Testing device for identifying behaviors of insects on magnetic induction mechanism based on free radicals

Abstract

The invention discloses a behavior testing device for identifying a magnetic induction mechanism of insects based on free radicals. The behavior testing device is characterized by comprising a flight orientation testing system, a signal acquisition and processing system and a radio frequency magnetic field signal regulation and control system, wherein the radio frequency magnetic field signal regulation and control system is used for modulating and generating a set radio frequency magnetic field signal and acting on an insect to be tested in the flight orientation test system; the flight orientation test system is used for placing or suspending to-be-tested insects, and the to-be-tested insects sensing the set radio frequency magnetic field signals can make flight orientation behavior responses; the signal collecting and processing system is used for collecting behavioral data, generated by the flight orientation testing system, of the to-be-tested insects and analyzing and processing the behavioral data. The behavior testing device provided by the invention can control the electromagnetic coil to generate a specific radio frequency magnetic field signal through the signal generator to perform an experiment, and acquires flight behavior data of the insect to be tested in the experiment to identify the magnetic induction and magnetic orientation mechanism of the insect based on the free radical pair.

Description

Technical Field

[0001] The present invention belongs to the technical field of insect magnetic induction mechanism research, and in particular to the technical field of exploring the magnetic induction mechanism and magnetic orientation mechanism of migratory insects based on free radical pairs. Specifically, it is a behavioral testing device for identifying insect magnetic induction mechanism based on free radical pairs. Background Art

[0002] Insect orientation refers to the behavior and ability of insects to actively adjust their direction of travel with the help of directional beacons such as the geomagnetic field, polarized light, and the sun's position during walking or flying. Many insects, such as fall armyworm, grass moth, oriental armyworm and other major agricultural pests, have migratory habits. Large insects often have autonomous directional behavior during migration. The same directional pattern causes a large number of pests to land in a relatively small area, causing large-scale damage to field crops. Therefore, studying the orientation mechanism of insects is helpful to determine their migration trajectory and landing area, and provide a basis for the prediction and forecast of migratory pests. At present, it is believed that there are several types of insect orientation mechanisms, including solar compass orientation, geomagnetic orientation, star sign orientation, polarized light orientation, etc. (Wu Xianfu, Feng Hongqiang, Xue Fangsen, et al. Orientation behavior of insects during migration[J]. Plant protection , 2006(05): 1-5). Among them, since the Earth's magnetic field is stable and ubiquitous, and is also an important part of bionics (magnetic induction device) research, the magnetic induction mechanism and magnetic orientation mechanism of animals are currently being widely studied.

[0003] In the ocean, salmon and turtles can return to the streams or beaches where they were born thousands of kilometers away; Arctic terns can breed near the Arctic and spend the winter near the Antarctic; seabirds fly more than 100,000 kilometers each year, but return to fixed islands to breed; Australia's bugoon flies south to the southern caves in the spring to escape the heat, and flies north to return to their birthplace in the fall. How do these animals sense geomagnetic clues to help them navigate? At present, there are three mainstream hypotheses for animal magnetoreception: (1) magnetoreception mechanism based on free radical pairs (2) magnetoreception mechanism based on magnetic particles; (3) electromagnetic induction mechanism; (Mouritsen H. Long-distance navigation and magnetoreception inmigratory animals [J]. Nature, 2018, 558(7708): 50-59). At present, there are two main devices for insect simulation orientation, one based on camera and the other based on encoder technology, but both are only used for single flight orientation behavior detection, and there are few reports on magnetic induction mechanism system detection devices. Given that radio frequency magnetic fields can physically interfere with the magnetic induction process based on the free radical pair mechanism (Leberecht, B. et al. Upper bound for broadbandradiofrequency field disruption of magnetic compass orientation in night-migratory songbirds. Proc Natl Acad Sci USA , 120, e2301153120), based on this principle, this patent aims to propose a set of behavioral testing devices for verifying the insects' magnetic induction mechanism based on free radicals. Summary of the invention

[0004] The purpose of the present invention is to provide a behavior testing device for identifying insects based on the magnetic induction mechanism of free radicals in order to solve the problems existing in the prior art.

