Multi-element efficient cultivation method for suberect spatholobus stem under forest

Abstract

The invention belongs to the technical field of suberect spatholobus stem cultivation, and particularly relates to an under-forest suberect spatholobus stem multi-element efficient cultivation method which comprises the following steps: S1, selecting a low-hill pest-free pine forest; s2, caulis spatholobi is cultivated close to the root of the pine forest; s3, planting radix fici simplicissimae and radix asparagi in an open field of the pine forest; wherein in the cultivation process, the suberect spatholobus stem is irrigated with a high-activity bactericidal agent; the high-activity bactericidal agent is prepared by compounding phenazino-1-carboxylic acid, fenaminstrobin and physcion. The suberect spatholobus stem, the radix fici simplicissimae and the radix asparagi are cultivated under the pine forest to form a multi-element efficient under-forest suberect spatholobus stem cultivation mode, and the space can be effectively and fully utilized. In the cultivation process, a high-activity bactericide formed by compounding phenazino-1-carboxylic acid, fenaminstrobin and physcion is used, the suberect spatholobus stem root rot can be efficiently prevented and treated, the use amount of the bactericide can be reduced, the problem of pesticide residues can be effectively solved, meanwhile, the risk of drug resistance can be reduced, and then support is provided for multi-element efficient cultivation of suberect spatholobus stems under the forest.

Description

Technical Field

[0001] The invention belongs to the technical field of Millettia spatholobi cultivation, and particularly relates to a multi-element and efficient cultivation method of Millettia spatholobi under forests. Background Art

[0002] Millettia spatholobi is a traditional Chinese medicinal material with the effects of promoting blood circulation, regulating menstruation and relieving pain, and relaxing muscles and tendons. The wild resources of Millettia spatholobi are mainly distributed in a few areas such as Guangxi, Guangdong and Yunnan. However, due to the large market demand and excessive collection, the wild resources of Millettia spatholobi are becoming increasingly scarce. The use of the multi-element and efficient cultivation method of Millettia spatholobi under the forest can effectively alleviate the above problems.

[0003] The prior art discloses relevant contents of multi-element and efficient cultivation of Millettia spatholobi under forests, such as patent CN113141953B, which discloses a four-element ecological and efficient cultivation model method under forests, wherein Millettia spatholobi is cultivated near the roots of pine forests in low-hill ecological public welfare pine forests using segmented precise cultivation and control technology, and Alpinia oxyphylla and Asparagus cochinchinensis are cross-planted on multiple layered and flattened slopes in open areas under pine forests. This patent adopts a four-element cultivation model under forests, fully utilizing space, planting economic crops under forests under low-hill ecological public welfare pine forests, and obtaining a variety of medicinal materials such as Millettia spatholobi, Alpinia oxyphylla and Asparagus cochinchinensis, effectively improving unit yield and quality, with significant economic benefits.

[0004] However, during the planting process of Millettia reticulata, Millettia reticulata is susceptible to diseases and pests, especially root rot. Root rot is a common disease of Millettia reticulata. After the onset of the disease, the roots turn dark brown, the root bark gradually rots, and the branches and leaves wilt until they die. During the planting process, thiophanate-methyl can be used for root irrigation or benomyl spraying. However, in the process of preventing agricultural diseases, drug resistance and pesticide residues are issues that cannot be ignored. Pathogens have developed a high resistance to a variety of existing agents, and the use of large doses will cause excessive pesticide residues. Therefore, it is necessary to demand new and efficient agents for the prevention and treatment of Millettia reticulata root rot. Summary of the invention

[0005] The purpose of the present invention is to provide a multi-element and efficient cultivation method for Millettia spatholobi under forests, which uses a highly active fungicide to irrigate the roots of Millettia spatholobi during the cultivation process, can effectively prevent and control Millettia spatholobi root rot, and provide an effective guarantee for the healthy growth of Millettia spatholobi.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A multi-element and high-efficiency cultivation method of Millettia spatholobi under forests comprises the following steps:

[0008] S1. Choose a pest-free pine forest on a low hill;

[0009] S2. Plant Millettia spatholobi near the roots of pine forests;

[0010] S3. Leveling multiple soil slopes in layers in an open area of ​​a pine forest, and cross-planting five-fingered peach and asparagus on each soil slope;

[0011] In the process of cultivation, a high-activity fungicide is used to irrigate the roots of Millettia reticulata; the high-activity fungicide is a compound of sinocycline, enoxamyl and physcion; the sinocycline is a 1% sinocycline suspension; the enoxamyl is a 5% enoxamyl emulsifiable concentrate; the physcion is a 0.8% physcion suspension.

