Hydrophobic needle solution for improving facial fine wrinkles or fullness and preparation method of hydrophobic needle solution

Abstract

The invention discloses a hydro-smooth needle solution for improving facial fine wrinkles or fullness and a preparation method thereof. The hydro-smooth needle solution comprises the following components: a hyaluronic acid solution; the hydroxyapatite, the L-carnosine and the chitosan are added into the hyaluronic acid solution. The preparation method comprises the following steps: respectively providing the hyaluronic acid solution, the hydroxyapatite powder, the L-carnosine and the chitosan, and uniformly mixing in a two-way tube to obtain the hydro-optical needle solution. By adding the L-carnosine into the hydro-optical needle solution provided by the invention, membrane lipid and protein can be prevented from being damaged by oxidative stress, meanwhile, saccharification reaction can be prevented, and biological macromolecules (such as collagen) can be protected from being damaged by ketose or aldose under the condition of oxidative stress; in addition, chitosan molecules added into the hydro-optical needle solution serve as a substrate, mechanical support can be provided for newborn collagen, stable space support can be provided for the newborn collagen and regenerated tissue according to the formula, and therefore the lasting anti-aging effect is achieved.

Description

Technical Field

[0001] The present invention relates to the field of medical cosmetology technology, and more particularly to a water-light needle solution for improving facial fine lines or enhancing facial fullness and a preparation method thereof. Background Art

[0002] Skin aging is a complex process that is regulated by both genetic and environmental factors (Farage MA, Miller KW, Elsner P, et al. Intrinsic and extrinsic factors in skin ageing: a review. Int J Cosmet Sci. 2008; 30: 87–95.).

[0003] Among them, genetic factors mainly include antioxidant capacity, anti-inflammatory factors and genetic aging patterns; while environmental factors mainly include environmental exposure (ultraviolet radiation, dry environment, extreme low temperature, etc.), lifestyle, eating habits, stress and other factors (Fisher GJ, Kang S, Varani J, et al. Mechanisms of photoaging and chronological skin aging. Arch Dermatol. 2002; 138: 1462–70. Benavides F, Oberyszyn TM, VanBuskirk AM et al. The hairless mouse in skin research, JDermatol Sci. 2009 Jan; 53 (1): 10-8.). Due to the differences in genetic and environmental factors, the aging process of people is also very different, and the subdivision of aging indicators is different for each person. Therefore, when doing anti-aging activities for the skin, genetic and environmental factors should be fully considered, so as to comprehensively evaluate the individual's skin aging status and aging pattern, and provide targeted strategies for personalized treatment.

[0004] Sodium hyaluronate is widely present in human tissues and cells. It is an important component of the extracellular matrix and the proteoglycan chain that transmits cell information. Since 1970, sodium hyaluronate has been widely used in the skin care industry because it can lock in moisture and increase skin elasticity. Among them, delivering sodium hyaluronate directly to the dermis through microneedle injection has been proven to be an effective method to improve the overall quality of the skin. In the dermis, sodium hyaluronate can hydrate the skin and support fibroblasts to function better.

[0005] Studies have shown that almost all types of sodium hyaluronate formulas for facial improvement can improve facial skin quality to a certain extent. They play a role in multiple dimensions such as skin hydration, firmness, brightness, texture, gloss and elasticity, but the improvement effect is limited and still needs to be further improved. Type I collagen is the main component of the dermis, which constitutes collagen fibers and improves the elasticity and firmness of the skin. However, with age, the synthesis rate of type I collagen decreases, resulting in the breakage and reduction of collagen fibers, thus forming wrinkles. However, the current sodium hyaluronate formula is less effective in stimulating the regeneration of type I collagen.

