Preparation method of compound bacterial fertilizer for promoting growth of hydroponic barley and inhibiting bacteria

Abstract

In the whole process from germination to growth of barley seeds into a plant, some ungerminated seeds show the symptoms of mildewing, root rotting and the like. According to the test, a microbial control method is utilized, firstly, a dominant population is formed around the root system of the plant through mass propagation of microbial beneficial bacteria, and life activities of other harmful bacteria are inhibited; and 2, biological interaction between microbial strains and infectious microbes. The microbial strain has a certain metabolic capability and can generate some active substances capable of inhibiting the growth of infectious microbes, such as antibiotics, protease and the like, and the substances can destroy the key physiological mechanism of the infectious microbes, so that the effect of inhibiting the growth and reproduction of the infectious microbes is achieved. And thirdly, the beneficial bacteria metabolize to generate various substances required by plants, such as small molecular amino acids, growth stimulation substances, vitamins and the like, and the beneficial bacteria continuously propagate and metabolize around the root systems of the plants, continuously and non-excessively provide nutrients for the crops, and promote the growth of the plants. Glucose is added when the bacterial fertilizer is used, the glucose is monosaccharide with the highest energy efficiency, microorganisms can grow and metabolize massively by utilizing energy provided by the glucose, and subsequent field planting, growth and reproduction of hydroponic barley root systems are guaranteed.

Description

Technical Field

[0001] The invention belongs to the field of hydroponic barley ripening, and in particular relates to a method for preparing a composite bacterial fertilizer which can promote the growth of hydroponic barley and inhibit bacteria. Background Art

[0002] Hydroponics is an efficient and environmentally friendly way of growing plants, especially suitable for the production of food crops such as barley. Compared with traditional soil cultivation, hydroponics can better control the nutrients required for plant growth and increase the growth rate and yield of crops. However, the humidity and characteristics of the nutrient solution in the hydroponic environment can easily lead to the growth of pathogenic microorganisms, which in turn cause mold and other diseases in plants, seriously affecting the growth and quality of crops.

[0003] In the production of hydroponic barley, traditional nutrient solution formulas often cannot effectively inhibit the growth of pathogenic microorganisms. Therefore, the development of a bacterial fertilizer that can both promote barley growth and inhibit mold growth has important economic and ecological significance. By applying specific probiotics or fungi to the hydroponic system, it can not only increase the growth rate and disease resistance of barley, but also help improve the microbial balance of the hydroponic environment, thereby reducing the growth of mold. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing a composite bacterial fertilizer that promotes the growth of hydroponic barley and inhibits bacteria, which can effectively promote the growth of hydroponic barley and has the function of inhibiting the growth of pathogens. In order to achieve the above purpose, the technical solution adopted by the present invention is:

[0005] A composite bacterial fertilizer for promoting the growth of hydroponic barley and inhibiting bacteria comprises: Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Azotobacter rotundus, Bacillus brevis, fulvic acid and humic acid.

[0006] 1) Seed soaking: The barley seeds that have been screened by the machine are then screened with a sieve to remove seeds with a width of less than 3mm. At the same time, broken grains, rotten grains, and sand and gravel are removed manually. The screened seeds are fully stirred and washed 2-3 times with tap water, then washed twice with purified water, the washing water is drained, and the seeds are soaked in clean water for 18 hours.

[0007] 2) Sowing / Putting in trays: After soaking the barley seeds, pour out the soaking water, control the moisture, and put 200 g of wet seeds in each tray. Set 4 replicates for each treatment.

[0008] 3) Germination treatment (dark treatment): Place the double-layer trays of each treatment on the shelf of the planting laboratory for dark culture for 2 days (starting from the shelf, a total of 48 hours). The ambient temperature of the planting laboratory is 22±2°C, the humidity is 75±5%, and no supplementary light is provided. During the dark culture stage, each treatment is sprayed with water three times a day at 8:00, 13:00, and 17:00, and each spraying is based on watering thoroughly.