[0012] More specifically, in step S1, the age of the pine trees in the forest is over 15 years.

[0013] More specifically, the specific operation in step S2 is: when the length of the part of the Millettia vine that crawls toward the ground reaches 0.7-1.3m, the soil is compacted at 1m to allow the Millettia vine compacted by the soil to take root and grow; when the length of the Millettia vine that grows later reaches 0.7-1.3m, the soil is compacted at 1m to allow the Millettia vine compacted by the soil to take root and grow, and the cycle is repeated.

[0014] More specifically, in step S3, the length of each soil slope is 15 m and the width is 5 m.

[0015] More specifically, the mass ratio of the shinazomib, enoxamyl and physaccharin methyl ether is 1:1:1-9.

[0016] More specifically, the mass ratio of the shinazomib, enoxamyl and physaccharin methyl ether is 1:1:3.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention cultivates Millettia spatholobi, Prunus mume and Asparagus cochinchinensis under a pine forest to form a multi-element and efficient Millettia spatholobi under the forest, which can effectively make full use of space. In the cultivation process, a highly active fungicide compounded by sinocyclamycin, enoxamyl and physcion is used to effectively prevent and treat Millettia spatholobi root rot, thereby reducing the amount of agent used, thereby effectively solving the problem of pesticide residues, and at the same time reducing the risk of drug resistance, thereby providing support for multi-element and efficient cultivation of Millettia spatholobi under the forest. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is described clearly and completely below. Based on the embodiments of the present invention, all other embodiments obtained by the technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1

[0021] 1.1 Test reagents

[0022] 1% shinazolin suspension (Hubei Tianzenong Bioengineering Co., Ltd.), 5% enoxamyl emulsifiable concentrate (Shenyang Kechuang Chemicals Co., Ltd.), 0.8% physcion suspension (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.)

[0023] 1.2 Test strains

[0024] Samples of Millettia spatholobi root rot were collected, and the pathogen was isolated, purified and identified in the laboratory, and it was determined that the pathogen was Fusarium oxysporum.

[0025] The test strain was inoculated onto PDA medium and cultured in a 25°C incubator for 3 days. A 5 mm bacterial cake was then punched along the edge of the colony with a borer for later use.

[0026] 1.3 Compound test

[0027] The test agent was diluted with 0.05% Tween-80 aqueous solution to a single-dose stock solution with a mass concentration of 5000 mg / L; multiple groups of proportions were set up, and the single-dose stock solution and the proportioned mixture were diluted with 0.05% Tween-80 aqueous solution to 6 mass concentration gradients. The above agents were prepared and used immediately.

[0028] Mix the drug solution and the PDA medium melted and cooled to 55°C in a sterile conical flask at a volume ratio of 1:10, and then pour into a 9cm diameter culture dish, 12mL per culture dish. After the drug-containing culture medium is cooled, a drug-containing plate is formed. Inoculate a beaten bacterial cake in the center of the drug-containing plate, cover the dish and place it in a 25°C incubator for culture. Repeat each mass concentration of drug solution 3 times, and each repeat 3 drug-containing plates. Set the treatment of 0.05% Tween-80 aqueous solution as a blank control.

[0029] When the colony diameter of the blank control grows to about 6 cm, the colony diameter is measured with a caliper. The diameter of each colony is measured vertically once using the cross method, and the average value is taken to calculate the mycelium growth inhibition rate.

[0030]

[0031] 1.4 Results Analysis

[0032] Regression analysis was performed on the logarithmic value of the drug solution concentration and the probability value of the mycelium growth inhibition rate to obtain the regression equation and the inhibitory concentration EC50 value. The co-toxicity coefficient CTC value was calculated using the Sun Yunpei method, and the synergistic effect type of drug mixing was evaluated based on the co-toxicity coefficient CTCT value: CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect. The results are shown in Table 1-2.

[0033] Table 2 The combined effect of shinazolin, enoxamyl and physoxaline on Millettia reticulata root rot

[0034]

[0035] As shown in Table 1, the inhibitory concentrations of shinazomib, enoxamyl and physophanol on the mycelial growth of Fusarium oxysporum, the root rot pathogen of Millettia spatholobi, were 16.0836 mg / L, 3.6097 mg / L and 9.5728 mg / L, respectively. When the three were mixed in pairs at a mass ratio of 1:1, the co-toxicity coefficient of the combination of shinazomib and physophanol on the inhibition of the mycelial growth of Fusarium oxysporum, the root rot pathogen of Millettia spatholobi, was 125.5408, showing a synergistic effect, and the remaining two groups of compound combinations showed an additive effect.