[0006] CaHA has been used in medical aesthetics for more than 20 years and is a relatively safe collagen stimulator (Kadouch JA. Calcium hydroxylapatite: a review on safety and complications, J Cosmet Dermato. (2017) 16: 152-61.10.1111 / jocd.12326). However, the particle size of hydroxyapatite (CaHA) currently available on the market is generally 20-50 μm, and it uses a blunt needle to deliver the drug, but this places high demands on doctors and is difficult to operate (Lorence ZP, Black JM, Cheung JS, et al., Skin Tightening with hyperdilute CaHA: Dilution Practices and Practical guidance for clinical practice. Aesthet Surg J. 2022 Jan 1; 42 (1): NP29-NP37). Summary of the invention

[0007] The purpose of the present invention is to address the problems that the current hyaluronic acid formula used to improve facial skin quality has limited improvement effects and needs to be further improved, and is weak in stimulating the regeneration of type I collagen. At the same time, the current hydroxyapatite delivery method has problems such as high operational difficulty. The present invention proposes a water light injection solution that can be used to improve facial fine lines or fullness, which solves the above problems.

[0008] To achieve the above object, the present invention is implemented by the following technical solutions:

[0009] The present invention provides a water light injection solution that can be used to improve facial fine lines or fullness, and the water light injection solution includes the following components:

[0010] Hyaluronic acid solution;

[0011] Hydroxyapatite powder, which is added to the hyaluronic acid solution;

[0012] L-carnosine, which is added to the hyaluronic acid solution;

[0013] and chitosan, which is added to the hyaluronic acid solution.

[0014] Furthermore, a hyaluronic acid solution can be used to improve fine lines or fullness of the face: the amount of L-carnosine added to the hyaluronic acid solution is 1.0-2.0 mg / ml.

[0015] Furthermore, a hyaluronic acid injection solution can be used to improve fine lines or fullness of the face: the amount of chitosan added to the hyaluronic acid solution is 0.1-1.0 mg / ml.

[0016] Furthermore, a water light injection solution can be used to improve fine lines or fullness of the face: the particle size of the hydroxyapatite powder is 5.0 to 15.0 μm.

[0017] Further, a water light injection solution can be used to improve fine lines or fullness of the face: the water light injection solution comprises the following components: a hyaluronic acid solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution;

[0018] Among them, the concentration of the hyaluronic acid solution is 3.0-6.0 mg / ml, its molecular weight does not exceed 1600000, and its viscosity coefficient does not exceed 10000 mPa.S; the added amount of the hydroxyapatite powder in the hyaluronic acid solution is 40.0-60.0 mg / ml; the hyaluronic acid solution with this concentration and viscosity coefficient can be used to improve facial fine lines.

[0019] Further, a water light injection solution can be used to improve fine lines or fullness of the face: the water light injection solution comprises the following components: a hyaluronic acid solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution;

[0020] The concentration of the hyaluronic acid solution is 18.0-22.0 mg / ml, the molecular weight is 1600000-3000000, and the viscosity coefficient is 150000-600000 mPa.S; the amount of hydroxyapatite powder added to the hyaluronic acid solution is 90.0-110.0 mg / ml;

[0021] The hydrating solution with this concentration and viscosity coefficient can be used to enhance facial fullness and improve facial highlights.

[0022] The present invention also provides a method for preparing a hyaluronic acid solution that can be used to improve fine lines or fullness on the face, the method comprising the following steps: providing a hyaluronic acid solution, hydroxyapatite powder, L-carnosine and chitosan respectively, and then mixing them in a double-pass tube to obtain a hyaluronic acid solution.

[0023] The present invention also provides a use of a water-light needle solution that can be used to improve facial fine lines or fullness. The water-light needle solution is used to stimulate the regeneration of skin collagen and elastin, improve facial fine lines, and enhance facial fullness.

[0024] Beneficial effects of the present invention:

[0025] (1) L-carnosine is a natural dipeptide composed of β-alanine and histidine, which has antioxidant properties. The addition of L-carnosine to the hyaluronic acid solution provided by the present invention can protect membrane lipids and proteins from damage by oxidative stress, while also preventing glycation reactions and protecting biological macromolecules (such as collagen) from damage by ketose or aldose under conditions of oxidative stress. In addition, the chitosan molecules added to the hyaluronic acid solution of the present invention serve as a base, which can provide mechanical support for the newly formed collagen. The formula of the present invention can provide a stable spatial support for the newly formed collagen and regenerated tissues, thereby achieving a lasting anti-aging effect.

