Composition as well as preparation method and application thereof

Abstract

The invention discloses a composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials: yeast powder, oyster protein peptide, arctic shellfish protein peptide, sea cucumber protein peptide, a yeast extract, hovenia dulcis thunb, tremella fuciformis, grape powder, turmeric and hericium erinaceus powder. According to the scheme, all the components are synergistically used for dispelling the effects of alcohol, the alcohol content in a living body can be effectively reduced, and meanwhile, all the components are natural and free of toxic and side effects, and can be used for large-scale production.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a composition and a preparation method and application thereof. Background Art

[0002] "Wine culture" is an important part of the Chinese nation's food culture. Long-term drinking can cause great harm to the human body. For example, the alcohol and its toxic metabolite acetaldehyde can indirectly or directly cause fatty liver, cirrhosis, hypoglycemia, hyperlipidemia, damage to the central nervous system and other diseases. In order to achieve the purpose of sobering up, people usually take substances containing sedatives, hormones, etc.

[0003] Although taking the above-mentioned drugs can effectively achieve the purpose of sobering up, they are harmful to the human body to a certain extent. Therefore, seeking safe and effective sobering up agents has been one of the hot topics that people have long paid attention to. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a composition that can effectively reduce the alcohol content in blood and achieve the effect of sobering up.

[0005] The invention also provides a method for preparing the composition.

[0006] The present invention also provides the application of the composition.

[0007] The invention also provides a product for sobering up.

[0008] According to one aspect of the present invention, a composition is provided, which comprises the following raw materials: yeast powder, oyster protein peptide, arctic shellfish protein peptide, sea cucumber protein peptide, yeast extract, Hovenia dulcis, Tremella fuciformis, grape powder, turmeric and Hericium erinaceus powder.

[0009] In some embodiments of the present invention, the composition comprises the following raw materials by weight: 0.5-6 parts of yeast powder, 0.1-5 parts of oyster protein peptide, 0.1-5 parts of arctic shell protein peptide, 0.1-4 parts of sea cucumber protein peptide, 0.1-2 parts of yeast extract, 0.01-2 parts of Hovenia dulcis, 0.01-2 parts of Tremella fuciformis, 0.01-2 parts of grape powder, 0.01-2 parts of turmeric and 0.01-1 parts of Hericium erinaceus powder.

[0010] In some embodiments of the present invention, the composition comprises the following raw materials by weight: 1-4 parts of yeast powder, 0.2-3 parts of oyster protein peptide, 0.3-3 parts of arctic shell protein peptide, 0.8-3 parts of sea cucumber protein peptide, 0.2-1 parts of yeast extract, 0.1-1 parts of Hovenia dulcis, 0.1-1 parts of Tremella fuciformis, 0.1-0.8 parts of grape powder, 0.1-1 parts of turmeric and 0.05-0.8 parts of Hericium erinaceus powder.

[0011] In some embodiments of the present invention, the composition comprises the following raw materials by weight: 2-3 parts of yeast powder, 0.5-2 parts of oyster protein peptide, 0.5-2 parts of Arctic shell protein peptide, 1-2 parts of sea cucumber protein peptide, 0.5-0.8 parts of yeast extract, 0.2-0.6 parts of Hovenia dulcis, 0.2-0.5 parts of Tremella fuciformis, 0.2-0.4 parts of grape powder and 0.2-0.5 parts of turmeric, and 0.1-0.4 parts of Hericium erinaceus powder.

[0012] In some embodiments of the present invention, the composition further comprises green kumquat fruit powder.

[0013] In some embodiments of the present invention, the addition ratio of the green kumquat fruit powder to turmeric is (2-4):(2-5).

[0014] In some embodiments of the present invention, the addition ratio of the green kumquat fruit powder to turmeric is 3:(2-5).

[0015] In some embodiments of the present invention, the composition further comprises a sweetener, a filler and / or a lubricant.

[0016] In some embodiments of the present invention, the composition further comprises the following raw materials in the following mass fractions: 5-15 parts of sweetener, 0.1-8 parts of filler and / or 0.01-5 parts of lubricant.

[0017] In some embodiments of the present invention, the composition further comprises the following raw materials in the following mass fractions: 7-12 parts of sweetener, 0.5-5 parts of filler and / or 0.05-3 parts of lubricant.

[0018] In some embodiments of the present invention, the composition further comprises the following raw materials in weight fractions: 8-10 parts of sweetener, 0.8-3 parts of filler and / or 0.1-0.8 parts of lubricant.

