Polypeptide extract and preparation method thereof by utilizing beer wet grains
By removing water-soluble impurities and deeply purifying in the process of preparing polypeptides in beer wet granules, the problems of complex processes and low purity are solved, and the preparation of high-purity peptides is achieved, which improves economic benefits and environmental friendliness.
Patent Information
- Application Number
- CN202411536053.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-31
- Publication Date
- 2025-01-03
AI Technical Summary
The existing process flow for preparing peptides for beer wet granules is complex, and the purity of the finished peptide products is low, making it difficult to design a simple, practical and efficient process route to produce high-purity peptide products.
Deep purification, including cleaning, enzymatic treatment, filtration, degreasing, freezing ultrafiltration and drying, is carried out to produce high purity by thoroughly removing water-soluble impurities before enzymatic reaction and avoiding the production of water-soluble impurities during subsequent treatments. peptide finished product.
It realizes simple and practical preparation of high-purity peptide finished products, improves economic benefits, and is environmentally friendly through a process without solid waste emissions, while enriching the use of products.
Smart Images
Figure CN119242749A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of resource utilization of beer wet grains, and in particular relates to a method for preparing polypeptides by utilizing beer wet grains and a polypeptide extract prepared by the method. Background Art
[0002] Brewer's spent grains are the most important by-product of breweries. Every 1,000 liters of beer produced produces 250 kg of wet spent grains. Wet beer spent grains are rich in protein, amino acids and trace elements. Currently, they are mostly used as raw materials for microbial fermentation and for making roughage for ruminants, with little economic benefit.
[0003] Due to the extremely high nutritional value and powerful skin care and beauty effects of peptide products, they are widely used in cosmetics, medicine and food, becoming a high value-added product; and beer grains contain barley protein, malt protein and yeast protein. After the amino acid structures of the three proteins complement each other, their nutritional value is almost perfect. In order to effectively utilize beer grains, it is considered to use beer grains for the preparation of peptide products.
[0004] However, the existing process of preparing peptides from brewer's grains is complicated and the purity of the peptide products is low. How to design a simple, practical and effective process route that can produce high-purity peptide products remains a technical problem that needs to be solved urgently. Summary of the invention
[0005] According to one aspect of the present invention, a method for preparing polypeptides using beer wet grains is provided. The process route is simple, practical and effective. Water-soluble impurities are completely removed before the enzymatic hydrolysis reaction, and any steps that may produce water-soluble impurities are avoided in the subsequent processing process, and the enzymatic hydrolysis products are deeply purified, thereby producing a high-purity peptide product.
[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows:
[0007] A method for preparing polypeptides using beer wet grains, comprising the steps of:
[0008] S10: Clean the beer lees to remove all sugar and other water-soluble substances in the lees;
[0009] S20: enzymatic treatment of beer wet grains to complete the conversion of proteins into peptides;
[0010] S30: filtering, defatting and freezing ultrafiltration of the enzymatic hydrolysis product in sequence to obtain a refined peptide solution;
[0011] S40: The refined peptide solution is concentrated and dried to obtain a peptide product.
[0012] In some technical solutions, in step S30, the following steps are included:
[0013] S31: inputting the enzymatic hydrolysis product into a plate and frame filter press for filtration to obtain a clear liquid after removing the residue;
[0014] S32: the clear liquid after the residue is removed is input into a centrifuge to separate and remove the light fat to obtain a defatted liquid;
[0015] S33: Freeze the defatted liquid to 1-4°C to precipitate a small amount of starch, dextrin and fiber fine particles therein, and then use an ultrafiltration membrane to remove impurities to obtain a refined peptide solution.
[0016] In some technical solutions, the step S30 further includes:
[0017] S34: The residue filtered and centrifuged during step S31 to step S33 is dried and crushed and used as a raw material for bio-leather or for the manufacture of compound feed.
[0018] In some technical solutions, in step S40, the following steps are included:
[0019] S41: inputting the peptide solution into a reduced pressure evaporator to remove most of the water, thereby obtaining a peptide concentrate having a dry matter content of 50%;
[0020] S42: spray-drying the peptide concentrate to obtain a peptide product for food; and / or freeze-drying the peptide concentrate to obtain a peptide product for cosmetics or medicine.
