

[0024] Effects of the Invention

[0025] The composition described in the present application contains cyclic lipopeptides and linear lipopeptides, which can significantly promote plant growth and improve plant quality.

[0026] The composition described in the present application can give full play to the advantages of two lipopeptide compositions, and will be of great potential in increasing crop yields. In addition, the promotion and application of lipopeptide biological preparations can effectively promote the development of green and sustainable ecological agriculture.

[0027] The composition described in the present application uses linear lipopeptides and cyclic lipopeptides, which can promote the absorption and utilization of nutrients by plants, promote the growth and development of crops, increase yield and quality, meet people's demand for high-quality agricultural products, provide more choices for the market, create huge social, ecological and economic benefits, and deserve to be vigorously promoted. DETAILED DESCRIPTION

[0028] The present application is described in detail below in conjunction with the described embodiments. Although specific embodiments of the present application are shown, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to enable the scope of the present application to be fully communicated to those skilled in the art.

[0029] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the functional differences of components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" are open-ended terms and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present application, but the description is based on the general principles of the specification and is not intended to limit the scope of the present application. The scope of protection of the present application shall be determined by the attached claims.

[0030] The present application provides a composition for promoting plant growth, comprising a cyclic lipopeptide and a linear lipopeptide. In some embodiments, the cyclic lipopeptide comprises one or more selected from surfactin, lichenin, iturin family, echinocandin, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or

[0031] The linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

[0032] In the present application, surfactin, lichenin, iturin family, echinocandin, fengycin, phosphatidylinositol, octapeptidemycin, polymyxin, and fusaricide can be purchased commercially or prepared by conventional methods in the art.

[0033] In the present application, there is no limitation on the method of obtaining linear lipopeptides by fermentation using Brevibacillus laterus, and the fermentation can be carried out by conventional methods in the art;

[0034] For example, Brevibacillus laterus is subjected to slant strain activation, seed shake flask culture, seed tank culture and fermentation culture to obtain the product.

[0035] In the present application, for activation of slant strains, for example, Brevibacillus laterosporus refrigerated tube strains can be inoculated into LB slant medium for culture.

[0036] For seed shake flask culture, the slant culture is inoculated into a sterilized seed culture medium and shaken to obtain a seed solution. In this step, the present application does not impose any restrictions on the formula of the seed culture medium, and the seed culture medium conventional in the art can be used. For example, in some embodiments, the formula of the seed culture medium can be: yeast extract 5-20g, corn syrup powder 5-20g, glucose 20-30g, sodium chloride 3-10g, potassium dihydrogen phosphate 0.1-1g, calcium carbonate 2-5g, water 1000ml, pH 6.0-7.0.

[0037] In the present application, the conditions for shake flask culture are not limited in any way, and those skilled in the art can make routine selections as needed, for example, the conditions for shake flask seed culture are 32-38°C, and / or

[0038] The speed is 200 rpm; and / or

[0039] Shake flask culture for 8-16 hours to obtain seed solution; and / or

[0040] The concentration of Brevibacterium cells was 10 7 -10 8 cfu / ml.

[0041] For seed tank culture, for example, the seed culture medium in the primary seed tank can be sterilized, and then inoculated with the seed liquid obtained by seed shake flask culture to obtain the seed liquid.

[0042] In the present application, the culture conditions in the seed tank are not limited in any way, and those skilled in the art can make routine selections as needed. For example, the culture conditions in the seed tank can be:

[0043] Seed tank culture conditions are 0.2-5% inoculum; and / or

[0044] The culture temperature is 32-38°C; and / or

[0045] The stirring speed is 40-50HZ; and / or

[0046] Ventilation rate of 1:0.8-1.2; and / or

[0047] Tank pressure is 0.04-0.05MPa; and / or

[0048] The culture time is 8-16 hours to obtain seed solution; and / or

[0049] The bacterial concentration was 10 7 -10 8 cfu / ml.