[0019] In some embodiments of the present invention, the composition further comprises the following raw materials in the following mass fractions: 9 parts of sweetener, 1.1 parts of filler and / or 0.3 parts of lubricant.

[0020] In some embodiments of the present invention, the sweetener is selected from at least one of dextrose, erythritol, xylitol, arabitol, sorbitol, mannitol, iditol, galactitol, maltitol, isomalt, isomalt and lactitol.

[0021] In some embodiments of the present invention, the filler is microcrystalline cellulose and / or lactose.

[0022] In some embodiments of the present invention, the lubricant is silicon dioxide and / or magnesium stearate.

[0023] According to a second aspect of the present invention, a method for preparing the above-mentioned composition is provided, and the method comprises the following steps: mixing the preparation raw materials.

[0024] According to a third aspect of the present invention, an application of the above composition is proposed, wherein the application is an application in preparing a product for sobering up.

[0025] In some embodiments of the present invention, the product comprises a food or a medicine.

[0026] According to a fourth aspect of the present invention, a product for sobering up is provided, the product comprising the above composition.

[0027] In some embodiments of the present invention, the product comprises a food or a medicine.

[0028] In some embodiments of the present invention, the product may be a yeast polypeptide chewable tablet.

[0029] In some embodiments of the present invention, the yeast polypeptide chewable tablet is prepared by the following method: the above composition is prepared according to a tableting process.

[0030] According to some embodiments of the present invention, at least the following beneficial effects are achieved: The present invention provides a composition for sobering up, comprising yeast powder, oyster protein peptide, arctic shellfish protein peptide, sea cucumber protein peptide, yeast extract, Hovenia dulcis, Tremella fuciformis, grape powder, turmeric and Hericium erinaceus powder, wherein:

[0031] Yeast powder contains active acetaldehyde dehydrogenase ALDH and alcohol dehydrogenase ADH;

[0032] Oyster protein peptide is a polypeptide hydrolyzed from oyster protein. It is rich in amino acids, taurine, vitamins and other nutrients. It has a good protective effect on chemical liver damage, can significantly reduce liver damage and transaminase content, and reduce the degree of liver cell damage.

[0033] Arctic scallop is a polypeptide hydrolyzed from Arctic scallop protein. It is rich in minerals such as calcium, iron, zinc, and selenium. It also contains a variety of marine active peptides, polypeptides, enzymes, short peptides and other biologically active substances. It has the effects of nourishing the stomach and spleen, and promoting diuresis and reducing swelling.

[0034] Sea cucumber protein peptide is a polypeptide made by hydrolysis of sea cucumber protein. It can help supplement the nutrients needed by the body, improve human immunity, lower blood pressure, lower blood lipids, reduce the formation of blood clots, and help protect cardiovascular and cerebrovascular health; at the same time, sea cucumber peptide has a strong antioxidant effect, can remove free radicals, delay cell aging, protect physical health, and can significantly maintain and improve the activity of alcohol dehydrogenase ADH, acetaldehyde dehydrogenase ALDH, and antioxidant dismutase, accelerate alcohol metabolism, and reduce oxygen free radical damage;

[0035] Hovenia dulcis contains a lot of glucose and calcium malate, which can accelerate the metabolism of alcohol and thus play a role in sobering up;

[0036] Grape powder and Tremella fuciformis are rich in antioxidants and can synergistically accelerate alcohol metabolism;

[0037] Turmeric helps protect the liver from damage while speeding up alcohol metabolism;

[0038] Hericium erinaceus powder contains rich protein, amino acids and polysaccharides, has a neuroprotective effect, and can be used in conjunction with other components to sober up. The scheme of the present invention can effectively reduce the alcohol content in the body by using the above components in conjunction with each other to sober up. At the same time, all ingredients are derived from nature, have no toxic side effects, and can be used for large-scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0040] Figure 1 This is a graph showing the results of the zebrafish movement experiment in the test example of the present invention, where "*" means p < 0.05, "**" means p < 0.01, and "***" means p < 0.001;

[0041] Figure 2 This is a diagram of the zebrafish movement experiment detection results in the test example of the present invention. DETAILED DESCRIPTION

[0042] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present invention.

[0043] Experimental instruments and reagents

[0044] Zebrafish behavior trajectory tracking system (DanioVision) (BOM-ZDAN-OBS, Nordus (Beijing) Information Technology Co., Ltd.), anhydrous ethanol (CAS: 64-17-5), RU21 (imported from the United States).

