Dental pulp stem cell culture device

Abstract

The invention discloses a dental pulp stem cell culture device, and relates to the technical field of stem cell culture, the dental pulp stem cell culture device comprises a culture box, and square conveying assemblies, a driving assembly, a sliding assembly, a pressing assembly and a culture plate which are located in the culture box, the two square conveying assemblies synchronously rotate through the driving assembly, and a groove is formed in the culture plate; each groove is internally and correspondingly provided with one culture dish, each culture dish comprises a base and a top cover, the top cover is provided with a sliding rail, a sliding cover and a liquid injection hole, the sliding assembly comprises an air cylinder, a support and a connecting piece, the support and the placing barrel slide through the air cylinder, and the pressing assembly comprises a cover plate, a rotating motor and a transmission assembly. The cover plate is connected with the rotating motor through a transmission assembly. The adding efficiency is improved through the square conveying assembly, the pressing assembly and the sliding assembly, nutrient solution adding can be conducted on culture dishes on the multiple culture plates, meanwhile, nutrient solutions with different concentrations can be replaced by replacing injectors, and the adding flexibility is improved.

Description

Technical Field

[0001] The invention relates to the technical field of stem cell culture, in particular to a dental pulp stem cell culture device. Background Art

[0002] Dental pulp stem cells are the only soft tissue in the tooth, located in the pulp cavity inside the tooth. Dental pulp stem cells have the potential for multidirectional differentiation. In addition to being able to form mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelium, liver, nerve and other cell line types after being induced by different cytokines. In the existing culture of dental pulp stem cells, they usually need to be placed in a culture dish for observation and cultivation.

[0003] However, when adding nutrient solution to the existing device, the cover needs to be opened, which makes it difficult to quickly perform the filling operation. In addition, the culture dish is easily contaminated over a long period of time, thereby affecting the culture effect of stem cells.

[0004] When adding nutrient solution to multiple culture dishes at the same time, it is necessary to add the nutrient solution one by one, which is labor-intensive and has low adding efficiency. Summary of the invention

[0005] The object of the present invention is to provide a dental pulp stem cell culture device to solve the problems existing in the background technology.

[0006] The objective of the present invention is achieved through the following technical solutions: A dental pulp stem cell culture device, comprising a culture box and a square conveying assembly, a driving assembly, a sliding assembly, a pressing assembly and a culture plate located in the culture box, wherein two groups of the square conveying assemblies are located on both sides of the interior of the culture box, the driving assembly is located below the square conveying assembly and at the bottom of the culture box, and the two groups of the square conveying assemblies are synchronously rotated by the driving assembly; The culture plate is located between the two square conveying assemblies and is rotatably connected to the two square conveying assemblies respectively. A plurality of grooves are evenly arrayed on the culture plate, and a culture dish is correspondingly arranged in each of the grooves. The culture dish comprises a base and a top cover. The top cover is provided with a slide rail, a slide cover and a liquid injection hole. The liquid injection hole is located at the center of the top cover. The two slide rails are located on both sides of the liquid injection hole. The slide cover is slidably connected with the slide rail and is located above the liquid injection hole. An abutment plate is provided above the slide cover, and a connecting plate is provided on the top cover. A first spring is provided between the abutment plate and the connecting plate. The sliding assembly includes a cylinder, a bracket and a connecting piece, the sliding assembly is located above the square conveying assembly, sliding rods are provided on the front and rear sides of the incubator, and sliding blocks matched with the sliding rods are provided on the front and rear ends of the bracket. The bracket is slidably connected to the incubator through the sliding rods and the sliding blocks. A placement cylinder is provided in the middle of the bracket, and the placement cylinder is located on the side of the abutment plate away from the connecting plate. The cylinder is fixed to one side of the interior of the incubator, and the piston rod of the cylinder is detachably connected to the bracket through a connecting piece. The bracket and the placement cylinder slide through the cylinder, and the sliding cover is opened by the abutment of the placement cylinder. A syringe is arranged in the placing tube, and the inner dimension of the placing tube matches the outer dimension of the syringe; The pressing assembly includes a cover plate, a rotating motor and a transmission assembly. The cover plate is located at the top of the incubator. A mounting plate is provided on the cover plate. A second spring is provided between the mounting plate and the cover plate. A pressure rod is provided below the mounting plate. The pressure rod is located above the injection hole. The pressure rod passes through the cover plate corresponding to the injection hole. The cover plate is connected to the rotating motor through the transmission assembly.

