Anti-wrinkle, firming and repairing composition containing sturgeon caviar extract and application of anti-wrinkle, firming and repairing composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle, firming and repairing composition containing sturgeon caviar extract and application of the anti-wrinkle, firming and repairing composition. According to the anti-wrinkle, firming and repairing composition, the adding amount of the sturgeon caviar extract is increased, and the sturgeon caviar extract is compounded with other functional components, so that the effects of synergistically increasing skin elasticity, smoothing wrinkles, repairing skin barriers, protecting skin injuries and promoting absorption of active substances are achieved, and skin aging is relieved from internal and external dual aspects.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an anti-wrinkle, firming and repairing composition containing a sturgeon caviar extract and application thereof. Background Art

[0002] As we age, the metabolic rate of human cells will gradually decrease. The decrease in the metabolic rate of skin cells will cause the skin to become rough, dry, loose, and wrinkled. However, the causes of human skin aging mainly include internal and external factors. The endogenous factor is mainly the loss of collagen due to aging, which reduces skin elasticity and causes sagging; the exogenous factor is mainly the influence of environmental factors. Long-term exposure to pollutants, radiation, ultraviolet rays and other harmful factors will damage cells and tissues and promote aging. With the improvement of the quality of life, people pay more and more attention to the problem of alleviating facial wrinkles and sagging. However, most traditional anti-aging cosmetics tend to focus on the skin aging process in a single aspect, and lack cosmetics that can systematically alleviate the problem of wrinkles and sagging caused by skin aging.

[0003] Although the prior art already has anti-wrinkle and firming compositions using sturgeon caviar extract as a raw material, the prior art does not use sturgeon caviar extract as the composition component with the largest addition amount, and the anti-wrinkle effect of sturgeon caviar extract cannot be fully exerted. However, simply increasing the addition amount of sturgeon caviar extract can easily lead to reduced stability of the composition or skin intolerance of the user.

[0004] Currently, the art still lacks a high-stability anti-wrinkle and firming repair composition that uses sturgeon caviar extract as a main ingredient and can achieve anti-aging effects from both internal and external levels. Summary of the invention

[0005] The object of the present invention is to provide an anti-wrinkle and firming repairing composition containing sturgeon caviar extract, which uses the sturgeon caviar extract as a main component and can achieve high stability of anti-aging effects from both internal and external factors.

[0006] The present invention provides an anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, comprising the following components in parts by weight:

[0007] 20-50 parts of sturgeon caviar extract, 0.01-0.5 parts of Coleus forskohlii root extract, 0.1-6 parts of type XVII collagen, 0.05-0.5 parts of acetyl hexapeptide-1, 0.1-1 parts of Pyracantha fortuneana fruit extract, and 0.1-5 parts of hydrogenated lecithin.

[0008] Preferably, the following components are included in parts by weight:

[0009] 35-45 parts of sturgeon caviar extract, 0.3-0.5 parts of Coleus forskohlii root extract, 0.1-1 parts of type XVII collagen, 0.05-0.15 parts of acetyl hexapeptide-1, 0.5-1 parts of Pyracantha fortuneana fruit extract, and 0.1-1 parts of hydrogenated lecithin.

[0010] Preferably, the following components are included in parts by weight:

[0011] 40 parts of sturgeon caviar extract, 0.5 parts of Coleus forskohlii root extract, 0.2 parts of type XVII collagen, 0.1 parts of acetyl hexapeptide-1, 0.5 parts of Pyracantha fortuneana fruit extract, and 0.5 parts of hydrogenated lecithin.

[0012] Preferably, the concentration of the sturgeon caviar extract is 2.5 wt %.

[0013] Preferably, the concentration of the Coleus forskohlii root extract is 10 wt %.

[0014] Preferably, the concentration of the Pyracantha fortuneana fruit extract is 10 wt %.

[0015] Preferably, the composition further comprises 40 to 80 parts of auxiliary materials;

[0016] The auxiliary materials include the following raw materials in parts by weight: one or more of 0.05 to 1 part of thickener, 5 to 15 parts of moisturizer, 0.1 to 3 parts of preservative component, 0.01 to 1 part of neutralizer and 20 to 74.83 parts of water.

[0017] The present invention also provides the use of the anti-wrinkle firming repairing composition described in the above technical solution in skin care products.

[0018] Preferably, the dosage form of the skin care product includes any one of a cream, a lotion, a serum and an aqueous solution.

[0019] The present invention also provides an anti-wrinkle, firming and repairing skin care product, comprising the anti-wrinkle, firming and repairing composition described in the above technical solution.

[0020] The beneficial effects of the present invention are:

[0021] The present invention provides an anti-wrinkle and firming repairing composition containing sturgeon caviar extract. By adjusting the ratio of raw materials, sturgeon caviar is used as the raw material with the largest amount of addition in the composition. Through the ratio between the raw materials, the stability and safety of the composition after the addition amount of sturgeon caviar extract is increased can be ensured, and the anti-wrinkle and firming repairing effect is maximized. Sturgeon caviar extract is rich in various proteins, vitamins, fatty acids and amino acids. Sturgeon caviar extract has a high level of fatty acids, including polyunsaturated fatty acids (PUFAs), DHA and eicosapentaenoic acid (EPA), and is rich in nutritional value.