[0005] The purpose of the present invention is to be solved by the following technical solutions: A behavior testing device for identifying insects based on the free radical magnetic induction mechanism, characterized in that: the behavior testing device includes a flight orientation testing system, a signal acquisition and processing system, and a radio frequency magnetic field signal control system; wherein, The radio frequency magnetic field signal control system is used to modulate and generate a set radio frequency magnetic field signal and act on the insects to be tested in the flight orientation test system; The flight orientation test system is used to place the insects to be tested. The insects to be tested that sense the radio frequency magnetic field signal can make a flight behavior response in the flight orientation test system and generate flight behavior data based on the flight behavior response. The signal acquisition and processing system is used to collect the flight behavior data of the insects to be tested generated by the flight orientation test system and analyze and process it.

[0006] The flight orientation test system comprises a test box, an electromagnetic shielding cover, a micro-bearing, a carbon fiber rod and an insect ring. The electromagnetic shielding cover is arranged on the cover of the test box and a micro-bearing is embedded in the bottom of the cavity of the electromagnetic shielding cover. An optical encoder and an interlocking device are arranged in the inner cavity of the electromagnetic shielding cover. The top end of the carbon fiber rod embedded in the micro-bearing is upwardly connected to the interlocking device, the carbon fiber rod extends downward into the body of the test box and an insect ring is arranged at the bottom of the carbon fiber rod. The insect ring is used to fix the back of the insect to be tested.

[0007] The interlocking device is used to connect the bearing and the carbon fiber rod.

[0008] The miniature bearing is of model XUDZ and is produced in Japan. The number of rolling elements is single row, the rolling bearing seat is made of bearing steel, and the specifications are 1mm in inner diameter, 3mm in outer diameter, and 1mm in height.

[0009] The optical encoder is fixedly installed in the inner cavity of the electromagnetic shielding cover, and the interlocking device arranged in the inner cavity of the electromagnetic shielding cover can rotate with the carbon fiber rod. The optical encoder is located above the inner cavity of the shielding cover, and the interlocking device is located below the inner cavity of the shielding cover.

[0010] A code disc is placed on the interlocking device. When the carbon fiber rod rotates, the code disc is driven to rotate. The optical encoder receives the rotation information of the code disc and transmits it to the single-chip microcontroller.

[0011] The insect hanging ring comprises a polyfluoro tube and a U-shaped copper wire, the U-shaped copper wire is arranged at the bottom of the polyfluoro tube and the top of the polyfluoro tube is connected to a carbon fiber rod, and the size of the U-shaped copper wire is determined according to the body size of the insect to be tested.

[0012] The test box is made of nylon material by 3D printing.

[0013] The signal acquisition and processing system includes a single-chip microcontroller and a computer. The single-chip microcontroller receives the flight behavior data of the insect to be tested transmitted by the optical encoder in the flight orientation test system through a shielded signal line. The single-chip microcontroller is connected to the computer through a data line of a USB interface, and the computer is used to analyze the flight behavior data of the insect to be tested.

[0014] The single-chip microcontroller is used for transmitting and processing data.

[0015] The computer is a portable workstation.

[0016] The radio frequency magnetic field signal control system includes an electromagnetic coil and a signal generator. The electromagnetic coil is wrapped on the box body of the flight orientation test system and the insect to be tested in the box body is located at the center of the electromagnetic coil; the electromagnetic coil is connected to the BNC adapter through a shielded signal line, and the BNC adapter is installed at the interface of the signal generator.

[0017] The signal generator is used to generate an electromagnetic signal within a radio frequency range.