[0007] Further, the transmission assembly includes a sleeve, a spline shaft, a first bevel gear, a second bevel gear, a spur gear and a rack. The motor seat of the rotating motor is fixed on the side wall of the incubator. One end of the sleeve is rotatably connected to the side wall of the incubator. The sleeve is provided with a gear ring. The rotating motor is connected to the sleeve through the gear ring. One end of the spline shaft is inserted into the sleeve and is slidably connected to the sleeve. The first bevel gear is located at the other end of the spline shaft. A bearing is also provided on the spline shaft. The inner ring of the bearing is tightly matched with the spline shaft. The outer ring of the bearing is connected to the connecting piece. One end of the rack is fixed to the mounting plate, and the other end of the rack passes through the cover plate. The spur gear and the second bevel gear are located below the top cover. The spur gear is meshed with the rack. Two groups of the spur gears and the rack are respectively located on both sides of the second bevel gear, and the second bevel gear is meshed with the first bevel gear.

[0008] Furthermore, a displacement sensor is provided below the cover plate on one side of the pressure rod, and the displacement sensor corresponds to the top of the syringe.

[0009] Furthermore, the square conveying assembly includes a chain belt and a transmission wheel, one end of the transmission wheel is connected to the side wall of the incubator through a rotating shaft, a connecting rod is provided on the chain belt, fixed blocks are provided on both sides of the culture plate, the connecting rod is inserted into the fixed block and rotatably connected to the fixed block, and the fixed block is provided with a socket above the connecting rod.

[0010] Furthermore, the connecting member includes a vertical rod, a fixed rod and an insertion rod, one side of the vertical rod is fixed to the piston rod of the cylinder, the top of the vertical rod is fixed to the outer ring of the bearing, the fixed rod is located on the side of the vertical rod away from the cylinder, the end of the fixed rod away from the cylinder is connected to the cylinder by bolts, the length of the fixed rod is greater than the insertion rod, the insertion rod is located below the fixed rod, and the insertion rod corresponds to the socket.

[0011] Furthermore, a limit block is provided on one side of the top cover, a slot is provided at the position of the groove corresponding to the limit block, grabbing grooves are provided on both sides of the groove, the limit block and the first spring are on the same axis, and the axial direction of the limit block and the first spring is parallel to the axial direction of the cylinder.

[0012] Furthermore, the driving assembly includes a driving motor and a reversing assembly, the driving motor is fixed at the bottom of the incubator, two reversing assemblies are located on both sides of the driving motor, and the reversing assembly is connected to the square conveying assembly.

[0013] Furthermore, a binocular camera is provided below the cover plate.

[0014] Furthermore, a detachable front panel is provided on the front of the incubator, and the front panel, cover plate and mounting plate are made of transparent plastic material.

[0015] The beneficial effects of the present invention are: 1) There are multiple culture plates in the incubator, and each culture plate is equipped with multiple culture dishes, which can culture multiple compressed stem cells and increase the number of culture dishes. 2) Nutrient solution can be added to all culture dishes on a culture plate at one time through the pressing component and the sliding component, which greatly improves the addition efficiency.

[0016] 3) By combining the square conveying components, nutrient solution can be added to the culture dishes on multiple culture plates.

[0017] 4) You can change the nutrient solution of different concentrations by replacing the syringe, which increases the flexibility of addition.