[0045] Example 1

[0046] In this embodiment, a composition is prepared, which contains the following raw materials in parts by weight: 2 parts of yeast powder, 1 part of oyster protein peptide, 1 part of arctic shell protein peptide, 1 part of sea cucumber protein peptide, 0.8 parts of yeast extract, 0.4 parts of Hovenia dulcis, 0.4 parts of Tremella fuciformis, 0.3 parts of grape powder, 0.2 parts of turmeric, 0.2 parts of Hericium erinaceus powder, 9 parts of sorbitol, 1.1 parts of microcrystalline cellulose, 0.3 parts of kumquat fruit powder, and 0.3 parts of magnesium stearate.

[0047] The preparation method of the composition is as follows: the above components are mixed to obtain the composition.

[0048] Example 2

[0049] In this embodiment, a composition is prepared, which contains the following raw materials in parts by weight: 2 parts of yeast powder, 1 part of oyster protein peptide, 0.5 parts of arctic shellfish protein peptide, 2 parts of sea cucumber protein peptide, 0.8 parts of yeast extract, 0.2 parts of Hovenia dulcis, 0.5 parts of Tremella fuciformis, 0.4 parts of grape powder, 0.5 parts of turmeric, 0.1 parts of Hericium erinaceus powder, 9 parts of sorbitol, 1.1 parts of microcrystalline cellulose, 0.3 parts of kumquat fruit powder, and 0.3 parts of magnesium stearate.

[0050] The preparation method of the composition is the same as that of Example 1.

[0051] Example 3

[0052] In this embodiment, a composition is prepared, which contains the following raw materials in parts by weight: 3 parts of yeast powder, 0.5 parts of oyster protein peptide, 2 parts of arctic shell protein peptide, 1.5 parts of sea cucumber protein peptide, 0.5 parts of yeast extract, 0.6 parts of Hovenia dulcis, 0.2 parts of Tremella fuciformis, 0.2 parts of grape powder, 0.3 parts of turmeric, 0.4 parts of Hericium erinaceus powder, 9 parts of sorbitol, 1.1 parts of microcrystalline cellulose, 0.3 parts of kumquat fruit powder, and 0.3 parts of magnesium stearate.

[0053] The preparation method of the composition is the same as that of Example 1.

[0054] Example 4

[0055] This example prepares a yeast polypeptide chewable tablet, and the specific process is as follows:

[0056] A chewable tablet containing the above composition (the composition prepared in Examples 1-3, respectively) is prepared according to a dry granulation tableting process, and the preparation steps are as follows:

[0057] (1) Weigh each component in proportion.

[0058] (2) Premixing: Place all components in a mixer and premix for 5 minutes.

[0059] (3) Granulation: The mixed powder premixed in step (2) above was used to prepare granules using a dry granulator. The conditions for dry granulation were as follows: screw speed 20 rpm, roller speed 8 rpm, granulation speed 150 rpm, roller pressure 3 MPa, and screen aperture 1.0 mm.

[0060] (4) Tableting: tabletting is performed using a tablet press to obtain chewable yeast polypeptide tablets.

[0061] Comparative Example 1

[0062] This comparative example prepares a composition, which is different from Example 1 in that yeast powder is missing.

[0063] The preparation method of the composition is the same as that of Example 1.

[0064] Comparative Example 2

[0065] This comparative example prepares a composition, which is different from Example 1 in that oyster protein peptide is missing.

[0066] The preparation method of the composition is the same as that of Example 1.

[0067] Comparative Example 3

[0068] This comparative example prepares a composition which differs from Example 1 in that it lacks the arctic protein peptide.

[0069] The preparation method of the composition is the same as that of Example 1.

[0070] Comparative Example 4

[0071] This comparative example prepares a composition, which is different from Example 1 in that oyster protein peptide and arctic protein peptide are missing.

[0072] The preparation method of the composition is the same as that of Example 1.

[0073] Comparative Example 5

[0074] This comparative example prepares a composition, which is different from Example 1 in that it lacks sea cucumber protein peptide.

[0075] The preparation method of the composition is the same as that of Example 1.

[0076] Comparative Example 6

[0077] This comparative example prepares a composition, which is different from Example 1 in that Hovenia dulcis is missing.

[0078] The preparation method of the composition is the same as that of Example 1.

[0079] Comparative Example 7

[0080] This comparative example prepares a composition, which is different from Example 1 in that Tremella fuciformis is missing.