[0018] The electromagnetic coil comprises a coil made of nylon material, on which an enameled copper wire is wound to form the electromagnetic coil, and the head and tail of the enameled copper wire are respectively connected to the shielded signal line.

[0019] The radio frequency magnetic field signal control system further comprises a Faraday cage, and the electromagnetic coils of the flight orientation test system and the radio frequency magnetic field signal control system are both arranged in the Faraday cage.

[0020] The top of the Faraday cage is covered with a copper wire mesh and the Faraday cage is grounded to avoid interference and allow light to pass through.

[0021] When the insect to be tested is flying, the insect hanging ring drives the carbon fiber rod, the micro bearing and the interlocking device to rotate, and the code disk also rotates with the interlocking device. The flight behavior data of the insect to be tested is transmitted to the optical encoder according to the number of rotations and information of the code disk. The optical encoder transmits the flight behavior data of the insect to be tested to the single-chip microcontroller, and the single-chip microcontroller outputs it to the computer.

[0022] Compared with the prior art, the present invention has the following advantages: The behavioral testing device provided by the present invention can control the electromagnetic coil to generate a specific radio frequency magnetic field signal for experiments through a signal generator, and collect the flight behavior data of the insects to be tested in the experiment to verify the magnetic orientation mechanism of the insect free radical pairs. The structure is simple, the operation is simple, and the experimental repeatability is high. The signal generator is directly connected to the coil and has an automated and intelligent control system. It can directly adjust the required conditions according to the required experimental environment, reduce the error rate and improve work efficiency. At the same time, the signal generator has multiple functions and can adapt to different experimental conditions, and is suitable for popularization and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Attached Figure 1 A schematic diagram of the structure of a behavior testing device for identifying insects based on a free radical magnetic induction mechanism provided by the present invention; Attached Figure 2 A schematic diagram of the structure of the connection part between the flight orientation test system and the signal acquisition and processing system provided by the present invention; Attached Figure 3 A schematic diagram of the structure of the radio frequency magnetic field signal control system provided by the present invention; Attached Figure 4 A schematic diagram of the structure of the insect hanging ring provided by the present invention; Attached Figure 5 The group orientation of lepidopteran insects in the verification example provided by the present invention under natural conditions and after applying a radio frequency magnetic field; Attached Figure 6 This is a diagram of the flight stability of lepidopteran insects under natural conditions and after applying a radio frequency magnetic field, which is a verification example provided by the present invention.

[0024] Among them, 1 is the box cover; 2 is the optical encoder; 3 is the interlocking device; 4 is the electromagnetic shielding cover; 5 is the miniature bearing; 6 is the carbon fiber rod; 7 is the computer; 8 is the single-chip microcontroller; 9 is the box; 10 is the electromagnetic coil; 11 is the signal generator; 12 is the Faraday cage; 13 is the BNC adapter; 14 is the insect to be tested; 15 is the polytetrafluoroethylene tube; 16 is the U-shaped copper wire; 17 is the shielded signal line. DETAILED DESCRIPTION

[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] like Figure 1-4 As shown: a behavior testing device for identifying insects based on the free radical magnetic induction mechanism, the behavior testing device includes a flight orientation testing system, a signal acquisition and processing system and a radio frequency magnetic field signal control system; wherein the radio frequency magnetic field signal control system is used to modulate and generate a set radio frequency magnetic field signal and act on the insect to be tested 14 in the flight orientation testing system; the flight orientation testing system is used to place the insect to be tested 14, and the insect to be tested 14 that senses the radio frequency magnetic field signal can make a flight behavior response in the flight orientation testing system and generate flight behavior data based on the flight behavior response; the signal acquisition and processing system is used to collect the flight behavior data of the insect to be tested 14 generated by the flight orientation testing system and analyze and process it.