[0022] The sturgeon caviar extract of the present invention can promote the expression of collagen ColⅠ, promote the proliferation of fibroblasts, and inhibit the secretion of MPP-1. The composition of the present invention has a good antioxidant effect, can increase skin elasticity, reduce wrinkles, repair skin barriers, protect skin damage, and achieve a more comprehensive relief of skin aging problems.

[0023] The compositions of the present invention have a synergistic effect and can systematically alleviate skin aging problems through multiple pathways such as activation, repair, and stabilization, thereby achieving the effect of increasing the source of skin collagen and reducing the consumption, and slowing down the loss of skin collagen, thereby achieving the effects of increasing skin elasticity, fading wrinkles, repairing the skin barrier, protecting skin damage, and promoting the absorption of active substances. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0025] Figure 1 This is a schematic diagram of the test process of the elastase inhibition test of sturgeon caviar extract in Example 6;

[0026] Figure 2 This is a graph showing the growth of human skin fibroblasts tested with sturgeon caviar extract in Example 8;

[0027] Figure 3 This is a bar graph showing the content of type I collagen at each time point in the efficacy test of the sturgeon caviar extract in Example 9;

[0028] Figure 4 This is a bar graph showing the MMP-1 inhibition rate at each time point for the efficacy of the sturgeon caviar extract in Example 10;

[0029] Figure 5 This is a bar graph showing the percentage of under-eye wrinkle area of ​​the anti-wrinkle firming repair essence of the composition (10%) in Example 12;

[0030] Figure 6 This is a diagram showing the human body test effect of the anti-wrinkle, firming and repairing essence of the composition (10%) in Example 12. DETAILED DESCRIPTION

[0031] The present invention provides an anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, comprising the following components in parts by weight:

[0032] 20-50 parts of sturgeon caviar extract, 0.01-0.5 parts of Coleus forskohlii root extract, 0.1-6 parts of type XVII collagen, 0.05-0.5 parts of acetyl hexapeptide-1, 0.1-1 parts of Pyracantha fortuneana fruit extract, and 0.1-5 parts of hydrogenated lecithin.

[0033] As an embodiment, the anti-wrinkle and firming repair composition of the present invention includes the following components in parts by weight: 35-45 parts of sturgeon caviar extract, 0.3-0.5 parts of Coleus forskohlii root extract, 0.1-1 parts of type XVII collagen, 0.05-0.15 parts of acetyl hexapeptide-1, 0.5-1 parts of Pyracantha fortuneana fruit extract, and 0.1-1 parts of hydrogenated lecithin.

[0034] In parts by weight, as an embodiment, the present invention includes 20 to 50 parts of sturgeon caviar extract, the concentration of the sturgeon caviar extract is 2.5wt%, and the concentration is the concentration of the sturgeon caviar extract itself; as an embodiment, the weight of the sturgeon caviar of the present invention can be any one of 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts and 50 parts; in a specific embodiment, the amount of the sturgeon caviar is 40 parts, and the concentration of the sturgeon caviar extract is 2.5wt% (purchased from Zhuhai Jiayan Biotechnology Co., Ltd.). The sturgeon caviar extract of the present invention is extracted from sturgeon eggs, rich in protein, fatty acids, amino acids, vitamins and minerals, etc., and has the function of anti-aging skin.

[0035] In parts by weight, as an embodiment, the present invention includes 0.01 to 0.5 parts of a root extract of Coleus forskohlii, and the concentration of the root extract of Coleus forskohlii is 10wt% (purchased from Zhuhai Jiayan Biotechnology Co., Ltd.); as an embodiment, the weight proportion of the root extract of Coleus forskohlii of the present invention can be any one of 0.01 parts, 0.05 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts and 0.5 parts; in a specific embodiment, the amount of the root extract of Coleus forskohlii is 0.5 parts, and the concentration is 10wt%. The Coleus forskohlii root extract of the present invention is extracted from the Coleus forskohlii root, contains an adenylate cyclase agonist, can activate the increase of intracellular cyclic adenosine monophosphate (cAMP) level, thereby inhibiting the biological activity of stress activated protein (SAPK) in the cell, and then stimulating intracellular mitochondrial biosynthesis, supplying intracellular energy ATP and other nutrients, and other various skin nutrition small molecule active proteins, can promote endogenous skin growth and deep repair and renewal, and finally play an anti-skin aging effect.

[0036] In parts by weight, as an embodiment, the present invention includes 0.1 to 6 parts of type XVII collagen; as an embodiment, the weight of the type XVII collagen can be any one of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5 and 6 parts; in a specific embodiment, the amount of type XVII collagen is 0.2 parts. The type XVII collagen in the present invention can be used as an important transmembrane protein in the basement membrane region of the epidermis, can maintain the self-renewal and proliferation of epidermal stem cells, regulate the correct development and migration of epidermal cells, thereby maintaining the order of the epidermis, promoting the expression of basement membrane-related proteins, and achieving the purpose of strengthening the skin and delaying aging. Applied in cosmetics, type XVII collagen can promote the regeneration of COLⅣ and COLXVII collagen, repair the basement membrane zone, regulate cell self-purification, promote the expression of ColⅠ and ColⅢ collagen, and increase collagen content.