[0081] The preparation method of the composition is the same as that of Example 1.

[0082] Comparative Example 8

[0083] This comparative example prepares a composition which differs from Example 1 in that grape powder is missing.

[0084] The preparation method of the composition is the same as that of Example 1.

[0085] Comparative Example 9

[0086] This comparative example prepares a composition, which is different from Example 1 in that Tremella fuciformis and grape powder are missing.

[0087] Comparative Example 10

[0088] This comparative example prepares a composition which differs from Example 1 in that turmeric is absent.

[0089] The preparation method of the composition is the same as that of Example 1.

[0090] Comparative Example 11

[0091] This comparative example prepares a composition, which is different from Example 1 in that Hericium erinaceus powder is missing.

[0092] The preparation method of the composition is the same as that of Example 1.

[0093] Test example

[0094] This test example tests the hangover-removing performance of the compositions prepared in Examples 1-3 and Comparative Examples 1-11.

[0095] 1. Blood ethanol concentration test of mice at different times after drinking to verify the hangover-relieving performance of the composition

[0096] (1) Grouping and modeling

[0097] Thirty male SD mice were randomly divided into 16 groups, with 5 mice in each group, namely, a blank control group, a model group, a composition prepared in Example 1-3 + a model group, and a composition prepared in Comparative Example 1-11 + a model group.

[0098] Construction of the drunkenness model group: SD mice were gavaged with 10 mL / (kg·bw) of 50% (V / V) ethanol, and the drunkenness model group was constructed 30 minutes later. The control group was gavaged with an equal amount of pure water.

[0099] (2) Dosing by group

[0100] After the model was constructed, the drug was administered, and the dosage was converted according to the recommended human dosage (the proposed human dosage was 5 g / d, and the human body weight was 70 kg, which was calculated as 5 times the recommended human intake). The composition prepared in Example 1-3 + model group and the composition prepared in Comparative Example 1-11 + model group were administered with 357.1 mg / Kg of the corresponding composition. Blood was collected by orbital blood sampling at 3 hours after administration. The ethanol concentration in the serum was detected, and the test results were averaged.

[0101] Table 1

[0102]

[0103]

[0104] The results are shown in Table 1. It can be seen from Table 1 that the composition prepared by the scheme of the present invention can effectively promote the metabolism of ethanol, reduce the content of ethanol in the blood, effectively alleviate the symptoms of drunkenness, and has a hangover effect.

[0105] 2. Zebrafish exercise experiment to detect the hangover-relieving performance of the composition

[0106] Drunkenness is actually acute ethanol poisoning, which excites the human brain and causes abnormal behavior. After ethanol enters the human body, 90% enters the liver for metabolism. Alcohol dehydrogenase (ADH) can oxidize it into acetaldehyde. Under the catalysis of acetaldehyde deoxygenase (ALDH), acetaldehyde is converted into acetic acid, and finally into carbon dioxide and water. Under the action of the two enzymes ADH and ALDH, ethanol can be metabolized by the body to avoid causing damage to the body. Since zebrafish have a digestive system similar to that of humans, such as liver, intestines, etc., digestion and nutrient absorption and transportation are highly similar to those of humans. When ethanol is excessively ingested, a large amount of acetaldehyde is produced through metabolism, causing acetaldehyde to accumulate in the body and causing drunkenness. The total movement distance is detected to evaluate the alcohol sobering effect of the sample, and the movement trajectory of zebrafish is analyzed using a behavioral analyzer. The total movement distance of drunk zebrafish is significantly increased compared with normal zebrafish. The positive control drug for the experiment is RU21.

[0107] The specific experimental methods are as follows:

[0108] (1) Composition concentration screening

[0109] Wild AB zebrafish were divided into 6 groups, each with 30 fish. They were placed in different concentrations (0.12, 0.6, 3, 15, and 75 mg / mL, respectively) of the composition of Example 1. After 3 hours, the number of deaths and mortality were counted.

[0110] Table 2

[0111]

[0112] The results are shown in Table 2. It can be seen from Table 2 that 0.6 mg / mL is the best addition ratio.

[0113] (2) Verification of hangover-relief performance

[0114] Construction of zebrafish hangover model: Wild AB strain zebrafish (3 days after fertilization) were treated with ethanol, and 20 μL of alcohol solution was injected into the mouth of each zebrafish with a syringe. The alcohol concentration was controlled to be 0.05%, so that the zebrafish ingested excessive ethanol to prepare the zebrafish hangover model.