[0021] In some technical solutions, the inlet air temperature of the spray drying process is 200-230°C, and the outlet air temperature is 70-90°C;
[0022] The drying pressure of the freeze-drying process is 0.092-0.096Mpa.
[0023] In some technical solutions, in step S10:
[0024] Use 2-4 times the amount of clean water as the beer wet grains to wash it 2-5 times.
[0025] In some technical solutions, step S20 is specifically:
[0026] Protease is added to the aqueous solution of beer grains to carry out enzymatic reaction. After the enzymatic reaction is completed, the enzyme is quickly inactivated at a relatively low temperature to avoid dextrinization and saccharification of starch.
[0027] In some technical solutions, the protease is a neutral protease, and the addition ratio of the neutral protease is 0.1-0.5%; and / or the temperature of the enzymatic reaction is 40-55°C, the pH value is 7.2, and the reaction time is 5-8 hours; and / or the temperature during enzyme inactivation is 80°C, and the treatment time is within 10 minutes.
[0028] According to another aspect of the present invention, the present invention further provides a polypeptide extract, which is obtained by adopting the above method for preparing polypeptides using beer wet grains, and the mass of peptides in the polypeptide extract is between 85-95%; the application fields of the polypeptide extract include but are not limited to cosmetics, medicine and food fields.
[0029] The present invention adopts the above technical solution to have at least the following beneficial effects:
[0030] 1. The present invention fully cleans the beer wet grains before the enzymatic hydrolysis reaction to remove all sugars and other water-soluble substances in the wet grains, and avoids ineffective fermentation steps that are prone to produce other complex components in the subsequent processing process. In the process of separating and purifying polypeptides, low-temperature ultrafiltration is used to deeply purify the peptide solution, thereby obtaining a high-purity peptide product. This process is simple, practical and effective, and can achieve higher economic benefits;
[0031] 2. In the process of separating and purifying the polypeptide, the residue filtered and centrifuged during the process is dried and crushed and used as a raw material for biological leather or as feed. The whole process has no solid waste discharge, is environmentally friendly, and improves economic benefits;
[0032] 3. The peptide quality of the high-purity peptide product prepared by the present invention is 85-95%. The peptide concentrate is spray-dried to obtain a peptide product for food, and the peptide concentrate is freeze-dried to obtain a peptide product for cosmetics and medicine, which enriches the product usage and further improves economic benefits.
[0033] 4. The present invention uses fully cleaned beer grains for enzymatic hydrolysis to prepare polypeptides, avoiding the pretreatment steps of drying and high-temperature sterilization of the beer grains, and avoiding the loss of effective ingredients due to the inability to hydrolyze the protein into polypeptides due to coagulation; at the same time, it also avoids the use of ethanol for extraction in the pretreatment step, avoids the enzyme in the enzymatic hydrolysis reaction from losing activity due to ethanol, avoids the production workshop from producing flammable and explosive materials, and avoids the loss of peptides in non-ethanol-soluble proteins. BRIEF DESCRIPTION OF THE DRAWINGS
[0034] Figure 1 This is a process flow chart for preparing polypeptides using beer wet grains according to an embodiment of the present invention;
[0035] Figure 2 Schematic diagram of the washing effect in step S10 according to an embodiment of the present invention. DETAILED DESCRIPTION
[0036] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope limited by the appended claims of the present application. The various commonly used reagents used in the examples are all commercially available products.
[0037] Example 1
[0038] See also Figure 1 , provides a method for preparing polypeptides using beer wet grains, comprising the following steps:
[0039] S10: The beer wet grains from the brewery enter the cleaning tank for cleaning to remove all sugars and other water-soluble substances in the wet grains;
[0040] In the above implementation steps, the beer wet grains include fresh wet grains and dried beer wet grains.
[0041] By the attached Figure 2 It can be seen that the dry matter content in the beer wet grains cleaning liquid gradually decreases and tends to be stable with the increasing number of cleaning times. Considering the cost and effect of water washing, it is preferred to use 2-4 times the amount of clean water as the beer wet grains for 2-5 cleanings, which can significantly save energy and ensure the water washing effect.