[0037] In parts by weight, as an embodiment, the present invention includes 0.05 to 0.5 parts of acetyl hexapeptide-1; as an embodiment, the weight of the acetyl hexapeptide-1 can be any one of 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts and 0.5 parts; in a specific embodiment, the weight of the acetyl hexapeptide-1 is 0.1 parts. The acetyl hexapeptide-1 in the present invention can reduce the transmission of neurotransmitters and reduce muscle shrinkage by acting on the presynaptic membrane and the postsynaptic membrane at the same time, thereby achieving the purpose of smoothing expression lines and achieving anti-aging effects.

[0038] In terms of weight, as an embodiment, the present invention includes 0.1 to 1 parts of Pyracantha fortuneana fruit extract, and the concentration of the Pyracantha fortuneana fruit extract is 10wt% (purchased from Zhuhai Jiayan Biotechnology Co., Ltd.); as an embodiment, the weight of the Pyracantha fortuneana fruit extract can be any one of 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts and 1.0 parts; in a specific embodiment, the weight of the Pyracantha fortuneana fruit extract is 0.5 parts. The Pyracantha fortuneana fruit extract of the present invention is extracted from the fruit of Pyracantha fortuneana in Yunnan, is rich in flavonoids (quercetinglycosides) and tannins, and is used in cosmetics to have multi-mechanism antioxidant, free radical scavenging ability, enhance antioxidant enzyme activity and reduce the level of reactive oxygen species ROS.

[0039] In terms of weight, as an embodiment, the present invention includes 0.1 to 5 parts of hydrogenated lecithin; as an embodiment, the weight of the hydrogenated lecithin of the present invention can be any one of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4 and 5 parts; in a specific embodiment, the weight of the hydrogenated lecithin is 0.5 parts. The hydrogenated lecithin of the present invention is a stearic acid glyceride-like solid, and its stability, hydrophilicity, emulsification, permeability, wettability and dispersibility are superior to those of natural lecithin.

[0040] As an embodiment, the anti-wrinkle firming repair composition further comprises 40 to 80 parts of auxiliary materials;

[0041] The auxiliary materials include the following raw materials in parts by weight: one or more of a thickener, a humectant, a preservative, a neutralizer, an antioxidant and water; in a specific embodiment, the auxiliary materials include a humectant, a preservative and water.

[0042] In parts by weight, as an embodiment, the weight portion of the thickener in the anti-wrinkle firming repair composition is 0.05 parts to 1 parts; as an embodiment, the weight portion of the thickener can be any one of 0.05 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts and 1 parts; the thickener can be any one or more of carbomer, ammonium acryloyldimethyltaurate / VP copolymer, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer, sodium polyacrylate, polyacrylate cross-linked polymer and glycerol polyacrylate; as an embodiment, when the thickener is multiple, the weight ratio between different thickeners is not particularly limited.

[0043] In terms of parts by weight, as an embodiment, the parts by weight of the moisturizer in the anti-wrinkle firming repair composition are 5 to 15 parts; in one embodiment, the parts by weight of the moisturizer can be any one of 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15 parts; as an embodiment, the moisturizer can be any one or more of glycerol, butylene glycol, propylene glycol, sodium hyaluronate, dipropylene glycol, panthenol, glyceryl polyether-26, diglycerol, sorbitol, erythritol, PEG-8, PEG-32, PEG-6 and PEG-15; as an embodiment, when there are multiple thickeners, the weight ratio between different thickeners is not particularly limited; in a specific embodiment, the moisturizer is glycerol, and the parts by weight of the glycerol is 10.8 parts.

[0044] In parts by weight, as an embodiment, the weight proportion of the preservative in the anti-wrinkle firming repair composition is 0.1 to 3 parts; as an embodiment, the amount of the preservative can be any one of 0.1, 0.15, 0.2, 0.25, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2 and 3 parts; as an embodiment, the preservative can be any one or more of 1,2-pentanediol, 1,2-hexanediol, phenoxyethanol, ethylhexylglycerin, parabens, sodium benzoate, caprylhydroxamic acid, methylparaben and iodopropynyl butylcarbamate; as an embodiment, when the thickener is multiple, the weight ratio between different thickeners is not particularly limited; in the example composition, the preservative is 1,2-hexanediol, and the weight proportion of the 1,2-hexanediol is 1 part.

[0045] In parts by weight, as an embodiment, the anti-wrinkle firming repair composition also includes 0.01 to 1 parts by weight of a neutralizer; as an embodiment, the weight of the neutralizer can be any one of 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0 parts; as an embodiment, the neutralizer of the present invention includes any one of sodium hydroxide, citric acid, potassium hydroxide, sodium bicarbonate and arginine; in a specific embodiment, the neutralizer is arginine, and the weight of arginine is 0.01 parts.

[0046] In parts by weight, as an embodiment, the anti-wrinkle firming repair composition further includes an antioxidant in an amount of 0.01 to 1 part by weight; as an embodiment, the antioxidant can be in any one of 0.01, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0 parts by weight; as an embodiment, the antioxidant includes any one of p-hydroxyacetophenone, ascorbic acid (vitamin C), silymarin and glutathione; in a specific embodiment, the antioxidant is p-hydroxyacetophenone, and the weight portion of p-hydroxyacetophenone is 1 part.