[0009] 4) Light culture: 48 hours after the dark treatment, turn on the planting supplementary light above the shelf, adjust the initial light intensity to 200±20μmol / ㎡ / s, and the light-dark cycle to 12h (the supplementary light period is 6:00-18:00). Light culture for 5-6 days, the ambient temperature of the planting laboratory is 23±1℃, and the humidity is 75±5%. For each treatment, the watering method is the same as the dark treatment. In addition, treatment group 2 is sprayed with compound fertilizer twice in the morning and evening.

[0010] Furthermore, the compound bacterial fertilizer is Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Azotobacter rotundus, Bacillus brevis, fulvic acid, and humic acid.

[0011] Furthermore, the mass ratio of Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Azotobacter rotundifolia, Bacillus pumilus, fulvic acid, and humic acid is (5-10): (5-10): (5-10): (45-55): (25-35): (100-110): (15-25): (60-70): (180-200). Furthermore, when the mixed bacterial fertilizer is used, 20-25 parts of bacterial fertilizer, 25-35 parts of glucose, and 8000-10000 parts of water are used. Furthermore, glucose is added when the bacterial fertilizer is used. Glucose is the most energy-efficient monosaccharide. Microorganisms can use the energy provided by glucose to carry out a large amount of growth metabolism, ensuring the subsequent colonization and growth and reproduction of the root system of hydroponic barley.

[0012] The advantages and beneficial effects of the present invention compared with the prior art are:

[0013] (1) The formula composition of the present invention is reasonable, and the proportion design of each bacterial species in the fermentation fertilizer takes into account the synergistic effect between the bacterial species, thereby improving the stability of the microbial fertilizer.

[0014] (2) The composite bacterial fertilizer of the present invention can promote the stem and leaf height and planting ratio of double-layer tray hydroponic barley, inhibit (slow down) the number of foreign bacteria in the barley seed layer and the contamination area, and increase the dry matter and protein content of barley grass. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings, wherein:

[0016] Figure 1 This is the root system diagram of the back of the water cleaning group according to the embodiment of the present invention

[0017] Figure 2 This is the root system diagram of the back of the bacterial fertilizer group of the embodiment of the present invention

[0018] Figure 3 This is a side root system comparison diagram of an embodiment of the present invention

[0019] Figure 4 A comparison diagram of the seed layer in the embodiment of the present invention DETAILED DESCRIPTION

[0020] The following examples may enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. However, those skilled in the art should understand that various changes may be made to the form and details without departing from the scope of the present invention as defined in the claims.

[0021] In order to facilitate understanding by those skilled in the art, the present invention is further described below in conjunction with specific embodiments.

[0022] Example 1

[0023] 1) Preparation of composite bacterial fertilizer: weigh the following bacterial fertilizers by mass: Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Azotobacter rotundifolia, Bacillus pumilus, fulvic acid, humic acid, according to the mass ratio of (5-10): (5-10): (5-10): (45-55): (25-35): (100-110): (15-25): (60-70): (180-200);

[0024] 2) Preparation of compound fertilizer: weigh fulvic acid and humic acid by mass in a mass ratio of (1-2):(3:4);

[0025] 3) Preparation of compound bacterial fertilizer: the compound bacterial fertilizer and fertilizer are mixed according to the mass ratio, 1-2 parts of compound bacterial fertilizer are mixed with 2-3 parts of compound fertilizer;

[0026] 4) Use of compound bacterial fertilizer: When using this mixed bacterial fertilizer, mix it according to weight: 20-25 parts of bacterial fertilizer, 25-35 parts of glucose, and 8000-10000 parts of water.

[0027] The present invention obtains a composite bacterial fertilizer by mixing bacterial fertilizer and fertilizer in different mass ratios according to the mass ratio, and the composite bacterial fertilizer is mixed and used in a ratio of 20-25 parts of bacterial fertilizer and 25-35 parts of glucose. The present invention has a reasonable formula composition, and the ratio design of each bacterial species in the fermented bacterial fertilizer takes into account the synergistic effect between the bacterial species, thereby improving the stability of the microbial bacterial fertilizer.