[0050] For fermentation culture, for example, the obtained seed liquid can be inoculated into the fermentation medium for fermentation. During the fermentation culture process, for example, the dissolved oxygen can be controlled at more than 20%; and during the fermentation period of 10-20 hours, 0.2-2g of FP101 tryptone is added per liter of fermentation liquid, the pH is 6.0-6.5, and the foam is controlled during the high foam period. The total fermentation culture period is 12-25hr.

[0051] In the present application, there is no limitation on the formula of the fermentation medium, and those skilled in the art can make routine selections as needed. For example, the fermentation medium formula is 20-50 g glucose, 5-20 g glycerol, 2-5 g inorganic ammonium salt, 0.05-0.5 g dipotassium hydrogen phosphate, 3-10 g yeast powder or corn syrup powder, 5-15 g industrial peptone, 5-10 g sodium chloride, 1-3 g calcium chloride, 1-4 g Val, 1000 ml water, PH 6.0-7.0.

[0052] Defoaming agents can be used to defoam during the peak foaming period, such as the composite defoaming agent T31.

[0053] In the present application, the Brevibacillus laterosporus may be Brevibacillus laterosporus CGMCC No.16337.

[0054] In the present application, the linear lipopeptide can be prepared by the method disclosed in Chinese patent application CN112553276A, and confirmed using the method disclosed by TODD BARSBY in new antibiotics from a marine isolate of BACILLUSLATEROSPORUS (frontiers in micirobiology, published on October 29, 2021, doi:10.3389 / fmicb.2021.729026).

[0055] The present application can promote plant growth by combining cyclic lipopeptides and linear lipopeptides. The addition of linear lipopeptides can reduce the usage of cyclic lipopeptides and achieve similar or improved technical effects.

[0056] In some embodiments, the cyclic lipopeptide is 0.01-40%, preferably 0.01-20%, by mass percentage in the composition;

[0057] The linear lipopeptide is 0.01-20%, preferably 1-10%.

[0058] For example, in terms of mass percentage in the composition, the cyclic lipopeptide is 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 25%, 30%, 35%, 40%, etc.;

[0059] The linear lipopeptide is 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, etc.

[0060] In some embodiments, the mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500.

[0061] For example, the mass ratio (m 环状脂肽 :m 线性脂肽 ) can be 1:0.5, 1:1, 1:5, 1:10, 1:20, 1:50, 1:75, 1:100, 1:150, 1:200, 1:250, 1:300, 1:350, 1:400, 1:450, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, etc.

[0062] In some embodiments, the composition further comprises an active ingredient and / or an agriculturally acceptable adjuvant.

[0063] In the present application, the active ingredients and agriculturally acceptable adjuvants are the active ingredients and adjuvants commonly used in the art, and the present application does not impose any limitation thereto.

[0064] In some embodiments, the dosage form of the composition comprises one selected from aqueous solution, suspension, granule, powder, seed coating, suspoemulsion and soluble liquid.

[0065] In the present application, suitable adjuvants are added to the composition to prepare an aqueous solution, a suspension, a granule, a powder, a seed coating agent, a suspoemulsion or a soluble liquid, etc.

[0066] In the present application, there is no limitation on suitable auxiliary agents, and those skilled in the art can choose to add suitable auxiliary agents based on the needs of the dosage form to obtain the above-mentioned dosage form.

[0067] The present application provides the use of the above-mentioned composition in the preparation of biological bacterial agents, bacterial fertilizers, water-soluble fertilizers containing amino acids or organic water-soluble fertilizers.

[0068] The composition described in the present application can be used to prepare biological bacterial agents, bacterial fertilizers, water-soluble fertilizers containing amino acids or organic water-soluble fertilizers, thereby acting on plants to promote plant growth.

[0069] The present application provides the use of linear lipopeptides in promoting plant growth. In some embodiments, the linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

[0070] The inventors of the present application discovered during the study of lipopeptides that linear lipopeptides can promote plant growth and increase plant height, stem thickness and yield.