[0026] (2) The water light needle solution provided by the present invention can better stimulate the regeneration of skin and elastin, and achieve the effect of deeply improving facial fine lines. The interactive synergy between hyaluronic acid, hydroxyapatite, L-carnosine and chitosan in the formula of the present invention significantly promotes collagen regeneration, wherein chitosan can stabilize the structure and promote stem cell regeneration; L-carnosine can provide antioxidant effects; hyaluronic acid, as an effective matrix, plays a role in multiple dimensions such as moisturizing, hydration, firmness, brightness, texture, gloss and elasticity, and can also stabilize the spatial structure of the tissue of new collagen, providing a long-term anti-aging effect; hydroxyapatite can further stimulate the regeneration of collagen, and after compounding with other components to form the water light needle solution of the present invention, the original hydroxyapatite administration operation difficulty is greatly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0028] Figure 1 The results of the water light needle solution of Example 1, Example 2 and Comparative Example 1 on the collagen regeneration ability of mouse skin are shown in the figure;

[0029] Figure 2 The figures are the results of improvement of facial wrinkles after the subjects were treated with the water light needle solution of Example 1 and Comparative Example 1 respectively. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1

[0032] This embodiment 1 provides a water light needle solution that can be used to improve facial fine lines, characterized in that the water light needle solution includes the following components: a hyaluronic acid aqueous solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution;

[0033] Wherein, the concentration of the hyaluronic acid aqueous solution is 4.0 mg / ml, its molecular weight is no more than 1600000, and its viscosity coefficient is no more than 10000 mPa.S;

[0034] Among them, 50.0 mg of hydroxyapatite powder (particle size of 5.0-15.0 μm), 1.5 mg of L-carnosine and 0.5 mg of chitosan are added to each milliliter of hyaluronic acid solution; the hyaluronic acid solution with this concentration and viscosity coefficient can be used to improve fine lines on the face.

[0035] Preparation: The preparation method of the hyaluronic acid solution for improving facial fine lines provided in the above-mentioned Example 1 comprises the following steps: providing hyaluronic acid solution, hydroxyapatite powder, L-carnosine and chitosan respectively, and then mixing them in a double-pass tube to obtain the hyaluronic acid solution.

[0036] Purpose: The water light needle solution provided in the above Example 1 is used to stimulate the regeneration of skin collagen and elastin and improve facial fine lines.

[0037] Compared with commercially available hyaluronic acid products (hyaluronic acid solution with a concentration of 10.0 to 18.0 mg / ml), the hyaluronic acid injection solution provided in Example 1 has a lower concentration and lower viscosity. After being injected into the skin, it has stronger fluidity than other products and can better penetrate into the breaks in the dermis. The fine lines become lighter in visual effect, and it also has the effect of lifting and plumping the face.

[0038] Example 2

[0039] This embodiment 2 provides a water light needle solution that can be used to enhance facial fullness, characterized in that the water light needle solution comprises the following components: a hyaluronic acid aqueous solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution;

[0040] Wherein, the concentration of the hyaluronic acid aqueous solution is 20.0 mg / ml, the molecular weight thereof is 1,600,000 to 3,000,000, and the viscosity coefficient thereof is 150,000 to 600,000 mPa.S;

[0041] Wherein, 100.0 mg of hydroxyapatite powder (particle size of 5.0-15.0 μm), 2.0 mg of L-carnosine and 1.0 mg of chitosan are added to each milliliter of hyaluronic acid solution;

[0042] The hydrating solution with this concentration and viscosity coefficient can be used to enhance facial fullness and improve facial highlights.

[0043] Preparation: The method for preparing the hyaluronic acid solution for enhancing facial fullness provided in the above-mentioned Example 2 comprises the following steps: providing hyaluronic acid solution, hydroxyapatite powder, L-carnosine and chitosan respectively, and then mixing them in a double-pass tube to obtain the hyaluronic acid solution.

