Traditional Chinese medicine preparation for improving myocarditis and enhancing immunity as well as preparation method and application of traditional Chinese medicine preparation

Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly provides a traditional Chinese medicine preparation for improving myocarditis and enhancing immunity as well as a preparation method and application thereof. The traditional Chinese medicine preparation comprises the following components in parts by mass: 10-30 parts of beautiful millettia root, 15-30 parts of smallanthus sonchifolius, 10-20 parts of astragalus membranaceus, 10-20 parts of rhodiola rosea, 10-50 parts of agaricus blazei murill, 5-25 parts of angelica sinensis and 5-20 parts of philippine flemingia root. The traditional Chinese medicine preparation comprises beautiful millettia roots, smallanthus sonchifolius, astragalus membranaceus, rhodiola rosea, agaricus blazei murill, angelica sinensis and philippine flemingia roots, the formula is completely derived from natural herbal medicines, the safety is high, clinically acceptable medicine dosage forms can be prepared, and the traditional Chinese medicine preparation is suitable for long-term management and treatment of myocarditis patients and has good practical application value. The traditional Chinese medicine preparation can effectively improve the left ventricular ejection fraction and the left ventricular shortening rate of a mouse and reduce the levels of CK-MB, LDH, AST and TNF-alpha in serum of the mouse.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and more specifically to a traditional Chinese medicine preparation for improving myocarditis and enhancing immunity, and a preparation method and application thereof. Background Art

[0002] Myocarditis is a disease characterized by localized or diffuse inflammatory lesions of the myocardium. Symptoms of myocarditis may include chest pain or tightness, fatigue, dyspnea, arrhythmia, mild fever, etc. Based on the histological changes and clinical manifestations of myocardial biopsy, myocarditis is divided into fulminant myocarditis, acute myocarditis, chronic active myocarditis, and chronic protracted myocarditis. The pathophysiological mechanism of myocarditis is still unclear. The rat enterovirus myocarditis model shows that the virus enters the acutely damaged myocardial cells, causes myocardial necrosis through replication, intracellular antigen exposure and activation of the host immune system, which lasts for weeks to months and is characterized by virus-specific T lymphocyte activation, causing target organ damage. Cytokines such as tumor necrosis factor, IL-1 and IL-6, viral antibodies and myocardial protein activation accelerate myocardial and contractile function damage. In most patients with myocarditis, the virus is cleared by immune response to reduce myocardial damage and complete recovery of left ventricular function. Long-term follow-up results of patients with acute myocarditis showed that the prevalence of dilated cardiomyopathy reached 21% after 3 years. Dilated cardiomyopathy combined with chronic heart failure is the main outcome of late myocarditis. It can be seen that myocarditis is a potentially serious disease that endangers life.

[0003] The causes of myocarditis include infectious and non-infectious factors. Infectious factors include bacteria, fungi, protozoa, parasites, spirochetes, rickettsiae and viruses; non-infectious factors include the following aspects: (1) immune-mediated diseases (allergens, alloantigens, autoantigens); (2) toxicity (drugs, heavy metals, biological toxic substances, physical damage, etc.). The most common cause is viral infection.

[0004] At present, there is an urgent need to find a suitable drug that can effectively relieve the symptoms of myocarditis and improve the myocardial function of patients. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a Chinese medicine preparation for improving myocarditis and enhancing immunity, and a preparation method and application thereof. The Chinese medicine preparation of the present invention can effectively alleviate the symptoms of myocarditis and improve the myocardial function of patients.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] The Chinese medicinal preparation for improving myocarditis and enhancing immunity comprises, by weight: 10-30 parts of Rhizoma Cynomorii, 15-30 parts of Yacon, 10-20 parts of Astragalus, 10-20 parts of Rhodiola, 10-50 parts of Agaricus blazei, 5-25 parts of Angelica sinensis and 5-20 parts of Tripterygium wilfordii.

[0008] Niu Da Li is a plant of the genus Millettia in the Leguminosae family. Its roots are used as medicine. It can be harvested throughout the year, but the best time to dig the roots is in autumn. Wash, slice and dry in the sun or steam and then dry in the sun.

[0009] Yacon: It is cold in nature, sweet in taste, and enters the liver and spleen meridians. It has the effects of clearing heat and detoxifying, promoting fluid production and relieving cough.