[0071] The present application provides the use of linear lipopeptides in improving the growth promotion of cyclic lipopeptides in plants. In some embodiments, the cyclic lipopeptide comprises one or more selected from surfactin, lichenin, iturin family, echinocandin, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or

[0072] The linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

[0073] The present application adds linear lipopeptides to cyclic lipopeptides and acts on plants, which can greatly improve the growth of plants, for example, it can increase the plant height, stem thickness, yield, etc.

[0074] In some embodiments, the mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500.

[0075] For example, the mass ratio (m 环状脂肽 :m 线性脂肽 ) can be 1:0.5, 1:1, 1:5, 1:10, 1:20, 1:50, 1:75, 1:100, 1:150, 1:200, 1:250, 1:300, 1:350, 1:400, 1:450, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, etc.

[0076] In some embodiments, the concentration of the cyclic lipopeptide is 0.01-40 wt %, preferably 0.01-20 wt %;

[0077] The linear lipopeptide is 0.01-20wt%, preferably 1-10wt%.

[0078] For example, the cyclic lipopeptide is 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 2wt%, 3wt%, 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%, 11wt%, 12wt%, 13wt%, 14wt%, 15wt%, 16wt%, 17wt%, 18wt%, 19wt%, 20wt%, 25wt%, 30wt%, 35wt%, 40wt%, etc.;

[0079] The linear lipopeptide is 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 2wt%, 3wt%, 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%, 11wt%, 12wt%, 13wt%, 14wt%, 15wt%, 16wt%, 17wt%, 18wt%, 19wt%, 20wt% and the like.

[0080] The present application provides a method for promoting plant growth, which comprises applying any of the above-mentioned compositions to plants, thereby promoting the growth of the plants.

[0081] In the present application, there is no limitation on the mode of action, and the composition can be applied to the composition in a conventional manner in the art, such as by root irrigation or foliar spraying.

[0082] In the present application, regarding the method of using the composition, the composition can be diluted first and then acted on the plant. Preferably, the composition is diluted 100-1000 times, preferably 500 times, and then acted on the plant.

[0083] For example, the composition can be diluted 100 times, 200 times, 300 times, 400 times, 500 times, 600 times, 700 times, 800 times, 900 times, 1000 times, etc.

[0084] In the present application, the plant may be, for example, a field crop or an economic crop, for example, the plant may be rice, wheat, soybean, corn, tomato, cucumber, pepper, strawberry, etc.

[0085] Example

[0086] The present application provides a general and / or specific description of the materials and test methods used in the experiment. In the following examples, if there is no other special description, % means wt%, that is, weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be purchased commercially, among which Fengyuansu is purchased from MedChemExpress; iturin is purchased from MedChemExpress; lichenin is purchased from Aladdin; fusaricide is purchased from Maclean; echinocandin is purchased from Chemical Book; octapeptidemycin is purchased from MedChemExpress; polymyxin is purchased from Maclean; phospholipids are purchased from Chemical Book; linear lipopeptides are prepared by the method disclosed in Chinese patent application CN112553276A to obtain fermentation broth, and the method disclosed by TODD BARSBY in new antibiotics from a marine isolate of BACILLUS LATEROSPORUS is used to confirm that it is a fermentation broth containing linear lipopeptides, and the linear lipopeptides are purified by conventional methods in the art.

[0087] Surfactin A fermentation broth containing surfactin is prepared according to the method disclosed in Chinese patent application CN109097315A, and the surfactin is purified by a conventional method in the art.

[0088] Example 1

[0089] The surfactin and the linear lipopeptide were mixed with water to obtain compositions 12-15, 17-20 and 35-39, wherein the contents of the surfactin and the linear lipopeptide and the water are as shown in Table 1;

[0090] Alternatively, the linear lipopeptide is mixed with water to obtain compositions 11 and 16, wherein the contents of the linear lipopeptide and water are respectively as shown in Table 1.

[0091] Example 2

[0092] The fengyuan element and the linear lipopeptide were mixed with water to obtain compositions 21-24, wherein the contents of the fengyuan element, the linear lipopeptide and the water were as shown in Table 1.