[0047] In terms of parts by weight, as an embodiment, the anti-wrinkle firming repair composition also includes 20 parts to 74.83 parts by weight of water. As an embodiment, the weight of the water can be any one of 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 71 parts, 72 parts, 73 parts, 74 parts and 74.83 parts; in a specific embodiment, the weight of the water is 60 parts.

[0048] The present invention also provides a method for preparing the anti-wrinkle and firming repairing composition described in the above technical scheme, comprising the following steps: weighing each raw material according to the weight ratio of the raw materials of the anti-wrinkle and firming repairing composition, and fully mixing the raw materials to obtain a mixture, which is a composition with anti-wrinkle and firming repairing effects.

[0049] The present invention also provides the use of the anti-wrinkle firming repairing composition described in the above technical solution in skin care products.

[0050] In terms of weight percentage, as an embodiment, the weight percentage of the anti-wrinkle and firming repairing composition in the anti-wrinkle and firming repairing skin care product of the present invention is 1-10%; as an embodiment, the weight percentage of the anti-wrinkle and firming repairing composition in the anti-wrinkle and firming repairing skin care product can be any one of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% and 10%; in a specific embodiment, the weight percentage of the anti-wrinkle and firming repairing composition in the anti-wrinkle and firming repairing skin care product is 10%.

[0051] As an embodiment, the dosage form of the skin care product can be any one of a cream, a lotion, an essence and an aqueous solution. In a specific embodiment, the dosage form of the skin care product is an essence.

[0052] The present invention also provides a raw material composition of an anti-wrinkle firming repair essence. As an embodiment, the anti-wrinkle firming repair essence comprises an anti-wrinkle firming repair composition and auxiliary materials; as an embodiment, the auxiliary materials comprise one or more of a thickener, a moisturizer, a preservative, a neutralizer, an antioxidant and water; in a specific embodiment, the anti-wrinkle firming repair composition in the anti-wrinkle firming repair essence is 10 parts by weight, the thickener is glycerol polyacrylate, the weight portion of the glycerol polyacrylate is 0.7 parts, the moisturizer is glycerol, butylene glycol, propylene glycol, etc. The weight proportion of the glycerin is 2.5 parts, the weight proportion of the butylene glycol is 3.3 parts, the weight proportion of the propylene glycol is 5 parts, the weight proportion of the sodium hyaluronate is 0.05 parts, the preservative is phenoxyethanol, the weight proportion of the phenoxyethanol is 0.25 parts, the neutralizer is arginine, the weight proportion of arginine is 0.01 parts, the antioxidant is p-hydroxyacetophenone, the weight proportion of p-hydroxyacetophenone is 0.5 parts, and the deionized water is 77.69 parts.

[0053] The present invention also provides the above-mentioned anti-wrinkle, firming and repairing skin care product, comprising any of the above-mentioned compositions.

[0054] In order to further illustrate the present invention, an anti-wrinkle and firming repairing composition containing sturgeon caviar extract and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0055] Example 1 An anti-wrinkle and firming repairing composition containing sturgeon caviar extract

[0056] The weight proportions of the components in the anti-wrinkle firming repair composition are as follows:

[0057] 20 parts of sturgeon caviar extract, 0.8 parts of Coleus forskohlii root extract, 0.2 parts of collagen (type XVII collagen), 10.2 parts of acetyl hexapeptide-1, 0.1 parts of Pyracantha fortuneana fruit extract, 0.8 parts of hydrogenated lecithin, 10.8 parts of glycerol, and 1 part of 1,2-hexanediol.

[0058] Example 2 An anti-wrinkle and firming repairing composition containing sturgeon caviar extract

[0059] The weight proportions of the components in the anti-wrinkle firming repair composition are as follows:

[0060] 30 parts of sturgeon caviar extract, 0.6 parts of Coleus forskohlii root extract, 0.1 parts of collagen (type XVII collagen), 10.1 parts of acetyl hexapeptide-1, 0.8 parts of pyracantha fruit extract, 0.6 parts of hydrogenated lecithin, 10.8 parts of glycerol, and 1 part of 1,2-hexanediol.

[0061] Example 3 An anti-wrinkle and firming repairing composition containing sturgeon caviar extract

[0062] The weight proportions of the components in the anti-wrinkle firming repair composition are as follows:

[0063] 40 parts of caviar extract, 0.5 parts of Coleus forskohlii root extract, 0.2 parts of collagen (type XVII collagen), 10.1 parts of acetyl hexapeptide-1, 0.5 parts of pyracantha fruit extract, 0.5 parts of hydrogenated lecithin, 10.8 parts of glycerol, and 1 part of 1,2-hexanediol.

[0064] Example 4: An anti-wrinkle and firming repairing composition containing sturgeon caviar extract

[0065] The weight proportions of the components in the anti-wrinkle firming repair composition are as follows:

[0066] 50 parts of sturgeon caviar extract, 0.2 parts of Coleus forskohlii root extract, 0.1 parts of collagen (type XVII collagen), 10.05 parts of acetyl hexapeptide-1, 0.2 parts of pyracantha fruit extract, 0.5 parts of hydrogenated lecithin, 10.8 parts of glycerol, and 1 part of 1,2-hexanediol.