[0010] Astragalus: sweet, warm; Functions and indications: replenishing qi and strengthening the exterior, promoting diuresis and expelling toxins, draining pus, healing sores and promoting tissue regeneration. Used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, chronic diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, edema due to qi deficiency, carbuncle that is difficult to heal, long-term ulcers that do not heal, blood deficiency and jaundice, internal heat and thirst; proteinuria in chronic nephritis, diabetes.

[0011] Rhodiola rosea: cold in nature and flavor; sweet; astringent, belongs to the lung meridian. Functions and indications: replenishing qi and clearing the lungs; improving intelligence and nourishing the heart; astringent and hemostatic; dispersing blood stasis and reducing swelling. Experiments have shown that Rhodiola rosea can improve the blood and oxygen supply to the myocardium, adjust the compliance of the heart and blood vessels, and play a role in adjusting and improving the cardiovascular system.

[0012] Agaricus blazei: It is mild in nature, sweet in taste, and enters the heart, lung, liver, and kidney meridians. It has the effects of improving immunity, strengthening the brain, relieving inflammation, benefiting the kidneys, lowering blood sugar, improving diabetes, lowering cholesterol, enhancing energy, improving arteriosclerosis, and preventing and treating cardiovascular diseases.

[0013] Angelica: sweet, pungent, warm; Functions and indications: nourishing blood and activating blood circulation, regulating menstruation and relieving pain, moistening intestines and relieving constipation. Used for blood deficiency and sallow complexion, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to deficiency and cold, constipation due to dry intestines, rheumatic arthralgia, injuries from falls, carbuncle and ulcer. Wine Angelica activates blood circulation and regulates menstruation. Used for amenorrhea and dysmenorrhea, rheumatic arthralgia, injuries from falls.

[0014] Qianjinba, the function and treatment are: dispelling wind and dampness, removing blood stasis and detoxifying. Treat rheumatic arthralgia, chronic nephritis, traumatic injury, carbuncle, and throat moth.

[0015] Preferably, the Chinese medicine preparation for improving myocarditis and enhancing immunity comprises, by weight: 20 parts of Rhizoma Cynomorii, 20 parts of Yacon, 15 parts of Astragalus, 20 parts of Rhodiola, 30 parts of Agaricus blazei, 15 parts of Angelica sinensis and 10 parts of Rhizoma Cynomorii.

[0016] Preferably, the Chinese medicine preparation for improving myocarditis and enhancing immunity comprises, by weight: 30 parts of Rhizoma Cynomorii, 15 parts of Yacon, 15 parts of Astragalus, 10 parts of Rhodiola, 40 parts of Agaricus Blazei, 5 parts of Angelica Sinensis and 10 parts of Rhizoma Cynomorii;

[0017] Preferably, the Chinese medicine preparation for improving myocarditis and enhancing immunity comprises, by weight: 25 parts of Rhizoma Cynomorii, 30 parts of Yacon, 10 parts of Astragalus, 10 parts of Rhodiola, 20 parts of Agaricus blazei, 10 parts of Angelica sinensis and 20 parts of Tripterygium wilfordii.

[0018] Preferably, the Herba Cynanchii is the Herba Cynanchii root; the Herba Astragali is the root; the Herba Rhodiolae is the Herba Rhodiolae root; the Herba Angelicae Sinensis is the Herba Angelicae Sinensis root; and the Herba Corydalis is the Herba Corydalis root.

[0019] Preferably, the Chinese medicine preparation further comprises auxiliary materials, and the auxiliary materials are used to make the Chinese medicine preparation into one of granules, oral liquid, pills, capsules or decoctions.

[0020] The present invention also provides a method for preparing the Chinese medicine preparation for improving myocarditis and enhancing immunity, comprising:

[0021] The method comprises the following steps: crushing 10-30 parts of Radix Codonopsis pilosulae, 15-30 parts of yacon, 10-20 parts of astragalus, 10-20 parts of rhodiola rosea, 10-50 parts of Agaricus blazei, 5-25 parts of angelica sinensis and 3-10 parts of Radix Codonopsis pilosulae, mixing with a 50 vol% ethanol aqueous solution, boiling for 1-2 hours, taking the decoction and filtering; mixing the filter residue with water, boiling for 1-2 hours, combining the decoction and drying to obtain a traditional Chinese medicine preparation for improving myocarditis and enhancing immunity.