[0027] like Figure 1 , Figure 2 , Figure 4 As shown, the flight orientation test system includes a test box, an electromagnetic shielding cover 4, a micro bearing 5, a carbon fiber rod 6 and an insect ring. The test box includes a box cover 1 and a box body 9. The electromagnetic shielding cover 4 is arranged on the box cover 1 of the test box and a micro bearing 5 is embedded at the bottom of the cavity of the electromagnetic shielding cover 4. An optical encoder 2 and an interlocking device 3 are arranged in the inner cavity of the electromagnetic shielding cover 4. The optical encoder 2 is fixedly installed in the inner cavity of the electromagnetic shielding cover 4 and the interlocking device 3 arranged in the inner cavity of the electromagnetic shielding cover 4 can rotate with the carbon fiber rod 6; the top of the carbon fiber rod 6 embedded in the micro bearing 5 is upwardly connected to the interlocking device 3, the carbon fiber rod 6 extends downward into the box body 9 of the test box, and an insect ring is arranged at the bottom of the carbon fiber rod 6, and the insect ring is used to fix the back of the insect 14 to be tested; the insect ring includes a polyfluoro tube 15 and a U-shaped copper wire 16, the U-shaped copper wire 16 is arranged at the bottom of the polyfluoro tube 15 and the top of the polyfluoro tube 15 is connected to the carbon fiber rod 6, and the size of the U-shaped copper wire 16 is determined according to the body size of the insect 14 to be tested.

[0028] like Figure 1 , Figure 2 As shown, the signal acquisition and processing system includes a single-chip microcontroller 8 and a computer 7. The single-chip microcontroller 8 receives the flight behavior data of the insect to be tested 14 transmitted by the optical encoder 2 in the flight orientation test system through a shielded signal line 17. The single-chip microcontroller 8 is connected to the computer 7 through a data line, and the computer 7 is used to analyze the flight behavior data of the insect to be tested 14.

[0029] like Figure 1 , Figure 3As shown, the radio frequency magnetic field signal control system includes an electromagnetic coil 10 and a signal generator 11. The electromagnetic coil 10 is wrapped on the box 9 of the flight orientation test system, and the insect to be tested 14 in the box 9 is located at the center of the electromagnetic coil 10; the electromagnetic coil 10 is connected to the BNC adapter 13 through the shielded signal line 17, and the BNC adapter 13 is installed at the interface of the signal generator 11. In other words, the electromagnetic coil 10 includes a nylon coil, and an enameled copper wire is wound on the coil to form the electromagnetic coil 10, and the head and tail of the enameled copper wire are respectively connected to the shielded signal line 17.

[0030] like Figure 1 , Figure 3 As shown, the radio frequency magnetic field signal control system also includes a Faraday cage 12, and the electromagnetic coils 10 of the flight orientation test system and the radio frequency magnetic field signal control system are both arranged in the Faraday cage 12; a further preferred option is that a copper wire mesh is covered on the top of the Faraday cage 12 and the Faraday cage is grounded. Example

[0031] The following is a specific example to further illustrate a behavior testing device for identifying insects based on the magnetic induction mechanism of free radicals provided by the present application.