[0093] Example 3

[0094] Iturin and linear lipopeptide were mixed with water to obtain compositions 25-28, wherein the contents of iturin, linear lipopeptide and water were as shown in Table 1.

[0095] Example 4

[0096] Lichenin, fusaricide, echinocandin, octapeptide, polymyxin and phosphatidylserine are mixed with linear lipopeptide and water respectively to obtain compositions 29-34, wherein the contents of lichenin, fusaricide, echinocandin, octapeptide, polymyxin, phosphatidylserine and linear lipopeptide and water are as shown in Table 1.

[0097] Comparative Example 1

[0098] The surfactin was mixed with water to obtain composition 2-10, wherein the contents of the surfactin and water were as shown in Table 1.

[0099] Comparative Example 2

[0100] Composition 1 contained only water.

[0101] Table 1

[0102]

[0103]

[0104] Experimental Example 1 First, the effect of simple cyclic lipopeptides on cucumber was studied.

[0105] (1) Test crops: cucumber, cultivar Zhongnong 16. Cucumber plants with uniform growth were selected and planted in pots with a radius of 10 cm.

[0106] (2) Test agents: Compositions 1-10.

[0107] (3) Experimental design: The experimental treatment settings are as follows:

[0108] Compositions 1-10 were diluted 500 times and applied to the roots, and each treatment was repeated 3 times, applied once every other week, and applied 3 times in total. 10 days after the third application, the plant height, stem diameter and chlorophyll growth index of each cucumber sample were measured, and the results are shown in Table 2, wherein the chlorophyll content was measured using a conventional chlorophyll meter in the art (YT-YD plant nutrition meter, purchased from Shandong Yuntang Intelligent Technology Co., Ltd.).

[0109] Table 2

[0110] deal with Plant height(cm) Stem diameter (mm) Chlorophyll content (SPAD) Composition 1 30.24c 9.72c 28.32c Composition 2 35.43b 10.53b 30.75b Composition 3 36.65b 11.72b 32.42b Composition 4 38.54a 12.98ab 33.48ab Composition 5 37.24ab 12.35ab 33.98ab Composition 6 38.87a 13.87a 35.56a Composition 7 37.43ab 13.02ab 34.62ab Composition 8 37.24b 12.58ab 33.25ab Composition 9 36.45b 11.72b 32.12b Composition 10 35.38b 11.95b 31.85b

[0111] Note: Different lowercase letters indicate significant differences among treatments (P<0.05).

[0112] As shown in Table 2, plant height, stem thickness and chlorophyll content were increased after the application of cyclic lipopeptide, among which plant height increased by 17.00%-38.54%, and the plant height of composition 6 treatment was significantly increased by 38.54% compared with the treatment of composition 1; stem thickness increased by 8.33%-42.69%, and the stem thickness of composition 6 treatment was significantly increased by 42.69% compared with the treatment of composition 1; chlorophyll content increased by 8.58%-25.56%, and the chlorophyll content of composition 6 treatment was significantly increased by 25.56% compared with the treatment of composition 1; compared with the treatment of composition 1, the plant height, stem thickness and chlorophyll content of cucumbers treated with composition 4-8 increased more significantly, and the plant height, stem thickness and chlorophyll content of cucumbers treated with composition 2-3 and composition 9-10 increased significantly, but the increase effect was not as good as that of composition 4-8. This shows that the application of cyclic lipopeptides can promote the growth of cucumbers and improve the plant height, stem thickness, chlorophyll content and other indicators of cucumbers.

[0113] Experimental Example 2 Effect on Corn

[0114] (1) Test crops: corn, the variety is Nonghua 238.

[0115] (2) Test agents: Composition 1, compositions 4-8, and compositions 11-35.