[0022] The present invention also provides the use of the Chinese medicinal preparation for improving myocarditis and enhancing immunity in preparing products for preventing and / or treating myocarditis.

[0023] Preferably, the myocarditis comprises viral myocarditis.

[0024] Preferably, the dosage of the traditional Chinese medicine preparation is 5 to 6 g / kg, preferably 5 g / kg.

[0025] The present invention also provides the use of the Chinese medicinal preparation for improving myocarditis and enhancing immunity in reducing one or more of (1) to (4);

[0026] (1) Serum creatine kinase isoenzyme (CK-MB);

[0027] (2) lactate dehydrogenase (LDH);

[0028] (3) Aspartate aminotransferase (AST);

[0029] (4) Inflammatory factors.

[0030] Preferably, the inflammatory factor includes TNF-α.

[0031] Compared with the prior art, the beneficial effects are: the Chinese medicine preparation of the present invention includes Radix Niu Da Li, Yacon, Astragalus, Rhodiola Rosea, Agaricus Blazei, Angelica and Radix Angelicae Sinensis, the formula is all derived from natural herbs, the safety is high, the toxic and side effects of long-term use are small, and clinically acceptable drug dosage forms can be prepared, which is suitable for the long-term management and treatment of patients with myocarditis, and has good practical application value. After verification by test examples, the Chinese medicine preparation of the present invention can effectively improve the left ventricular ejection fraction and left ventricular shortening rate of mice, reduce the levels of CK-MB, LDH, AST, and TNF-α in mouse serum, and achieve the effects of protecting myocardial cells and enhancing immunity. DETAILED DESCRIPTION

[0032] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] In order to further illustrate the present invention, the following examples are used to explain it in detail. The raw materials used in the following examples and comparative examples of the present invention are all commercially available products; the Herba Cynoglossi used in the examples and comparative examples of the present invention is the Herba Cynoglossi root; the Herba Astragali used is the root; the Herba Rhodiolae used is the Herba Rhodiolae root; the Herba Angelicae Sinensis used is the Herba Angelicae Sinensis root; and the Herba Coptidis used is the Herba Coptidis Sinensis root.

[0034] Example 1

[0035] The Chinese medicine preparation for improving myocarditis and enhancing immunity in this embodiment is composed of the following raw materials:

[0036] 20g of Rhizoma Niudici, 20g of Yacon, 15g of Astragalus, 20g of Rhodiola, 30g of Agaricus blazei, 15g of Angelica sinensis and 10g of Trichosanthes kirilowii.

[0037] The preparation method of the Chinese medicine preparation is:

[0038] The above raw materials were washed, removed impurities, dried, chopped, and decocted twice. For the first time, 50 vol% ethanol aqueous solution was added in an amount 5 times that of the raw material, decocted for 1.5 h, the decoction was taken, and filtered; for the second time, 6 times the amount of water was added and decocted for 1.5 h, the decoctions were combined, filtered, and dried to obtain the finished product.

[0039] Example 2

[0040] The Chinese medicine preparation for improving myocarditis and enhancing immunity in this embodiment is composed of the following raw materials: 30g of Rhizoma Cynanchum, 15g of Yacon, 15g of Astragalus, 10g of Rhodiola, 40g of Agaricus blazei, 5g of Angelica sinensis and 10g of Rhizoma Cynanchum; the preparation method refers to the method in Example 1.

[0041] Example 3

[0042] 25g of Radix Codonopsis pilosulae, 30g of Yacon, 10g of Radix Astragali, 10g of Rhodiola rosea, 20g of Agaricus blazei, 10g of Angelica sinensis and 20g of Radix Codonopsis pilosulae; the preparation method refers to the method in Example 1.

[0043] Example 4

[0044] Rhizoma Cynanchifolia 10g, Rhodiola rosea 10g, Agaricus blazei 10g, Angelica sinensis 5g and Rhizoma Cynanchum 5g; the preparation method refers to the method in Example 1.

[0045] Example 5

[0046] 30g of Radix Codonopsis pilosulae, 30g of Yacon, 20g of Radix Astragali, 20g of Rhodiola rosea, 50g of Agaricus blazei, 25g of Angelica sinensis and 20g of Radix Codonopsis pilosulae; the preparation method refers to the method in Example 1.