[0032] like Figure 1-4As shown, a behavior test device for identifying insects based on the free radical magnetic induction mechanism comprises a cylindrical box body 9 (30 cm high, 22 cm inner diameter, 23 cm outer diameter), a circular box cover 1 (24 cm inner diameter, 25 cm outer diameter) on the top of the box body 9, the box cover 1 and the box body 9 are both made of nylon with low light transmittance; an electromagnetic shielding cover 4 is placed at the center of the box cover 1, a protruding portion of the electromagnetic shielding cover 4 is embedded in the box cover 1 and the protruding portion of the cover passes through the box cover 1 downward, an optical encoder 2 is pasted on the upper part of the interior of the electromagnetic shielding cover 4, and a An interlocking device 3 is provided below the optical encoder 2, a carbon fiber rod 6 is connected below the interlocking device 3, a micro bearing 5 is embedded in the protruding portion of the electromagnetic shielding cover 4, the micro bearing 5 is connected to the interlocking device 3 through the carbon fiber rod 6, the lower end of the carbon fiber rod 6 is connected to the insect 14 to be tested through an insect hanging ring composed of a polytetrafluoroethylene tube 15 and a U-shaped copper wire 16, the polytetrafluoroethylene tube 15 is a hanging ring arm, the front end of the insect hanging ring is bent from a thin copper wire into a U-shaped copper wire 16, the U-shaped copper wire 16 can be fixed to the back of the insect, the distance from the bottom of the micro bearing 5 to the lowest end of the carbon fiber rod is 12.5 cm, and the length of the insect hanging ring is 1 cm; The optical encoder 2 is connected to the single-chip microcontroller 8 through a shielded signal line 17. The single-chip microcontroller 8 is used to collect flight behavior data from the optical encoder 2 and transmit it to the computer 7. The cylindrical box 9 in the experimental area is covered with a Faraday cage 12 on the outside to shield the interference of external radio frequency magnetic field signals on the experiment. An electromagnetic coil 10 is attached to the outside of the box 9. The electromagnetic coil 10 is composed of enameled copper wire wrapped around a nylon annular coil. The enameled copper wire with a wire diameter of 2mm is wound in two layers, with 15 turns in each layer. After the enameled copper wire is wound around the annular coil, the outside of the annular coil is wrapped with electrical tape, leaving only the enameled copper wire. The head and tail of the copper wire are wrapped, and the height of the wound electromagnetic coil 10 is 4 cm. The upper end of the electromagnetic coil 10 is located at 16.5 cm of the barrel height, the lower end is located at 12.5 cm of the barrel height, and the center is at 14.5 cm of the barrel height, ensuring that the insect 14 to be tested can be located at the center of the electromagnetic coil 10 to avoid errors; use sandpaper to grind off the copper wire paint at the head and tail of the enameled copper wire, and the polished enameled copper wire head and tail are respectively connected to one end of the shielded signal line 17, and the other end of the shielded signal line 17 is respectively connected to the BNC adapter 13, and finally connected to the interface of the signal generator 11. The magnetic field generated by the electromagnetic coil 10 is adjusted by the signal generator 11 for verification test. The entire system is carried out in a Faraday cage 12 to shield the interference of external magnetic field noise. Verification Example

[0033] When the behavior test device for identifying insects based on the magnetic induction mechanism of free radicals provided by the present application is used, during the experiment, the control computer and the signal source generator are kept at a certain distance from the experimental equipment to prevent the magnetic field generated by the electronic equipment from having a certain influence on the insects to be tested. The insect to be tested 14 is an adult of a lepidopteran insect, and the adult body length is measured to be 15-20mm, and the width of the thorax and abdomen junction is 2-4mm. The U-shaped copper wire 16 of the insect hanging ring is bent into a U-shape of about 3mm using a thin copper wire, and the fluff on the thorax and abdomen of the adult is removed with a brush. The adult and the U-shaped copper wire 16 are connected together with 502 glue, and the U-shaped copper wire 16 is fixed on the polytetrafluoroethylene tube 15 to form an insect hanging ring. The box cover 1 is opened to connect the top of the polytetrafluoroethylene tube 15 with the carbon fiber rod 6 under the interlocking device 3, and then the box cover 1 is flatly covered. At this time, the lepidopteran insect as the insect to be tested 14 can fly under the insect hanging ring; the optical encoder 2 and the single-chip microcontroller 8 are connected, and the code disk on the interlocking device 3 records the lepidopteran insect according to the continuous rotation of the carbon fiber rod 6. The instantaneous direction is reflected to the optical encoder 2; the universal source signal generator 11 is turned on, and the two ends of the electromagnetic coil 10 are connected to the universal source signal generator 11 through the BNC adapter 13. The universal source signal generator 11 is adjusted to achieve the set experimental conditions. At this time, the insect body of the lepidopteran insect is placed in the center of the magnetic field generated by the electromagnetic coil 10 to sense the signal and make changes. The lepidopteran insect rotates the ring and drives the carbon fiber rod 6 by flying, and transmits it to the micro bearing 5 and the interlocking device 3. The optical encoder 2 determines the flight behavior data of the lepidopteran insect according to the number of turns and information of the code disk on the interlocking device 3, and then transfers it to the computer 7 through the single-chip microcontroller 8. These lepidopteran insects fly stably and can freely rotate to any azimuth during the test. The data is collected every 200ms and analyzed after the collection is completed. If the lepidopteran insect is oriented when not exposed to the radio frequency magnetic field, and no longer oriented after the radio frequency magnetic field acts, it proves that the lepidopteran insect has a magnetic induction mechanism based on free radical pairs.