[0018] 5) The entire process of adding nutrient solution takes place in the incubator, which greatly reduces external pollution and ensures the culture effect of dental pulp stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a front view of the dental pulp stem cell culture device of the present invention when the cylinder is not started; Figure 2 A top view of the dental pulp stem cell culture device of the present invention when the cylinder is not activated; Figure 3 for Figure 1 The enlarged schematic diagram of point A in the middle; Figure 4 for Figure 2 The enlarged schematic diagram of point B in the middle; Figure 5 It is a front view of the dental pulp stem cell culture device of the present invention when the cylinder is activated; Figure 6 for Figure 5 The enlarged schematic diagram of the center C; Figure 7 It is a side view of the square conveying assembly of the present invention; Figure 8 is a top view of the groove in the present invention; Fig. 9 is a side view of the culture dish of the present invention; Fig.10 is a top view of the bracket in the present invention; Fig.11 is a schematic diagram of a connecting member in the present invention; Fig.12 It is a schematic diagram of the installation of placing the barrel and the syringe in the present invention; Fig.13 A bottom view of the pressing assembly in the present invention; In the figure, 1-incubator, 2-square conveying assembly, 21-connecting rod, 22-chain belt, 23-transmission wheel, 3-driving assembly, 31-driving motor, 32-reversing assembly, 4-culture plate, 41-groove, 42-fixed block, 43-grasping groove, 44-jack, 5-sliding assembly, 51-sliding rod, 52-sliding block, 53 placing cylinder, 54-vertical rod, 55-fixed rod, 56-insertion rod, 57-cylinder, 58-bracket, 6-pressing assembly , 61-mounting plate, 62-pressure rod, 63-displacement sensor, 64-rack, 65-spur gear, 66-second bevel gear, 67-first bevel gear, 68-bearing, 69-spline shaft, 610-sleeve, 611-rotating motor, 612-cover plate, 613-second spring, 7-culture dish, 71-base, 72-top cover, 73-slide cover, 74-slide rail, 75-abutment plate, 76-connecting plate, 77-first spring, 8-syringe. DETAILED DESCRIPTION

[0020] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0021] See also Figure 1-Figure 13 , the present invention provides a technical solution: like Figure 1-Figure 13 As shown, the dental pulp stem cell culture device includes a culture box 1 and a square conveying component 2, a driving component 3, a sliding component 5, a pressing component 6 and a culture plate 4 located in the culture box 1.

[0022] The two groups of the square conveying components 2 are located on both sides of the interior of the incubator 1 , the driving component 3 is located below the square conveying components 2 and at the bottom of the incubator 1 , and the two groups of the square conveying components 2 rotate synchronously through the driving component 3 .

[0023] Specifically, the square conveying assembly 2 includes a chain belt 22 and a transmission wheel 23, one end of the transmission wheel 23 is connected to the side wall of the incubator 1 through a rotating shaft, the driving assembly 3 includes a driving motor 31 and a reversing assembly 32, the driving motor 31 is fixed to the bottom of the incubator 1, the two reversing assemblies 32 are located on both sides of the driving motor 31, and the reversing assembly 32 is connected to the square conveying assembly 2.

[0024] Through the above technical scheme, the driving motor 31 is connected to the two square conveying components 2 through the reversing component 32, and the two square conveying components 2 are driven to rotate synchronously by the driving motor 31. At the same time, the square conveying component 2 in this embodiment adopts the structure of a chain belt 22 and a rotating wheel to ensure that the chain belt 22 transmits smoothly without slipping, while improving the supporting effect of the culture plate 4.

[0025] Furthermore, the culture plate 4 is located between the two square conveying components 2 and is rotatably connected to the two square conveying components 2 respectively. A connecting rod 21 is provided on the chain belt 22. Fixed blocks 42 are provided on both sides of the culture plate 4. The connecting rod 21 is inserted into the fixed block 42 and is rotatably connected to the fixed block 42.

[0026] Through the above technical solution, and as Figure 1 and Figure 7 As shown, a plurality of culture plates 4 are arranged on the chain belt 22. When the chain belt 22 is continuously rotated by the driving motor 31, the culture plates 4 will follow the chain belt 22 to cyclically rotate. Since the fixing block 42 on the culture plate 4 is rotatably connected with the connecting rod 21 on the chain belt 22, the culture plate 4 can be quickly reset to keep level under the action of its own weight, and can always be in a horizontal state when the chain belt 22 rotates, ensuring that the culture dish 7 on the culture plate 4 will not tilt.