[0067] Example 5 Preparation method of an anti-wrinkle and firming repairing composition containing sturgeon caviar extract

[0068] The steps of the preparation method of the anti-wrinkle firming repair composition are as follows:

[0069] Weigh the raw materials according to the weight ratio described in any one of Examples 1 to 4, and fully mix the raw materials to obtain a mixture, which is a composition with anti-wrinkle, firming and repairing effects.

[0070] Example 6 Verification of the effect of sturgeon caviar extract on elastase

[0071] Verification method: The anti-wrinkle and firming repairing compositions described in Examples 1 to 4 were prepared according to the method described in Example 5, and the concentration of the compositions was diluted to 10% with purified water to obtain test samples 1 to 4. Purified water was used as a negative control group, and the obtained test samples 1 to 4 and the negative control group were subjected to an elastase inhibition test. The test results are shown in Table 1.

[0072] The test method for the elastase inhibition test is as follows Figure 1As shown, the specific steps include the following: Elastase can catalyze the hydrolysis of hydroxyl groups contained in polypeptide bonds in various amino acids, so when reacting with the substrate N-succinyl-alanine-alanine-alanine-p-nitroaniline (CAS No.: 52299-14-6), a colored substance will be produced. Therefore, after adding the test samples 1 to 4 to the reaction tube, elastase and buffer are added and mixed, the mixed solution is heated in a water bath at 25°C for 15 minutes, AAAPAN is added and mixed, and the mixed solution is heated in a water bath at 25°C for 15 minutes again. The absorbance value of the heated liquid can be measured at 410nm in a spectrophotometer. According to the change in absorbance, the inhibitory effect of the test substance on the activity of elastase is calculated. The calculation formula is shown below, which can evaluate whether the test substance has firming and anti-wrinkle effects.

[0073] The calculation formula of elastase inhibition rate was: [1-(CD) / (AB)]×100%.

[0074] Where: C is the absorbance value of the sample group, that is, the absorbance value of the solution after the sample affects the reaction between elastase and substrate AAAPAN;

[0075] D is the absorbance value of the sample background group;

[0076] A is the absorbance of the enzyme reaction group, that is, the absorbance of the reaction between elastase and substrate AAAPAN when no sample is added;

[0077] B is the absorbance value of the solvent background group.

[0078] Table 1 Determination results of the inhibition rate of elastase by the anti-wrinkle firming repair composition

[0079] Group Elastase inhibition rate (%) Sample 1 16.34 Sample 2 22.14 Sample 3 42.21 Sample 4 36.45 Negative control group 4.40

[0080] Elastin plays a key role in the youthfulness of the skin, and the lack of elastin is the main cause of skin aging. Inhibiting the activity of elastase can effectively slow down the decomposition of elastin and maintain the elasticity of the skin. As shown in Table 1, the elastase inhibition rates of the test samples 1-4 of the present invention are 16.34%, 22.14%, 42.21%, and 36.45%, respectively, while the elastase inhibition rate of the negative control group is only 4.40%. It can be seen that when the concentration of the anti-wrinkle and firming repairing composition is 10%, it has the ability to inhibit the activity of elastase, thereby proving that the anti-wrinkle and firming repairing composition of the present invention has an anti-wrinkle and firming effect.

[0081] Example 7 Antioxidant effect test of sturgeon caviar extract using ABTS method

[0082] Reference for antioxidant effect test method: GB / T 39100-2020 "DPPH and ABTS method for testing antioxidant activity of polypeptides".

[0083] Clearance calculation formula: Clearance = [(absorbance of sample group - absorbance of blank group) / absorbance of sample group] × 100%

[0084] The stable free radical ABTS+ solution is blue-green and has a maximum absorption at 734nm. This free radical can be generated by the reaction of ABTS and K2S2O8. When the free radical is removed and the number decreases, the color of its solution will become lighter and the absorbance at 734nm will decrease, which can be used to judge the ability of the sample to remove ABTS+.

[0085] The test results are as follows: when the sturgeon caviar extract is diluted to a 4% solution, the free radical scavenging rate is 96.80%; this shows that the sturgeon caviar extract has a good antioxidant effect.

[0086] Example 8 Cell proliferation test using sturgeon caviar extract

[0087] Normal mammalian cells cultured in vitro continue to divide and proliferate. Toxic substances, regardless of their site of action and mechanism, will interfere with the cell division and proliferation process, resulting in a decrease in cell growth rate and number. Sturgeon caviar extracts at concentrations of 0.0871%, 0.156%, and 0.313% were added to human immortalized epidermal cells (HaCaT) cultured in vitro, and the cell proliferation efficiency was determined using an ELISA reader set to OD450nm for reading detection.

[0088] The test method is as follows:

[0089] Pretreatment of the test substance: The sample (sturgeon caviar extract) stock solution was sterilized and filtered using a 0.22 μm filter, and then diluted to the required concentration using culture medium.

[0090] Cell proliferation test: Select cells with appropriate digestion status, count the cells and then plate them. 24 hours after plating, use a spray gun to add the test substance to make its final concentration ≤IC10, set up a solvent control, and set up parallel test wells n≥4. 0, 24, 48, 72 hours after adding the sample, add the culture medium containing 10% CCK-8, and culture accurately for 2 hours ± 5 minutes. Use an ELISA reader to set OD450nm for reading detection.

[0091] The cell survival rate calculation formula is:

[0092] Survival rate (%) = [(OD cnH -OD VCnHb ) / (OD c0H -OD VC0Hb )]×100.