[0044] Use: The water light injection solution provided in the above Example 2 is used to enhance facial fullness and improve facial highlights.

[0045] Compared with commercially available hyaluronic acid products (with a concentration of 10.0 to 18.0 mg / ml hyaluronic acid solution), the hyaluronic acid injection solution provided in Example 2 has a higher concentration and higher viscosity. After being injected into the skin, it has the characteristics of higher concentration, lower fluidity and slower degradation than other products, and has a visual popping effect after injection.

[0046] Comparative Example 1

[0047] Provide a common hyaluronic acid injection solution: the hyaluronic acid injection solution is a 4.0 mg / ml pure hyaluronic acid solution.

[0048] test:

[0049] (1) KH1 knockout mice are a type of hairless mice. They have good characteristics in various processes such as topical medication, UVR exposure, and wound healing, and are more in line with the characteristics of human skin. They are the most widely used hairless mice in skin research (Kim TH, Ananthaswamy HN, Kripke ML, et al. Advantages of using hairless mice versus haired mice to test sunscreen efficacy against photoimmune suppressions. Photochem Photobiol. 2003; 78: 37–42). The water-light injection solutions of Example 1, Example 2, and Comparative Example 1 were tested for their ability to regenerate collagen in mouse skin. The test results are as follows: Figure 1 As shown ( Figure 1 Where a represents Comparative Example 1, b represents Example 1, and c represents Example 2), Figure 1 It can be clearly seen that the water-light needle solutions obtained in Examples 1 and 2 of the present invention can better stimulate the regeneration of skin collagen and elastin than the ordinary water-light needle solution in Comparative Example 1.

[0050] (2) The right face of the subject was treated with the water light needle solution obtained in Example 1 of the present invention, and the left face of the subject was treated with the pure hyaluronic acid water light needle solution provided in Comparative Example 1. 3.0 ml of the water light needle solution was injected into each side each time. The interval between each treatment was 4 weeks. After two treatments, the changes in fine lines on the left and right faces were compared. The changes in fine lines before and after the injection treatment were recorded with a 3D camera. The results were recorded as follows: Figure 2 As shown, it can be seen that after two treatments, the facial fine lines of all subjects were improved. Before treatment, the wrinkle depth of the subjects was between -0.74 and -0.50 mm; after treatment, the wrinkle depth of the right side of the face of the subjects using the hyaluronic acid hyaluronic acid solution of Example 1 was between -0.38 and -0.12 mm, and the wrinkle depth of the left side of the face using the pure hyaluronic acid hyaluronic acid product of Comparative Example 1 was between -0.57 and -0.22 mm. It can be seen that the hyaluronic acid hyaluronic acid products of Example 1 and Comparative Example 1 can improve facial wrinkles, but the effect of improving facial wrinkles of the present application is obviously better, and the wrinkle depth of the subjects can be significantly reduced.

[0051] (3) The left face of the subject was treated with the water-light needle solution obtained in Example 2 of the present invention, and the right face of the subject was treated with the pure hyaluronic acid water-light needle solution provided in Comparative Example 1. 3.0 ml of the water-light needle solution was injected on each side each time. The interval between each treatment was 4 weeks. After two treatments, the changes in fine lines on the left and right faces were compared. The changes in facial volume and facial highlights before injection and after two treatments were recorded with a 3D camera. The water-light needle solution of Example 2 was injected into the left face of the subject, and the pure hyaluronic acid water-light needle solution of Comparative Example 1 was injected into the right face. The injections were performed once every 4 weeks. One week after the second injection, the facial changes and facial volume changes were detected with a 3D camera:

[0052] The results showed that the highlight point on the right side of the face injected with the pure hyaluronic acid water light needle solution of comparative example 1 was lower than the highlight point on the left side of the face injected with the water light needle solution of example 2, indicating that the water light needle solution of the present invention has the effect of improving facial highlight points;