[0027] Furthermore, the culture plate 4 is evenly arrayed with a plurality of grooves 41, and a culture dish 7 is correspondingly arranged in each of the grooves 41. The culture dish 7 includes a base 71 and a top cover 72. The top cover 72 is provided with a slide rail 74, a slide cover 73 and an injection hole. The injection hole is located at the center of the top cover 72, and the two slide rails 74 are located on both sides of the injection hole. The slide cover 73 is slidably connected to the slide rail 74 and is located above the injection hole. An abutment plate 75 is provided above the slide cover 73, and a connecting plate 76 is provided on the top cover 72. A first spring 77 is provided between the abutment plate 75 and the connecting plate 76.

[0028] Through the above technical solution, and as Figure 5 , Figure 6 , Figure 8 and Fig. 9 As shown, the culture dish 7 is placed on the culture plate 4. One culture plate 4 can hold multiple culture dishes 7, so that a large number of dental pulp stem cells can be cultured. Grasping grooves 43 are provided on both sides of the groove 41 to facilitate placing or removing the culture dish 7 from the groove 41.

[0029] The top cover 72 and the base 71 of the culture dish 7 just fit into the groove 41. An opening and closing structure is provided on the top cover 72. When the first spring 77 is in the reset state, the sliding cover 73 is located above the injection hole to cover the injection hole to prevent the inside of the culture dish 7 from contacting the outside world. When the abutment plate 75 on the sliding cover 73 is pushed to move toward the connecting plate 76, the sliding cover 73 slides along the slide rail 74 and compresses the first spring 77 at the same time. The sliding cover 73 opens to expose the injection hole, and nutrient solution can be added to the culture dish 7 from the injection hole at this time; when the pressure on the abutment plate 75 is cancelled, the sliding cover 73 is reset under the action of the first spring 77, and the injection hole is blocked.

[0030] At the same time, a limit block is provided on one side of the top cover 72, and the groove 41 is provided with a slot corresponding to the position of the limit block. The culture dish 7 can be placed into the groove 41 in a specified direction through the limit block, so that the subsequent sliding component 5 can open the sliding cover 73.

[0031] Furthermore, if Figure 5 , Fig.10 , Fig.11 As shown, the sliding assembly 5 includes a cylinder 57, a bracket 58 and a connecting piece. The sliding assembly 5 is located above the square conveying assembly 2. Sliding rods 51 are provided on the front and rear sides of the incubator 1. Sliding blocks 52 that cooperate with the sliding rods 51 are provided on the front and rear ends of the bracket 58. The bracket 58 is slidably connected to the incubator 1 through the sliding rods 51 and the sliding blocks 52.

[0032] At the same time, a placement tube 53 is provided in the middle of the bracket 58, and a placement tube 53 is correspondingly provided on one side of each culture dish 7. At the same time, the placement tube 53 is located on the side of the abutment plate 75 away from the connecting plate 76. The cylinder 57 is fixed on one side of the incubator 1, and the piston rod of the cylinder 57 is detachably connected to the bracket 58 through a connecting piece.

[0033] According to the above technical solution, when the cylinder 57 drives the bracket 58 to move through the connecting piece, the bracket 58 slides along the slide bar 51 through the slider 52, and the placement tube 53 on the bracket 58 moves synchronously, and the placement tube 53 contacts the abutment plate 75, and the placement tube 53 drives the abutment plate 75 to move and then open the slide cover 73. When the slide cover 73 abuts the connecting plate 76, the injection hole is completely exposed, and the center of the placement tube 53 is just aligned with the center of the injection hole.