[0093] Wherein: CnH refers to the detection group at nH, containing the test substance and cells; C0H refers to the detection group at 0H, solvent control, without the test substance and cells; VCb-solvent blank control, without the test substance and cells.

[0094] The test results are shown in Table 2 and Figure 2 As shown;

[0095] Table 2 Results of cell proliferation test of sturgeon caviar extract

[0096]

[0097] The cell proliferation curve was obtained according to the results of the culture time and viable cell number test in Table 2. It can be seen from Table 6 that Figure 2 It can be seen that compared with the blank control group, adding 0.078%, 0.156%, and 0.313% of the test substance sturgeon caviar extract can significantly promote the growth of HaCaT.

[0098] Example 9: Experiment on the inhibition of MMP-1 (matrix metalloproteinase) secretion using sturgeon caviar extract

[0099] UV irradiation of fibroblasts (HDF) can cause cell damage, a large amount of inflammatory factors secreted, and a decrease in cell matrix secretion. Detecting the effect of sturgeon caviar extract on cell-related indicators of light damage can serve as evidence to support the sample's ability to repair light damage.

[0100] The detection method is as follows:

[0101] Cell plating: After counting the cells, dilute to the required concentration and add the cell suspension to a 96-well microplate. 24 hours after plating, the cell fusion rate is 40-60%.

[0102] The test groups were set up as follows: the BC group was not treated; the NC group was treated with UV damage; the experimental group was treated with UV damage + different concentrations of sturgeon caviar extract samples;

[0103] ELISA test was performed on different test groups: 200 μL of fibroblast culture was collected from each well, the supernatant of the cultured fibroblasts was aspirated and placed in a 1.5 mL sterile centrifuge tube, and the test was performed according to the instruction manual of the MMP-1 ELISA test kit.

[0104] MMP-I content calculation: With the absorbance OD value as the ordinate (Y), and the corresponding concentration of the test substance standard as the abscissa (X), a corresponding curve is made. The test substance content of the sample can be converted to the corresponding concentration from the standard curve according to its OD value. Finally, the average value of the three replicate wells in each group is taken as the final test substance content result, and the standard deviation is calculated. The test results are shown in Table 3 and Figure 3 As shown:

[0105] Table 3 Results of MMP-I secretion of caviar extract on UV-HDF

[0106]

[0107] From Table 3 and Figure 3 It can be seen that, compared with the injury group, the addition of 0.0781%, 0.1563%, and 0.3125% of the test substance sturgeon caviar extract can significantly inhibit the secretion of MMP-1 (P<0.01).

[0108] Example 10 Testing the content of collagen Col Ⅰ using sturgeon caviar extract

[0109] When dermal fibroblasts are cultured in vitro, fibroblasts will secrete collagen during the process of division and proliferation, and toxic substances will interfere with the secretion of cell collagen.

[0110] The present invention determines the expression of collagen after administration of the test object sturgeon caviar extract to evaluate whether the test object has the effect in firming and anti-wrinkle cell evaluation.

[0111] This test uses human fibroblasts HDF and human ELISA kit to detect type I collagen (Collagen Ⅰ, hereinafter referred to as Col Ⅰ) in sturgeon caviar extract. Type I collagen specifically binds to the specific antibody coated on the ELISA plate to produce different shades of color. The optical density (OD value) of the color is measured at a wavelength of 450nm using an ELISA reader to calculate the content of type I collagen.

[0112] Experimental steps: dilute to the required concentration, add the cell suspension to the 96-well microplate, and when the cell fusion rate reaches 60-70% after 24 hours of plating, use a spray gun to add the test substance to make its final concentration the set concentration, set the solvent control, and set the test wells in parallel to no less than 3. After 24±2 hours of sample addition and incubation, collect 200μL of the supernatant of the cultured fibroblasts from each well, place it in a 1.5mL sterile centrifuge tube, and test according to the instructions for use of the enzyme-linked immunosorbent assay kit. Before the formal test, it is necessary to set the dilution ratio, conduct a preliminary experiment, and explore and determine the dilution ratio of the ELISA test group to ensure that the test value falls within the standard curve range.

[0113] Calculation of type Ⅰ collagen content:

[0114] The absorbance OD value is the ordinate (Y), and the corresponding concentration of the standard substance to be tested is the abscissa (X). The corresponding curve is made. The content of the substance to be tested in the sample can be converted into the corresponding concentration from the standard curve according to its OD value. Finally, the average value of the three replicate wells in each group is taken as the final result of the content of the substance to be tested. The test results are shown in Table 4 and Figure 4 .

[0115] Calculation of the upregulation rate of type Ⅰ collagen content: Upregulation rate (%) = [(T / C)-1] × 100

[0116] Where: T is the average content of the substance to be tested in the test wells; C is the average content of the substance to be tested in the blank / solvent control wells.

[0117] Table 4 Effect of sturgeon caviar extract on type Ⅰ collagen content in fibroblasts

[0118]

[0119] Note: In Table 4, P < 0.05 represents a significant difference, and P < 0.01 represents an extremely significant difference.