[0115] Grouping: 4 groups were set up, namely a model control group, a blank control group, a treatment group with the composition in Example 1 at a final concentration of 0.6 mg / mL, and a positive control drug group, wherein the model control group was added with a zebrafish hangover model, the blank control group was added with wild AB strain zebrafish (3 days after fertilization), the treatment group with the composition in Example 1 at a final concentration of 0.6 mg / mL was added with 0.6 mg / mL of the composition in Example 1 and a zebrafish hangover model, the positive control group was added with 0.6 mg / mL of RU21 and a zebrafish hangover model, and the sample size of the zebrafish experiment was 30 per well, and 1 well per group.

[0116] Adult fish rearing and breeding methods followed standard rearing and breeding methods.

[0117] Judgment basis:

[0118] On the basis of the effectiveness of the experiment, the total movement distance of the model control group was significantly reduced compared with the blank control group (P<0.05), which means that the modeling was successful.

[0119] Compared with the model control group, the total exercise distance of the sample test group was significantly increased (P<0.05), indicating that the sample has the effect of sobering up.

[0120] Data processing method:

[0121] SPSS26 software was used for analysis, GraphPad Prism8 was used for drawing, and data statistics were expressed as mean ± standard deviation. SPSS software was used to test the normal distribution of the experimental results. If the data conformed to the normal distribution, the T parameter test or analysis of variance (ANOVA) was used for significance. If it did not conform to the normal distribution, a non-parametric test was used (the result P value <0.05 indicated a statistically significant difference).

[0122] The results are as follows Figure 1 and Figure 2 As shown in the figure, it can be seen that the composition prepared by the scheme of the present invention can be effectively used for zebrafish to sober up, and has a certain sobering up effect.

[0123] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A composition, characterized in that The composition comprises the following raw materials: yeast powder, oyster protein peptide, arctic shellfish protein peptide, sea cucumber protein peptide, yeast extract, Hovenia dulcis fruit, Tremella fuciformis, grape powder, turmeric and Hericium erinaceus powder.

2. The composition according to claim 1, characterized in that The composition comprises the following raw materials by weight: 0.5-6 parts of yeast powder, 0.1-5 parts of oyster protein peptide, 0.1-5 parts of arctic shell protein peptide, 0.1-4 parts of sea cucumber protein peptide, 0.1-2 parts of yeast extract, 0.01-2 parts of Hovenia dulcis, 0.01-2 parts of Tremella fuciformis, 0.01-2 parts of grape powder, 0.01-2 parts of turmeric and 0.01-1 parts of Hericium erinaceus powder.

3. The composition according to claim 2, characterized in that The composition comprises the following raw materials by weight: 1-4 parts of yeast powder, 0.2-3 parts of oyster protein peptide, 0.3-3 parts of arctic shell protein peptide, 0.8-3 parts of sea cucumber protein peptide, 0.2-1 parts of yeast extract, 0.1-1 parts of Hovenia dulcis, 0.1-1 parts of Tremella fuciformis, 0.1-0.8 parts of grape powder, 0.1-1 parts of turmeric and 0.05-0.8 parts of Hericium erinaceus powder.

4. The composition according to claim 1, characterized in that The composition also includes green kumquat fruit powder; Preferably, the addition ratio of the green kumquat fruit powder to turmeric is (2-4):(2-5).

5. The composition according to claim 1, characterized in that The composition may also include a sweetener, a filler and / or a lubricant.

6. The composition according to claim 5, characterized in that The composition further comprises the following raw materials in mass fractions: 5-15 parts of a sweetener, 0.1-8 parts of a filler and / or 0.01-5 parts of a lubricant; Preferably, the composition further comprises the following raw materials in the following mass fractions: 7-12 parts of sweetener, 0.5-5 parts of filler and / or 0.05-3 parts of lubricant.

7. The composition according to claim 5, characterized in that The sweetener is selected from at least one of dextrose, erythritol, xylitol, arabitol, sorbitol, mannitol, iditol, galactitol, maltitol, isomalt, isomalt and lactitol; and / or, the filler is microcrystalline cellulose and / or lactose; And / or, the lubricant is silicon dioxide and / or magnesium stearate.

8. A method for preparing the composition according to any one of claims 1 to 7, characterized in that: The preparation raw materials are mixed to obtain the product.

9. Use of the composition according to any one of claims 1 to 7 in the preparation of a product for sobering up.

10. A product for sobering up, characterized in that: The product comprises the composition according to any one of claims 1 to 7.

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