[0034] Furthermore, the connecting member includes a vertical rod 54, a fixed rod 55 and an insertion rod 56, one side of the vertical rod 54 is fixed to the piston rod of the cylinder 57, the top of the vertical rod 54 is fixed to the outer ring of the bearing 68, the fixed rod 55 is located on the side of the vertical rod 54 away from the cylinder 57, the end of the fixed rod 55 away from the cylinder 57 is connected to the cylinder 57 by bolts, the length of the fixed rod 55 is greater than the insertion rod 56, the insertion rod 56 is located below the fixed rod 55, and the insertion rod 56 corresponds to the socket 44.

[0035] Through the above technical solution, the vertical rod 54 is connected to the piston rod of the cylinder 57, and the fixed rod 55 is detachably connected to the middle part of the bracket 58. When the cylinder 57 is not started, Figure 1 and Figure 2 As shown, at this time, the cylinder 57 is located on the right side of the incubator 1 , and the placement tube 53 is located on the right side of the abutment plate 75 , which does not affect the cyclic rotation of the culture plate 4 following the chain belt 22 .

[0036] like Figure 5 and Figure 6 As shown, when a culture plate 4 just moves to the top of the square conveying assembly 2, the chain belt 22 stops rotating and the cylinder 57 starts. The cylinder 57 pushes the bracket 58 to move leftward through the fixing rod 55, and the insertion rod 56 is inserted into the insertion hole 44 of the fixing block 42. Since the insertion rod 56 cooperates with the insertion hole 44, the insertion rod 56 is used to limit the position, and the culture plate 4 will not rotate at this time, so that the shaking of the culture plate 4 is avoided to affect the culture dish 7. As the cylinder 57 works, the slide cover 73 is fully opened until the placement tube 53 is placed.

[0037] Furthermore, if Figure 6 and Fig.12As shown, the syringe 8 is placed in the placement tube 53, and the inner size of the placement tube 53 matches the outer size of the syringe 8. The syringe 8 is used to extract the nutrient solution in advance. When the placement tube 53 opens the slide cover 73, the syringe 8 is located in the placement tube 53 and aligned with the injection hole. Under the action of the subsequent pressing component 6, the syringe 8 will inject the nutrient solution into the culture dish 7.

[0038] Furthermore, if Figure 6 and Fig.13 As shown, the pressing assembly 6 includes a cover plate 612, a rotating motor 611 and a transmission assembly. The cover plate 612 is located at the top of the incubator 1. A mounting plate 61 is provided on the cover plate 612. A second spring 613 is provided between the mounting plate 61 and the cover plate 612. A pressure rod 62 is provided below the mounting plate 61. The pressure rod 62 is correspondingly located above the injection hole. The pressure rod 62 passes through the cover plate 612 corresponding to the injection hole. The cover plate 612 is connected to the rotating motor 611 through a transmission assembly. The transmission assembly includes a sleeve 610, a spline shaft 69, a first bevel gear 67, a second bevel gear 66, a spur gear 65 and a rack 64. The motor seat of the rotating motor 611 is fixed on the side wall of the incubator 1. One end of the sleeve 610 is rotatably connected to the side wall of the incubator 1. The sleeve 610 A gear ring is provided on it, and the rotating motor 611 is connected to the sleeve 610 through the gear ring. One end of the spline shaft 69 is inserted into the sleeve 610 and is slidably connected to the sleeve 610. The first bevel gear 67 is located at the other end of the spline shaft 69. A bearing 68 is also provided on the spline shaft 69. The inner ring of the bearing 68 is tightly matched with the spline shaft 69, and the outer ring of the bearing 68 is connected to the connecting piece. One end of the rack 64 is fixed on the mounting plate 61, and the other end of the rack 64 passes through the cover plate 612. The spur gear 65 and the second bevel gear 66 are located below the top cover 72, and the spur gear 65 is meshed with the rack 64. Two groups of the spur gears 65 and the rack 64 are respectively located on both sides of the second bevel gear 66, and the second bevel gear 66 is meshed with the first bevel gear 67.