[0034] Taking the sample size N=15 as an example, we first measured the orientation of Lepidoptera insects under natural conditions, and then used a signal generator to apply a radio frequency magnetic field and measured the orientation again.

[0035] Figure 5 The circular distribution in the figure represents the flight heading of one lepidopteran in the simulator, and the directionality of each lepidopteran's direction is quantified by an r value from 0 to 1. The R value of the outer radius of the plot is 1, indicating the maximum direction orientation; 'N' represents all samples contributing to the experimental data; each red mean vector (MV) is calculated by summing up the average direction and directionality (vector length) of a single lepidopteran, and statistical analysis is performed using the Rayleigh test with Moore's correction; the directionality of the MV is quantified by R * Value quantization; dotted circle indicates p <0.05 andp Significant R<0.01 * Threshold, Figure 5 The outer radius is set to R * = 2.5; the red radial line marks the 95% confidence interval, and the colored ring around the graph indicates the range of this interval.

[0036] like Figure 5 As shown, Figure 5 The left picture shows the group orientation of Lepidoptera insects in natural conditions. The vector sum (red arrow, MV=191.12) points to the south and is longer than the second dotted circle ( p <0.01), the orientation of Lepidoptera insects was more significant, and they tended to orient to the south as a whole; Figure 5 The right figure shows the orientation of Lepidoptera insects after applying the RF magnetic field. The vector sum (MV=162.04) points to the south, but its length is much smaller than the first dotted circle ( p <0.05), the orientation of insects was not significant, and the overall orientation was lost. Compared with the natural situation, the orientation of Lepidoptera insects changed after applying the RF magnetic field, and the orientation was not significant.

[0037] like Figure 6 The flight stability diagram of Lepidoptera is shown in Figure 1. Figure 6 The left picture in the figure shows the angle change during the orientation process of Lepidoptera insects under natural conditions. Figure 6 The right picture in the figure is a comparison of the situation when the RF magnetic field is applied. The Y axis shows the average absolute angle change. Under natural conditions, the average absolute angle change of Lepidoptera insects is small (the average change is 11.7°); after the RF magnetic field is applied, the average absolute angle change of Lepidoptera insects becomes larger (the change value reaches 26.3°), which is more fluctuating than in natural conditions. It is found that the RF magnetic field will affect the flight stability of insects. p This is demonstrated by the value of 0.000545.

[0038] The behavior testing device provided by the present invention can control the electromagnetic coil to generate a specific radio frequency magnetic field signal for experiments through a signal generator, and collect the flight behavior data of the insects to be tested in the experiment to verify the magnetic orientation mechanism of the insect free radical pairs. It has the characteristics of simple structure and simple operation, and is suitable for popularization and use.

[0039] The above embodiments are only for illustrating the technical idea of ​​the present invention, and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution in accordance with the technical idea proposed by the present invention shall fall within the protection scope of the present invention; any technology not involved in the present invention can be realized by existing technologies.