[0042] The present application completely removes sugar substances during the pretreatment process of beer wet grains, avoids separation and purification in subsequent processes, significantly optimizes the process, makes the preparation process reliable, and reduces production costs.
[0043] The sufficient cleaning of the beer wet grains in the present application is a key step in improving the purity of the peptides. In addition, the pretreatment steps of drying and high-temperature sterilization of the beer wet grains are avoided, and the loss of effective ingredients due to the inability to hydrolyze the protein into polypeptides due to coagulation is avoided; at the same time, ethanol extraction is also avoided in the pretreatment step, and the enzyme in the enzymatic hydrolysis reaction is prevented from losing its activity due to ethanol, and the production of flammable and explosive materials in the production workshop is avoided, and the loss of peptides in non-ethanol soluble proteins is avoided.
[0044] S20: After being fully cleaned, the beer wet grains are input into a reaction tank, and a proper amount of water is added, and 0.1-0.5% by weight of neutral protease is added for stirring, and the reaction is carried out at a temperature of 40-55°C and a pH value of 7.2 for 5-8 hours, and then heated to 80°C, and the enzyme is inactivated within 10 minutes to complete the conversion of protein to polypeptide;
[0045] In this step, the protein in the beer wet grains is insoluble in water like other ingredients. Because a small amount of soluble protein in barley has entered the beer during the brewing process, the washed beer wet grains can be directly input into the reaction tank for enzymatic hydrolysis. Since the protease can only break down protein into peptides and has no effect on other substances, the final product is composed of peptides and other ingredients (wheat bran, starch, fat, a small amount of remaining insoluble protein that has not been completely reacted, etc.), thus completing the production of peptides.
[0046] The present application uses only protease reaction without using other enzymes or fermentation, so as to avoid the production of any other water-soluble substances, thereby reducing the purity of the peptide and making it difficult to purify the peptide.
[0047] The beer wet grains thoroughly cleaned in the present application do not need sterilization treatment before the enzyme reaction. After repeated experiments, it has been verified that the bacteria in the beer wet grains after cleaning are washed away and will not reproduce during the several hours of the enzyme reaction because the enzyme reaction temperature is not within the bacterial reproduction temperature range. High temperature sterilization will instead cause the starch in the beer wet grains to become dextrin and saccharify into sugar and dissolve into the product, causing the purity of the peptide to decrease. When the enzyme is finally inactivated, the temperature is relatively low and the time is short, so the starch therein will not be dextrinized or saccharified.
[0048] S30: filtering, defatting and freezing ultrafiltration of the enzymatic hydrolysis product in sequence to obtain a refined peptide solution;
[0049] Specifically include:
[0050] S31: inputting the enzymatic hydrolysis product into a plate and frame filter press for filtration to obtain a clear liquid after removing the residue;
[0051] S32: the clear liquid after the residue is removed is input into a centrifuge to separate and remove the light fat to obtain a defatted liquid;
[0052] S33: Freeze the defatted liquid to 1-4°C to precipitate a small amount of starch, dextrin and fiber fine particles therein, and then use an ultrafiltration membrane to remove impurities to obtain a refined peptide solution.
[0053] In the above implementation steps, most of the wheat bran, starch and a small amount of insoluble protein remaining incompletely reacted in the reaction solution are removed by plate and frame filtration; then a centrifuge is used to remove fat; finally, cryofiltration is used to deeply remove fine particles to obtain a refined peptide solution with improved purity.
[0054] The filter press, centrifuge and other equipment involved in the above-mentioned implementation steps can be selected as needed, and the specific type parameters are not specifically limited, and it is only necessary to meet the above-mentioned impurity removal effect of this application.
[0055] S40: The refined peptide solution is concentrated and dried to obtain a peptide product.
[0056] Specifically include:
[0057] S41: The peptide solution is input into a reduced pressure evaporator to remove most of the water, thereby obtaining a peptide concentrate with a dry matter content of 50%. The peptide solution is evaporated at a low temperature to remove water without causing browning;
[0058] Among them, the inlet air temperature of the spray drying process is 200-230℃, and the outlet air temperature is about 80℃.
[0059] S42: spray drying the peptide concentrate to obtain a peptide product for food; and freeze drying the peptide concentrate to obtain a peptide product for cosmetics and medicines;
[0060] Among them, the drying pressure of freeze-drying treatment is 0.092-0.096Mpa.