[0039] Through the above technical solution, the cover plate 612 is detachably connected to the incubator 1, and the two racks 64 are located on both sides of the placement tube 53 and will not affect the movement of the bracket 58. The mounting frame and the cover plate 612 bracket 58 are supported by the second spring 613. When the cylinder 57 moves, since the vertical rod 54 is connected to the outer ring of the bearing 68, the spline shaft 69 will be pushed out of the sleeve 610. At this time, the first bevel gear 67 moves to the left and meshes with the second bevel gear 66. When the rotating motor 611 is started, the rotating motor 611 drives the sleeve 610 to rotate. Since the sleeve 610 is key-connected with the spline shaft 69, the spline shaft 69 drives the first bevel gear 67 to rotate, and then through the second bevel gear 66 and the spur gear 65, the lifting and lowering of the rack 64 is realized. Since the rack 64 is connected to the mounting plate 61, the pressure rod 62 is located below the mounting plate 61, so the lifting and lowering of the pressure rod 62 is finally realized. Since the pressure rod 62 is located above the injection hole, when the syringe 8 is located above the injection hole through the cylinder 57, as the pressure rod 62 descends, the nutrient liquid in the syringe 8 can be pressed out to complete the addition to the culture dish 7.

[0040] Furthermore, a displacement sensor 63 is provided below the cover plate 612 and on one side of the pressure rod 62 , and the displacement sensor 63 corresponds to the top of the syringe 8 .

[0041] Through the above technical solution, the distance between the end of the piston shaft of the syringe 8 and the displacement sensor 63 can be obtained through the displacement sensor 63. Since the diameter of the syringe 8 is determined, the volume is the product of the bottom area and the height. That is, the volume of the pressed nutrient solution can be determined by judging the distance the piston shaft of the syringe 8 is pressed, thereby achieving quantitative addition to the culture dish 7.

[0042] Furthermore, a binocular camera is provided below the cover plate 612 .

[0043] Through the above technical solution, the position of the culture plate 4 is identified by the binocular camera. When the culture plate 4 is rotated to the top through the chain belt 22, the drive motor 31 stops, and the cylinder 57 and the rotating motor 611 are started to complete the addition of the nutrient solution.

[0044] Furthermore, a detachable front panel is provided on the front of the incubator 1 , and the front panel, the cover plate 612 and the mounting plate 61 are made of transparent plastic material.

[0045] Through the above technical solution, the culture dish 7 can be taken out or put in by opening the front panel. As the chain belt 22 rotates slowly, the culture dish 7 can be put in and taken out continuously, and the panel of transparent plastic material can be easily observed.

[0046] Specific implementation method, such as Figure 1-Figure 4As shown, the driving motor 31 and the cylinder 57 are not started, and the culture dish 7 is cultured statically in the incubator 1. A heating and humidifying component can be provided in the incubator 1 as required.

[0047] When it is necessary to add nutrient solution to the culture dish 7, since the cylinder 57 is not started, the cover 612 is opened and the bracket 58 is placed on the slide rod 51. Since the vertical rod 54 is also detachably connected to the piston rod of the cylinder 57, the connecting parts can be connected to the bracket 58 and the cylinder 57 respectively. At the same time, multiple syringes 8 with fixed volumes of nutrient solution extracted in advance are placed in multiple placement tubes 53, and the cover 612 is reset.

[0048] like Figure 5-6 As shown, the drive motor 31 is started at this time, and as the chain belt 22 rotates, the culture plate 4 rotates accordingly. When the binocular camera determines that a culture plate 4 is directly above, the drive motor 31 is stopped and the cylinder 57 is started.

[0049] The cylinder 57 pushes the bracket 58 to move leftward through the fixed rod 55, and the plug rod 56 is inserted into the insertion hole 44 of the fixed block 42. Since the plug rod 56 cooperates with the insertion hole 44 and is limited by the plug rod 56, the culture plate 4 will not rotate at this time, thereby preventing the culture plate 4 from shaking and affecting the culture dish 7. As the cylinder 57 continues to push, the bracket 58 slides along the slide rod 51 through the slider 52, and the placement tube 53 on the bracket 58 moves synchronously. The placement tube 53 contacts the abutment plate 75, and the placement tube 53 drives the abutment plate 75 to move and then open the slide cover 73. When the slide cover 73 abuts the connecting plate 76, the injection hole is completely exposed, and the center of the placement tube 53 is just aligned with the center of the injection hole.