[0028] Effect test of the composite bacterial fertilizer of the present invention

[0029] 1. Test materials

[0030] (1) Barley seeds (Australian barley);

[0031] (2) The planting container is a double-layer planting tray, which is used as an open planting container;

[0032] (3) Planting supplementary lighting "grass tower light bar" (red and white light), the initial vertical distance between the light bar and the seeds is 35 cm;

[0033] (4) Barley seed sieve (sieve opening 3 mm);

[0034] (5) The experimental water was purified water made by a laboratory water purifier;

[0035] (6) Compound bacterial fertilizer: self-prepared compound bacterial fertilizer, the concentration is 0.04%.

[0036] 2. Experimental design

[0037] Treatment 1 Seed soaking: After the barley seeds have been screened by a machine, they are screened with a sieve to remove seeds with a width of less than 3 mm. At the same time, broken grains, rotten grains, and sand and gravel are removed manually. After the seeds have been screened, they are fully stirred and washed 2-3 times with tap water, then washed twice with purified water, the washing water is drained, and the seeds are soaked in clean water for 18 hours. Treatment 2 Sowing / Putting in trays: After the barley seeds have been soaked, the soaking water is poured out, the water is drained, and 200g of wet seeds are placed in each tray. Four replicates are set for each treatment.

[0038] Treatment 3 Germination treatment (dark treatment): Place the double-layer trays of each treatment on the shelf of the planting laboratory for dark culture for 2 days (starting from the shelf, a total of 48 hours). The ambient temperature of the planting laboratory was 22±2℃, the humidity was 75±5%, and no supplementary light was used. During the dark culture stage, each treatment was sprayed with water three times a day at 8:00, 13:00, and 17:00, and each spray was based on thorough watering.

[0039] Treatment 4 light culture: 48 hours after the dark treatment, turn on the planting supplementary light above the shelf, adjust the initial light intensity to 200±20μmol / ㎡ / s, and the light-dark cycle to 12h (the supplementary light period is 6:00-18:00). Light culture for 5-6 days, the planting laboratory environment temperature is 23±1℃, and the humidity is 75±5%. For each treatment, the watering method is the same as the dark treatment. In addition, treatment group 2 sprays the compound bacterial fertilizer of Example 1 twice in the morning and evening.

[0040] Treatment 5 Conventional water hydroponic barley

[0041] Treatment 6 blank control group

[0042] According to the above test method, the fertilization results were analyzed from three aspects: shape, yield and quality of hydroponic barley plants:

[0043] For Table 1-3, Figure 1-4 Analysis showed that compared with treatment 5, the barley plants sprayed with the composite bacterial fertilizer of Example 1 had significant growth in shape, yield, and quality, and were not prone to mildew. The seed layer of the water group had more bacteria, while the seed layer of the bacterial fertilizer group had fewer bacteria. The seed layer of the water group had earlier bacteria, which was clearly observed on the fourth day after exposure to light; the seed layer of the bacterial fertilizer group had bacteria, which was clearly observed on the fifth day. This shows that the composite bacterial fertilizer of the present invention can promote the growth of hydroponic barley and is not prone to mildew.

[0044] This is because beneficial bacteria metabolize and produce a variety of substances needed by plants, such as small molecule amino acids, growth stimulating substances, vitamins, etc., and beneficial bacteria continue to multiply and metabolize around the plant roots, continuously and non-excessively providing nutrients to crops and promoting plant growth. Fulvic acid and humus contain trace elements that are beneficial to plants, which can promote plant growth, increase yield and quality, and reduce water pollution during hydroponics and improve water quality. Glucose is added when using bacterial fertilizer. Glucose is the most energy-efficient monosaccharide. Microorganisms can use the energy provided by glucose to carry out a large amount of growth metabolism, ensuring the subsequent colonization and growth and reproduction of the hydroponic barley roots.