[0047] Comparative Example 1

[0048] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Radix Codonopsis pilosulae, Yacon, Radix Astragali, Rhodiola rosea, Agaricus blazei and Angelica sinensis; the missing amount of Radix Codonopsis pilosulae is distributed to the corresponding components according to the proportion of other substances.

[0049] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0050] Comparative Example 2

[0051] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Radix Glehniae, Yacon, Radix Astragali, Rhodiola Rosea, Agaricus Blazei and Radix Angelicae Pubescentis; the missing amount of Angelica sinensis is distributed to the corresponding components according to the proportion of other substances.

[0052] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0053] Comparative Example 3

[0054] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Radix Glechomae Dahuricae, Yacon, Radix Astragali, Rhodiola Rosea, Radix Angelicae Sinensis and Radix Eupatorii; the amount of the missing Agaricus blazei Murrill is distributed to the corresponding components according to the proportion of other substances.

[0055] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0056] Comparative Example 4

[0057] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Rhizoma Cynomorii, Yacon, Astragalus, Angelica, Agaricus blazei and Rhizoma Cynomorii; the amount of Rhodiola rosea missing is distributed to the corresponding components according to the proportion of other substances.

[0058] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0059] Comparative Example 5

[0060] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Radix Glehniae, Yacon, Rhodiola rosea, Angelica sinensis, Agaricus blazei and Rhizoma Coptidis; the missing amount of Astragalus is distributed to the corresponding components according to the proportion of other substances.

[0061] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0062] Comparative Example 6

[0063] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes Radix Glehniae, Radix Astragali, Radix Rhodiolae, Radix Angelicae Sinensis, Agaricus Blazei and Radix Eupatorii; the missing amount of yacon is distributed to the corresponding components according to the proportion of other substances.

[0064] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0065] Comparative Example 7

[0066] The Chinese medicine preparation of this comparative example is different from that of Example 1 in that this comparative example only includes yacon, astragalus, rhodiola rosea, angelica, Agaricus blazei and Rhizoma Coptidis; the amount of Herba Cynoglossi missing is distributed to the corresponding components according to the proportion of other substances.

[0067] The preparation method of the Chinese medicine preparation is carried out with reference to that in Example 1.

[0068] Test example

[0069] This test example explores the therapeutic effect of the Chinese medicine preparation obtained in the embodiment and the comparative example on mice with myocarditis, and specifically comprises the following steps:

[0070] (1) 120 BALC / c mice were selected and randomly divided into a model group (10 mice), a test group (10 mice in each group of Examples 1 to 3 and Comparative Examples 1 to 7), and a control group (10 mice); wherein, each mouse in the model group and the test group was intraperitoneally injected with 0.1 ml of CNB3 virus solution containing 100 TTCID50 / 0.1 ml of Coxsackievirus B3 (CVB3) for 2 consecutive times to establish a mouse viral myocarditis model; the control group was simultaneously injected with an equal amount of normal saline.

[0071] (2) Two hours after the first injection of the virus solution, the mice were given intragastric administration. The test group was given 5 g / kg of the Chinese medicine preparation obtained in the examples and comparative examples; the model group and the control group were given an equal volume of distilled water at the same time; the drugs were administered once a day for 14 consecutive days.

[0072] (3) The mice were anesthetized and subjected to transthoracic echocardiography using a small animal ultrasound system. The parasternal long-axis and short-axis sections were used to analyze the left ventricular structure and function. The left ventricular end-diastolic diameter (LVEDD), left ventricular posterior wall thickness (LVPWs), and left ventricular anterior wall thickness (LVAWs) were displayed in M ​​mode, and the left ventricular ejection fraction (EF) and left ventricular shortening fraction (FS) were calculated. The experimental results are shown in Table 1.

[0073] Table 1 Left ventricular ejection fraction and left ventricular shortening test results

[0074]

[0075]

[0076] It can be seen from Table 1 that, compared with the comparative example, the Chinese medicine preparation of the embodiment of the present invention can significantly improve the left ventricular ejection fraction and left ventricular shortening rate of mice. The complete Chinese medicine preparation formula has a greater impact on the final test results. If a certain component is missing, the improvement rate of the left ventricular ejection fraction and left ventricular shortening rate of mice will be reduced.