[0050] As the cylinder 57 moves, the first bevel gear 67 moves to the left and meshes with the second bevel gear 66. When the rotating motor 611 is started, the pressure rod 62 is finally lowered under the action of the second bevel gear 66, the spur gear 65 and the rack 64, and quantitative liquid addition is achieved through the displacement sensor 63. At the same time, the nutrient solution can be added to all the culture dishes 7 on a culture plate 4 at one time through the pressing component 6 and the sliding component 5, which greatly improves the addition efficiency.

[0051] When the culture dish 7 on a culture plate 4 is added, the cylinder 57 is reset, the drive motor 31 is started, and the next culture plate 4 is moved to the top through the chain belt 22 for the next round of addition. This solution can realize the automatic addition of multiple culture dishes 7, freeing up manpower and improving the addition efficiency. At the same time, different concentrations of nutrient solution can be replaced by replacing the syringe 8 to improve the flexibility of addition. At the same time, the entire process of addition is located in the incubator 1, which greatly reduces external pollution and ensures the culture effect of dental pulp stem cells.

[0052] The above is only a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the concept described herein through the above teachings or the technology or knowledge of the relevant field. The changes and modifications made by those skilled in the art do not depart from the spirit and scope of the present invention, and should be within the scope of protection of the claims attached to the present invention.

Claims

1. A dental pulp stem cell culture device, characterized in that: The invention comprises an incubator (1) and a square conveying assembly (2), a driving assembly (3), a sliding assembly (5), a pressing assembly (6) and a culture plate (4) located inside the incubator (1), wherein two groups of the square conveying assemblies (2) are located on both sides of the interior of the incubator (1), the driving assembly (3) is located below the square conveying assemblies (2) and at the bottom of the incubator (1), and the two groups of the square conveying assemblies (2) are rotated synchronously by the driving assembly (3); The culture plate (4) is located between the two square conveying assemblies (2) and is rotatably connected to the two square conveying assemblies (2) respectively. The culture plate (4) is evenly arrayed with a plurality of grooves (41), and a culture dish (7) is correspondingly arranged in each groove (41). The culture dish (7) comprises a base (71) and a top cover (72). The top cover (72) is provided with a slide rail (74), a slide cover (73) and a liquid injection hole. The liquid injection hole is located at the center of the top cover (72). The two slide rails (74) are located on both sides of the liquid injection hole. The slide cover (73) is slidably connected to the slide rail (74) and is located above the liquid injection hole. An abutment plate (75) is provided above the slide cover (73). A connecting plate (76) is provided on the top cover (72). A first spring (77) is provided between the abutment plate (75) and the connecting plate (76). The sliding assembly (5) comprises a cylinder (57), a bracket (58) and a connecting piece. The sliding assembly (5) is located above the square conveying assembly (2). The front and rear sides of the incubator (1) are provided with sliding rods (51). The front and rear ends of the bracket (58) are provided with sliding blocks (52) that cooperate with the sliding rods (51). The bracket (58) is slidably connected to the incubator (1) through the sliding rods (51) and the sliding blocks (52). A placement tube (53) is provided in the middle of the bracket (58). The placement tube (53) is located on a side of the abutment plate (75) away from the connection plate (76). The cylinder (57) is fixed to an inner side of the incubator (1). The piston rod of the cylinder (57) is detachably connected to the bracket (58) through the connecting piece. The bracket (58) and the placement tube (53) slide through the cylinder (57). The sliding cover (73) is opened by the abutment of the placement tube (53). A syringe (8) is arranged in the placement tube (53), and the inner dimensions of the placement tube (53) match the outer dimensions of the syringe (8); The pressing assembly (6) comprises a cover plate (612), a rotating motor (611) and a transmission assembly. The cover plate (612) is located at the top of the incubator (1). A mounting plate (61) is provided on the cover plate (612). A second spring (613) is provided between the mounting plate (61) and the cover plate (612). A pressing rod (62) is provided below the mounting plate (61). The pressing rod (62) is located above the injection hole. The pressing rod (62) passes through the cover plate (612) corresponding to the injection hole. The cover plate (612) is connected to the rotating motor (611) via the transmission assembly.