[0053] At the same time, volume detection shows that in the right side of the face injected with the pure hyaluronic acid water light needle solution of comparative example 1, the hyaluronic acid is concentrated below the zygomatic bone, and there is no obvious volume reduction in the mandibular part; while in the left side of the face injected with the water light needle solution of example 2, the hyaluronic acid is concentrated at the zygomatic bone, and the mandibular part has a more obvious volume reduction, which visually presents the effect that the center of gravity of the right face is lower than that of the left face. Analysis of facial volume shows that the volume of the left face increased by about 2.67ml, while the volume of the same part of the right face increased by about 2.15ml. Combined with the fact that the volume of the left face area is concentrated at the top, the visual effect of the left face being fuller than the right face is presented, which shows that the water light needle solution provided by the present invention has the effect of plumping the face and improving the fullness of the face after injection, and visually reflects the effect of a bang after injection.

[0054] The addition of L-carnosine to the hyaluronic acid solution provided by the present invention can protect membrane lipids and proteins from damage by oxidative stress, and can also prevent glycation reactions, and can protect biological macromolecules from damage by ketose or aldose under conditions of oxidative stress; in addition, the chitosan molecules added to the hyaluronic acid solution of the present invention serve as a base, which can provide mechanical support for the newly formed collagen. The formula of the present invention can provide a stable spatial support for the newly formed collagen and regenerated tissue, thereby achieving a lasting anti-aging effect.

[0055] The above are preferred embodiments of the present invention and are only used to explain the present invention, not to limit the present invention. Any obvious changes or modifications derived from the technical solution of the present invention are still within the protection scope of the present invention.

Claims

1. A water light injection solution that can be used to improve facial fine lines or fullness, characterized in that: The water light needle solution includes the following components: Hyaluronic acid solution; Hydroxyapatite powder, which is added to the hyaluronic acid solution; L-carnosine, which is added to the hyaluronic acid solution; and chitosan, which is added to the hyaluronic acid solution.

2. The water light injection solution for improving facial fine lines or fullness according to claim 1, characterized in that: The amount of L-carnosine added to the hyaluronic acid solution is 1.0-2.0 mg / ml.

3. The water light injection solution for improving facial fine lines or fullness according to claim 1, characterized in that: The amount of chitosan added to the hyaluronic acid solution is 0.1-1.0 mg / ml.

4. The water light injection solution for improving facial fine lines or fullness according to claim 1, characterized in that: The particle size of the hydroxyapatite powder is 5.0 to 15.0 μm.

5. A water light injection solution for improving facial fine lines or fullness according to any one of claims 1 to 4, characterized in that: The water light needle solution includes the following components: A hyaluronic acid solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution; Among them, the concentration of the hyaluronic acid solution is 3.0-6.0 mg / ml, its molecular weight does not exceed 1600000, and its viscosity coefficient does not exceed 10000 mPa.S; the added amount of the hydroxyapatite powder in the hyaluronic acid solution is 40.0-60.0 mg / ml; the hyaluronic acid solution with this concentration and viscosity coefficient can be used to improve facial fine lines.

6. A water light injection solution for improving facial fine lines or fullness according to any one of claims 1 to 4, characterized in that: The water light needle solution includes the following components: A hyaluronic acid solution, and hydroxyapatite powder, L-carnosine and chitosan added to the hyaluronic acid solution; The concentration of the hyaluronic acid solution is 18.0-22.0 mg / ml, the molecular weight is 1600000-3000000, and the viscosity coefficient is 150000-600000 mPa.S; the amount of hydroxyapatite powder added to the hyaluronic acid solution is 90.0-110.0 mg / ml; The hydrating solution with this concentration and viscosity coefficient can be used to enhance facial fullness and improve facial highlights.

7. A method for preparing a water light injection solution for improving facial fine lines or fullness according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: providing hyaluronic acid solution, hydroxyapatite powder, L-carnosine and chitosan respectively, and then mixing them in a double-pass tube to obtain a water light needle solution.

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