Claims

1. A behavior testing device for identifying insects based on the free radical magnetic induction mechanism, characterized in that: The behavior test device includes a flight orientation test system, a signal acquisition and processing system, and a radio frequency magnetic field signal control system; wherein, The radio frequency magnetic field signal control system is used to modulate and generate a set radio frequency magnetic field signal and act on the insect to be tested in the flight orientation test system (14); The flight orientation test system is used to place the insect to be tested (14), and the insect to be tested (14) that senses the radio frequency magnetic field signal can respond with flight behavior and generate flight behavior data; The signal acquisition and processing system is used to collect the flight behavior data of the insect to be tested (14) generated by the flight orientation test system and to perform analysis and processing.

2. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 1, characterized in that: The flight orientation test system comprises a test box, an electromagnetic shielding cover (4), a micro bearing (5), a carbon fiber rod (6) and an insect hanging ring, wherein the electromagnetic shielding cover (4) is arranged on a box cover (1) of the test box and a micro bearing (5) is embedded in the bottom of the cavity of the electromagnetic shielding cover (4), an optical encoder (2) and an interlocking device (3) are arranged in the inner cavity of the electromagnetic shielding cover (4), the top end of the carbon fiber rod (6) embedded in the micro bearing (5) is connected to the interlocking device (3) upward, the carbon fiber rod (6) extends downward into the box body (9) of the test box, and an insect hanging ring is arranged at the bottom of the carbon fiber rod (6), and the insect hanging ring is used to fix the back of the insect (14) to be tested.

3. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 2, characterized in that: The optical encoder (2) is fixedly mounted in the inner cavity of the electromagnetic shielding cover (4), and the interlocking device (3) arranged in the inner cavity of the electromagnetic shielding cover (4) is capable of rotating along with the carbon fiber rod (6).

4. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 2, characterized in that: The insect hanging ring comprises a polyfluoro tube (15) and a U-shaped copper wire (16); the U-shaped copper wire (16) is arranged at the bottom of the polyfluoro tube (15) and the top of the polyfluoro tube (15) is connected to a carbon fiber rod (6); the size of the U-shaped copper wire (16) is determined according to the body size of the insect (14) to be tested.

5. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 2, characterized in that: The test box is made of nylon material by 3D printing.

6. The device for testing insect behavior based on free radical magnetic induction mechanism according to any one of claims 1 to 5, characterized in that: The signal acquisition and processing system comprises a single-chip microcontroller (8) and a computer (7); the single-chip microcontroller (8) receives flight behavior data of an insect to be tested (14) transmitted by an optical encoder (2) in a flight orientation test system via a shielded signal line (17); the single-chip microcontroller (8) is connected to the computer (7) via a data line; and the computer (7) is used to analyze the flight behavior data of the insect to be tested (14).

7. The device for testing insect behavior based on free radical magnetic induction mechanism according to any one of claims 1 to 5, characterized in that: The radio frequency magnetic field signal control system comprises an electromagnetic coil (10) and a signal generator (11); the electromagnetic coil (10) is wrapped around a test box (9) of a flight orientation test system, and an insect to be tested (14) in the box (9) is located at the center of the electromagnetic coil (10); the electromagnetic coil (10) is connected to a BNC adapter (13) via a shielded signal line (17), and the BNC adapter (13) is installed at the interface of the signal generator (11).

8. The device for testing insect behavior based on free radical magnetic induction mechanism according to claim 7, characterized in that: The electromagnetic coil (10) comprises a coil made of nylon material, on which an enameled copper wire is wound to form the electromagnetic coil (10), and the head and tail of the enameled copper wire are respectively connected to the shielded signal wire (17).

9. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 7, characterized in that: The radio frequency magnetic field signal control system further comprises a Faraday cage (12), and the electromagnetic coils (10) of the flight orientation test system and the radio frequency magnetic field signal control system are both arranged in the Faraday cage (12).

10. The behavior testing device for identifying insects based on the free radical magnetic induction mechanism according to claim 9, characterized in that: The top of the Faraday cage (12) is covered with a copper wire mesh and the Faraday cage is grounded.

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