2. The dental pulp stem cell culture device according to claim 1, characterized in that: The transmission assembly comprises a sleeve (610), a spline shaft (69), a first bevel gear (67), a second bevel gear (66), a spur gear (65) and a rack (64); a motor base of the rotating motor (611) is fixed on the side wall of the incubator (1); one end of the sleeve (610) is rotatably connected to the side wall of the incubator (1); a gear ring is provided on the sleeve (610); the rotating motor (611) is connected to the sleeve (610) via the gear ring; one end of the spline shaft (69) is inserted into the sleeve (610) and is slidably connected to the sleeve (610); the first bevel gear (67) is located at the other end of the spline shaft (69); A bearing (68) is also provided on the spline shaft (69), the inner ring of the bearing (68) is tightly fitted with the spline shaft (69), the outer ring of the bearing (68) is connected to the connecting piece, one end of the rack (64) is fixed on the mounting plate (61), the other end of the rack (64) passes through the cover plate (612), the spur gear (65) and the second bevel gear (66) are located below the top cover (72), the spur gear (65) is meshed with the rack (64), the two groups of the spur gear (65) and the rack (64) are respectively located on both sides of the second bevel gear (66), and the second bevel gear (66) is meshed with the first bevel gear (67).

3. The dental pulp stem cell culture device according to claim 1, characterized in that: A displacement sensor (63) is provided below the cover plate (612) on one side of the pressure rod (62), and the displacement sensor (63) corresponds to the top of the syringe (8).

4. The dental pulp stem cell culture device according to claim 2, characterized in that: The square conveying assembly (2) comprises a chain belt (22) and a transmission wheel (23), one end of the transmission wheel (23) is connected to the side wall of the incubator (1) via a rotating shaft, a connecting rod (21) is provided on the chain belt (22), and fixed blocks (42) are provided on both sides of the culture plate (4), the connecting rod (21) is inserted into the fixed block (42) and is rotatably connected to the fixed block (42), and the fixed block (42) is provided with a plug hole (44) above the connecting rod (21).

5. The dental pulp stem cell culture device according to claim 4, characterized in that: The connecting member comprises a vertical rod (54), a fixing rod (55) and an insertion rod (56); one side of the vertical rod (54) is fixed to the piston rod of the cylinder (57); the top of the vertical rod (54) is fixed to the outer ring of the bearing (68); the fixing rod (55) is located on a side of the vertical rod (54) away from the cylinder (57); one end of the fixing rod (55) away from the cylinder (57) is connected to the cylinder (57) by a bolt; the length of the fixing rod (55) is greater than that of the insertion rod (56); the insertion rod (56) is located below the fixing rod (55); and the insertion rod (56) corresponds to the insertion hole (44).

6. The dental pulp stem cell culture device according to claim 1, characterized in that: A limit block is provided on one side of the top cover (72); a slot is provided in the groove (41) at a position corresponding to the limit block; gripping slots (43) are provided on both sides of the groove (41); the limit block and the first spring (77) are located on the same axis; and the axis directions of the limit block and the first spring (77) are parallel to the axis direction of the cylinder (57).

7. The dental pulp stem cell culture device according to claim 1, characterized in that: The driving assembly (3) comprises a driving motor (31) and a reversing assembly (32); the driving motor (31) is fixed to the bottom of the incubator (1); two reversing assemblies (32) are located on both sides of the driving motor (31); and the reversing assembly (32) is connected to the square conveying assembly (2).

8. The dental pulp stem cell culture device according to claim 1, characterized in that: A binocular camera is provided below the cover plate (612).

9. The dental pulp stem cell culture device according to claim 1, characterized in that: The front of the incubator (1) is provided with a detachable front panel, and the front panel, the cover plate (612) and the mounting plate (61) are made of transparent plastic material.

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