[0116] (3) Experimental design: The experimental treatment settings are as follows:

[0117] The composition was diluted 500 times and sprayed on the leaves, 30 m in each plot. 2 Each treatment was repeated 3 times, and the treatment was applied once every other week for a total of 3 times. 10 days after the third application, the plant height, stem diameter, 1000-grain weight and yield growth index of corn samples in each plot were measured, and the results are shown in Table 3.

[0118] Table 3

[0119]

[0120]

[0121] Note: Different lowercase letters indicate significant differences among different treatments (P<0.05).

[0122] As can be seen from Table 3, after applying composition 11-35, the plant height, stem diameter, 1000-grain weight and yield of corn were all improved, among which the plant height increased by 2.92%-15.21%, and the plant height of the composition 13 treatment was significantly increased by 15.21% compared with the composition 1 treatment; the stem diameter increased by 6.48%-31.77%, and the stem diameter of the composition 13 treatment was significantly increased by 31.77% compared with the composition 1 treatment; the 1000-grain weight increased by 1.57%-10.29%, and the 1000-grain weight of the composition 13 treatment was significantly increased by 10.29% compared with the composition 1 treatment; the yield increased by 3.13%-9.71%, and the yield of the composition 13 treatment was significantly increased by 9.71% compared with the composition 1 treatment. This shows that the application of a composition containing cyclic lipopeptides and linear lipopeptides can significantly promote the growth of corn, increase the plant height, stem diameter, 1000-grain weight and yield of corn, and the effect of the composition is significantly better than that of a single application. The growth-promoting effect of the composition formed by the combination of cyclic lipopeptide surfactant, fengycin, iturin, lichenin, fusarium, echinocandin, octapeptide, polymyxin, lecithin and linear lipopeptides is significantly better than that of a single application.

[0123] Experimental Example 3 Effect on Pasture

[0124] (1) Test crops: highland barley, whose variety is black six-angle barley, alfalfa, whose variety is Gannong No. 5, sainfoin, whose variety is Caucasian sainfoin, and oat grass, whose variety is Qingyin No. 2.

[0125] (2) Test agents: Composition 4, Composition 11, Composition 13 and Composition 1

[0126] (3) Experimental design: The experimental treatment settings are as follows:

[0127] The composition was diluted 500 times and sprayed on the leaves, 30 m in each plot. 2 Each treatment was repeated 3 times, and the treatment was applied once every other week for a total of 3 times. Ten days after the third application, the plant height of highland barley, alfalfa, sainfoin, and oat grass samples in each plot was measured, and the yield and fresh weight were measured at harvest. The results are shown in Table 4.

[0128] Table 4

[0129]

[0130]

[0131] Note: Different lowercase letters indicate significant differences among treatments (P<0.05).

[0132] As shown in Table 4, after applying the composition 13, the plant height and yield of the forage grass increased, among which the plant height of highland barley increased by 16.96% and the yield increased by 5.52%; the plant height of alfalfa increased by 20.50%, and the fresh weight of the aboveground part increased by 14.28% per square meter; the plant height of red bean grass increased by 22.88%, and the fresh weight of the aboveground part increased by 10.68% per square meter; the plant height of oat grass increased by 8.44%, and the fresh weight of the aboveground part increased by 7.14% per square meter. This shows that compared with the single application of cyclic lipopeptide or linear lipopeptide or the single application of water, the application of the composition can significantly promote the growth of forage grass, increase the plant height and yield of forage grass, and at the same time, it shows that the growth-promoting effect of cyclic lipopeptide and linear lipopeptide also works on forage grass.

[0133] Experimental Example 4 Effects on Rice, Wheat, Soybean, Tomato, Chinese Cabbage, Lettuce, Sweet Potato, Strawberry and Watermelon

[0134] 1. Test crops: rice, its variety is Hanyou 73, wheat, its variety is Zhongmai 175, soybean, its variety is Zhonghuang 37, tomato, its variety is Jinpeng No. 1, Chinese cabbage, its variety is Lugouqiao brand new cabbage, lettuce, its variety is cream lettuce, sweet potato, its variety is Yanshu 29, strawberry, its variety is Hongyan, watermelon, its variety is Tianwang.