[0120] From Table 4 and Figure 4 It can be seen that when the TGF-β1 in the PC group was tested at a concentration of 75ng / mL, the upregulation rate of type I collagen content in HDF was 25.00±2.05%, and the data were significantly different from those in the NC group (p<0.05), indicating that the experimental model was effective. The upregulation rates of type I collagen content in HDF by the test substance AAG-101 at concentrations of 0.0781%, 0.1563%, and 0.3125% were 104.02±28.35%, 104.99±9.79%, and 153.62±19.94%, respectively, and the data were significantly different (P<0.01).

[0121] Comparative Example 1

[0122] An anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, the number of raw materials is the same as that of Example 3, except that it does not contain Coleus forskohlii root extract.

[0123] Comparative Example 2

[0124] An anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, the number of raw materials is the same as that of Example 3, except that collagen (type XVII collagen) is not contained.

[0125] Comparative Example 3

[0126] An anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, the number of raw materials is the same as that of Example 3, except that acetyl hexapeptide-1 is not contained.

[0127] Comparative Example 4

[0128] An anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, the number of raw materials is the same as that of Example 3, except that it does not contain Pyracantha fortuneana fruit extract.

[0129] Comparative Example 5

[0130] An anti-wrinkle, firming and repairing composition containing sturgeon caviar extract, the raw material parts are the same as those in Example 3, but the composition does not contain hydrogenated lecithin.

[0131] The specific raw material added components of the compositions of Examples 1-4 and Comparative Examples 1-5 are shown in Table 5.

[0132] Table 5 Composition components of Examples 1-4 and Comparative Examples 1-5

[0133]

[0134] The anti-wrinkle and firming repairing compositions of the ingredients described in Examples 1 to 4 and Comparative Examples 1 to 4 were prepared by the preparation method described in Example 5, and then the anti-wrinkle and firming repairing essence was prepared according to the percentage of raw material components shown in Table 6.

[0135] Table 6 Anti-wrinkle firming repair essence composition table

[0136]

[0137]

[0138] Example 11 Safety test of anti-wrinkle firming repair essence

[0139] The anti-wrinkle firming repair essences 1 to 13 shown in Table 6 were subjected to a human skin closed patch test to evaluate their safety.

[0140] The human skin closed patch test was based on the test requirements for closed patches in the 2015 edition of the "Technical Specifications for Safety of Cosmetics", and essences 1-13 were tested; the negative control was deionized water, and a total of 66 subjects were randomly divided into 2 groups, with 33 subjects in each group. The average age of Group 1 was 36.39±6.09 years old, and the effects of essences 1 to 7 were tested; the average age of Group 2 was 36.82±3.96 years old, and the effects of essences 8 to 13 were tested. The test results are shown in Table 7.

[0141] Table 7 Score levels of different skin reactions in patch tests of serums 1 to 13

[0142]

[0143]

[0144] As shown in Table 7, during the test, 2 and 5 subjects had grade 1 skin reactions in Essence 6 and Essence 7 respectively after 0.5 hour in the spot removal tester. Considering the product safety issue, it is recommended that the amount of the anti-wrinkle and firming repair composition added to cosmetics should not exceed 10%.

[0145] Example 12 Effect test of essence

[0146] At room temperature (21±1)°C and humidity 40-60%, the effects of essences 1 to 5 and 8 to 13 were tested;

[0147] Subjects: 220 volunteers, aged 30-45 years old, 161 females and 59 males, randomly divided into 11 groups, 20 people in each group, double-blind, randomized half-face, self-controlled before and after observation study. Subjects applied the test sample on one side of the face and the placebo on the other side of the face according to the random table. The product use cycle is 28 days, and the product use frequency is once in the morning and once in the evening every day. Subjects were clinically tested and evaluated on D0, D14, and D28 after inclusion in the study. During the test period, the subjects did not experience erythema, papules, wheals, itching and other skin adverse reactions associated with the trial products.

[0148] Test indicators and test instruments: Use Delfin elasticity tester, Delfin stratum corneum moisture content tester, and Delfin transepidermal water loss tester to test facial elasticity, facial skin stratum corneum moisture content, and facial skin transepidermal water loss rate respectively; use VISIA skin analyzer to capture facial images, and use IPP software to analyze changes in facial fine lines and other conditions.

[0149] After using the essence for 28 days, the improvement effect of the essence on the skin was tested. The results of the effect of the essence on skin elasticity are shown in Table 8 and Figures 5-6 The improvement effect of the essence on wrinkles is shown in Table 9; the effect of the essence on skin barrier repair is shown in Table 10.

[0150] Table 8 Skin elasticity test results after using the essence for 4 weeks

[0151]

[0152] According to Table 8 and Figure 5 The results show that the essences 1, 2, and 5 of the present invention have an improvement effect on the skin elasticity of the cheeks, under the eyes, corners of the eyes, and corners of the mouth, and the essences 3 and 8 have a more significant improvement effect on the skin elasticity of the cheeks, under the eyes, corners of the eyes, and corners of the mouth. However, the essence 4 group cannot achieve the ideal skin elasticity improvement effect due to the low amount of anti-wrinkle and firming repair composition added. Although the essences 9 to 10 prepared from the anti-wrinkle and firming repair compositions described in comparative examples 1 to 5 also have a certain improvement effect on the skin elasticity, their improvement effects are far lower than the improvement effects of the essences 1 to 3, 5, and 8 prepared in the examples of the present invention on the skin elasticity.