[0045] Table 1 Height of hydroponic barley in plot

[0046] Grouping Average plant height / cm Average root thickness / cm Shimizu 15.38 2.83 Bacteria fertilizer 16.13 2.87

[0047] Table 2 Weight of hydroponic barley in plot

[0048] Grouping Dry seed weight / g Average fresh weight / g Planting ratio Shimizu 133.3 920.5 6.91 Bacteria fertilizer 133.3 975.5 7.32

[0049] Table 3 Determination of components of hydroponic barley in plots

[0050] Grouping Dry matter content Protein content (dry basis) Shimizu 9.91％ 17.04％ Bacteria fertilizer 10.27％ 17.62％

[0051] The data in Tables 1-3 are the average values ​​of three tests.

[0052] It should be understood that the purpose of these embodiments is only to illustrate the present invention and is not intended to limit the protection scope of the present invention. In addition, it should also be understood that after reading the technical content of the present invention, those skilled in the art can make various changes, modifications and / or variations to the present invention, and all of these equivalent forms also fall within the protection scope defined by the claims attached to this application.

Claims

1. A method for preparing a composite bacterial fertilizer that promotes the growth of hydroponic barley and inhibits bacteria, characterized in that: The following steps are involved: 1) The barley seeds that have been screened by the machine are then screened with a sieve to remove seeds with a width of less than 3mm; at the same time, broken grains, rotten grains, and sand and gravel are removed manually; the screened seeds are fully stirred and washed 2-3 times with tap water, then washed twice with purified water, the washing water is drained, and the seeds are soaked in clean water for 18 hours; 2) After soaking the barley seeds, pour out the soaking water, control the moisture, and put 200g of wet seeds in each tray, with 4 replicates for each treatment; 3) Place the double-layer trays of each treatment on the shelf of the planting laboratory for dark cultivation for 2 days (starting from the time of placing on the shelf, a total of 48 hours); the ambient temperature of the planting laboratory is 22±2℃, the humidity is 75±5%, and no supplementary light is provided; during the dark cultivation stage, each treatment is sprayed with water three times a day at 8:00, 13:00, and 17:00, and each spraying is based on watering thoroughly; 4) 48 hours after the dark treatment, turn on the planting supplementary light above the shelf, adjust the initial light intensity to 200±20μmol / ㎡ / s, and the light-dark cycle to 12h (the supplementary light period is 6:00-18:00); culture under light for 5-6 days, the ambient temperature of the planting laboratory is 23±1℃, and the humidity is 75±5%; the watering method for each treatment is the same as the dark treatment; in addition, treatment group 2 is sprayed with self-prepared bacterial fertilizer twice in the morning and evening.

2. The method according to claim 1, characterized in that: Self-mixed bacterial fertilizers include: Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Bacillus brevis, fulvic acid and humic acid, and glucose.

3. The method according to claim 2, characterized in that: The mass ratios of the Bacillus amyloliquefaciens, Bacillus subtilis, Trichoderma harzianum, Bacillus licheniformis, Trichoderma viride, Azotobacter rotundifolia, Bacillus pumilus, fulvic acid and humic acid, and glucose are (5-10): (5-10): (5-10): (45-55): (25-35): (100-110): (15-25): (60-70): (180-200).

4. The method according to claim 3, characterized in that: Weigh fulvic acid and humic acid by mass in the ratio of (1-2):(3:4).

5. The method according to claim 4, characterized in that: The compound bacterial fertilizer and the fertilizer are mixed according to a mass ratio, 1-2 parts of the compound bacterial fertilizer are mixed with 2-3 parts of the compound fertilizer.

6. The method according to claim 5, characterized in that: When the bacterial fertilizer is mixed for use, the mixture is mixed according to weight: 20-25 parts of bacterial fertilizer, 25-35 parts of glucose, and 8000-10000 parts of water.

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