[0061] The process route designed in the present application is simple, practical and effective. It completely removes water-soluble impurities before the enzymatic hydrolysis reaction, avoids any steps that may produce water-soluble impurities in the subsequent processing process, and deeply purifies the enzymatic hydrolysis products, thereby producing high-purity peptide finished products.
[0062] Example 2
[0063] Based on the steps of Example 1, the following steps are also included:
[0064] S34: The residue filtered and centrifuged during step S31 to step S33 is dried and crushed and used as a raw material for bio-leather or for the manufacture of compound feed.
[0065] The preparation process of the present application through the above-mentioned implementation steps has no solid waste discharge, realizes the comprehensive resource utilization of brewer's grains, and is environmentally friendly while achieving economic benefits.
[0066] Example 3
[0067] Based on the preparation method of Example 1-2, a polypeptide extract is provided, which contains 85-95% peptide by mass and can be widely used in the fields of food, medicine and skin care, with better economic benefits.
[0068] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the attached claims.
Claims
1. A method for preparing polypeptides using beer wet grains, characterized in that: Includes steps: S10: Clean the beer lees to remove all sugar and other water-soluble substances in the lees; S20: enzymatic treatment of beer wet grains to complete the conversion of proteins into peptides; S30: filtering, defatting and freezing ultrafiltration of the enzymatic hydrolysis product in sequence to obtain a refined peptide solution; S40: The refined peptide solution is concentrated and dried to obtain a peptide product.
2. The method for preparing polypeptides using beer wet grains according to claim 1, characterized in that: In step S30, it includes: S31: inputting the enzymatic hydrolysis product into a plate and frame filter press for filtration to obtain a clear liquid after removing the residue; S32: the clear liquid after the residue is removed is input into a centrifuge to separate and remove the light fat to obtain a defatted liquid; S33: Freeze the defatted liquid to 1-4°C to precipitate a small amount of starch, dextrin and fiber fine particles therein, and then use an ultrafiltration membrane to remove impurities to obtain a refined peptide solution.
3. The method for preparing polypeptides using beer wet grains according to claim 2, characterized in that: The step S30 further includes: S34: The residue filtered and centrifuged during step S31 to step S33 is dried and crushed and used as a raw material for bio-leather or for the manufacture of compound feed.
4. The method for preparing polypeptides using beer wet grains according to claim 1, characterized in that: In step S40, it includes: S41: inputting the peptide solution into a reduced pressure evaporator to remove most of the water, thereby obtaining a peptide concentrate having a dry matter content of 50%; S42: spray-drying the peptide concentrate to obtain a peptide product for food; and / or freeze-drying the peptide concentrate to obtain a peptide product for cosmetics or medicine.
5. The method for preparing polypeptides using beer wet grains according to claim 4, characterized in that: The inlet air temperature of the spray drying process is 200-230°C, and the outlet air temperature is 70-90°C; The drying pressure of the freeze-drying process is 0.092-0.096Mpa.
6. The method for preparing polypeptides using beer wet grains according to claim 1, characterized in that: In step S10: Use 2-4 times the amount of clean water as the beer wet grains to wash it 2-5 times.
7. The method for preparing polypeptides using beer wet grains according to claim 1, characterized in that: Step S20 is specifically as follows: Protease is added to the aqueous solution of beer grains to carry out enzymatic reaction. After the enzymatic reaction is completed, the enzyme is quickly inactivated at a relatively low temperature to avoid dextrinization and saccharification of starch.
8. The method for preparing polypeptides using beer wet grains according to claim 7, characterized in that: The protease is a neutral protease, and the addition ratio of the neutral protease is 0.1-0.5%; And / or, the temperature of the enzymatic reaction is 40-55° C., the pH value is 7.2, and the reaction time is 5-8 hours; And / or, the temperature for inactivating the enzyme is 80° C. and the treatment time is within 10 minutes.
9. A polypeptide extract, characterized in that The polypeptide is prepared by the method for preparing polypeptide using beer wet grains as described in any one of claims 1 to 8.
10. The polypeptide extract according to claim 9, characterized in that The peptide mass in the polypeptide extract is between 85-95%.