[0135] 2. Test agents: Composition 4, Composition 11, Composition 13 and Composition 1

[0136] 3. Experimental design: The experimental treatment settings are as follows:

[0137] The composition was diluted 500 times and sprayed on the leaves, 30 m in each plot. 2 Each treatment was repeated 3 times, and the application was performed once every other week for a total of 3 times. The yield of each crop was measured at harvest, and the results are shown in Table 5.

[0138] Table 5

[0139]

[0140]

[0141] Note: Different lowercase letters indicate significant differences among treatments (P<0.05).

[0142] As shown in Table 5, compared with the application of composition 1, the yields of rice, wheat, soybean, tomato, Chinese cabbage, lettuce, sweet potato, strawberry and watermelon were increased after the application of composition 13, among which the yield of rice increased by 5.49%; the yield of wheat increased by 7.16%; the yield of soybean increased by 10.98%; the yield of tomato increased by 9.79%; the yield of Chinese cabbage increased by 7.81%; the yield of lettuce increased by 7.59%; the yield of sweet potato increased by 9.05%; the yield of strawberry increased by 7.74%; and the yield of watermelon increased by 7.83%. The test results show that the composition obtained by compounding the two lipopeptides has an increase in yield of various crops, and the increase in yield is more than 5%. In addition, it can be seen from composition 4, composition 11 and composition 13 that the combination of cyclic lipopeptides and linear lipopeptides can significantly increase the yield of various crops. Therefore, we infer that the composition obtained by compounding the two lipopeptides can promote the growth of various crops and increase crop yields. The growth-promoting effects of cyclic lipopeptides and linear lipopeptides are universal.

[0143] The above is only the preferred embodiment of the present application, and does not limit the present application in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present application without departing from the technical solution of the present application still falls within the protection scope of the technical solution of the present application.

Claims

1. A composition for promoting plant growth, comprising a cyclic lipopeptide and a linear lipopeptide.

2. The composition according to claim 1, wherein The cyclic lipopeptide comprises one or more selected from the group consisting of surfactin, lichenin, iturin family, echinocandins, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or The linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

3. The composition according to claim 1 or 2, wherein the cyclic lipopeptide is 0.01-40%, preferably 0.01-20%; the linear lipopeptide is 0.01-20%, preferably 1-10% by mass percentage in the composition; Preferably, the mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500.

4. The composition according to any one of claims 1 to 3, wherein the composition further comprises an active ingredient and / or an agriculturally acceptable adjuvant; Preferably, the dosage form of the composition comprises one selected from aqueous solution, suspension, granule, powder, seed coating, suspoemulsion and soluble liquid.

5. Use of the composition according to any one of claims 1 to 4 in the preparation of biological bacterial agents, bacterial fertilizers, amino acid-containing water-soluble fertilizers or organic water-soluble fertilizers.

6. Use of linear lipopeptides in promoting plant growth.

7. The use according to claim 6, wherein The linear lipopeptide is obtained by fermentation using Brevibacillus laterus.

8. Use of linear lipopeptides in enhancing cyclic lipopeptides to promote nutrient absorption and thus promote plant growth.

9. The use according to claim 8, wherein The cyclic lipopeptide comprises one or more selected from the group consisting of surfactin, lichenin, iturin family, echinocandins, fengycin, phospholipid inhibitor, octapeptidin, polymyxin and fusaricide; and / or The linear lipopeptide is obtained by fermentation using Brevibacillus laterus; The mass ratio of the cyclic lipopeptide to the linear lipopeptide is 1:0.5-1000, preferably 1:50-500; Preferably, the concentration of the cyclic lipopeptide is 0.01-40wt%, preferably 0.01-20wt%; The linear lipopeptide is 0.01-20wt%, preferably 1-10wt%.

10. A method for promoting plant growth, comprising applying the composition according to any one of claims 1 to 4 to plants to promote nutrient absorption, thereby promoting plant growth.

Images