[0036] When the three are compounded in a mass ratio of 1:1:1-12, the compound combination of 1:1:12 shows an additive effect, and the co-toxicity coefficients of the other compound combinations for inhibiting the growth of mycelium of Fusarium oxysporum, the root rot pathogen of Millettia spatholobi, range from 1327709 to 465.1364, which are all greater than 125.5408 of the compound combination of shinazomib and physaccharin methyl ether. In particular, when shinazomib, enoxamyl and physaccharin methyl ether are compounded in a mass ratio of 1:1:3, the co-toxicity coefficient value is the largest and the synergistic effect is most obvious. In the present invention, shinazomib, enoxamyl and physaccharin methyl ether are compounded to effectively prevent and treat Millettia spatholobi root rot, based on which the amount of agent used can be reduced, thereby effectively solving the problem of pesticide residues, and at the same time, the risk of drug resistance can be reduced.

[0037] Example 2

[0038] A multi-element and high-efficiency cultivation method of Millettia spatholobi under forests comprises the following steps:

[0039] S1. Select a low-lying, pest-free pine forest with trees over 15 years old;

[0040] S2. Cultivate Millettia spatholobi near the root of the pine forest, specifically: Millettia spatholobi grows along the trunks of the pine forest, and when the length of the part of Millettia spatholobi that crawls toward the ground reaches 0.7-1.3m, compact the soil at 1m to allow the Millettia spatholobi compacted by the soil to take root and grow; when the length of the later-grown Millettia spatholobi reaches 0.7-1.3m, compact the soil at 1m to allow the Millettia spatholobi compacted by the soil to take root and grow, and so on;

[0041] S3. In an open area in a pine forest, multiple slopes were leveled and stratified, and five-fingered peach and asparagus were planted alternately on each slope; each slope was 15m long and 5m wide;

[0042] In the process of cultivation, the highly active fungicide is diluted 500-800 times with clean water and then irrigated into the roots of Millettia reticulata; the highly active fungicide is compounded by shinazomib, enoxamyl and physaccharin methyl ether; the shinazomib is a 1% shinazomib suspension; the enoxamyl is a 5% enoxamyl emulsifiable concentrate; the physaccharin methyl ether is a 0.8% physaccharin methyl ether suspension; the mass ratio of the shinazomib, enoxamyl and physaccharin methyl ether is 1:1:3.

Claims

1. A multi-element and efficient cultivation method of Millettia spatholobi under forest, characterized in that: The following steps are involved: S1. Choose a pest-free pine forest on a low hill; S2. Plant Millettia spatholobi near the roots of pine forests; S3. Leveling multiple soil slopes in layers in an open area of ​​a pine forest, and cross-planting five-fingered peach and asparagus on each soil slope; In the process of cultivation, a high-activity fungicide is used to irrigate the roots of Millettia reticulata; the high-activity fungicide is a compound of sinocycline, enoxamyl and physcion; the sinocycline is a 1% sinocycline suspension; the enoxamyl is a 5% enoxamyl emulsifiable concentrate; the physcion is a 0.8% physcion suspension.

2. The multi-element and high-efficiency cultivation method of Millettia spatholobi according to claim 1, characterized in that: In step S1, the age of the pine trees in the forest is over 15 years.

3. The multi-element and high-efficiency cultivation method of Millettia spatholobi according to claim 1, characterized in that: The specific operation in step S2 is: when the length of the part of the Millettia vine that crawls toward the ground reaches 0.7-1.3m, the soil is compacted at 1m to allow the Millettia vine compacted by the soil to take root and grow; when the length of the Millettia vine that grows later reaches 0.7-1.3m, the soil is compacted at 1m to allow the Millettia vine compacted by the soil to take root and grow, and the cycle is repeated.

4. The multi-element and high-efficiency cultivation method of Millettia spatholobi according to claim 1, characterized in that: In step S3, the length of each soil slope is 15m and the width is 5m.

5. The multi-element and high-efficiency cultivation method of Millettia spatholobi according to claim 1, characterized in that: The mass ratio of the shinazomib, enoxamyl and physaccharin methyl ether is 1:1:1-9.

6. The multi-element and high-efficiency cultivation method of Millettia spatholobi according to claim 1, characterized in that: The mass ratio of the shinazomib, enoxamyl and physaccharin methyl ether is 1:1:3.

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