[0077] (4) Serum myocardial enzyme and inflammatory factor detection: After the last administration, fast and drink no water for 12 hours, weigh, draw blood from the ophthalmic artery, centrifuge at 4000r / min for 6 minutes, take the supernatant, and measure the serum creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and inflammatory factor (TNF-α) by ELISA. The test results are shown in Table 2.

[0078] Table 2 Results of serum myocardial enzymes and inflammatory factors

[0079]

[0080]

[0081] It can be seen from Table 2 that, compared with the comparative example, the Chinese medicine preparation of the embodiment of the present invention can significantly reduce the levels of CK-MB, LDH, AST, and TNF-α in mouse serum.

[0082] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A Chinese medicinal preparation for improving myocarditis and enhancing immunity, characterized in that: Calculated by weight, the composition comprises 10-30 parts of Rhizoma Cynoglossi, 15-30 parts of Yacon, 10-20 parts of Radix Astragali, 10-20 parts of Rhodiola rosea, 10-50 parts of Agaricus blazei, 5-25 parts of Angelica sinensis and 5-20 parts of Radix Codonopsis pilosulae.

2. The Chinese medicinal preparation for improving myocarditis and enhancing immunity according to claim 1, characterized in that: The composition comprises, by weight: 20 parts of Radix Glehniae, 20 parts of Yacon, 15 parts of Radix Astragali, 20 parts of Rhodiola Rosea, 30 parts of Agaricus Blazei, 15 parts of Angelica Sinensis and 10 parts of Radix Glehniae; Alternatively, the Chinese medicine preparation for improving myocarditis and enhancing immunity comprises, by weight: 30 parts of Rhizoma Cynomorii, 15 parts of Yacon, 15 parts of Astragalus, 10 parts of Rhodiola rosea, 40 parts of Agaricus blazei, 5 parts of Angelica sinensis and 10 parts of Rhizoma Cynomorii; Alternatively, the Chinese medicinal preparation for improving myocarditis and enhancing immunity comprises, by weight, 25 parts of Rhizoma Cynomorii, 30 parts of Yacon, 10 parts of Astragalus, 10 parts of Rhodiola rosea, 20 parts of Agaricus blazei Murrill, 10 parts of Angelica sinensis and 20 parts of Rhizoma Cynomorii.

3. The Chinese medicinal preparation for improving myocarditis and enhancing immunity according to claim 1, characterized in that: The Herba Cynanchii is the Herba Cynanchii root; the Herba Astragali is the root; the Herba Rhodiolae is the Herba Rhodiolae root; the Herba Angelicae Sinensis is the Herba Angelicae Sinensis root; and the Herba Corydalis is the Herba Corydalis root.

4. The method for preparing the Chinese medicinal preparation for improving myocarditis and enhancing immunity according to any one of claims 1 to 3, characterized in that: include: The method comprises the following steps: crushing 10-30 parts of Radix Codonopsis pilosulae, 15-30 parts of yacon, 10-20 parts of astragalus, 10-20 parts of rhodiola rosea, 10-50 parts of Agaricus blazei, 5-25 parts of angelica sinensis and 3-10 parts of Radix Codonopsis pilosulae, mixing with a 50 vol% ethanol aqueous solution, boiling for 1-2 hours, taking the decoction and filtering; mixing the filter residue with water, boiling for 1-2 hours, combining the decoction and drying to obtain a traditional Chinese medicine preparation for improving myocarditis and enhancing immunity.

5. Use of the Chinese medicinal preparation for improving myocarditis and enhancing immunity according to any one of claims 1 to 3 in the preparation of products for preventing and / or treating myocarditis.

6. The use according to claim 5, characterized in that: The myocarditis includes viral myocarditis.

7. The use according to claim 5, characterized in that: The dosage of the traditional Chinese medicine preparation is 5 to 6 g / kg.

8. Use of the Chinese medicinal preparation for improving myocarditis and enhancing immunity according to any one of claims 1 to 3 in the preparation of a product for improving left ventricular ejection fraction and / or left ventricular shortening rate.

9. Use of the Chinese medicinal preparation for improving myocarditis and enhancing immunity according to any one of claims 1 to 3 in the preparation of one or more products for reducing (1) to (4); (1) Serum creatine kinase isoenzymes; (2) Lactate dehydrogenase; (3) Aspartate aminotransferase; (4) Inflammatory factors.

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