[0153] Table 9 Improvement rate of fine lines under the eyes and crow's feet by essence

[0154] Group Improvement rate of fine lines under eyes (%) Crow's feet improvement rate (%) Essence 1 25.11 22.29 Essence 2 27.47 26.26 Essence 3 30.92 31.71 Essence 4 11.57 12.53 Essence 5 19.71 21.83 Essence 8 28.10 26.94 Essence 9 10.54 10.51 Essence 10 9.03 9.56 Essence 11 8.77 9.12 Essence 12 9.98 10.04 Essence 13 6.10 7.94

[0155] According to Table 9 and Figure 6The results show that essences 1 to 3, 5 and 8 of the present invention have obvious improvement effects on fine lines under the eyes and crow's feet, and essence 3 has the most significant improvement effect on wrinkles, reaching more than 30%; although essences 4, 9 to 13 also have a certain improvement effect on wrinkles, the improvement effect is not significant. It can be seen that the raw materials in the present invention have a synergistic effect, and the raw material combination of the present invention can improve the improvement rate of the essence on wrinkles.

[0156] Table 10 Results of the effect of serum on improving skin barrier

[0157] Group Improvement rate of stratum corneum moisture content (%) Transepidermal water loss rate improvement rate (%) Essence 1 55.72 16.48 Essence 2 61.82 19.24 Essence 3 74.61 28.44 Essence 4 42.55 8.81 Essence 5 45.51 14.48 Essence 8 63.74 22.39 Essence 9 29.68 7.37 Essence 10 35.91 8.10 Essence 11 40.50 8.59 Essence 12 34.96 7.93 Essence 13 22.70 5.05

[0158] According to Table 10, it can be seen that essences 1 to 3, 5 and 8 of the present invention have obvious repairing effects on the skin barrier, and essence 3 has the most obvious repairing effect on the skin barrier, with an improvement rate of up to 74.61% on the moisture content of the skin stratum corneum and a 28.44% improvement rate on the transepidermal water loss rate; while essences 4, 9 to 13 with some raw materials deleted have unsatisfactory repairing effects on the skin barrier, with a maximum improvement rate of only 40.50% on the moisture content of the skin stratum corneum and a maximum improvement rate of only 8.59% on the transepidermal water loss rate; it can be seen that the raw materials in the present invention have a synergistic effect, which can improve the repairing effect of the essence on the skin barrier.

[0159] In summary, the anti-wrinkle, firming and repairing composition containing caviar extract prepared by the present invention has the effects of reducing wrinkles, firming skin and repairing skin barrier. The cosmetics prepared from the composition have excellent anti-aging effects. At the same time, the present invention verifies that the anti-aging mechanism of caviar extract is: by promoting the expression of collagen ColⅠ, promoting the proliferation of fibroblasts, inhibiting the secretion of MPP-1 and its good antioxidant effect, the effects of increasing skin elasticity, reducing wrinkles, repairing skin barrier and protecting skin damage are achieved.

[0160] The above description is only a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements without departing from the principle of the present invention, and these improvements should also be regarded as within the protection scope of the present invention.

Claims

1. An anti-wrinkle and firming repairing composition containing sturgeon caviar extract, characterized in that: The composition comprises the following components in parts by weight: 20-50 parts of sturgeon caviar extract, 0.01-0.5 parts of Coleus forskohlii root extract, 0.1-6 parts of type XVII collagen, 0.05-0.5 parts of acetyl hexapeptide-1, 0.1-1 parts of Pyracantha fortuneana fruit extract, and 0.1-5 parts of hydrogenated lecithin.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 35-45 parts of sturgeon caviar extract, 0.3-0.5 parts of Coleus forskohlii root extract, 0.1-1 parts of type XVII collagen, 0.05-0.15 parts of acetyl hexapeptide-1, 0.5-1 parts of Pyracantha fortuneana fruit extract, and 0.1-1 parts of hydrogenated lecithin.

3. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 40 parts of sturgeon caviar extract, 0.5 parts of Coleus forskohlii root extract, 0.2 parts of type XVII collagen, 0.1 parts of acetyl hexapeptide-1, 0.5 parts of Pyracantha fortuneana fruit extract, and 0.5 parts of hydrogenated lecithin.

4. The composition according to claim 1, characterized in that The concentration of the sturgeon caviar extract is 2.5 wt %.

5. The composition according to claim 1, characterized in that The concentration of the Coleus forskohlii root extract is 10 wt %.

6. The composition according to claim 1, characterized in that The concentration of the Pyracantha fortuneana fruit extract is 10 wt %.

7. The composition according to claim 1, characterized in that The composition also includes 40 to 80 parts of auxiliary materials; The auxiliary materials include the following raw materials in parts by weight: one or more of 0.05 to 1 part thickener, 5 to 15 parts moisturizer, 0.1 to 3 parts preservative, 0.01 to 1 part neutralizer, 0.01 to 1 part antioxidant and 20 to 74.83 parts water.

8. Use of the anti-wrinkle and firming repairing composition according to any one of claims 1 to 7 in skin care products.

9. The use according to claim 8, characterized in that The dosage form of the skin care product includes any one of cream, lotion, essence and toner.

10. An anti-wrinkle, firming and repairing skin care product, characterized in that: The invention comprises the anti-wrinkle and firming repairing composition according to any one of claims 1 to 7.

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